BGC0002188 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002188 (Fusarium pseudograminearum CS3096)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	85,477	High	W493 B/W493 A	NRPS:Type I+PKS

BGC0002188 - Region 1 - NRPS,T1PKS

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Location: 1 - 85,477 nt. (total: 85,477 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 85,478
Label		BGC0002188

Tailoring functions

Ligases: ligase synthase

FPSE_09183: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FPSE_09184: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FPSE_09187: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002188.3	1.45	NRPS, PKS	W493 B, W493 A	Fusarium pseudograminearum CS3096
BGC0002191.2	0.86	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.62	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.62	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.62	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000107.3	0.61	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0002175.3	0.61	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001257.3	0.61	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.61	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002188.3	0.98	NRPS, PKS	W493 B, W493 A	Fusarium pseudograminearum CS3096
BGC0002191.2	0.75	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001240.4	0.70	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000357.5	0.69	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 85477:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - D-Ala - Ala - Gln - D-Tyr - X) + (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 66918:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - D-Ala - Ala - Gln - D-Tyr - X) + (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 37557 - 85477:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - D-Ala - Ala - Gln - D-Tyr - X) + (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FPSE_09183: X - Ala - Ala - Gln - Tyr - X

Search NORINE for peptide: strict or relaxed

AMP-binding (988..1386): X

nrpys: Thr, Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQTIMAIHK
Nearest Stachelhaus code(s):: Thr or Ser DAQTIMAIHK (100% 8Å match); Thr DAQTIMAIHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2542..2934): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVATVTAIWK
Nearest Stachelhaus code(s):: Ala DVATVTAIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4097..4488): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVATVTAIWK
Nearest Stachelhaus code(s):: Ala DVATVTAIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5203..5605): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVQIIGGIHK
Nearest Stachelhaus code(s):: Gln DVQIIGGIHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6305..6700): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQTIVGIAK
Nearest Stachelhaus code(s):: Tyr DAQTIVGIAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (7854..8254): X

nrpys: Ile, Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAYFIGAIFK
Nearest Stachelhaus code(s):: Ile or Val DAYFIGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

FPSE_09184: X

AMP-binding (44..471): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GLTYVGGGIK
Nearest Stachelhaus code(s):: Ala DLGIVGGMIK (29% 8Å match); nohBza GLGLVGAIIK (26% 8Å match)
Stachelhaus code match:: 60% (weak)

FPSE_09187: mal

PKS_AT (580..903): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 58.3%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 75.7
Methylmalonyl-CoA: 67.2
Methoxymalonyl-CoA: 66.9
Isobutyryl-CoA: 38.1
Ethylmalonyl-CoA: 38.0
fatty_acid: 26.8
Propionyl-CoA: 22.3
inactive: 21.7
trans-1,2-CPDA: 18.6
Benzoyl-CoA: 17.0
3-Methylbutyryl-CoA: 13.9
CHC-CoA: 13.3
2-Methylbutyryl-CoA: 7.4
Acetyl-CoA: 0.0

Link to PARAS substrate predictor