BGC0002446 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002446 (Streptomyces sp)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	101,096	High	ionostatin	PKS

BGC0002446 - Region 1 - T1PKS

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Location: 1 - 101,096 nt. (total: 101,096 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 101,097
Label		BGC0002446

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

ionAIV: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

ionAVII: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

ionCI: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

ionCII: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase

ionAI: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ionAII: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ionAVI: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

ionAVIII: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: synthase

ionAIII: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ionAV: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002446.3	1.00	PKS	ionostatin	Streptomyces sp.
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000067.5	0.67	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002446.3	1.00	PKS	ionostatin	Streptomyces sp.
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000067.5	0.67	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 101096:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ccmal - redmal - Me-ccmal - redmal - ohmal - Me-ohmal) + (Me-ohmal) + (ccmal) + (Me-redmal - Me-redmal) + (ohmal - Me-mal) + (Me-redmal - Me-redmal) + (Me-redmal)
Non-colinear ordering:: ionAI + ionAII + ionAIII + ionAIV + ionAV + ionAVI + ionAVII

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ionAI: mal - mmal - mal - mmal - mal - mal - mmal

PKS_AT (550..851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 229.3
inactive: 102.9
Methoxymalonyl-CoA: 63.6
Methylmalonyl-CoA: 51.9
Propionyl-CoA: 30.8
Isobutyryl-CoA: 30.3
Ethylmalonyl-CoA: 28.5
Benzoyl-CoA: 27.0
Acetyl-CoA: 21.4
2-Methylbutyryl-CoA: 17.9
fatty_acid: 11.7
CHC-CoA: 4.9
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1597..1893): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 183.7
Ethylmalonyl-CoA: 156.2
Methoxymalonyl-CoA: 146.1
2-Methylbutyryl-CoA: 100.0
Malonyl-CoA: 99.8
Propionyl-CoA: 90.6
trans-1,2-CPDA: 87.0
Isobutyryl-CoA: 61.4
CHC-CoA: 56.1
Benzoyl-CoA: 48.7
3-Methylbutyryl-CoA: 39.3
Acetyl-CoA: 26.7
fatty_acid: 26.4
inactive: 18.4

PKS_AT (3355..3654): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.0
inactive: 102.4
Methoxymalonyl-CoA: 56.9
Methylmalonyl-CoA: 52.7
Isobutyryl-CoA: 33.6
Propionyl-CoA: 29.8
Benzoyl-CoA: 21.8
Ethylmalonyl-CoA: 18.0
Acetyl-CoA: 14.8
fatty_acid: 14.5
trans-1,2-CPDA: 10.6
2-Methylbutyryl-CoA: 8.5
CHC-CoA: 6.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (5451..5747): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 192.1
Ethylmalonyl-CoA: 164.1
Methoxymalonyl-CoA: 147.2
2-Methylbutyryl-CoA: 116.0
Propionyl-CoA: 114.8
Malonyl-CoA: 102.3
trans-1,2-CPDA: 98.6
CHC-CoA: 91.4
Benzoyl-CoA: 44.1
Isobutyryl-CoA: 43.8
fatty_acid: 40.6
Acetyl-CoA: 38.2
inactive: 34.4
3-Methylbutyryl-CoA: 22.5

PKS_AT (7220..7519): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.0
inactive: 102.4
Methoxymalonyl-CoA: 56.9
Methylmalonyl-CoA: 52.7
Isobutyryl-CoA: 33.6
Propionyl-CoA: 29.8
Benzoyl-CoA: 21.8
Ethylmalonyl-CoA: 18.0
Acetyl-CoA: 14.8
fatty_acid: 14.5
trans-1,2-CPDA: 10.6
2-Methylbutyryl-CoA: 8.5
CHC-CoA: 6.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (9303..9593): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 230.1
inactive: 122.9
Methoxymalonyl-CoA: 64.4
Methylmalonyl-CoA: 52.0
Isobutyryl-CoA: 27.9
Propionyl-CoA: 26.9
Acetyl-CoA: 23.9
Benzoyl-CoA: 19.3
Ethylmalonyl-CoA: 18.8
2-Methylbutyryl-CoA: 10.7
CHC-CoA: 8.4
trans-1,2-CPDA: 8.3
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (10857..11153): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 190.5
Ethylmalonyl-CoA: 162.2
Methoxymalonyl-CoA: 148.7
2-Methylbutyryl-CoA: 110.0
Propionyl-CoA: 108.9
trans-1,2-CPDA: 101.8
Malonyl-CoA: 96.5
CHC-CoA: 88.9
Benzoyl-CoA: 45.7
Isobutyryl-CoA: 44.1
fatty_acid: 35.7
Acetyl-CoA: 34.8
inactive: 34.4
3-Methylbutyryl-CoA: 24.7

ionAII: mmal

PKS_AT (578..874): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 192.5
Ethylmalonyl-CoA: 165.8
Methoxymalonyl-CoA: 149.9
Propionyl-CoA: 115.2
2-Methylbutyryl-CoA: 113.9
Malonyl-CoA: 101.3
trans-1,2-CPDA: 98.3
CHC-CoA: 87.8
Isobutyryl-CoA: 48.9
Benzoyl-CoA: 45.9
fatty_acid: 40.4
Acetyl-CoA: 40.3
inactive: 33.7
3-Methylbutyryl-CoA: 23.4

ionAIII: mal

PKS_AT (592..891): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.0
inactive: 102.4
Methoxymalonyl-CoA: 56.9
Methylmalonyl-CoA: 52.7
Isobutyryl-CoA: 33.6
Propionyl-CoA: 29.8
Benzoyl-CoA: 21.8
Ethylmalonyl-CoA: 18.0
Acetyl-CoA: 14.8
fatty_acid: 14.5
trans-1,2-CPDA: 10.6
2-Methylbutyryl-CoA: 8.5
CHC-CoA: 6.7
3-Methylbutyryl-CoA: 0.0

ionAIV: mmal - mmal

PKS_AT (572..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.2
Ethylmalonyl-CoA: 164.6
Methoxymalonyl-CoA: 146.7
Propionyl-CoA: 113.2
2-Methylbutyryl-CoA: 112.6
Malonyl-CoA: 100.4
trans-1,2-CPDA: 99.0
CHC-CoA: 88.8
Isobutyryl-CoA: 45.6
Benzoyl-CoA: 44.1
fatty_acid: 38.3
Acetyl-CoA: 38.0
inactive: 34.3
3-Methylbutyryl-CoA: 21.6

PKS_AT (2672..2968): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.0
Ethylmalonyl-CoA: 162.9
Methoxymalonyl-CoA: 146.4
Propionyl-CoA: 111.9
2-Methylbutyryl-CoA: 111.2
Malonyl-CoA: 100.5
trans-1,2-CPDA: 98.3
CHC-CoA: 87.8
Isobutyryl-CoA: 43.4
Benzoyl-CoA: 42.8
Acetyl-CoA: 37.7
fatty_acid: 37.4
inactive: 34.0
3-Methylbutyryl-CoA: 21.6

ionAV: mal - mmal

PKS_AT (581..880): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.0
inactive: 102.4
Methoxymalonyl-CoA: 56.9
Methylmalonyl-CoA: 52.7
Isobutyryl-CoA: 33.6
Propionyl-CoA: 29.8
Benzoyl-CoA: 21.8
Ethylmalonyl-CoA: 18.0
Acetyl-CoA: 14.8
fatty_acid: 14.5
trans-1,2-CPDA: 10.6
2-Methylbutyryl-CoA: 8.5
CHC-CoA: 6.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (2143..2443): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 87.5%; Methylmalonyl-CoA: 87.5%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 178.8
Ethylmalonyl-CoA: 161.0
Methoxymalonyl-CoA: 154.2
2-Methylbutyryl-CoA: 109.6
CHC-CoA: 94.9
Propionyl-CoA: 94.4
trans-1,2-CPDA: 92.5
Malonyl-CoA: 91.1
Isobutyryl-CoA: 73.6
Benzoyl-CoA: 63.2
Acetyl-CoA: 45.0
3-Methylbutyryl-CoA: 36.0
fatty_acid: 26.9
inactive: 21.6

ionAVI: mmal - mmal

PKS_AT (581..877): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.2
Ethylmalonyl-CoA: 164.6
Methoxymalonyl-CoA: 146.7
Propionyl-CoA: 113.2
2-Methylbutyryl-CoA: 112.6
Malonyl-CoA: 100.4
trans-1,2-CPDA: 99.0
CHC-CoA: 88.8
Isobutyryl-CoA: 45.6
Benzoyl-CoA: 44.1
fatty_acid: 38.3
Acetyl-CoA: 38.0
inactive: 34.3
3-Methylbutyryl-CoA: 21.6

PKS_AT (2679..2975): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.0
Ethylmalonyl-CoA: 162.9
Methoxymalonyl-CoA: 146.4
Propionyl-CoA: 111.9
2-Methylbutyryl-CoA: 111.2
Malonyl-CoA: 100.5
trans-1,2-CPDA: 98.3
CHC-CoA: 87.8
Isobutyryl-CoA: 43.4
Benzoyl-CoA: 42.8
Acetyl-CoA: 37.7
fatty_acid: 37.4
inactive: 34.0
3-Methylbutyryl-CoA: 21.6

ionAVII: mmal

PKS_AT (569..865): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 192.3
Ethylmalonyl-CoA: 164.1
Methoxymalonyl-CoA: 149.6
Propionyl-CoA: 113.9
2-Methylbutyryl-CoA: 112.5
Malonyl-CoA: 101.4
trans-1,2-CPDA: 97.5
CHC-CoA: 86.7
Isobutyryl-CoA: 46.8
Benzoyl-CoA: 44.6
Acetyl-CoA: 40.0
fatty_acid: 39.6
inactive: 33.4
3-Methylbutyryl-CoA: 23.4

Link to PARAS substrate predictor