BGC0002344 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002344 (Nonomuraea coxensis DSM 45129)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T3PKS,NRPS	1	84,275	High	A50926 A/A50926 B	NRPS:Type I

BGC0002344 - Region 1 - NRPS,T3PKS

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Location: 1 - 84,275 nt. (total: 84,275 nt) Show pHMM detection rules used

Region on contig edge.

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 84,276
Label		BGC0002344

Tailoring functions

Oxidoreductases: oxygenase

Nocox_13375: Cyclization

MITE: MITE0000038Cyclization (identity: 70.10%)
smcogs: SMCOG1007: cytochrome P450

Nocox_13380: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Nocox_13385: Cyclization

MITE: MITE0000041Cyclization (identity: 79.40%)
smcogs: SMCOG1007: cytochrome P450

Nocox_13390: Cyclization

MITE: MITE0000027Cyclization (identity: 72.50%)
smcogs: SMCOG1007: cytochrome P450

Transferases: acyltransferase aminotransferase glycosyltransferase halogenase methyltransferase

Nocox_13270: aminotransferase

smcogs: SMCOG1019: aminotransferase

Nocox_13310: methyltransferase

smcogs: SMCOG1248: methyltransferase

Nocox_13340: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

Nocox_13395: flavin-dependent tyrosine-like hpg halogenase

halogenases: flavin-dependent tyrosine-like hpg halogenase
MITE: MITE0000028Halogenation (identity: 82.00%)
smcogs: SMCOG1119: halogenase

Nocox_13400: Glycosylation

MITE: MITE0000033Glycosylation (identity: 75.40%)
smcogs: SMCOG1102: glycosyltransferase

Hydrolases: Acylation

Nocox_13405: Acylation, Hydrolysis

MITE: MITE0000035Acylation, Hydrolysis (identity: 72.40%)

Lyases: hydratase

Nocox_13280: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Nocox_13285: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: Acylation ligase synthase

Nocox_13295: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

Nocox_13350: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Nocox_13355: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Nocox_13360: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Nocox_13405: Acylation, Hydrolysis

MITE: MITE0000035Acylation, Hydrolysis (identity: 72.40%)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002344.2	1.20	NRPS	A50926 A, A50926 B	Nonomuraea coxensis DSM 45129
BGC0000289.5	0.59	NRPS, saccharide	A40926	Nonomuraea gerenzanensis
BGC0000351.5	0.49	NRPS	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0002341.2	0.47	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.46	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0001249.5	0.42	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.42	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.42	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.39	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.39	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002344.2	0.98	NRPS	A50926 A, A50926 B	Nonomuraea coxensis DSM 45129
BGC0002341.2	0.77	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.76	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000351.5	0.72	NRPS	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0001128.4	0.71	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.71	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 84275:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpg - D-Tyr) + (Dhpg) + (D-Hpg - D-Hpg - Tyr) + (Dhpg)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 41113:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpg - D-Tyr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 16593 - 84275:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpg - D-Tyr) + (Dhpg) + (D-Hpg - D-Hpg - Tyr) + (Dhpg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

Nocox_13350: Hpg - Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (10..398): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFHLGLLCK
Nearest Stachelhaus code(s):: Hpg DAFHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1038..1425): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTVAAVCK
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Nocox_13355: Dhpg

Search NORINE for peptide: strict or relaxed

AMP-binding (465..853): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: dhpg (Dhpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DAYNAGTLCK
Nearest Stachelhaus code(s):: Dhpg DAYNAGTLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Nocox_13360: Hpg - Hpg - Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (459..853): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DIFHLGLLCK
Nearest Stachelhaus code(s):: Hpg DIFHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1951..2348): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DALHLGLLCK
Nearest Stachelhaus code(s):: Hpg DALHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3471..3863): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTVAAVCK
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Nocox_13365: Dhpg

Search NORINE for peptide: strict or relaxed

AMP-binding (471..871): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: dhpg (Dhpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DPYHGGTLCK
Nearest Stachelhaus code(s):: Dhpg DPYHGGTLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor