LOCUS       BGC0002289              6464 bp    DNA     linear   BCT 17-JUN-2020
DEFINITION  Streptomyces leeuwenhoekii strain C34 (= DSM 42122 = NRRL B-24963)
            genome assembly, chromosome: chromosome.
ACCESSION   BGC0002289
VERSION     BGC0002289
KEYWORDS    .
SOURCE      Streptomyces leeuwenhoekii
  ORGANISM  Streptomyces leeuwenhoekii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 17:15:44
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 648369
            Orig. end    :: 654833
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     subregion       1..6464
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6464
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(4..1785)
                     /gene="sle_05770; ls2E"
     CDS             complement(4..1785)
                     /codon_start=1
                     /db_xref="GOA:A0A0F7VL39"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR011527"
                     /db_xref="InterPro:IPR027417"
                     /db_xref="InterPro:IPR036640"
                     /db_xref="InterPro:IPR039421"
                     /db_xref="UniProtKB/TrEMBL:A0A0F7VL39"
                     /function="Lasso-peptide 2 biosynthesis putative"
                     /gene="sle_05770__ls2E"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 205.8; E-value: 2e-62)"
                     /gene_kind="transport"
                     /product="Lactococcin transport/processing ATP-binding
                     protein LcnC-like"
                     /protein_id="CQR60039.1"
                     /transl_table=11
                     /translation="MKDSRWQLLRPVAARHRRELHSIAAWSLLGALPVLLSGRLVAHAV
                     DHGFLADDAVTGFLTLGGYGAALAVGSFAAWRAMAPMATVAEAVRDHLVRTTVSGGLRR
                     AVAGGVSSGGESVTALTSQTENARQILAALLMSAPSTGLTAAAALVGVVSLAPVSGLLA
                     LLPALTVGVVVVVRLSRSWRLRYSASLAADEHMTEQIAQRVQGLRDVVACGATRRARED
                     TDHAVDAQADAAAAVARASGARVGAVALGARLPLIALLVTAPWLSAQGALTPGELVGAV
                     TYLVSSLEPALRSLVQSVGNLALELGTVLARLTEYHREPKPGRVAESAPGTGTDGRCRR
                     RPAAPEPVPPDIELTLRAVTFRRGEGTDPPVLDRVCVTLPAGGHLAVVGPSGAGKSTLA
                     DLVCGLERPESGQVLLGGVPLPDLDPAWLRRSIALVPQEAYVFAGTVRDNLGYLAPEAD
                     DDRLDRAIEALDITALLAELGGYDGELRRPDLLSPAQRQLLTLARVYLSPARIVILDEA
                     TCHLGIAAEQRVERAFAQRGGTLVVIAHRMPSAARAQQVLVVDAGTVHSGTHRELLRTC
                     ATYADLVGHWGETGAPP"
     misc_feature    4..6461
                     /note="Lasso-peptide 2 gene cluster"
     gene            complement(1782..3542)
                     /gene="sle_05780; ls2E"
     CDS             complement(1782..3542)
                     /codon_start=1
                     /db_xref="GOA:A0A0F7VLY9"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR011527"
                     /db_xref="InterPro:IPR017871"
                     /db_xref="InterPro:IPR027417"
                     /db_xref="InterPro:IPR036640"
                     /db_xref="InterPro:IPR039421"
                     /db_xref="UniProtKB/TrEMBL:A0A0F7VLY9"
                     /function="Lasso-peptide 2 biosynthesis putative"
                     /gene="sle_05780__ls2E"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 285.3; E-value:
                     1.7e-86)"
                     /gene_kind="transport"
                     /product="Uncharacterized ABC transporter ATP-binding
                     protein YfiC"
                     /protein_id="CQR60040.1"
                     /transl_table=11
                     /translation="MRYTDRLLAQTVRAAGGHSAGLLAVAALDPLARLVLPAALAAAVD
                     AVVTGRTAWRQVLSACAVLAVSTACEAARETLQTTAEARGARALRRRLLHRVWDLGVTA
                     QARFDTGDVLSRVMESTAGAAAVTPVLVSTMSLLGTSVGGLVALFLIDVGLGLFFLAGV
                     LLTWWLLRWFVPLAGRVTLEYQEAQSDLSSRFVGALTGLRTIRASGTADRETARILTPL
                     SRLTAAGRGFWAAQRGAAWRIGLIMPVMQLGVLALAGHSLLHGRISHGEMLAAAGYLAL
                     ATGLMKQTAVFARLARARAGADRVGAVLRAGTAAGGRRPLTPGPGRLELRGVALCAKGR
                     PVLRGIDFTVPAGTEVALVGPPDSGASALARVAGGLVPADQGTVLLDDVPLHEVRPGEL
                     RDAIAYAFERPLLVGETLRDALTYTDRPPAAGRLAASLRAVHAEEFTARLPRGLETPVR
                     RLRLSGGEFQRLGLARAACRDARLVILDDALSSVDTVTEARVGTALGRLLADRTRLVVT
                     HRAATAARADLVAWLDAGTLRALAPHAVLLRDPAYRAIFRCGEEGDTASPSKPAHSSDG
                     PTAATVRTRT"
     gene            complement(3651..3782)
                     /gene="sle_05790; ls2A"
     CDS             complement(3651..3782)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:A0A0F7VSZ6"
                     /function="Lasso-peptide 2 biosynthesis"
                     /gene="sle_05790__ls2A"
                     /product="Hypothetical Protein sle_05790"
                     /protein_id="CQR60041.1"
                     /transl_table=11
                     /translation="MEHDEKTPYETPAVYGLGAFAEETGLYGVRNDEEINWHFDYWT"
     gene            complement(3811..4266)
                     /gene="sle_05791; ls2D"
     CDS             complement(3811..4266)
                     /codon_start=1
                     /db_xref="InterPro:IPR032708"
                     /db_xref="UniProtKB/TrEMBL:A0A0F7VRA3"
                     /function="Lasso-peptide 2 biosynthesis"
                     /gene="sle_05791__ls2D"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /note="hypothetical protein [Rhodococcus fascians] NCBI
                     Reference Sequence: WP_032380016.1"
                     /product="ls2D, homolog to larD; PFAM Transglut_core3
                     Transglutaminase-like superfamily Domain CL0125 alignment
                     3-111, HMM 3-113; HMM length 117; evalue 9.1e-24"
                     /protein_id="CQR60042.1"
                     /sec_met_domain="PF13471 (E-value: 2.9e-27, bitscore: 83.8,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLLVLVARVLAKRSPQCIRRVMTAFSAGTRAAGHGEARQARDEV
                     LSVSTRCCGPSACLPRSIAVALLCRSRGTWPTWCVGVVAAPPFRAHAWIEVAGEMVDEP
                     VDSACYRRFFAVEPAPARGFGHRGQGLGRSAAGGRPETRSARSESPR"
     gene            complement(4323..4580)
                     /gene="sle_05800; ls2C"
     CDS             complement(4323..4580)
                     /codon_start=1
                     /db_xref="InterPro:IPR008792"
                     /db_xref="UniProtKB/TrEMBL:A0A0F7VNU6"
                     /function="Lasso-peptide 2 biosynthesis"
                     /gene="sle_05800__ls2C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic-additional"
                     /product="Conserved Hypothetical Protein"
                     /protein_id="CQR60043.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1.3e-23,
                     bitscore: 71.7, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 3.6e-15, bitscore: 44.8,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPLMAEQVSVVETEYGMVLLDERTGEYWQLTPSASTIVARLRDG
                     GGVEEAVVALTRAFDVNEETARRDTLALVERLVQLQVMTR"
     gene            complement(4584..6461)
                     /gene="sle_05810; ls2B"
     CDS             complement(4584..6461)
                     /codon_start=1
                     /db_xref="GOA:A0A0F7VL41"
                     /db_xref="InterPro:IPR001962"
                     /db_xref="UniProtKB/TrEMBL:A0A0F7VL41"
                     /function="Lasso-peptide 2 biosynthesis"
                     /gene="sle_05810__ls2B"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 86.8; E-value: 2.2e-26)"
                     /gene_kind="biosynthetic"
                     /product="Asparagine Synthase"
                     /protein_id="CQR60044.1"
                     /sec_met_domain="Asn_synthase (E-value: 4.7e-31, bitscore:
                     97.3, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEPCVPRWFFVMLPDSDEAAALADRLPDTGTRRIPHASGRPWLVV
                     ALPGEQIVTGEAGADRVAVLGYSSATPAGLRTAAERAGSVEAFDALSHTLAGGFHLAAS
                     VGGRLRVQGSAAGVRRVFHTVLGGITVASDRADVLADLGGHRYDPTTLAIRLLRTLPHP
                     LGEQPLWQGVDHVPPESYLRIDADGRGAMMRRWWRRPEPHLTRRDGARLLRTALADAVA
                     VRTQAGGVVHCDISGGLDSTPITWFAAQGSARIVAATAYNADPGGTDDLRWARRALAVM
                     PSVQHRVLSLDKLPGFFEGVSEVDTRWDEPTAACLAAPRMRANIEDALSHTARMYLNGL
                     GGDHVLRGLPAWEHTLFRSRPWLAWQRARTNRMLSGESIATTARGLLDRRGYAQWLHAA
                     AASARREPSKDHRIGFDWDQPFAWPLWMTQDALRAVRGRIEELSATVEPLGQGRADHSE
                     LAMVRDGARVVRGTSQLGADAGLPFEAPLLDDRVVEACLAVRREERVSPLEIKPLMKEA
                     MRGLLPDDFLVRTTRAGGSPQSVRGYAAHHAELLDLCEASPLTDLGIIEIGALRRHNRP
                     QAGHRPDGHLVTTVNCALFLRNQVRGCAATPGVAHALPSESGPARALEE"
ORIGIN
        1 gggtcagggc ggcgccccgg tctcccccca gtggcccacc aggtcggcgt aggtggcgca
       61 agtccgcagc agttcccggt gcgtgccgct gtgcaccgtg cccgcatcca ccaccaggac
      121 ctgctgtgcc cgggcggctg agggcatccg gtgggcgatc accacgagcg tgccgccgcg
      181 ctgcgcgaag gcgcgctcca cacgctgctc ggccgcgatg ccgaggtggc aggtcgcctc
      241 gtcgaggatc acgatcctgg ccggggacag atagaccctg gccagggtca gcaactgccg
      301 ttgcgcgggg gagagcaggt cgggccgcct cagctcgccg tcgtatccgc ccagctccgc
      361 caggagcgcg gtgatgtcca gggcttcgat cgcccggtcg aggcggtcgt cgtccgcctc
      421 cggcgcgagg tatccgaggt tgtcccggac ggtgccggcg aagacatacg cttcctgagg
      481 taccagtgcg atgctgcggc gaagccacgc ggggtcgagg tccggcagcg gtacaccgcc
      541 caacagcact tggcccgact cgggacgctc cagcccgcac acgagatcgg ccagtgtcga
      601 cttgcccgcg ccgctcggtc cgacgaccgc caggtggccg cctgcgggaa gagtgacgca
      661 gacccggtcg agcacgggcg ggtcggtccc ctcaccccgg cggaaggtca ctgcacgcag
      721 ggtgagctcg atgtccgggg gcacgggctc gggggcggcg ggccggcgtc ggcaccgccc
      781 gtcagtgccg gtccccggcg cactctccgc cacccgcccg ggcttcggct cccggtgata
      841 ttcggtgagc ctggccagca ccgtgccgag ttccaaggcc aggttgccca ccgactggac
      901 cagggagcgc agtgcgggct cgagagagga gaccaggtag gtgaccgctc ctaccaactc
      961 gccgggtgta agggcaccct gcgccgacag ccagggcgcg gtcaccagca gggcgatcag
     1021 cgggaggcgg gcgccgagcg cgacggcgcc gaccctggcg ccgctcgccc gggcgaccgc
     1081 ggcggcggcg tcggcctgcg cgtccacggc gtggtcggtg tcttcccggg cccgcctggt
     1141 ggccccgcac gctacgacgt ctcgcaggcc ctggactctc tgcgcgatct gctcggtcat
     1201 gtgctcgtcg gcagccagtg acgccgagta gcgcaaccgc caggagcggg aaagccgcac
     1261 caccaccacc actccgaccg tgagcgcggg cagcagcgcg agcagtccgc tgacgggcgc
     1321 caggcttacc actccgacga gagccgccgc cgctgtcaga cccgtcgagg gcgctgacat
     1381 caacagcgcc gccaggattt gcctggcgtt ctccgtctgg ctggtgagcg ctgtgaccga
     1441 ctcgcctccg gaacttacgc ctccggccac cgcgcggcgc agcccgccgg ataccgttgt
     1501 ccgcaccagg tggtcgcgca ccgcctcggc cacagtcgcc atcggggcca tcgcgcgcca
     1561 tgcggcgaag gaacccactg cgagcgccgc gccgtacccg ccgagcgtca ggaacccggt
     1621 gaccgcgtcg tcggccagga agccgtggtc tacggcatgg gccaccaacc tgccggagag
     1681 cagcaccgga agagccccga gcagcgacca cgcggcgatg gagtgcagtt cccggcggtg
     1741 ccgggccgcc accgggcgca gtagctgcca tctggagtcc ttcatgtccg tgtcctgact
     1801 gtcgctgcgg tcggaccgtc ggagctgtgt gctggtttcg agggggaggc tgtgtcccct
     1861 tcctccccac accggaagat cgctcggtag gccgggtccc gcagcagcac agcgtgcggc
     1921 gccagcgcgc ggagggtgcc ggcgtccagc caggccacca gatcggcacg ggccgccgtg
     1981 gccgctctgt gggtgacgac cagccgggtg cggtcggcca gcaggcgccc aagcgcggta
     2041 ccgactcgcg cctcggtcac ggtgtccacg ctggagagcg cgtcgtcgag gatcaccaga
     2101 cgggcatcgc ggcaagccgc ccttgccaag cccaggcgct ggaactcgcc gccggacagt
     2161 cgcagccggc gcaccggcgt ctccagccct cggggcagcc gcgctgtgaa ctcctcggcg
     2221 tgcacggcgc gcaggctcgc cgccagacgc ccggcggcgg gtggccggtc ggtataggtg
     2281 agcgcgtcgc gcagggtctc gcccaccagc aacggccgtt cgaaggcata ggcgatcgcg
     2341 tcgcgcagtt ccccgggtcg cacctcgtgc agggggacat cgtcgagcag gacagtcccc
     2401 tgatcggcgg ggaccagtcc cccggccacc ctggcgagcg cggatgcgcc cgagtccggc
     2461 gggcctacga gggccacttc ggtgccggcg ggaacggtga agtcgatccc gcgcagcacg
     2521 ggccgtccct ttgcgcacag tgccacccca cgcagctcca gtcgccctgg tcctggtgtc
     2581 agcgggcgcc ggcccccggc cgcggttccc gctcggagca cggcgcccac gcggtcggcg
     2641 ccggcccgcg ccctggccag acgggcgaag accgcggtct gtttcatcag ccccgtggcg
     2701 agggcgagat acccggcagc agccagcatc tccccgtggc tgatccggcc gtgcaacaaa
     2761 ctgtgcccgg cgagcgcgag taccccgagc tgcatcaccg gcatgatcag gccgatccgc
     2821 caggccgcgc cgcgctgcgc ggcccagaat ccacgcccgg ctgcggtgag ccgggaaagc
     2881 ggcgtcagga tgcgggccgt ctcgcggtcg gccgttcctg aggcgcggat cgtccgcagt
     2941 ccggtcagcg cgccgacgaa ccgtgaggag aggtcgcttt gggcctcctg gtactcgagg
     3001 gtcacccggc ccgccagtgg gacgaaccaa cgcagcagcc accaggtcag cagcacgcct
     3061 gcgaggaaga acaggccgag cccgacgtcg atgagaaaga gcgcgaccag cccgcccacc
     3121 gaggtgccca acaaggacat cgtgctcacc agcacgggag tgacggccgc agcgccggcc
     3181 gtcgactcca tgactcggct gagcacgtcc ccggtgtcga acctcgcctg cgcggtgaca
     3241 ccgagatccc acacacggtg cagcaacctg cggcgcagtg cccgcgcgcc ccgagcctcg
     3301 gcggtcgtct gcaaagtctc acgggccgct tcgcaggctg tcgacaccgc gagcaccgcg
     3361 cacgccgaca ggacctgcct ccaagcggta cgccccgtga cgaccgcgtc cacggcagcg
     3421 gccagcgccg cgggcagcac caggcgggcc agcgggtcca aggcggccac cgcgagcagg
     3481 cccgcgctgt ggccgccggc tgcgcgcacg gtctgcgcca ggagccggtc ggtgtaccgc
     3541 actggtgagc ctcctcgtcg aagaacggcg tgaaccggtg cggtgcggcg gacgccgtgc
     3601 gtccgccgca ccgctgtggt cagaccctca tccgcgcaat gtccgcggca tcaggtccag
     3661 tagtcgaagt gccagttgat ctcctcgtcg ttgcgcaccc cgtacaggcc cgtctcctcg
     3721 gcgaacgcgc ccaatccgta caccgcaggg gtctcgtacg gcgtcttctc gtcgtgctcc
     3781 atggggttct ccttccgttg tcccgccgcg tcaacgcggc gactcactcc tggccgaccg
     3841 ggtctccggt cgacccccgg ccgctgaccg gccgagtcct tgtccgcggt gtccgaagcc
     3901 ccgggccgga gccggctcca ccgcgaaaaa cctccggtag caggcgctgt cgaccggctc
     3961 atcgaccatc tcgccggcca cctcgatcca ggcgtgcgcg cggaagggcg gggcggccac
     4021 cacccccacg caccaggtgg gccaggtccc gcgtgacctg cacagcagtg ccaccgcaat
     4081 cgacctgggc aggcatgccg agggcccgca gcaccgcgtg ctgacgctga gcacttcgtc
     4141 cctggcctgt cgcgcctccc catggccagc ggcgcgtgtg cccgcgctga acgccgtcat
     4201 cacacgccgg atgcactgcg gtgagcgctt ggccagcact cgcgccacca gcacgagcag
     4261 gcgcacccgg atccgcaccg cgagtcgtgg ccgcagtggc cggctgggga gtacaggagt
     4321 cgtcaccgtg tcatcacctg gagctgtacc agtctctcca ccagcgcgag cgtgtctctg
     4381 cgggcggtct cctcgttcac gtcgaaggcg cgggtgagcg ccacgaccgc ctcctcgaca
     4441 ccgcctccgt ctcgtagcct ggccacgatg gtcgatgcgc tcggtgtcaa ctgccagtat
     4501 tcaccggtac gttcgtcgag caggaccatc ccgtactcgg tctcgaccac ggagacctgc
     4561 tcggccatga gtggtgtcat gactcattcc tcaagggccc gggcgggccc ggactcggac
     4621 gggagcgcgt gtgcgacacc gggcgtcgca gcgcatccac gtacctggtt gcgaaggaac
     4681 agggcacagt tgacggtggt gaccaggtgg ccgtccggtc ggtgacccgc ctgcggtctg
     4741 ttgtgccggc gcagtgctcc gatctcgatg attccgaggt cggtgagcgg agaagcttcg
     4801 cacaggtcga gcagctccgc gtggtgggct gcgtatccgc gcacgctctg cgggctgccc
     4861 ccggcccgcg tcgtccggac caggaagtca tccggcagca ggccgcgcat cgcctctttc
     4921 atcaagggct tgatctccag cggagacact cgctcctcgc gcctgacggc caggcacgcc
     4981 tccactaccc ggtcgtcgag cagcggtgcc tcgaacggca ggcccgcgtc cgcacccagt
     5041 tgactcgtgc cgcgcaccac gcgggcgccg tcccgcacca tcgccagctc ggaatggtcc
     5101 gcacggccct ggcccaaggg ctcgaccgtg gcactcagct cctcgatcct gccgcgtacc
     5161 gcccgcagcg catcctgcgt catccacagc ggccaggcga acggctggtc ccagtcgaat
     5221 ccgatgcggt ggtccttcga cggctccctg cgtgccgagg cggcggcggc gtgcagccac
     5281 tgcgcgtagc cgcgccggtc aagcaagcca cgcgctgtcg tggcgatcga ttcgccgctc
     5341 agcatgcggt tggtccgggc tctctgccag gccagccagg gccgactacg aaagagggtg
     5401 tgctcccaag cgggcagccc gcgcagcaca tggtcgccgc ccaagccgtt cagatacatc
     5461 cgcgccgtat ggcttagggc gtcctcgatg ttcgcccgca tgcgcggcgc cgcgaggcag
     5521 gcggctgtcg gctcgtccca ccgggtgtcc acctcggaca ccccttcgaa gaagccgggg
     5581 agcttgtcga gcgacagcac gcggtgctgc accgacggca tcaccgccag agcccgcctc
     5641 gcccagcgca ggtcgtccgt gccgcctgga tccgcgttgt acgcggtcgc cgccacgatc
     5701 cgggcggagc cctgagccgc gaaccaggtg atcggggtgg agtccagacc gcccgagatg
     5761 tcgcagtgca ccacgccgcc cgcctgggta cggaccgcaa ccgcgtccgc caacgcggta
     5821 cgcagcagtc gggcgccgtc ccggcgcgtg aggtgcggct cgggacgccg ccaccatcgc
     5881 cgcatcatcg cgccgcggcc gtccgcgtcg atgcgcaggt acgactcggg cggcacgtgg
     5941 tccacgccct gccacaacgg ttgttcgccg agcgggtggg gcagggttcg cagcagccgg
     6001 atcgccagtg tggtcgggtc gtaccgatgc cctcccaggt cggccaggac gtcggcccgg
     6061 tcggaagcga cggtgatgcc gccgagaacg gtgtggaaga cccggcgcac cccggcggcg
     6121 ctgccctgga cgcgcagccg cccaccgacc gaggccgcca ggtggaaccc gcctgccagc
     6181 gtgtgagaaa gagcatcgaa cgcctcaacg ctgcccgcgc gttccgctgc cgtgcgcagc
     6241 ccggccggtg tggccgagga gtagccgaga acagcgaccc ggtcggcccc cgcttcaccc
     6301 gtgacgatct gttcaccagg cagtgcgacg accagccacg gcctgcccga ggcgtgcggg
     6361 atccggcgcg ttcccgtgtc gggcagccgg tccgcgagcg ccgccgcctc gtcgctgtcc
     6421 ggcagcatca cgaagaacca ccgcgggacg cagggttcca tgac
//
