BGC0002434 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002434 (Kitasatospora sp)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	19,176	High	clifednamide D/clifednamide E/clifednamide F/clifednamide G/clifednamide H/clifednamide I/clifednamide J	PKS+NRPS:Type I

BGC0002434 - Region 1 - NRPS,T1PKS

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Location: 1 - 19,176 nt. (total: 19,176 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 19,177
Label		BGC0002434

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

cftA: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

cftC: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

cftD: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Ligases: synthase

cftB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002434.2	2.00	NRPS, PKS	clifednamide D, clifednamide E, clifednamide F, clifednamide G, clifednamide H, clifednamide I, clifednamide J	Kitasatospora sp.
BGC0001275.3	0.92	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.91	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.89	PKS	asperlactone	Aspergillus ochraceus
BGC0002192.3	0.66	PKS	FR901512	fungal sp. No.14919
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.65	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001258.3	0.65	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001553.5	0.64	NRPS, PKS	clifednamide A	Streptomyces sp. JV178
BGC0001268.4	0.63	NRPS, PKS	fusarin C	Fusarium fujikuroi

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002434.2	1.00	NRPS, PKS	clifednamide D, clifednamide E, clifednamide F, clifednamide G, clifednamide H, clifednamide I, clifednamide J	Kitasatospora sp.
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0002192.3	0.66	PKS	FR901512	fungal sp. No.14919
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001553.5	0.65	NRPS, PKS	clifednamide A	Streptomyces sp. JV178
BGC0001268.4	0.65	NRPS, PKS	fusarin C	Fusarium fujikuroi

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 19176:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - Orn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

cftB: mal - Orn

Search NORINE for peptide: strict or relaxed

PKS_AT (603..904): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; CE-Malonyl-CoA: 54.2%; Acetyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 120.2
Methylmalonyl-CoA: 119.0
Methoxymalonyl-CoA: 113.4
Ethylmalonyl-CoA: 100.5
Isobutyryl-CoA: 89.7
Acetyl-CoA: 71.5
Propionyl-CoA: 70.7
2-Methylbutyryl-CoA: 57.9
Benzoyl-CoA: 57.7
trans-1,2-CPDA: 45.7
fatty_acid: 44.2
3-Methylbutyryl-CoA: 39.1
CHC-CoA: 37.3
inactive: 33.1

AMP-binding (2352..2768): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEVGSIDK
Nearest Stachelhaus code(s):: Orn DVGEVGSIDK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor