BGC0002440 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002440 (Streptomyces sp)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,betalactone	1	50,466	longicatenamide B/longicatenamide C/longicatenamide A/longicatenamide D	NRPS:Type I	100%

BGC0002440 - Region 1 - NRPS,betalactone

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Location: 1 - 50,466 nt. (total: 50,466 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
betalactone: (HMGL-like and AMP-binding)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 50,467
Label		BGC0002440

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002440	1.51	NRP	longicatenamide B, longicatenamide C, longicatenamide A, longicatenamide D	Streptomyces sp.
BGC0002017	0.68	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0002135	0.66	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002437	0.63	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001133	0.63	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000457	0.63	NRP	vicibactin	Rhizobium etli CFN 42
BGC0002518	0.62	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001833	0.61	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825	0.58	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135	0.57	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002440	1.00	NRP	longicatenamide B, longicatenamide C, longicatenamide A, longicatenamide D	Streptomyces sp.
BGC0001133	0.74	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000426	0.70	NRP	sevadicin	Paenibacillus larvae
BGC0002017	0.69	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0002135	0.68	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002437	0.67	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001925	0.67	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0002518	0.67	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001833	0.66	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0000343	0.65	NRP	enterobactin	Pseudomonas sp. J465

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 505 - 50466:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - X) + (D-Val - D-Orn - X) + (D-Phe)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

QUJ09166.1

Search NORINE for peptide: strict or relaxed

AMP-binding (501..903): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQAGLIWK
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1560..1952): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPLVLGHVLK
Nearest Stachelhaus code(s):: Phe DPLVVGAVVK (59% 8Å match); aIle DALVFGVVLK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

QUJ09167.1

Search NORINE for peptide: strict or relaxed

AMP-binding (518..923): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWLGGTFK
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2039..2445): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DVSEAGSVEK
Nearest Stachelhaus code(s):: Lys DVSDAGGVEK (82% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (3568..3970): X

nrpys: Orn, OH-Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DANQAGFVDK
Nearest Stachelhaus code(s):: Lys DADDAGTVDK (65% 8Å match); Gln DAWQVGVVDK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

QUJ09168.1

Search NORINE for peptide: strict or relaxed

AMP-binding (520..910): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DGWSLVGIGK
Nearest Stachelhaus code(s):: Phe DAWVLAGIQK (56% 8Å match)
Stachelhaus code match:: 60% (weak)