LOCUS       BGC0002295              9315 bp    DNA     linear   BCT 16-DEC-2019
DEFINITION  Streptomyces hygroscopicus strain JCM 4712 minimycin biosynthetic
            gene cluster, complete sequence.
ACCESSION   BGC0002295
VERSION     BGC0002295
KEYWORDS    .
SOURCE      Streptomyces hygroscopicus
  ORGANISM  Streptomyces hygroscopicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:16:30
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9315
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1260
                     /codon_start=1
                     /gene="minT"
                     /gene_functions="transport (smcogs) SMCOG1106:major
                     facilitator transporter (Score: 101.9; E-value: 5.2e-31)"
                     /gene_kind="transport"
                     /product="MFS transporter"
                     /protein_id="QDX19368.1"
                     /transl_table=11
                     /translation="MICRVTKEELKNTVLRTGPAAYPDRSQRVRRLTLVALVIAALNLR
                     PGVTSLGPVLEEVRHSLAMSGTVAGLLTSIPAVCFALVGSTAPALARRYGASGAIAAGA
                     ALVTAGLVTRPFATDAALFVALTALSLAGIAIANVLLPAVVKHRFPDRVGAVTGLYSMA
                     LNVGASTAAAVTVPLTEAFGHDWRYGLGVWAILAAIAVPPWLAAARLQGRAAAGAADDG
                     GESQTHPRSRISRDPTAWALTAYFGLQASSAYVIIGWLPQMFRDAGLSAQSAGLLFSGT
                     SLLGVPLSFALSAAAGKLRSQSGIAAGIGLCGLAGFAGLWASASTTPWLWAFLLGVANC
                     SFPLALTMISMRGRDSATVVRLSGFVQSFGYLLSIPGPLVVGVLYGHTGGWRAPLAFIL
                     VMTLMQIGAGLLAGRNRQIG"
     protocluster    1..9315
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1564:5704]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1565..5704
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..9315
                     /SMILES="NC(CCC(=O)N)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..9315
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002295"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9315
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..1260
                     /gene="minT"
     misc_feature    43..45
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1565..5704
                     /gene="minA"
     CDS             1565..5704
                     /NRPS_PKS="Domain: AMP-binding (20-431). E-value: 1.1e-110.
                     Score: 361.9. Matches aSDomain:
                     nrpspksdomains_minA_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (943-1011). E-value: 1.4e-22. Score:
                     71.5. Matches aSDomain: nrpspksdomains_minA_PCP.1"
                     /NRPS_PKS="Domain: Thioesterase (1030-1156). E-value:
                     1.1e-27. Score: 89.8. Matches aSDomain:
                     nrpspksdomains_minA_Thioesterase.1"
                     /codon_start=1
                     /gene="minA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004:thioesterase (Score: 62.5; E-value: 5.5e-19)"
                     /gene_kind="biosynthetic"
                     /product="NRPS(A-Ox-T-TE-Tau)"
                     /protein_id="QDX19369.1"
                     /sec_met_domain="AMP-binding (E-value: 2.8e-119, bitscore:
                     386.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.2e-18, bitscore:
                     53.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPHVIPHTQRGDVCLTALLAEQASAAPHRTAVVHEDQSLTFAQL
                     LDHSRLLGARLRRAGVTRGSRVGVFMEPSLELMTNVWGILWAGGSYVPLSPEYPEERIA
                     YMMADAGVEVVLTQEFLRSRLQELSPAGVRTITSDEVFHSVESADEEISSAGLESCQAA
                     RPEDLAYVIYTSGSTGKPKGVMIEHRSIVSQMRWLTEACGIDGGRTILQKTPLSFDAAQ
                     WEILAPACGSTVVMGAPGIYRDPEAIVATIQRHGVTTLQCVPTLLQALLDTETLAGCGS
                     LRQVFSGGEALSRSLAAQFLDTMPDCSLVNLYGPTECTINASAFVVDRTAVEDGPRVMP
                     IGTPVSGTTFHVLDSAGREATVGEVGELHIGGIQVARGYLGRPDLTAERFVQDTFSSVP
                     GARLYRTGDLAHFNADGTVQFVGRTDNQVKLRGYRVELDEIRQAIETHDWVRSSAVLLR
                     DDEATGFQNLVAFVELNPKEAALMDQGNHGAHHQSKRSRLQVRAQLSHAGCRDAADLAG
                     RSVTALPGAEATPEQRARAFARKTYRFYEGGQVSREDILRLLAPPGRPGVAPRNPGSLS
                     RAELGGILRNFGQYLSDQRLLPKYAYASPGSLYATQLYLELDGIGGIGPGLYYYHPLHH
                     HLVLIGPTPRQSGPRASIHLLGKHSAIEPVYRNNIREVLEIEAGHMVGLFEEVLPDYGL
                     GIAAEYRPAVLHRLEGAEEDHYLGTIDLVPYDANESADELDIYVQAHAGRVDGLRAGQY
                     RYADGSLVRISDDLVLKKHVIAINQRVYERAGLGISLVATGPDSWRHYLDLGRKLQRLQ
                     MNGLNLGFMSSGYSSKSGNDLPAAKRLNRILTDCGLPTGPSYFFVGGRVSDEQLRGEDM
                     KEDVVHMQGPAELIKEDLAGLLPRYMLPNRIVVLDRLPLTANGKIDSKALEASQQADLA
                     LAARTFIAPRTRVERRVRDLWQTVLKQEQISVRDDFFELGGNSLLAVALVNRMNKAFEG
                     TVPLQVLFDAPTVEKLAARLDAEPSGPLTRLVPLQPEGTGTPIHCWPGLGGYPMNLRPL
                     AAALGKDRPVHGIQAHGINRGEDPYATVREMATADVEAIRSAQPEGPYILCGYSFGARV
                     AFEAARQLEQAGQRVEHLFLIAPGMPRLREEDTAGPTGRADFADRAFVALLHSVFAGTL
                     SGPQLDDCLRTVTDEDSFVDFVTSRFPGLGTELVQAVTGIVKRTYSPTYEFHELTGRRL
                     NAPVTLVKANDDNYSFIEHQDAFSVRPPSVHQLPSGHYELLREPHVTELAALVNDRLRT
                     AAGSPFPGQVSLSRVISQEAGVPHINIKHFPVALSEAQEKELLAALTSAVSNAFGCKED
                     VVSIAIEPVEQEAWHERVYEPEIVRRQDLLRKTPNY"
     aSDomain        1625..2857
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_minA_AMP-binding.1"
                     /evalue="1.10E-110"
                     /label="minA_AMP-binding.1"
                     /locus_tag="minA"
                     /protein_end="431"
                     /protein_start="20"
                     /score="361.9"
                     /specificity="substrate consensus: Gln"
                     /tool="antismash"
                     /translation="AEQASAAPHRTAVVHEDQSLTFAQLLDHSRLLGARLRRAGVTRGS
                     RVGVFMEPSLELMTNVWGILWAGGSYVPLSPEYPEERIAYMMADAGVEVVLTQEFLRSR
                     LQELSPAGVRTITSDEVFHSVESADEEISSAGLESCQAARPEDLAYVIYTSGSTGKPKG
                     VMIEHRSIVSQMRWLTEACGIDGGRTILQKTPLSFDAAQWEILAPACGSTVVMGAPGIY
                     RDPEAIVATIQRHGVTTLQCVPTLLQALLDTETLAGCGSLRQVFSGGEALSRSLAAQFL
                     DTMPDCSLVNLYGPTECTINASAFVVDRTAVEDGPRVMPIGTPVSGTTFHVLDSAGREA
                     TVGEVGELHIGGIQVARGYLGRPDLTAERFVQDTFSSVPGARLYRTGDLAHFNADGTVQ
                     FVGRTDNQVKLR"
     aSModule        1625..5032
                     /complete
                     /domains="nrpspksdomains_minA_AMP-binding.1"
                     /domains="nrpspksdomains_minA_PCP.1"
                     /domains="nrpspksdomains_minA_Thioesterase.1"
                     /final_module
                     /locus_tags="minA"
                     /monomer_pairings="Gln -> Gln"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     misc_feature    1715..1717
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS_motif       1808..1846
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_minA_0001"
                     /evalue="3.10E-03"
                     /label="NRPS-A_a2"
                     /locus_tag="minA"
                     /protein_end="94"
                     /protein_start="81"
                     /score="11.2"
                     /tool="antismash"
                     /translation="WGILWAGGSYVPL"
     CDS_motif       2069..2128
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_minA_0002"
                     /evalue="9.10E-12"
                     /label="NRPS-A_a3"
                     /locus_tag="minA"
                     /protein_end="188"
                     /protein_start="168"
                     /score="36.5"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGKPKGVMIEH"
     CDS_motif       2648..2734
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_minA_0003"
                     /evalue="8.10E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="minA"
                     /protein_end="390"
                     /protein_start="361"
                     /score="53.3"
                     /tool="antismash"
                     /translation="VGEVGELHIGGIQVARGYLGRPDLTAERF"
     CDS_motif       2822..2884
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_minA_0004"
                     /evalue="5.40E-10"
                     /label="NRPS-A_a8"
                     /locus_tag="minA"
                     /protein_end="440"
                     /protein_start="419"
                     /score="31.4"
                     /tool="antismash"
                     /translation="FVGRTDNQVKLRGYRVELDEI"
     aSDomain        4394..4597
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_minA_PCP.1"
                     /evalue="1.40E-22"
                     /label="minA_PCP.1"
                     /locus_tag="minA"
                     /protein_end="1011"
                     /protein_start="943"
                     /score="71.5"
                     /tool="antismash"
                     /translation="ERRVRDLWQTVLKQEQISVRDDFFELGGNSLLAVALVNRMNKAFE
                     GTVPLQVLFDAPTVEKLAARLDA"
     aSDomain        4655..5032
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_minA_Thioesterase.1"
                     /evalue="1.10E-27"
                     /label="minA_Thioesterase.1"
                     /locus_tag="minA"
                     /protein_end="1156"
                     /protein_start="1030"
                     /score="89.8"
                     /tool="antismash"
                     /translation="PIHCWPGLGGYPMNLRPLAAALGKDRPVHGIQAHGINRGEDPYAT
                     VREMATADVEAIRSAQPEGPYILCGYSFGARVAFEAARQLEQAGQRVEHLFLIAPGMPR
                     LREEDTAGPTGRADFADRAFVA"
     CDS_motif       4835..4909
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_minA_0005"
                     /evalue="1.20E-12"
                     /label="NRPS-te1"
                     /locus_tag="minA"
                     /protein_end="1115"
                     /protein_start="1090"
                     /score="40.1"
                     /tool="antismash"
                     /translation="QPEGPYILCGYSFGARVAFEAARQL"
     gene            5736..6689
                     /gene="minB"
     CDS             5736..6689
                     /codon_start=1
                     /gene="minB"
                     /product="Pseudouridine-5'-phosphate glycosidase"
                     /protein_id="QDX19370.1"
                     /transl_table=11
                     /translation="MTTRTPGPHSVPLVFTDEVAAALADGEPVVALESNVITHGLPYPD
                     NAATARKVEEAVRAGGAVPATIGIDDGRILIGMTDTDIERFASTPGIPKVSSRDLPAVL
                     ASGGTGASTVASSLVAAELAGITFFSSAGIGGVHRGAETTMDISSDLIQFTRSKVAVVC
                     AGAKKILDLRLTMEFLETHCVPVISYGFDDFPAFYCRSSGLRAPHRLDDETQIARAVDT
                     HWALGNPGGVLITSPTREEDAIDGDDIEEAIAAALRGAEADGVRGGAVTKYVMRAVDRA
                     TEGRSATANMAVLINTAEVGGRLAAAHARLRREETP"
     gene            6725..8566
                     /gene="minC"
     CDS             6725..8566
                     /codon_start=1
                     /gene="minC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115:HAD-superfamily hydrolase, subfamily IA, variant
                     (Score: 183.9; E-value: 5.2e-56)"
                     /gene_kind="biosynthetic-additional"
                     /product="HAD phosphatase and DUF4243 domain containing
                     protein"
                     /protein_id="QDX19371.1"
                     /transl_table=11
                     /translation="MTHVAMFDLDGTLVDSPRAIVETFAAAFEALGESPRDEADVRATI
                     GLPLEQAFAKLLGVPQDDPRVADGIARYQEAFRTVILPRARSLVFPGVAEGLAELRRHG
                     LTLTVATSKFHASADALLTAAGLRDHFAVLIGADDVTHPKPHPESGLRILQQLGARPEH
                     AVMVGDTTHDLKMAHAAGTRSVAVTYGVHGRAELASARPSYTAETFPEVVAYITGTLPD
                     SDVVDRLLNDRTYHIEFNGHLTNHVKHAVVALAGLGVAPERIKDYHDNYIRMTPYGCAV
                     EPARAPGRTIDDDNWLQLVGRRQDWAAYCEFFDRRERELGMPELLRRYLPHLLPGWAGA
                     LQHATIHLGWALAAHHRWMAIEGISYLAFSSVSCHPERSVPSGAHGAEEPKESLLRIAG
                     HWEANQPQLGAWVENLVGETSPEIHPELLRSGLQYRIARVLGEGHPLIHDTPGWIEGQD
                     PADSWDQLAYLVTLLYLAEPGDFLLLHLITALHAMRAISDALPAAQRPDVVKVYWTGIL
                     GVLFSRGHFSRPGKLTALDDLFDTATDDVSDPRWAREWDWHIARAVEEEEEHNPKLVHV
                     MRELWQRTGGRSIYRVAAGQFTATPELPPTFEQPPAD"
     gene            8593..9315
                     /gene="minD"
     CDS             8593..9315
                     /codon_start=1
                     /gene="minD"
                     /product="Uracil phosphoribosyltransferase"
                     /protein_id="QDX19372.1"
                     /transl_table=11
                     /translation="MTSALQQTTDVTAWTGPAPVASIEQYLGRNVHLLPQTNQLRALHT
                     VIRNREASRQDFVFHAGRIIRLLTEAALDLLPFEPYDVRTPVGSTYHGLRFTDGLLAVP
                     IVRAGESMESELRAVIPGIRIGKILIQRNKTTKRPHLYYQALPDDIADRHVLLLDPMLA
                     TGGTAVAAIELLLELGVPEEHIVFVNFITVPEGITAVCERFGRVRIVTSAIEERLNENA
                     YMVPGIGDFGDRYFGTDR"
ORIGIN
        1 atgatctgcc gtgtgacgaa ggaagagctc aagaacaccg tcttacgcac cggcccggcg
       61 gcgtaccccg accgctcaca gcgggtgcgc agactgacgc tcgtggcgct tgtgatcgcg
      121 gcgctgaacc tgcgcccggg cgtcaccagc cttgggcccg tcctcgaaga ggtccgtcac
      181 agccttgcca tgagcggcac ggtcgccggg ctgctgacgt ccatcccggc cgtgtgcttc
      241 gccctggtcg gctccacggc tcccgccctc gcccggcggt acggcgcgag cggcgcgatc
      301 gcggccggtg ccgcgttggt gacggccggg ctcgtgacac gtccgttcgc caccgacgcc
      361 gccctgttcg tcgcgctgac cgccctgtca ctggccggca tcgccatcgc gaacgtgctg
      421 ctcccggccg ttgtcaagca tcgcttcccc gaccgggtgg gcgcggtgac ggggctgtac
      481 tccatggcac tgaacgtcgg cgcctcgacc gctgccgccg tcaccgtgcc cctgaccgag
      541 gctttcggcc acgactggcg atacggcctg ggggtctggg ccatcctggc cgcgatcgcc
      601 gttccgccct ggctcgccgc cgcccgcctc cagggacggg cggcggcagg tgcggcggac
      661 gacggaggcg aatcgcagac acatccgcga agccggatct cccgcgaccc caccgcctgg
      721 gcgctcaccg cctacttcgg gctgcaggcg agttccgcct acgtcatcat cggctggctg
      781 ccacagatgt tccgcgatgc cggactgtcc gcacagagtg ccggcctgtt gttctccggc
      841 acctcgctcc tcggcgtacc gctgtccttc gccctgtcgg ccgcggccgg aaagctgcgc
      901 agccagagcg ggatcgcggc gggaatcggc ctgtgcggcc tggctggatt cgcggggctg
      961 tgggcctccg cctccaccac gccctggctg tgggcgttcc tgctcggcgt ggccaactgt
     1021 tccttcccac tggccctgac gatgatcagc atgcgcggcc gcgacagcgc caccgtcgtc
     1081 cggctctccg ggttcgtgca gagcttcggc tacctgctct cgattcccgg ccccctcgtc
     1141 gtcggcgtcc tctacggcca cacgggcggc tggcgtgcgc ccctggcctt catcctggtg
     1201 atgaccctga tgcagatcgg agccggtctg ctcgccggcc gcaaccgcca gatcggctga
     1261 gcagctctcg tcgccacagc gaatttcaca ccggctcgac ctccgtcaag ggaccgaaat
     1321 aattgttccc ttgtttcccg ccctgccatg cagagggcgt gaatttgctt cgccatgccc
     1381 ggaattaaca gccgcgctcg cgcggtgaat tcccgaagaa gtcggggtca atttccgcga
     1441 acgcgcacag aatttattga cctggccgga aaattcgtcc tagccttcca tcagcgatga
     1501 gctcagggca ccacaagtgc gcgagttccc gggaaatgcc cgctactgcg gaaaagagag
     1561 gtccatgagc ccccacgtga ttccccatac tcaacgtggt gacgtctgcc tgaccgcgct
     1621 gctcgccgaa caggcgagtg ccgcaccaca tcggaccgcc gtggtccacg aggaccagag
     1681 cctgacgttc gcccaactgc tggaccacag ccgcttactg ggagcccgcc tgaggcgcgc
     1741 cggagtgacc agaggcagcc gtgtcggcgt gttcatggaa ccctcgctgg agctgatgac
     1801 gaacgtctgg ggaatcctct gggccggcgg ttcgtatgtc ccgctctccc cggaataccc
     1861 cgaggagcgg atcgcgtaca tgatggcgga cgcgggggtg gaagtcgtcc ttacccagga
     1921 attcttgcgt tcccgcctcc aggaactgtc gccggccgga gtccgcacca tcacttcgga
     1981 tgaagttttc cattcggtgg agagtgccga cgaggaaatt tcgtcagcag gtttggaatc
     2041 ctgccaagcc gcccgtccag aagacctcgc gtacgtcatc tacacgtccg gcagcaccgg
     2101 aaaaccgaag ggggtgatga tcgagcaccg tagcatcgtc agccagatgc gctggctgac
     2161 ggaagcctgc ggaatcgacg gcggaaggac gatcctccag aagacccccc tgagtttcga
     2221 cgccgcccag tgggagatcc tcgcccccgc ctgcggcagc accgtggtga tgggtgcccc
     2281 cggaatctac cgggaccccg aggcgatcgt ggccaccatc caacggcacg gtgtgaccac
     2341 cctccagtgc gtccccacgc tcctgcaggc actcctcgac accgagaccc tcgccgggtg
     2401 cggctcgctg cgccaggtat tcagcggggg cgaggcgctg tcccgcagcc tcgccgctca
     2461 gttcctcgac accatgccgg actgctcgct cgtcaacctc tacggcccga ccgagtgcac
     2521 catcaacgcc tccgccttcg tcgtggaccg taccgcggtg gaggacggcc cccgggtcat
     2581 gccgatcggc acgccggtca gcggcaccac cttccacgtc ctggactcgg ccggacggga
     2641 ggcgacggtc ggcgaggtcg gcgaactgca catcggcggc atccaggtgg cccgcggcta
     2701 cctcggccgc cccgacctca cggccgagag gttcgtgcag gacaccttct cgtccgtccc
     2761 cggcgcccgc ctgtaccgca ccggcgacct cgcccacttc aacgccgacg gcacggtgca
     2821 gttcgtcggg cggacggaca accaggtcaa gctgcgcggc taccgggtcg agctggacga
     2881 gatacgccag gcgatcgaga cgcacgactg ggtccgcagc tcggccgtcc tgctcaggga
     2941 cgacgaggcc accggcttcc agaacctggt cgccttcgtg gagctgaacc ccaaggaagc
     3001 cgccctgatg gaccagggca accacggcgc ccaccaccag tccaagcgca gcaggctcca
     3061 ggtcagggcc cagctgtctc acgcgggctg ccgtgacgcc gcggacctgg ccggccgctc
     3121 ggtgaccgca ctccccggcg ccgaggccac acccgagcag cgggcccggg ccttcgcccg
     3181 caagacgtac cgcttctacg agggcggaca ggtgagccgg gaggacatcc tgcgcctgct
     3241 ggccccgccc ggccggccgg gcgtcgcccc gcgcaacccg gggagcctga gccgggccga
     3301 actcggcggg atcctgcgga acttcggcca gtacctcagc gaccagcggc tgctgcccaa
     3361 gtacgcctac gcctcgcccg gctctctcta cgccacccag ctgtacctcg aactcgacgg
     3421 catcggcggc atcggcccgg gcctgtacta ctaccacccg ctccaccacc acctggtgct
     3481 gatcggcccc accccccggc agtccggccc ccgggcgagt atccacctgc tcggcaagca
     3541 cagcgcgatc gagccggtct accgcaacaa catccgggag gtcctggaga tcgaggccgg
     3601 gcacatggtc ggcctgttcg aggaggtgct ccccgactac ggcctcggta tcgccgccga
     3661 gtaccggccc gcggtcctgc accgactcga gggcgccgag gaagaccact acttgggcac
     3721 gatcgatctc gtaccgtacg acgcgaacga gagcgccgac gagttggaca tctacgtcca
     3781 ggcgcacgcg ggccgggtcg acggcctgcg ggccgggcag taccggtacg ccgacggctc
     3841 cctggtgcgc atctcggacg acttggtgct caagaagcac gtcatcgcca tcaaccagcg
     3901 ggtctacgag cgggcgggcc tcggcatcag cctggtcgcc accggcccgg actcctggcg
     3961 gcactacctc gacctgggcc gcaagctgca gcgcctgcag atgaacggcc tgaacctcgg
     4021 cttcatgtcc tcgggctaca gctccaagag cggcaacgac ctgcccgcgg ccaagcggct
     4081 gaacaggatc ctcaccgact gcggactgcc cacgggaccg tcgtacttct tcgtcggcgg
     4141 gcgcgtcagc gacgagcagc tgcgcggcga ggacatgaag gaggacgtgg tccatatgca
     4201 gggaccggcc gagctgatca aggaagacct cgccggactg ctgccccgct acatgctgcc
     4261 caaccggatc gtcgtcctgg acaggctgcc cctgaccgcc aacgggaaga tcgacagcaa
     4321 ggccctggag gcctcgcagc aagccgacct cgcactggcc gcacggacgt tcatcgcccc
     4381 ccgcacccgc gtcgagcgtc gcgtccgcga tctctggcag accgtcctca agcaggagca
     4441 gatctctgtc agggacgact tcttcgagct gggcggcaac tccctgctcg ccgtggcgct
     4501 cgtcaaccgg atgaacaagg cgttcgaagg aacggttcct ctccaggtgc tgttcgacgc
     4561 ccctaccgtc gagaagctcg ccgcccggct ggacgccgag ccgtcagggc ccctcacccg
     4621 gctcgtcccg ctccagccgg agggcaccgg cacgccgatc cactgctggc ccggcctcgg
     4681 cggctacccc atgaacctgc gcccgctggc cgccgccctg ggcaaggacc ggcccgtcca
     4741 cggcatccag gcacacggca tcaaccgcgg cgaggacccc tacgccacgg tccgcgagat
     4801 ggcgacggcg gacgtcgagg ccatccgcag cgcacagccc gaaggcccct acatcctgtg
     4861 cggctattcg ttcggcgccc gggtcgcctt cgaggcggcc cgacagctgg agcaggccgg
     4921 ccagcgggtg gaacacctct tcctcatcgc cccgggcatg ccgcgcctgc gcgaggagga
     4981 cacggccggc ccgacgggcc gggcggactt cgccgaccgg gccttcgtcg ccctcctcca
     5041 ctccgtcttc gccgggaccc tcagcggccc gcaactggac gactgcctgc gcaccgtcac
     5101 cgacgaggac agcttcgtcg acttcgtcac cagccgcttt cccggcctcg gcacggaact
     5161 ggtccaggcc gtcaccggca tagtcaagcg cacctactcc ccgacgtacg agttccacga
     5221 gctgaccgga cgacgtctga acgcccccgt gaccctcgtc aaggccaacg acgacaacta
     5281 ctccttcatc gagcaccagg acgccttctc cgtccggccg ccgtctgtcc accaactgcc
     5341 gtccggccac tacgaactgc tgcgcgagcc gcacgtcacc gaactcgccg cgctggtcaa
     5401 cgaccggctg cgcaccgctg ccgggtcacc gtttccgggc caggtgtccc tgtcccgcgt
     5461 catctcccag gaggccggag tgccccacat caacatcaag cacttccccg tcgccctcag
     5521 cgaggcacag gagaaggaac tcctcgccgc actcacctcc gcggtgagca acgccttcgg
     5581 atgcaaggag gacgtcgtct ccatcgccat cgagccggtc gagcaggagg cctggcacga
     5641 gcgggtctac gagcccgaga tcgtccgccg ccaggacctg ctccgcaaga cgcccaacta
     5701 ctgaccgccg caccccggtt cctggaagga aacccatgac cacacgaaca cccggcccgc
     5761 attccgtccc cctggtcttc acggatgagg tggccgcggc cctcgccgac ggcgagccgg
     5821 tggtggccct ggagtcgaac gtcatcaccc acggactccc ttacccggac aacgcggcca
     5881 ccgcccgcaa ggtggaggaa gccgtaaggg ccggcggcgc ggtacccgcc accatcggca
     5941 tcgacgacgg ccggatcctg atcgggatga ccgacacgga catcgagcgc ttcgcctcga
     6001 cccccggcat ccccaaggtc agcagcaggg acctgccggc cgtcctcgct tccggcggca
     6061 cgggcgccag caccgtcgcc tcctcgctgg tggcggcgga actcgccggc atcaccttct
     6121 tctcctccgc cggcatcggc ggcgtccacc gcggcgccga gaccaccatg gacatctcct
     6181 cggatctgat tcagttcacc cgctccaagg tggcggtggt ctgcgccggc gcgaagaaga
     6241 tcctcgacct ccgcctgacc atggagttcc tggagaccca ctgcgttccc gtcatctcct
     6301 acggcttcga cgacttccca gccttctact gccgttccag cgggctgcgc gccccgcacc
     6361 ggctggacga cgaaactcag atcgccaggg ccgtggacac ccactgggcc ctgggcaacc
     6421 ccggcggtgt cctcatcacc agccccaccc gcgaggagga cgccatcgac ggggacgaca
     6481 tcgaggaagc catcgccgcg gcgctgcgcg gtgccgaggc cgacggggtg cggggcggcg
     6541 ccgtgacgaa gtacgtcatg cgcgccgtgg accgcgccac cgagggccgc tccgccacgg
     6601 ccaacatggc cgtcctcatc aacaccgccg aggtcggcgg ccggctcgcc gccgcccacg
     6661 cccggctccg acgcgaggag acgccgtgag caccgccagg gaaacggaga gggaagaggc
     6721 agctgtgaca cacgtggcga tgttcgacct ggacgggacg ctcgtcgact ccccgcgcgc
     6781 gatcgtcgag accttcgccg cggccttcga ggcgctcggc gagagccccc gggacgaggc
     6841 ggacgtccgg gccaccatcg gcctcccact ggagcaggcg ttcgccaagc tgctgggcgt
     6901 cccgcaggac gacccccgcg tcgccgacgg catcgcgcgc taccaggagg cgttccgcac
     6961 cgtcatcctg ccccgcgccc gctcgctggt cttccccggc gtcgccgagg gactggccga
     7021 actgcggcgc cacggcctga cgttgacggt ggccacgagc aagttccacg ccagcgccga
     7081 cgccctgctc accgcggccg gactgcgtga ccacttcgcc gtgctgatcg gcgcggacga
     7141 cgtcacccac cccaagccgc accccgagtc ggggctgcgc atcctccaac agctgggcgc
     7201 ccgccccgag cacgccgtga tggtcggtga caccacccac gacctgaaga tggcgcacgc
     7261 ggccgggacc agatcggtcg cggtcaccta cggagtgcac gggcgggcgg aactcgcgtc
     7321 ggcccggccg tcgtacacgg ccgagacctt ccccgaggtc gtggcgtaca tcaccggcac
     7381 gctgcccgac agcgacgtgg tcgaccggct gctgaacgac cgcacgtacc acatcgagtt
     7441 caacggtcac ctgaccaacc acgtcaaaca cgccgtcgtc gcgctcgccg gcctcggcgt
     7501 cgcgccggag cggatcaagg actatcacga caactacatc aggatgacgc cgtacggctg
     7561 cgcggtcgaa ccggccaggg cgcccgggcg gaccatcgac gacgacaact ggctccaact
     7621 cgtcggcagg cgccaggact gggccgccta ctgcgagttc ttcgaccggc gcgagcggga
     7681 gctcgggatg cccgaactgc tgcgccgcta cctgccgcac ctgctccccg gctgggccgg
     7741 agcgctccag cacgcgacca tccacctggg ctgggcactg gccgcccacc accggtggat
     7801 ggccatcgag ggcatctcct acctggcctt ctcctctgtg agttgccacc ccgagcgctc
     7861 agtcccctcg ggcgcacacg gcgcggaaga gccgaaggag tcgctgctgc gcatcgccgg
     7921 ccactgggag gcaaaccagc cgcagctcgg cgcatgggtg gagaacctgg tcggcgagac
     7981 ctcgcccgag atccacccgg agctgctcag gtccggactc cagtaccgga tcgcccgggt
     8041 gctcggcgag ggccacccgc tgatccacga cacccccggc tggatcgagg gacaagaccc
     8101 tgctgacagt tgggaccagc tcgcctacct ggtcaccctg ctgtatctgg cggaaccagg
     8161 ggacttcctg ctcctgcacc tcatcaccgc actgcacgcg atgcgggcca tctcggacgc
     8221 cctccccgcc gcccagcgcc ccgacgtggt caaggtctac tggaccggca tcctgggcgt
     8281 gctgttctcc cgcggacact tctcacgccc cggaaagctg accgcgctcg acgacctctt
     8341 cgacaccgcg acggacgacg tctccgaccc ccgctgggcc cgcgaatggg actggcacat
     8401 cgccagggcc gtcgaggagg aggaagagca caaccccaag ctcgtccacg tgatgcgcga
     8461 gctgtggcaa cgcacgggcg gcaggtcgat ctaccgggtc gccgcgggcc agttcaccgc
     8521 gaccccggag cttccgccca ccttcgaaca gccgccggcc gactgaccac cctccccacg
     8581 caaggaccac agatgaccag cgcactccag cagaccaccg acgtgaccgc gtggaccggc
     8641 cccgcgcccg tcgcctccat cgagcagtac ctcggccgca acgtccacct gctgccccag
     8701 acgaaccagc tgcgcgccct gcacaccgtc atccgcaacc gtgaagccag ccgtcaggac
     8761 ttcgtcttcc acgccggccg gatcatccgc ctcctgaccg aggccgccct cgacctcctg
     8821 cccttcgagc cgtacgacgt ccggactccc gtcggcagca cgtaccacgg gctgcgcttc
     8881 accgacgggc tgctcgcagt gcccatcgtg cgtgccgggg agagcatgga gagcgaactg
     8941 cgcgcggtga tcccggggat caggatcggc aaaatcctca ttcagcggaa caaaacgacc
     9001 aagcgaccgc acctgtacta ccaggcgctc cccgacgaca tcgccgaccg ccacgtcctc
     9061 ctgctcgacc cgatgctggc gaccggcggc acggcggtgg ccgccatcga gctgctgctc
     9121 gaactcggcg tgcccgagga gcacatcgtc ttcgtcaact tcatcaccgt gccggaaggc
     9181 atcacggcag tgtgcgagcg cttcggccgg gtgcggatcg tgacctccgc aatcgaggag
     9241 aggctcaacg agaacgccta catggtgccc ggcatcggcg acttcggaga ccgctacttc
     9301 ggaacggacc gatag
//
