LOCUS       BGC0002174              3894 bp    DNA     linear   PLN 15-JAN-2020
DEFINITION  Epichloe festucae epls mRNA for e-poly-L-lysine synthetase, complete
            cds.
ACCESSION   BGC0002174
VERSION     BGC0002174
KEYWORDS    .
SOURCE      Epichloe festucae
  ORGANISM  Epichloe festucae
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Sordariomycetes; Hypocreomycetidae; Hypocreales; Clavicipitaceae;
            Epichloe.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:05:26
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..3894
                     /gene="epls"
     source          1..3894
                     /db_xref="taxon:35717"
                     /isolate="Fl1"
                     /mol_type="mRNA"
                     /organism="Epichloe festucae"
     protocluster    1..3894
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:3894]"
                     /cutoff="5000"
                     /detection_rule="(AMP-binding and TIGR02353)"
                     /neighbourhood="15000"
                     /product="NAPAA"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..3894
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(AMP-binding and TIGR02353)"
                     /neighbourhood="15000"
                     /product="NAPAA"
                     /protocluster_number="1"
     cand_cluster    1..3894
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(AMP-binding and TIGR02353)"
                     /kind="single"
                     /product="NAPAA"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..3894
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002174"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..3894
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NAPAA"
                     /region_number="1"
                     /rules="(AMP-binding and TIGR02353)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             1..3894
                     /NRPS_PKS="Domain: AMP-binding (29-401). E-value: 4.6e-73.
                     Score: 238.0. Matches aSDomain:
                     nrpspksdomains_epls_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (507-570). E-value: 8.8e-14. Score:
                     43.4. Matches aSDomain: nrpspksdomains_epls_PCP.1"
                     /NRPS_PKS="Domain: TIGR02353 (591-1271). E-value: 1.1e-161.
                     Score: 531.2. Matches aSDomain:
                     nrpspksdomains_epls_TIGR02353.1"
                     /codon_start=1
                     /gene="epls"
                     /gene_functions="biosynthetic (rule-based-clusters) NAPAA:
                     TIGR02353"
                     /gene_functions="biosynthetic (rule-based-clusters) NAPAA:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     233.4; E-value: 6.4e-71)"
                     /gene_kind="biosynthetic"
                     /product="e-poly-L-lysine synthetase"
                     /protein_id="BBU42014.1"
                     /sec_met_domain="AMP-binding (E-value: 5e-64, bitscore:
                     202.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.7e-14, bitscore:
                     39.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR02353 (E-value: 2e-163, bitscore:
                     531.2, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQPHSKDDLALYPVDDCGAPRTLVDILETTTAAHPKALAIDNGT
                     DRLTYLELAAQIAERVKQLRAAGVGAGDRIGVRVTSGSLELYVSILAVITAGASYVPVD
                     VDDPDERAQLVWGEANVTAVFTDNATLTNFNPPIGGEPRKPGLEDDAWIIFTSGSTGKP
                     KGVAVTHRSAAAFVDAESKIFLPNKPLAPGDRVLAGLSVAFDASCEEMWLAWRYGACLV
                     PAPRSLVKAGADLGAFLTAQSISVVSTVPTLAAMWPAEALQGLRLLILGGEACPPELAT
                     RLANTVDSVWNTYGPTEATVVACAAPLVAGQPVAIGLPLAGWKLAVVDAEGNTVRWGEE
                     GELVIGGVGMARYLDPEKDAVKFAPAACFGGERAYRSGDLVRAEKEGLFFVGRNDEQIK
                     LGGRRIELGEIDAALMTLPGVSAGATAIRRSETGNQVLVGYVVRTGAPQANDRGVLRRL
                     LPATLVPMIVTVDDIPVRTSGKVDRKALPWPPPASSHVDAQPIHGTTAWLAEQWRRVVG
                     VPASPDSDFFDIGGTSLAAAQLVSQLRQRAPTMSVADIYEYPILSAMAARVDDLAGTKQ
                     EDRMVRPTARWVMFVQACILICEITFNSGRWLAGVSAMKKFFALRLGPDSWAGVNPLPW
                     WLIAVIWALFMTFPGRLITTALGSRLLTWGIKPGTYRRGGGTHLRLWGAERFAALGNIG
                     AIAGTQWVRRYARLLGCHVGKDVQFHGLPPTTGLASFGDGCSVEPEVDTAGWWLDGDFL
                     HIGFITVGAGAVVGHRSTLMPGTVIEPYATVEGGTVVEGTVAGPDVTPSADEKAYMNER
                     SFFSGLRYTLTLLILDFLPIIPAAPTLALTPILVRDYSDFHNLVMTIIKVSAPGIVLGI
                     FLYVAMSVMFVRLASIFLRPGSYSWHSTAAWAAWITHGTMTQLRATMFPVFASLLTPTW
                     LRLFGARIGKYVEASTIIAIPSLLTVEDGAFLADDVVLSPFELCRGFIRVGPSSVGEKA
                     FVGNSGIVDPGVHVPERSLVGVLGTAPHDMEAGSSWLGRPAISLPRKQDIQVDDSLTFK
                     PPTRLVIFRGLIELCRLTPLLISGMLATGIATGYLWILNTFGYWQAILAGPAMMFSAGF
                     FALFLTTAVKWILTPRITAGMHRPLWSSFVWRNELADTFIQSLAVPWMIGSFYGTPLLV
                     WWMRTLGAKIGHGVWLDSHHLPEAELCEIGDGASVNRRAVLQTHLFHDRIMRLDKVVLE
                     AGATLGPRSIALPATIIGAGTTIAPSSLVMRGENLPAGTRWMGNPVRPWVEAKKLESSE
                     SSEGSDWESPSPA"
     aSDomain        88..1203
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_epls_AMP-binding.1"
                     /evalue="4.60E-73"
                     /label="epls_AMP-binding.1"
                     /locus_tag="epls"
                     /protein_end="401"
                     /protein_start="29"
                     /score="238.0"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="TTTAAHPKALAIDNGTDRLTYLELAAQIAERVKQLRAAGVGAGDR
                     IGVRVTSGSLELYVSILAVITAGASYVPVDVDDPDERAQLVWGEANVTAVFTDNATLTN
                     FNPPIGGEPRKPGLEDDAWIIFTSGSTGKPKGVAVTHRSAAAFVDAESKIFLPNKPLAP
                     GDRVLAGLSVAFDASCEEMWLAWRYGACLVPAPRSLVKAGADLGAFLTAQSISVVSTVP
                     TLAAMWPAEALQGLRLLILGGEACPPELATRLANTVDSVWNTYGPTEATVVACAAPLVA
                     GQPVAIGLPLAGWKLAVVDAEGNTVRWGEEGELVIGGVGMARYLDPEKDAVKFAPAACF
                     GGERAYRSGDLVRAEKEGLFFVGRNDEQIKLG"
     aSModule        88..3813
                     /complete
                     /domains="nrpspksdomains_epls_AMP-binding.1"
                     /domains="nrpspksdomains_epls_PCP.1"
                     /domains="nrpspksdomains_epls_TIGR02353.1"
                     /locus_tags="epls"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       271..312
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_epls_0001"
                     /evalue="3.20E-03"
                     /label="NRPS-A_a2"
                     /locus_tag="epls"
                     /protein_end="104"
                     /protein_start="90"
                     /score="11.1"
                     /tool="antismash"
                     /translation="LAVITAGASYVPVD"
     CDS_motif       451..510
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_epls_0002"
                     /evalue="1.60E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="epls"
                     /protein_end="170"
                     /protein_start="150"
                     /score="29.8"
                     /tool="antismash"
                     /translation="AWIIFTSGSTGKPKGVAVTH"
     CDS_motif       643..669
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_epls_0003"
                     /evalue="9.90E+01"
                     /label="NRPS-A_a2"
                     /locus_tag="epls"
                     /protein_end="223"
                     /protein_start="214"
                     /score="-3.1"
                     /tool="antismash"
                     /translation="WRYGACLVP"
     CDS_motif       1012..1086
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_epls_0004"
                     /evalue="1.10E-03"
                     /label="NRPS-A_a6"
                     /locus_tag="epls"
                     /protein_end="362"
                     /protein_start="337"
                     /score="11.4"
                     /tool="antismash"
                     /translation="GEEGELVIGGVGMARYLDPEKDAVK"
     CDS_motif       1168..1230
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_epls_0005"
                     /evalue="7.30E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="epls"
                     /protein_end="410"
                     /protein_start="389"
                     /score="27.9"
                     /tool="antismash"
                     /translation="FVGRNDEQIKLGGRRIELGEI"
     aSDomain        1522..1710
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_epls_PCP.1"
                     /evalue="8.80E-14"
                     /label="epls_PCP.1"
                     /locus_tag="epls"
                     /protein_end="570"
                     /protein_start="507"
                     /score="43.4"
                     /tool="antismash"
                     /translation="LAEQWRRVVGVPASPDSDFFDIGGTSLAAAQLVSQLRQRAPTMSV
                     ADIYEYPILSAMAARVDD"
     aSDomain        1774..3813
                     /aSDomain="TIGR02353"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_epls_TIGR02353.1"
                     /evalue="1.10E-161"
                     /label="epls_TIGR02353.1"
                     /locus_tag="epls"
                     /protein_end="1271"
                     /protein_start="591"
                     /score="531.2"
                     /tool="antismash"
                     /translation="QACILICEITFNSGRWLAGVSAMKKFFALRLGPDSWAGVNPLPWW
                     LIAVIWALFMTFPGRLITTALGSRLLTWGIKPGTYRRGGGTHLRLWGAERFAALGNIGA
                     IAGTQWVRRYARLLGCHVGKDVQFHGLPPTTGLASFGDGCSVEPEVDTAGWWLDGDFLH
                     IGFITVGAGAVVGHRSTLMPGTVIEPYATVEGGTVVEGTVAGPDVTPSADEKAYMNERS
                     FFSGLRYTLTLLILDFLPIIPAAPTLALTPILVRDYSDFHNLVMTIIKVSAPGIVLGIF
                     LYVAMSVMFVRLASIFLRPGSYSWHSTAAWAAWITHGTMTQLRATMFPVFASLLTPTWL
                     RLFGARIGKYVEASTIIAIPSLLTVEDGAFLADDVVLSPFELCRGFIRVGPSSVGEKAF
                     VGNSGIVDPGVHVPERSLVGVLGTAPHDMEAGSSWLGRPAISLPRKQDIQVDDSLTFKP
                     PTRLVIFRGLIELCRLTPLLISGMLATGIATGYLWILNTFGYWQAILAGPAMMFSAGFF
                     ALFLTTAVKWILTPRITAGMHRPLWSSFVWRNELADTFIQSLAVPWMIGSFYGTPLLVW
                     WMRTLGAKIGHGVWLDSHHLPEAELCEIGDGASVNRRAVLQTHLFHDRIMRLDKVVLEA
                     GATLGPRSIALPATIIGAGTTIAPSSLVMRGENLPAGTRWMGNPV"
ORIGIN
        1 atgagtcaac ctcactccaa ggatgacctg gctctgtacc cagtcgacga ctgtggtgct
       61 ccccgcactc tcgtggacat tcttgagacg accacagctg cccaccccaa ggcacttgcc
      121 attgacaatg gcacagatcg gttgacgtac ttggagctcg ctgctcaaat cgcggagcgc
      181 gtcaaacagc tcagagctgc cggtgtgggc gctggcgacc gaattggagt tcgagtcacc
      241 tcgggctcac tggagctcta tgtgagcatt ctcgccgtca tcactgccgg tgcctcctac
      301 gtcccagttg atgtggatga ccctgatgag cgtgctcagc tggtctgggg agaagccaat
      361 gtcacggccg tcttcacaga taacgccacc ctgaccaatt tcaaccctcc catcggaggc
      421 gagccaagga aaccgggctt ggaggatgat gcctggatca tcttcacctc cggttccacg
      481 ggcaaaccca agggagtcgc cgtcactcac cgcagcgccg ccgccttcgt cgatgccgaa
      541 tccaaaatct tcttgcccaa caagccgctc gcccccgggg accgtgttct cgccggactc
      601 tccgtcgctt ttgacgcatc gtgtgaggaa atgtggctcg catggagata tggcgcatgc
      661 ctggtccctg ctccccgttc gcttgtcaaa gccggtgccg acctgggcgc cttcctcacc
      721 gcacagtcga tctcggtagt gtcgacggtg cccacactgg ccgcaatgtg gccggcggag
      781 gctctccagg gcctccggct gttgattctc ggcggagagg cctgccctcc cgaattggca
      841 acccggcttg ccaacaccgt cgactccgtg tggaacacat acggccccac cgaggcaacc
      901 gtcgttgctt gcgccgcccc gctcgtcgcc ggacagccag tggccatcgg ccttcctctc
      961 gccggatgga agctcgccgt cgtggatgcc gagggcaaca cggtcagatg gggcgaggag
     1021 ggggagttgg tcattggcgg tgttggcatg gcgcgatacc ttgaccctga gaaggacgcg
     1081 gtcaagttcg cgccagcagc ctgcttcggc ggtgagcgcg cctaccgaag tggcgatctg
     1141 gttcgcgccg agaaggaggg cctcttcttt gtaggtcgca acgatgaaca gatcaaactg
     1201 ggtggtcgcc gcatcgaact cggcgagatc gatgctgcgc tcatgaccct acccggtgtc
     1261 agcgccggtg ccaccgctat ccgccgtagt gagactggaa atcaggtcct cgtcggctac
     1321 gtcgttcgca caggcgctcc gcaggccaat gaccgtggcg ttcttcgccg tctgctgcct
     1381 gccaccctgg tccccatgat cgtcacagtc gacgatattc cagttcgcac ctccggcaaa
     1441 gtcgaccgaa aggccttgcc ctggccacca ccagcctcct cgcacgttga tgcccagccc
     1501 attcacggta ccaccgcttg gctcgccgag caatggcgcc gagtcgttgg cgttcccgct
     1561 tctcctgatt ccgacttctt cgacatcggc ggcaccagct tggctgccgc tcagctcgtg
     1621 tcgcaattga ggcagcgcgc tcccaccatg tccgtcgccg acatctacga gtaccccatc
     1681 ctatcagcca tggccgcgcg cgtggacgac ctggccggca ccaagcagga ggatcgcatg
     1741 gtcaggccaa ccgcacgctg ggtcatgttc gtccaggcct gcattctcat ctgcgagatc
     1801 accttcaact ctgggcgatg gcttgccggc gtctccgcca tgaagaagtt cttcgccctg
     1861 cgcctgggcc cagactcctg ggcaggcgtg aaccctcttc cttggtggct gattgccgtc
     1921 atttgggccc tcttcatgac cttccctggc cgcttgatca ccacagcgct gggctcacgt
     1981 ctactcacgt ggggaatcaa gcctggcacg tacaggcgtg gtggcggtac ccatcttcgt
     2041 ctttggggtg ctgagcggtt tgctgccttg ggaaacatcg gtgccattgc cggaacgcaa
     2101 tgggttcgtc gctacgcacg gctcctgggc tgccatgttg gcaaagacgt ccagttccat
     2161 ggcctccctc ccaccacggg tctcgccagc ttcggcgacg gttgctctgt cgagcctgaa
     2221 gtcgataccg caggttggtg gctcgacggt gatttcttgc acattggatt catcacagtt
     2281 ggtgcgggtg cggtcgtcgg tcatcgatcg actctgatgc ctggcacagt cattgaaccc
     2341 tacgccaccg tcgagggcgg taccgtggtc gagggtaccg tcgctggccc cgatgtcacc
     2401 ccctctgcgg acgagaaggc atacatgaac gagcgaagct tcttctccgg acttcgatac
     2461 accctcacgc ttctcattct cgacttcttg ccaatcattc ctgccgcccc tacattggcc
     2521 ctgacgccca tcttggttcg cgactacagc gacttccaca atctggtcat gaccatcatc
     2581 aaggtttctg ctcctggtat cgttctcggc atcttcctgt acgtcgcgat gagtgtcatg
     2641 ttcgtccgcc tggccagcat cttcctccgt cctggctcct attcatggca cagcaccgcc
     2701 gcctgggcag cgtggatcac gcacggcacc atgacgcaac ttcgcgccac catgttcccc
     2761 gtcttcgcga gcctgctcac gccgacttgg ctgcgcttgt ttggtgctcg catcggcaaa
     2821 tatgtcgagg cttccaccat cattgcgatc ccttcgctcc tgaccgtcga ggacggcgct
     2881 ttccttgccg acgacgtcgt gctgtcgccc tttgagctct gccgcggctt catccgcgtt
     2941 ggcccatcat ctgtcggcga gaaggcgttt gtcggaaact ctggcatcgt cgaccccggc
     3001 gttcacgttc ctgagcgatc tttggtcggt gtgctcggca ccgcccccca cgacatggag
     3061 gctggctcat cgtggctcgg tcgcccggcc atctcgctgc cccgaaagca ggacatccag
     3121 gtcgatgact cgctcacttt caagcctccc acccgactcg tcatcttcag ggggttgatt
     3181 gagctttgca gactgacgcc tctgctgatc agcggcatgt tggccacggg catcgccacg
     3241 ggctacctgt ggattctgaa cacgtttggc tactggcaag ccatcctcgc cggcccggcc
     3301 atgatgttct ccgctggctt cttcgccctg ttcctcacca cggccgtcaa atggattctc
     3361 accccgcgca tcacggccgg aatgcatcgt cccctgtgga gctcattcgt ctggcgcaac
     3421 gagctcgccg acaccttcat ccagtccctc gccgtcccgt ggatgattgg cagcttctac
     3481 ggcacccctc tcctcgtctg gtggatgcgc actctcggcg ccaagatcgg acacggagtc
     3541 tggctggaca gccaccacct tcccgaggct gagctctgtg agattggaga cggcgccagc
     3601 gtgaaccgcc gtgccgttct gcagacccac ctgttccacg acagaatcat gcgcctcgac
     3661 aaggtcgtcc tcgaggccgg cgccaccctc ggcccgcgct ccatcgccct cccagccacc
     3721 atcatcggag caggcaccac cattgccccg tcctcgctgg tgatgcgcgg cgagaacctg
     3781 cccgccggca cccgctggat gggcaacccc gtcagaccgt gggtcgaggc caagaagctg
     3841 gagagctcag agagctcaga gggttcagac tgggagtctc cctctccagc gtaa
//
