BGC0002232 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002232 (Claviceps purpurea 20.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,indole	1	55,951	ergotamine/ergocryptine	terpene	76%

BGC0002232 - Region 1 - NRPS,indole

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Location: 1 - 55,951 nt. (total: 55,951 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
indole: (indsynth or dmat or indole_PTase)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 55,952
Label		BGC0002232

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001241	1.23	Terpene	ergotamine	Claviceps fusiformis
BGC0002232	1.23	Alkaloid	ergotamine	Claviceps purpurea 20.1
BGC0001267	0.59	Terpene	lysergic acid, elymoclavine	Claviceps fusiformis
BGC0002226	0.44	NRP	acu-dioxomorpholine B, acu-dioxomorpholine A	Aspergillus aculeatus ATCC 16872
BGC0000348	0.44	NRP	ergovaline	Epichloe festucae var. lolii
BGC0001573	0.43	Alkaloid	dihydrolysergic acid	Claviceps africana
BGC0001775	0.41	Terpene	sespendole	Cordana terrestris
BGC0001264	0.34	Polyketide	betaenone A, betaenone B, betaenone C	Phoma betae
BGC0002157	0.34	NRP, Alkaloid	(-)-ditryptophenaline	Aspergillus flavus
BGC0001249	0.33	NRP	dimethylcoprogen	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001241	1.00	Terpene	ergotamine	Claviceps fusiformis
BGC0002232	1.00	Alkaloid	ergotamine	Claviceps purpurea 20.1
BGC0002157	0.67	NRP, Alkaloid	(-)-ditryptophenaline	Aspergillus flavus
BGC0002226	0.67	NRP	acu-dioxomorpholine B, acu-dioxomorpholine A	Aspergillus aculeatus ATCC 16872
BGC0001249	0.67	NRP	dimethylcoprogen	Alternaria alternata
BGC0000900	0.66	Other	ferrichrome	Aspergillus oryzae
BGC0001261	0.66	NRP	AM-toxin	Alternaria alternata
BGC0001573	0.66	Alkaloid	dihydrolysergic acid	Claviceps africana
BGC0002199	0.65	Alkaloid	nanangelenin A	Aspergillus nanangensis
BGC0002710	0.64	NRP	metachelin C, metachelin A, metachelin A-CE, metachelin B, dimerumic acid 11-mannoside, dimerumic acid	Metarhizium robertsii ARSEF 23

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 55951:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X) + (Ala - Phe - Pro) + (Ala - X - Pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CPUR_04073

Search NORINE for peptide: strict or relaxed

AMP-binding (309..706): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DAVFCVGPAK
Nearest Stachelhaus code(s):: Ala DLFFCGGPLK (79% 8Å match); Tyr DAQTIVGIAK (50% 8Å match); 6-clTrp DAAMCGGVAK (41% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (1396..1803): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLAGMGAMIK
Nearest Stachelhaus code(s):: Fo-OH-Orn DLWGMGAVNK (44% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2506..2906): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DITLVAGLIK
Nearest Stachelhaus code(s):: Pro DITLVAGLIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

CPUR_04074

Search NORINE for peptide: strict or relaxed

AMP-binding (309..706): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DAIFCGGPLK
Nearest Stachelhaus code(s):: Ala DLFFCGGPLK (91% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1396..1803): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DLVGMAAVGK
Nearest Stachelhaus code(s):: Phe DLVGMAAVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2507..2906): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DITLVAGLIK
Nearest Stachelhaus code(s):: Pro DITLVAGLIK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

CPUR_04083

AMP-binding (255..653): X

nrpys: D-Lya

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVFSVGLVMK
Nearest Stachelhaus code(s):: D-Lya DVFSVGLVMK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

CPUR_04085

AMP-binding (238..642): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIFLAGIIGK
Nearest Stachelhaus code(s):: Ph-Gly DIFLWGLLGK (44% 8Å match)
Stachelhaus code match:: 70% (weak)