LOCUS       BGC0002346             11935 bp    DNA     linear   BCT 01-JUL-2019
DEFINITION  Saccharopolyspora sp. strain KY21 kyamicin biosynthetic gene
            cluster, complete sequence.
ACCESSION   BGC0002346
VERSION     BGC0002346
KEYWORDS    .
SOURCE      Saccharopolyspora sp
  ORGANISM  Saccharopolyspora sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Saccharopolyspora.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:22:23
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11935
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11935
                     /db_xref="taxon:33915"
                     /host="Tetraponera penzigi"
                     /mol_type="genomic DNA"
                     /organism="Saccharopolyspora sp."
                     /strain="KY21"
     misc_feature    1..11935
                     /note="kyamicin biosynthetic gene cluster"
     protocluster    1..11935
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5709:8907]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5710..8907
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..11935
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11935
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002346"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11935
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             complement(15..143)
                     /gene="allorf_00015_00143"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="allorf_00015_00143"
                     /protein_id="allorf_00015_00143"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MDFGRWISVDPFGALRCLPKVEKELSSRSLDGPPRGWVAENG"
     CDS_motif       complement(102..143)
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MDFGRWISVDPFGA"
                     /locus_tag="allorf_00015_00143_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(15..101)
                     /prepeptide="core"
                     /locus_tag="allorf_00015_00143_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-6.10"
                     /molecular_weight="3040.4"
                     /monoisotopic_mass="3038.6"
                     /core_sequence="LRCLPKVEKELSSRSLDGPPRGWVAENG"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MDFGRWISVDPFGA"
                     /leader_location="[101:143](-)"
                     /alternative_weights="3058.5"
                     /alternative_weights="3076.5"
     CDS             261..395
                     /gene="allorf_00261_00395"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="allorf_00261_00395"
                     /protein_id="allorf_00261_00395"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MIERTVVEVSAQRFDPLTRLSKNCARSSADPGKNPRSGDAHLLS"
     CDS_motif       261..290
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MIERTVVEVS"
                     /locus_tag="allorf_00261_00395_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       291..395
                     /prepeptide="core"
                     /locus_tag="allorf_00261_00395_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-7.20"
                     /molecular_weight="3558.9"
                     /monoisotopic_mass="3556.9"
                     /core_sequence="AQRFDPLTRLSKNCARSSADPGKNPRSGDAHLLS"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MIERTVVEVS"
                     /leader_location="[260:290](+)"
                     /alternative_weights="3576.9"
                     /alternative_weights="3595.0"
                     /alternative_weights="3613.0"
                     /alternative_weights="3631.0"
                     /alternative_weights="3649.0"
     gene            611..1396
                     /gene="kyaR1"
     CDS             611..1396
                     /codon_start=1
                     /gene="kyaR1"
                     /gene_functions="regulatory (smcogs)
                     SMCOG1041:transcriptional regulator, SARP family (Score:
                     266.7; E-value: 8.2e-81)"
                     /gene_kind="regulatory"
                     /note="Streptomyces antibiotic regulatory protein"
                     /product="KyaR1"
                     /protein_id="QDF63352.1"
                     /transl_table=11
                     /translation="MTKPLSFHVLGPLTVGSGGRCLDVPGSRQRILLAALLVKFNQVVC
                     VDELVDRIWDSRPPCRPRRALHTCLTRLRHAIADASGAPDLIRTSSAGYTLQASPETLD
                     FARFTELLEQARAAEAAHDRVRESAALGEALALWRGERALPDVASDSLHRDVVPGIHQQ
                     RIEALERRGELELELGHHRELIGELRALTSAHPFHERFWQQLLLALYRCGRKVEALLAY
                     AELTSRIRDGLGVEPGPQLRDLHLAILRDDQVPPLHTAS"
     gene            complement(1381..2283)
                     /gene="kyaorf11"
     CDS             complement(1381..2283)
                     /codon_start=1
                     /gene="kyaorf11"
                     /gene_functions="other (smcogs) SMCOG1188:hypothetical
                     protein (Score: 132.7; E-value: 1.5e-40)"
                     /gene_kind="other"
                     /note="DedA-family protein"
                     /product="Kyaorf11"
                     /protein_id="QDF63353.1"
                     /transl_table=11
                     /translation="MPSPADWLAQLPPLAAYGLLAGLVFTEGILVISPFVPTLGPLLVA
                     GALVYAGVLEPWAVVLAASAGAVCGDALGHWTGRHLGPRLRETRFARRVPRAWDRASEL
                     FRRSGGPALVPCRFVPLVRSAAPHLIGASGLPYRRMAPWSALAGVVWACGEGSVGYLAG
                     ASYSRLAGAFGAIPAVAVLVLVVLGVVYWRRRGSGGADGSDRSADAADRADPEGAGPEG
                     AGPEGAGSEGAAPDRAGPEDADPEGAGPAGTRPDRADPVGAGAGAVEPGVADAGGADRP
                     GRAGPRYRAQRRPGRYDAV"
     gene            complement(2283..2963)
                     /gene="kyaL"
     CDS             complement(2283..2963)
                     /codon_start=1
                     /gene="kyaL"
                     /note="phosphatidylethanolamine methyl transferase"
                     /product="KyaL"
                     /protein_id="QDF63354.1"
                     /transl_table=11
                     /translation="MDPVQTSSSTSPGRFAFSREAFRSFRVTGAIAPSSRRLAARLAAP
                     LLPPQHRPPARVLEVGAGSGVVTRELADRIGPADRLDVVEINPHYVRMLDTALRTEFAL
                     SAVADRIALHEVSVLDAPLEPRYDVVVSGLPFTNFEPEQVRALLDRHLELLVPGGHLTF
                     FGYLGTQRARALVSGPTESRRHRAAMEVEREFVERYGLGRSTVWWNLPPAHVWHLRAPA
                     AEDR"
     gene            3088..4206
                     /gene="kyaK"
     CDS             3088..4206
                     /codon_start=1
                     /gene="kyaK"
                     /gene_functions="regulatory (smcogs) SMCOG1048:sensor
                     histidine kinase (Score: 179.3; E-value: 2.1e-54)"
                     /gene_kind="regulatory"
                     /note="histidine kinase"
                     /product="KyaK"
                     /protein_id="QDF63355.1"
                     /transl_table=11
                     /translation="MTAVPPRTRWWVEAALLVAVSLLSGQEAYVRAGGVVLSPQVGAVL
                     LAGIALAWRGRNLAVAAVPAVLVAAVLGTVLPLLVVLYSCAKRGRLVVAAVCAVAAMGG
                     NLLQPQHSLWGTRVYGPLLLLVAVVALGMWAGGHRRLVASLAAQVDQLRVERELRAEQA
                     RLTERGRVAAELHDSLAHSLSVLALHTGALQRHSAELPPQVADRIDLLRATSTEALRDL
                     RDVLAGLRSPDGGGVPEPRRMRDLEALFAEARAAGQPVAAEVSGDAAELPFSHRLAIYR
                     LVQEALTNARKHAPGSAVRVRVRHGPPESTVDVENDVGAPVERDSPSGYGLVGLAERVD
                     ALHGTLEHGPSGTGGWRLSARIPAPAHSSGAA"
     gene            4203..4853
                     /gene="kyaR"
     CDS             4203..4853
                     /codon_start=1
                     /gene="kyaR"
                     /gene_functions="regulatory (smcogs) SMCOG1016:LuxR family
                     DNA-binding response regulator (Score: 160.7; E-value:
                     4.7e-49)"
                     /gene_kind="regulatory"
                     /note="response regulator"
                     /product="KyaR"
                     /protein_id="QDF63356.1"
                     /transl_table=11
                     /translation="MIRTLIVDDDTLVRLALADILADAPGITVVGQAGDGLSAVREAAA
                     GQVDVVLMDVRMPELDGIQATERLRALPNPPQVVTLTTFDLDEYVYGSLTAGAAGFLLK
                     DSEPAEIVRAVQVVASGQAMLHPAAARRLVDHYHRGSGPDSAAARAKIERLTPRERDVL
                     GRLATGDGNAEIASALGMRESTVKAHVSRILAALDVGNRVQAALCAWAADQSG"
     regulatory      4917..4943
                     /note="SARP binding site"
                     /regulatory_class="other"
     gene            4998..5369
                     /gene="kyaN"
     CDS             4998..5369
                     /codon_start=1
                     /gene="kyaN"
                     /note="catalyses lysino-alanine formation"
                     /product="KyaN"
                     /protein_id="QDF63357.1"
                     /transl_table=11
                     /translation="MQHSDVPVIYPDVDVIRRIQELVVLCSLLPPDGKLREVLQLALAL
                     NEEPTLARLTPVTDLHPFATHKWLEQLWSPEGLPEQEKEVVAWQNENDNMGRALVELKN
                     AEAQLGFALVAQLPAEKSE"
     gene            5402..5638
                     /gene="kyaA"
     CDS             5402..5638
                     /codon_start=1
                     /gene="kyaA"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="precursor peptide"
                     /product="KyaA"
                     /protein_id="QDF63358.1"
                     /transl_table=11
                     /translation="MSDTILRQAVIDAEFRSALIADPAAFGVEPAELPDAVEAVDQESL
                     DFWTKGIAANEVVACASTCSAGPFTFACDGSTK"
     CDS_motif       5402..5482
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MSDTILRQAVIDAEFRSALIADPAAFG"
                     /locus_tag="kyaA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       5483..5638
                     /prepeptide="core"
                     /locus_tag="kyaA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-6.60"
                     /molecular_weight="5105.6"
                     /monoisotopic_mass="5102.4"
                     /core_sequence="VEPAELPDAVEAVDQESLDFWTKGIAANEVVACASTCSAGPFT
                     FACDGSTK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MSDTILRQAVIDAEFRSALIADPAAFG"
                     /leader_location="[5401:5482](+)"
                     /alternative_weights="5123.6"
                     /alternative_weights="5141.6"
                     /alternative_weights="5159.6"
                     /alternative_weights="5177.6"
                     /alternative_weights="5195.7"
     gene            5710..8907
                     /gene="kyaM"
     CDS             5710..8907
                     /codon_start=1
                     /gene="kyaM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     670.6; E-value: 6.4e-203)"
                     /gene_kind="biosynthetic"
                     /note="LanM; class II lanthionine synthetase"
                     /product="KyaM"
                     /protein_id="QDF63359.1"
                     /sec_met_domain="DUF4135 (E-value: 4.5e-67, bitscore:
                     216.3, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 9.3e-64, bitscore:
                     204.8, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQTDRALDLAARASNLDERLHVVTALRDSGRPLAAAPPDAVERWR
                     LDRVAGRLAGKFAQEAENRARPARHTAEELAAVLTAYRAVELDPAGPGLPELLEELHAA
                     WLPTYRDAVLGWDPAHSDPRATWRETDVYYGELATACEPFLVELGRRLEKVRAELPESI
                     GPDLVDDLQRHLHGRFALALAWVVEAEANLHRARRGIDRERAGEDDYLAYLDATFADSA
                     AYHEFFLEYPVLGRWLAHLTGLIAGYGADLLRNLAADAARLGPELFGRSIVAFRSLRLG
                     TGDYHAGGRSVAVVTAELDDGGTGTFYCKPRCLRSEAGVQAVLDRLRERGVVEFASRPV
                     LVRDGYGYEAAIPTGRNETGTAEAAAHVYRELGGHLALFYVLGGSDLHFENIIVADGHV
                     HVCDGETVFDASPLGRGGSGTVQDSVFKTGLLEWPRAGTADGPEQMRLSGCTGGESYEI
                     PMPVPQIVGGRLGFGAAVRNRSGVRVELDATNRVFLHGELVHPQDHLESIVDGFDRVHE
                     WLERNADEAVALVSEAFRGASARFLNWSTQIYAQLIASARHPKCLADPLEVDLLLNTVR
                     TFPREWDRDAVLSARETASMWRLDVPVFTVEVGDDHLVHDRAERLPTALDGSPLERAAE
                     RIRSLTAKNRAQQARYIAASLSNDEISSPAFVATALDYAVRVGERLCSMELEPPAPAPW
                     KSFGRVDGVLESVDIDADLYTGTAGVALFLAYLDDVVPRPEFRRAARRALRHVLDTPPQ
                     RTGAFADVGGRLYLLTHLHRLWGEQELVDEAVRACDGLAELIDADTHLDLLHGAAGMIP
                     VLLALEPLLGDQALDLAHRCADHLLRRARDRDGAVSWPSNTPEHARDDMTGFAHGAAGI
                     GWALVLLGTRTGRDECVAAGRSAFAYEARHFDLDEQDWYDLRYDTGQVAKNGHHYSNAW
                     CNGAAGIGLSRIAAWSLLGADDEELLREARHALGATVRNFSRLKNHTLCHGTSGNSELL
                     LRYALLRDEPAFRLEANVQVREMWRSLDDAEHGLGEQSADFFPGLMMGISGYGMHFLRL
                     AHPERVPSVLMLDPPRP"
     gene            9015..9923
                     /gene="kyaX"
     CDS             9015..9923
                     /codon_start=1
                     /gene="kyaX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03798"
                     /gene_kind="biosynthetic-additional"
                     /note="alpha-ketoglutarate dependent non-heme
                     Fe(II)-dependent hydroxylase"
                     /product="KyaX"
                     /protein_id="QDF63360.1"
                     /sec_met_domain="TIGR03798 (E-value: 4.9e-11, bitscore:
                     32.3, seeds: 22, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVEACKSFLRATRQQPELGQQLKAVTGMEEMIALGGRHGFDFTA
                     QELAAGSAELGAETTPEPAADEPAPPRGTAFYHHEYDLADVPELAPLIDELPLLKVRPP
                     VDLAAFRARFRADDLESTSKSPSDPEFQEWNRRMMAAHWQDPAGGGARRDFHLVNLDEH
                     VEHPGYPAYFAAKTRAIRLLEGVFGDEIRLSGSMWYPPNSYRLWHTNENQPGWRMYVVD
                     LDDDFPEGGGTSFFRYQNPGTGEIVTLPERRRIVRFFKAEQDPARLFWHCIVNPTERHR
                     WSFGFVVPEDWQDHLGSLRAA"
     gene            9971..10954
                     /gene="kyaT"
     CDS             9971..10954
                     /codon_start=1
                     /gene="kyaT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 226.1; E-value:
                     8.1e-69)"
                     /gene_kind="transport"
                     /note="ABC transporter ATP binding subunit"
                     /product="KyaT"
                     /protein_id="QDF63361.1"
                     /transl_table=11
                     /translation="MRRLRRGDDHHRPEDAPAIVARDVHKSYRGTHAVRGVDITVARGE
                     TFGFLGPNGAGKSTTIAMMCTLALPDAGRIEIAGYDVVRDAHQVRRRLGLVFQESTLDP
                     DLTTAENLGFHADLYAMPRALVAERVDSMLDLVGLRERRADRVATFSGGMRRRLEIARG
                     LLHRPSVLFLDEPTVGLDPQTRAEIWTYLHRIAEQEETTVFLTTHYLDEAERCDRIAII
                     DAGRIVAQGTPAELKSVIGADRIALRTGDDDAAVRVLRERFDLEATTADGGVQVQVSDG
                     ARFVPVLCSGLNVPIFEVTVTRPSLDEVFLHYTGRAIRDVPGVPAP"
     gene            10980..11864
                     /gene="kyaH"
     CDS             10980..11864
                     /codon_start=1
                     /gene="kyaH"
                     /gene_functions="transport (smcogs) SMCOG1065:ABC-2 type
                     transporter (Score: 134.5; E-value: 5.8e-41)"
                     /gene_kind="transport"
                     /note="ABC transporter membrane subunit"
                     /product="KyaH"
                     /protein_id="QDF63362.1"
                     /transl_table=11
                     /translation="MTSTAPPRTAAPSEQDAERSGRLAREWRAGSMVWRRELLHFLRDR
                     TRVAVSLLPPFLFLYVLGIGLSRLLADGAGGARTAEDYLVFLFPGVLVMAAQAPAISVG
                     ASIVWDRQTGFMREMLAAPVFRGTLLLGKCLGGATVATAQSAVVLLTAGLVGVPYDPVL
                     FAVLLAEIAIAALSLTVLAAGVAVAIKRVQTFNTVLSVLITPLVFLSGMMFPISAMPGW
                     MVALTVVNPLTYAVDAMRHAVLGRLGQEHSSVIFSPVEWAGWEVPPPVEIAVVLGFSVL
                     ALVAISRRFARTG"
ORIGIN
        1 ggagaacacc cctcttatcc attctcggcc acccaccccc ggggcggccc gtcgaggctc
       61 ctgctcgaca actctttttc gaccttgggc agacacctta gtgccccgaa cgggtcaacc
      121 gaaatccacc gaccgaaatc catccgcgca ttcgttcgaa tagtccgatt tgcactttac
      181 tcgccgccac ctggggactc ggacattcga accgagattc gcatagcggt cacccaccgg
      241 cggagccggt tgcacggaca gtgattgagc gaaccgtggt cgaagtctcc gcacagcggt
      301 tcgacccgtt aacacgatta tcgaaaaatt gcgcgcgcag ttccgccgat ccggggaaaa
      361 acccccgatc cggggacgcc cacctacttt cgtaagaccc cgtacgcagc attgccggcc
      421 gttgaagatc gatgctaaaa agtgaaaatc aactcccggg ccggccgctc gcgcgccgcc
      481 gatgactcgg gaatcccggc cgcgatctgg ggaatcgccg cgccgaccgc cggtcgaacg
      541 cgacccagcc cgcgcaccgc cgggagccga ccggcatgcc gggaccgcac cgcacaactg
      601 ggggctccac gtgacgaaac cgctgtcgtt ccacgtcctc ggcccgctca ccgtcgggag
      661 cggcgggcga tgtctcgacg tacccggatc caggcagcgc atcctgctcg ccgcgctgct
      721 ggtcaagttc aaccaggtgg tgtgcgtcga cgagctcgtg gaccggatct gggactcccg
      781 gccgccctgc cggccgcgcc gggcgctgca cacctgcctg acccggctcc ggcacgccat
      841 cgcggacgcg tccggcgcgc cggacctcat ccgcacctcc agcgccgggt acacgctcca
      901 ggcgtccccg gagaccctcg acttcgcccg cttcaccgag ctgctggagc aggcgcgcgc
      961 cgcggaggcc gcccacgacc gggtccgcga atccgccgcc ctcggcgagg cgctggcgct
     1021 gtggcgcggg gagcgcgcgc taccggacgt ggcctcggac tcgctgcacc gcgacgtggt
     1081 gcccgggatc caccagcagc gcatcgaagc gctggagcgg cgcggcgaac tggagctgga
     1141 actgggccac caccgcgagc tcatcggcga actgcgggcg ttgacctccg cacatccgtt
     1201 ccacgagcgg ttctggcagc agctgctcct cgcgctctac cgctgcgggc ggaaggtgga
     1261 ggcgctgctg gcctacgccg agctgacctc gcgcatccgc gacggcctcg gcgtggagcc
     1321 cggaccgcag ctgcgcgacc tgcacctggc gatcctgcgc gacgaccagg tgccgccgct
     1381 tcacaccgcg tcgtagcggc cgggacgccg ctgcgcgcgg taccgcggcc ccgcgcggcc
     1441 gggacggtcc gctccccccg cgtccgcgac gcccggttcc acggccccgg cgcccgctcc
     1501 gaccggatcg gcccggtccg ggcgcgtgcc cgccggccca gcgccttccg gatcagcgtc
     1561 ctccggaccc gcacggtccg gagccgcgcc ttccgaaccc gcgccctccg gacccgcgcc
     1621 ctccggaccc gcgccttccg gatccgcgcg gtccgccgca tcggccgacc ggtccgagcc
     1681 gtccgcccca ccggagccgc gccgtcgcca gtacacgacc cccagcacca ccagcacgag
     1741 caccgccacg gccgggatcg cgccgaacgc cccggccagc cgcgagtacg acgccccggc
     1801 caggtagccc acgctgcctt ccccgcacgc ccacaccacc ccggcgagcg cgctccacgg
     1861 tgccatccgg cggtagggca ggccggacgc gccgatcagg tgcggagccg cggaccgcac
     1921 cagcggcacg aaccggcacg gcaccagcgc gggcccgccg ctgcggcgga acagctcgct
     1981 cgcgcggtcc cacgcgcgcg ggacgcgacg cgcgaaccgg gtctcccgca gccgggggcc
     2041 gaggtggcgc ccggtccagt ggccgagcgc gtccccgcac accgcgccgg cggaagcggc
     2101 cagcaccacc gcccacggtt ccagcacccc cgcgtagacg agcgcgcccg cgacgagcag
     2161 cggccccagc gtgggcacga acgggctgat caccaggatc ccttcggtga acaccagccc
     2221 ggcgagcaac ccgtaggcgg ccagcggcgg cagttgcgcc agccagtcgg ccggtgacgg
     2281 catcagcggt cctccgccgc gggtgcgcgc aggtgccaga cgtgcgcggg cggcaggttc
     2341 caccacaccg tcgagcggcc gaggccgtag cgctcgacga actcgcgctc cacctccatc
     2401 gcggcccggt gccgccgcga ctcggtcggg ccgctcacca gggcgcgggc ccgttgggtg
     2461 ccgaggtagc cgaagaaggt gaggtgcccg cccggaacga gcagttcgag gtgccggtcc
     2521 agcagcgccc gcacctgctc gggttcgaag ttggtgaacg gcagcccgga gacgaccacg
     2581 tcgtagcgcg gttccagcgg cgcgtccagc accgagacct cgtgcagcgc gatccggtcg
     2641 gcgacggcgg agagcgcgaa ctcggtgcgc agcgcggtgt cgagcatccg cacgtagtgc
     2701 gggttgatct ccacgacgtc cagccggtcg gcggggccga tccggtcggc gagctcccgc
     2761 gtcaccaccc cgctgcccgc cccgacttcg agcaccctgg ccggtggccg gtgctgcggc
     2821 ggcagcagcg gcgcggcgag ccgggcggcg agccgtctgc tgctgggggc gatcgccccg
     2881 gtgacgcgga agctgcggaa cgcctcgcgg gagaaggcga accgtccggg cgacgtgctg
     2941 ctgctggtct gcactgggtc cacgtcgttg cacgctactg gcgggatcaa cgggcaccat
     3001 cggtctcatg gccacggaga tccggtacga aagtaggggg gcggcggctc cctatgctcg
     3061 tccgtcgtgg cgacgaggag caacccggtg accgcggtac cgccgcgcac gcggtggtgg
     3121 gtggaggcgg cgctgctcgt cgcggtctcg ctgctgtccg ggcaggaggc ctacgtccgc
     3181 gccggcggcg tggtgctgag cccgcaggtc ggcgcggtgc tgctggccgg gatcgcgctc
     3241 gcgtggcgcg ggcggaacct ggcggtcgcg gcggtacccg cggtcctggt ggccgcggtg
     3301 ctggggacgg tgctgccgct gctggtggtg ctgtactcct gcgcgaagcg cggcaggctg
     3361 gtggtggcgg cggtgtgcgc ggtcgcggcg atgggcggga acctgctgca gccgcagcac
     3421 tcgctgtggg gcacccgggt gtacgggccg ctgctgctgc tggtcgcggt ggtggcgctg
     3481 ggcatgtggg cgggcgggca ccggcggctg gtggcgagcc tggccgcgca ggtcgaccag
     3541 ctgcgggtgg agcgggagct gcgcgcggag caggcgcggt tgaccgagcg cggccgggtc
     3601 gcggccgagc tgcacgactc gctggcgcac tcgttgagcg tgctggcgct gcacaccggt
     3661 gcgctgcaac ggcattcggc cgagctgccc ccgcaggtcg cggaccgcat cgacctgctc
     3721 cgggcgacgt cgacggaggc gctgcgcgac ctgcgggacg tgctggcggg actgcgctcg
     3781 ccggacggcg gcggggttcc ggagccgcgc cggatgcgcg atctggaggc gctgttcgcg
     3841 gaggcgcggg cggccgggca gccggtggcg gccgaggtct ccggtgacgc ggcggagctg
     3901 ccgttcagcc accggctggc catctaccgg ctggtgcagg aagcgctgac gaacgcgcgc
     3961 aagcacgcac cgggctcggc cgtgcgggtc cgggtccggc acggtccgcc ggagtccacc
     4021 gtggacgtgg agaacgacgt gggcgcgccg gtggagcggg actcgcccag cggctacggc
     4081 ctggtggggc tggccgaacg ggtcgacgcg ctgcacggca ccctggaaca cggcccttcg
     4141 ggcaccggcg gctggcggct gtcggcgcgc atcccggcac ccgcccactc ctcgggagca
     4201 gcatgatccg cacgctgatc gtggacgacg acacgctggt ccgcctggcg ctcgccgaca
     4261 tcctcgccga cgcaccgggg atcaccgtgg tcgggcaggc cggggacggg ctgagcgcgg
     4321 tccgggaggc cgcggccggc caggtcgacg tggtgctgat ggacgtgcgg atgccggagc
     4381 tggacggcat ccaggccacc gagcgcctgc gcgcgctgcc gaacccgccg caggtcgtca
     4441 cgctcaccac cttcgacctc gacgagtacg tgtacggctc gctgaccgca ggcgccgccg
     4501 ggttcctgct caaggacagc gaacccgccg agatcgtgcg cgccgtgcag gtcgtcgcga
     4561 gcgggcaggc gatgctgcac ccggcggcgg cgcggcggct cgtcgaccac taccaccggg
     4621 gcagcggacc ggattccgcc gcggcgcgcg cgaagatcga acgcctcacc ccccgggagc
     4681 gggacgtgct cggccggttg gcgacgggcg acggcaacgc cgagatcgcg agcgcgctcg
     4741 gcatgcggga gagcacggtg aaggcgcacg tgagccgcat cctcgccgcg ctcgacgtgg
     4801 gcaaccgggt gcaggccgcc ctctgcgcct gggcggccga ccagtccggc tgacggaacc
     4861 gcgaacctcc tgccgcggcg gcggatcccg ctgccgccac gccggttctc ctgcggtgaa
     4921 acagctttga aggcggactg aaaccaccgc cccctagcgt cggtcgcgtt gagcctccga
     4981 cggattggga gcggtcgatg cagcactcgg atgttccagt catctacccg gacgtggacg
     5041 tgatccggcg aatccaggaa ctcgtggtcc tgtgctcgct gctgccgccg gacggcaagc
     5101 tgcgcgaggt gctgcagctc gccctcgcgc tgaacgagga gcccaccttg gcccggctca
     5161 cgccggtcac cgacctgcac cccttcgcga cccacaagtg gctggagcag ctgtggtcgc
     5221 cggagggact gccggagcag gagaaggagg tcgtcgcctg gcagaacgag aacgacaaca
     5281 tgggccgagc gctcgtggag ttgaagaacg cggaggcgca gctgggtttc gccctcgtgg
     5341 cgcagctgcc cgccgagaag tccgaatgat ccgaacgcac accagcatcc aggaggagat
     5401 catgtccgac accattcttc gccaagccgt catcgacgcc gagttccgct ccgcgctcat
     5461 cgcggacccc gccgccttcg gcgtggagcc tgccgagctg cccgacgcgg tcgaggccgt
     5521 cgaccaggag tccctggact tctggaccaa ggggatcgcg gccaacgagg tcgtcgcctg
     5581 cgccagcacc tgcagcgccg gcccgttcac gttcgcgtgc gacggctcga ccaagtgagc
     5641 tgagcatcga cgcggtgagc cttcgggctc gccgcgttcc tcgcccacca ccgaccggga
     5701 caggtgttga tgcagaccga ccgagcactc gatctggccg cccgcgcgag caacctggac
     5761 gagcggctgc acgtcgtcac cgcgctgcgc gactcggggc ggccgctcgc ggcggcaccg
     5821 cccgacgcgg tcgagcggtg gcggctggac cgcgtcgccg gacggctcgc cgggaagttc
     5881 gcgcaggagg ccgagaaccg ggcgcggccc gcccggcaca ccgccgagga gctcgccgcg
     5941 gtgctcaccg cctaccgcgc cgtcgagctg gacccggccg gccccggcct gccggagctg
     6001 ctggaggagc tgcacgcggc ctggttgccg acctaccgcg acgcggtgct cggctgggac
     6061 ccggcgcatt ccgacccgcg cgcgacgtgg cgggagaccg acgtctacta cggcgagctg
     6121 gccaccgcct gcgaaccgtt cctggtggag ctggggcgca ggctggagaa ggtgcgcgcg
     6181 gagctgccgg agtcgatcgg gccggacctg gtcgacgacc tccagcgcca cctgcacggg
     6241 aggttcgcgc tggcgctggc ctgggtggtg gaggccgagg cgaacctgca ccgggcgcgg
     6301 cgcgggatcg accgggagcg ggcgggcgag gacgactacc tcgcctacct ggacgcgacg
     6361 ttcgccgatt ccgccgccta ccacgagttc ttcctggagt acccggtgct ggggcgctgg
     6421 ctggcccacc tgaccgggct gatcgccggc tacggcgcgg acctgctgcg caacctggcg
     6481 gccgacgcgg cgcggctcgg gccggagctg ttcggccggt cgatcgtcgc gttccgctcg
     6541 ctgcggctcg gcaccggcga ctaccacgcg ggcgggcgca gcgtcgcggt ggtgaccgcc
     6601 gagctcgacg acggcgggac cgggacgttc tactgcaagc cgcgctgcct gcgttccgag
     6661 gcgggggtgc aggccgtgct ggaccggttg cgcgagcggg gtgtcgtgga gttcgcctcc
     6721 cggcccgtgc tggtgcgcga cggctacggc tacgaggccg cgatcccgac cgggcgcaac
     6781 gagaccggca ccgccgaagc ggccgcgcac gtgtaccggg agctcggcgg ccacctggcc
     6841 ctgttctacg tgctcggcgg cagcgacctg cacttcgaga acatcatcgt cgccgacggc
     6901 cacgtccacg tgtgcgacgg ggagacggtg ttcgacgcgt cgccgctggg ccgcggcggc
     6961 agcggaacgg tgcaggactc ggtgttcaag acgggcctgc tggagtggcc gcgcgccgga
     7021 accgcggacg gcccggagca gatgcggctg agcgggtgca ccggcggcga gagctacgag
     7081 atcccgatgc cggtgccgca gatcgtcggc gggcgcctcg gcttcggcgc tgcggtgcgg
     7141 aaccggtcgg gcgtgcgggt ggagctcgac gccaccaacc gggtcttcct gcacggggag
     7201 ctcgtccacc cgcaggacca cctggagtcc atcgtggacg gtttcgaccg ggtgcacgag
     7261 tggctggagc gcaacgccga cgaggcggtc gccctggttt cggaagcgtt ccgcggcgcc
     7321 tccgcccggt tcctgaactg gagcacccag atctacgcgc agctgatcgc gtccgcgcgg
     7381 cacccgaagt gcctggccga cccgctggag gtcgacctgc tgctgaacac ggtgcgcacc
     7441 ttcccccgcg aatgggaccg cgacgccgtg ctctcggcgc gggagacggc gtcgatgtgg
     7501 cggctggacg tgccggtctt caccgtcgag gtgggcgacg accacctcgt gcacgaccgc
     7561 gcggaacggc tgccgaccgc gctggacggt tccccgctgg agcgcgccgc ggagcggatc
     7621 cgctcgctca ccgcgaagaa ccgcgcgcag caggcccgct acatcgccgc cagcctgtcg
     7681 aacgacgaga tcagcagccc cgcgttcgtg gccaccgcgc tggactacgc ggtgcgggtc
     7741 ggcgagcggt tgtgctcgat ggagctggaa ccgccggctc cggcgccgtg gaagtcgttc
     7801 ggtcgggtcg acggcgtgct ggagtccgtc gacatcgacg cggacctcta caccggcacc
     7861 gcgggggtgg cgctgttcct cgcctacctg gacgacgtgg tgccgcgccc cgagttccgg
     7921 cgggccgcgc ggcgcgcgct gcggcacgtc ctcgacaccc cgccgcagcg caccggagcg
     7981 ttcgccgacg tcggcgggcg gctgtacctg ctcacccacc tgcaccggtt gtggggcgag
     8041 caggaactgg tggacgaggc cgtgcgggcg tgcgacggcc tggccgagct catcgacgcc
     8101 gacacccacc tggacctgct gcacggggcc gccgggatga tcccggtgct gctggcgctg
     8161 gagccgctgc tcggggatca ggcgctggac ctggcgcacc gctgcgcgga ccacctgctg
     8221 cggcgcgccc gcgatcggga cggcgcggtg agctggccgt ccaacacccc ggaacacgcc
     8281 cgggacgaca tgaccgggtt cgcgcacggc gccgccggga tcggctgggc gctggtcctg
     8341 ctgggcaccc gcaccggccg ggacgagtgc gtcgcggcgg gcaggagcgc cttcgcctac
     8401 gaggcgcggc acttcgacct cgacgagcag gactggtacg acctgcgcta cgacacgggt
     8461 caggtcgcga agaacggcca ccactactcc aacgcgtggt gcaacggggc cgccgggatc
     8521 gggttgagcc gcatcgccgc ctggtcgttg ctgggcgcgg acgacgagga gctgctgcgg
     8581 gaggcgcggc acgcgctggg ggcgaccgtg cgcaacttct cccggctgaa gaaccacacg
     8641 ctgtgccacg gcacctccgg caactcggag ctgctgctgc gctacgcgct gctgcgcgac
     8701 gaacccgcgt tccggctgga ggcgaacgtg caggtgcgcg agatgtggcg ctcgctggac
     8761 gacgccgagc acggcctcgg cgagcagagc gccgacttct tccccgggct gatgatgggg
     8821 atctccggct acggcatgca cttcctgcgg ctcgcgcacc cggaacgggt gccctcggtg
     8881 ctgatgctcg acccgccgcg accgtgagca ccgccctccg cttccccgaa cctcccgccc
     8941 caccaccgaa tcccggcatc ccagcgaccc ggcgggcagc gacccgccac ccgcaccgga
     9001 cgaaggagac cgccatgacc gtcgaagcct gcaagagctt cctgcgcgcg accaggcagc
     9061 agccggaact cggccagcag ctcaaggccg tcaccgggat ggaggagatg atcgcgctcg
     9121 gcggcaggca cgggttcgac ttcaccgccc aggaacttgc cgcgggatcc gccgagctcg
     9181 gcgcggagac cacgcccgaa ccggccgccg acgaaccggc gcccccgcgg ggaacggcct
     9241 tctaccacca cgagtacgac ctggcggacg tgccggagct ggcgccgctg atcgacgagc
     9301 tgccgctact gaaggtccgt cccccggtgg acctggcggc gttccgcgcc cggttccgcg
     9361 ccgacgacct ggaatcgacc tcgaagtccc cgtccgatcc cgagttccag gagtggaacc
     9421 gccggatgat ggccgcgcac tggcaggacc cggccggcgg tggcgcgcgg cgggacttcc
     9481 acctggtgaa cctggacgag cacgtcgagc accccggcta ccccgcgtac ttcgccgcca
     9541 agacgcgggc gatccggttg ctggaaggcg tgttcggcga cgagatccgg ttgtccggct
     9601 cgatgtggta cccgccgaac agctaccggc tgtggcacac caacgagaac cagccgggct
     9661 ggcggatgta cgtggtggac ctcgacgacg acttccccga aggcggcggc acctccttct
     9721 tccgctacca gaacccgggc accggcgaga tcgtgacgct gccggagcgc cggaggatcg
     9781 tgcggttctt caaggccgag caggacccgg cgaggttgtt ctggcactgc atcgtgaacc
     9841 cgaccgaacg gcaccgctgg agcttcggct tcgtggtgcc ggaggactgg caggaccacc
     9901 tgggcagcct gcgcgccgcg tgacgggatc gccgcgcacc ccggccgttc gacagcagcg
     9961 aggagcaccc gtgcgacgac tccggcgagg cgacgaccac caccgccccg aggacgcacc
    10021 cgcgatcgtg gcccgcgacg tgcacaagtc ctaccgcggg acgcacgcgg tgcgcggcgt
    10081 cgacatcacc gtggcccgcg gcgagacgtt cggcttcctc gggccgaacg gcgcgggcaa
    10141 gtccaccacg atcgcgatga tgtgcacctt ggcgctgccc gatgcggggc gcatcgagat
    10201 cgccgggtac gacgtggtgc gcgacgcgca ccaggtgcgg cgcaggctcg gcctggtgtt
    10261 ccaggaatcg acgctcgacc cggacctgac caccgcggag aacctgggtt tccacgccga
    10321 cctgtacgcg atgccccggg cgctggtcgc cgagcgcgtc gactccatgc tcgacctggt
    10381 cgggttgcgg gagcggcgcg cggaccgggt cgcgacgttc tccggcggga tgcggcggcg
    10441 gctggagatc gcgcgcggcc tgctgcaccg gccgagcgtg ctgttcctcg acgagccgac
    10501 cgtgggcctc gatccgcaga cccgcgcgga gatctggacc tacctgcacc ggatcgccga
    10561 gcaggaggag accacggtct tcctcaccac gcactacctc gacgaggcgg agcggtgcga
    10621 ccggatcgcg atcatcgacg cgggccggat cgtcgcgcag ggcaccccgg cggagctcaa
    10681 gtcggtgatc ggcgcggacc gcatcgcgct gcgcaccggc gacgacgacg cggccgtgcg
    10741 ggtgctgcgc gagcgcttcg acctggaggc caccaccgcg gacggcggcg tgcaggtgca
    10801 ggtgtccgac ggggcgcggt tcgtgccggt gctctgctcc ggcctgaacg tgccgatctt
    10861 cgaggtgacg gtgacccggc cgagcctgga cgaggtgttc ctgcactaca ccggccgcgc
    10921 catccgggac gtccccggcg tgcccgcccc ctgatcccca cggccgttga ggagaccgcg
    10981 tgaccagcac cgcaccgccc cggaccgcag caccgtccga acaggacgcc gaacggagcg
    11041 gccggctcgc gcgggagtgg cgggccggga gcatggtgtg gcgccgggaa ctgctgcact
    11101 tcctgcggga ccgcacccgg gtcgcggtgt cgctgctgcc gccgttcctg ttcctgtacg
    11161 tgctcggcat cggcctgtcc cggctgctgg ccgacggggc gggcggggcg cggacggcgg
    11221 aggactacct ggtgttcctg ttccccggtg tgctggtgat ggcggcgcag gcgccggcga
    11281 tctcggtggg ggcgtcgatc gtgtgggacc ggcagaccgg gttcatgcgg gagatgctgg
    11341 cggcgccggt gttccgcggc acgctgctgc tcggcaagtg cctcggcggt gcgacggtgg
    11401 cgacggcgca gagcgcggtc gtgctgctca ccgccgggct ggtcggagtg ccctacgacc
    11461 cggtgctgtt cgcggtgctg ctggccgaga tcgcgatcgc cgcgctgtcg ctgacggtgc
    11521 tcgccgccgg ggtggccgtc gcgatcaagc gggtgcagac gttcaacacc gtgctgtccg
    11581 tgctgatcac accgctggtg ttcctgtccg ggatgatgtt cccgatcagc gcgatgcccg
    11641 ggtggatggt ggcgttgacg gtggtgaacc cgctgaccta cgcggtggac gcgatgcgcc
    11701 acgccgtcct cgggcggctc gggcaggagc actcctcggt gatcttctcg ccggtggagt
    11761 gggcgggctg ggaagtcccg ccaccggtgg agatcgccgt ggtgctcggg ttctcggtgc
    11821 tggcgctggt cgccatcagc cgccggttcg cccgcaccgg ctgaccggag gttgacgtcc
    11881 gcgcccgccg tcggcttggg tgaccgggga ccgacgtcga gcggaggggg ccgcg
//
