BGC0002186 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002186 (Fusarium graminearum PH-1 (Gibberella zeae PH-1))

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	62,600	High	fusaristatin A	NRPS:Type I+PKS

BGC0002186 - Region 1 - NRPS,T1PKS

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Location: 1 - 62,600 nt. (total: 62,600 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 62,601
Label		BGC0002186

Tailoring functions

Oxidoreductases: oxygenase

FGSG_08207: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Transferases: aminotransferase

FGSG_08205: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Ligases: ligase synthase

FGSG_08208: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

FGSG_08209: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002186.2	1.94	NRPS, PKS	fusaristatin A	Fusarium graminearum PH-1
BGC0002191.2	0.90	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002135.2	0.81	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001166.5	0.76	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.76	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001240.4	0.69	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002186.2	0.99	NRPS, PKS	fusaristatin A	Fusarium graminearum PH-1
BGC0002191.2	0.75	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002135.2	0.72	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001240.4	0.68	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000342.4	0.67	NRPS	enniatin	Fusarium equiseti
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 62600:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser - X - Gln) + (Me-pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 53626:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser - X - Gln) + (Me-pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 14828 - 62600:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser - X - Gln) + (Me-pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FGSG_08209: Ser - X - Gln

Search NORINE for peptide: strict or relaxed

AMP-binding (1006..1404): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVSSVFAIFK
Nearest Stachelhaus code(s):: Ser DVSSVFAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2101..2500): X

nrpys: Ibu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQVQAIVYK
Nearest Stachelhaus code(s):: Ibu DVQVQAIVYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3204..3609): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVALLGSITK
Nearest Stachelhaus code(s):: Gln DVALLGSITK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

FGSG_08208: pk

PKS_AT (594..912): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 54.2%; Ethylmalonyl-CoA: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 59.8
Methylmalonyl-CoA: 58.8
Malonyl-CoA: 41.9
trans-1,2-CPDA: 29.7
Propionyl-CoA: 27.6
Ethylmalonyl-CoA: 20.1
fatty_acid: 19.3
Isobutyryl-CoA: 16.0
CHC-CoA: 16.0
inactive: 15.2
2-Methylbutyryl-CoA: 15.1
Benzoyl-CoA: 12.2
Acetyl-CoA: 10.9
3-Methylbutyryl-CoA: 8.3

Link to PARAS substrate predictor