BGC0002235 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002235 (Pyricularia oryzae 70-15)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	107,823	High	12,13-dihydroxymagnaporthepyrone/10,11-dihydroxymagnaporthepyrone/Unknown amide 8	PKS+NRPS:Type I

BGC0002235 - Region 1 - NRPS,T1PKS

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Location: 1 - 107,823 nt. (total: 107,823 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 107,824
Label		BGC0002235

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxygenase

MGG_08378: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

MGG_08379: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

MGG_08380: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

MGG_08387: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

MGG_08389: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MGG_08391: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

MGG_15927: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

MGG_15928: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Transferases: methyltransferase

MGG_08377: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Ligases: synthase

MGG_12447: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MGG_15097: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002235.2	1.94	NRPS, PKS	12,13-dihydroxymagnaporthepyrone, 10,11-dihydroxymagnaporthepyrone, Unknown amide 8	Pyricularia oryzae 70-15
BGC0002192.3	1.64	PKS	FR901512	fungal sp. No.14919
BGC0001268.4	1.62	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	1.55	PKS	ACT-Toxin II	Alternaria alternata
BGC0002191.2	1.27	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002538.3	0.84	PKS	chaetolivacine A, chaetolivacine B, chaetolivacine C	Chaetomium olivaceum
BGC0002539.3	0.81	other (other)	Chaetoglobosin P	Discosia rubi
BGC0000983.5	0.81	NRPS, PKS	cytochalasin E, cytochalasin K	Aspergillus clavatus NRRL 1
BGC0001264.3	0.78	PKS	betaenone A, betaenone B, betaenone C	Phoma betae
BGC0002546.3	0.77	NRPS, PKS	cytoglobosin X	Chaetomium globosum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002235.2	1.00	NRPS, PKS	12,13-dihydroxymagnaporthepyrone, 10,11-dihydroxymagnaporthepyrone, Unknown amide 8	Pyricularia oryzae 70-15
BGC0002192.3	0.77	PKS	FR901512	fungal sp. No.14919
BGC0001268.4	0.77	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.76	PKS	ACT-Toxin II	Alternaria alternata
BGC0002191.2	0.65	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002539.3	0.57	other (other)	Chaetoglobosin P	Discosia rubi
BGC0002538.3	0.57	PKS	chaetolivacine A, chaetolivacine B, chaetolivacine C	Chaetomium olivaceum
BGC0002230.2	0.57	NRPS, PKS	tolypyridone C	Tolypocladium sp. 49Y
BGC0002035.3	0.56	NRPS, PKS	ilicicolin H, ilicicolin J, 8-epi-ilicicolin H	Neonectria sp. DH2
BGC0002139.3	0.55	PKS	burnettiene A, preburnettiene B, preburnettiene A	Aspergillus burnettii FRR 5400

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 52353:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-Me-ccmal - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 54961 - 107823:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MGG_12447: mmal - X

PKS_AT (559..878): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 70.8%; Malonyl-CoA: 66.7%; CE-Malonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 74.0
Methoxymalonyl-CoA: 59.1
Isobutyryl-CoA: 42.9
Ethylmalonyl-CoA: 39.7
Benzoyl-CoA: 29.0
fatty_acid: 19.5
trans-1,2-CPDA: 13.4
Malonyl-CoA: 13.4
CHC-CoA: 12.2
Propionyl-CoA: 11.1
2-Methylbutyryl-CoA: 7.6
inactive: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

AMP-binding (3073..3479): X

nrpys: Orn, Lys, Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMAQLGGINK
Nearest Stachelhaus code(s):: OH-Orn DMENLGLINK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

MGG_15097: pk - X

PKS_AT (555..873): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 62.5%; Methoxymalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 67.9
Methoxymalonyl-CoA: 61.4
Ethylmalonyl-CoA: 47.9
Isobutyryl-CoA: 29.6
Propionyl-CoA: 24.1
Benzoyl-CoA: 23.6
2-Methylbutyryl-CoA: 17.9
trans-1,2-CPDA: 12.3
3-Methylbutyryl-CoA: 11.1
fatty_acid: 9.5
inactive: 8.2
Acetyl-CoA: 8.1
CHC-CoA: 0.0
Malonyl-CoA: -2.3

AMP-binding (3143..3560): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMSSVGGFCK
Nearest Stachelhaus code(s):: Phe DMSEVGCFCK (68% 8Å match)
Stachelhaus code match:: 80% (moderate)

Link to PARAS substrate predictor