LOCUS       BGC0002260             17473 bp    DNA     linear   PLN 07-OCT-2020
DEFINITION  Annulohypoxylon truncatum culture CBS:140778 brasilane biosynthetic
            gene cluster, complete sequence.
ACCESSION   BGC0002260
VERSION     BGC0002260
KEYWORDS    .
SOURCE      Annulohypoxylon truncatum
  ORGANISM  Annulohypoxylon truncatum
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Sordariomycetes; Xylariomycetidae; Xylariales; Hypoxylaceae;
            Annulohypoxylon.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:13:07
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 17473
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..17473
                     /culture_collection="CBS:140778"
                     /db_xref="taxon:327061"
                     /mol_type="genomic DNA"
                     /organism="Annulohypoxylon truncatum"
                     /type_material="culture from epitype of Annulohypoxylon
                     truncatum"
     misc_feature    1..17473
                     /note="brasilane biosynthetic gene cluster"
     subregion       1..17473
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002260"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..17473
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(Terpene_synth or Terpene_synth_C or phytoene_synt
                     or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps
                     or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /subregion_numbers="1"
                     /tool="antismash"
     protocluster    1..12362
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[942:2362]"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      943..2362
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     cand_cluster    1..12362
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     gene            <943..>2362
                     /gene="braA"
     mRNA            join(<943..1145,1206..1335,1389..1407,1463..1929,
                     2000..2226,2281..>2362)
                     /gene="braA"
                     /product="brasilane synthase"
     CDS             join(943..1145,1206..1335,1389..1407,1463..1929,2000..2226,
                     2281..2362)
                     /codon_start=1
                     /gene="braA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: Terpene_synth_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Terpene synthase/cyclase metal-binding domain
                     protein (Score: 194.7; E-value: 4.3e-59)"
                     /gene_kind="biosynthetic"
                     /note="sesquiterpene cyclase; BraA"
                     /product="brasilane synthase"
                     /protein_id="QOE88883.1"
                     /sec_met_domain="Terpene_synth_C (E-value: 2.4e-22,
                     bitscore: 68.0, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPDIDNIWSSTTDAAESPVDERRILLKRAVGQKILVPSILSLMP
                     AWPSQVHPAVDEVNTEIDKWLPTVNVAEKKKAKHRARGNYAFLTAVYYPYCKETERLVV
                     IAKFLYWIFFWDDEIDTGGELTEDEEGTIQCCEETNKCVDDCLGPNPNYNPPPNSRGTV
                     EMFYPILRDFRAGLGPVSTERLRLELHDYINGVAKQQKVRQGERLPDPWYHFKIRSDDV
                     GVIPSITQNEYAMKFELPEYVRRHEAMEEIVQECTKLTVLLNDVLSLQKEFRDSQLENL
                     VLLFMNRYNLSLQAAVDKVLDLIREHYAICVAAEKRLPWSEDDEKLNDDIREYVRGCQR
                     LATGTAYWSYSCERYFKQTQVNDKWEVLLDLSYVE"
     gene            complement(<2625..>3890)
                     /gene="braB"
     mRNA            complement(<2625..>3890)
                     /gene="braB"
                     /product="N-acetylglucosamine transferase"
     CDS             complement(2625..3890)
                     /codon_start=1
                     /gene="braB"
                     /note="glycosyltransferase; BraB"
                     /product="N-acetylglucosamine transferase"
                     /protein_id="QOE88884.1"
                     /transl_table=11
                     /translation="MVPSVMEGAPQLGITSTDTSSAGVPPGLKLIPTDRLDRRTDEEIA
                     AWLQTRHPVTSDKNVWAFWHNGYTNMPPWVQRNIINWVRRLGPDWTVHLLDRVDGSATN
                     VSHYVDSSFFPECFNNNTMDGPTVGQHSGDLVRLPLLWLYGGIWIDAGSFLFRHVEDIC
                     WNKIEDPESPYEMAGFVIEMRPGVEVMLNGFIAAKRGNPFIKRWLEIFKKLWDGATNVQ
                     GFHKHPLLRHLPMLCPPIDKLNLPPQGLNVVMEQFTDYMSQIMSFERLRKLVDPSDGFN
                     GPEYYSNKMLLCSALQETFYFQLVTEWSGTKQFNLLSTKRKGEGVVKDENWHAAENFVH
                     DALANTATMKLSHGPPGALDSFLADLWDSEEHHGKDNEDGTFAAYLRYGSVHFDQTREM
                     VPIKMGWPDEEVLEAGVLEPKK"
     gene            <5081..>6849
                     /gene="braC"
     mRNA            join(<5081..5284,5352..5477,5539..5692,5755..6366,
                     6431..>6849)
                     /gene="braC"
                     /product="cytochrome P450 monooxygenase"
     CDS             join(5081..5284,5352..5477,5539..5692,5755..6366,
                     6431..6849)
                     /EC_number="1.14.14.1"
                     /codon_start=1
                     /gene="braC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 128.4; E-value: 6.2e-39)"
                     /gene_kind="biosynthetic-additional"
                     /note="BraC"
                     /product="cytochrome P450 monooxygenase"
                     /protein_id="QOE88885.1"
                     /sec_met_domain="p450 (E-value: 2.2e-46, bitscore: 146.9,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASLYLPTIWASTLTAATIFIVAVLSKWLRKPRSFDVPIVGAESG
                     DLNVLKARYVQEADALLREGYEKFKDAIFQVITPDGPRVFLPRKYAHDLKDYSRHEASG
                     MKALADRHIGHYTTIDHESDIMLGAIKIDLNRNLGTFVGDVEHEVAFCFETQFPACDDW
                     TPIDLHDKLLRVVAQASARIFVGYPMCRNEEWLECSTKFALDVMTGGEKLKQWHPYLRP
                     IAQYFVPEMTRIRGDHQRALELLLPELNRRLAEPADPDSSPHNDMIQWMQDRARKTGDN
                     SFDNKELANLQMLTATAAIHTTRLAIIHALYDLAARPEYVEPLRKEILEATKDSNGVLQ
                     KQHLTQMKMLDSFMKESQRHSPPSVATYQRKAMIPITLSNGFHIPAGTIVQCNTNILDE
                     TPPDWGDPHAFDGFRFYKLRNRTPDDINKFQFASPTYDSMQFGFGKDACPGRFFASNQI
                     KIILAYILSHYDIKFEDSVVGRPKNFMFEVNVLADPTKMVLFKKIR"
     gene            complement(<7367..>8228)
                     /gene="braD"
     mRNA            complement(join(<7367..7805,7859..8112,8184..>8228))
                     /gene="braD"
                     /product="hypothetical protein"
     CDS             complement(join(7367..7805,7859..8112,8184..8228))
                     /codon_start=1
                     /gene="braD"
                     /note="BraD"
                     /product="hypothetical protein"
                     /protein_id="QOE88886.1"
                     /transl_table=11
                     /translation="MSSDLVSKGMAQVFTGLGILLGAGRISSETHEEIMALLSADPGSN
                     TGIMTGTNTGSTSIIPTQSKRVDMGDIPAGLPRPGKETPVSIQSHDLLGLGADLSTEAV
                     QPPETFRAQASPSAPSKELKIICPWWLTDGYSCREHDQGKCPFYHDNVAGGVKHPLICH
                     FWADGGRCTKSQKDCRFAHYPAPHRVTAPMPSKKKSKKLRSSVADDASHPDLGKARRHD
                     PRDDEQNDEVWRNQGRARPGQEW"
     gene            complement(<11900..>16838)
                     /gene="braE"
     mRNA            complement(join(<11900..13019,13150..13284,13419..15439,
                     15613..16566,16623..>16838))
                     /gene="braE"
                     /product="ABC transporter"
     CDS             complement(join(11900..13019,13150..13284,13419..15439,
                     15613..16566,16623..16838))
                     /codon_start=1
                     /gene="braE"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 134; E-value: 1.1e-40)"
                     /gene_kind="transport"
                     /note="BraE"
                     /product="ABC transporter"
                     /protein_id="QOE88887.1"
                     /transl_table=11
                     /translation="MLFSACADTNDQSLGPNVLGCRGDFDFTVKFEQLCFSLTPAAIFI
                     LASPWRVAHLVRKPTIVGAPLLRLAKLGVLVSYASLELSQLILITVLPFGASGIDIISS
                     ALRLAAALCMVGLSYFDHSKSPRPSIFLSAYLFFTLLFDIAQARTYWLASSTRPEIAFT
                     AIFTAALAMKIAMLLLEAQRKTKWVAWDSKDHSPEETSGIYTLGVFSWLNKLFFDGYHK
                     TLGIRDLYPLDQNLAATHLSERFSRHINEAKRKGHEIGLMEALAKTLYVPMILPIPAKL
                     AAIGSFFCQPLFISSLTSRLSQSEPVPANIGYGFIGASICIYSVIAISQSLYWYFQQRL
                     LYMMRACLATAIYTKTTEARAADEDENASLTLMSIDIERIMKGSLYMHELWGNVIEVAL
                     SAWLLYNLLGVAFIAPIVVVCICVGGVSFFMRFMGDSQRNWMAGIQKRVGLTSSVIGNM
                     KNIKISGLTSPISRFVEKLRVDELQAGSNFRLLMLTCSVFAYIPLLLSPPITFGVARRS
                     LDATKLFTSLSYLLLMSTPLQNLLETLPQMAAAVACLGRIQKFLQGEGRDDYRIFLAGS
                     RRDPEKPSLDQLDKSPAVAIKDGSFGWKPDKMVLNNLDVEIPRGSLTIVVGPIASGKSS
                     LCKALLGEMPHSQGTVTIATKFSCVGYCDQTPFLSNGSIRDNIIGYSPFDAQRYAEVVD
                     GTMLGIDFETLPEADRTNIGSNGITLSGGQKQRVSLARCLYLQSDLLIMDDVFSGLDAD
                     TEDQVFQRVFGANGILKRRQATVVLCTHSVRHIPSATHVIALSTDGTVVEQGTFGDLVA
                     NQSYIHSLGVKAPSTSQADSEKIESDDSAIEPQINLIERAPTETPEVGVNDKSRLSGDS
                     AAYIVYMKSMGTMLPIAIFTSGLLYGFFYNFPTIWLTYWSADAVATNPSHSFGYYAAIY
                     AVLEVCAMLSLIWLGVLLYITVLTRSGVSLHHAALRTLIHAPLRFFTTTDQGIITNLFS
                     QDLSLIDNELPSALLNVIYMVFVGIGQAAVIASSSPYLAISYPFLFGMLYVVQKFYLRT
                     SRQLRLLDLEAKSPLYTHFLDTSKGIVTLRAFGFVSEDRAKNAFLLDTSQRPAYLLAMI
                     QQWLHFVLNVVVAIIAVMLTSLAVRLRSNSGFTGASLVTLMSFGEMLSGVVIYYTALET
                     SLGAISRLKAFDKAAKTETKDGEDIVPPEEWPPRGEIILNNVSASYEYELPLFLSIRLT
                     TNELSSNDTQPETPTLALKNLRLRIRPGEKIAICGRTGSGKSSLISLLLKLLDPIDETL
                     DCVNIDNTPLSRIDRVTLRQRIIAIPQDIVFLPDGSTFQENLDPSNVSTAADAQAVLEA
                     VDLWDFVRDKGGLEAGMTVSNLSQGQRQLFSLGRAVLRRRIRARSLGLGGGGSEGGILL
                     LDEVSSSVDRETEKAMQEVIRVEFREYTVVAVSHRLDIIMDYDRVVVMEKGEIVEEGNP
                     ARLVEEPGTRFGELWSVGGN"
ORIGIN
        1 aacgcgaggt aatatcaagc aaggcgcagc gataataatc ctctatctaa tgaaataagt
       61 tgctctgtaa cgttgatctc tataattgat acgagtgctt ccatctatat accaatgaag
      121 tgtttttaac ccttgtctgt cttttctggg ccttacctgc ttggtggggt tatgctatga
      181 agcttactat cttccgtaat gataagagta tactcagctc cttagagttc ttagaactcg
      241 ggaggattct ttcgggtaaa cctgttaggc ttatagcgat gagaccgact catcctacag
      301 tcaccgtagg tctcgtaggc ctacgaaatt caaccatcca ttggatccca atcttggtcc
      361 cttggaggcc cagccctccc gaaggatcgt cgccagcttt tctctcacct ccatctacaa
      421 tgcagctttc ggaaaggttc cgatgaatcg aggtaggttc gtgtacatag ccggttacat
      481 ctgatgacac aacagcctcc tttggaccaa gacagaccca caggtacctg atttacggta
      541 gcaggaggca aaccccatag ctgcagaact cacgaatagc tgacccatca tgctctttgg
      601 gccacgggag cctcttggtt accagtacac tcatgcacat gtacttaggt tgcactcggt
      661 tgaaggatgc agccttgatt gtaaccgaat atgtcacact tgggatgaca ctgcctagtg
      721 atgtgttggt ctaaatcttt ctgagttaga caggattgcc tgcactaaca ctaaatatcc
      781 caagggccgg ttatgtctca cgatgagatc gagagcttat atagaggccc acacgggccc
      841 ctaggttgat gtgcgtaggg attctccctg gaaagaaaga ccaattcata cgacgatctc
      901 ctgccgttgg ttacctatct ggatttactt atcacttaca ttatggctcc agacatcgac
      961 aatatctggt cgtcgaccac agatgcggct gagagccccg tcgacgagcg cagaatcctc
     1021 ctcaagagag ccgtgggcca aaagatccta gtccctagca tcttgtctct aatgccggca
     1081 tggcctagtc aagtgcaccc agctgttgat gaagtcaata cggagataga taagtggctc
     1141 ccgacgtaag ctaaatttta caatcctcag attataggca attcttttct aatgatataa
     1201 taaagtgtca atgttgccga gaagaagaag gcgaagcacc gcgctcgtgg gaactacgca
     1261 ttccttacag ccgtgtacta tccctactgc aaggagacgg agcgtcttgt tgtcatagca
     1321 aaattccttt attgggtacg ttttctaggt ctattgtgaa tccaaatgac actgacaatc
     1381 cgacatagat attcttctgg gatgatggtg aatataacgc cacgagtaaa agctttgtag
     1441 cggtagactg acttctaatt agagattgac acgggaggag agcttaccga ggatgaagag
     1501 ggtactatcc agtgttgcga agagacgaac aaatgtgttg atgactgtct cggccccaac
     1561 cctaactaca acccccctcc aaactcccgg ggaacggttg agatgttcta ccccatctta
     1621 agagatttcc gagcaggtct tgggccagtt tccacggaaa gacttcgact cgagctccat
     1681 gactacatca atggtgtcgc aaaacaacag aaggttcgtc aaggggaacg cttaccagac
     1741 ccctggtatc acttcaaaat tcggtcggat gacgttggag tcattccaag cattacgcag
     1801 aatgagtatg ccatgaaatt tgagttgccc gagtacgttc gcagacacga ggcaatggag
     1861 gaaattgttc aggagtgcac caaactcacc gtccttctta atgatgtatt atccctacag
     1921 aaggaattcg tatgttctac accccctgaa ccggagcacg agtccacacg gctaacagtg
     1981 aaacccccaa ccaccacagc gagattcaca gctggagaat ctcgtcctcc ttttcatgaa
     2041 caggtataat ctgtcacttc aagccgccgt cgacaaggtc ctcgatctca ttcgagagca
     2101 ctacgcgatt tgcgtcgcgg ccgagaagag gcttccctgg agcgaagatg acgagaagtt
     2161 gaatgatgac atcagagagt atgtccgcgg ctgccagaga ttggctaccg gtactgcgta
     2221 ttggaggtat gtgctgcgac gctgaaacga accaatagca ggttgactaa ttgatgacag
     2281 ttactcatgc gagagatact tcaagcaaac gcaggtaaat gataaatggg aggtactatt
     2341 ggatttgtca tatgtggaat gattatttcg aggcgcaatg ccaatgtcag catttccggg
     2401 gggcattttg acacgtcata agaatagtgt atttttattc cctacctaga tacatagttc
     2461 ctcatgtttg cccctcgagt attgaaaggt gcctaaactg atcgacctta ttccttcttc
     2521 ggcatgtagt tagagagaat atcattccaa ttgaacacaa aaaataattt tcacacaggt
     2581 tttatctata tttatcccat tttctaaaga ataaatgccc ctttttactt cttcggctcc
     2641 agaaccccgg cctccaacac ctcctcatca ggccagccca tcttaatcgg gaccatctcc
     2701 cgcgtctggt cgaagtgcac gctcccatac ctcaagtaag ccgcaaaagt gccgtcctcg
     2761 ttatccttac cgtggtgctc ttccgagtcc cacaggtccg cgaggaacga gtccaaagca
     2821 ccaggggggc cgtgcgacag cttcatagta gccgtgttgg cgagcgcatc atggacgaag
     2881 ttctcggcgg cgtgccaatt ctcgtccttg acgacgccct cgcccttgcg cttcgtcgat
     2941 agcagattga actgcttggt gcccgaccat tccgttacga gctgaaagta aaatgtctcc
     3001 tggagcgccg agcacaggag catcttattc gagtagtact cgggaccgtt aaacccatcc
     3061 gacgggtcga cgagcttgcg tagccgttcg aagctcatga tttgggacat gtaatcggtg
     3121 aactgctcca tcacgacatt aaggccttgc ggtgggagat ttagcttgtc tataggcgga
     3181 cagagcattg gtaggtgtcg cagtaaggga tgcttgtgga agccctgaac attcgtcgcg
     3241 ccgtcccaga gtttcttgaa gatctcaagc cagcgcttga taaatggatt accgcgcttc
     3301 gcggcgataa agccgttcag cataacctcg acccctgggc gcatctcgat cacgaaaccc
     3361 gccatctcgt acggagactc ggggtcctcg attttattcc agcaaatgtc ttcaacgtgg
     3421 cggaacagga atgaaccagc atcgatccag atgcccccgt aaagccagag caaggggaga
     3481 cgaacgagat ctcccgaatg ctggccgacc gtagggccgt ccatcgtgtt gttgttgaag
     3541 cattcgggga agaatgagct gtcgacataa tggctgacat tcgtcgcaga tccatctact
     3601 cggtctagta aatgcacagt ccaatccgga cccagtctgc gaacccaatt gatgatgttc
     3661 cgctggaccc agggcggcat gtttgtgtac ccattatgcc agaacgccca gacgttcttg
     3721 tccgacgtga cgggatggcg agtctggagc catgcagcga tctcctcgtc agtgcgccgg
     3781 tcgagcctgt ccgtcgggat caatttcagc cctggaggca ctccagcgct gcttgtgtct
     3841 gtggacgtga tacccagctg tggggctccc tccattaccg aaggtaccat cgtccctcgg
     3901 aagcggaggc ggctacgaat caatttcaga aatgatgttg tcaccgcagc gcttgttacg
     3961 tgagacctgt cacacttccc attgccgcac aagggcacgg ggtacgaggc ccgaggacgg
     4021 aaaaggcatc ctacataaat attccgaaaa tttgcatctt cgagacacta aaagcgacac
     4081 ttacgtcgcc gtaatgcgga atgctggggg cgcggcggac gtgaagaccg acaggttcca
     4141 gttagccatg aaccaacccg gagatgtcgt agtcgcctag gaccgcgagc aggccattta
     4201 gtccgctagt ggcgttccca ttttcacccc ttgaccccgc atgagaacca tgtcaacaag
     4261 ggatcggaat ggcaactgag gtacatgacc accttactat gtagttatta taggaaatct
     4321 attgcgacat gcgcctatgc ctgggaagta atcgtaagga tcgttgttag tgacaggtta
     4381 agtgtacatc gagaatagtt gacacttagc aaactgccaa gaagcgactt cgtgttatta
     4441 tcttcctttc gaacgcagtg acataccgag cctagggcat tgatgtaggt acctacatag
     4501 tagaattcta aaattcatgc ggcctacatg gcgaccgggc tctctcccga cccaggctta
     4561 cgggttcgcg cgagataaca gccctccaac tctcgattgg ggggccgcag tggtggtggt
     4621 gggggatcgt ctgcccatgc tcatacgtgg cacggcccaa ggtcctgcag gcggagttgg
     4681 tcagcggagg cacatcatga tattagtgga ggattccgcc taaggcatgt gacgttgcag
     4741 cgccgcaacg tgctcaccgg ggattcttgt ctgccaaggt agtagggcga tagcacgcta
     4801 ctgcatggac taggtaggct ggtaggatca acgatccttc agcggacagg gaccctgaat
     4861 atcatttgct taggtgagat gacggcgagg ctttacccac gcgggggctc ctccgatgcg
     4921 tgtatccttg cctgcttatt tgtagatcgg cttattaggt ggctctcgta tgtgagaact
     4981 tggcaatggg agtctcgaga cgggcagcgc aggcagattg caaatccata ctcttcattc
     5041 cctagctcac gtcgatgtaa ttttgactac ctaggtagag atggcatctc tttacttgcc
     5101 gacaatttgg gcgtccacgc tcacggcggc gaccatcttc atcgtcgctg tcctctccaa
     5161 gtggcttcga aaaccgaggt cgttcgacgt cccaattgta ggtgctgagt ctggagatct
     5221 caatgtgctc aaggctcgat atgtgcagga agcagacgcg cttttgcgtg aaggatatga
     5281 aaaagttgag tttccgacat ggcggcttag gtttggcgcc gacgaaatga actccggctg
     5341 attcatttta gttcaaagat gcaatcttcc aagtcatcac tcccgacgga cctagagttt
     5401 tcctaccaag gaaatacgcg cacgacttga aagattatag tcgtcatgaa gcgagtggga
     5461 tgaaggcgtt ggctgatgtg agttttcttt cgggcggggc tcgactgcgg tgttgatgat
     5521 taacggtatc atttctagcg tcatatcggt cattacacta ccattgacca cgaaagcgat
     5581 atcatgctag gcgcgatcaa aatcgatctc aacaggaatc tgggcacctt cgtcggagat
     5641 gtcgagcacg aggtagcatt ctgcttcgag actcagtttc cggcatgtga tggtacagcc
     5701 tttcaatcac aaactaaatg ccttagaagt gaaaagtgct aacggatttg acagattgga
     5761 cgccaattga cctccacgat aaactcctcc gcgttgtggc acaggcttcg gctcggatct
     5821 tcgtcggtta tcccatgtgt cgaaacgagg aatggctaga atgcagcacg aaatttgccc
     5881 tagacgtcat gacagggggc gagaagttga agcaatggca tccgtatcta cggcccatag
     5941 ctcaatattt cgtgcccgag atgacgcgaa tccgcggaga ccaccagcga gctctcgaat
     6001 tgcttttacc tgagcttaac agacgactcg cggaacccgc agatccagac tcgtcgcccc
     6061 acaatgacat gatccagtgg atgcaggatc gcgcgcggaa aaccggagac aactcctttg
     6121 acaataagga gttggctaac ctccagatgc tcaccgcgac agctgccata cacaccacgc
     6181 gtcttgctat tatccatgct ctgtatgacc tcgcagctag gccggaatac gtggaacctc
     6241 tccgaaagga gatactagaa gcgacgaagg acagcaacgg cgtccttcaa aaacaacatc
     6301 tcacgcagat gaaaatgcta gacagcttca tgaaggaatc ccaaaggcat agtccgccct
     6361 cggtcggtaa gttgatctaa attgcttaac taggtaccga atggtcattt catattgatg
     6421 gattacatag ctacatacca acgcaaagct atgatcccta tcactctgtc gaacggcttc
     6481 catatccctg cgggtactat cgtgcagtgc aataccaaca tcctggatga gaccccaccg
     6541 gattggggag accctcacgc cttcgacgga ttccgattct acaagctccg taatagaacg
     6601 cccgatgata tcaacaaatt ccagtttgca agtccgacgt acgatagtat gcaattcggg
     6661 ttcgggaaag acgcatgccc aggtcgattc ttcgccagca accagatcaa gattattctc
     6721 gcctacatcc tctctcatta tgatatcaaa ttcgaggata gcgtcgtcgg aagacctaag
     6781 aactttatgt tcgaagttaa cgtcttggcc gaccccacga agatggtgtt atttaagaag
     6841 attcgatagg attgtaggta gtaaaggata taatagtatg ctactaacac aacctcaata
     6901 ttttttgcat cttagtggat tctcatttta ttgtgactgc tagtttcttg gggatagcta
     6961 gctcctattc cattaaccca ctacgacaaa ggccgtagtg tgagtttcga ttttatttgc
     7021 gttaaggccc attcaattcg gatttagtgt ctacctcagc aatttcgctc tcggtagata
     7081 agtcggtatc aggtaaggag ggaacgtttc gagatggttc tttctaggtt ataaccggga
     7141 acgaatttac caatgggcat catcttgctg aagagccaaa ggcattagcg gaaacaacgt
     7201 tgggtccctt cgagtgagga tcgggtgcgg agggacaccc cattaggggc catcaagccg
     7261 aataatagtg aaaaaagata agggaggtgt ggtatttaat gagacacagt actgaaactc
     7321 atcatcggtt gccgctcagc aaccaattct tagagcgaga gccattctac cactcttggc
     7381 cgggacgagc acgaccttgg ttgcgccaga cttcatcgtt ctgctcgtca tctcgtgggt
     7441 cgtgacggcg tgccttgccc aagtcgggat ggctggcgtc gtctgcgaca gaagaccgaa
     7501 gcttcttaga cttcttcttc gatggcatgg gagctgtaac ccgatgcggg gccgggtagt
     7561 gcgcaaagcg acagtccttc tggcttttag tgcaccttcc tccatctgcc cagaagtggc
     7621 agattagagg gtgcttaaca cccccagcga cgttgtcgtg gtagaaaggg catttgccct
     7681 ggtcgtgttc acggcacgag taaccgtcgg taagccacca cgggcagatt atcttaagct
     7741 ccttagaagg agcactaggg ctggcctggg cccggaaagt ctcgggaggc tgcactgcct
     7801 cagtcctgat caaggtcaac aaatgtccat tagctatgaa gactggtgaa gaacttacga
     7861 taggtctgct cctagaccca aaagatcgtg cgactggatg gagacaggtg tctcctttcc
     7921 gggacggggc aatccggcgg gaatatctcc catgtccacc cttttgcttt gggtgggaat
     7981 aatcgaggta gaccccgtgt tagttcccgt cataataccc gtgttggagc ccgggtcggc
     8041 actgagaaga gccatgatct cttcatgagt ttcgctggaa attcgaccag cgccaagtag
     8101 aattccaagc ccctatagga agacgtcatt agcttttatc ttcattgggt atgtggcagt
     8161 gttgatagat gggcaagact tacagtgaat acttgggcca tgcccttgct aactagatcg
     8221 gaggacatct tggtaatttc cgttaaacta caatagtcct tcaaggtaaa tatgttgcag
     8281 tgtatgataa actgcaagga ggatactata gggaaaagtt attagtggag atcaatctta
     8341 ggtgttggtg ataatgaacc cagagagact gttgtgtgtt ggacaggaag aggttgagaa
     8401 tgcgcagggc ttgtgggatt aaataggttt tcctaccttt ggctggtcgg agcggaagat
     8461 aggtgagtta gtagtggcca cggtaaaagt taggtaatgt acctaaccgg caggcccgac
     8521 cgaggaggga aatggattat gagtaatgtc caccgggtaa ggaatggaaa gaacgaagtc
     8581 gaagtataag agtgagtgat gaagtgatgg agtgagtggg tggatgggca agaagatata
     8641 tgttgaacgt tatgagtcga gaatgaaagg cacaccgccg acgatagtgt gcggtggata
     8701 ttatgcttcg ggtagaaatt gaatgtcctc agaagttatc cacgtagaca taggaagtta
     8761 gattccgtgg tcagtgttat aggattaacg gaatagaata aatacctttt cttaatgaag
     8821 acttggttag cagtctaagt tctttgtggt gaccgtttgt tatataggtt cggtctggag
     8881 ctgaattgca atagagtgct ggagctcgga tcgcttaaat accagtgttc tacaacgaat
     8941 gactaatctg agttgactcg ttcgtcgaac agcaggaaca gttctatcgg tagaacttta
     9001 ttcatactat attccccaag aaatgtgacg atctgccggc tcctgcacat gtttgcacgg
     9061 aagattaagc tttggggaag caaggtagta taagcttcga acagttacca gctgtgcacg
     9121 gacgattgag ccggttgagc taacgcaaaa accattcagt ggtccgtgta atatgacatt
     9181 atctctccga gtcaagagct taatcccttt ttgtaaggaa tagactctat ttgcatacag
     9241 ataccaaggc aagcacgatg gtgacttagc tttaaggtta tgagcaagaa aaaaaaatat
     9301 tcacaccagg agtagtcacg gtatatatca tctgaaaaaa ggaagaaaag tatgaaaacc
     9361 gttactatcg aggcagtcgc caatgagctt aattacccaa agaggaaaga tcagcggcga
     9421 tttccataac taaaagggaa agaggtgttt ttgattctca tcttcctatt ctgcagctac
     9481 ttgtatagtg agataacccc cctttactag cattgaagta agctattcca accaacatta
     9541 ggtatatata acattgccca gataaaaagt ttgattatga gcatataata agtattacat
     9601 tttatgacgt caaagttgaa cattcaggtt tggactactt gattgaagca atttaagact
     9661 tgttcaggtg ctcactcaag taagtgttat tggaaaagct tgacagctta ctcacttgaa
     9721 cagcgtaatc aagctgccat ttgggagatg acggatgact gaaatgtagt tgatcatacc
     9781 gcgattgtgc gaatagacgt ttacacgaat gcgttcaagt gtgttgagta tcaaacacga
     9841 gatgtcctaa actgatgaat aaacaatagt atgagataca ttgtaaatgg atgcagttta
     9901 aggtgtcttg caacgtagaa actgatatta ctgcttggaa ccccaacaca gtacccggag
     9961 ttgttgaact tttaacaaat taaattttag ttgttcttaa tgctagaaga ttgctgcata
    10021 agggggagtt tattcaaagg gttttgtaga catgatctgg gtaaaggtat atcaaaaggc
    10081 tatggatctt gtgacaatta aggaccttgt aagtaagctg ccgatatgta tccatgatta
    10141 tagtactatt ttgaagaata aagatagtgt ggcctgatag tttatagagc tgcacgattg
    10201 attattgcta tagtagaatg cagacgtgcg atagccccgg taaaatttat aaggatatcg
    10261 aatagattat gcgttggctg tgggtacata atatctaggc caagactgtg tggtggtaca
    10321 attcatgaag atattgacag ctatggcgtc gcggatacag gtgcgaatta tttttatata
    10381 tgatggcact gattattctt tgctactagg tacggtatga ctgaaacctg caatgatgtc
    10441 taggaaatat gatggatcac tctcgactgg gtaagtgtac ggaggcaagt aaattggtgc
    10501 gaaatgacag atcataaatg atagatctaa tgcgtcaaca tgtaaactgt acctacaagt
    10561 aggtacataa atacacgtgt aggttgaggc acctcaaggg tgcctagggg catttggata
    10621 cttagggcag ttgttttcag tgctattttt tcccccaagt aaaacgaaaa ccctcgacta
    10681 cagcacaaaa aatccggggt aagccaatcg gaaaattccc cttggcccaa aaccgcccag
    10741 atcgggacta gcgcaatcac tccacgtgct gcacggctga tcccgcaacc ccacctgcag
    10801 cctacatatt cccggggaca gggttgtttt cttttttcct ctttcttcct tctttcatca
    10861 tgccgcacac acaaaaaaat ccacaagcca gtcgggccaa actttcgaag gtcgccttcg
    10921 tcccaaccta tcggtctctc gtaggcccct agattgttca gttaagattc cactcaaagg
    10981 ccgcgggggg aacactcgcc tcgcagccat ggggccaatc tttgtatttc actgctcatg
    11041 ccggcctttc ttttttctct ctatggcgtc gcatgaagga tatgcaatca ttttctcgcg
    11101 ccgctcgttt tttcttaggc gtgagatttt gttgcatttc ctagggtaac gcaccacatg
    11161 cagaaaaaga tgcttcgcgc tcactttccc catgtgcgtt gctcggcagt ggcgccgatg
    11221 cgtgacaggt cgtctcctcc cctcggagcc ggctagccca cttgcgcagt cactcggatc
    11281 ggttggtcag gggactccgt gaaagccctc gggttaggtt acagattgta tcatatgctg
    11341 gttcaaggca acgggggggc acccttggcg catttacagc tggtttgacc ctaacccttc
    11401 ctggctttct aaattcccat acatcgttgg atatctcccc agttttgact atagtgatgt
    11461 tcggcactgt tggacgagag gggcttgcgg ggtttggata tgaccttact gcgtctattt
    11521 accttgtcta cctgcctcgg tgggtaggtg gggaagtaga tgcaggccag gaaccttctt
    11581 gtggcttctt tcttagtgac gtctagctgg ctttggattt ttatttttat ttttggaacc
    11641 ccgggttgca tgcaacccat tcatttttct ttttcatcac tgacttcagc tagctgggga
    11701 cgtggagaag aagacgctca attgcagttt tgaggttgtt tactttcacg gttgctaggt
    11761 tgcgtctgtg gcgtggtccg tcacgacgca actctagtgc gtacctacct aggtacgtag
    11821 taatgcttac ctgatccatc taattattac caatgttcac aggattattc acccaattta
    11881 cgaacaccga gatcaagatc tagttcccac caacgctcca caattctccg aatctagtcc
    11941 ccggctcctc aactagccgc gccgggttac cctcctccac aatctcgccc ttctccataa
    12001 cgacgacgcg gtcgtaatcc atgataatat ccaagcgatg gctcactgcc accaccgtgt
    12061 actccctaaa ttcgacgcga atcacttcct gcatcgcctt ctcggtctcc ctatcgacgc
    12121 tcgagctgac ctcatcaaga agtagaattc cgccctccga tccaccgccg ccgagtccga
    12181 gactgcgcgc ccgaatgcgt ctgcgaagca ccgctcggcc gagagagaat agctgtcgct
    12241 gaccttggct caagttgctt accgtcatgc ctgcctcgag gccgccttta tcgcggacga
    12301 agtcccagag atccaccgcc tcgagaacgg cctgagcatc cgctgccgtg gagacgttgg
    12361 agggatcaag gttctcttgg aacgtcgagc cgtctggaag gaagacgatg tcctggggta
    12421 tggcgatgat gcgctggcga agcgtgacgc ggtcgatacg actcagcggc gtattgtcaa
    12481 tgttgacaca atcgagtgtt tcgtcaatcg gatcgagaag tttcaataga agagaaatga
    12541 gagatgattt tccgcttcca gtacgtccgc aaatggcgat tttctcccca ggacgtatcc
    12601 taagtcgaag gttcttcaat gctaaggtcg gcgtctcggg ttgagtatca ttgctactga
    12661 gttcattagt tgttaatcta atagacagga acaggggtag ttcatactcg taactggctg
    12721 aaacattgtt taggattatt tctccccgcg gcggccactc ctccggtggg acgatatctt
    12781 ctccgtcttt tgtctccgtc ttcgcggcct tatcgaaagc tttgagccgg ctgatagcac
    12841 cgagagaagt ctcgagcgcc gtgtagtaaa taactacgcc agataacatt tcaccaaacg
    12901 acattagcgt gactagagat gcaccggtga aaccggagtt ggaacggagg cgaacggcga
    12961 gcgacgtgag catgacagcg ataatggcga ccacgacgtt gagtacaaaa tgcagccatc
    13021 tgtatagagt gtcagtgtct tgtaagccga gtgacagtat tcaatgaacg tttggatata
    13081 agggctgatg aattggaacc tgactaggtc gctaacaagc aaaagaattg aagattagaa
    13141 ataacctact gttggatcat agctagaaga tacgcaggtc gctgagatgt atctaagagg
    13201 aaggcattct ttgcgcggtc ttcgcttaca aaaccaaagg ctcgaagtgt tacaataccc
    13261 tttgaagtat cgaggaaatg tgtactacat agtgttagcg aagctattga atggtagaga
    13321 acccaatagg tgggtaggca tcaggcgatt ccggctagaa atccagcctg gtcgtctagg
    13381 aaaaatgggt cgccgattag gggtaatatt ccacttacta cagtgggctt ttcgcctcca
    13441 ggtctaacaa tctcaactgc ctcgaagttc gaagataaaa tttctgaact acatatagca
    13501 tgccaaatag gaacggataa ctaatcgcta ggtatgggga tgatgatgcg ataactgcag
    13561 cttggcctat gccaacgaac accatatata taacatttag cagggcactg gggagctcgt
    13621 tatcgatcag acttagatct tgagaaaaaa ggttcgtgat gatgccttga tctgtcgtag
    13681 taaagaacct aagcggcgca tggatgaggg ttcgcaaagc ggcgtggtgg agggagaccc
    13741 cggaccgagt aagtacggta atgtagagca aaacaccaag ccatatcagc gatagcatgg
    13801 cgcaaacctc aagcacggcg tatattgctg cataatatcc gaaagagtgg gagggattgg
    13861 tggcaacagc gtcagcggac caatacgtaa gccatattgt cgggaaattg tagaagaagc
    13921 cgtagaggag cccggaagtg aatatagcta tagggagcat tgttcccatg cttttcatgt
    13981 atactatata ggctgctgaa tcgccactga gcctggattt gtcatttact cctacttcag
    14041 gtgtctccgt tggagctcgt tcaattaagt ttatttgagg ttcgatagca ctgtcgtcgg
    14101 attcgatctt ctcgctgtcg gcctggcttg tcgacggcgc cttgacgcca aggctgtgaa
    14161 tataactttg gttagcgaca aggtcgccga aggtcccttg ttcgacaaca gtgccatcgg
    14221 tgcttagagc gataacatgc gtggcggacg gaatatgacg aacggaatgt gtacatagga
    14281 caaccgtggc ttgtcgtcgt tttaggattc catttgctcc aaacacacgt tggaacactt
    14341 ggtcttcggt gtccgcatcc aagccgctga aaacatcgtc catgattaag aggtcggact
    14401 gcaagtagag acaccttgct aacgatacgc gttgcttctg tcctccagat agggttatcc
    14461 cgttgcttcc aatgtttgtc ctatctgcct cgggcaatgt ttcgaagtca atacctagca
    14521 tagttccatc gacgacttcg gcataacgct gcgcatcgaa tggagaatag cctataatgt
    14581 tgtctctaat actgccgttt gataggaacg gtgtttggtc acagtagccc acgcatgaaa
    14641 acttagtagc tatcgttact gtcccttggc tgtgaggcat ttctcctaga agtgctttac
    14701 aaagcgatga ctttcctgat gcaatcggtc cgacgactat ggtaagagaa cctcgaggga
    14761 tttcgacatc gagattgttg aggaccatct tatcaggctt ccaaccaaag ctaccgtcct
    14821 ttattgcgac tgcgggagat ttgtcaagtt gatcaaggga tggcttctcc ggatcccttc
    14881 ttgaccctgc gaggaagata cggtaatcat cacgtccttc accctgtaag aatttctgga
    14941 tacggcctag gcaagcgact gctgcagcca tttgaggtaa tgtctctaat aaattttgaa
    15001 ggggagttga catgagtaag aggtaagata gtgaagtgaa cagttttgta gcatctaggc
    15061 ttcgtcgagc caccccgaag gtaatcggag gagacaggag tagtggtatg taggcaaaga
    15121 ctgagcatgt taacatcagc aatcggaagt tcgaccctgc ttgtaactcg tctacccgga
    15181 gcttttcgac gaagcgagag atgggactgg tgaggccgga tatctttata tttttcatgt
    15241 tgccgatgac actggaggta agcccgacac gcttttggat cccagccatc cagttccttt
    15301 gagagtcgcc catgaatcgc ataaagaatg atacgccgcc gacgcatatg caaacgacca
    15361 caattggcgc tataaaggcg acaccgagta ggttgtataa caaccaagca gaaagggcaa
    15421 cctcgatgac atttccccac tgaaataatt atgagttagc aatgctcatt tatccacgaa
    15481 cataaccctt cagagtcatg ttttcggaaa gtgaaatcga agttgaagcc agcatttacg
    15541 acgtgaggtc aacaacagct aatagatagg tgatgggaat agaccattag gtgtaaatat
    15601 gaaatcacgt acaagttcat gcatatacaa ggatcccttc ataatacgtt ctatgtcaat
    15661 gctcatcaag gtcaacgacg cattctcgtc ttcatccgcg gcacgggcct ctgtggtctt
    15721 cgtatagatg gccgttgcta gacacgcacg catcatgtat agcaatcgct gttggaaata
    15781 ccaatatagt gattgtgaaa tggcaattac agaatagatg cagatgctag cgccgataaa
    15841 gccatatccg atattggctg gaacaggctc agattgagat aagcgactag tgagactgct
    15901 tatgaatagg ggctggcaga aaaaggaccc aatcgccgcg agcttcgcgg gaatcggaag
    15961 tatcataggg acatagagcg ttttggccaa ggcttccatg agtccgattt catggccctt
    16021 acgtttggct tcatttatgt gtcgtgagaa gcgttcagaa agatgtgtgg cagcgaggtt
    16081 ttggtcaaga ggatacaggt cgcgtattcc taaggtctta tgatatccat caaaaaagag
    16141 cttgttaagc caggagaaaa cgcctaaagt atagataccg ctggtttctt ctgggctatg
    16201 gtctttcgag tcccaggcga cccatttggt tttccgctgg gcctcaagca agagcatggc
    16261 aatcttcata gctaacgcag ccgtaaagat agcggtaaat gcaatctcgg gccgggttga
    16321 ggaagccaac caataggttc gggcttgggc gatgtcaaat agaagtgtaa agaagaggta
    16381 ggcactaaga aagatggaag gccttggtga cttgctgtga tcgaagtaag agaggccgac
    16441 catgcaaaga gcagcagcaa ggcgaagggc ggatgagata atgtcaatac cactggcgcc
    16501 aaagggaagc accgtgatga ggataagttg tgagagttca agagacgcat atgagacgag
    16561 aacaccctag gtcgataagc attaacacga acgcgtacca attgactgaa ttcaacactc
    16621 acaagttttg ctagccggag caatggcgcg cccacgatcg tcggctttcg cacgagatgg
    16681 gcaacccgcc atggcgacgc aaggatgaaa atggctgcag gtgtgagtga gaagcacaac
    16741 tgttcgaact tgacagtgaa gtcgaagtct cctcgacaac ctaggacatt gggtcccaga
    16801 gattggtcat tagtatctgc gcacgcagaa aacaacatct tgctccaaac ctgttcaccc
    16861 aaacaggcct gtaataatat tgaatagata taagtttgct gacagccgca agagtcgcgg
    16921 atgaagcatc atggaagtct tatggtagtc agggtggcct atgtaagtag gtagccgaaa
    16981 acaaaggatc tctggtgagt ctactccgta ttaccaaccc caaagggcac aattccgaac
    17041 accgcatcgc gtatatcgac cccggtgccg ttatatcgtt aaggaaaatc aggggtaggt
    17101 aagcatcgtg taggtatcgt aaatcaccaa gtattgaatg agaaggggtg ctattagcgc
    17161 gatatcagca gtagctgata ggaattcgac atcacaagcg atgcgtcccc gcactttgcc
    17221 ctgtattcat aggcccatgg taggtgtcag catggaagcc agagaatatc taacatcagc
    17281 tcgtagggct ggactaatgc agtggagata tacgatcagt gcttatagta ataggccgag
    17341 attcgagtcg tactttttat aaccttgaaa ccttagtcca aggtcctttg gaaccgaggg
    17401 caggcagact tccagttacg ctagcgtttc tgatcctgga tatagcgtac ctacactccg
    17461 actagcaggt gcg
//
