BGC0002248 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002248 (Aspergillus flavus NRRL3357)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,terpene	1	43,216	High	flavunoidine	terpene+NRPS:Type I

BGC0002248 - Region 1 - NRPS,terpene

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Location: 1 - 43,216 nt. (total: 43,216 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

Terpene

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 43,217
Label		BGC0002248

Tailoring functions

Oxidoreductases: oxygenase

G4B84_005463: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Lyases: decarboxylase

G4B84_005466: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Ligases: ligase

G4B84_005468: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002248.3	1.92	NRPS, terpene	flavunoidine	Aspergillus flavus NRRL3357
BGC0001249.5	0.85	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0002164.2	0.69	NRPS	peramine	Epichloe festucae
BGC0001240.4	0.64	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000307.4	0.62	NRPS	AbT1	Aureobasidium pullulans
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001166.5	0.59	NRPS	HC-toxin	Alternaria jesenskae
BGC0000342.4	0.58	NRPS	enniatin	Fusarium equiseti
BGC0001475.4	0.56	terpene	aspterric acid	Aspergillus terreus NIH2624

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002248.3	0.99	NRPS, terpene	flavunoidine	Aspergillus flavus NRRL3357
BGC0001249.5	0.74	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.68	NRPS	AM-toxin	Alternaria alternata
BGC0002164.2	0.68	NRPS	peramine	Epichloe festucae
BGC0001240.4	0.65	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000307.4	0.64	NRPS	AbT1	Aureobasidium pullulans
BGC0000357.5	0.63	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001166.5	0.63	NRPS	HC-toxin	Alternaria jesenskae
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti
BGC0001475.4	0.62	terpene	aspterric acid	Aspergillus terreus NIH2624

Similar known clusters from MIBiG 4.0

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene	trichothecene	trichodiene	C15	C1-C6	none
sesquiterpene		acoradiene	C15	C1-C6	none

Genes with terpene domains:

G4B84_005464: T1TS

Domain 1 (4 - 307): Type 1 terpene synthase

Subtype: Trichodiene synthase type

Reactions:: FPP → trichodiene; FPP → acoradiene

G4B84_005465: T1TS

Domain 1 (6 - 320): Type 1 terpene synthase

Subtype: unknown

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Terpene

Predicted core structure(s)

For candidate cluster 1, location 0 - 43216:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

G4B84_005468: X

AMP-binding (32..423): X

nrpys: dmePip

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLFIGVGCK
Nearest Stachelhaus code(s):: dmePip DVLFIGVGCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate