BGC0002169 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002169 (Aspergillus aculeatus ATCC 16872)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	79,159	High	acurin A	PKS+NRPS:Type I

BGC0002169 - Region 1 - NRPS,T1PKS

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Location: 1 - 79,159 nt. (total: 79,159 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 79,160
Label		BGC0002169

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

ASPACDRAFT_122294: dioxygenase, TauD/TfdA

smcogs: SMCOG1121: dioxygenase, TauD/TfdA

ASPACDRAFT_1903457: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

ASPACDRAFT_45003: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

ASPACDRAFT_62004: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Transferases: sulfotransferase

ASPACDRAFT_61993: glutathione S-transferase

smcogs: SMCOG1193: glutathione S-transferase

Ligases: ligase synthase

ASPACDRAFT_122295: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

ASPACDRAFT_48: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002169.3	1.16	NRPS, PKS	acurin A	Aspergillus aculeatus ATCC 16872
BGC0001268.4	0.65	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.43	PKS	ACT-Toxin II	Alternaria alternata
BGC0002192.3	0.42	PKS	FR901512	fungal sp. No.14919
BGC0002261.2	0.35	PKS	lucilactaene	Fusarium sp.
BGC0002538.3	0.33	PKS	chaetolivacine A, chaetolivacine B, chaetolivacine C	Chaetomium olivaceum
BGC0001037.5	0.32	NRPS, PKS	pseurotin, azaspirene	Aspergillus fumigatus Af293
BGC0001026.3	0.31	NRPS, PKS	NG-391	Metarhizium anisopliae
BGC0002304.3	0.30	NRPS, PKS	varicidin A	Talaromyces variabilis
BGC0001273.3	0.30	PKS	asperlactone	Aspergillus ochraceus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002169.3	0.99	NRPS, PKS	acurin A	Aspergillus aculeatus ATCC 16872
BGC0002192.3	0.77	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.76	PKS	ACT-Toxin II	Alternaria alternata
BGC0001268.4	0.75	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002164.2	0.63	NRPS	peramine	Epichloe festucae
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 79159:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 47501:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 34500 - 79159:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ASPACDRAFT_48: pk

PKS_AT (540..859): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Ethylmalonyl-CoA: 62.5%; Methoxymalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 83.7
Methoxymalonyl-CoA: 69.8
Ethylmalonyl-CoA: 68.4
Malonyl-CoA: 40.5
Isobutyryl-CoA: 37.7
Propionyl-CoA: 33.5
Benzoyl-CoA: 30.4
fatty_acid: 28.6
trans-1,2-CPDA: 21.2
Acetyl-CoA: 18.0
3-Methylbutyryl-CoA: 16.6
CHC-CoA: 9.2
2-Methylbutyryl-CoA: 8.4
inactive: 0.0

ASPACDRAFT_122295: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (591..992): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DADQFGRVSK
Nearest Stachelhaus code(s):: Ser DADQFGRVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor