BGC0002250 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002250 (Pyrenophora tritici-repentis Pt-1C-BFP)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	56,545	High	triticone D/triticone E/triticone A/triticone B/triticone F/triticone C	PKS+NRPS:Type I

BGC0002250 - Region 1 - NRPS,T1PKS

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Location: 1 - 56,545 nt. (total: 56,545 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 56,546
Label		BGC0002250

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase oxygenase

PTRG_04241: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

PTRG_04245: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

PTRG_04246: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

PTRG_04248: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

PTRG_04249: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

PTRG_04252: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

PTRG_04254: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Transferases: acylase methyltransferase

PTRG_04244: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

PTRG_04253: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002250.2	1.94	NRPS, PKS	triticone D, triticone E, triticone A, triticone B, triticone F, triticone C	Pyrenophora tritici-repentis Pt-1C-BFP
BGC0001563.3	1.10	NRPS, PKS	curvupallide-B	Cochliobolus pallescens
BGC0001268.4	0.80	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002192.3	0.67	PKS	FR901512	fungal sp. No.14919
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001254.3	0.63	PKS	ACT-Toxin II	Alternaria alternata
BGC0001924.3	0.43	terpene	ascofuranone	Acremonium egyptiacum
BGC0002205.2	0.41	NRPS, PKS	phomacin D, phomacin E	Parastagonospora nodorum SN15
BGC0002230.2	0.39	NRPS, PKS	tolypyridone C	Tolypocladium sp. 49Y
BGC0001264.3	0.38	PKS	betaenone A, betaenone B, betaenone C	Phoma betae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002250.2	1.00	NRPS, PKS	triticone D, triticone E, triticone A, triticone B, triticone F, triticone C	Pyrenophora tritici-repentis Pt-1C-BFP
BGC0001563.3	0.81	NRPS, PKS	curvupallide-B	Cochliobolus pallescens
BGC0001268.4	0.72	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002192.3	0.67	PKS	FR901512	fungal sp. No.14919
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001254.3	0.65	PKS	ACT-Toxin II	Alternaria alternata
BGC0001924.3	0.54	terpene	ascofuranone	Acremonium egyptiacum
BGC0002230.2	0.52	NRPS, PKS	tolypyridone C	Tolypocladium sp. 49Y
BGC0002093.2	0.51	NRPS, PKS	ilicicolin H	Talaromyces variabilis
BGC0001264.3	0.50	PKS	betaenone A, betaenone B, betaenone C	Phoma betae

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 51753:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

PTRG_04244: pk - X

PKS_AT (556..877): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 74.9
Methoxymalonyl-CoA: 62.1
Isobutyryl-CoA: 56.9
Ethylmalonyl-CoA: 54.6
Malonyl-CoA: 40.3
trans-1,2-CPDA: 26.6
fatty_acid: 26.0
Benzoyl-CoA: 24.6
Propionyl-CoA: 17.6
2-Methylbutyryl-CoA: 15.0
Acetyl-CoA: 14.9
3-Methylbutyryl-CoA: 13.5
CHC-CoA: 12.7
inactive: 0.0

AMP-binding (2918..3331): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DFDKFGSIAK
Nearest Stachelhaus code(s):: Phe NHDKFGSISK (71% 8Å match); MalAc NHDKFGSISK (68% 8Å match); Ser DFDTFGGILK (65% 8Å match); Tyr DFESFGNIAK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor