BGC0002367: rotihibin A biosynthetic gene cluster from Streptomyces scabiei 87.22
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Location: 331,163 - 401,309 nt. (total: 70,147 nt).
This entry is originally from NCBI GenBank FN554889.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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General
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NRPS/PKS domains
General information about the BGC

MIBiG accession | BGC0002367
Short description | rotihibin A biosynthetic gene cluster from Streptomyces scabiei 87.22
Status | Quality: questionable The quality level of this entry. Status: active The status of this entry. Completeness: unknown Whether the entry covers everything needed for the pathway producing the compound(s)

Biosynthetic class(es)

- NRPS (Type I)

Loci
FN554889.1
351163 - 384064
via Knock-out studies
Compounds

- rotihibin A

Species Streptomyces scabiei 87.22 [taxonomy]
References

- [1] Identification of Novel Rotihibin Analogues in Streptomyces scabies , Including Discovery of Its Biosynthetic Gene Cluster. Planckaert, S et al., Microbiology Spectrum (2021) DOI:10.1128/Spectrum.00571-21
- [2] Identification of Novel Rotihibin Analogues in . Planckaert S et al., Microbiol Spectr (2021) PMID:34346752

Chemical products information
rotihibin A Evidence:
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Chemical database entries
NPAtlas
List of genes involved in compound(s) production

Identifiers | Position | Product | Functions | Evidence | Extra
--- | --- | --- | --- | --- | ---
SCAB_3221 CBG67531.1 | 351163 - 353472 (-) | NRPS multienzyme (AMP-binding and pcp) |  |  | copy AA seq copy Nt seq
SCAB_3231 CBG67532.1 | 354017 - 354841 (-) | putative oxidoreductase |  |  | copy AA seq copy Nt seq
SCAB_3241 CBG67533.1 | 354889 - 355602 (-) | putative thioesterase |  |  | copy AA seq copy Nt seq
SCAB_3251 CBG67534.1 | 355599 - 356753 (-) | putative oxidoreductase |  |  | copy AA seq copy Nt seq
SCAB_3261 CBG67535.1 | 356991 - 357893 (-) | putative integral membrane transport protein |  |  | copy AA seq copy Nt seq
SCAB_3271 CBG67536.1 | 357893 - 358852 (-) | putative ABC transporter ATP-binding protein |  |  | copy AA seq copy Nt seq
SCAB_3281 CBG67537.1 | 359052 - 360749 (+) | putative NRPS-associated AMP-binding protein |  |  | copy AA seq copy Nt seq
SCAB_3291 CBG67538.1 | 360746 - 362557 (+) | putative oxidoreductase |  |  | copy AA seq copy Nt seq
SCAB_3301 CBG67539.1 | 362554 - 364266 (+) | putative oxidoreductase |  |  | copy AA seq copy Nt seq
SCAB_3311 CBG67540.1 | 364406 - 364687 (+) | putative carrier protein for NRPS multienzyme |  |  | copy AA seq copy Nt seq
SCAB_3321 CBG67541.1 | 364765 - 381309 (+) | putative non-ribosomal peptide synthetase |  |  | copy AA seq copy Nt seq
SCAB_3331 CBG67542.1 | 381487 - 381696 (+) | MbtH-like protein |  |  | copy AA seq copy Nt seq
SCAB_3341 CBG67543.1 | 381729 - 382730 (+) | putative oxidoreductase |  |  | copy AA seq copy Nt seq
SCAB_3351 CBG67544.1 | 382739 - 384064 (+) | putative aminotransferase |  |  | copy AA seq copy Nt seq

Biosynthesis information

#### Biosynthetic modules

Name
Unk01
Type
nrps-type1
Genes
SCAB_3221
Substrates
allo-threonine (evidence: Structure-based inference [2])
Integrated Monomers
Domains
adenylation
Name
Unk02
Type
nrps-type1
Genes
SCAB_3321
Substrates
citrulline (evidence: Structure-based inference [2])
Integrated Monomers
Domains
adenylation
Name
Unk03
Type
nrps-type1
Genes
SCAB_3321
Substrates
2,4-diaminobutyric acid (evidence: Structure-based inference [2])
Integrated Monomers
Domains
adenylation
Name
Unk04
Type
nrps-type1
Genes
SCAB_3321
Substrates
3-hydroxyasparagine (evidence: Structure-based inference, Homology)
Integrated Monomers
Domains
adenylation
Name
Unk05
Type
nrps-type1
Genes
SCAB_3321
Substrates
asparagine (evidence: Structure-based inference [2])
Integrated Monomers
Domains
adenylation
Annotation changelog

#### Entry version: 1

Date
Changes
Submitters
Reviewers
2022-09-15
Entry added

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2022-09-15
Added NRP substrate specificities

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

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