BGC0002308 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002308 (Streptomyces sp)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	52,415	High	camporidine A/camporidine B	other:other+PKS

BGC0002308 - Region 1 - T1PKS

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Location: 1 - 52,415 nt. (total: 52,415 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 52,416
Label		BGC0002308

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

camA: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

camG: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

camH: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

camI: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

camJ: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

camL: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

camT: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Transferases: aminotransferase

camM: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Hydrolases: esterase

camB: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

camC: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

camD: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

camE: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

camR: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

camS: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002308.2	1.00	PKS, other (other)	camporidine A, camporidine B	Streptomyces sp.
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.68	PKS	oronofacic acid	Pseudomonas syringae
BGC0001254.3	0.66	PKS	ACT-Toxin II	Alternaria alternata
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002308.2	1.00	PKS, other (other)	camporidine A, camporidine B	Streptomyces sp.
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.68	PKS	oronofacic acid	Pseudomonas syringae
BGC0001254.3	0.66	PKS	ACT-Toxin II	Alternaria alternata
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 52415:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ccmal - ccmal) + (ccmal - ccmal) + (pk)
Non-colinear ordering:: camE + camD + camC

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

camC: pk

PKS_AT (567..861): pk

ATSignature: Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 207.9
Methylmalonyl-CoA: 195.8
Methoxymalonyl-CoA: 163.0
2-Methylbutyryl-CoA: 142.6
trans-1,2-CPDA: 107.3
Propionyl-CoA: 103.0
Isobutyryl-CoA: 89.5
Malonyl-CoA: 84.4
Benzoyl-CoA: 83.6
CHC-CoA: 79.6
Acetyl-CoA: 39.4
3-Methylbutyryl-CoA: 38.8
fatty_acid: 35.9
inactive: 25.6

camD: mal - mal

PKS_AT (561..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 197.6
inactive: 97.5
Methoxymalonyl-CoA: 56.8
Methylmalonyl-CoA: 49.7
Propionyl-CoA: 25.1
Isobutyryl-CoA: 23.0
Acetyl-CoA: 10.8
Benzoyl-CoA: 9.2
fatty_acid: 7.3
CHC-CoA: 6.9
Ethylmalonyl-CoA: 4.3
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -1.1

PKS_AT (2304..2596): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 185.8
inactive: 88.9
Methoxymalonyl-CoA: 56.7
Methylmalonyl-CoA: 50.9
Propionyl-CoA: 26.6
Isobutyryl-CoA: 23.1
fatty_acid: 11.7
Acetyl-CoA: 8.7
Benzoyl-CoA: 8.4
Ethylmalonyl-CoA: 7.3
CHC-CoA: 5.0
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.7

camE: mal - mal - mal

PKS_AT (489..782): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.2
inactive: 102.4
Methylmalonyl-CoA: 71.9
Methoxymalonyl-CoA: 69.9
Isobutyryl-CoA: 41.8
Ethylmalonyl-CoA: 41.4
Propionyl-CoA: 36.6
Benzoyl-CoA: 35.5
CHC-CoA: 28.0
trans-1,2-CPDA: 22.2
2-Methylbutyryl-CoA: 20.8
Acetyl-CoA: 20.2
fatty_acid: 12.7
3-Methylbutyryl-CoA: 4.3

PKS_AT (1516..1833): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 194.2
inactive: 79.2
Methoxymalonyl-CoA: 78.6
Methylmalonyl-CoA: 67.5
Isobutyryl-CoA: 52.1
Propionyl-CoA: 38.2
Ethylmalonyl-CoA: 27.4
fatty_acid: 25.7
Acetyl-CoA: 24.1
2-Methylbutyryl-CoA: 15.1
CHC-CoA: 10.4
Benzoyl-CoA: 9.8
trans-1,2-CPDA: 9.0
3-Methylbutyryl-CoA: 4.7

PKS_AT (3292..3588): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 210.0
inactive: 97.8
Methoxymalonyl-CoA: 63.0
Methylmalonyl-CoA: 49.3
Propionyl-CoA: 41.3
Isobutyryl-CoA: 27.3
Acetyl-CoA: 23.3
2-Methylbutyryl-CoA: 20.0
fatty_acid: 11.5
Ethylmalonyl-CoA: 11.1
Benzoyl-CoA: 9.3
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

camR: Acetyl-CoA

CAL_domain (45..498): Acetyl-CoA

minowa: Acetyl-CoA

Prediction, score:

Acetyl-CoA: 41.8
fatty_acid: 14.7
shikimic_acid: 0.0
NH2: -0.3
AHBA: -3.8

Link to PARAS substrate predictor