LOCUS       BGC0002307             14987 bp    DNA     linear   BCT 16-FEB-2020
DEFINITION  Streptomyces humidus strain CA-100629 humidimycin biosynthetic gene
            cluster, complete sequence.
ACCESSION   BGC0002307
VERSION     BGC0002307
KEYWORDS    .
SOURCE      Streptomyces humidus
  ORGANISM  Streptomyces humidus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:18:03
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14987
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..14987
                     /country="Spain"
                     /db_xref="taxon:52259"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces humidus"
                     /strain="CA-100629"
     misc_feature    1..14987
                     /note="humidimycin biosynthetic gene cluster"
     protocluster    1..14987
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[4580:7092]"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4581..7092
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     cand_cluster    1..14987
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14987
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002307"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14987
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(83..775)
                     /gene="humR1"
     CDS             complement(83..775)
                     /codon_start=1
                     /gene="humR1"
                     /gene_functions="regulatory (smcogs) SMCOG1016:LuxR family
                     DNA-binding response regulator (Score: 119.4; E-value:
                     2.1e-36)"
                     /gene_kind="regulatory"
                     /product="response regulator"
                     /protein_id="QHZ32188.1"
                     /transl_table=11
                     /translation="MGINLFIISDSPVAIAGLEAIIDQDEKFRLVGRAVGNSDGIRCLD
                     KTELQPDVVILGEPSSKRTICESVQQIVSKYRREKSRPKIIVVSQQDDDDVIVAALRVG
                     VNGYIARIRSSEELLHSLQIVAKGGAAFSPTIASRLSGYFSMMQSLPELTGFSELTSRE
                     LEILELLARGLGNQQIARRLYLAEKTVRNYVSRIFAKLEVHDRATAAVVARDAGLGGHT
                     GVLAPQGI"
     misc_feature    complement(629..631)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1426..2529
                     /gene="humG"
     CDS             1426..2529
                     /codon_start=1
                     /gene="humG"
                     /gene_functions="regulatory (smcogs) SMCOG1048:sensor
                     histidine kinase (Score: 211.7; E-value: 3.2e-64)"
                     /gene_kind="regulatory"
                     /product="sensor histidine kinase"
                     /protein_id="QHZ32189.1"
                     /transl_table=11
                     /translation="MLDVPAALVFALLPQVAVLRPDPGGEVGYGTVWLLSLSTAIPLVA
                     RRLWPVPVFGFVLAAACVAPAAGVGPIPFLATAYALYTVATTRRQRPGQSAVFIACLCA
                     VGAVLLTASGGRGYQGGTRAVQVLFGLLVLGVTWAAGSAVRERRESTRRAIEHAAEQAK
                     VEERLRIARDIHDVVTHSVGLIAIKAGVANHVAATHPEEAREALKVIEDISRRALRDMR
                     ATLNALRREQDGGAHNLRPVLGLSDLPTLVRTAEAAGVRVDLRSLGAEEIPDGVALSAY
                     RMVQEALTNVVKHAAPTDCRVHLTAEQGTLTIDVTDDGPGPGHRPTVPGGGMGLVGMRE
                     RTAAHGGTLRTGPRPGGGFQVLATLPY"
     gene            2631..3533
                     /gene="humD1"
     CDS             2631..3533
                     /codon_start=1
                     /gene="humD1"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 168; E-value:
                     4.4e-51)"
                     /gene_kind="transport"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="QHZ32190.1"
                     /transl_table=11
                     /translation="MIDVQNLTKRYGQHTVVGGLTFAVRSGTVTGFLGPNGAGKSTTMR
                     MMLGLTRPDTGSATIDGRDYHELRYPARHVGALLEASAPHRGMTATDHLKWLAQSNRIP
                     RRRVAEVLDTVGLTEAAGRRIGTFSLGMGQRLGLAAALLGDPPVLVLDEPVNGLDAEGI
                     RWLRELLRSMAGEGRTVLVSSHLMTEMSMVADQLVVINRGQLLAETSVSDFIRRHGRTY
                     VRVRTEEPLRLNRELEQKGASLRLLPDGSLEVDGMPAAEISRLASVSGLPLEELSTHTA
                     SLEDTFLKLTGNGGSNTHV"
     gene            3526..4278
                     /gene="humD2"
     CDS             3526..4278
                     /codon_start=1
                     /gene="humD2"
                     /product="ABC transporter permease unit"
                     /protein_id="QHZ32191.1"
                     /transl_table=11
                     /translation="MSETVAAIRAEFTKLRGIRSTFVALLLFVPVSVLIAALGGWSAKG
                     AIESDSPSLRSDFTPEQAGLDGILYGQLALIVFGVLIVSSEYASGMMRVSLLAVPRRGR
                     LYTAKMTVTALAAAAIAIPVIVVSYLVTQIALGSHGASIDASGVPRALAGAVVYLMLMS
                     LFAAGVAAMARSAVLPLAVLLPMVLAGTQILSVIGATKEVARYFPDQAGMRMLTVDSHD
                     AYGGFAVLLVWTVAALAAGYLRHRRWDG"
     gene            4396..4524
                     /gene="humA"
     CDS             4396..4524
                     /codon_start=1
                     /gene="humA"
                     /gene_functions="biosynthetic-additional (lassopeptides)
                     predicted lassopeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="humidimycin precursor peptide"
                     /protein_id="QHZ32192.1"
                     /transl_table=11
                     /translation="MSAIYEPPALQEIGDFDELTKCLGIGSCDDFAGCGYAIVCFW"
     CDS_motif       4396..4458
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MSAIYEPPALQEIGDFDELTK"
                     /locus_tag="humA_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       4459..4524
                     /prepeptide="core"
                     /locus_tag="humA_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class I"
                     /score="12.50"
                     /molecular_weight="2186.5"
                     /monoisotopic_mass="2184.9"
                     /core_sequence="CLGIGSCDDFAGCGYAIVCFW"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MSAIYEPPALQEIGDFDELTK"
                     /leader_location="[4395:4458](+)"
     gene            4581..6404
                     /gene="humC"
     CDS             4581..6404
                     /codon_start=1
                     /gene="humC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 132.5; E-value: 3.1e-40)"
                     /gene_kind="biosynthetic"
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="QHZ32193.1"
                     /sec_met_domain="Asn_synthase (E-value: 2.7e-40, bitscore:
                     128.9, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFVVLPNCPAGERPAADLRAAQRIDHASGRPWIVGTWPEDEITV
                     VEAGPRRMVILGRTRLDLAATTATLGRMRSLHDVDAIASRLPGLFHLTVSMDGGTRVQG
                     SVSGVRQIFTAVTDGVTVAASAMDPLLRLTDADLDESVLAARLLAPGGAPWPLSLRTVR
                     RGIEPLGTGHWLELDADGRSRQKRWWTLPEPSLSLAQGADAVRSALSEAIATRVRPNRT
                     ISADLSGGLDSTSLCFLADRAGADLVTYHVMPLDHANEDTAWARKAAAMLPRARHHMLS
                     ADRAENVFDVGYTADHTNMAPEGPSTWASGLPHIEDLAKRATAEGSSLHLTGFGGDELF
                     GRMPACAWSLARAHPVSGLKLVNRYRLANRWSSGATLRALADRSTFAQNLTAVGARIGT
                     PPAPLAEPDFGWVFAPRMPTWATPDAVAAVRNLFTTAAARTPGPLDADRARHQALASIV
                     FEGKTVRQVNTVVAGTGIVWDAPFLDDRVLEAALSTEIDQRLASGRFKPLLTSAVRGVV
                     PSDILDRRDKGEFSAEAFRGIERNRARLLELCADSQLARLGLIDPAAFRSAVLNPGPMA
                     HHLHPIETTVACESWLRTHGRPRPQDPGERK"
     gene            6401..6664
                     /gene="humB1"
     CDS             6401..6664
                     /codon_start=1
                     /gene="humB1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic-additional"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="QHZ32194.1"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 5e-11, bitscore:
                     32.6, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 9.3e-27,
                     bitscore: 82.9, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.4e-14, bitscore: 44.1,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLSLARDVTLTSVDTGAVLLDGRRGRYWQLNQSGSAILRRLLDG
                     ESPAATAASLSAAAPVSEAQARQDVRALVESLSAANLVEVSQ"
     gene            6661..7092
                     /gene="humB2"
     CDS             6661..7092
                     /codon_start=1
                     /gene="humB2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="QHZ32195.1"
                     /sec_met_domain="PF13471 (E-value: 2.2e-28, bitscore: 88.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPAVAEQAPRLPPHRQIAPRCAAGAARLLVRLPPARLQRVLRQ
                     VSRGTRPATYGEVARARRSVVSVSTRCAGLGCLQRSVATLLLCRARGRWADWCTGFRIQ
                     PFGAHAWVEVGGRPVDEPGELSVFRTVLAVRCPDGRSRS"
     gene            7089..8054
                     /gene="humD3"
     CDS             7089..8054
                     /codon_start=1
                     /gene="humD3"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 204.6; E-value:
                     2.9e-62)"
                     /gene_kind="transport"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="QHZ32196.1"
                     /transl_table=11
                     /translation="MTAIRAEGLYAYYGTTPAVNGLDLTVPDGATFGFLGPNGAGKTTT
                     ISMLTTLLRPTAGRAEVAGYDVATRPAEVRRRIGIVFQESTLDMDLTAAENLRFQADLC
                     GLGRREARGAISAMLDMMELSERGDIPVQRFSTGLRRRLEIARGLLGAPRVLFLDEPTS
                     GLDAQTRAAVWEHLDRLRREQGITVFFTTHQLEEAEHCDRIAIIDRGKVITQGTPAELK
                     SVIGADLVLLRTEDDPSAADVLADRFGLTAELAPDGLRLRVSNGATLVPRLCTELGLAV
                     RSVTVTSPTLDDVFLHHTGSAIRESGTSARTLSNLGEGRR"
     gene            8051..8926
                     /gene="humD4"
     CDS             8051..8926
                     /codon_start=1
                     /gene="humD4"
                     /gene_functions="transport (smcogs) SMCOG1065:ABC-2 type
                     transporter (Score: 128.9; E-value: 3e-39)"
                     /gene_kind="transport"
                     /product="ABC transporter permease"
                     /protein_id="QHZ32197.1"
                     /transl_table=11
                     /translation="MSRIDTAPAVPGDKTATADRPPADRRGNAFRPYALLWRREMTRLR
                     HNPVRLAMGLVTPLLFLVVLGTGLDAASSTLGKAQLNDYRAYLFPGVLVMSVQAPAIAV
                     GISLVWDRRLGVLRQMLVSPFPRSSIVLGLALGGATTGGIYGLMVLSVGGIASIRYTPM
                     LLLVLLEMLLVALMFTAFGLLAAVTIKQVDTFQIVVNLSMMPLMFFSGAMFPPNGLPGW
                     LDAVVKLNPLTYGVDAVRRTLPGPDILTSEQTRLLLGGWNPPVLAELGLLAAITAVALG
                     LATYRFSRTP"
     gene            8947..9522
                     /gene="humE"
     CDS             8947..9522
                     /codon_start=1
                     /gene="humE"
                     /product="DoxX family membrane protein"
                     /protein_id="QHZ32198.1"
                     /transl_table=11
                     /translation="MNVKQAETTKAETTNAGLRKARFTKARFSKARLTRAWITNAWVGT
                     AARLLLAAVLAYAGLLKVQDLTEAGRTVALYRIVPDDSAQLVGGVLPFIELALALLLFA
                     GLATRAVAAGAATLMLMYIAAITSVWARGLSIDCGCFSAGGTLTSGAERGYAIDIARDL
                     VLLGAAVLLVRNPRTRYALDRWVLDVKE"
     gene            9528..10250
                     /gene="humF"
     CDS             9528..10250
                     /codon_start=1
                     /gene="humF"
                     /product="thioredoxin domain-containing protein"
                     /protein_id="QHZ32199.1"
                     /transl_table=11
                     /translation="MESTDVMVREMVRRRHRRRRTLLVSLVAAVVVAAAALVGAGLVRA
                     GDTEPGRAPDRVPARAADDKAGLVASTGGIRIDLYLDYLCPECRNTEKALAAELETLKT
                     DGDISVVYHPVTFLDGRSSPEGYSTRAASAAACAADRGRFEQYSAVLFDKQPPEGGPGL
                     SEAQLIDAGREAGITGGVFAKCVRDATYAPWVAYVSDVAASRKVALTPTVMVNGDRVEV
                     TGPDAAASLTRAVGEARE"
     gene            10247..11584
                     /gene="humH"
     CDS             10247..11584
                     /codon_start=1
                     /gene="humH"
                     /product="CapA family protein"
                     /protein_id="QHZ32200.1"
                     /transl_table=11
                     /translation="MTRLTVALSGDCMATRGALITSHPGAVELREVLRAADFAFTNLEV
                     VPAGGQGHPVHNAASGGCLIADPGVIDEVVGMGFTMVGCANNHALDLGVGGVLDTVNLL
                     DSKRIPFAGIGADLTAARRPVYVDRPGGSLALISCSSTFLPGHEAAAPSPDLPGRPGLN
                     PLRHHATLRVTPAQLDVLREIDGDTGLRARRAEARTLLGFDPAMPSPDRLTLFGTRFQA
                     ADTPGFTTTCDPKDLDEISRWVVEARRRADLVMVSVHSHEPGPTPGTPGEFLREFAHRV
                     IDEGADIVVGHGPHLLRGMELYRRKPIFYSLGNIVSQIELADRVSAEDYARVGVGEQRT
                     PGRYFAELSAHGRRFFAPHRRYWESLVPVLTFDEGTLVGVRLHPIDLGFGEPVHRRGRP
                     RTADPIAAKSILDDFARLAEPYGSTVAVGDTGTGELVFNTRQGQSS"
     misc_feature    10814..10816
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            11706..12380
                     /gene="humR2"
     CDS             11706..12380
                     /codon_start=1
                     /gene="humR2"
                     /gene_functions="regulatory (smcogs) SMCOG1016:LuxR family
                     DNA-binding response regulator (Score: 190.9; E-value:
                     2.8e-58)"
                     /gene_kind="regulatory"
                     /product="response regulator transcription factor"
                     /protein_id="QHZ32201.1"
                     /transl_table=11
                     /translation="MAEAVRVLIADDQALLRGSFRLLIDSTPGMKAIGEAGDGARAVEL
                     ARELKPDVVLMDLRMPVMDGIAATREICSDETLSGVRVLALTMFDMDEYVYPALQAGAS
                     GFMLKDVSPADLVAGIRVIATGEGVLAPTVTRRLIANFSRSDETTRATPRLVGLTKREQ
                     EVLVLIANGLSNAEISQQLVISLPTVKTHVSSLLAKVHARDRAQLVIIAYESGLAEPGM
                     PT"
ORIGIN
        1 gtcgatgctc tcctggatga cctgggcgat cgaggggacc ggcatcgaaa gggagggggc
       61 ggccgaaccg gtcgggcggg tctcagatgc cctgcggggc gagcacccct gtgtgcccgc
      121 ccaggccggc gtcgcgggcc accaccgccg ccgtggcccg atcgtgcacc tccagtttcg
      181 cgaagatccg cgagacgtag ttgcgcaccg tcttttcggc caggtagagc cgccgggcga
      241 tctgctggtt gcccagtcca cgggcgagca gttcgaggat ctccagttcc cggctggtca
      301 gctcggaaaa ccctgtcagt tccgggaggc tctgcatcat ggagaaatac ccgctgagcc
      361 tcgaagctat ggtcgggctg aaggcggcgc ctcccttggc cactatctga agggagtgaa
      421 gcagttcctc ggaggacctg atccgcgcga tatagccgtt cacgccgacc cgcagggcag
      481 cgacgatcac atcgtcgtcg tcctgttgtg acaccacgat tattttgggc cgtgacttct
      541 cacggcgata tttcgagacg atctgttgga cgctctcgca gattgttctc ttcgaggacg
      601 gctcgccgag tatgacgacg tccggctgta actcggtttt atcgagacat cgtatgccgt
      661 cggagtttcc caccgcgcgt ccgacgagtc tgaatttctc atcctgatcg atgatcgctt
      721 ccaggccggc gatggcaacc gggctgtcgc tgatgatgaa caggttgatt cccatggatt
      781 gacgtgaccc ccgtgtctgg tttccatgcc gaacaggaga aggatgacct tctcgcctgc
      841 gaattcatgg agtgagtgac cggcgcgtgc gtgcccaacg cgttggaaat gaatgagcga
      901 gcagtggccc gacaacgttc agaagttgca cgggtctcct tctggtcagc attcgttcga
      961 atgagagagg aagggtgcat tgcacactgg ctgctgccga gtatgcgtag gcaatcgaga
     1021 ggaacgcaag ttcacctcgt aagtggatgg acgcggttcc gtttggtgga tgactatcgg
     1081 ccagggggcc acgattcggc gagcggcccg gacgagaggg agttgccgga cctgtccgaa
     1141 acggggtaga ggcttgccgg cacctgcgat ttcgatcccg gggaggcgaa tcgacggcaa
     1201 gatcggaggc tgggtcactg gtgatcgagt gtctcgtgga cggcgtggat gtggccattt
     1261 tcgattcaca gggaattctt agcccgggca aaaatgctcg acctgtgact cacggccgca
     1321 aatcggaccg ccgccggagg ggccgtcgta cgaatgcgtt ggacgtcccg cgaaccgaac
     1381 caatacgttc acagcatggg tgatgtccct cgacggcgct tgatcatgct cgatgtgccg
     1441 gccgcactcg tgttcgcact gctcccgcag gtggccgtgc tgcggccgga ccccggcggc
     1501 gaggtgggct acggcaccgt gtggctgctg tcgttgagca ccgcgatccc gctggtcgcc
     1561 cggcggctgt ggcccgtgcc ggtcttcggg ttcgtcctgg ccgcggcctg cgtggcgccg
     1621 gccgcgggcg tcggaccgat cccgttcctc gccaccgcgt acgccctgta cacggtggcc
     1681 acgacgagga gacaacgacc aggacagtcc gcggtgttca tcgcctgtct gtgcgcggtg
     1741 ggcgcggtcc tgctcacggc gtcgggcgga cgcggctacc agggcgggac cagggccgta
     1801 caggtcctgt tcgggctgct cgtcctcggt gtcacctggg ccgcggggtc ggccgtcagg
     1861 gaacggcggg aaagcaccag gcgcgccatc gagcacgcgg ccgagcaggc gaaggtcgag
     1921 gaacgcctcc gtatcgcccg tgacatccac gacgtcgtca cgcacagcgt gggcctgatc
     1981 gcgatcaagg caggtgtcgc caaccatgtg gccgccactc acccggagga ggcacgggaa
     2041 gccctcaagg tgatcgagga catcagccgc agggcgttgc gcgacatgcg ggccaccctc
     2101 aacgcgctgc gccgggagca ggacggcggc gcccacaacc tccggccggt gctcgggctg
     2161 tcggacctgc ccactctcgt ccgcacggca gaggcggccg gtgtacgcgt cgacctgcgg
     2221 tccctgggcg cggaggagat cccggacggg gtcgcgctgt ccgcgtaccg gatggtccag
     2281 gaggcgctga ccaacgtcgt caagcatgcc gcgccgaccg actgccgggt gcacctcacc
     2341 gcggagcagg gcacgttgac gatcgacgtc accgacgacg gtcccgggcc ggggcatcgg
     2401 ccgaccgtgc ccggcggcgg aatgggcctc gtcgggatga gggaacggac cgccgcgcac
     2461 ggcggcaccc tccgtaccgg accgcggccg ggcggcggat tccaggtcct ggcgacgctg
     2521 ccctactgag ccggactcat ccgcaggtat gacttcggcc cgcgaaggcc gtccagcagt
     2581 cccgtcgcgg gaccgatgcg gacgccggga cgcccgacga gactcaccat gtgatcgacg
     2641 tccagaactt gaccaagcgg tacgggcagc acaccgtcgt gggcggcctc accttcgcgg
     2701 tgcggtcagg aaccgtcacc gggttcctgg ggcccaacgg agccgggaag tccacgacga
     2761 tgcggatgat gctcggcctc acccgcccgg acaccggatc cgcgacgatc gacggccgcg
     2821 actaccacga actcaggtac cccgcacggc atgtcggcgc cctgctggag gcgtccgcgc
     2881 cgcaccgcgg catgaccgcc accgaccatc tgaagtggct cgcgcagagc aaccggatcc
     2941 cccggcgacg ggtcgccgag gtcctggaca cggtcggcct caccgaggcg gccgggcggc
     3001 ggatcggaac gttctccctc ggtatgggcc aacggctcgg cctggccgcc gccctgctcg
     3061 gcgacccacc tgtcctcgtg ctcgacgagc cggtcaacgg ccttgacgcc gaaggcatcc
     3121 ggtggctgcg cgaactgctg cgctcgatgg ccggcgaagg ccggaccgta ctcgtctcca
     3181 gccatctgat gaccgagatg tccatggtcg cggaccagct ggtcgtcatc aaccgcggcc
     3241 aactcctcgc ggagaccagc gtgtcggact tcatccgacg ccacggacgg acctacgtga
     3301 gggtgcggac ggaggaaccc ctgcggctca acagggaact ggaacagaag ggcgcctcgc
     3361 tgcggctcct gccggacggc agcctggagg tcgacggcat gccggcggcg gagatcagcc
     3421 ggctggcctc ggtgagcggg ctcccgctcg aggagctgag cacccacacg gcatcccttg
     3481 aggacacctt cctcaagctc accggcaacg gaggaagcaa cacccatgtc tgagacggtc
     3541 gcggcaatcc gggctgagtt caccaagctg cgaggcatcc gcagcacgtt cgtcgcgctg
     3601 ctgctgttcg tccccgtcag cgtcctcatc gccgccctgg gcggctggtc cgcgaagggc
     3661 gcgatcgagt ccgacagccc ctcgctccgc tccgacttca cccccgaaca ggccggcctg
     3721 gacggcatcc tctacggcca gctcgccctg atcgtgttcg gagtgctcat cgtctccagc
     3781 gagtacgcgt cgggcatgat gcgcgtctcg ctgctcgccg tgccgcggcg aggacggctc
     3841 tacacggcga agatgaccgt caccgccctc gccgccgcgg cgatcgcgat ccccgtcatc
     3901 gtcgtcagct acctggtcac ccagatcgcc ctgggatccc acggcgcctc catcgacgcg
     3961 agcggcgtcc cccgcgccct ggccggcgcg gtggtctacc tgatgctgat gagcctgttc
     4021 gcggcaggcg tggccgcgat ggcccgaagc gccgtcctgc ccctggccgt actgctcccg
     4081 atggtgctcg ccgggaccca gatcctctcc gtcatcggcg cgaccaagga agtggcccgg
     4141 tacttccccg accaggccgg tatgcggatg ctcaccgtcg actcgcacga cgcgtacggc
     4201 ggcttcgcgg tgctgctcgt gtggaccgtg gcggcactgg ccgccggcta cctccggcac
     4261 cgccgatggg acggctgacg ggacgtgcat gaggcagctg tcccatgccg gtgggacatg
     4321 ccgccccgta atttcccaga caggtcacga gccgaacagg tcggcccgaa cgaccagacc
     4381 aactgggagg aacccatgtc cgcgatctac gagccccccg ccctccagga gatcggcgac
     4441 ttcgacgagc tcacgaagtg tctcggcatc ggaagctgcg acgacttcgc cggctgtggt
     4501 tacgccatcg tctgcttctg gtgatcacac cggtgccggt gcgcttgtgc gcaccggcac
     4561 cgcccggcga acgaggcgac atggaattcg tagttcttcc gaactgcccg gccggcgagc
     4621 gaccggccgc ggacctgcgg gcggcccagc ggatcgacca cgcgtcgggg cggccctgga
     4681 tcgtgggcac ctggcccgag gacgagatca cggtggtcga ggcggggcct cggcggatgg
     4741 tgatcctcgg ccgcacccgg ctggacctgg cggccacgac ggccacgctc ggccggatgc
     4801 gctcgctcca cgacgtggac gcgatcgcgt cccggctgcc gggactcttc cacctgacgg
     4861 tgtcgatgga cggcgggacc cgggtccagg gctccgtgtc cggcgtacga cagatcttca
     4921 ccgccgtgac cgacggggtg acggtggccg cgagcgctat ggacccgctg ctgcgcctca
     4981 ccgacgcgga cctcgacgag agcgtgctcg ccgcccgtct gctggcaccc ggcggagcac
     5041 cctggccgct ctctctgcgt accgtccgcc ggggcatcga gccgctcggc accgggcact
     5101 ggctcgaact cgacgccgac ggcaggtcac gacagaaacg ctggtggacg ttgccggagc
     5161 cgtccctgtc gctcgcgcag ggcgcggacg ccgtccgctc ggcgctgtcc gaggcgatcg
     5221 ccaccagggt gcgcccgaac agaacgatca gcgccgacct ctccggcggc ctggactcga
     5281 cctcactgtg cttcctcgcc gacagggccg gcgccgacct ggtcacgtac cacgtcatgc
     5341 cgctggacca cgccaacgag gacaccgcct gggcccgcaa ggccgccgcg atgctcccgc
     5401 gcgcccggca ccacatgctc tccgcggacc gcgccgagaa cgtgttcgac gtcggataca
     5461 ccgccgacca cacgaacatg gccccggaag ggccctcgac ctgggcctcc ggcctcccgc
     5521 acatcgagga cctggcgaaa cgggccacgg cggagggctc ctccctgcac ctgaccggct
     5581 tcggcggcga cgaactgttc ggccggatgc ccgcctgcgc ctggtccctg gcccgcgccc
     5641 acccggtcag cgggctgaaa ctggtgaacc gctatcggct ggccaaccgc tggtcgtccg
     5701 gcgcgaccct gcgagcactc gcggaccggt cgacgttcgc gcagaacctc accgccgtcg
     5761 gcgcccgcat cggcactccg cccgcgccgc tcgccgaacc cgacttcggc tgggtgttcg
     5821 cgccccgtat gcccacctgg gccacgccgg acgccgtggc cgccgtccgg aacctgttca
     5881 ccacggccgc cgcccggacc cccgggcccc tggacgcgga ccgggcccgc caccaggccc
     5941 tcgcctcgat cgtcttcgag gggaaaacgg tccgtcaggt caacacggtc gtcgcgggca
     6001 ccggcatcgt ctgggacgcg cccttcctcg acgaccgcgt actggaagcc gcgctgtcca
     6061 ccgagatcga ccagcgcctg gcgagcggac ggttcaagcc cctgctgacc tccgccgtgc
     6121 ggggcgtcgt gccctccgac atcctcgacc gccgtgacaa gggcgagttc agcgccgagg
     6181 cgttccgggg catcgaacgc aaccgggccc ggctgctgga actctgcgcg gactcgcagc
     6241 tcgcccggct cggtctgatc gacccggcgg cattcagatc cgcggtgctc aatcccgggc
     6301 cgatggccca tcacctccac ccgatcgaga ccaccgtggc gtgtgagagc tggcttcgga
     6361 cgcacggccg gccgcgcccc caggacccgg gagaacgcaa gtgaagctga gccttgcccg
     6421 cgacgtcacc ctgacgtccg tcgacaccgg cgccgtactc ctcgacggac gccgaggccg
     6481 ctactggcaa ctgaaccagt ccggatccgc catcctgcgc agactcctcg acggggagag
     6541 ccccgccgcg accgccgcga gcctgtccgc cgccgccccg gtgagcgaag cccaggcgcg
     6601 ccaggacgtg cgggccctcg tcgaatcgct gagcgcagcc aacctcgtgg aggtctcgca
     6661 gtgaccaccc ccgctgtcgc ggaacaggct ccgcgacttc caccgcaccg gcagatcgcg
     6721 cccagatgcg cggctggcgc ggcccgcctg ctggtccggc tgcccccggc ccggctgcaa
     6781 cgggtgctgc gccaggtcag caggggaacc cggcccgcca cgtacggcga ggtggcgcgg
     6841 gccagacggt cggtcgtctc ggtcagcaca cgttgcgcgg gcctcggctg cctccagcgc
     6901 tccgtggcca ccctcctgct gtgccgggcc cggggcaggt gggccgactg gtgcaccggg
     6961 ttccggatcc agcccttcgg cgcgcatgcc tgggtcgagg tgggcggacg accggtggac
     7021 gagccgggcg agctgagcgt gttcaggacc gtactggcgg tgcggtgtcc cgacgggagg
     7081 agccgctcat gacggcgatc cgggccgagg gcctctacgc ctactacggc accacaccgg
     7141 ccgtcaacgg actggacctg acggtgcccg acggcgcgac gttcggcttc ctcgggccga
     7201 acggcgccgg caagacaacc acgatcagca tgctgaccac gctgctcagg cccaccgcgg
     7261 gcagggcgga ggtggccggt tacgacgtgg cgacccgccc cgccgaggtc cgccgccgga
     7321 tcggcatcgt cttccaggag tcgacgctcg acatggatct cacagcggcc gagaacctcc
     7381 gcttccaggc cgacctgtgc ggactcggcc gccgtgaggc gcgaggcgcc atctccgcga
     7441 tgctcgacat gatggagctg tccgagcgcg gtgacatccc cgtacagcgg ttctccaccg
     7501 gccttcgccg ccggctggag atcgcccgcg gtctgctcgg cgccccgcgg gtcctcttcc
     7561 tcgacgagcc gaccagcggc ctcgacgcgc agacccgggc ggccgtctgg gagcacctgg
     7621 acagactccg gcgggaacag gggatcacgg tcttcttcac cacgcaccag ctggaggagg
     7681 ccgagcactg cgaccggatc gccatcatcg accggggcaa ggtgatcact caggggacgc
     7741 ccgccgagct gaagtccgtc atcggagccg atctcgtcct gctgcgcacg gaggacgacc
     7801 cgagcgccgc ggacgtcctg gcggaccgct tcggcctgac ggcggaactc gcccccgacg
     7861 gactacggct gcgcgtgtcg aacggcgcca ccctggtgcc gcggctctgc accgaactgg
     7921 gcctggccgt gcgctcggtg accgtcacct cgcccacgct cgacgacgtc ttcctgcacc
     7981 acaccggatc cgccatccgg gagagcggga cgagcgcgcg gacactcagc aacctcgggg
     8041 agggacggcg atgagccgga tcgacaccgc gccggccgta cccggcgaca agacggccac
     8101 ggccgaccgg ccgcccgccg accggcgggg aaacgccttc aggccgtacg cgctgctctg
     8161 gcggcgggag atgacacgcc tgcggcacaa cccggtccgc ctcgccatgg ggctggtgac
     8221 accactgctc ttcctcgtcg tcctgggcac cggccttgac gcggcgtcct ccaccctcgg
     8281 caaggcccaa ctgaacgact accgggccta cttgttcccc ggcgtgctgg tcatgtccgt
     8341 acaggccccg gcgatagcgg tcggcatctc cctcgtgtgg gaccgcaggc tcggcgtgct
     8401 ccgccagatg ctcgtctcgc cgttcccgag gtccagcatc gtgctcggcc tcgcactcgg
     8461 cggggccacc acgggcggga tctacggcct gatggtcctg tcggtcggag gcatcgcgag
     8521 catccggtat accccgatgc tcctgctcgt actgctcgaa atgctgctcg tcgcgctgat
     8581 gttcacggcg ttcgggctgc tcgccgcggt cacgatcaag caggtcgaca ccttccagat
     8641 cgtggtgaac ctcagcatga tgccgctgat gttcttctcc ggcgcgatgt tcccgccgaa
     8701 cgggctgccg ggctggctgg acgccgtggt caagctgaat ccgctcacgt acggcgtcga
     8761 cgcggtccgc cggaccctgc cgggccccga catcctgacc tcggagcaga cgcgtctgct
     8821 gctcgggggc tggaacccgc ccgtcctcgc ggaactgggg ctgctggccg ccatcaccgc
     8881 cgtcgcgctc gggctggcca cctaccgctt ctcccgtacg ccgtgagccg gacggagact
     8941 cgcgcgatga acgtcaagca ggcggagacc acgaaggcgg agaccacgaa cgcagggctc
     9001 agaaaggccc ggttcaccaa agcccggttc agcaaggccc gtctcaccag ggcctggatc
     9061 acgaacgcat gggtgggcac cgcggcacgg ctcctgctgg cggcggtcct ggcctacgcg
     9121 ggcctgctga aggtccagga cctcacggag gccggccgga cggtcgccct ctaccggatc
     9181 gtgcccgacg actccgccca actcgtcggc ggcgtcctgc cgttcatcga gctagcgctc
     9241 gcacttctgc tgttcgccgg actggccacc agggcggtgg cggccggcgc ggcgacgctg
     9301 atgctcatgt acatcgccgc catcacctcg gtgtgggcac ggggactgtc catcgactgc
     9361 ggctgcttca gcgccggcgg caccctgacg agcggggccg agcgcggcta cgccatcgac
     9421 atcgcacgcg acctggtcct cctcggtgcg gccgtcctcc tggtccgcaa tccgcgaacc
     9481 cggtacgcgc tggaccgctg ggtcctggac gtgaaggagt gacagacatg gagtcgaccg
     9541 acgtcatggt gcgggagatg gtgcgcaggc ggcaccggcg gcggcgcacc ctgctggtgt
     9601 ccctcgtggc cgcggtcgtg gtggccgccg ccgcgctggt cggcgccggc ctcgtccggg
     9661 cgggcgacac cgagcccggt cgggcaccgg accgcgtccc cgccagggcg gccgacgaca
     9721 aggcggggct ggtcgcctcg acgggcggca tacggatcga cctctacctc gactacctct
     9781 gccccgaatg ccgcaacacc gaaaaggccc tggccgccga actggagacc ctgaagacgg
     9841 acggagacat cagcgtcgtc taccacccgg tcaccttcct cgacggccgc agcagccccg
     9901 aggggtactc gacccgggcg gcgtccgcgg cggcctgtgc ggcggaccgg ggcaggttcg
     9961 agcagtactc cgcggtgctg ttcgacaagc agcccccgga gggagggccg gggctcagcg
    10021 aggcccagtt gatcgacgcg ggccgggagg cgggaatcac cggtggcgtc ttcgcgaaat
    10081 gcgtccggga cgccacgtac gccccgtggg tggcctacgt ctccgatgtc gccgcctccc
    10141 gcaaggtcgc gctgacaccg accgtcatgg tgaacggcga ccgtgtcgag gtcaccggac
    10201 cggacgcggc ggcctcactc acccgcgcgg tgggggaggc ccgggaatga cacggctgac
    10261 ggtggcgctc tccggtgact gcatggcgac cagaggggcg ctgatcacct cccaccccgg
    10321 cgccgtggaa ctccgcgagg tgctgcgcgc ggcggacttc gccttcacca acctggaggt
    10381 cgtccccgcg ggcggacaag gtcacccggt gcacaacgcg gcgagcggcg gctgcctgat
    10441 cgcggacccc ggggtgatcg acgaggtcgt cgggatgggc ttcacgatgg tcggctgcgc
    10501 gaacaaccac gcgctggatc tgggcgtggg cggtgtgctc gacacggtga acctgctgga
    10561 cagcaaacgg atccccttcg ccgggatcgg cgccgacctc accgccgccc ggcgaccggt
    10621 ctacgtcgac cggcccggcg gcagcctggc cctgatctcg tgcagctcga cgttcctgcc
    10681 cgggcacgag gcggcggctc cgtcaccgga cctgccgggc cggcccgggc tgaacccgct
    10741 gaggcaccac gcgacgctgc gggtgacgcc cgctcagctg gacgtgctcc gggagatcga
    10801 cggcgacacc gggttacggg cccgccgggc cgaggcgcgc acactcctcg ggttcgatcc
    10861 cgcgatgccc tcgcccgacc ggctcaccct gttcggcacc cgcttccagg ccgcggacac
    10921 cccgggattc accaccacgt gcgacccgaa ggacctcgac gagatctccc ggtgggtggt
    10981 cgaggcccgt cgccgcgcgg acctcgtcat ggtcagcgtg cactcccacg agcccggccc
    11041 gacccccggg acacccggcg agttccttcg cgagttcgcc caccgggtga tcgacgaggg
    11101 cgccgacatc gtggtcggac acggcccgca cctgctccgc ggcatggagc tctaccggcg
    11161 gaaaccgatc ttctacagcc tggggaacat cgtcagccag atcgaactcg ccgaccgggt
    11221 ctccgccgag gactacgcgc gggtcggcgt cggcgaacag cgcacaccgg gccggtactt
    11281 cgccgaactc agcgcccacg gccgccggtt cttcgccccg caccgccggt actgggagtc
    11341 actcgtaccg gtgctcacct tcgacgaggg cacgctcgtc ggtgtccgcc tccacccgat
    11401 cgacctgggc ttcggcgagc cggttcaccg ccggggccgc ccgcggacgg cggacccgat
    11461 cgccgccaag agcatcctcg acgacttcgc ccgcctcgcg gagccgtacg gatcgaccgt
    11521 cgcggtcggg gacacgggga cgggtgagtt ggtgttcaac acccggcaag ggcagagtag
    11581 ttgagacaaa atagtggggg accggggacg gtaggaaccg cgaagctcgg ccagcccgac
    11641 gaggggcatg cgacgagcgg cgcgccacga gcggtgtgcg aggaacggcg tgcgaggaac
    11701 acggaatggc tgaggcggtc cgggtactga tcgcggacga tcaggcgctg ttgcgcggga
    11761 gcttccgtct gctcatcgac tccacccccg gaatgaaggc gatcggcgag gcgggagacg
    11821 gcgccagggc ggtggaactc gcccgggaac tcaaaccgga cgtcgtcctc atggatctgc
    11881 gcatgcctgt catggacggc atcgcggcca cccgggagat ctgctccgac gagacgctgt
    11941 ccggtgtacg ggtcctggcc ctgacgatgt tcgacatgga cgagtacgtg tacccggccc
    12001 tgcaggccgg cgcgagcgga ttcatgctga aggacgtctc accggccgac ctggtcgccg
    12061 gcatccgcgt catcgccacc ggcgagggcg tcctggcgcc cacggtcacc cgccgcctca
    12121 tcgcgaactt ctcccgcagc gacgaaacga ctcgggcgac cccgcggctc gtcggactca
    12181 ccaagaggga acaggaggtg ctcgtgctga tcgccaacgg attgtcgaac gccgagatct
    12241 cccaacaact ggtgatcagt ctgccgacgg tgaagaccca tgtgagcagc ctcctcgcca
    12301 aggtgcatgc ccgtgatcgc gcccaactgg tcatcatcgc ctacgaaagc ggcctcgccg
    12361 agcccgggat gccgacctga ggcaccgcag cggaacaggg cgttcagcgc cggacgcgac
    12421 cggtgggcga cgcggtcccg cgcccgctgc cgtcctgcgc ggcggccttg gtgatgttgc
    12481 gggccatgcc accgaagaca accgagtgga agggggagac ggaccaccag tagacatgcc
    12541 ccagcagacc gcgcggatga aacagagcac gctgccgata acgagtacgg ccctgggcct
    12601 ccgcctcccc cctctccccc ctctcctccg tttccacgta catctccagc caggcgaggc
    12661 cgggcagccg catctcggcc cgcaaccgca gcagccgtcc gggctcgatc tcctcgaccc
    12721 gccagaagtc cagcgagtcg ccgacccgca ggcgttccgc gtcgcgacgc ccgcggcgca
    12781 gcccgacacc cccggcgaac ctgtccaacc agccccgcac cgcccaggcc agcgggaagg
    12841 agtaccagcc gttgtcaccc ccgatgccct cgatcacctt ccacagggcc tgccgggacg
    12901 cgtcgaccgg cagacaccgc tcgtcctggt agaggctgcc gcccgcccag tccgggtcgg
    12961 tgggcagggg gtcactgggc gcaccccgaa gagcggcgga cgaccaacgg gtggcgacct
    13021 gcgcctcccg caccttcctc agcgccaggg ccagcgcctc gtcgaagggc agcggccggc
    13081 ccggcaggtc gggaacgtac cgggcgatgt cgtgctcgtg gcagacgacc tcatggcgca
    13141 gcgactccgt gagcggccgg gccagggagg cgggcacggg ggtgacgagg ccgacccagt
    13201 ggctggacag cctcggggtc agcaccggaa ccggcacgat gagccgccga cgcagacctg
    13261 cgacgacggc gtagcggcac atcatctccc ggtaggtcag gacgtccggc ccgccgatgt
    13321 cgaacgcccg gttgacgtcg tccggcatgg cggccgagcc gacgaggtaa cgcagcacat
    13381 cgcgcacccc gatgggctgg gtgcgggtgt gcacccagct gggggtgacc atcaccggaa
    13441 gccgctcggt gaggtaccgc agcatctcga aggaggcgga ccccgagccg atgacgaccg
    13501 ccgcccgcag cacggtggcg ggcaccggcg cgtcgagaaa gatccgcccc acctcggccc
    13561 gcgaccgcag atgcggcgac agcgagtcct cggccacacc ccgaggcgtg agtccgccga
    13621 gatagacgat ccgccgtacg cccgcggcct gtgcctgctc ggcgaagacg cgcgcggcgc
    13681 gccggtcggt gtcctcgaac ccggaaccgg agccgagggc gtgcaccagg tagtacgcga
    13741 cgtcgacgcc gcgcatggcg gcggcgaccg actcggggtc cgtgacgtcc ccgcgaaccg
    13801 actcgacgtc gcccgcccag ggatggtcac ggagcttgtc cggcgaccgg gccagacacc
    13861 gcacccgatg ccccgcctcc agcaactccg gcaccaggcg tccgccgatg tagccggacg
    13921 ctccggtgac cagacagcga agcgtcgcgg ctccacggcc accatgcccc tcggcggcgt
    13981 ccggcccgtt ccactctccc catccaccca tgctcacgac cgtgcctcca cgcgttcgct
    14041 ccgacgctgc tgctgcctcg tcgtccgatc atcaccgcaa cggcccgacc gcgcccgtcg
    14101 ctggccatgg gaagcccagg tacccgaccg cactgccgcc gcccggtgcc gggtggtgcc
    14161 tgggaccagg gaactgcggc cacgtcgggt gcgacaggca ggagcccagg gcaggcacct
    14221 cccgttgtcg gtggcctccc gtacgctgcg cctcggatcg aacacttcgg attcggatag
    14281 agggtggggc gacgatgggc ggcgacactt ggagtcacac gggtccgtat caggctgatc
    14341 tcgcggccgc gttccggcag gctcagaagg acgagctggc caaggacaac cacggcttcg
    14401 agggtcggtc aatacaggag ttgtggctcg acccggagtg gcacgagtac gtctgcaccg
    14461 gcggtacggg cacggtgctg gacttccccc tcatggtgga cgccaccgac gccgacagcg
    14521 gcttcatgcg cccgctgacc gatcgggagg tacgtgcgtg gtctcctcag ggccggccca
    14581 ccttccaaga gtgggacgag gccctcgact ccgattgctt ggccttcccg gaccgcgccc
    14641 aggggaactg cacggtcctc tactgcgaag gccggcccac gcagatcggc tactggggtg
    14701 tcaccgccga ctagcacgac ccgcctctct tcgacgcggc acccgcctgc cacccacccg
    14761 cacacacgcg aagaccccgt cctcagctga agcgctgatg gcggggcctt taggcatctc
    14821 attttcaagg tgcccccggc aggattcgaa cctccaccgg gctccggagc acgtgggatt
    14881 cccatgtgtt tccgcaggtc ggcggcttac ctgacgggtt gttgggaccg tcgtcccgcg
    14941 catgtcccgc gaaataaaaa gctcgttcta tcgctttgcc tcgttcg
//
