LOCUS       BGC0002175              7155 bp    DNA     linear   CON 22-DEC-2016
DEFINITION  Aspergillus oryzae RIB40 DNA, chromosome 4.
ACCESSION   BGC0002175
VERSION     BGC0002175
KEYWORDS    .
SOURCE      Aspergillus oryzae RIB40
  ORGANISM  Aspergillus oryzae RIB40
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus; Aspergillus subgen. Circumdati.
COMMENT     This genome assembly has been updated so
            as to reflect the location and orientation of
            contigs on the chromosomes. Gap lengths were
            estimated using the results from Optical
            Mapping (http://www.bio.nite.go.jp/dogan/project/view/AO). This
            Aspergillus oryzae RIB40 genome consists of a nuclear genome
            assembly represented by eight chromosomes (assembled from 24
            entries) and three unplaced contigs (AP007156, AP007160 and
            AP007177) and a complete mitochondrial genome (AP007176).
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:05:38
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7155
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7155
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[214:6865]"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      215..6865
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     cand_cluster    1..7155
                     /SMILES="CC(=O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..7155
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002175"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7155
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /subregion_numbers="1"
                     /tool="antismash"
     exon            215..501
                     /locus_tag="AO090102000545"
                     /number=5
                     /old_locus_tag="AO070310000109"
     gene            order(215..501,554..845,904..4335,4400..5155,5207..6865)
                     /locus_tag="AO090102000545"
                     /old_locus_tag="AO070310000109"
     CDS             join(215..501,554..845,904..4335,4400..5155,5207..6865)
                     /NRPS_PKS="Domain: SAT (7-244). E-value: 3.3e-90. Score:
                     293.7. Matches aSDomain:
                     nrpspksdomains_AO090102000545_SAT.1"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (378-809). E-value:
                     3.4e-149. Score: 489.2. Matches aSDomain:
                     nrpspksdomains_AO090102000545_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (913-1212). E-value: 2.5e-85.
                     Score: 278.3. Matches aSDomain:
                     nrpspksdomains_AO090102000545_PKS_AT.1"
                     /NRPS_PKS="Domain: PT (1290-1604). E-value: 6.2e-125.
                     Score: 408.6. Matches aSDomain:
                     nrpspksdomains_AO090102000545_PT.1"
                     /NRPS_PKS="Domain: ACP (1654-1718). E-value: 1.5e-17.
                     Score: 55.6. Matches aSDomain:
                     nrpspksdomains_AO090102000545_ACP.1"
                     /NRPS_PKS="Domain: PP-binding (1771-1833). E-value: 3e-16.
                     Score: 51.6. Matches aSDomain:
                     nrpspksdomains_AO090102000545_PP-binding.1"
                     /NRPS_PKS="Domain: Thioesterase (1887-2135). E-value:
                     2.5e-30. Score: 98.4. Matches aSDomain:
                     nrpspksdomains_AO090102000545_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     itr_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 194.6; E-value:
                     4.8e-59)"
                     /gene_kind="biosynthetic"
                     /locus_tag="AO090102000545"
                     /note="unnamed protein product; polyketide synthase modules
                     and related proteins"
                     /old_locus_tag="AO070310000109"
                     /protein_id="BAE61567.1"
                     /sec_met_domain="itr_KS (E-value: 1.6e-165, bitscore:
                     537.1, seeds: 96, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 4.5e-87, bitscore: 278.3,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.4e-18, bitscore:
                     51.6, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEGPRGVYLFGDQTSDFDAGLRRLLQVKNNTIVASFFQRCFHALR
                     QEIARLSPSERKIFPRFTSIVDLLARHRESDPNPALESALTCIYQLGCFINYYGDLGNV
                     YPSASDCHIVGLCAGLLSSAAVSCSNNVGELLPAAVEAVVVALRLGLCVLKVRELVSSD
                     QASSTSWSVLISGISEKDASQLIGEFTAERAIPPSSKPYISAVGYNSITISAPPKVLDD
                     LIDSRLSKSHKPVRAQIHGPYHAAHLYYGRDVDRIIESCHNEVVSNYTPRIPVLSSTTG
                     QPIEAKHMKDLLKAALEEILLRQLCWEKVTDACYSILKTARHQPCKLFPISSTATQSLF
                     TALTKAGITDIEVENGLGDVPTNPKDNLNISGRADCSKIAIIGMSGRFPEADGTESFWD
                     LLYNGLDVHRKVPAERWDVDAHVDPTGTKRNTSKVPYGCWINEPGLFDPRFFNMSPREA
                     LQADPAQRLALLTAYEALEMAGFIPDSTPSTQRDRVGLFYGMTSDDYREINSGQDIDTY
                     FIPGGNRAFTPGRINYYFKFSGPSVSVDTACSSSLAAIHMACNSIWRNDCDAAIAGGVN
                     ILTNPDNHAGLDRGHFLSRTGNCNTFDDGADGYCRADGVGTIILKRLEDAQADNDPILG
                     VINGAYTNHSAEAVSITRPHVGAQAFIFNKLLNDANIDPKDVSYVEMHGTGTQAGDAVE
                     MQSVLDTFAPDYRRGPGQSLHLGSAKANVGHGESASGVTALVKVLLMMKKNTIPPHCGI
                     KTKINHNFPTDLAQRNVHIAFQPTPWNRPASGKRQCFINNFSAAGGNTALLMEDAPIAE
                     VKGQDTRPVHVVSVSARSQSALKNNINSLVKYIDEQGRSFNVNEADFIPSLAYTTTARR
                     IHHPFRVTAIGSSLQELRDSLNNSSRLESFTPVPATAPGVGFVFAGQGAQHTGMGRQLY
                     EKCSQFRATMQHFDCISQNQGFPSILPLVDGSVPVEELGPIVTQLGTTCLQMALVNYWG
                     SLGIKPAFVLGHSLGEFAALNTAGVLSTSDTIYLCGRRATLLTEYCQVGTHAMLAVKAS
                     YPQVKQLLKEGVDEVACVNSPSETVVSGLTADIDDLAQRCSTEGWKSTKLRVPFAFHSA
                     QVTPILERFQEEAQGVTFRKPSLPFVSSLLGEVITESNYDVLGAQYMVKQCRKSVNFLG
                     ALEATRYAKLMTDKTVWLEVGAHTICSGMIKATFGPQVTTVASLRREENAWKVLSNSLS
                     ALHLAGIDINWKEYHQDFSSSHQVLPLPSYKWDLKNYWIPYTNNFCLTKGAPQTAIQAA
                     PQTTFLTTAAQKVVESRDDGTTATVVVQNDIADPELNRVIQGHKVNGAALCPSSLYADI
                     AQTLGEYLIEKYKPEFKDLGLDVCDMVVPKPLIAKGGEQLFRVSAIANWAEKKASVQVY
                     AVNADGKKTVDHAYCTVKFFDTNASELEWKRISYLVKRSIDSLHQNAETGEAHRIQRGM
                     VYKLFSALVDYDENFKSIREVILDSDNNEATARVKFQAPPGNFHRNPFWIDSFGHLSGF
                     IMNASDATDSKNQVFVNHGWDSMRCLKKFSPDVTYRTYVRMQPWQNNIWAGDVYIFEGD
                     DIIAVFGGVKFQALARKILDTVLPPVGGSKAPITAKSPPPARTQKANTGAKTRPKAPVP
                     SKSFTKSSGPSVVVRALSILASEVGLAESEISDDMVFADYGVDSLLSLTVTGRYREELN
                     LDLDSSVFTDHPTVNDFKRLIAQVSPSESHDGSSSEQESNFSFNGGESSSASTPDITSP
                     PNEKVAQVEQNGTMKEIRNIMAEEIGVPAEEIDPDENLGEMGMDSLLSLTVLGRIRETL
                     DMDLPGEFFIENQTLNDIEVALDLKPKTTSAPIPMPEPVKFPEAIHDLQPKLAQHPKAT
                     SILLQGNPRTATKTLFLFPDGSGSATSYATIPGLSPDVCVYGLNCPYMKTPEKLKCSLD
                     ELTAPYVAEIRRRQPKGPYSFGGWSAGGICAYDAARHLMFEEGEQVDRLLLLDTPFPIG
                     LEKLPQRLYGFFNSIGLFGEGKTAPPSWLLPHFLAFIDALDAYKAAPLPFKDEKWAKKL
                     PKTYIIWAKDGVCGKPGDPRPDPPTDGSKDPKEMVWLLNDRTDLGPNKWDTLVGPENIG
                     GITVMEDANHFTMTKGEKAKELSTFMANAMA"
     aSDomain        join(236..501,554..845,904..1056)
                     /aSDomain="SAT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AO090102000545_SAT.1"
                     /evalue="3.30E-90"
                     /label="AO090102000545_SAT.1"
                     /locus_tag="AO090102000545"
                     /protein_end="244"
                     /protein_start="7"
                     /score="293.7"
                     /tool="antismash"
                     /translation="YLFGDQTSDFDAGLRRLLQVKNNTIVASFFQRCFHALRQEIARLS
                     PSERKIFPRFTSIVDLLARHRESDPNPALESALTCIYQLGCFINYYGDLGNVYPSASDC
                     HIVGLCAGLLSSAAVSCSNNVGELLPAAVEAVVVALRLGLCVLKVRELVSSDQASSTSW
                     SVLISGISEKDASQLIGEFTAERAIPPSSKPYISAVGYNSITISAPPKVLDDLIDSRLS
                     KSHKPVRAQIHGPYH"
     exon            554..845
                     /locus_tag="AO090102000545"
                     /number=4
                     /old_locus_tag="AO070310000109"
     exon            904..4335
                     /locus_tag="AO090102000545"
                     /number=3
                     /old_locus_tag="AO070310000109"
     aSDomain        1459..2751
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AO090102000545_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="3.40E-149"
                     /label="AO090102000545_PKS_KS.1"
                     /locus_tag="AO090102000545"
                     /protein_end="809"
                     /protein_start="378"
                     /score="489.2"
                     /tool="antismash"
                     /translation="IAIIGMSGRFPEADGTESFWDLLYNGLDVHRKVPAERWDVDAHVD
                     PTGTKRNTSKVPYGCWINEPGLFDPRFFNMSPREALQADPAQRLALLTAYEALEMAGFI
                     PDSTPSTQRDRVGLFYGMTSDDYREINSGQDIDTYFIPGGNRAFTPGRINYYFKFSGPS
                     VSVDTACSSSLAAIHMACNSIWRNDCDAAIAGGVNILTNPDNHAGLDRGHFLSRTGNCN
                     TFDDGADGYCRADGVGTIILKRLEDAQADNDPILGVINGAYTNHSAEAVSITRPHVGAQ
                     AFIFNKLLNDANIDPKDVSYVEMHGTGTQAGDAVEMQSVLDTFAPDYRRGPGQSLHLGS
                     AKANVGHGESASGVTALVKVLLMMKKNTIPPHCGIKTKINHNFPTDLAQRNVHIAFQPT
                     PWNRPASGKRQCFINNFSAAGGNTALLMEDAP"
     aSModule        1459..5593
                     /complete
                     /domains="nrpspksdomains_AO090102000545_PKS_KS.1"
                     /domains="nrpspksdomains_AO090102000545_PKS_AT.1"
                     /domains="nrpspksdomains_AO090102000545_PT.1"
                     /domains="nrpspksdomains_AO090102000545_ACP.1"
                     /iterative
                     /locus_tags="AO090102000545"
                     /monomer_pairings="mal -> mal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       1939..1986
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AO090102000545_0001"
                     /evalue="4.20E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="AO090102000545"
                     /protein_end="554"
                     /protein_start="538"
                     /score="18.8"
                     /tool="antismash"
                     /translation="GPSVSVDTACSSSLAA"
     CDS_motif       2128..2166
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AO090102000545_0002"
                     /evalue="3.60E-03"
                     /label="PKSI-KS_m4"
                     /locus_tag="AO090102000545"
                     /protein_end="614"
                     /protein_start="601"
                     /score="10.6"
                     /tool="antismash"
                     /translation="FDDGADGYCRADG"
     CDS_motif       2359..2406
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AO090102000545_0003"
                     /evalue="5.20E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="AO090102000545"
                     /protein_end="694"
                     /protein_start="678"
                     /score="12.3"
                     /tool="antismash"
                     /translation="YVEMHGTGTQAGDAVE"
     CDS_motif       3061..3114
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AO090102000545_0004"
                     /evalue="8.70E-06"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="AO090102000545"
                     /protein_end="930"
                     /protein_start="912"
                     /score="18.1"
                     /tool="antismash"
                     /translation="FVFAGQGAQHTGMGRQLY"
     aSDomain        3064..3960
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AO090102000545_PKS_AT.1"
                     /evalue="2.50E-85"
                     /label="AO090102000545_PKS_AT.1"
                     /locus_tag="AO090102000545"
                     /protein_end="1212"
                     /protein_start="913"
                     /score="278.3"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="VFAGQGAQHTGMGRQLYEKCSQFRATMQHFDCISQNQGFPSILPL
                     VDGSVPVEELGPIVTQLGTTCLQMALVNYWGSLGIKPAFVLGHSLGEFAALNTAGVLST
                     SDTIYLCGRRATLLTEYCQVGTHAMLAVKASYPQVKQLLKEGVDEVACVNSPSETVVSG
                     LTADIDDLAQRCSTEGWKSTKLRVPFAFHSAQVTPILERFQEEAQGVTFRKPSLPFVSS
                     LLGEVITESNYDVLGAQYMVKQCRKSVNFLGALEATRYAKLMTDKTVWLEVGAHTICSG
                     MIKATFGPQVTTVASLRR"
     CDS_motif       3298..3414
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AO090102000545_0005"
                     /evalue="1.80E-12"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="AO090102000545"
                     /protein_end="1030"
                     /protein_start="991"
                     /score="39.2"
                     /tool="antismash"
                     /translation="GIKPAFVLGHSLGEFAALNTAGVLSTSDTIYLCGRRATL"
     aSDomain        join(4195..4335,4400..5155,5207..5251)
                     /aSDomain="PT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AO090102000545_PT.1"
                     /evalue="6.20E-125"
                     /label="AO090102000545_PT.1"
                     /locus_tag="AO090102000545"
                     /protein_end="1604"
                     /protein_start="1290"
                     /score="408.6"
                     /tool="antismash"
                     /translation="TTAAQKVVESRDDGTTATVVVQNDIADPELNRVIQGHKVNGAALC
                     PSSLYADIAQTLGEYLIEKYKPEFKDLGLDVCDMVVPKPLIAKGGEQLFRVSAIANWAE
                     KKASVQVYAVNADGKKTVDHAYCTVKFFDTNASELEWKRISYLVKRSIDSLHQNAETGE
                     AHRIQRGMVYKLFSALVDYDENFKSIREVILDSDNNEATARVKFQAPPGNFHRNPFWID
                     SFGHLSGFIMNASDATDSKNQVFVNHGWDSMRCLKKFSPDVTYRTYVRMQPWQNNIWAG
                     DVYIFEGDDIIAVFGGVKFQALARKILDTVLPP"
     CDS_motif       join(4321..4335,4400..4411)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AO090102000545_0006"
                     /evalue="4.50E+01"
                     /label="PKSI-KS_m3"
                     /locus_tag="AO090102000545"
                     /protein_end="1341"
                     /protein_start="1332"
                     /score="-2.0"
                     /tool="antismash"
                     /translation="ALCPSSLYA"
     exon            4400..5155
                     /locus_tag="AO090102000545"
                     /number=2
                     /old_locus_tag="AO070310000109"
     exon            5207..6865
                     /locus_tag="AO090102000545"
                     /number=1
                     /old_locus_tag="AO070310000109"
     aSDomain        5402..5593
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AO090102000545_ACP.1"
                     /evalue="1.50E-17"
                     /label="AO090102000545_ACP.1"
                     /locus_tag="AO090102000545"
                     /protein_end="1718"
                     /protein_start="1654"
                     /score="55.6"
                     /tool="antismash"
                     /translation="LSILASEVGLAESEISDDMVFADYGVDSLLSLTVTGRYREELNLD
                     LDSSVFTDHPTVNDFKRLI"
     aSDomain        5753..5938
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AO090102000545_PP-binding.1"
                     /evalue="3.00E-16"
                     /label="AO090102000545_PP-binding.1"
                     /locus_tag="AO090102000545"
                     /protein_end="1833"
                     /protein_start="1771"
                     /score="51.6"
                     /tool="antismash"
                     /translation="EIRNIMAEEIGVPAEEIDPDENLGEMGMDSLLSLTVLGRIRETLD
                     MDLPGEFFIENQTLNDI"
     aSModule        5753..6844
                     /domains="nrpspksdomains_AO090102000545_PP-binding.1"
                     /domains="nrpspksdomains_AO090102000545_Thioesterase.1"
                     /final_module
                     /incomplete
                     /locus_tags="AO090102000545"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        6101..6844
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AO090102000545_Thioesterase.1"
                     /evalue="2.50E-30"
                     /label="AO090102000545_Thioesterase.1"
                     /locus_tag="AO090102000545"
                     /protein_end="2135"
                     /protein_start="1887"
                     /score="98.4"
                     /tool="antismash"
                     /translation="TLFLFPDGSGSATSYATIPGLSPDVCVYGLNCPYMKTPEKLKCSL
                     DELTAPYVAEIRRRQPKGPYSFGGWSAGGICAYDAARHLMFEEGEQVDRLLLLDTPFPI
                     GLEKLPQRLYGFFNSIGLFGEGKTAPPSWLLPHFLAFIDALDAYKAAPLPFKDEKWAKK
                     LPKTYIIWAKDGVCGKPGDPRPDPPTDGSKDPKEMVWLLNDRTDLGPNKWDTLVGPENI
                     GGITVMEDANHFTMTKGEKAKELSTF"
     CDS_motif       6278..6352
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AO090102000545_0007"
                     /evalue="1.20E-11"
                     /label="NRPS-te1"
                     /locus_tag="AO090102000545"
                     /protein_end="1971"
                     /protein_start="1946"
                     /score="36.9"
                     /tool="antismash"
                     /translation="QPKGPYSFGGWSAGGICAYDAARHL"
ORIGIN
        1 ttggctggaa acgagcttgt gattgccctt tgagtgatcg gtggaatgct cgtccaagtt
       61 ctttgcatgc cgtaattgag acatagtctc cactgtcgca tgtggtagta actctatctt
      121 ggtataaatc atacttaata tctagccaat cgctttggga aaacaatttg atcagcatct
      181 ctgcgactct tgactggcac tttcttgacc caacatggag gggccacgcg gcgtctatct
      241 cttcggagac cagacaagtg atttcgacgc cggcttacgt cgcctcctac aagtaaagaa
      301 taacacaatt gttgcatcgt tcttccagag atgctttcac gctttgcgcc aagagatcgc
      361 gaggctttca ccatctgaac ggaagatctt cccccggttt acgagcatag tggatctact
      421 ggcgcgtcac cgggagtcag accctaatcc ggctctggag agtgcgttga cctgtatcta
      481 tcaattggga tgctttataa agtacgtgta actgcagatc ctgacccgtt tgaacgagcc
      541 taacctgaga tagctactac ggagaccttg gaaacgtgta cccatctgct tcagactgcc
      601 atatagttgg cctgtgcgcg ggtcttctta gttctgcagc tgtaagctgt tcgaacaatg
      661 ttggagaatt gctccccgct gcggttgaag cggtggtggt agctctccga cttggtctat
      721 gcgtccttaa agttcgagag ctggtgagct ctgaccaagc gtcgtcaaca agctggtcag
      781 tcttgatttc agggattagc gagaaagatg cctcgcagct tataggagaa ttcactgctg
      841 aacgggtaag tcaattgatc tgaaatagtt tgcaggacag aatgttctaa ccactggata
      901 aaggcaattc ctccttcatc caaaccgtat atcagtgcgg tgggatataa cagtataacc
      961 atcagcgcac cgcctaaggt ccttgatgat ttaattgatt ctaggctgtc taagagccat
     1021 aagccggtga gggcgcaaat ccatggtcct taccatgcag cacatctgta ctatggccga
     1081 gatgtcgaca ggatcatcga aagctgccat aatgaggtcg tttcaaacta cacaccccgt
     1141 atccccgtac tatcaagtac tacgggacag ccgatagagg ccaaacacat gaaagatcta
     1201 cttaaggccg cccttgaaga gattctacta cgtcaactat gctgggagaa agtgaccgat
     1261 gcctgctatt ccatattaaa aactgctcgt catcaaccat gcaagttgtt cccaatttca
     1321 agcactgcga cacaaagctt gtttacagct cttacgaaag ccgggataac cgacatcgaa
     1381 gtggaaaatg ggctcggaga tgttcccacg aacccgaagg acaaccttaa catcagcggc
     1441 agggcggact gctccaagat agctatcatt ggcatgtctg gacgattccc agaagctgat
     1501 ggcacagaga gtttctggga ccttctgtat aatggcctcg atgtacaccg gaaggtgcct
     1561 gcagagcgtt gggatgttga tgcccacgtt gatcctaccg gaacaaaacg gaacaccagc
     1621 aaggttccat acggatgctg gataaacgaa ccggggttat ttgacccccg cttcttcaat
     1681 atgtcgccac gcgaagccct ccaggcagat cccgctcaaa gacttgcatt gctcacggcc
     1741 tatgaagctc ttgaaatggc cggctttatc cccgacagca ccccttctac acagagggat
     1801 cgagtcggcc tcttctatgg aatgactagc gatgactatc gggagataaa tagtggtcaa
     1861 gatattgata cttactttat ccctggtggg aatcgtgctt tcacacctgg ccggataaac
     1921 tactatttca agttcagtgg gcccagcgtc agcgttgata cagcttgttc ttcaagtctt
     1981 gcggctattc atatggcttg caattcgatc tggagaaatg attgcgatgc tgctattgct
     2041 ggaggtgtca atatattgac aaaccctgat aaccatgccg gtcttgaccg tggccatttc
     2101 ctgtccagaa ccgggaattg caacacattt gacgatggtg ctgatggcta ctgtagagca
     2161 gatggagtgg gtacaatcat tctcaagcgg ctggaagacg ctcaggcgga caacgatcca
     2221 atcctcggtg tgatcaatgg agcctatacc aatcattcgg cagaagcagt ctcgattacc
     2281 cgccctcatg ttggcgcaca agcgtttatc tttaataagc tattgaacga tgccaatatc
     2341 gaccctaagg acgtcagcta cgttgaaatg catggaactg gtactcaagc tggggatgcg
     2401 gtggaaatgc aatcggtctt ggatacgttt gctcccgact accgccgtgg accaggacag
     2461 tctctccatc ttggttccgc caaagcaaat gttgggcatg gagagtcagc atctggtgta
     2521 actgcacttg tgaaagtgct gctaatgatg aagaagaata ccataccccc tcattgtggt
     2581 ataaagacta agatcaacca caacttcccc acggatctcg cgcaacgaaa tgtccacatt
     2641 gcctttcaac ctaccccttg gaacagaccg gcttccggaa agcggcagtg cttcattaac
     2701 aacttttcgg cggctggtgg aaataccgct cttttgatgg aagacgctcc aatcgctgag
     2761 gttaaggggc aggacactcg acctgttcac gttgtgtctg tatcggcacg atcccagagt
     2821 gcgctcaaaa acaacatcaa ctctctcgta aaatacatcg acgaacaagg aaggtcattc
     2881 aatgtgaacg aggcagactt tatcccaagc ttggcataca ccaccacagc acggcgtatc
     2941 catcacccat tccgtgtcac agctatcggg tctagtttgc aggagctgcg tgactcactt
     3001 aacaacagct ctcgtctgga aagctttacc cctgtccctg cgacggcccc tggcgtaggg
     3061 ttcgtgttcg ctggccaagg agctcagcac accggaatgg gaaggcaact atacgaaaaa
     3121 tgctctcaat tccgggcaac aatgcagcac ttcgattgca ttagtcaaaa ccaagggttt
     3181 ccttcgatcc ttcccttggt tgacggaagc gtgcccgtgg aggagctggg ccctatcgtg
     3241 acacagctcg gcaccacatg tcttcagatg gctttggtca actattgggg ttcactaggt
     3301 ataaaacctg cgttcgttct tgggcatagt ctcggggagt ttgctgcttt gaataccgca
     3361 ggagtattat cgacttccga taccatctac ctttgtggcc gtcgggctac cctccttaca
     3421 gaatactgcc aggttgggac acacgccatg ctggctgtca aggcttccta cccccaggtc
     3481 aagcagttac tgaaagaagg tgtggatgaa gttgcctgtg tcaactcacc cagtgagaca
     3541 gtcgtcagtg gcctcaccgc tgatattgat gacttggctc aaaggtgttc cactgaaggt
     3601 tggaagtcca ctaaactaag ggtaccgttc gctttccatt ctgcccaagt tactccaatt
     3661 cttgaacggt ttcaagaaga ggcccagggt gtcacgttcc gtaagccgtc gttaccgttt
     3721 gtttcctcac tccttgggga agtcatcacc gaatctaatt acgatgtcct gggagctcaa
     3781 tatatggtga agcagtgccg gaagtcggtg aacttccttg gtgctcttga ggccaccaga
     3841 tatgcgaaat tgatgactga taagactgtc tggctggaag ttggtgccca taccatttgc
     3901 tctggtatga tcaaagcaac attcggtccc caggttacca ctgtggcatc tcttcgccga
     3961 gaggagaatg catggaaggt cctctccaat agtctatcgg cccttcattt ggctggcatt
     4021 gatattaatt ggaaagaata tcatcaagac ttcagctcca gccaccaggt gctcccactt
     4081 ccttcttaca agtgggatct caagaactac tggataccct acactaacaa tttctgcctt
     4141 acgaagggtg ctccccaaac tgcaattcaa gctgcaccac aaactacatt cctgaccact
     4201 gctgcgcaaa aggttgttga gagtcgcgac gacggtacaa cagcgactgt cgtggtgcaa
     4261 aatgacatcg ctgatcctga gttgaaccgt gttatccaag gtcacaaggt caatggagcc
     4321 gcactttgcc catcggtaag tattgcatgc attgccagac tatcttgtgt tataattcgg
     4381 ctacttacgt attgcctagt cactctacgc agatattgcc cagacacttg gagagtatct
     4441 tattgagaaa tacaaacccg agttcaaaga tcttggtctc gatgtgtgtg acatggtcgt
     4501 accgaagcca ctcatcgcga agggaggaga gcagctcttt agagtctctg ctattgctaa
     4561 ttgggctgag aagaaggctt cagttcaagt atacgccgtt aatgctgacg gcaaaaagac
     4621 cgtggatcat gcgtattgta cggtgaagtt ctttgatacc aatgcctccg agctcgagtg
     4681 gaagagaatc tcgtacctgg tcaagagaag catcgacagt cttcaccaga atgcggagac
     4741 aggggaggct caccgtatcc agcgaggaat ggtctataaa cttttcagcg cgttggtcga
     4801 ttatgatgaa aatttcaagt cgattcgcga ggttatcctg gacagcgaca ataatgaggc
     4861 caccgctcgt gtcaaattcc aagcaccgcc aggaaatttc caccgaaacc cattctggat
     4921 tgacagtttc ggtcacttgt ccggattcat tatgaatgcg agcgacgcga ccgactctaa
     4981 gaaccaagta tttgttaacc atggatggga ttcgatgcgt tgcctgaaga agttctcgcc
     5041 tgatgtcact tatcgcactt atgtgaggat gcagccatgg caaaacaaca tttgggctgg
     5101 agatgtttat atctttgagg gcgacgatat tattgctgtc ttcggaggtg tgaaggtggg
     5161 tacctcacta ctgattttgg ttcctgctta ctgacatgat aattagttcc aagcactggc
     5221 acgcaagata cttgacactg ttcttccccc tgttggcggt tcaaaggcac caattacagc
     5281 gaaatcacca cctccagctc gcactcagaa ggccaacacc ggcgccaaga cccgtcctaa
     5341 agcacctgtt ccttccaagt cgttcaccaa atcttctggg ccgagtgttg tcgtacgcgc
     5401 actcagcatt ctggcctcag aagttggcct ggcagagtct gaaatctcag acgacatggt
     5461 gtttgcggac tacggtgtag actcactcct ctcccttaca gttactggca ggtatcgtga
     5521 agagttgaac ctcgatttgg actcctctgt gtttaccgat catccaactg tcaacgactt
     5581 caagcggctc atcgcccaag tgagtccttc agagagccat gatggttcct ccagtgaaca
     5641 agagtcgaat ttctctttca acggtggcga gtcctcaagc gcaagcacac ctgacataac
     5701 gtcaccgccg aatgagaagg tagctcaagt cgagcaaaac ggcaccatga aggaaatccg
     5761 taacatcatg gcggaggaga tcggtgtacc cgcagaagag atcgaccctg acgagaactt
     5821 gggagagatg ggtatggact cgcttctctc ccttactgtt cttggaagaa tacgggagac
     5881 tttggacatg gacctgccag gagagttctt catcgaaaac cagaccctca atgatataga
     5941 ggtggctttg gacctaaaac ccaagactac ctctgctcca attcctatgc cagagccagt
     6001 gaaattccct gaagctatcc acgacctcca gccaaagctt gctcaacatc ccaaggccac
     6061 atccatcctg ttacaaggaa accccaggac agcaacaaag acgttattct tgtttcctga
     6121 cggctctggc tcagctacat cttacgctac catccccgga ctctctcctg acgtctgcgt
     6181 ttacgggttg aattgcccat atatgaagac acctgagaag ctcaaatgca gcctagatga
     6241 actcactgcg ccctatgtag cagagattcg tcgtcggcaa cccaagggtc cttacagctt
     6301 cggtggctgg tcagcaggag ggatctgtgc atatgatgcg gcacgccatc taatgtttga
     6361 ggaaggtgaa caagtcgacc gcttgcttct ccttgatacc cccttcccca tcggcctcga
     6421 gaagctgccg cagagattgt acggcttctt caactctatc ggtctcttcg gtgaaggtaa
     6481 aacggcacca ccctcctggc tcctacccca cttcctagcc tttatcgacg ctctcgacgc
     6541 atacaaggcc gcgccccttc cattcaaaga cgagaaatgg gccaagaaac tgcccaagac
     6601 ttatatcatc tgggccaagg acggtgtttg cggtaagccg ggagatcccc ggcctgatcc
     6661 cccgacagac ggttccaagg atcccaagga gatggtctgg cttcttaatg accggaccga
     6721 tctgggacct aacaagtggg atacattggt tggacctgag aatattggtg gaatcacagt
     6781 aatggaagat gctaatcatt ttacgatgac gaagggcgaa aaagcgaaag agttgtctac
     6841 atttatggct aacgccatgg cttaaagact gatgaagctt attatgttat tttctatcat
     6901 tatattgcac ttattttccc tatcgttcat atggcttgtt catgtcgttg atctcatgtt
     6961 ttgaataacc agggattaca gcactatcat tcagtaagta acggcttgca ctaattgtac
     7021 gtaattaatc aattatacat ttgaacaagg agatattgtc acgtagcata atcaatacga
     7081 aaacagccta catattgaaa catcgctaat attggatgtg gaaaacgaag tcctcgggca
     7141 gtagaacagt agaaa
//
