LOCUS       BGC0002347             15458 bp    DNA     linear   BCT 09-JUN-2020
DEFINITION  Actinoalloteichus caeruleus strain WH1-2216-6 cyanogramide
            biosynthetic gene cluster, complete sequence.
ACCESSION   BGC0002347
VERSION     BGC0002347
KEYWORDS    .
SOURCE      Actinoalloteichus caeruleus
  ORGANISM  Actinoalloteichus caeruleus
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Actinoalloteichus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:22:34
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..15458
                     /db_xref="taxon:2893586"
                     /mol_type="genomic DNA"
                     /organism="Actinoalloteichus caeruleus"
                     /strain="WH1-2216-6"
     misc_feature    1..15458
                     /note="cyanogramide biosynthetic gene cluster"
     subregion       1..15458
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002347"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..15458
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             393..1946
                     /NRPS_PKS="Domain: AMP-binding (27-417). E-value: 3.8e-76.
                     Score: 248.1. Matches aSDomain:
                     nrpspksdomains_QIC03949.1_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     298.7; E-value: 1.1e-90)"
                     /gene_kind="biosynthetic-additional"
                     /note="fatty acid CoA ligase"
                     /product="CyaA"
                     /protein_id="QIC03949.1"
                     /sec_met_domain="AMP-binding (E-value: 4.8e-81, bitscore:
                     261.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWRNPIPPEPTSEELVDYVRRVLAGIAPQDDVLVSENRSLNGAET
                     HRLVAGMAAVLARFGVGPGVRVACLHGNTPEAVLLRLAVQWAGGCYVGLRPMFSPTVHA
                     ACLAHAAPEVLVHDQQREERATELLRRASVPRVLSLGPSALGEDLAALAAAAVADAPTL
                     PAALDRPASLAYTSGTTGSPKGVVHGTTAMAACLDVARAMYGPPPWRFLVAIPLSDLGG
                     ELAQWILACGGVVVLREDLDPLATLALLERERITHLFTAPSSLYQLAEHPDLDHFDLTA
                     LRLAVYGGAPAVPARTSAALRRLGPRLMQNYGTQETGFVCALLPEDHGHPELLDHAGRL
                     LPGVEVEIRDTSGAVVPPGVVGEIWVRSPMTMSGYHADPARTGEVLVDGWVRTGDLGHL
                     SEADLLRILDRAKDLIIVEAYNVYSRRVEDVLCHHPKVLQAGVVGLSDVDRGERVCAAV
                     VLASGTADADELREHVEAQLGEPHVPRLIRFVDTIPLTDGGKPDKSALRALFAPDTPSH
                     "
     misc_feature    459..461
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     aSDomain        474..1643
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_QIC03949.1_AMP-binding.1"
                     /evalue="3.80E-76"
                     /label="QIC03949.1_AMP-binding.1"
                     /locus_tag="QIC03949.1"
                     /protein_end="417"
                     /protein_start="27"
                     /score="248.1"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="PQDDVLVSENRSLNGAETHRLVAGMAAVLARFGVGPGVRVACLHG
                     NTPEAVLLRLAVQWAGGCYVGLRPMFSPTVHAACLAHAAPEVLVHDQQREERATELLRR
                     ASVPRVLSLGPSALGEDLAALAAAAVADAPTLPAALDRPASLAYTSGTTGSPKGVVHGT
                     TAMAACLDVARAMYGPPPWRFLVAIPLSDLGGELAQWILACGGVVVLREDLDPLATLAL
                     LERERITHLFTAPSSLYQLAEHPDLDHFDLTALRLAVYGGAPAVPARTSAALRRLGPRL
                     MQNYGTQETGFVCALLPEDHGHPELLDHAGRLLPGVEVEIRDTSGAVVPPGVVGEIWVR
                     SPMTMSGYHADPARTGEVLVDGWVRTGDLGHLSEADLLRILDRAKDLIIV"
     CDS_motif       906..953
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_QIC03949.1_0001"
                     /evalue="5.30E-06"
                     /label="NRPS-A_a3"
                     /locus_tag="QIC03949.1"
                     /protein_end="187"
                     /protein_start="171"
                     /score="19.1"
                     /tool="antismash"
                     /translation="SLAYTSGTTGSPKGVV"
     CDS_motif       1461..1544
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_QIC03949.1_0002"
                     /evalue="4.10E-08"
                     /label="NRPS-A_a6"
                     /locus_tag="QIC03949.1"
                     /protein_end="384"
                     /protein_start="356"
                     /score="25.6"
                     /tool="antismash"
                     /translation="PPGVVGEIWVRSPMTMSGYHADPARTGE"
     misc_feature    1908..1910
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS             complement(2019..3218)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1237:transposase
                     (Score: 429.6; E-value: 2.1e-130)"
                     /gene_kind="other"
                     /note="IS110 family transposase"
                     /product="Orf1"
                     /protein_id="QIC03950.1"
                     /transl_table=11
                     /translation="MPELWAGVDAGKAAHHCTVIDTDGTKVLSRRVANNEADLLELIGD
                     VRALAPDVLWAIDLNSGGAALLIALLVNHGQKLLYIPGRTVHRAAGTYRGDGKSDSKDA
                     FVIADQARMRRDLHPVTAGDEIAVELRILTARRYDLAADRTRAINRLRAQLLEYFPALE
                     RAFDYSTSKAALVLLTGHQTPAALRERGREQLADWLRARKVRNSATVAAAAVAAAEAQF
                     TTVPGEMTAAAMVARLAKGVMALDDEIAETDALIEGRFRRHRHAVAVLSMPGVGPLLGA
                     EFIALTGGDMAAFGSADRLAGVAGLAPVPKDSGRVRGNLRRPRRYNRRLLRMFYLSAQV
                     AARCCPTSKAFYDRKRREGKAHKQAVLALARRRLNVLWALIRDNRAFQTGTPQPEAAAA
                     "
     CDS             3455..4399
                     /codon_start=1
                     /note="Pictet-Spengler cyclization"
                     /product="CyaB"
                     /protein_id="QIC03951.1"
                     /transl_table=11
                     /translation="MRQIEIEWVQPGITVTADLNEERNPRLADLLWNELLPYNSLQNHA
                     LVSGNHLYHLVPHHQLVYTRAEYKEDRTKSPDGTVFLSQLQHLAVKYGPLSEYLPAAPV
                     GHVVPEDVEALRAAGRACWEAAWSSKQVIEVRVRRKGEDVRNFVLPRTAPVDSPAVQKL
                     VEEIHDEVARVWIDPPQEIVAMHEGRIASRAGSYDQYFSTLVFLNGEVRPLGYSALNGL
                     VTMSRTTDISLTDLLRITPMLIKTPAEFLGYTGLNTLWDFTQRVLETLPGVETRDEYFA
                     LINALALYANVLNTWNLHFFPWRAGADHAYATV"
     CDS             4420..6111
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1180:Decarboxylase, pyridoxal-dependent (Score: 141.7;
                     E-value: 4.6e-43)"
                     /gene_kind="biosynthetic-additional"
                     /note="glutamate decarboxylase"
                     /product="CyaC"
                     /protein_id="QIC03952.1"
                     /transl_table=11
                     /translation="MPSREFVETLTELRHRTLHPTEHNRGQLPERLSHALRTLAVPSHP
                     DGAWLGTAPPDLGWLRDFEAVPEHMSDPDLVLRGVTAGLGAQLRAHAPGNLFNIFPVPL
                     FDAVTAATLAQLYTPNALWDLLAGGTLEIERQLVRQLADLAGWPRDDAGGTFSFGGKAG
                     LMYAVRIGLNRCLPESGRRGLAGGAAPVVVTTEHNHDSVEPVCALLGLGSEACVRVPAP
                     GRDRATTAERVLTTVADLVARGTPVACVVLSGGSIMDHTIDPVGLVAAGLSSLSGSLPY
                     RPYLHFDTAHGWPWLFFRDYDFPGNPLEIDPVTLAALREVSDLVAEAAWADSMTADFHK
                     AGFCPYPSSAFLARNAAELHTLHEDPHTAEWRPWGGNLVLHHTIEHSRGAAGILAAFAA
                     LHSVGRTGFRVHLAHVTAMNNVFRAHLPEFGYELVNPTSPGMASVFWPVAPGGPATFAE
                     LGRAEPLLVDDANRYTQALYQRLAGLDPQHEVAAPIVLGFLPAFTHGAHGHPLSALRLL
                     PNSPHHDEVTVAAIADRMATAKAEFDRSVTSRTGPLAGLRLRHVPE"
     CDS             6159..7190
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042:O-methyltransferase (Score: 193; E-value:
                     1.3e-58)"
                     /gene_kind="biosynthetic-additional"
                     /note="methyltransferase"
                     /product="CyaD"
                     /protein_id="QIC03953.1"
                     /transl_table=11
                     /translation="MTASPRPCPAQVPDSSAALRLARFFAAGCLGQALAALVRAGVVRE
                     MARDRPSVDELAHRTGTDPATLSRFLRAGEAAGVFVEDPPHHFALSDTGELLAEGPGSL
                     GGLLELANTAPVLAAWSAAEHTLRTGESAFAAVHGSSVFDHLAQAPTLAGAVGRAMGAS
                     VAAELVPASVDLPSARHVVDVGAGGARLLTALLAAQPHLVGTLVGVPTAAREQVAEELR
                     RSGVRSRCRLPDGEVLPQDGDVYLLCHVLPCLDDEDAVSLLRSLVPVLREGARLLVIGF
                     VPSARDRTPVIPALDVWMAAMCGGRQRTEHEYSALLDRAGLLLHARHGVRPDTECVLEV
                     VRR"
     CDS             complement(7226..8008)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 43; E-value: 6.1e-13)"
                     /gene_kind="biosynthetic-additional"
                     /note="methyltransferase"
                     /product="CyaE"
                     /protein_id="QIC03954.1"
                     /transl_table=11
                     /translation="MHTDRQLVADEYYGTTRILRARQRLFDFQRPRFDLHDDVASVVAT
                     APGPVIDVGCGNGLLRARLAARRPDLDLFGVDVSTGMAPDVVGDVQAFPVADGAAGAVV
                     AMHMLYHVPDIVTAVREMARALRPDGIAVATTNDSSSLTELYTLADHAFADVGGGPVPP
                     RPDARFGLHHARLLAAAFGEVAVRRRAYTVVCDELEPLLGYLDSLRPAWRHLLPADAVW
                     EDFLTAAEGRLRAVLAGGHFTDHGEAGMIIGRLPRRAG"
     CDS             8583..9311
                     /codon_start=1
                     /note="methyltransferase"
                     /product="CyaF"
                     /protein_id="QIC03955.1"
                     /transl_table=11
                     /translation="MILDSGRNNGVNWERYWRDVTSDAGTPSPPWHFGSGAEMAPYLPV
                     MLDHLAPELPLVDLGCGDGLLTDHLARHYPVVVGVDVSPAAIAAARGRARPGLSFDVLD
                     ATDVEAAARLRATVGEANVHLRGVLHAMDPADWPAALTTLATLTGRRGRVFDIEITPAF
                     SDAVEEMLGKFAAPPPGMAAVARSGLRPTELDSPSLRALYEDTGWTVVAAEELTGRSTM
                     RLPDGSYFEYPFTYLVAGRR"
     CDS             9326..10579
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 425.2; E-value:
                     3.8e-129)"
                     /gene_kind="biosynthetic-additional"
                     /note="cytochrome P450"
                     /product="CyaG"
                     /protein_id="QIC03956.1"
                     /sec_met_domain="p450 (E-value: 2.6e-26, bitscore: 81.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGGAEVEGVPRSPDPSGPVFTLNEVPALDSDPLFALFRREHPLS
                     RIRLPHGGEGWLVSRYQDVRTVMTHPAFSAAAAASDQVPRMTVVAGRPPETIAGLDPPE
                     HTRLRRLAAPTFRASRIATFRPLATRIAVDTLTELVDAGRPADFVRHFALPFPVRLICT
                     VLGVPYEDHSRFVPWSEAVLSTTGLSGAESAQALEEMKEYFRHLIAERRRAPHDDLLAD
                     LVAARDATPGPGESDQLSEEELVMFAVLLLIAGYETSINQIGASCFLLLERRSRWEQLV
                     AHPRLVTRAVEELLRFVPLINGVTLPRVAVSDVEVAGGTIRAGEAVFVSIPSANRDERI
                     FDDPDTFDPTRVHNQHVAFGHGPHYCLGAHLARLELQVALTELIRVLPSLRLATPRDDV
                     PWRTGLVARGPTALAVSW"
     CDS             10626..11843
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 442.2; E-value:
                     2.7e-134)"
                     /gene_kind="biosynthetic-additional"
                     /note="cytochrome P450"
                     /product="CyaH"
                     /protein_id="QIC03957.1"
                     /sec_met_domain="p450 (E-value: 9.1e-25, bitscore: 76.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQEDASGVCFLEEMDALEIDPHFAMARRERPLSRIKLLYGDEGW
                     LVTRYEDVKLVLSDPRFSAVAAANDNVPRMTAVGKPVDTLAGLDPPEHTRLRRLATPAF
                     TVSRLESFRPQAVRIATELLDALREAGPPGDVVQGLALPFPVLVICEMLGVPYEDRARF
                     LPWSDTILATTAHSPEEAVVALEEMKEYFRHLIAERRRAPHDDLLADLVAARDATPGPG
                     ESDQLSEDELVMFASVLLIAGHETSVNQIGDSCFLLLRDRHRWELLQRRPELLPKVVEE
                     LLRYVPLVNGVILPRVATEDVEVAGGVIRAGEAVFASTAAANRDERFFDRADELDLLRQ
                     RNPHLAFGYGPHYCLGAHLARMELRIALGELLRTFPALRLADPPEEVRWRSGLVMRGPV
                     ELRVTW"
     CDS             12226..13440
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 451.5; E-value:
                     4.1e-137)"
                     /gene_kind="biosynthetic-additional"
                     /note="cytochrome P450"
                     /product="CyaI"
                     /protein_id="QIC03958.1"
                     /sec_met_domain="p450 (E-value: 4.3e-24, bitscore: 74.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDTTTDMVFGLSEIDGIEIDPLFGRLRRERPLARIRMPFGGEGW
                     LVTRYEDVRTVLADPRFSAAAAAGDSVPRMTAVGRPDDTIAGLDPPDHSRLRRLAAPAF
                     SPARLESFRPRATRIASDLLDAVERTGPPADLVTALGLPFPVQVICEILGVPYEDRANF
                     LPWSDAVLSTTAYTAEQAAASLAEMKAYFAHLVERHRVHRHDDLLADLVAARDTTVPGE
                     ADRLSEDELVMFALVLLIAGHETTANQIGNSCFLLLSDRSRWEQLVDQPELIPRAVDEL
                     LRFVPLVNGVTLPRVAKVDVEIAGGLVRAGEAVFTSTSAANRDERVFEDPERLDLTRRH
                     NPHVAFGHGPHHCIGAQLAKVELQIALGELVRRFPTLRLAGPAEEVPWRRGLVMRGPTE
                     LRVSW"
ORIGIN
        1 ccgggctcct cctggttggt gtcggctact gcggcaggtg ggccggcagc gagtggccca
       61 ccgtgactga atgtcgaatc ctacccggtg ggggccctca acccggtagc gcccgcgtgg
      121 tgagaggcct cactgtcggg cgacgcgggt cccccggacg ggttgacggt caaccgttca
      181 ggaaaagcgg cgacccccac acgggaatac cccgagcaaa cagccggttc cgtgacccac
      241 ggtcgtgtcc gatgctcggc gagagggaac ggaagatggc gttgcgtatt ccgatttcgc
      301 ccctggcgag tattccttgg ttccatcggg tctccgtcac cgaaatatcg agtatcgctc
      361 attgcgcctc cgttcgcctt tcggaatcgt cgttgtggcg caacccgatc cccccggaac
      421 caacgtcgga ggaactggtg gactacgtgc gacgggtctt agccggaatc gccccccagg
      481 acgacgtcct cgtttccgag aaccggtcgc tgaatggcgc cgaaacccat cgtctggtcg
      541 ccggtatggc tgcggtcctc gcgcggttcg gagtgggccc cggagtccgg gtcgcctgcc
      601 tgcacgggaa cacgccggag gccgtcctcc tccggctcgc ggtccaatgg gcggggggct
      661 gctacgtcgg cctgcgcccg atgttctccc ccaccgtgca cgcggcgtgc ctcgcccacg
      721 cggcgccgga ggtgctcgtc cacgaccagc agcgcgagga gcgcgccacc gaactgctcc
      781 gccgcgcgtc ggtgccccgc gtcctctcgc tgggtccctc cgctctcggc gaggacctgg
      841 ccgcactcgc cgccgccgcc gtggccgacg ctcccacgct ccccgccgcg ctcgacaggc
      901 cggcgtcgct ggcctacacc agcgggacga ccggcagccc caagggcgtc gtccacggca
      961 ccaccgccat ggcggcgtgc ctcgacgtgg cccgcgccat gtacggaccg ccaccctggc
     1021 gcttcctggt ggccatcccg ctctccgacc tgggcggaga gctcgcccag tggatcctcg
     1081 cctgcggcgg ggtggtggtg ctgcgggagg acctggaccc cctggcgacg ctcgcgctgc
     1141 tggagcggga acgcatcacc cacctgttca ccgcgcccag ctcgctctac cagctcgcgg
     1201 agcaccccga cctcgaccac ttcgacctca cggccctgcg actcgccgtc tacggagggg
     1261 cccccgccgt gccagcccgc acgtccgccg ccctgcgccg gctcggcccc cgcctcatgc
     1321 agaactacgg cacccaggag accgggttcg tgtgcgcgct cctgcccgag gaccacgggc
     1381 accccgagct gctggaccac gccggtcggc tgctcccggg agtcgaggtg gagatccggg
     1441 acacctcggg cgcggtcgtc ccgccggggg tggtcggcga gatctgggtc cgctccccga
     1501 tgacgatgag cgggtaccac gccgatccgg cccggacggg cgaggtgctg gtggacgggt
     1561 gggtccgcac cggtgacctg ggccacctct ccgaggcgga cctgctccgc atcctcgacc
     1621 gcgccaagga cctcatcatc gtcgaggcgt acaacgtcta ctcccgtcgg gtggaggacg
     1681 tcctctgcca ccacccgaag gtcctccagg ccggggtcgt aggcctttcc gacgtcgacc
     1741 gaggtgagcg ggtctgcgcg gcggtcgtcc tcgcgagcgg aaccgcggac gccgacgagc
     1801 tccgcgaaca cgtcgaggcg caactcggtg aaccacacgt accccgcctc atccgattcg
     1861 tcgacaccat tccactcacc gacgggggaa aaccggacaa gtcggcatta cgcgccctgt
     1921 tcgcccccga caccccctcc cactgatccc acgaccaacc ggacgaaagg attcccaatg
     1981 aagttgtcaa gcggtgaagg tgtcgggccg tgagctcgtc aggccgctgc ggcttcgggt
     2041 tgcggcgtgc cggtctggaa ggcgcggttg tcgcggatga gtgcccacaa cacgttcagg
     2101 cgtcggcggg ccagcgcgag gacggcctgt ttgtgcgcct tgccttcgcg ccttttcctg
     2161 tcgtagaagg ctttcgacgt cgggcaacag cgggcggcga cctgcgcgga caggtagaac
     2221 atgcgcagca ggcgccggtt gtagcggcgt ggccggcgca ggttgccccg gactcgtccg
     2281 gagtccttgg gcaccggcgc caggcccgcg acaccggcca gccggtcggc gctgccgaac
     2341 gcggccatgt cgccgccggt cagcgcgatg aactcggctc cgagcagggg cccgacgccg
     2401 ggcatgctca ggaccgcgac ggcgtgacgg tgtcggcgaa accggccctc gatgagcgcg
     2461 tcggtctccg cgatctcgtc atcgagggcc atcaccccct tggcgagccg ggccaccatg
     2521 gccgcggccg tcatctctcc ggggacggtg gtgaactggg cttcggcggc ggcgactgcg
     2581 gcggcggcga ccgtcgcgct gttgcggacc ttccgggcgc gcagccagtc ggcgagctgt
     2641 tcccggccgc gctcacgcag tgcggcgggg gtctggtggc cggtgagcag gaccagggcg
     2701 gccttggagg tgctgtagtc gaaggcccgt tccaacgcgg ggaagtactc cagcagttgg
     2761 gcgcgcagcc ggttgatcgc ccgggtgcgg tcggcggcca ggtcgtagcg gcgggcggtg
     2821 aggatccgca actccaccgc gatctcgtcg ccggcggtca ccgggtgcag gtcacggcgc
     2881 atccgggcct ggtcggcgat gacgaaggcg tccttggagt cgctcttgcc gtccccacga
     2941 taggtgccgg cggcgcggtg gacggtgcga ccggggatgt agagcagctt ctgtccgtgg
     3001 ttgaccagca atgcgatcag cagcgcggcc ccgccggagt tcaggtcgat cgcccacagc
     3061 acgtccgggg ccaaagcccg tacgtcaccg atcagctcca acaggtcggc ctcgttgttg
     3121 gcgacccggc gggaaagcac cttcgtgccg tcggtatcaa tcaccgtgca gtggtgcgcg
     3181 gccttgcccg cgtccacgcc ggcccagagt tcgggcacgg ccacctccgt cgtcgttcgt
     3241 gcggaacacc cggcagacga cctcgccggc acgtccttat gcagcgatcg gttcgctcat
     3301 cccaatcagc ggtcgagtcg tcgcgggacc tcgggcggcc aatcctttca agccacacca
     3361 acggcgacca catgacagcc acacccgaag tccccgggcc ctcccgatct tacgactgac
     3421 cagaacagac ccgacttaga agataaggaa cagcatgcgg cagatcgaga tcgaatgggt
     3481 gcagcccgga atcaccgtca ccgccgacct gaacgaggaa cgcaatccgc ggctggcgga
     3541 tctgctgtgg aacgaactgc tcccgtacaa cagcctccag aatcacgcac tggtatccgg
     3601 gaaccacctc taccacctcg ttccccacca ccagctcgtc tacacccgcg ccgagtacaa
     3661 ggaggaccgg acgaagtccc cggacggaac ggtgttcctc tcccagctgc aacacctcgc
     3721 cgtcaagtac gggccgctca gcgagtacct ccccgccgcc ccggtcgggc acgtggtacc
     3781 ggaggacgtc gaggccttgc gcgccgccgg ccgcgcctgc tgggaggcgg cgtggagcag
     3841 caagcaggtg atcgaggtcc gggtccggcg caagggggag gacgtgcgga acttcgtgct
     3901 cccgcgcacc gccccggtgg actcccccgc cgtgcagaaa ctggtggagg agatccacga
     3961 cgaggtcgcg cgggtgtgga tcgatccacc ccaggagatc gtcgccatgc acgaggggcg
     4021 gatcgccagc cgcgccggta gttacgacca gtacttctcc acgctcgtct tcctcaacgg
     4081 cgaggtgcgg ccgctgggct actccgccct caacggcctg gtgacgatgt cccgcaccac
     4141 cgacatctcc ctcaccgacc tcctccgcat cacaccgatg ctgatcaaga cgcccgccga
     4201 gttcctcggc tacaccgggc tcaacacctt gtgggacttc acccaacgcg tcctggagac
     4261 gctgcccggc gtggagacgc gtgacgagta cttcgccctg atcaacgcgc tcgcgctgta
     4321 cgccaacgtc ctgaacacct ggaacctgca cttctttccc tggcgagccg gcgccgacca
     4381 cgcctacgcg accgtctgat ccggtcgaag gagtcagcca tgcccagcag ggaattcgtc
     4441 gagacgctga ccgaactgcg tcaccgcacg ctgcatccca cggagcacaa ccgaggtcag
     4501 cttcccgaac ggctcagcca cgcgctccgc acgctggccg tgccctccca tcccgacggt
     4561 gcctggctag gcacggctcc tcccgacctc ggctggctgc gggacttcga ggcggtgccc
     4621 gagcacatgt ccgaccccga cctggtgttg cgcggggtga ccgccgggct gggcgcgcaa
     4681 ctgcgcgccc acgcccccgg caacctgttc aacatcttcc cggtcccgct gttcgacgcg
     4741 gtgaccgcgg ccacgctggc ccagctctac acccccaacg cgctgtggga cctcctggcc
     4801 ggtggcacgc tcgagatcga acgccagctg gtccgacagc tcgccgacct ggctggctgg
     4861 ccacgcgacg acgcgggcgg caccttctcc ttcggcggca aggccggtct gatgtacgcc
     4921 gtccggatcg ggctgaaccg gtgcctgccc gagtccggcc ggcgcgggct ggccggtggg
     4981 gccgcgccgg tggtggtcac gaccgagcac aaccacgaca gcgtggagcc ggtgtgcgct
     5041 ctcctcgggc tgggttcgga ggcgtgcgtg cgcgtgccgg caccgggacg ggaccgggcg
     5101 accaccgccg agcgggtcct caccacggtg gccgacctgg tcgcacgggg aactccggtg
     5161 gcctgcgtgg tgctctccgg cgggagcatc atggaccaca ccatcgatcc ggtcggcctg
     5221 gtcgccgccg gcctgagcag tctgagcggt tcgctgccct accggcctta cctgcacttc
     5281 gacaccgcgc acggctggcc gtggttgttc ttccgcgact acgacttccc cggcaacccg
     5341 ttggagatcg acccggtgac gctcgccgcc ctccgcgagg tcagcgacct cgtcgcggag
     5401 gcggcctggg cggactcgat gacggccgac ttccacaagg ccggcttctg cccctacccc
     5461 tccagtgcct tcctggccag gaacgccgcc gagctgcaca ccctgcacga ggacccgcac
     5521 accgccgagt ggcggccgtg gggcggcaac ctggtactgc accacaccat cgagcactcc
     5581 cggggcgcgg cgggaatcct cgcggccttc gccgcgctcc acagcgtggg gaggaccggg
     5641 ttccgggtcc acctcgccca cgtcaccgcg atgaacaacg tgttccgcgc gcacctcccg
     5701 gagttcggct acgaactggt gaaccccacg agccccggga tggcgtcggt gttctggccg
     5761 gtggcgccgg ggggcccggc caccttcgcg gaactgggcc gggcggaacc cctgctcgtc
     5821 gacgacgcca accgttacac gcaggccctc taccagcgcc tggctgggct ggacccccag
     5881 cacgaggtcg ccgcgccgat cgtgctggga ttcctgcccg cgttcacgca cggcgcgcac
     5941 ggccatccgc tgtcggcgct gcgcctgctc cccaactcgc cccaccacga cgaggtgacc
     6001 gtcgcggcga tcgcggaccg gatggccacc gccaaggcgg agttcgaccg ctccgtcacc
     6061 tcccgcaccg gtcccctcgc cggcctccgc ctccgccacg tcccggagtg agccggcacc
     6121 cgccggccct ccaggaccga caccccgagg agttcgaggt gaccgcatcc ccccgcccgt
     6181 gcccagccca ggttcccgac tcgtccgcgg cgttgcggct cgcccggttc ttcgccgcgg
     6241 gatgcctggg acaggcactc gccgctctgg tcagggcggg cgtcgtccgg gagatggcca
     6301 gggaccggcc ctccgtcgac gaactcgccc accggaccgg gaccgatccg gcgacgctgt
     6361 cgcggttcct gcgcgccgga gaggcggctg gcgtgttcgt ggaggaccca ccacaccact
     6421 tcgccctgtc ggacacggga gaactgctgg cggaggggcc gggatcgctg ggtggtctcc
     6481 tcgaactggc gaacacggcc ccggtgttgg ccgcgtggtc cgccgccgag cacaccctgc
     6541 gcaccgggga gtccgccttc gcggcggtgc acggctcgtc cgtgttcgac cacctcgcgc
     6601 aggcgccgac cctggccggc gcggtggggc gggcgatggg ggccagcgtc gccgcggaac
     6661 tcgtcccggc ctcggtggat ctcccctcag cgcgccacgt ggtcgacgtg ggcgcggggg
     6721 gcgcgcgcct cctcacggcg ctgctcgccg cccaaccgca cctcgtgggg acgctggtcg
     6781 gcgtgcccac ggcggctcgg gagcaggtcg ccgaggagct gcggcggtcg ggggtgcgtt
     6841 cccgctgccg cctgcccgac ggcgaggtcc tcccccagga cggggacgtc tacctgttgt
     6901 gccacgtgct gccgtgcctc gacgacgagg acgcggtctc cctgctccga tcgctcgtcc
     6961 ccgtgctgcg ggagggcgcc cggctgctgg tgatcggctt cgttccctcg gctcgggacc
     7021 gcaccccggt gatccccgcg ttggacgtgt ggatggcggc gatgtgcggc ggacgtcaac
     7081 gcaccgaaca cgagtactcg gctctcctcg accgagccgg attgctgcta cacgcccggc
     7141 acggggtccg ccccgacacc gagtgcgtcc tcgaggtggt tcgccggtag tcgacgccac
     7201 cggccgggag ggcgttcgcg gacgctcacc cggcccggcg gggcaaccgc ccgatgatca
     7261 tgccggcctc gccgtggtcg gtgaagtgcc cgcccgcgag gacggctcgc agcctgccct
     7321 ccgcggcggt gaggaagtcc tcccagacag cgtcggccgg gagcaggtgt cgccacgcgg
     7381 gacgcaggga gtccaggtac ccgagcaggg gttcgagctc gtcgcagacg accgtgtacg
     7441 cgcgtcgccg taccgccacc tccccgaagg cggccgccag gaggcgcgcg tggtgcaggc
     7501 cgaaccgcgc gtccggccga ggcgggacgg gcccgccgcc gacgtcggcg aacgcgtggt
     7561 cggccagcgt gtacagctcg gtcagcgagc tcgagtcgtt ggtggtggcc accgcgatgc
     7621 cgtcgggccg cagggctcgg gccatctccc ggaccgccgt cacgatgtcg ggcacgtggt
     7681 agagcatgtg catggccacc acggcgccgg ccgcgccgtc ggcgaccggg aacgcctgga
     7741 cgtccccgac cacgtcgggc gccatgccgg tggagacatc gacaccgaag aggtccaggt
     7801 cgggtctgcg ggcggcgagc cgggcgcgca gcagaccgtt cccgcacccc acgtcgatga
     7861 cgggtcccgg cgcggtggcg accacggagg cgacgtcgtc gtggaggtcg aaccgaggac
     7921 gctggaagtc gaaaaggcgt tggcgtgccc gcaggatcct ggtggtcccg tagtactcgt
     7981 ccgccaccag ctgccggtcg gtgtgcatac cggaatgcta ttccccgacg ggggctccgt
     8041 ggtctcctcg agctccgatt ccggaattcg ggcgacggaa gcgtcccgcc cggtggacgg
     8101 ctctcaccga ccgtgccgac acgacctccc gcactgctcc aaccgacgga gcggaaagcc
     8161 ccccgttcag gattatcccg aaccccacga ccgcgcggaa aacgacgcgg aacacgtcat
     8221 cccaggagtc aggaacggcc gttcacccga tcagcgaggc cgggttcacg aagcgcaccg
     8281 ggcgagcgac cacccgccca tcagccaatt caccctactc ggatggggga aacagccacc
     8341 ctgccaccca ctcgttcttg ccgacgggtc accgacgttg gtatatctct attcagcggt
     8401 tcacgtcatg gattccacca ccggcccacg actccgcatt tccgatgtcg ccattcctga
     8461 ccacccggtt cgtggacgca ccagcaccca ccacagaaaa ccgggcccgc gccacctgct
     8521 tccccgtggc ccgttccggc ggccgcgatc agcgcgcacc gttccgcacg aggagaccgc
     8581 acgtgattct ggattccggc cgaaacaacg gggtcaactg ggaacgttac tggcgggacg
     8641 tcaccagcga cgccggcact cccagcccgc cctggcactt cggttcgggt gccgagatgg
     8701 ctccctacct ccccgtcatg ctcgaccacc tcgctcccga gctccccctg gtcgacctcg
     8761 ggtgcggcga cggtctcctg accgaccacc tcgccaggca ctacccggtg gtcgtgggcg
     8821 tggacgtctc cccggcggcg atcgccgccg cgcgcggccg ggcccgtccc ggtctgtcgt
     8881 tcgacgtcct cgacgccacc gacgtggagg ccgcggcgag gttgcgcgcc acggtcggtg
     8941 aggccaacgt ccacctgcgc ggggtgctgc acgcgatgga cccggccgac tggcccgccg
     9001 ccctgacgac actggcgacg ctcaccggcc gacgggggcg ggtcttcgac atcgagatca
     9061 ccccggcctt ctccgacgcc gtggaggaga tgctcgggaa gttcgccgcc ccgccaccgg
     9121 ggatggccgc ggtcgcccgc agcggcctgc ggccgacgga gctggactcg ccgagcctgc
     9181 gagccctgta cgaggacacc ggctggacgg tggtggccgc cgaggaactg acgggacgtt
     9241 ccaccatgcg gctgcccgac ggctcctact tcgagtaccc gttcacctac ctcgtggccg
     9301 ggcgacggtg agctcggagg gctccgtggc gggcggagcc gaggtggagg gtgtcccgcg
     9361 ctcaccggac ccgtccggtc cggtcttcac cctcaacgag gtcccggccc tcgacagcga
     9421 tccgctgttc gcgctgttcc ggcgggagca cccgctgagc cggatccggc tcccccacgg
     9481 cggggagggc tggctggtca gccgttacca ggacgtgcgc accgtgatga cccacccggc
     9541 gttcagcgcg gccgccgccg ccagcgacca ggtaccccgg atgacggtgg tggcgggccg
     9601 gcccccggag accatcgccg gcctcgaccc accggaacac acccggctcc gccggctcgc
     9661 cgcgcccacg ttccgggcga gccgcatcgc gaccttccgg ccgctggcca cccgtatcgc
     9721 cgtggacacc ctgaccgagt tggtggacgc cgggcgaccc gccgacttcg tacggcactt
     9781 cgccctcccg ttccccgtcc ggttgatctg cacggtcctc ggcgttccct acgaggacca
     9841 ctcccgcttc gtgccctggt cggaggccgt gctctccacg acggggctca gcggcgccga
     9901 gtcagcccag gcgttggagg agatgaagga gtacttccgc cacctcatcg ccgaacgccg
     9961 acgcgccccg cacgacgacc tgctcgccga cctcgtcgcc gcccgcgacg ccacacccgg
    10021 ccccggggag agcgatcagc tcagcgagga ggagctggtg atgttcgcgg tcctgctgct
    10081 catcgccggt tacgagacct cgatcaacca gatcggcgct tcctgcttcc tgctgttgga
    10141 acggcgttcc cggtgggagc agctggtggc gcacccgcga ctcgtcaccc gggccgtgga
    10201 ggagttgctg cggttcgtgc cgctgatcaa cggggtgacg ctgcccaggg tggcggtcag
    10261 cgacgtcgag gtcgcgggcg gcacgatccg ggccggtgag gcggtgttcg tctccatccc
    10321 ctcggcgaac cgcgacgagc ggatcttcga cgaccccgac accttcgacc cgacccgggt
    10381 ccacaaccag cacgtggcct tcgggcacgg gccccactac tgcctcgggg cgcacctcgc
    10441 ccgcctcgaa ctccaggtgg cactgaccga gctgatccgg gtgctcccct cgctgcggct
    10501 ggccacgccc agggacgacg tgccctggcg caccggtctg gtggcgcgcg ggccgaccgc
    10561 gctcgccgtc tcgtggtgag caccgcgacg ttcctccgca tccgttccga ccaggagacg
    10621 agacgatgag tcaggaagac gcgtcggggg tgtgcttcct cgaggagatg gacgccctcg
    10681 agatcgaccc ccacttcgcg atggcccgac gtgaacggcc gctgagccgg atcaaactgc
    10741 tctacgggga cgagggctgg ttggtcaccc ggtacgagga cgtgaagctg gtgctgtcgg
    10801 acccccgctt cagcgcggtg gcggcggcca acgacaacgt tccccgcatg acggcggtgg
    10861 ggaaaccggt cgacaccctc gctgggctcg atccgcccga gcacacgcgg ttgcgccggc
    10921 tcgccacccc ggccttcacc gtgtcccgcc tcgagtcctt ccgcccccag gcagtgcgga
    10981 tcgccaccga gctgctcgac gcgctgcgcg aggccgggcc acccggagac gtcgtgcagg
    11041 ggttggcgct cccgttcccg gtgctggtca tctgcgagat gctcggcgtc ccctacgagg
    11101 accgcgcgcg gttcctgccc tggtccgaca cgatcctggc cacgacggcg cactcccccg
    11161 aggaggccgt cgtggcgttg gaggagatga aggagtactt ccgccacctc atcgccgaac
    11221 gccgacgcgc cccgcacgac gacctgctcg ccgacctcgt cgccgcccgc gacgccacac
    11281 ccggccccgg ggagagcgat cagctcagcg aggacgaact ggtgatgttc gcctcggtgc
    11341 tgctgatcgc cggtcacgag acgtccgtga accagatcgg ggactcctgc ttcctcctgc
    11401 tgcgggaccg ccaccggtgg gagctcctcc agcggcgtcc cgaactgctc cccaaggtcg
    11461 tggaggagct gctccgctac gtgccgctcg tcaacggggt gatcctcccc cgcgtcgcga
    11521 cggaggacgt cgaggtggcg gggggtgtca tccgggcggg cgaggcggtg ttcgcctcga
    11581 cggcggcggc caaccgggac gagcggttct tcgacagggc cgatgagctc gacctgctcc
    11641 gccagcgcaa tccgcacctg gccttcggct acggaccgca ctactgcctt ggcgcgcacc
    11701 tcgccaggat ggaactgcgg atcgccctgg gcgaactgct gcgcacgttc cccgcgctgc
    11761 ggttggcgga cccgcccgag gaggtcaggt ggcggagtgg gttggtcatg cgggggccgg
    11821 tcgagctgcg cgtcacgtgg tgataccgcg ggcggccggg acggtgggcc aggaccctgc
    11881 cgcgatgctc gcgcccccgc cccggatcca ccccgggcgc tggtcgggcc ggtggtcccg
    11941 ggtgtcccgc cgcccgaggc gccccgcccc gcccctggcg gggaggtgcg ccaggcccgg
    12001 gcacgacctg cggtccgcgc ctcgtggcag gcgggcgagg cggcgggccg cgcggcccac
    12061 gggcgaccgc gaggcgtgct ggaccggatc ggtcccgccc ctggtccacc gggggtgccc
    12121 cagcccggac gtcccgggga gccgcgttgg agcggacctc cgatccgacg gcgcacccgc
    12181 gagtggcggg cgcacggacc ctgttgatcg acgaacggac gagagatgac ggacacgacc
    12241 acggacatgg tcttcggcct gtccgagatc gacgggatcg agatcgaccc gctcttcggg
    12301 cggctccgcc gcgaacgacc gctcgccagg atccgcatgc ccttcggcgg tgagggctgg
    12361 ttggtcaccc gctacgagga cgtgcgcacc gtcctggccg acccccggtt cagcgcggcg
    12421 gccgccgccg gcgactcggt tccccggatg accgccgtgg gcaggccgga cgacacgatc
    12481 gccggcctgg acccaccgga ccacagtcgg ctccgccggt tggcggcacc ggcgttcagc
    12541 cccgcccgcc tggagtcgtt ccgcccccgg gccaccagga tcgcctcgga cctcctggac
    12601 gcggtggagc gcacggggcc gcccgccgac ctcgtcacgg cgctgggcct cccgttcccg
    12661 gtgcaggtga tctgcgagat cctcggggtt ccctacgagg accgggcgaa cttcctcccg
    12721 tggtcggacg ccgtcctctc gaccaccgcg tacaccgccg agcaggcggc ggcgtcgctc
    12781 gcggagatga aggcgtactt cgcccacctg gtggaacggc atcgcgtcca ccgccacgac
    12841 gatctcctgg cggatctggt ggcggccagg gacaccaccg tgccgggcga ggcggaccgg
    12901 ctctccgagg acgaactggt catgttcgcg ctcgtcctgc tgatcgcggg ccacgagacg
    12961 acggccaacc agataggcaa ctcctgcttc ctgttgctct cggaccgctc gcgctgggaa
    13021 cagctggtcg accagcccga actcatcccc cgggcggtgg acgaactgtt gcggttcgtg
    13081 ccactggtca acggcgtcac gctgcccagg gtggcgaagg tggacgtgga gatcgcgggc
    13141 gggctggtga gggcaggaga ggcggtgttc acctcgacgt cggcggcgaa ccgggacgag
    13201 cgggtgttcg aggacccgga gcgcctggac ctgactcggc ggcacaaccc gcacgtcgcg
    13261 ttcggccacg ggccccacca ctgcatcggc gcgcagctgg cgaaggtgga gctgcagatc
    13321 gccctcggcg aactcgtcag acggttcccg accctgcggc tggccggccc ggccgaggag
    13381 gtgccgtggc ggcgggggct ggtgatgcgg gggccgaccg agctgcgggt gtcgtggtga
    13441 ggccgggcca cgcgcccggt cgccacgtcc ccgcgacgcg agccggtccg cctcccggga
    13501 tgcgggccgc caccgccgcg ccgcgtacct cctcccgacg tcctgacctt ggccagcgcc
    13561 gccgggtcac gatccgagcg gccggagcgt ggctcaccgc gtcccggcgc tccgccgtcg
    13621 agctggagca ccgtgaacag cggttccgcg tcggccgccg gggccgggcg gtggctggtc
    13681 ccggcaacgg ggacccgttg cgtcccggtg agcgcggttg gctaaagtaa gggcagcaac
    13741 gcccccgtag cccaatcggc agaggcagcg gactcaaaat ccgtccagtg tgcgttcgag
    13801 tcgcaccggg ggcactcgcc tttcgccggg cgatacggcc cctgaccagc atgttctggt
    13861 caggggccgc ttggcgtcgg cggtaggcgg tgtccggtgg cggcacctgt tcggcagtcg
    13921 accgcgtgct cgagcagccg ctccgccggc cgtcgtcggg cggttcggca gcccatgctg
    13981 gtacctccgc gctcctcccg ggaagccctg ccgcgcgcac ggactcgccc gtcgatctcg
    14041 gccaagcgag gccggtctcc tcggcccgct cgacgcggcg gatccgagtg cgagccagac
    14101 gacgccaggg tgtcctcgtg acgttcggga acggtgctcg cctggggcgg cggggtcagg
    14161 tgtcgcaggc ggtctcccgt tcggccgcca gggcgcggag ggccgcgcgg tcggtcttgc
    14221 cggtgccggc caccgggaac gccgggaggg tgcggcacgt ggcgggtacc ttggcggctt
    14281 cgaggaaccg ccgcagttcc cgcaggacct ggtggctccc cagctccgtc acggcgaaca
    14341 ggaccgcgtc ccggccgccc gccgggggca ccaccacggc gtcggtgacc tcggggacct
    14401 gacgcgccgc cgcctcgatc tcggcggcgc tggtgcgcac gccgcgttgc ttgaagatgt
    14461 cgtcccggcg gccgtggaag tacaggtggc cgtcgctgtc gagacggccg tggtcgccgg
    14521 tgcgcaggag gcgttctccc gtgcgcgggt cccggccgaa cgtcctcgtg gtcagcgatt
    14581 cgtcacgcca gtagccggcc atgacgtgcg gcccggagac gacgatctca ccctcggtcc
    14641 cggccggcac cggccggcca gccgggtcca ggatcgacac cgacgtcccg ggtagtgggg
    14701 ttccgagcga accgggccgg accaggtcgc tgtcggcctc ggcgatggtg acccgtttgc
    14761 attcggtgat gccgtacatc agtcgcacgg cggcggtcgg gaaggtccgc cggaggtcct
    14821 cgatgcccgt cggggtcagc tcctggcccg tgttggtcag caggcgaacg ctgggcgcgg
    14881 gggcgcgccg gccgagccgg gtgagcacgg cggccagcgg gggcacgacc gggacgacgg
    14941 tgacctcgtg gcggcggatg gtcgcgagca ggccggcgtc gtcgcctggc ccgcgcagca
    15001 ccaccgccgc gcccacccgg gcggccagga gtgcctggta gaggccgtag tcgaaggaca
    15061 acggcagccg gcacaggatg acgtcgtccg ctcggtaccc gagggtgttg ccgatcgcgc
    15121 gcacggcgaa catgaccgcg cggtgggggc agacgacggc cttcggttcg gaggtgctcc
    15181 cggaggtgta gagcaggatg gcggcggtgt cggggtccgg ggcgcggggc ggcgtgggtg
    15241 gtcggcgcgg gccaccggtg gtggggggag gttcggtcag gaccagtgtc ggctccgcgt
    15301 cggaggtgat ccgctcgagt cgctggggtg gggcctccgg gttgaggagc acggcggcga
    15361 cgccggcccg ggagcacgcg tagagggcgg cgagtaccca cgcccgcgcg ctggccctga
    15421 tcgcgacccg gtccccgggg cgtgctcccg agcggacc
//
