LOCUS       BGC0002345              2976 bp    DNA     linear   BCT 12-SEP-2019
DEFINITION  Pseudomonas fluorescens isolate PS619 genome assembly, contig:
            PS619_39, whole genome shotgun sequence.
ACCESSION   BGC0002345
VERSION     BGC0002345
KEYWORDS    .
SOURCE      Pseudomonas fluorescens
  ORGANISM  Pseudomonas fluorescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:22:17
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..318
                     /EC_number="1.4.99.5"
                     /codon_start=1
                     /gene="hcnA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniProtKB:O85226"
                     /locus_tag="PS619_05843"
                     /product="Hydrogen cyanide synthase subunit HcnA"
                     /protein_id="VVN46760.1"
                     /sec_met_domain="Fer2_4 (E-value: 7.2e-24, bitscore: 71.5,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHCQERTFDIQPLAQADMTVHINGQAVTAAIGETVLSVIQSLGVR
                     QIARNDHDQITGAYCGMGVCQCCLVKINGRHKRRACQTVVRDGMQIETQVNRITAQEVI
                     V"
     protocluster    1..2976
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[0:2976]"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..2976
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     cand_cluster    1..2976
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..2976
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002345"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..2976
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="hydrogen-cyanide"
                     /region_number="1"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..318
                     /gene="hcnA"
                     /locus_tag="PS619_05843"
     gene            315..1724
                     /gene="hcnB_2"
                     /locus_tag="PS619_05844"
     CDS             315..1724
                     /EC_number="1.4.99.5"
                     /codon_start=1
                     /gene="hcnB_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniProtKB:O85227"
                     /locus_tag="PS619_05844"
                     /product="Hydrogen cyanide synthase subunit HcnB"
                     /protein_id="VVN46770.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.4e-23, bitscore:
                     71.2, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 3.1e-11, bitscore:
                     31.4, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLQPVIVGGGPAGMAAAIELARHGVRCTLLEEASRLGGVVYRGP
                     LRDGVQLDYLGPRYSEALGKLHGDFQEQAGLIDVRLNHRVVGAEGTRALVVLDGDEQLH
                     EIEYPQLLLSAGCHERSVPFPGWTVPGVIMLGGLQLQIKSGVVKPQGPVIIAGTGPLLP
                     LVATQLHAAGVSVAGVYEACAFGKIARESLALLNKPQLFLDGLSMLAYLKLHGIPMNYG
                     WGVVEAHGEGELKSVSVAPYSATWEPDMSRVERFEAKTLAVGYGFIPRTQLSQQMGLDH
                     GFSDDGYLRANANVWQQSNEPHVHLAGDMGGIRGGEAAMLAGKIAATSILLQRGVLEEE
                     LARVRRDRYLSKLKAIVRFRAAVDRYTERGVGQTALPAADTVICRCEHATRADIDLALE
                     QGVQDIASLKMRTRVSMGDCQGRMCVGYCSDRLRQATGRKDVGWLRPRFPIDPIPFSAF
                     QSVDTEAAAHE"
     gene            1717..2976
                     /gene="hcnC"
                     /locus_tag="PS619_05845"
     CDS             1717..2976
                     /EC_number="1.4.99.5"
                     /codon_start=1
                     /gene="hcnC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103:FAD dependent oxidoreductase (Score: 291.8;
                     E-value: 1.3e-88)"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:UniProtKB:O85228"
                     /locus_tag="PS619_05845"
                     /product="Hydrogen cyanide synthase subunit HcnC"
                     /protein_id="VVN46780.1"
                     /sec_met_domain="DAO (E-value: 8.9e-67, bitscore: 213.7,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKFYDVVIAGGGVIGASCAYQLSKRKNLKIALIDAKRPGNATRA
                     SAGGLWAIGESVGLGCGVIFFRMMSANRKRETQGAAVAVDASTPHILPESFFDFALQSN
                     ALYPALHRELKDNHGMDFKFEKTGLKFVIYDDEDRLYAEHIVGCIPHLADQVRWLDQAA
                     LRESEPSVSHEARGALEFLCDHQVSPFRLADAYMEGARQNGVDIFVNTNVTGVLHHGSR
                     VTGVQTAEEGVFHCKTLINASGAWAADLSEWATGIRIPVKPVKGQILLTERMPKILNGC
                     LTTSDCYVAQKDNGEILIGSTTEDKGFDVTTTYPEIAGLVQGAVRCLPELKDVNLKRTW
                     AGLRPGSPDELPILGPMRGVEGYLNACGHFRTGILTSAITGVLLDKLVNDEPLPLDITP
                     FLADRFEVAPVKQALELELA"
ORIGIN
        1 atgcactgcc aagaaagaac tttcgatatc cagccgttgg cgcaggcgga catgaccgtc
       61 cacatcaacg gtcaagcggt caccgccgcc atcggcgaaa ccgtactcag cgttattcaa
      121 tcactcggcg tacgccagat tgcccgcaac gatcacgatc agattaccgg cgcctattgc
      181 ggcatgggcg tgtgtcagtg ctgcctggtg aaaatcaatg gtcggcacaa acgccgtgcc
      241 tgtcagaccg tcgtgcgcga cggcatgcag atcgaaaccc aagtcaaccg catcaccgcg
      301 caggaggtga tcgtatgagc ctgcaaccgg tcatcgtcgg cggtggcccg gcgggaatgg
      361 cggcggccat cgaactggcg cgccacgggg tgcgctgcac cttgctcgaa gaagcttcac
      421 gccttggcgg tgtggtctat cgcgggccgt tgcgtgacgg cgtgcaactg gactatctcg
      481 gcccgcgtta ctccgaagcg ctgggcaaac tgcacggtga ttttcaggag caggccgggt
      541 tgatcgacgt gcgcctcaac catcgcgtgg tcggcgctga aggcacccgc gctttggtgg
      601 tactcgatgg cgacgagcaa ctgcacgaaa tcgaatatcc gcaattgctg ttgtccgccg
      661 gatgccatga acgcagcgtg ccatttccgg gctggacggt gccgggtgtg attatgctcg
      721 gcggtctgca actgcaaatc aaaagtggcg tggtcaagcc gcaagggccg gtgatcatcg
      781 ccggcaccgg gccgttgttg ccgctggtgg cgacgcagtt gcatgccgcg ggcgtcagcg
      841 tcgcgggcgt gtatgaagct tgcgcgttcg gcaagattgc ccgcgaaagt ctggcgctgc
      901 tgaacaagcc gcagctgttt ctcgacggtt tgagcatgct cgcctacctg aaactgcacg
      961 gtatcccgat gaactacggc tggggcgtgg tcgaagccca cggcgagggc gagctgaaaa
     1021 gcgtcagcgt cgcgccgtac tcggcgacgt gggagccgga catgagccgc gtcgagcgct
     1081 tcgaagccaa gaccctcgcg gtcggctacg gctttattcc ccgcacgcaa ctgagccagc
     1141 agatgggcct ggatcatggc ttcagcgacg acggttatct gcgcgccaat gcgaatgtct
     1201 ggcagcaaag caacgaacct cacgtgcatc tggccggcga catgggcggg attcgcggcg
     1261 gtgaggcggc gatgctcgcc ggcaagatcg ccgcaacctc gattctgctg caacgtggcg
     1321 tgctcgaaga ggaattggct cgggtgcgcc gcgaccgcta cttgagcaaa ctcaaagcca
     1381 tcgtgcgctt tcgcgccgcc gtcgatcgct acaccgaacg cggcgtcggc cagaccgcat
     1441 tgcccgccgc cgacacggtg atctgccgtt gcgagcacgc gacccgcgcc gacatcgacc
     1501 tggcgctgga gcagggcgtg caagacatcg ccagcctgaa aatgcgcacc cgcgtgagca
     1561 tgggcgattg ccagggccgg atgtgcgtcg gctactgcag cgaccgcctg cgccaggcca
     1621 ccggacgcaa ggacgtcggt tggctgcgcc cgcgttttcc gatcgatccg attccttttt
     1681 ccgcgttcca gtctgtcgac acggaggccg ctgcccatga gtaagttcta tgacgtggtc
     1741 atcgccggtg gtggcgtgat cggggcgtcc tgcgcctatc aattgtccaa gcgcaaaaac
     1801 ctcaaaatcg cgctgattga tgccaagcgc ccgggcaacg cgacccgtgc gtcggccggt
     1861 ggcttgtggg cgatcggcga gtcggtcggc ctcggttgcg gggtgatttt cttccgcatg
     1921 atgtcggcca accgcaagcg cgaaacccaa ggcgcggcgg tggcggtaga cgcgagcacg
     1981 ccgcacattc tgccggagtc gtttttcgat tttgccctgc agtccaacgc cctgtacccg
     2041 gcgttgcacc gagagctgaa agacaatcac ggcatggatt tcaagttcga aaagaccggg
     2101 ttgaaatttg tcatctatga cgacgaagac cggctctacg ctgagcacat cgtcggctgc
     2161 attccgcacc tggccgatca ggtgcgctgg ctcgatcaag cggcgctgcg cgaatccgag
     2221 ccgagcgtca gccatgaagc acgcggggcg ctggagtttc tctgcgacca tcaggtcagc
     2281 ccgttccgcc tcgccgacgc ttatatggaa ggcgcgcggc agaacggcgt cgacatcttc
     2341 gtcaacacca acgtcaccgg cgtgttgcac cacggcagcc gcgtgaccgg tgtgcagacg
     2401 gctgaggagg gcgtgttcca ctgcaagacg ctgatcaacg ccagcggtgc ctgggcggcg
     2461 gacttgagcg aatgggcgac ggggattcgc atcccggtga aaccggtgaa aggccagatt
     2521 ctactgaccg agcgcatgcc gaaaatcctc aacggctgcc tgaccaccag cgactgctac
     2581 gtggcgcaga aagacaacgg cgagatcctg atcggcagca ccaccgaaga caaaggcttc
     2641 gacgtcacca ccacctaccc ggaaatagcc ggactggtgc aaggcgcggt gcggtgtctg
     2701 ccggagctga aagacgtcaa cctcaagcgt acttgggcgg ggttgcgtcc ggggtcgccg
     2761 gatgagttgc cgattctcgg gccgatgcgt ggggtggagg gttatctgaa tgcctgcggg
     2821 cacttccgca ccgggattct gacctcggcg attaccggtg tgttgctgga caaactggtc
     2881 aacgacgaac cgctgccgct ggatatcaca ccgtttctgg cagatcggtt tgaggtggcc
     2941 ccggtcaaac aagcgctgga gttggaactg gcctga
//
