BGC0002259 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002259 (Cordyceps militaris CM01)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	30,543	beauveriolide I/beauveriolide III	PKS+NRPS:Type I	100%

BGC0002259 - Region 1 - NRPS,T1PKS

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Location: 1 - 30,543 nt. (total: 30,543 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 30,544
Label		BGC0002259

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002259	1.54	Polyketide, NRP	beauveriolide I, beauveriolide III	Cordyceps militaris CM01
BGC0002203	0.95	NRP, Polyketide, Other	beauveriolide B, beauveriolide C, beauveriolide D	Beauveria bassiana ARSEF 2860
BGC0002191	0.64	Polyketide	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001290	0.63	NRP	emericellamide A, emericellamide B	Aspergillus nidulans FGSC A4
BGC0002525	0.60	Polyketide	fusarubin, 1233A, 1233B, NG-391, lucilactaene	Fusarium sp.
BGC0001252	0.60	Polyketide	UNII-YC2Q1O94PT	Alternaria alternata
BGC0002155	0.59	Polyketide	nectriapyrone C, nectriapyrone D, nectriapyrone	Pyricularia oryzae 70-15
BGC0002515	0.55	Polyketide	solanapyrone A	Ascochyta rabiei
BGC0002194	0.54	Polyketide	epipyrone A	Epicoccum nigrum
BGC0002224	0.53	Polyketide	zopfiellin	Diffractella curvata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002259	1.00	Polyketide, NRP	beauveriolide I, beauveriolide III	Cordyceps militaris CM01
BGC0002203	0.84	NRP, Polyketide, Other	beauveriolide B, beauveriolide C, beauveriolide D	Beauveria bassiana ARSEF 2860
BGC0001290	0.67	NRP	emericellamide A, emericellamide B	Aspergillus nidulans FGSC A4
BGC0002191	0.65	Polyketide	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002525	0.64	Polyketide	fusarubin, 1233A, 1233B, NG-391, lucilactaene	Fusarium sp.
BGC0001252	0.63	Polyketide	UNII-YC2Q1O94PT	Alternaria alternata
BGC0002155	0.63	Polyketide	nectriapyrone C, nectriapyrone D, nectriapyrone	Pyricularia oryzae 70-15
BGC0001261	0.62	NRP	AM-toxin	Alternaria alternata
BGC0002515	0.61	Polyketide	solanapyrone A	Ascochyta rabiei
BGC0002194	0.61	Polyketide	epipyrone A	Epicoccum nigrum

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 30543:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Phe - Ala - D-Leu)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 29255:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Phe - Ala - D-Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CCM_01282

PKS_AT (559..878): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 58.3%; inactive: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 72.6
Methylmalonyl-CoA: 68.8
Malonyl-CoA: 46.4
Ethylmalonyl-CoA: 41.5
Isobutyryl-CoA: 35.0
2-Methylbutyryl-CoA: 26.9
Propionyl-CoA: 23.9
trans-1,2-CPDA: 17.7
fatty_acid: 16.2
Benzoyl-CoA: 15.9
CHC-CoA: 10.8
3-Methylbutyryl-CoA: 10.7
inactive: 0.0
Acetyl-CoA: 0.0

CCM_01284

AMP-binding (93..534): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SHGYVGAIVK
Nearest Stachelhaus code(s):: Thr DGMLVGAIVK (41% 8Å match); Leu DAWIVGAIVK (38% 8Å match); nohBza GLGLVGAIIK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

CCM_01285

Search NORINE for peptide: strict or relaxed

AMP-binding (590..984): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAYLMIGIIK
Nearest Stachelhaus code(s):: Phe DAYLMIGIIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2161..2551): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLGIVGGMIK
Nearest Stachelhaus code(s):: Ala DLGIVGGMIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3254..3647): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMHMLGVIIK
Nearest Stachelhaus code(s):: Leu DMHMLGVIIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)