LOCUS       BGC0002277              3145 bp    DNA     linear   PLN 15-JUL-2012
DEFINITION  Suillus grevillei isolate DSM 8404 clone 7.2.7 genomic sequence.
ACCESSION   BGC0002277
VERSION     BGC0002277
KEYWORDS    .
SOURCE      Suillus grevillei
  ORGANISM  Suillus grevillei
            Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina;
            Agaricomycetes; Agaricomycetidae; Boletales; Suillineae; Suillaceae;
            Suillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:14:43
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3145
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(<1..>3145)
                     /gene="greA"
     mRNA            complement(join(<1..206,267..1021,1078..1911,1961..2199,
                     2251..2387,2440..>3145))
                     /gene="greA"
                     /product="atromentin synthetase"
     protocluster    1..3145
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:3145]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..3145
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..3145
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..3145
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002277"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..3145
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             complement(join(1..206,267..1021,1078..1911,1961..2199,
                     2251..2387,2440..3145))
                     /NRPS_PKS="Domain: AMP-binding (61-465). E-value: 1.1e-65.
                     Score: 213.6. Matches aSDomain:
                     nrpspksdomains_greA_AMP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (604-671). E-value: 2.5e-11.
                     Score: 35.8. Matches aSDomain:
                     nrpspksdomains_greA_PP-binding.1"
                     /NRPS_PKS="Domain: Thioesterase (697-946). E-value:
                     1.5e-33. Score: 109.0. Matches aSDomain:
                     nrpspksdomains_greA_Thioesterase.1"
                     /codon_start=1
                     /gene="greA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     224.4; E-value: 3.6e-68)"
                     /gene_kind="biosynthetic"
                     /product="atromentin synthetase"
                     /protein_id="AFB76152.1"
                     /sec_met_domain="AMP-binding (E-value: 2e-75, bitscore:
                     240.0, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.5e-13, bitscore:
                     35.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASIAVTTTTTTTTAEFVTSPRTSIVPEQPNTLHDVIAQAVDSYP
                     LHELGFITSSAHDSSIQTKTFSAFNQYVRNLARAMLEWGKPTGSVVVVYLTEHEDNMTA
                     VWACLLAGFVPCLQPALSAQQAHKEGHVAHIKNLFGSATWLTSELGAEQINSISGLEVH
                     LLSELKSSAEKFTVAADWVAYEAKPDDEAILFLTSGSTGFSKAVVHTHRTILAACRAKG
                     QSYGLTSESQVLNWVGFDHVAGSLEMHITPLLYGASQLHVHASAILADPLRLLRLIDEK
                     SIELAFAPNFLLSKLTRDLEKRTDLFGSFDLSSIKRINSGGEAVVSKTAQAFAATMKQL
                     SKNPSAVSFVISAGFGMTETCAGCIYDPVDVLKNKPAHEFLDLGRPINGCEMRIVDPED
                     GATLRPDGESGELQVRGPMVFVRYYNNAEATSSSFVEGGWYRTGDVGIIENGVMRLSGR
                     IKDTVIVHGVSYGIPELETYLQTVEGVTHSFLAAAPYRAPGQETEGFIIFYSPTFDLNG
                     ADASTKLFATHRALRDICVKMITLPPQFVVPIPVNQMEKTTLGKLSRARLISLFKQGQL
                     AQHIARSEELLSEARGATFVAPSTETEKALAKIYAGIFNLAESEMSASDNFFELGGTSI
                     DVIRLKREGEAHFGLPEIPTIQILKHPVVSSLANYVNALLSKDSQTEEYDPIVPLQLTG
                     NKTPIFFVHPGVGEVLIFVNLAKYFQNERPFYALRARGFEPGHPFFTSMDEMVSCYAAA
                     VKRTQATGPYAIAGYSYGGVVAFEVAKRLEAMGDEVKFTGLINIPPHIADRMHEIDWTG
                     GMLNLSYFLGLVSKHDANDLAPALRPMTRNEQLEVVWKLSPPERLVELQLTPGKLDHWV
                     DIAGSLIECGKDYNPSGSVSAVDVFYAIPLRGSKADWLNNQLKPWSGFSRGDASYTDVP
                     GQHYTLMDFDHVPQFQKIFRGRLEARGL"
     aSDomain        complement(join(40..206,267..846))
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_greA_Thioesterase.1"
                     /evalue="1.50E-33"
                     /label="greA_Thioesterase.1"
                     /locus_tag="greA"
                     /protein_end="946"
                     /protein_start="697"
                     /score="109.0"
                     /tool="antismash"
                     /translation="PIFFVHPGVGEVLIFVNLAKYFQNERPFYALRARGFEPGHPFFTS
                     MDEMVSCYAAAVKRTQATGPYAIAGYSYGGVVAFEVAKRLEAMGDEVKFTGLINIPPHI
                     ADRMHEIDWTGGMLNLSYFLGLVSKHDANDLAPALRPMTRNEQLEVVWKLSPPERLVEL
                     QLTPGKLDHWVDIAGSLIECGKDYNPSGSVSAVDVFYAIPLRGSKADWLNNQLKPWSGF
                     SRGDASYTDVPGQHYTLMDFDHVPQFQ"
     aSModule        complement(40..2962)
                     /complete
                     /domains="nrpspksdomains_greA_AMP-binding.1"
                     /domains="nrpspksdomains_greA_PP-binding.1"
                     /domains="nrpspksdomains_greA_Thioesterase.1"
                     /final_module
                     /locus_tags="greA"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(592..666)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_greA_0003"
                     /evalue="1.10E-11"
                     /label="NRPS-te1"
                     /locus_tag="greA"
                     /protein_end="782"
                     /protein_start="757"
                     /score="37.1"
                     /tool="antismash"
                     /translation="QATGPYAIAGYSYGGVVAFEVAKRL"
     aSDomain        complement(join(925..1021,1078..1181))
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_greA_PP-binding.1"
                     /evalue="2.50E-11"
                     /label="greA_PP-binding.1"
                     /locus_tag="greA"
                     /protein_end="671"
                     /protein_start="604"
                     /score="35.8"
                     /tool="antismash"
                     /translation="ALAKIYAGIFNLAESEMSASDNFFELGGTSIDVIRLKREGEAHFG
                     LPEIPTIQILKHPVVSSLANYV"
     aSDomain        complement(join(1599..1911,1961..2199,2251..2387,
                     2440..2962))
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_greA_AMP-binding.1"
                     /evalue="1.10E-65"
                     /label="greA_AMP-binding.1"
                     /locus_tag="greA"
                     /protein_end="465"
                     /protein_start="61"
                     /score="213.6"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="QTKTFSAFNQYVRNLARAMLEWGKPTGSVVVVYLTEHEDNMTAVW
                     ACLLAGFVPCLQPALSAQQAHKEGHVAHIKNLFGSATWLTSELGAEQINSISGLEVHLL
                     SELKSSAEKFTVAADWVAYEAKPDDEAILFLTSGSTGFSKAVVHTHRTILAACRAKGQS
                     YGLTSESQVLNWVGFDHVAGSLEMHITPLLYGASQLHVHASAILADPLRLLRLIDEKSI
                     ELAFAPNFLLSKLTRDLEKRTDLFGSFDLSSIKRINSGGEAVVSKTAQAFAATMKQLSK
                     NPSAVSFVISAGFGMTETCAGCIYDPVDVLKNKPAHEFLDLGRPINGCEMRIVDPEDGA
                     TLRPDGESGELQVRGPMVFVRYYNNAEATSSSFVEGGWYRTGDVGIIENGVMRLSGRIK
                     DTVIV"
     CDS_motif       complement(1692..1781)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_greA_0002"
                     /evalue="1.20E-05"
                     /label="NRPS-A_a6"
                     /locus_tag="greA"
                     /protein_end="434"
                     /protein_start="404"
                     /score="17.7"
                     /tool="antismash"
                     /translation="PDGESGELQVRGPMVFVRYYNNAEATSSSF"
     CDS_motif       complement(2513..2572)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_greA_0001"
                     /evalue="2.40E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="greA"
                     /protein_end="211"
                     /protein_start="191"
                     /score="14.1"
                     /tool="antismash"
                     /translation="AILFLTSGSTGFSKAVVHTH"
ORIGIN
        1 ctacaagcca cgagcctcga gacgaccgcg gaagatcttt tggaattgcg ggacgtggtc
       61 aaagtccatg agcgtgtagt gttgcccagg cacatcagta tatgatgcat cgccacggct
      121 gaagccagac catggcttga gctggttgtt cagccagtca gccttgctgc cacggagggg
      181 aatagcatag aaaacgtcga ctgcggctga agagaggggg tgacatgtca acaagaggtg
      241 ccgaagtcag aaaacgaaga acttacaaac tgagcctgat gggttgtagt cctttccaca
      301 ctcaatcaga gacccggcaa tgtcaaccca gtggtcgagc ttcccgggag tcaactgcag
      361 ctcgaccaga cgttcagggg gagacaactt ccacaccacc tcgagctgct cattcctcgt
      421 cattggcctc agagcaggtg caagatcatt agcatcatgc ttcgacacga gaccgaggaa
      481 gtacgacaag ttgagcatgc cgcccgtcca gtcgatctcg tgcatgcggt ctgcgatatg
      541 aggaggaatg ttgatgagac cggtgaactt gacttcgtca cccatggcct ccagccgctt
      601 ggcaacttca aacgcgacaa caccgccgta actgtagccc gcgatggcgt atggtcccgt
      661 cgcctgagtc ctcttgactg ctgcggcata gcaagacacc atctcgtcca tagaggtaaa
      721 gaaggggtgt ccgggctcaa agccacgagc acggagtgcg tagaacggac gttcattttg
      781 gaagtatttg gcaaggttga cgaaaatgag aacctctccg acaccagggt gaacgaagaa
      841 gatgggagtc ttgttgccgg tgagttggag gggaacgatg gggtcgtact cctcagtctg
      901 gctatccttg gagagcaagg cgttcacata gttggcgagg ctggagacaa cggggtgctt
      961 gaggatttgg atggtaggga tctcagggag gccgaagtgt gcctctcctt cacgcttgag
     1021 tctggagatt atatatatta gtaaaggtcg ttgtacggcg gggacagata tattcacctg
     1081 atgacatcaa tggaagtccc gccgagttcg aagaagttgt cgctcgccga catctcgctc
     1141 tctgcgaggt tgaagatgcc cgcatatatc ttggcaagcg ccttttctgt ctcggtagac
     1201 ggggcaacga aggtagcgcc tcgcgcctcg ctgaggagct cctcagagcg ggcgatgtgc
     1261 tgagcaagct ggccttgctt gaagagactg atgagacgtg cacgagaaag cttgccgaga
     1321 gtggtcttct ccatctggtt cacagggatg gggacgacga actgaggtgg cagggtgatc
     1381 atcttcacgc aaatatcgcg cagggcgcgg tgcgtggcaa agagcttggt ggaggcgtct
     1441 gctccgttga ggtcgaacgt tggtgagtag aaaatgatga atccttcggt ctcctgtcca
     1501 ggagcgcggt acggagcagc ggcgaggaac gagtgtgtga ccccttcaac ggtctggagg
     1561 taggtctcga gctcagggat accatatgag acaccatgaa ctatgacagt gtctttgata
     1621 cgaccgctaa ggcgcatgac accattctcg atgatgccaa cgtctccggt gcggtaccag
     1681 ccgccctcaa caaagctaga ggacgtagcc tccgcgttgt tgtagtagcg tacgaagacc
     1741 attggtccgc gaacctgaag ctcgccgctc tccccgtcgg ggcgtagagt agcgccatct
     1801 tcggggtcaa cgatgcgcat ctcgcaacca ttgatgggac gaccgagatc aaggaactcg
     1861 tgagcaggct tgtttttgag gacatcgaca ggatcgtaaa tgcatccagc gctgcaacgg
     1921 cccgtcagct gaggtatcaa tattattaga gtatccttac catgtttctg tcattccaaa
     1981 cccagcggag atcacaaagg acaccgcaga ggggttcttg ctaagctgct tcatcgtagc
     2041 agcgaacgct tgtgctgtct tggaaaccac ggcttcaccc ccgctgttaa tgcgcttgat
     2101 ggaggaaagg tcgaagcttc cgaagaggtc ggtgcgcttc tctagatcac gggtgagctt
     2161 agagagcagg aagttcgggg cgaaagccag ttcaatcgac taaatataat gatgagagaa
     2221 acgttaggtt atgatggaat gtcaacatac cttttcgtcg atcagacgga gcagccggag
     2281 aggatcggcg aggatagcag atgcatgcac gtggagttgc gaagcaccgt atagcagagg
     2341 tgtgatgtgc atttccaatg atcccgcgac gtgatcaaat ccgacccctg aatgtcatga
     2401 ttaatttcaa aaagcttatt tagatgacgt gcgtcttaca gttcaagact tgagattcgg
     2461 aagtcaggcc gtaactttgc cccttggcgc ggcatgcagc aaggatcgta cggtgcgtgt
     2521 gcacaaccgc cttcgagaat ccagttgacc ctgaggtcag gaacaggatg gcctcgtcgt
     2581 ccggcttagc ctcgtatgca acccagtcag ctgcgacggt aaatttctcc gccgacgatt
     2641 ttaattcgga gagcaggtga acctccagac cagaaatgga gttgatctgt tcagccccaa
     2701 gctcgctggt cagccaagta gcagacccaa acaagttctt gatgtgagcg acatggccct
     2761 ccttgtgcgc ttgttgcgcg ctaagtgcgg gctgtaagca gggcacaaag ccagccaaca
     2821 gacacgccca aacagcagtc atattatctt cgtgctccgt caggtacacg acgacgactg
     2881 agcctgtggg tttgccccac tctagcatgg cacgtgcaag gttgcgaaca tattggttga
     2941 atgcactgaa ggtcttcgtc tgaatggagg agtcatgtgc cgaggaagtt atgaaaccaa
     3001 gctcgtgcaa cggatagctg tcaacagctt gagcgatgac gtcgtggaga gtattgggct
     3061 gttcagggac aatagatgtc ctgggggatg taacgaattc agcggtggtg gtggtggtgg
     3121 tggtggtgac tgcgatggat gccat
//
