LOCUS       BGC0002148             12582 bp    DNA     linear   BCT 07-JUN-2022
DEFINITION  Sphaerospermopsis torques-reginae ITEP-024 chromosome.
ACCESSION   BGC0002148
VERSION     BGC0002148
KEYWORDS    .
SOURCE      Sphaerospermopsis torques-reginae ITEP-024
  ORGANISM  Sphaerospermopsis torques-reginae ITEP-024
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales;
            Aphanizomenonaceae; Sphaerospermopsis; Sphaerospermopsis
            torques-reginae.
COMMENT     Bacteria and source DNA available from University of Sao Paulo,
            Center for Nuclear Energy in Agriculture, Address: 303 Centenario
            Ave, Piracicaba - SP, 13400-970, Brazil.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:02:37
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12582
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     subregion       1..12582
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002148"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12582
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(6..755)
                     /gene="gntJ"
                     /locus_tag="K2F26_16450"
     CDS             complement(6..755)
                     /codon_start=1
                     /gene="gntJ"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /locus_tag="K2F26_16450"
                     /note="GntJ, class I SAM-dependent methyltransferase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="guanitoxin forming phosphate O-methyltransferase"
                     /protein_id="QYX30480.1"
                     /transl_table=11
                     /translation="MVKSNILSRNVVGYNSTDLSSDHIQAEARDFYARQAGVFAKILNE
                     SWSEYTNISHPGFKNDLDLIRQVKELVAPGAKGIDAGCGPLAREVFCFYEDGYDMYGMD
                     VVPENIQTALEYHPSLADRLFLADLTQPLDVPDHSFDFIICNTVIQHINLNSLTNVTFP
                     EWTRILKPGGIIQLMFRSGHGTQIIHDVEFNDQRSFYLYQPEEILEILARYGLSLIPEE
                     NGNLGGIIYVTDVRPMENCIFCVRKTD"
     gene            1018..1821
                     /gene="gntA"
                     /locus_tag="K2F26_16455"
     CDS             1018..1821
                     /codon_start=1
                     /gene="gntA"
                     /inference="COORDINATES: protein motif:HMM:NF020466.2"
                     /locus_tag="K2F26_16455"
                     /note="GntA, YqcI/YcgG family protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="heme dependent pre-guanitoxin N-hydroxylase"
                     /protein_id="QYX30481.1"
                     /transl_table=11
                     /translation="MRKNPFESNLALEHSCYAKFDGTEFKILESGTKISEKVTEVHAEF
                     REKILHRTPPFTCMGATSVFNQNNYRFSMYSDMCTKETTLALARNLFTFVNEQDSMNSH
                     FTAFIAVFEKPIPEDEHHFEKLVWSQLQMLHDEDSRYHDWDTQFSNDINNHKFSYSFAE
                     RSFFIIGMHPGSSRIARQFSYPLLVFNASRQFDHLVETNQFHNFVRIIRERDVALQGSI
                     NPNLPAKYDHSRPEEPEVRQYSGRAVEKDWQCPLVVHQKKHKHNQ"
     gene            1845..2801
                     /gene="gntB"
                     /locus_tag="K2F26_16460"
     CDS             1845..2801
                     /codon_start=1
                     /gene="gntB"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /locus_tag="K2F26_16460"
                     /note="GntB, fatty acid desaturase family protein; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="L-arginine gamma (S) hydroxylase"
                     /protein_id="QYX30482.1"
                     /transl_table=11
                     /translation="MKKNIKKYRFSNSIYHQIKPLLKLDQWHCWLALAEDWIVICFAIS
                     ISYYLSWYFYPVTILLIGSRQRALATLLHEAAHQVIAKNRTLNFMMGTFFSGYFILQTM
                     SSYRKSHVEKHHRYFGNPDEDPDYKFAISQGLYQQSLDDKTFQMLYIFSPLLLAQVPKY
                     IKSLLIHRFLEDQNYLELVAIGLWWLTIISLSILFNLWQYLILFWLIPYLTVFQIIGWF
                     IELAEHYPLMNNDINLYMSRNRNSHFIEKFLTGMHNENYHLVHHLFPNIPFWNVPKAHE
                     ILMQDPNYAQHHSQSGGIVWSNNNAPSILSGLTQVSS"
     gene            2953..4065
                     /gene="gntC"
                     /locus_tag="K2F26_16465"
     CDS             2953..4065
                     /NRPS_PKS="Domain: Aminotran_1_2 (53-363). E-value:
                     1.3e-40. Score: 131.4. Matches aSDomain:
                     nrpspksdomains_K2F26_16465_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="gntC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019:aminotransferase (Score: 206.8; E-value:
                     8.4e-63)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /locus_tag="K2F26_16465"
                     /note="GntC, aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="PLP-dependent (S)-gamma-hydroxy-L-arginine
                     cyclodehydratase"
                     /protein_id="QYX30483.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2.8e-41, bitscore:
                     131.3, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 5.9e-10, bitscore:
                     28.0, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.6e-08,
                     bitscore: 23.7, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIQPALLEMWLSEYERVPYNLGESSVDNFTLGELLNLTGDRDAL
                     DQLSLMNNDTHGSLRLREAIASLDKSVSPDDILVTAGTTEAILIYFKVRYRSGANVVVP
                     VPTFHVLYETPAFLGYEVRYLQLRAENGFRIDPQELAKLVDDNTEVIVLNTPQNPSGVV
                     CSETEIQSIIEIAEKHNAEILADEHYRFLPHDQDTEILPSLYGLSPKIISLGSTGKCFG
                     CIGLRIGWLIGNPEIIKACHFFKDYTTHTVCVLNDYIAAGVLLHKGKILPRYRQMIQHN
                     IQQFETFIKQQRGLIDWVKPEAGTIAFPFFTDPNINSKIVAKRLVEDHGVLLLPGEAFD
                     RPSHFRIALGVEPSLFQYALEKLAIVIETS"
     aSDomain        3112..4041
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K2F26_16465_Aminotran_1_2.1"
                     /evalue="1.30E-40"
                     /label="K2F26_16465_Aminotran_1_2.1"
                     /locus_tag="K2F26_16465"
                     /protein_end="363"
                     /protein_start="53"
                     /score="131.4"
                     /tool="antismash"
                     /translation="DTHGSLRLREAIASLDKSVSPDDILVTAGTTEAILIYFKVRYRSG
                     ANVVVPVPTFHVLYETPAFLGYEVRYLQLRAENGFRIDPQELAKLVDDNTEVIVLNTPQ
                     NPSGVVCSETEIQSIIEIAEKHNAEILADEHYRFLPHDQDTEILPSLYGLSPKIISLGS
                     TGKCFGCIGLRIGWLIGNPEIIKACHFFKDYTTHTVCVLNDYIAAGVLLHKGKILPRYR
                     QMIQHNIQQFETFIKQQRGLIDWVKPEAGTIAFPFFTDPNINSKIVAKRLVEDHGVLLL
                     PGEAFDRPSHFRIALGVEPSLFQYALEKL"
     gene            4062..5105
                     /gene="gntD"
                     /locus_tag="K2F26_16470"
     CDS             4062..5105
                     /NRPS_PKS="Domain: TauD (110-321). E-value: 1.6e-17. Score:
                     55.9. Matches aSDomain: nrpspksdomains_K2F26_16470_TauD.1"
                     /codon_start=1
                     /gene="gntD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TauD"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /locus_tag="K2F26_16470"
                     /note="GntD, TauD/TfdA family alpha-ketoglutarate iron (II)
                     dioxygenase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /product="L-enduracididine beta-hydroxylase"
                     /protein_id="QYX30484.1"
                     /sec_met_domain="TauD (E-value: 3.2e-18, bitscore: 55.9,
                     seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMSIQTLNFTQSELEEIDILLDQVTANYSHAEQPELIDTAAVLAQ
                     SLPLRLRQFLHEFKLRETAPACLIKGYPVNDNTIGATPEHWAGHEDAKRTKREQIVFVL
                     CSALLGEIFGWLTQQNAYLIHDVFPIKGYEQDQIGSGSEALLLWHTEDAYHPARGDYLG
                     LMCLRNPCQSITTIGSVDSLKKLTQQQLDILFQPRFIIRPDNAHHADNNYQTETDLDTH
                     LQNAYNQIKHKIISQDNAMALLYGEPSAPYLCVDPAYMEATDEEAKQTLQALIDAIDIE
                     IMDVILQPGDICFVDNFRAVHGRKPFKANYDGNDRWLKRINITRDLRKSKTYRSHPTSR
                     IIDKVPS"
     aSDomain        4392..5024
                     /aSDomain="TauD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K2F26_16470_TauD.1"
                     /evalue="1.60E-17"
                     /label="K2F26_16470_TauD.1"
                     /locus_tag="K2F26_16470"
                     /protein_end="321"
                     /protein_start="110"
                     /score="55.9"
                     /tool="antismash"
                     /translation="EIFGWLTQQNAYLIHDVFPIKGYEQDQIGSGSEALLLWHTEDAYH
                     PARGDYLGLMCLRNPCQSITTIGSVDSLKKLTQQQLDILFQPRFIIRPDNAHHADNNYQ
                     TETDLDTHLQNAYNQIKHKIISQDNAMALLYGEPSAPYLCVDPAYMEATDEEAKQTLQA
                     LIDAIDIEIMDVILQPGDICFVDNFRAVHGRKPFKANYDGNDRWLKRI"
     gene            5120..6430
                     /gene="gntE"
                     /locus_tag="K2F26_16475"
     CDS             5120..6430
                     /NRPS_PKS="Domain: Aminotran_3 (35-364). E-value: 3.2e-65.
                     Score: 212.2. Matches aSDomain:
                     nrpspksdomains_K2F26_16475_Aminotran_3.1"
                     /codon_start=1
                     /gene="gntE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 303.4;
                     E-value: 4.2e-92)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /locus_tag="K2F26_16475"
                     /note="GntE, PLP-dependent aspartate aminotransferase
                     family protein; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /product="PLP-dependent transaminase"
                     /protein_id="QYX30485.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.6e-74, bitscore:
                     239.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKDSPKSHAFFERAQKVIPFGVNSSHRYWGENTPVIQRGEGAYV
                     YDFDGKSYIDYRLGFGPVILGHAHPFVNQRVIAAIQNGVSFSATQEYEVRVAEHIIKMC
                     PSVDMVRLDNSGSDATRHAIRLARGYTGRDLVLKFEGAYHGDYDYMLWTTPDSLKSRVG
                     KRGEPIGHKTSQGVPDLVSQLLLIAVWNDREGIEKLLKEKGDSIAAIIVEPILANAGGL
                     MPEPGFLQFLRNQCDIYGIVLIFDEVKTGFRIAPGGAGEYFGVQADLSAYAKAMGNGYP
                     ISAIGGKRDIMMTIAPGKVVHAGTYTGNVVVTAAADATLEYMQQQDVFGHLNHVGQQLM
                     DGLDEILTRHQINHHIHGLPSLFGLTLSESNPKDWRDVVDTNLELSEQLLIELVNAGIM
                     TDSDPQQTWYVCESHTSEDIAETLNRFETALRKALQK"
     aSDomain        5225..6211
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K2F26_16475_Aminotran_3.1"
                     /evalue="3.20E-65"
                     /label="K2F26_16475_Aminotran_3.1"
                     /locus_tag="K2F26_16475"
                     /protein_end="364"
                     /protein_start="35"
                     /score="212.2"
                     /tool="antismash"
                     /translation="VIQRGEGAYVYDFDGKSYIDYRLGFGPVILGHAHPFVNQRVIAAI
                     QNGVSFSATQEYEVRVAEHIIKMCPSVDMVRLDNSGSDATRHAIRLARGYTGRDLVLKF
                     EGAYHGDYDYMLWTTPDSLKSRVGKRGEPIGHKTSQGVPDLVSQLLLIAVWNDREGIEK
                     LLKEKGDSIAAIIVEPILANAGGLMPEPGFLQFLRNQCDIYGIVLIFDEVKTGFRIAPG
                     GAGEYFGVQADLSAYAKAMGNGYPISAIGGKRDIMMTIAPGKVVHAGTYTGNVVVTAAA
                     DATLEYMQQQDVFGHLNHVGQQLMDGLDEILTRHQINHHIHGLPSLFG"
     gene            6634..7458
                     /gene="gntF"
                     /locus_tag="K2F26_16480"
     CDS             6634..7458
                     /codon_start=1
                     /gene="gntF"
                     /inference="COORDINATES: protein motif:HMM:NF013405.2"
                     /locus_tag="K2F26_16480"
                     /note="GntF, methyltransferase domain-containing protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="pre-guanitoxin forming N-methyltransferase"
                     /protein_id="QYX30486.1"
                     /transl_table=11
                     /translation="MELKFQENDFQSFEPIPYLNEYFNYPSDYYGGVGFENEQFLQFFA
                     EVAHEYHLNNYLLLDFGCGPTIYSILSLGRNCREIHMSDYLQQNLEQVQLWQQGKPQAF
                     DWYPYLRRALQMETALNQNQPLDSFLDVTDEEIEERARLLREKITSIRKGNARATNPVG
                     EEGKALYEAVVSSFCLEGVAQDLAEWKNLITNLSSTIKPGGLLIFATQIEADSYRIGEG
                     YGTVVNLTEQDIVQTLLNCNFESGSIKTKELKGGPNHTEYDKFLMLTARLAV"
     gene            7464..8498
                     /gene="gntG"
                     /locus_tag="K2F26_16485"
     CDS             7464..8498
                     /EC_number="4.1.2.48"
                     /NRPS_PKS="Domain: Beta_elim_lyase (7-289). E-value:
                     1.9e-92. Score: 301.4. Matches aSDomain:
                     nrpspksdomains_K2F26_16485_Beta_elim_lyase.1"
                     /codon_start=1
                     /gene="gntG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF007825.0"
                     /locus_tag="K2F26_16485"
                     /note="GntG, low-specificity L-threonine aldolase; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="PLP-dependent aldolase"
                     /protein_id="QYX30487.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.1e-09, bitscore:
                     27.5, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 2.6e-08,
                     bitscore: 23.0, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEHFPIDLRSDTVTQPTPSMRQAMAEAIVGDDVYGEDPTVNRLE
                     RVAAELLGKESAMFVPSGTMGNLICMLTHCGRGEEVIIGDQAHLFLYERGGSAVLGGIH
                     PYTVSTATDGTLPLSEIRAAVRPDDIHCPRTKLLCVENTQAQRGGLPLSLTYLHSLRDL
                     ANELGLRLHCDGARLWNASVAIGVEPSVLSSPFDSLSVCLSKGLAAPIGSLVIGTQSFI
                     DEARRLRKLLGGGMRQVGAIASAGLIALEQMRERLAIDHENAQFLAEGLRECGYEITHP
                     VRTNIIFFRQHEQDTLSLQERLAVWKNSGLLVSDFGDRTIRAVTHYGIERKDINQAISI
                     LQQV"
     aSDomain        7485..8330
                     /aSDomain="Beta_elim_lyase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K2F26_16485_Beta_elim_lyase.1"
                     /evalue="1.90E-92"
                     /label="K2F26_16485_Beta_elim_lyase.1"
                     /locus_tag="K2F26_16485"
                     /protein_end="289"
                     /protein_start="7"
                     /score="301.4"
                     /tool="antismash"
                     /translation="DLRSDTVTQPTPSMRQAMAEAIVGDDVYGEDPTVNRLERVAAELL
                     GKESAMFVPSGTMGNLICMLTHCGRGEEVIIGDQAHLFLYERGGSAVLGGIHPYTVSTA
                     TDGTLPLSEIRAAVRPDDIHCPRTKLLCVENTQAQRGGLPLSLTYLHSLRDLANELGLR
                     LHCDGARLWNASVAIGVEPSVLSSPFDSLSVCLSKGLAAPIGSLVIGTQSFIDEARRLR
                     KLLGGGMRQVGAIASAGLIALEQMRERLAIDHENAQFLAEGLRECGYEITHPVRTNIIF
                     F"
     gene            8690..9931
                     /gene="gntH"
                     /locus_tag="K2F26_16490"
     CDS             8690..9931
                     /codon_start=1
                     /gene="gntH"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /locus_tag="K2F26_16490"
                     /note="GntH, candidate phosphatase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="QYX30488.1"
                     /transl_table=11
                     /translation="MSNNKQDINHTELNPMAHSRRGFFTVFIAGISTSVSSIFRKDVAV
                     AENHKAFTAENKSMLSTNNQSFFFPGELLASDEMRITIVGSSSVARKTQSGTSIFLELG
                     NGDSFIFDAGPGVAANYTALGVPYSRMDRIFLSHLHCDHITDLAYIYGQGSMYDRKTPL
                     RIWGPSGKESKFGTNAFVESMKAMCLWHTESLSYHQNGNGYETEVHEFDYAQNPGVAYN
                     EKGVVIKHFPAVHLKDGAVSYRVDWNGLSFVFSGDSQPNTFMVENAQNVDILIHQTIPP
                     AQVWAKEYNMPLEQVIELQNRFDSPPHAVGAIFDQTRPRLGVLTHLYLNENIKVAILDS
                     CQEVYDGPIEIAQDLMVFNISKNEIKQRIAIVPELALQNTVKKYENLPPPKYKPREQLS
                     QWLLDAVIPQEQWK"
     gene            9994..10911
                     /gene="gntI"
                     /locus_tag="K2F26_16495"
     CDS             9994..10911
                     /codon_start=1
                     /gene="gntI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /locus_tag="K2F26_16495"
                     /note="GntI, phosphotransferase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="pre-guanitoxin N-oxide kinase"
                     /protein_id="QYX30489.1"
                     /sec_met_domain="APH (E-value: 4.5e-21, bitscore: 65.4,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKSIDKIITNLPLWDTQGALKIVPIGDQNFTNQNYRVESNGQVF
                     KVRISEGNQNLIGIAKEEELIVLKAVAKMGIGPEVIAYIPPEGHLVTRFIEGKHFSLQE
                     ITQPENIHRIAQILKQVHSIEGIDAAPPPFERIEGLMRNAERKNGIFPDDFEQLLADLQ
                     AIKAVLTKVHRKPCLCHNDLAGSNIIEADGSIFLIDWEYAGVANPMFDLANFSMNQNLD
                     REGDKILIESYFGKVSQVELAEINLWKIVAIFIEGVWGVLQAKISQFDRDYQGFANENW
                     QTVRQYTQKESFSEWLSMISLSNH"
     gene            11219..12577
                     /gene="gntT"
                     /locus_tag="K2F26_16500"
     CDS             11219..12577
                     /codon_start=1
                     /gene="gntT"
                     /gene_functions="transport (smcogs) SMCOG1086:MATE efflux
                     family protein (Score: 242.2; E-value: 1.5e-73)"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015078138.1"
                     /locus_tag="K2F26_16500"
                     /note="GntT, MATE family efflux transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="MATE family efflux transporter"
                     /protein_id="QYX30490.1"
                     /transl_table=11
                     /translation="MYLTLFKQYDFLNRFIRLSVANIVSNLMIPLAGSISLVFLGHLSG
                     VHDLAGSSFAVVLFDFIYYGFYFLRQSTTGVTAQAIGREDQEEMLLIGLRNILIALVLG
                     ILLLILQYPFREIAFSMLNDTPEVKASAVAYFNARIIGAPAVFINYVLIGCLLGREESG
                     KVLLISVMGNAVNILLDYLLIIHWGWGATGAGLSMAISQYLMLLVGLIFFCLQIQWQML
                     KAITGKLLDWSALKSAFVLNGNIFVSTLILISTTDIFSLESNTMGTIIFTQNTLILQII
                     FLALYFVEGLGLATESLVGYFTGQGAKEKLASLVGLSAQASLVLGLAFALVPALFPQWV
                     FGLFTDHIEIISQIDTYIWWLPLFLTFSSVALALEGYFLGLAEGRTLRNINLTAIILGF
                     MPLSIAAWQFHNNHLLWLAYSMFMCIRMVMLSIQVPRTFESDFGERKILGEME"
ORIGIN
        1 atcttttaat ctgtcttccg cacacagaaa atacaatttt ccattggtct gacatcagta
       61 acataaataa ttcctcctaa attgccattc tcttctggaa ttaaagacaa tccatatctc
      121 gccagtattt ctagaatttc ctctggttga tacagataaa aactacgttg atcgttaaat
      181 tctacatcat gaataatttg agttccatga ccagacctaa acattaactg aatgattcca
      241 ccaggcttaa gtattcgtgt ccactctgga aaggttacat tagttaaaga attgagatta
      301 atgtgttgaa ttactgtatt gcaaataata aaatcaaagg aatgatcagg gacatcaagt
      361 ggctgcgtca aatcagccaa aaaaagccga tcagccaatg aaggatgata ctccagtgcc
      421 gtttggatat tttctggaac tacatccata ccatacatat cataaccatc ttcataaaag
      481 cagaatacct cccgggcaag aggaccacaa ccagcatcaa ttccttttgc accaggagca
      541 acaagttctt ttacttgacg aatgaggtcg agatcatttt tgaagccagg atgagaaata
      601 ttcgtgtatt ctgaccaaga ttcgttgaga atcttagcaa atactcctgc ttgtcttgca
      661 taaaaatcac gtgcttctgc ctgaatgtga tctgaactta aatcagtgga attataacct
      721 acaacattac gacttaaaat atttgatttt accattttat tacaccttga aagtagttta
      781 tatgcagtaa tgaagcatct ataggaatct ggttttgttt ctaatcaaag ggagcataac
      841 attctatact agtcctttag ataattctta attttttctt aaacattgcc taataattgg
      901 ttaaatttta ttaaaacccc ctgattatgc tttttttgct gtataaacag agctgtataa
      961 acagaaaagt taagctcatt ccccggctca acgataaaaa aaatcttata gattgatatg
     1021 cgaaaaaatc cttttgaaag taatctagca ctggaacaca gttgttatgc caagtttgat
     1081 ggaacagaat ttaaaatact tgagagtggt acaaaaatat cagagaaggt aactgaagta
     1141 cacgctgaat ttcgggagaa aatattgcat cgcactcctc cttttacttg tatgggtgca
     1201 acatccgtat ttaaccagaa taactatcgt ttcagtatgt acagtgatat gtgtacaaaa
     1261 gaaactacct tagcattggc tcgaaattta ttcacttttg tgaatgaaca agatagcatg
     1321 aatagtcact tcacagcatt tattgctgtg tttgaaaaac caataccaga agatgaacac
     1381 cactttgaaa aactggtgtg gagtcaacta caaatgttac atgatgaaga tagccgttac
     1441 catgattggg acacccaatt cagcaacgat atcaataatc ataaattttc atacagtttt
     1501 gctgaaagaa gctttttcat tattggaatg caccctggaa gttctcgaat tgctaggcaa
     1561 ttttcctacc cacttctagt atttaatgcc agtcgtcaat ttgatcattt agttgaaact
     1621 aaccaattcc ataacttcgt cagaattatt cgggagcgtg acgtagctct gcaaggtagt
     1681 attaatccta atctgccagc aaaatatgac catagtcgtc ctgaagaacc tgaagtcaga
     1741 caatattctg gacgtgcagt agaaaaagat tggcaatgtc ctttagttgt acaccagaag
     1801 aaacataagc ataatcaata atgaatcagt gaagttttaa tgccatgaaa aaaaatatta
     1861 aaaaatatcg cttcagcaat tctatttatc atcaaataaa accgctttta aaacttgatc
     1921 agtggcattg ttggttggct ttagcagaag actggatagt tatttgtttt gctatttcca
     1981 ttagctatta tttgtcttgg tacttttatc ctgttaccat actactcatc ggttcacgtc
     2041 agagagcatt agccacattg ctccatgaag cagcccatca agtaattgcc aaaaatcgca
     2101 ccttaaactt tatgatgggt acttttttct caggatattt cattctgcaa acaatgtcat
     2161 cttatcggaa atctcatgtt gaaaaacatc atagatattt tggcaatccg gacgaagatc
     2221 ctgactacaa gtttgcaatt tctcaaggat tatatcaaca gagtttggat gataaaacct
     2281 ttcagatgct ttatattttt tctcctttgc tacttgctca agtacctaaa tatataaaat
     2341 ctttattaat tcaccgtttc ctagaagatc aaaattatct agaactggtt gctattggat
     2401 tgtggtggct aacaattatt agcctcagca ttttatttaa cctatggcaa tatttaattc
     2461 tcttctggtt aattccttat ttgacggttt ttcaaatcat tggctggttt attgaattag
     2521 ccgagcatta ccctttaatg aataatgata tcaatttgta tatgagtcgt aaccgcaaca
     2581 gccattttat tgagaaattt ctaacaggta tgcataatga aaactaccat ttagtacatc
     2641 acctatttcc caatattcct ttctggaatg tgcctaaagc acacgaaata ttgatgcaag
     2701 atccaaatta tgcccaacat cacagtcaaa gtggaggaat agtctggtca aataataatg
     2761 cacctagtat tttatcagga cttacacaag tgtcatcata aatatctatt gtagggtgcg
     2821 tcagatatca ataatctatt tatttgcttg atttatggaa tctgacgcac cctaccaatg
     2881 tgctttgttg cgtaagtcct atttatgtct caagtctgaa cataggtgta caattccaat
     2941 gggggaaaat aaatgaaaat tcaacctgct ttattagaaa tgtggctgtc tgaatatgaa
     3001 cgagtacctt ataacttagg agaaagcagt gttgataatt ttaccttggg agaattacta
     3061 aatcttacag gagatcgtga tgctcttgat caactcagcc tcatgaataa cgacacccac
     3121 ggctccctga gattacgtga agcgatcgcc tcattagata aatcagtatc cccagatgat
     3181 attttagtga ctgctgggac aacagaagcc atattaattt attttaaagt acgttaccgt
     3241 tccggtgcta atgtcgttgt tccagtacca accttccatg tactttatga aactccagct
     3301 tttttaggtt acgaggtacg ctatctgcaa ttacgagcag aaaatggttt tcgcattgat
     3361 cctcaagaat tagccaagtt agttgatgat aatactgaag taatagtttt gaatactcca
     3421 caaaatccta gtggtgttgt ttgctcagaa acagaaattc agtccataat tgagattgca
     3481 gaaaagcata atgcagaaat tttagctgat gagcattacc gttttttacc ccatgatcaa
     3541 gacacagaaa ttctgccttc gttgtatgga ctttcaccca aaatcatcag tttaggttct
     3601 acaggtaaat gctttggttg tattgggtta cgcattggtt ggctgattgg caacccagaa
     3661 ataattaaag cttgtcactt tttcaaagac tacacaaccc acactgtctg cgtccttaat
     3721 gattacatcg ctgctggagt cttattacac aaaggcaaaa ttttaccccg ttatcgtcag
     3781 atgattcagc acaatattca gcagtttgaa acttttatca aacaacaacg gggattaatt
     3841 gactgggtta aacctgaagc gggtactatc gcttttccgt tttttaccga tccaaatatc
     3901 aacagcaaaa tagtagccaa acgtctggta gaagatcatg gagtgttgtt gctacctggt
     3961 gaagcatttg atcgccccag tcatttccgc atagctttgg gtgttgaacc aagccttttt
     4021 cagtacgcat tggaaaaact tgctattgtg atagaaactt catgatgagt attcaaacgt
     4081 taaattttac tcaatctgaa cttgaagaaa tagacattct tttagatcaa gttactgcta
     4141 actatagcca tgcagaacaa ccggaattga ttgacactgc tgcggtatta gcccaaagtt
     4201 tgcccctacg cctgcgtcaa tttttacatg agttcaaatt gcgagaaacc gctcctgctt
     4261 gtctcatcaa gggttatcca gttaatgata atactattgg cgctactcct gaacattggg
     4321 caggtcatga agatgccaag cgcactaaac gagagcaaat tgtgtttgtt ttgtgcagtg
     4381 ctttactggg agaaatattt ggttggctaa cacagcaaaa tgcctatctg atccacgatg
     4441 tttttccaat taaaggctat gaacaggatc aaattggttc tggtagtgaa gcactactgc
     4501 tttggcatac tgaagatgct tatcatcctg ctcgtggtga ctacttgggg ctaatgtgtt
     4561 tgcgtaatcc ttgccagagt ataaccacta ttggttcagt ggattcatta aaaaaactga
     4621 ctcagcagca attagatatc ttgtttcaac cacgttttat tattcgccca gataatgccc
     4681 atcatgccga taataattat caaacagaaa ctgatttaga tactcatctt cagaatgctt
     4741 acaatcaaat aaagcataaa attatctccc aagataatgc tatggctttg ctatatggag
     4801 aacctagcgc accctatttg tgtgttgatc cagcttatat ggaagcaaca gatgaagaag
     4861 ctaaacagac tttgcaagcc ttgattgatg ctattgacat tgaaatcatg gatgtaattt
     4921 tacaacccgg tgatatttgt ttcgtagata atttccgtgc tgtgcatggg agaaaaccat
     4981 ttaaagccaa ttatgatggt aatgatcggt ggttaaagcg aattaatatc acacgcgatc
     5041 ttcgcaaatc taaaacatat cgttctcatc ctacatctcg aattattgat aaagtacctt
     5101 cataaatcct tcataaatta tgttaaaaga ttccccaaaa tcccatgctt tctttgaacg
     5161 cgcacaaaaa gttatcccct ttggtgttaa ttccagccac cgctactggg gcgaaaatac
     5221 acctgtaatt cagcgtggtg aaggagcgta tgtttatgac tttgacggaa aaagctatat
     5281 tgattaccgt ctaggttttg gtccagttat tcttggtcat gctcatcctt ttgttaatca
     5341 gcgtgtcatt gcagcaatac aaaatggtgt aagtttttct gcaactcaag aatatgaagt
     5401 ccgggtagct gaacatatca tcaagatgtg tccttctgtt gacatggtac gtctggataa
     5461 tagtggttct gatgctaccc gccatgcaat tcgtctggcc agaggttaca ctggacgaga
     5521 tctagtgttg aaatttgaag gtgcttatca cggtgattac gattatatgc tttggacaac
     5581 accagatagt cttaaaagcc gtgtgggaaa acgaggcgag ccaattggac ataaaaccag
     5641 tcaaggtgta ccagacttag tatctcaatt gttattaata gcagtttgga atgatcgtga
     5701 gggaatagaa aaactgttaa aggaaaaagg agatagtatt gctgccatta ttgtcgagcc
     5761 gattcttgct aatgcaggtg gcttgatgcc ggaacctgga tttttgcaat tcctccgtaa
     5821 tcaatgtgat atttatggta ttgttttaat tttcgatgaa gttaaaactg gttttcgcat
     5881 cgcaccagga ggagcaggtg aatattttgg cgtacaggct gacttgagtg cctatgctaa
     5941 agctatgggt aatggctatc cgatttctgc tattggtggc aagcgtgaca ttatgatgac
     6001 catagcacca ggtaaggtgg tacatgcagg aacttataca ggaaatgtag tagttactgc
     6061 tgcggccgat gctacccttg aatatatgca acagcaggat gtatttgggc atctcaacca
     6121 cgttggacaa caactgatgg acggtttaga tgaaattctg actcgccatc aaattaatca
     6181 tcatattcat ggtttaccct cattgtttgg tttaactttg tcagaatcta atcccaaaga
     6241 ttggcgtgat gttgttgata ccaatttgga attatctgaa caactactga ttgaattggt
     6301 caatgctggt attatgactg attctgatcc acaacaaact tggtatgtgt gtgagtctca
     6361 cacgtctgaa gatattgcag aaacactaaa taggtttgaa actgccctgc gtaaagcttt
     6421 acagaaataa aaaacttcat tatctgtaat ttaatggcta atttacagca gttttcgtta
     6481 gcgtagcgtg acgacgtagg agtcattgtt atgaggtaca ctcatggcag gcaggcaaga
     6541 tgcctaccat acaatagttt tactattcat atctgtactt catttacctg caatctgcta
     6601 caagtgtatc taggttttaa aaaggagata aaaatggaac tcaagtttca agaaaatgat
     6661 tttcaatctt ttgaaccaat accttatctg aatgaatact ttaattatcc ttcagattat
     6721 tatgggggtg taggatttga gaatgaacaa ttcctacaat tctttgctga agttgcccat
     6781 gaatatcatc tcaataacta tttgctatta gatttcggtt gtggtcctac tatttattct
     6841 atcctcagct taggacggaa ttgccgtgaa atacatatga gtgattattt acaacaaaat
     6901 ttagagcagg ttcagctttg gcaacaagga aagccccaag ctttcgattg gtatccttat
     6961 ctgcgtcgcg ccttgcaaat ggaaactgcg ttaaatcaga atcagccttt agatagtttt
     7021 ttagatgtta cagatgaaga aatagaagaa cgggcaagat tgctacggga aaaaataact
     7081 tctatcagaa aaggcaatgc tcgtgctact aatcctgtgg gagaagaagg aaaggcatta
     7141 tatgaagcgg tggtttcttc tttctgttta gaaggtgtag cacaagattt agctgaatgg
     7201 aaaaatttga taactaattt gtcttccaca attaaaccag ggggtttatt gatatttgct
     7261 actcaaattg aagctgatag ttatcgaatt ggagagggtt acggcacagt ggttaatctc
     7321 actgaacaag atattgtgca gacattattg aattgtaatt tcgagtcagg ttcaatcaaa
     7381 acaaaggaac ttaaaggcgg tccgaatcac actgagtacg ataaattcct gatgttgaca
     7441 gccagacttg ctgtgtgaaa actatgagtg agcattttcc cattgattta cgcagtgata
     7501 cagttaccca accgacacca tctatgcgtc aagctatggc tgaagcgata gtaggtgacg
     7561 atgtttatgg agaagatcca acggttaacc gactggaacg agtagcagca gaacttttag
     7621 gtaaagagtc tgctatgttt gtacctagtg gcacgatggg taatctcatc tgtatgttaa
     7681 ctcattgtgg acgtggagaa gaagtgatta ttggtgatca agctcatctg tttttgtatg
     7741 aacgtggtgg cagtgctgta ttggggggta ttcatcctta cactgtcagc acagcaacag
     7801 atggtactct tcctttatca gaaatccgcg ctgctgtaag acctgatgat attcattgtc
     7861 cgagaacaaa gctattatgt gtggaaaata cacaagcaca acgaggcggt ttgcctttat
     7921 cattaactta tttacacagc ttgcgtgatt tggcgaatga gttaggatta agattgcact
     7981 gtgatggagc gcgtttgtgg aacgcatctg tggcaattgg ggttgaacct agtgttttat
     8041 cttcaccttt tgattcttta tctgtctgtt tatcaaaggg tctggctgca cctattggtt
     8101 ctcttgtgat cggaacacag tcatttattg atgaagcacg gcggttacgt aaactattgg
     8161 gtggtggtat gcgtcaagtt ggagcgatcg cctctgctgg tttaattgcc ctagaacaaa
     8221 tgagggaaag actggctatt gatcatgaaa acgcccaatt tttagctgaa ggtttacgtg
     8281 aatgtggtta tgaaatcact catcctgtgc ggactaatat catatttttt cgccagcatg
     8341 agcaagatac cttatctctc caggaaagat tagctgtttg gaaaaactct ggcttgttag
     8401 tctcagattt tggcgatcgc acaattcgcg cagttaccca ttatggtatt gaacgcaaag
     8461 atattaatca agcaatttct attttacaac aagtctagtc tggtataaaa attaggactt
     8521 acgtaaaagt accaaatcgg gaaagataca gcagtttctg gtattatgag gtacagatat
     8581 taatgaaaaa acccttatag tgcgggaggg acagcccgaa tctgtacctc aggcagatgg
     8641 aatgtgctgt aaatgatgtg aacagaacaa gccaatattt gtaatcttca tgagtaacaa
     8701 caaacaagac atcaatcaca cagaattaaa tcccatggct catagccgca ggggattttt
     8761 tactgtgttt attgcaggaa tttctacatc tgtatctagt attttcagga aagatgttgc
     8821 tgtagctgag aaccataaag ccttcaccgc cgaaaacaaa tcaatgctct ctaccaataa
     8881 ccagtcattt ttttttccag gtgaattgtt agcatctgac gaaatgcgga tcactattgt
     8941 agggtcttct tccgtcgcac gcaaaaccca atctggaaca agcatctttc tcgaactggg
     9001 aaacggggac agtttcatct ttgatgccgg acctggtgta gctgccaact atacagcgtt
     9061 gggtgtaccc tattcgcgga tggatagaat tttcctaagt caccttcatt gcgatcacat
     9121 aacggatctt gcctacatat atggtcaagg atcaatgtat gatcgcaaaa cacccctacg
     9181 tatctgggga ccgagtggca aggagtcgaa gtttggcaca aatgctttcg tcgagtcaat
     9241 gaaggctatg tgtctctggc acacggagag tctttcctac caccagaacg gtaatggtta
     9301 tgaaacagag gtacatgaat ttgactatgc tcaaaatcca ggtgttgcat acaacgagaa
     9361 aggagttgtg attaagcact ttcccgcagt tcacctgaaa gacggtgcgg taagctaccg
     9421 tgtggattgg aatggtctca gttttgtctt ctctggcgac tcgcaaccga atacatttat
     9481 ggttgagaac gcacaaaatg ttgatatctt gattcaccaa acaattcctc cagcacaggt
     9541 ttgggcaaaa gaatacaata tgcccctcga acaagttata gaactgcaaa acagatttga
     9601 ttctcctccc catgctgttg gtgcgatttt cgaccaaact agaccacgct tgggtgtact
     9661 aactcattta tatctaaatg aaaatataaa agttgccatt cttgactcgt gccaagaggt
     9721 ttacgacgga cctattgaaa ttgctcagga tttgatggtt ttcaatatct ccaagaatga
     9781 aatcaagcag cgcatagcga tcgtccctga attagcgttg caaaacaccg tcaagaagta
     9841 cgaaaacctt ccaccaccta agtataaacc aagggagcag ttgtcccagt ggcttctgga
     9901 cgcggtaatt ccgcaagaac aatggaaata gaatttgtat cagcttgatg agtatgtaga
     9961 acacaagtaa aagtaagatt aaggtagcaa aatttgaaaa aatccatcga caagatcatt
    10021 accaacttac cgctatggga tacacaaggt gctctgaaaa ttgtgccaat cggtgaccaa
    10081 aacttcacca atcagaatta ccgagttgag agcaacggac aggtattcaa ggttcgcatc
    10141 agtgaaggaa atcaaaacct catagggatt gccaaagaag aagaactgat tgtgcttaag
    10201 gcagttgcta aaatgggtat aggaccagag gtcattgcat acataccacc agaaggtcat
    10261 ctggtaacgc gatttataga gggtaagcat ttttctctac aggaaatcac gcaaccggaa
    10321 aacatacatc gaattgccca gatcctaaag caagttcata gtattgaggg gattgacgct
    10381 gcaccgccac catttgagcg aattgagggg ctgatgagga acgctgaacg caaaaatggc
    10441 atatttcctg atgattttga acagttactc gctgacctac aagctataaa ggctgtactg
    10501 actaaagtcc acagaaaacc atgtctgtgt cataacgact tggctggctc gaatatcatt
    10561 gaggccgatg gttcaatttt cctgatcgat tgggagtacg caggtgtagc aaatccgatg
    10621 tttgacctag ctaacttttc tatgaatcag aacctggata gagaaggtga taaaattctc
    10681 attgagagct actttgggaa agttagtcag gtggagttag cagaaatcaa tctttggaag
    10741 atagttgcta tttttattga aggtgtttgg ggtgttttgc aggcaaaaat ttctcaattt
    10801 gatcgggact atcaaggttt tgctaacgaa aattggcaaa cagtgcgtca gtacacgcaa
    10861 aaggaaagct tttcagaatg gctgtccatg atatcgttat caaaccactg atgaagtggg
    10921 aaattactgg gagggtgaca aaaatcttta aaccaattat accgcaaggg ttttgaacct
    10981 tcgtattctg agaaaatcaa catcattttg tgtatttatt gatgccattc tcaataacca
    11041 aaacccacta gcctgatttt tgtattggga caggtttcca atttgtttga atcttttcca
    11101 agctgaccca tttacagtcc ttattttata agggttttag tcgaagttgt cacctcttca
    11161 gaggaaatta ttaatccctg gatctgactt tttcatccat tctttatcac tgaatttaat
    11221 gtatttgaca ctcttcaaac agtatgactt tctgaaccga tttattcggt tatcggttgc
    11281 caatatagtc tccaatctca tgatccctct agctggttcc atcagtctag tttttttggg
    11341 tcatctctca ggagtccatg atttggccgg atcatctttt gcagtagtat tatttgattt
    11401 tatctactac ggtttttact ttttacgaca gagtaccact ggcgttaccg ctcaggcaat
    11461 aggaagagaa gaccaggagg agatgttact gataggactg cgaaatatct taattgcttt
    11521 agtcttaggc atactgcttt tgattttgca gtacccattt agggagatag cattttccat
    11581 gttaaatgat actccagagg ttaaagcttc ggcggttgcc tatttcaatg ctcggattat
    11641 tggtgcgcct gcggttttca tcaattacgt tctgattggt tgtttgctag gaagggaaga
    11701 gagcggtaag gtgttactaa tatcagtaat gggaaatgct gtcaacatat tactagacta
    11761 tttgctgatt atccattggg gttggggagc tacaggagct ggactctcta tggctattag
    11821 tcagtattta atgttattgg taggattaat atttttttgc cttcaaatcc agtggcaaat
    11881 gttaaaagct ataactggaa aacttttgga ttggtcagct ttgaaatctg cctttgtcct
    11941 gaatggcaat atatttgtta gtaccttgat cctgatctcc accactgata tattcagttt
    12001 agagagcaat acaatgggga caatcatctt tactcaaaat accttgatat tacaaatcat
    12061 tttcctagct ctctattttg ttgaaggatt aggattagct acagagagtt tagtaggcta
    12121 ctttacaggt caaggtgcga aggaaaagtt agcttctttg gtagggcttt ctgcacaagc
    12181 tagtcttgtt ttgggactcg cctttgctct tgtacctgcg ctttttcctc aatgggtatt
    12241 tgggttattc accgaccata ttgaaataat tagtcaaatt gatacatata tttggtggtt
    12301 accacttttt ttgacattta gttcagtagc acttgcactg gaaggatact ttctaggttt
    12361 agcagaagga cgcactcttc gcaatattaa cttaactgcc attattttag gatttatgcc
    12421 tttaagtata gccgcttggc agtttcacaa taaccatttg ttgtggcttg cttattctat
    12481 gtttatgtgc atcagaatgg tgatgcttag tatacaagta ccaagaactt ttgaaagtga
    12541 ttttggagag cgaaaaattt tgggggagat ggagtagata at
//
