BGC0002288: ashimide A biosynthetic gene cluster from Streptomyces sp. NA03103
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Location: 1,746,781 - 1,815,669 nt. (total: 68,889 nt).
This entry is originally from NCBI GenBank CP054920.1.

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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NRPS/PKS domains
General information about the BGC

MIBiG accession | BGC0002288
Short description | ashimide A biosynthetic gene cluster from Streptomyces sp. NA03103
Status | Quality: questionable The quality level of this entry. Status: active The status of this entry. Completeness: unknown Whether the entry covers everything needed for the pathway producing the compound(s)

Biosynthetic class(es)

- NRPS (Type I)

Loci
CP054920.1
1764340 - 1795669
via Heterologous expression, Enzymatic assays
Compounds

- ashimide A
- ashimide B

Species Streptomyces sp. NA03103 [taxonomy]
References

- [1] Comparative genome mining and heterologous expression of an orphan NRPS gene cluster direct the production of ashimides. Shi J et al., Chem Sci (2019) PMID:30996885
- [2]

Chemical products information
ashimide A Evidence: NMR [2]
Copy SMILES
C19H20N4O10
Chemical database entries
NPAtlas
ashimide B Evidence: NMR [2]
Copy SMILES
C19H19Cl1N4O9
Chemical database entries
NPAtlas
List of genes involved in compound(s) production

Identifiers | Position | Product | Functions | Evidence | Extra
--- | --- | --- | --- | --- | ---
HUT15_07560 QKW60381.1 | 1764358 - 1765065 (+) | response regulator transcription factor |  |  | copy AA seq copy Nt seq
HUT15_07565 QKW60382.1 | 1765062 - 1766510 (+) | HAMP domain-containing histidine kinase |  |  | copy AA seq copy Nt seq
HUT15_07570 QKW60383.1 | 1766781 - 1770011 (-) | amino acid adenylation domain-containing protein |  |  | copy AA seq copy Nt seq
HUT15_07575 QKW60384.1 | 1770008 - 1771360 (-) | serine hydroxymethyltransferase |  |  | copy AA seq copy Nt seq
HUT15_07580 QKW60385.1 | 1771514 - 1772980 (+) | MFS transporter |  |  | copy AA seq copy Nt seq
HUT15_07585 QKW60386.1 | 1773071 - 1773613 (+) | DUF1697 domain-containing protein |  |  | copy AA seq copy Nt seq
HUT15_07590 QKW60387.1 | 1773645 - 1774841 (-) | 3-deoxy-7-phosphoheptulonate synthase |  |  | copy AA seq copy Nt seq
HUT15_07595 QKW65597.1 | 1774851 - 1776710 (-) | chorismate-binding protein |  |  | copy AA seq copy Nt seq
HUT15_07600 QKW60388.1 | 1776833 - 1778209 (-) | cytochrome P450 |  |  | copy AA seq copy Nt seq
HUT15_07605 QKW60389.1 | 1778260 - 1778979 (-) | flavodoxin family protein |  |  | copy AA seq copy Nt seq
HUT15_07610 QKW60390.1 | 1778976 - 1780217 (-) | cytochrome P450 |  |  | copy AA seq copy Nt seq
HUT15_07615 QKW60391.1 | 1780387 - 1781208 (+) | SAM-dependent methyltransferase |  |  | copy AA seq copy Nt seq
HUT15_07620 QKW60392.1 | 1781435 - 1784704 (-) | amino acid adenylation domain-containing protein |  |  | copy AA seq copy Nt seq
HUT15_07625 QKW60393.1 | 1784701 - 1788696 (-) | amino acid adenylation domain-containing protein |  |  | copy AA seq copy Nt seq
HUT15_07630 QKW60394.1 | 1788824 - 1790008 (+) | NAD(P)/FAD-dependent oxidoreductase |  |  | copy AA seq copy Nt seq
HUT15_07635 QKW60395.1 | 1790034 - 1790702 (+) | class I SAM-dependent methyltransferase |  |  | copy AA seq copy Nt seq
HUT15_07640 QKW60396.1 | 1790829 - 1791605 (-) | winged helix-turn-helix domain-containing protein |  |  | copy AA seq copy Nt seq
HUT15_07645 QKW60397.1 | 1791867 - 1793252 (+) | phenylacetate--CoA ligase family protein |  |  | copy AA seq copy Nt seq
HUT15_07650 QKW60398.1 | 1793249 - 1795669 (+) | non-ribosomal peptide synthetase |  |  | copy AA seq copy Nt seq
HUT15_07655 QKW60399.1 msrB | 1795629 - 1796036 (-) | peptide-methionine (R)-S-oxide reductase MsrB |  |  | copy AA seq copy Nt seq

Biosynthesis information

#### Biosynthetic modules

Name
Unk01
Type
nrps-type1
Genes
HUT15_07650
Substrates
benzoxazolinate (evidence: Structure-based inference, ATP-PPi exchange assay)
Integrated Monomers
Domains
adenylation
Name
Unk02
Type
nrps-type1
Genes
HUT15_07570
Substrates
glycine, serine, alanine (evidence: Structure-based inference, ATP-PPi exchange assay)
Integrated Monomers
Domains
adenylation
Name
Unk03
Type
nrps-type1
Genes
HUT15_07620
Substrates
glycine (evidence: Structure-based inference, ATP-PPi exchange assay)
Integrated Monomers
Domains
adenylation
Name
Unk04
Type
nrps-type1
Genes
HUT15_07625
Substrates
alanine, 3-chloro-N-methylalanine, N-methylserine (evidence: Structure-based inference, ATP-PPi exchange assay)
Integrated Monomers
Domains
adenylation
Annotation changelog

#### Entry version: 2

Date
Changes
Submitters
Reviewers
2024-04-30
MIBiG v4 annotathon

- (ID: 5SRAIADCX5E626OGO7LYHFZD)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

#### Entry version: 1

Date
Changes
Submitters
Reviewers
2022-09-15
Entry added

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2022-09-15
Added NRP substrate specificities

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

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