BGC0002259 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002259 (Cordyceps militaris CM01)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	68,816	High	beauveriolide I/beauveriolide III	PKS+NRPS:Type I

BGC0002259 - Region 1 - NRPS,T1PKS

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Location: 1 - 68,816 nt. (total: 68,816 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 68,817
Label		BGC0002259

Tailoring functions

Ligases: ligase synthase

CCM_01282: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CCM_01284: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CCM_01285: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002259.2	1.48	NRPS, PKS	beauveriolide I, beauveriolide III	Cordyceps militaris CM01
BGC0002191.2	0.90	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002203.2	0.84	NRPS, PKS, other (other)	beauverolide Ba, beauverolide Ka, beauverolide Ca	Beauveria bassiana ARSEF 2860
BGC0001244.3	0.65	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.65	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.65	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002192.3	0.63	PKS	FR901512	fungal sp. No.14919
BGC0001268.4	0.61	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001290.4	0.60	NRPS	emericellamide A, emericellamide B	Aspergillus nidulans FGSC A4
BGC0001254.3	0.60	PKS	ACT-Toxin II	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002259.2	0.98	NRPS, PKS	beauveriolide I, beauveriolide III	Cordyceps militaris CM01
BGC0002203.2	0.80	NRPS, PKS, other (other)	beauverolide Ba, beauverolide Ka, beauverolide Ca	Beauveria bassiana ARSEF 2860
BGC0002191.2	0.75	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0000357.5	0.69	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001166.5	0.68	NRPS	HC-toxin	Alternaria jesenskae
BGC0001240.4	0.68	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 68816:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Phe - Ala - D-Leu)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 48406:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Phe - Ala - D-Leu)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 14465 - 68816:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Phe - Ala - D-Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CCM_01282: pk

PKS_AT (559..878): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 58.3%; inactive: 54.2%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 72.6
Methylmalonyl-CoA: 68.8
Malonyl-CoA: 46.4
Ethylmalonyl-CoA: 41.5
Isobutyryl-CoA: 35.0
2-Methylbutyryl-CoA: 26.9
Propionyl-CoA: 23.9
trans-1,2-CPDA: 17.7
fatty_acid: 16.2
Benzoyl-CoA: 15.9
CHC-CoA: 10.8
3-Methylbutyryl-CoA: 10.7
inactive: 0.0
Acetyl-CoA: 0.0

CCM_01284: X

AMP-binding (93..534): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SHGYVGAIVK
Nearest Stachelhaus code(s):: Thr DGMLVGAIVK (41% 8Å match); Leu DAWIVGAIVK (38% 8Å match); nohBza GLGLVGAIIK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

CCM_01285: Phe - Ala - Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (590..984): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAYLMIGIIK
Nearest Stachelhaus code(s):: Phe DAYLMIGIIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2161..2551): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLGIVGGMIK
Nearest Stachelhaus code(s):: Ala DLGIVGGMIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3254..3647): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMHMLGVIIK
Nearest Stachelhaus code(s):: Leu DMHMLGVIIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor