BGC0002440 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002440 (Streptomyces sp)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,betalactone	1	50,466	High	longicatenamide B/longicatenamide C/longicatenamide A/longicatenamide D	NRPS:Type I

BGC0002440 - Region 1 - NRPS,betalactone

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Location: 1 - 50,466 nt. (total: 50,466 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
betalactone: (HMGL-like and AMP-binding)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 50,467
Label		BGC0002440

Tailoring functions

Oxidoreductases: oxygenase

QUJ09163.1: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

QUJ09170.1: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

QUJ09171.1: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acyltransferase aminotransferase

QUJ09151.1: aminotransferase

smcogs: SMCOG1019: aminotransferase

QUJ09162.1: aminotransferase

smcogs: SMCOG1019: aminotransferase

QUJ09167.1: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Hydrolases: hydrolase

QUJ09165.1: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Isomerases: isomerase

QUJ09149.1: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

QUJ09150.1: carbamoyl phosphate synthase small subunit

smcogs: SMCOG1249: carbamoyl phosphate synthase small subunit

QUJ09154.1: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

QUJ09159.1: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

QUJ09166.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

QUJ09168.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002440.3	1.45	NRPS	longicatenamide B, longicatenamide C, longicatenamide A, longicatenamide D	Streptomyces sp.
BGC0002135.2	0.85	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.3	0.74	NRPS	AM-toxin	Alternaria alternata
BGC0001166.5	0.73	NRPS	HC-toxin	Alternaria jesenskae
BGC0001825.4	0.73	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0000342.4	0.72	NRPS	enniatin	Fusarium equiseti
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.72	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002440.3	0.98	NRPS	longicatenamide B, longicatenamide C, longicatenamide A, longicatenamide D	Streptomyces sp.
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001133.4	0.71	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 505 - 50466:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - X) + (D-Val - D-Orn - X) + (D-Phe)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

QUJ09166.1: Gly - X

Search NORINE for peptide: strict or relaxed

AMP-binding (501..903): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQAGLIWK
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1560..1952): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPLVLGHVLK
Nearest Stachelhaus code(s):: Phe DPLVVGAVVK (59% 8Å match); aIle DALVFGVVLK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

QUJ09167.1: Val - Orn - X

Search NORINE for peptide: strict or relaxed

AMP-binding (518..923): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWLGGTFK
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2039..2445): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DVSEAGSVEK
Nearest Stachelhaus code(s):: Lys DVSDAGGVEK (82% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (3568..3970): X

nrpys: Orn, OH-Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DANQAGFVDK
Nearest Stachelhaus code(s):: Lys DADDAGTVDK (65% 8Å match); Gln DAWQVGVVDK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

QUJ09168.1: Phe

Search NORINE for peptide: strict or relaxed

AMP-binding (520..910): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DGWSLVGIGK
Nearest Stachelhaus code(s):: Phe DAWVLAGIQK (56% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor