BGC0002430 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002430 (Streptomyces kanamyceticus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,betalactone	1	121,518	gausemycin A/gausemycin B	NRPS:Type I+saccharide	100%

BGC0002430 - Region 1 - NRPS,betalactone

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Location: 1 - 121,518 nt. (total: 121,518 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
betalactone: (HMGL-like and AMP-binding)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 121,519
Label		BGC0002430

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002430	1.36	NRP, Saccharide	gausemycin A, gausemycin B	Streptomyces kanamyceticus
BGC0001133	0.79	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002017	0.70	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0002518	0.67	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0002135	0.67	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833	0.65	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825	0.63	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0000392	0.63	NRP	mirubactin	Actinosynnema mirum DSM 43827
BGC0001945	0.63	NRP	chloromyxamide	Myxococcus sp.
BGC0001128	0.63	NRP	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002430	1.00	NRP, Saccharide	gausemycin A, gausemycin B	Streptomyces kanamyceticus
BGC0001133	0.76	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001050	0.72	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0002071	0.70	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001514	0.69	Other	aristeromycin	Streptomyces citricolor
BGC0001185	0.69	NRP	bacillibactin	Bacillus velezensis FZB42
BGC0000331	0.69	NRP	cyanopeptolin	Planktothrix agardhii NIVA-CYA 116
BGC0001561	0.69	NRP	curacomycin	Streptomyces curacoi
BGC0002017	0.69	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0001833	0.69	NRP	icosalide A, icosalide B	Burkholderia gladioli

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 4625 - 110369:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Orn - D-X - D-X - X - D-Leu) + (X) + (Asp - Gly - Ser - Gly - D-X) + (Gly - Pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

orf19

CAL_domain (46..477): pk

minowa: NH2

Prediction, score:

NH2: 118.9
fatty_acid: 31.3
AHBA: 29.1
shikimic_acid: 25.7
Acetyl-CoA: -0.2

gauA

Search NORINE for peptide: strict or relaxed

AMP-binding (468..870): X

nrpys: me-bAla

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IDTTVSLGDK
Nearest Stachelhaus code(s):: me-bAla LDTTVSLGDK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1551..1953): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DTEDLGLVDK
Nearest Stachelhaus code(s):: Orn DTEDMGLVDK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (2614..3026): X

nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DTWATNGVGK
Nearest Stachelhaus code(s):: Kyn DAWTTTGVGK (68% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (4134..4534): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DANQAGLVDK
Nearest Stachelhaus code(s):: Gln DAWQFGLVDK (59% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (5671..6064): X

nrpys: Phe, Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTTAAVCK
Nearest Stachelhaus code(s):: Phe or Tyr DASTTAAVCK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (7361..7752): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALLVGAVMK
Nearest Stachelhaus code(s):: Leu DALLVGAVAK (68% 8Å match)
Stachelhaus code match:: 90% (moderate)

gauB

AMP-binding (207..612): X

nrpys: dnmeProp, Dab

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIWQSTADDK
Nearest Stachelhaus code(s):: dnmeProp or Dab DIWQSTADDK (91% 8Å match); Asp DIWQSTADDK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

gauC

Search NORINE for peptide: strict or relaxed

AMP-binding (464..870): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGAVNK
Nearest Stachelhaus code(s):: Asp DLTKVGAVNK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1527..1920): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVVWK
Nearest Stachelhaus code(s):: Gly DILQLGVVWK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2585..2988): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHLSLVDK
Nearest Stachelhaus code(s):: Ser DVWHLSLVDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3644..4037): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVVWK
Nearest Stachelhaus code(s):: Gly DILQLGVVWK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4707..5136): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTTTGIAK
Nearest Stachelhaus code(s):: Kyn DAWTTTGVAK (71% 8Å match)
Stachelhaus code match:: 90% (moderate)

gauD

Search NORINE for peptide: strict or relaxed

AMP-binding (469..861): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DILQMGGVYK
Nearest Stachelhaus code(s):: Gly DILQMGMVWK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1522..1929): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQLAAHVVK
Nearest Stachelhaus code(s):: Pro DVQYAAHVVK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

orf48

AMP-binding (19..410): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DYYPTTMRIK
Nearest Stachelhaus code(s):: Asp DYYPTTMRVK (68% 8Å match)
Stachelhaus code match:: 90% (moderate)