BGC0002431 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002431 (Actinomadura sp)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	121,610	High	maduramicin	PKS+saccharide

BGC0002431 - Region 1 - T1PKS

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Location: 1 - 121,610 nt. (total: 121,610 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 121,611
Label		BGC0002431

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

madA2: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

madA8: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

madG4: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

madG7: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

madO1: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

madO2: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

madP1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

madP2: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: glycosyltransferase methyltransferase transferase

madG1: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

madG10: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

madG9: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

madM1: methyltransferase

smcogs: SMCOG1089: methyltransferase

madM2: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: esterase hydrolase

madE: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

madTE: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

madG2: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

madA1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

madA10: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

madA3: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

madA4: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

madA5: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

madA6: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

madA7: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

madA9: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002431.3	1.00	PKS, saccharide	maduramicin	Actinomadura sp.
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002431.3	1.00	PKS, saccharide	maduramicin	Actinomadura sp.
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 121610:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-mal) + (Me-redmal) + (ccmal - redmal) + (Me-ccmal - redmal) + (Me-ccmal - redmal) + (ohmal) + (Me-ohmal - Me-ohmal - ohmal) + (Me-ccmal) + (ccmal)
Non-colinear ordering:: madA1 + madA2 + madA3 + madA4 + madA5 + madA6 + madA7 + madA8 + madA9

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

madA7: mmal - mmal - mal

PKS_AT (576..870): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.0
Ethylmalonyl-CoA: 197.2
Methoxymalonyl-CoA: 172.0
2-Methylbutyryl-CoA: 140.1
trans-1,2-CPDA: 128.0
Propionyl-CoA: 124.8
Malonyl-CoA: 107.9
CHC-CoA: 93.0
Isobutyryl-CoA: 77.6
Acetyl-CoA: 53.5
Benzoyl-CoA: 46.5
fatty_acid: 38.5
inactive: 33.0
3-Methylbutyryl-CoA: 28.2

PKS_AT (2130..2427): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 208.1
Ethylmalonyl-CoA: 206.2
Methoxymalonyl-CoA: 174.6
2-Methylbutyryl-CoA: 148.7
trans-1,2-CPDA: 123.0
Propionyl-CoA: 108.6
Malonyl-CoA: 98.2
CHC-CoA: 97.2
Isobutyryl-CoA: 79.2
Benzoyl-CoA: 49.8
3-Methylbutyryl-CoA: 45.4
Acetyl-CoA: 44.2
fatty_acid: 38.5
inactive: 20.5

PKS_AT (3691..3993): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.7
inactive: 81.5
Methoxymalonyl-CoA: 75.5
Methylmalonyl-CoA: 71.2
Propionyl-CoA: 40.2
Isobutyryl-CoA: 35.5
Ethylmalonyl-CoA: 28.3
2-Methylbutyryl-CoA: 26.2
CHC-CoA: 24.5
trans-1,2-CPDA: 24.3
Acetyl-CoA: 23.1
fatty_acid: 22.7
Benzoyl-CoA: 15.1
3-Methylbutyryl-CoA: 8.3

madA8: mmal

PKS_AT (572..866): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.5
Ethylmalonyl-CoA: 195.4
Methoxymalonyl-CoA: 170.5
2-Methylbutyryl-CoA: 147.9
trans-1,2-CPDA: 134.2
Propionyl-CoA: 118.1
Malonyl-CoA: 106.2
CHC-CoA: 98.1
Isobutyryl-CoA: 74.8
Benzoyl-CoA: 58.9
Acetyl-CoA: 40.8
fatty_acid: 39.4
3-Methylbutyryl-CoA: 35.6
inactive: 24.8

madA9: mal

PKS_AT (574..868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.7
inactive: 78.6
Methoxymalonyl-CoA: 58.3
Methylmalonyl-CoA: 47.2
Propionyl-CoA: 32.4
Isobutyryl-CoA: 29.5
fatty_acid: 21.5
Ethylmalonyl-CoA: 19.5
Acetyl-CoA: 17.0
Benzoyl-CoA: 13.7
CHC-CoA: 12.3
trans-1,2-CPDA: 9.0
2-Methylbutyryl-CoA: 8.3
3-Methylbutyryl-CoA: 0.0

madA6: mal

PKS_AT (588..890): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 179.1
inactive: 78.2
Methoxymalonyl-CoA: 69.5
Methylmalonyl-CoA: 68.2
Isobutyryl-CoA: 50.7
Propionyl-CoA: 39.5
Ethylmalonyl-CoA: 33.0
Acetyl-CoA: 29.0
CHC-CoA: 21.0
2-Methylbutyryl-CoA: 15.8
trans-1,2-CPDA: 15.7
fatty_acid: 14.8
Benzoyl-CoA: 14.0
3-Methylbutyryl-CoA: 10.7

madA5: mmal - mal

PKS_AT (566..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.1
Ethylmalonyl-CoA: 202.0
Methoxymalonyl-CoA: 176.1
2-Methylbutyryl-CoA: 139.0
trans-1,2-CPDA: 131.7
Propionyl-CoA: 117.3
Malonyl-CoA: 105.1
CHC-CoA: 102.4
Isobutyryl-CoA: 70.8
Benzoyl-CoA: 58.5
Acetyl-CoA: 52.1
fatty_acid: 49.5
inactive: 32.7
3-Methylbutyryl-CoA: 31.0

PKS_AT (2359..2658): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 193.3
inactive: 80.4
Methoxymalonyl-CoA: 76.8
Methylmalonyl-CoA: 70.6
fatty_acid: 48.2
Propionyl-CoA: 45.5
Isobutyryl-CoA: 44.2
Ethylmalonyl-CoA: 35.2
trans-1,2-CPDA: 25.4
Acetyl-CoA: 24.4
2-Methylbutyryl-CoA: 23.1
CHC-CoA: 20.0
Benzoyl-CoA: 12.4
3-Methylbutyryl-CoA: 7.0

madA4: mmal - mal

PKS_AT (564..857): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.5
Ethylmalonyl-CoA: 203.0
Methoxymalonyl-CoA: 176.6
2-Methylbutyryl-CoA: 139.9
trans-1,2-CPDA: 131.8
Propionyl-CoA: 116.9
Malonyl-CoA: 104.5
CHC-CoA: 103.4
Isobutyryl-CoA: 71.7
Benzoyl-CoA: 56.6
Acetyl-CoA: 51.5
fatty_acid: 49.5
3-Methylbutyryl-CoA: 32.1
inactive: 30.5

PKS_AT (2339..2627): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.2
inactive: 81.7
Methoxymalonyl-CoA: 68.2
Methylmalonyl-CoA: 61.9
Isobutyryl-CoA: 39.8
fatty_acid: 39.5
Propionyl-CoA: 35.8
Acetyl-CoA: 32.1
Ethylmalonyl-CoA: 23.3
trans-1,2-CPDA: 19.3
2-Methylbutyryl-CoA: 14.5
Benzoyl-CoA: 14.1
CHC-CoA: 13.7
3-Methylbutyryl-CoA: 3.1

madA3: mal - mal

PKS_AT (583..879): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.9
inactive: 103.5
Methoxymalonyl-CoA: 61.8
Methylmalonyl-CoA: 57.4
Propionyl-CoA: 33.4
Isobutyryl-CoA: 24.1
Ethylmalonyl-CoA: 24.1
Acetyl-CoA: 12.1
Benzoyl-CoA: 11.9
2-Methylbutyryl-CoA: 7.9
CHC-CoA: 6.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2413..2714): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 168.1
inactive: 77.1
Methoxymalonyl-CoA: 72.4
Methylmalonyl-CoA: 63.0
Propionyl-CoA: 45.1
Isobutyryl-CoA: 33.6
fatty_acid: 30.3
Acetyl-CoA: 25.0
Ethylmalonyl-CoA: 22.9
CHC-CoA: 18.2
Benzoyl-CoA: 13.8
trans-1,2-CPDA: 13.5
2-Methylbutyryl-CoA: 13.4
3-Methylbutyryl-CoA: 0.0

madA2: mmal

PKS_AT (574..866): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 203.1
Ethylmalonyl-CoA: 198.3
Methoxymalonyl-CoA: 172.7
2-Methylbutyryl-CoA: 146.9
trans-1,2-CPDA: 129.8
Propionyl-CoA: 112.1
CHC-CoA: 101.0
Malonyl-CoA: 94.8
Isobutyryl-CoA: 74.7
Benzoyl-CoA: 57.2
fatty_acid: 52.3
Acetyl-CoA: 49.9
3-Methylbutyryl-CoA: 39.2
inactive: 21.8

madA1: mal - mmal

PKS_AT (520..826): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.8
inactive: 98.3
Methoxymalonyl-CoA: 72.3
Methylmalonyl-CoA: 55.6
Ethylmalonyl-CoA: 31.5
Isobutyryl-CoA: 28.6
Propionyl-CoA: 25.8
CHC-CoA: 20.6
Benzoyl-CoA: 17.3
2-Methylbutyryl-CoA: 14.6
fatty_acid: 12.7
Acetyl-CoA: 10.8
trans-1,2-CPDA: 6.6
3-Methylbutyryl-CoA: 5.0

PKS_AT (1548..1841): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.1
Ethylmalonyl-CoA: 197.2
Methoxymalonyl-CoA: 171.6
2-Methylbutyryl-CoA: 142.7
trans-1,2-CPDA: 136.6
Propionyl-CoA: 112.6
CHC-CoA: 105.4
Malonyl-CoA: 104.5
Isobutyryl-CoA: 72.1
fatty_acid: 52.0
Benzoyl-CoA: 48.8
Acetyl-CoA: 48.3
inactive: 30.9
3-Methylbutyryl-CoA: 30.2

Link to PARAS substrate predictor