BGC0002350: bleomycin A2 biosynthetic gene cluster from Streptomyces mobaraensis NBRC 13819 = DSM 40847
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Location: 67,932 - 158,889 nt. (total: 90,958 nt).
This entry is originally from NCBI GenBank CP072827.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0002350
Short description bleomycin A2 biosynthetic gene cluster from Streptomyces mobaraensis NBRC 13819 = DSM 40847
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: unknown
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Unknown)
  • saccharide (None)
Loci
CP072827.1
67932 - 158889
via Knock-out studies
Compounds
  • bleomycin A2
  • bleomycin B2
Species Streptomyces mobaraensis NBRC 13819 = DSM 40847 [taxonomy]
References
Chemical products information
bleomycin A2 Evidence:
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Chemical database entries
NPAtlas
bleomycin B2 Evidence:
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Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • J7W19_00305
  • QTT77479.1
 67932 - 69590 (-) ABC transporter ATP-binding protein
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    • J7W19_00310
    • QTT72078.1
    		 69743 - 70723 (-) amidinotransferase
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      • J7W19_00315
      • QTT72079.1
      • blmB
      		 70759 - 71664 (-) bleomycin family antibiotic N-acetyltransferase BlmB
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        • J7W19_00320
        • QTT72080.1
        		 71661 - 72062 (-) hypothetical protein
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          • J7W19_00325
          • QTT72081.1
          		 72059 - 72640 (-) ankyrin repeat domain-containing protein
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            • J7W19_00330
            • QTT72082.1
            		 72698 - 73066 (-) bleomycin binding protein BLMA
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              • J7W19_00335
              • QTT72083.1
              		 73134 - 74048 (-) aspartyl/asparaginyl beta-hydroxylase domain-containing protein
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                • J7W19_00340
                • QTT72084.1
                		 74074 - 75348 (-) radical SAM protein
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                  • J7W19_00345
                  • QTT72085.1
                  • rfaE2
                  		 75345 - 76841 (-) D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase
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                    • J7W19_00350
                    • QTT72086.1
                    		 76838 - 77110 (-) hypothetical protein
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                      • J7W19_00355
                      • QTT72087.1
                      		 77107 - 78744 (-) carbamoyltransferase
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                        • J7W19_00360
                        • QTT72088.1
                        		 78741 - 79913 (-) glycosyltransferase family 9 protein
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                          • J7W19_00365
                          • QTT72089.1
                          		 79985 - 80548 (-) MbtH family NRPS accessory protein
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                            • J7W19_00370
                            • QTT72090.1
                            		 80545 - 81933 (-) hypothetical protein
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                              • J7W19_00375
                              • QTT77480.1
                              		 81930 - 82862 (-) TauD/TfdA family dioxygenase
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                                • J7W19_00380
                                • QTT72091.1
                                		 82946 - 85768 (-) AMP-binding protein
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                                  • J7W19_00385
                                  • QTT72092.1
                                  		 85765 - 93663 (-) amino acid adenylation domain-containing protein
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                                    • J7W19_00390
                                    • QTT72093.1
                                    • asnB
                                    		 93656 - 95563 (-) asparagine synthase (glutamine-hydrolyzing)
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                                      • J7W19_00395
                                      • QTT72094.1
                                      		 95569 - 97083 (-) aspartyl/asparaginyl beta-hydroxylase domain-containing protein
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                                        • J7W19_00400
                                        • QTT72095.1
                                        		 97076 - 98053 (-) NAD-dependent epimerase/dehydratase family protein
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                                          • J7W19_00405
                                          • QTT72096.1
                                          		 98050 - 99987 (-) hypothetical protein
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                                            • J7W19_00410
                                            • QTT72097.1
                                            		 100190 - 108235 (-) amino acid adenylation domain-containing protein
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                                              • J7W19_00415
                                              • QTT72098.1
                                              		 108217 - 111831 (-) non-ribosomal peptide synthetase
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                                                • J7W19_00420
                                                • QTT72099.1
                                                		 111828 - 117353 (-) type I polyketide synthase
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                                                  • J7W19_00425
                                                  • QTT72100.1
                                                  		 117350 - 120550 (-) non-ribosomal peptide synthetase
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                                                    • J7W19_00430
                                                    • QTT72101.1
                                                    		 120547 - 126966 (-) non-ribosomal peptide synthetase
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                                                      • J7W19_00435
                                                      • QTT72102.1
                                                      		 127248 - 129179 (-) hypothetical protein
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                                                        • J7W19_00440
                                                        • QTT72103.1
                                                        		 129355 - 130074 (-) phosphatidylserine decarboxylase
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                                                          • J7W19_00445
                                                          		 130532 - 132281 (-) SulP family inorganic anion transporter
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                                                            • J7W19_00450
                                                            • QTT72104.1
                                                            		 132278 - 132619 (-) winged helix-turn-helix transcriptional regulator
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                                                              • J7W19_00455
                                                              • QTT72105.1
                                                              		 132782 - 133135 (+) alkylphosphonate utilization protein
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                                                                • J7W19_00460
                                                                • QTT72106.1
                                                                		 133772 - 142234 (+) amino acid adenylation domain-containing protein
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                                                                  • J7W19_00465
                                                                  • QTT72107.1
                                                                  		 142227 - 143240 (+) TauD/TfdA family dioxygenase
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                                                                    • J7W19_00470
                                                                    • QTT72108.1
                                                                    		 143251 - 144003 (+) thioesterase
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                                                                      • J7W19_00475
                                                                      • QTT72109.1
                                                                      		 144000 - 145283 (+) MFS transporter
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                                                                        • J7W19_00480
                                                                        • QTT72110.1
                                                                        		 145291 - 146109 (+) hypothetical protein
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                                                                          • J7W19_00485
                                                                          • QTT72111.1
                                                                          		 146106 - 147842 (+) non-ribosomal peptide synthetase
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                                                                            • J7W19_00490
                                                                            • QTT72112.1
                                                                            		 147839 - 148825 (+) TauD/TfdA family dioxygenase
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                                                                              • J7W19_00495
                                                                              • QTT72113.1
                                                                              		 149009 - 156982 (+) type I polyketide synthase
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                                                                                • J7W19_00500
                                                                                • QTT77481.1
                                                                                		 157074 - 157691 (-) helix-turn-helix domain-containing protein
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                                                                                  • J7W19_00505
                                                                                  • QTT72114.1
                                                                                  		 157873 - 158889 (+) alpha/beta hydrolase
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                                                                                    Biosynthesis information

                                                                                    Biosynthetic modules

                                                                                    Name
                                                                                    Unk01
                                                                                    Type
                                                                                    nrps-type1
                                                                                    Genes
                                                                                    J7W19_00430
                                                                                    Substrates
                                                                                    asparagine (evidence: Structure-based inference [1])
                                                                                    Integrated Monomers
                                                                                    Domains
                                                                                    adenylation
                                                                                    Name
                                                                                    Unk02
                                                                                    Type
                                                                                    nrps-type1
                                                                                    Genes
                                                                                    J7W19_00430
                                                                                    Substrates
                                                                                    histidine (evidence: Structure-based inference [1])
                                                                                    Integrated Monomers
                                                                                    Domains
                                                                                    adenylation
                                                                                    Name
                                                                                    Unk03
                                                                                    Type
                                                                                    nrps-type1
                                                                                    Genes
                                                                                    J7W19_00425
                                                                                    Substrates
                                                                                    alanine (evidence: Structure-based inference [1])
                                                                                    Integrated Monomers
                                                                                    Domains
                                                                                    adenylation
                                                                                    Name
                                                                                    Unk04
                                                                                    Type
                                                                                    nrps-type1
                                                                                    Genes
                                                                                    J7W19_00415
                                                                                    Substrates
                                                                                    threonine (evidence: Structure-based inference [1])
                                                                                    Integrated Monomers
                                                                                    Domains
                                                                                    adenylation
                                                                                    Name
                                                                                    Unk05
                                                                                    Type
                                                                                    nrps-type1
                                                                                    Genes
                                                                                    J7W19_00385
                                                                                    Substrates
                                                                                    beta-alanine (evidence: Structure-based inference [1])
                                                                                    Integrated Monomers
                                                                                    Domains
                                                                                    adenylation
                                                                                    Name
                                                                                    Unk06
                                                                                    Type
                                                                                    nrps-type1
                                                                                    Genes
                                                                                    J7W19_00385
                                                                                    Substrates
                                                                                    cysteine (evidence: Structure-based inference [1])
                                                                                    Integrated Monomers
                                                                                    Domains
                                                                                    adenylation
                                                                                    Name
                                                                                    Unk07
                                                                                    Type
                                                                                    nrps-type1
                                                                                    Genes
                                                                                    J7W19_00380
                                                                                    Substrates
                                                                                    cysteine (evidence: Structure-based inference [1])
                                                                                    Integrated Monomers
                                                                                    Domains
                                                                                    adenylation
                                                                                    Annotation changelog

                                                                                    Entry version: 1

                                                                                    Date
                                                                                    Changes
                                                                                    Submitters
                                                                                    Reviewers
                                                                                    2022-09-15
                                                                                    Entry added
                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                    2022-09-15
                                                                                    Added NRP substrate specificities
                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                    Detailed domain annotation
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