BGC0002297 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002297 (Phormidium sp. LP904c)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	54,162	MC-LR/MC-LHty/MC-HphHty/MC-LHph/MC-HphHph	NRPS:Type I+PKS	100%

BGC0002297 - Region 1 - NRPS,T1PKS

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Location: 1 - 54,162 nt. (total: 54,162 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 54,163
Label		BGC0002297

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002297	1.88	NRP, Polyketide	MC-LR, MC-LHty, MC-HphHty, MC-LHph, MC-HphHph	Phormidium sp. LP904c
BGC0001017	0.92	NRP, Polyketide	microcystin LR	Microcystis aeruginosa PCC 7806
BGC0000426	0.83	NRP	sevadicin	Paenibacillus larvae
BGC0001133	0.83	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000465	0.81	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000979	0.72	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980	0.72	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0001023	0.70	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0002518	0.69	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0002437	0.68	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002297	1.00	NRP, Polyketide	MC-LR, MC-LHty, MC-HphHty, MC-LHph, MC-HphHph	Phormidium sp. LP904c
BGC0001017	0.78	NRP, Polyketide	microcystin LR	Microcystis aeruginosa PCC 7806
BGC0000426	0.75	NRP	sevadicin	Paenibacillus larvae
BGC0001133	0.74	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000465	0.72	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0002071	0.71	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0000979	0.69	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980	0.69	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0001023	0.69	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001128	0.68	NRP	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 54162:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (mal - X) + (X - Me-ohmal) + (NMe-Ser - D-Ala) + (Leu - X) + (Arg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mcyD

PKS_AT (2645..2929): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 188.6
inactive: 144.2
Methoxymalonyl-CoA: 65.9
Methylmalonyl-CoA: 64.4
Propionyl-CoA: 33.6
Isobutyryl-CoA: 24.6
Ethylmalonyl-CoA: 21.2
2-Methylbutyryl-CoA: 19.5
Benzoyl-CoA: 17.9
trans-1,2-CPDA: 15.9
CHC-CoA: 13.8
3-Methylbutyryl-CoA: 13.1
Acetyl-CoA: 12.3
fatty_acid: 8.7

mcyE

PKS_AT (561..840): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 162.7
inactive: 114.1
Methoxymalonyl-CoA: 56.0
Methylmalonyl-CoA: 46.6
Propionyl-CoA: 32.6
Isobutyryl-CoA: 29.8
Benzoyl-CoA: 18.6
Acetyl-CoA: 17.6
3-Methylbutyryl-CoA: 14.8
CHC-CoA: 11.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

AMP-binding (2449..2843): X

nrpys: D-Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHSGVVGK
Nearest Stachelhaus code(s):: D-Glu DPRHSGVVGK (100% 8Å match); Glu or D-Glu DPRHSGVVGK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

mcyG

AMP-binding (43..452): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: --LWVAASGK
Nearest Stachelhaus code(s):: Cin AILWVAASGK (79% 8Å match)
Stachelhaus code match:: 80% (moderate)

PKS_AT (1224..1517): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 169.1
inactive: 115.9
Methoxymalonyl-CoA: 70.6
Methylmalonyl-CoA: 66.7
Propionyl-CoA: 38.3
Ethylmalonyl-CoA: 34.4
Isobutyryl-CoA: 33.8
2-Methylbutyryl-CoA: 33.1
Acetyl-CoA: 29.1
CHC-CoA: 23.0
Benzoyl-CoA: 23.0
fatty_acid: 21.1
trans-1,2-CPDA: 19.3
3-Methylbutyryl-CoA: 14.2

mcyA

Search NORINE for peptide: strict or relaxed

AMP-binding (253..653): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLIDK
Nearest Stachelhaus code(s):: Ser DVWHISLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1736..2126): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mcyB

Search NORINE for peptide: strict or relaxed

AMP-binding (475..872): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DICVFGLVHK
Nearest Stachelhaus code(s):: Leu DICVFGLVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1526..1950): X

nrpys: D-bMe-Asp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DARHVGIFVK
Nearest Stachelhaus code(s):: D-bMe-Asp DARHVGIFVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mcyC

Search NORINE for peptide: strict or relaxed

AMP-binding (483..880): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DALFFGLVDK
Nearest Stachelhaus code(s):: Arg DALFFGLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)