LOCUS       BGC0002449             14377 bp    DNA     linear   ENV 16-MAR-2020
DEFINITION  Uncultured bacterium sterol biosynthesis gene cluster, complete
            sequence.
ACCESSION   BGC0002449
VERSION     BGC0002449
KEYWORDS    .
SOURCE      uncultured bacterium
  ORGANISM  uncultured bacterium
            Bacteria; environmental samples.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:44:02
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..14377
                     /collection_date="29-Jun-2015"
                     /db_xref="taxon:77133"
                     /environmental_sample=""
                     /host="Melophlus sp."
                     /isolation_source="GUM22 sponge holobiont tissue"
                     /lat_lon="13.4513 N 144.656 E"
                     /mol_type="genomic DNA"
                     /organism="uncultured bacterium"
     protocluster    1..14377
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[7173:10635]"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      7174..10635
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     cand_cluster    1..14377
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14377
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002449"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14377
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(Terpene_synth or Terpene_synth_C or phytoene_synt
                     or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps
                     or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(48..575)
                     /locus_tag="msb_00001"
     CDS             complement(48..575)
                     /codon_start=1
                     /locus_tag="msb_00001"
                     /product="nicotinamide-nucleotide amidohydrolase PncC"
                     /protein_id="QIJ55903.1"
                     /transl_table=11
                     /translation="MREHLSNGYDCDMSDSALLEAAKVLADHSLAKGLRVTVAESCTGG
                     WLAKCCTDLPGSSDWFECGWVTYSNEAKSQSLGVDPELIRQEGAVSQAVVEAMAAGALE
                     RAQCDLACAISGVAGPSGGTESAPVGCVWFAFLRTGEEMRSERRDFPGGRDTVRRESVL
                     HALRRLAEEISL"
     misc_feature    48..14377
                     /note="sterol biosynthesis gene cluster msb"
     gene            627..1160
                     /locus_tag="msb_00002"
     CDS             627..1160
                     /codon_start=1
                     /locus_tag="msb_00002"
                     /product="hypothetical protein"
                     /protein_id="QIJ55904.1"
                     /transl_table=11
                     /translation="MNTPIISFRVRQDQQTDVRELVNSVKDDPELLGLVLEYLQNDPRP
                     THEAAVADGNLGPFRNAEAALSVLTMNLAIAFNPDAIFLFGSRACGTARSDSDFDLMVV
                     TPDDQPLDYLTARKPISGCGVPVDVVPCRYSAFEKNRKVPGMLPYAVDREGKLLDARID
                     GPFWKRYRETYPLR"
     gene            1157..1546
                     /locus_tag="msb_00003"
     CDS             1157..1546
                     /codon_start=1
                     /locus_tag="msb_00003"
                     /product="hypothetical protein"
                     /protein_id="QIJ55905.1"
                     /transl_table=11
                     /translation="MTSPLVLGFLKTAERDIAGIERLLPDLPDLAAFHAQQATEKLARA
                     VCLHEGLPDLRTHDISRITEQLAQEHPLRKNLVKLDSLTGAATAWRYPDAEGGFPRPPG
                     PSEIQAALEQIKNIQAEIHDWLSVL"
     gene            1859..2188
                     /locus_tag="msb_00004"
     CDS             1859..2188
                     /codon_start=1
                     /locus_tag="msb_00004"
                     /product="hypothetical protein"
                     /protein_id="QIJ55906.1"
                     /transl_table=11
                     /translation="MSQTAKRFEAASDAQKKVTPPNFDTLQAVKNLKKAEFGDKQAVTL
                     VETLQDAQSELATKADLAEMEFRLRGEIEARFNKLSWHILAVAGGATGTITGILTLVIL
                     FAPAK"
     gene            2413..3588
                     /gene="msbA"
                     /locus_tag="msb_00005"
     CDS             2413..3588
                     /NRPS_PKS="Domain: NAD_binding_4 (81-201). E-value:
                     5.9e-11. Score: 33.8. Matches aSDomain:
                     nrpspksdomains_msb_00005_NAD_binding_4.1"
                     /codon_start=1
                     /gene="msbA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score: 179;
                     E-value: 2.5e-54)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="msb_00005"
                     /product="3 beta-hydroxysteroid dehydrogenase/delta
                     5-->4-isomerase"
                     /protein_id="QIJ55907.1"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 7.6e-14,
                     bitscore: 41.0, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 6.1e-09, bitscore:
                     24.9, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGKVSESGHKMTSGSSTPNNSSAAPVFVTGAAGHVGANLVRRLL
                     DEGTWVRVLLRHEENNEALEGLEVERVFGDLRDLNSVRRAVEGCQGVYHCAAKVSTIEG
                     NHAHRREIYECNVLGTRNMLQAARETEAGRVVVTGSFSAVGYNLDDPSAPSDETMLFYP
                     MERTMPYERSKTLVEQVCWRAAAAGQDVIVATCCAVVGGSDFFPSRLGRTLCDYTNGKL
                     HAYVDGGFEFVAARDIVEGHRLCMDRGRTGEKYIFSSEYKTISDIIDLYEEVSGIPRPH
                     RRIPVRAMLAFSEVASFYLSRFHPSFPQRFTPGAIRLLQKCRHADTSKAKEELGYRPTS
                     IRDAVHEAYAFHYHQRKAITNPDAKPPCTDGMRQIEELMPDQGTPITSDGG"
     aSDomain        2656..3015
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_msb_00005_NAD_binding_4.1"
                     /evalue="5.90E-11"
                     /label="msb_00005_NAD_binding_4.1"
                     /locus_tag="msb_00005"
                     /protein_end="201"
                     /protein_start="81"
                     /score="33.8"
                     /tool="antismash"
                     /translation="VRRAVEGCQGVYHCAAKVSTIEGNHAHRREIYECNVLGTRNMLQA
                     ARETEAGRVVVTGSFSAVGYNLDDPSAPSDETMLFYPMERTMPYERSKTLVEQVCWRAA
                     AAGQDVIVATCCAVVG"
     gene            3590..4687
                     /gene="msbB"
                     /locus_tag="msb_00006"
     CDS             3590..4687
                     /codon_start=1
                     /gene="msbB"
                     /locus_tag="msb_00006"
                     /product="toluene-4-sulfonate monooxygenase system
                     iron-sulfur subunit"
                     /protein_id="QIJ55908.1"
                     /transl_table=11
                     /translation="MDSMTSPPPGFDEASNLRQKARAAGMDPDYWYAVEEVRRVKRGTV
                     VEVVFWKHAIALFRAKDGSFHAVEDRCAHRQLKLSLGHVEDCRLVCAYHGWQYDGGGQR
                     AGGPREHLGESCENVSIRSYPVKVRYGLVWLFPGDPEKAESRQIPDIPELEGPDRWACV
                     PLAFTWSAHHSMVIDNVSDFSHAHLHRDYRPFVGATLTRCETIGDDVHLSYDTQVGRGR
                     ISGLFVDHKKLDTNHMDLCYQYPYQWSNTDDWIKHWLFVLPIDERTTRTFFLFYFKSLK
                     IPLLPVHIPRFAMTFVLKISNRILIGPLLTQDQYALEGEQEGYEKHWDAPTVEFNPVVE
                     AFQDVTVRKWREYLTRKAENGSAYA"
     gene            4680..5774
                     /gene="msbC"
                     /locus_tag="msb_00007"
     CDS             4680..5774
                     /codon_start=1
                     /gene="msbC"
                     /locus_tag="msb_00007"
                     /product="hypothetical protein"
                     /protein_id="QIJ55909.1"
                     /transl_table=11
                     /translation="MPELPFLLTALSGSTLLDAAAMMLAFFGFLFLGSMLLPGRRITGP
                     DYDGKSRVYRSNGLALFLLTMIVGGLAQVSGWFSLSVLYTHFLALFVVANVFAFAFSAL
                     LYYQGTRAQGDSPRGLEGFFYGVEGHPTWFGVDLKFFSYRPSLIGLALFNVSFAAVQYE
                     TYGELTLAMMLYQAFTFLYVLNYFHFEYGMVHTWDIVSERFGWMLVWGDYVLVPFFYCL
                     AGWWLVHATDPLPPVAAVAITLLFVFGFWIFRGANGQKHRFKRDSHATIWGKPAESLDG
                     RLLVSGFWGIGRHLNYTGEICIYFSFALTTGFASWVPYLLPAWLVGLLSHRSRRDDRRC
                     RAKYGELWERYTKRVRFAMLPFVY"
     gene            5789..7177
                     /gene="msbD"
                     /locus_tag="msb_00008"
     CDS             5789..7177
                     /codon_start=1
                     /gene="msbD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 291.1; E-value: 2.7e-88)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="msb_00008"
                     /product="lanosterol 14-alpha demethylase"
                     /protein_id="QIJ55910.1"
                     /sec_met_domain="p450 (E-value: 8.8e-67, bitscore: 215.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVRSTPLSPQEARAIPEVAGKWPLIGHLLDFQKDPIAMLQRGW
                     REQGELIRFRLGPREFFLFTGPEAHDFYFHAPEDQLSAKEVYQFTVPIFGRGVAYDTTP
                     ELMLEQLGFLYPALNESAMRRFARIMFKETWQFAEDLGESGEVDLPHAMNELTVKIASR
                     CLIGEEVRAQLDAGFAEAYHELQNGINTLGFFFPYLPIPAHRRRDRARRKVAKLFGQIM
                     AERRRTEAQPEDFMQTLMNARYKDGHAPSDDEITGILLTVLFAGQHTSAVLATWTLLEL
                     MRTPSYLARVKDEVQDTYREAETVSYESLRHQVTLEHAIRECERMHPPLIMLIRKVLRP
                     LHYKDHVVPAGALAMVSPAVSQRLPHLFADPNRFNPDRFAPPASEGKQHHYALISFGGG
                     KHRCIGEHFAYMQLKAIWTVLFDRFDFEPITEFPEPNYGSWVTGPREPCRVRYRRRPQA
                     SIFQ"
     gene            7174..10635
                     /gene="msbE"
                     /locus_tag="msb_00009"
     CDS             7174..10635
                     /codon_start=1
                     /gene="msbE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: terpene_cyclase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1161:oxidoreductase (Score: 93.6; E-value: 2.1e-28)"
                     /gene_kind="biosynthetic"
                     /locus_tag="msb_00009"
                     /product="squalene--hopene cyclase"
                     /protein_id="QIJ55911.1"
                     /sec_met_domain="terpene_cyclase (E-value: 8.7e-17,
                     bitscore: 50.9, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIPPSRHYDVAIVGGGPVGSVCALAHARKGARVVLLEASPEASK
                     RLAGEWLHPPAVRILREIGIGLDTQPPIGAGNGFVVYPEDDSEPIILPYPDGAHGLACE
                     HAVLVSHLHEAIENESSIDFIFNAQVREVVDGRITFTRNGADESVAAARIVGADGRGSV
                     VRRSLGLPTNQKTCSWMVGVLVDDVTLPLEGYGYVLGGGPGPILMYRLGERGVRIVVDV
                     PADRWTPRDRIGFLLDSYTSWLPESVRPAFVDALQAGQFRTASNKLRLRVTYGNPHRVL
                     IGDAAGHYHPMSAVGITLGFGDALALAESGDFRDFTKKRFQAIRAPEFLAMGLYEVFAD
                     HRAEVVALRHATYRGWRASSTMRDRTMRLLACENTSVIHLSSAFLTTMTRAVAGEIPRS
                     FDKLAWRRARDTFNSLSGRLKWLLRGARMLHRVRSTGEGEDEQIRDTWSHTFLVSIPSK
                     TDDSQPARPWTIESPDAGPALKSASKRLLDLQDGVGAWEGEMVWCPMLTAQYVLLHYIM
                     GQPLDPDRRRRVLRSFEQTRLEGGAWGLHEHPPPNLFVTTLVYVAARLLGVERDDSLLK
                     PAQEFLQTEGVLGIPSWGKFWLALLNLYDWRGINAILPELWSLPRWIPLHPSNWYCHTR
                     LIYMAMAAVYSHRFQAPVTPLIASLREELYPEGFANVGFSAGRNRLRNADLHARPSVWL
                     RIGYGFSQLFERFHSKYLRARCMDAITERIQWELRTTNHTSISPVSGLLNILALWLRDP
                     NDPDSRQALAKLDDWIWEDEEYGARVTGARSISWDTGFALQALEAMSSLDLAPEVRNSL
                     QRGADFLETQQIKASFEGFREAFRNDPKGGWCFAGQWHGWPVTDCTAEAVLGIIAAHRE
                     ATNTAMLKDAIQFMLRGQNRDGGFGSYEAQRSVIGLEWLNPAEMFGESMTEHSYVECTA
                     SCLAALAACKKHFPDTTDQAVTDAMSRASAWLRRTQASDGSWRGVWGVQFIYGTSFGIR
                     GLLAAGARPGDPALRLACRWLLDRQRGDGGWGEHYSGCFTGQYIAHDESQVVQTAWALI
                     ALLEADDPNWAAISRGARFLLDTQNADGAWPKQDMAGVFFRTALLDYVLYRQYFPLHAL
                     ALYEQRRRTRLELAAIDSLTAETTANSETPLAAATLEEPHSAHVPS"
     gene            10663..11697
                     /gene="msbF"
                     /locus_tag="msb_00010"
     CDS             10663..11697
                     /NRPS_PKS="Domain: oMT (88-196). E-value: 4.3e-23. Score:
                     72.9. Matches aSDomain: nrpspksdomains_msb_00010_oMT.1"
                     /codon_start=1
                     /gene="msbF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 146.6; E-value:
                     1.5e-44)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="msb_00010"
                     /product="sterol 24-C-methyltransferase"
                     /protein_id="QIJ55912.1"
                     /sec_met_domain="PCMT (E-value: 1.1e-08, bitscore: 24.5,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLTPAKRSDSRSLADLTPDEVIQVVNHYAGLHDADLEDRKEDYQ
                     SLVNHYYDLVTDFYEFGWGQSFHFAPRHRGESFKESLLRHEHFLADQLSLKPGMQVLDV
                     GCGIGGPMRVLAHYSGASFVGINNNAYQIERAKVHTEDVRSLCRFIRGDYMQIPEDDNC
                     YDAAFAIESMPHAPNKTAAFREILRILRPGSCFTGYEWCLTDAFDPENTEHLRIKKVIE
                     RGNGLPDIAATSEVCDALRMAGFEILDARDLAPESHPETPWYRALQGRDLSLASVPRTP
                     LGRALTNLILRVGEKLRVFPEGTRMVSTLLNRGADALVEGGETGIFTPMFFFLARKPKH
                     SGTE"
     aSDomain        10927..11250
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_msb_00010_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="4.30E-23"
                     /label="msb_00010_oMT.1"
                     /locus_tag="msb_00010"
                     /protein_end="196"
                     /protein_start="88"
                     /score="72.9"
                     /tool="antismash"
                     /translation="LADQLSLKPGMQVLDVGCGIGGPMRVLAHYSGASFVGINNNAYQI
                     ERAKVHTEDVRSLCRFIRGDYMQIPEDDNCYDAAFAIESMPHAPNKTAAFREILRILRP
                     GSCF"
     gene            11862..>14377
                     /locus_tag="msb_00011"
     CDS             11862..>14377
                     /codon_start=1
                     /locus_tag="msb_00011"
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="QIJ55913.1"
                     /transl_table=11
                     /translation="MRQYLEFKAQYPQHLLLYRMGDFYELFFEDAKRGSELLDIVLTER
                     GRSKGQPIPMAGIPVVSLDSYLAKLVDLGESAVVCEQVGDSPSGRGPMERQISRIVTPG
                     TLIEDELLDAGRDTLLLALWPGDPIGLASLNLSSGRFNVMELPSIEDLYAELERLRPAE
                     ILYPESNTPPDWEDTHWQPLSDWHFDVETATRLLCQQLGVHDLSAFDCQDAPSATGAAG
                     ALLYYAQETQRAALPHITSLQRERRDTHIYLDATSRRNLELEQTISGDSRHSLLGVLDK
                     TVTAMGRRCLSRWIQSPLRDRETLATRHQIVSHLLDNTHRAPIREQLRKVSDIERVIAR
                     LALYTASPRDLCGLAQTLQRLPNLRRLLENIDAAEFDSYRTHLQSDPELATHLASALAE
                     NPPPTIREGGAIAAGFDSELDELRELSRGADDFLLEMEATERESTGIAQLKVAYNRVHG
                     YYIEVPRSQAARVPDHYRRRQTLKNYERYLTSELKEFEDRVLSARSRALEREKQVYQNL
                     LESLTPHLNHFKQCAAALSELDVLCSFAEQAKAHHYCCPELVDEPGVEIEAGRHPVIEL
                     TWNEPFVPNDTLLSEQRRLLLVTGPNMGGKSTYMRQTALIVLMACIGSHVPAKQARIGP
                     IDRIFTRIGASDDLTSGRSTFMVEMTEAANILHHASEYSLVLMDEIGRGTSTFDGMALA
                     LGCAEYLATQNRAFTLFSTHYFELTQLAEIHPGIVNLHLDATMHGQQIVFLHAVKEGPA
                     SQSYGLQVARLAGIPAPVLERAQAYLLELEDDSTQRWARSNPQKDLFVPPSDPPSVPAA
                     LERLRQVKPDQLSPKEALDLIYELQKL"
ORIGIN
        1 tccacacggg ccgccccgga ttaatccggg cttgatcggc cctgaaacta cagggaaatc
       61 tcttccgcca atcgacgcag cgcgtgcagc acagactccc gtcggactgt atcgcgaccc
      121 ccagggaagt ctcgccgttc gctgcgcatc tcctcgcccg tccgcaggaa tgcaaaccac
      181 acgcaaccga ctggcgcact ctccgtcccg ccggatggtc cggccacgcc gctgatcgca
      241 caggccaagt cgcactgcgc acgctccagg gctcctgctg ccatcgcctc gaccacggcc
      301 tgggaaactg cgccctcttg gcggattagt tccggatcga caccaagcga ttggcttttc
      361 gcttcgttgc tgtaggttac ccacccgcat tcgaaccagt cggagctgcc cggcaggtcg
      421 gtgcagcatt tggccagcca gcccccggta caggattcgg ctaccgtcac gcgcagaccc
      481 ttcgctaggc tgtggtcggc caacactttt gccgcttcca acagtgctga gtcactcatg
      541 tcgcaatcat acccgtttga cagatgttcc cgcatgcttt acatttctca aagtgtatta
      601 cagggtgtaa tacacaggag gattccatga atacccccat catcagtttc cgcgtacgcc
      661 aggatcagca aaccgatgtg cgcgaattgg tcaattcagt aaaggatgac ccggaactac
      721 tcggcctagt gctggaatac cttcaaaatg accctcggcc aacacatgag gccgcagtgg
      781 cagatgggaa tcttggtcca ttccgcaacg cggaagctgc cctttccgtt ctgaccatga
      841 atctggctat cgcattcaat ccggatgcca ttttcctgtt tggcagccgc gcatgcggca
      901 ctgcgcgctc ggacagtgac ttcgacctga tggtggtcac cccggatgac caacctctcg
      961 attacctgac ggcccgaaaa cccatttccg ggtgtggtgt gccggtggac gtagttcctt
     1021 gccggtattc cgcgttcgag aagaaccgga aagtacccgg catgctccct tacgcggtcg
     1081 accgggaggg gaaattgctg gacgcgcgca ttgatggtcc attttggaaa cgttatcgcg
     1141 agacgtaccc tctgcgatga cctctccgct cgtcctggga ttcctgaaga ctgccgaacg
     1201 ggatatagcc ggcattgagc gccttcttcc agatcttcct gacctggcgg ctttccatgc
     1261 tcaacaggct acagaaaaac tcgcacgtgc cgtgtgcctg cacgaagggc tcccggatct
     1321 tcgcacccat gacatctcgc gcattactga acaactagcc caagaacatc ctctcaggaa
     1381 aaacctcgtc aaactcgaca gcctcactgg agcggcaacc gcttggcgtt atccagatgc
     1441 tgaaggagga tttccgagac ctcctgggcc ttctgaaatc caagctgcgc ttgaacagat
     1501 aaaaaacatc caagccgaaa tccatgactg gctctcggtt ctgtgaatcc ccctctggaa
     1561 gctcgtagta cagtcatctg cagtctgacc agttcggcca agtcgaaaat ttcacactct
     1621 ccagaaatcg acgagttcat gaaaccaaaa acacgaaaaa cttccaaaaa tccggctcgc
     1681 aagtattcgg tttcatctct aaccgtttga ttctaattta gaaaaaacgg tctaaaaatt
     1741 ccgaccattt ttccttgatt ttcgccgatc tcatggcatc atcccgaacc gagccatgga
     1801 gaccctcgat ggaaagagcc tccccacatt catcccgccc ccgtcaagga gaaccgatat
     1861 gagccagact gcaaaaaggt tcgaagccgc atccgatgcg cagaagaagg tcaccccgcc
     1921 gaactttgat acactgcagg ctgtcaagaa tctgaagaaa gcagaattcg gagacaagca
     1981 ggccgtgacc ttggtcgaaa cccttcaaga tgcgcaaagc gagttggcga cgaaggctga
     2041 cttggcggag atggaattca ggctgcgggg ggagatagaa gccagattca ataaactctc
     2101 atggcacatc ctggccgtcg cgggcggagc aaccggaacc ataaccggca ttctcacttt
     2161 agtgatcctc ttcgctcctg cgaaatagac accctcactc tcaaaaaatc ctggtaggtc
     2221 tgcgcacttc ggtgccagac cataagtttg ttagaattat gccgtcagtc atattgcgag
     2281 ggatggctgg atgcaaatcg gtgattgcga tcgcctgatt ggcttgaaac aaggcggcta
     2341 tggagcggcg aacgccggtt tatcctggcc gttgttgagc gttttgcctg cgtaccctcg
     2401 ataccgttaa acatggatgg aaaagtttcg gaatcagggc acaaaatgac ttcaggatcg
     2461 agcacaccca acaattcatc ggctgcaccc gttttcgtca ccggcgcggc cgggcatgtc
     2521 ggcgccaatc tcgtacgccg gctactcgac gaaggcacct gggtccgggt gctgctaaga
     2581 cacgaagaga acaacgaagc actggaaggc cttgaggtcg aacgcgtgtt cggtgacctg
     2641 cgggacttga attccgtcag gcgtgcggtc gagggttgcc aaggcgtcta tcactgcgcc
     2701 gccaaggttt cgaccatcga gggaaaccac gctcatcgcc gagaaatata cgagtgcaac
     2761 gtcctcggca cccgcaacat gctgcaggcg gcacgggaga cggaagccgg ccgggtggtc
     2821 gtgacgggct ctttcagcgc ggtcggatac aacctggatg acccctccgc ccccagcgac
     2881 gagacaatgc tgttctaccc tatggaacgc acgatgccct acgagcgcag caagacgctt
     2941 gttgaacagg tgtgctggcg cgccgcggcg gccggacagg atgtcatcgt cgcaacgtgt
     3001 tgcgcggttg tgggaggcag cgatttcttc ccttcacgat tgggtcggac cctgtgtgat
     3061 tacaccaacg gcaagctcca tgcctacgtc gatggcggct tcgagtttgt cgcagcccga
     3121 gacatcgttg aaggacaccg gttgtgcatg gatcgcgggc gcacgggcga gaaatacatc
     3181 ttcagttccg aatacaaaac catctccgac atcatcgacc tatacgagga agtctccggc
     3241 attccgcgac ctcaccgacg gattccggtc cgcgcgatgc tcgctttctc ggaagttgca
     3301 agtttttacc tgagccggtt tcatccaagt ttcccccagc gtttcactcc cggcgccatt
     3361 cgcctgctgc aaaagtgccg ccatgccgac accagcaagg cgaaagagga actcggctac
     3421 cggccgacca gcatccgcga cgcggttcac gaagcctacg cctttcacta ccaccagcgc
     3481 aaggcgatca cgaacccgga cgcgaaaccc ccgtgcacgg acggcatgag acaaatagaa
     3541 gaactcatgc cggatcaagg cacacccatc acttcggacg gaggctgaaa tggactcaat
     3601 gacaagcccg cctcctggct tcgacgaagc cagtaattta cgccagaaag cacgcgccgc
     3661 gggcatggac ccggattatt ggtacgcggt cgaagaagtg cgccgcgtca agcggggaac
     3721 cgtggtcgag gtcgttttct ggaaacacgc cattgcactg ttccgggcga aagacggctc
     3781 gttccatgcg gtcgaggacc gttgtgccca tcgccagctc aagctttccc tcggccatgt
     3841 cgaggactgt cggctcgtct gcgcctacca tggctggcaa tacgacggag ggggacaacg
     3901 tgccgggggg cctcgcgagc acctcgggga aagctgcgag aacgtctcga tccggtccta
     3961 ccccgtgaaa gtccgttacg gcctggtctg gttgttcccc ggggatccgg aaaaggcgga
     4021 gtcgcggcag attccggaca tccccgagct cgaggggccg gaccgatggg cctgcgtccc
     4081 gcttgctttc acctggtcgg cgcaccactc gatggtgatc gacaacgtga gcgacttctc
     4141 tcatgcccat ctgcaccgtg attaccgtcc gttcgtcggc gccaccctca cccgctgcga
     4201 gaccatcggc gacgacgtgc atctttccta cgacacgcag gtcggacgcg ggcgcatctc
     4261 cgggctgttc gtcgaccaca aaaagctcga caccaatcac atggatctct gctaccaata
     4321 cccttaccag tggtcgaaca ccgatgactg gatcaaacac tggcttttcg tgctgcccat
     4381 cgacgagcgg accacccgga ctttcttttt gttctatttc aagtcgctga agatacccct
     4441 tctgccggtg cacatccccc gtttcgccat gacctttgtt ctaaagatat cgaaccgcat
     4501 tctcatcggc ccgttgctca ctcaggatca gtacgcactc gaaggagaac aggaaggtta
     4561 cgagaaacat tgggacgctc cgaccgtcga gttcaatccc gtggtcgagg ctttccagga
     4621 cgtgaccgta cgcaagtggc gggaatacct gacacgcaaa gcggagaacg gcagcgccta
     4681 tgcctgaact gcccttcttg ctgactgcgc tgtccggcag caccctgctc gacgcggcag
     4741 ccatgatgct ggcattcttc ggcttcctgt tcctgggttc gatgctgttg cccggacgcc
     4801 ggattacggg accggattac gatggaaaat caagggtcta caggagcaac ggcctcgccc
     4861 tgttcctgct gaccatgatc gtgggagggc ttgcccaagt ctccggctgg ttttcccttt
     4921 cggtgctgta tacccacttt ctcgcgctgt tcgtggtggc gaacgtattc gcgtttgctt
     4981 tttccgcctt gctgtactac caaggcaccc gggctcaggg cgattccccg aggggattgg
     5041 aaggtttctt ctatggcgtg gaaggccatc cgacctggtt cggggtggac ttgaagtttt
     5101 tcagttaccg cccttccctc atcggcctcg cgttgttcaa cgtctcgttc gccgccgtgc
     5161 agtacgaaac ctacggcgaa ctgaccttgg ccatgatgct ttaccaggcc tttaccttcc
     5221 tgtacgtcct caactacttc catttcgaat acggaatggt tcacacctgg gacatcgtca
     5281 gcgaacgctt cggatggatg ctggtttggg gagactacgt gctggtgccg tttttctatt
     5341 gcctcgccgg ctggtggctg gttcacgcaa ccgaccccct gccccccgtc gctgccgtcg
     5401 cgatcaccct gctgttcgtc ttcggcttct ggatcttccg cggcgccaac gggcagaagc
     5461 accgtttcaa acgtgactct cacgccacga tctgggggaa acccgccgag tccctggacg
     5521 ggcgcctgct ggtctccgga ttttggggaa tcgggcggca tctcaactat accggcgaga
     5581 tctgcattta cttttcgttc gccctgacca ccggattcgc atcctgggtg ccctacctgt
     5641 tgcccgcgtg gttggtggga ctgttgtcac atcgctcaag gcgcgacgac cgccgctgcc
     5701 gcgccaagta cggcgaactc tgggagcggt acacgaagcg agtgcgtttc gcgatgctgc
     5761 cgttcgtcta ttgaggcagc ctgacgacat gaccaccgta cgttccaccc cactttcgcc
     5821 gcaggaggcc cgggccattc ccgaggtcgc gggcaagtgg ccgttgatcg gccatctgct
     5881 ggatttccag aaggacccga tcgcgatgct gcagcgcggc tggcgcgagc aaggcgaact
     5941 catccggttc cggctcggcc cgcgcgagtt tttcctcttc accgggcccg aagcccacga
     6001 tttctacttc cacgcacccg aagaccagct cagcgccaaa gaggtctacc agttcacggt
     6061 gcccatcttc gggcgcggcg tagcttacga caccacacct gaactgatgc tcgagcaatt
     6121 gggatttttg tatccggcgc tcaacgaatc cgcgatgcgc cggttcgccc gcatcatgtt
     6181 caaggagacc tggcagtttg ccgaggacct cggcgagagt ggggaagtcg atctgcccca
     6241 tgcgatgaac gaactcaccg tcaagatcgc cagccgttgc cttattggag aggaagtccg
     6301 ggcccagctg gatgcgggat tcgccgaggc ttaccacgaa ctgcagaacg gcatcaacac
     6361 cttggggttc tttttcccct atctcccgat tccggcacat cggcgtcgcg accgggcccg
     6421 gcgaaaagtc gccaaactct tcggtcagat aatggcggaa cgtcggcgga ccgaagccca
     6481 gcctgaagat ttcatgcaga ctctcatgaa tgcccgttac aaggatggcc acgccccgag
     6541 cgacgacgag attaccggaa tcctgctgac ggtgttgttc gcgggccagc acaccagcgc
     6601 agtgctcgca acctggactc ttctggaact tatgaggacg ccgtcgtatc tggcgcgggt
     6661 gaaagacgaa gtgcaagaca cctaccgcga ggcggaaact gtgagctacg agagcctcag
     6721 gcaccaagtc acactggagc acgccatccg tgaatgcgag cggatgcacc cgccgttgat
     6781 catgttgatc cgcaaagtgc tcagaccctt gcattacaag gaccatgtcg tgccggccgg
     6841 tgctttggcc atggtatcgc cggcggtctc tcagcggttg ccccatctat ttgccgaccc
     6901 gaaccgcttc aatccggatc ggttcgcccc acctgcatcc gagggcaaac agcaccacta
     6961 tgccctgatc agttttggcg gcggcaaaca tcgatgcatc ggtgagcatt ttgcttacat
     7021 gcagttgaaa gcaatctgga ccgtgctatt tgatcgtttc gattttgagc ccatcaccga
     7081 gtttcccgaa ccgaattacg gcagttgggt gacaggcccc agggaaccct gccgagtccg
     7141 ttaccgccgc cgcccacagg ccagcatttt ccagtgagca tccccccctc tcggcattac
     7201 gacgtcgcga ttgtcggcgg ggggccagtc ggcagtgtgt gtgcgctcgc ccacgcgcga
     7261 aaaggcgccc gggttgtcct gctcgaagcg agccccgagg cttccaagcg actagcggga
     7321 gaatggctgc acccccccgc cgtgcggatc ctgcgcgaga tcgggatcgg gctcgacacg
     7381 caacctccca tcggtgcagg caacgggttc gtggtgtatc cggaagacga ctcggaaccg
     7441 atcatacttc cctatccaga cggcgctcac ggattggcct gcgagcatgc ggtactcgtc
     7501 tcgcatctgc acgaagcgat cgagaacgaa tcaagcattg actttatttt caatgcacaa
     7561 gtccgtgagg tcgtagacgg acgaatcact tttactcgca acggggccga tgagtccgtc
     7621 gcggccgcac ggatcgtcgg cgccgacggc cggggttcgg tcgtgcggcg atcactgggc
     7681 ttgccgacaa accagaaaac ctgctcgtgg atggtgggag tcctagtcga cgacgtgacg
     7741 ttgccgctgg aaggctatgg atacgtgttg gggggcggac ccgggccgat ccttatgtat
     7801 cgcttgggag agcgtggcgt acgcatcgtc gttgatgtcc cggcggatcg ctggacgccc
     7861 cgggatcgaa tcgggttcct gctggactcg tataccagtt ggttgccgga atcagtccga
     7921 cccgcgtttg ttgacgcatt gcaggcggga cagttccgta ctgccagcaa taaattgagg
     7981 ctgcgtgtca cctacggaaa cccgcatcgg gtgttgattg gcgatgcggc cgggcattat
     8041 caccccatgt cggcggttgg aatcaccctt ggtttcggtg atgcgcttgc actcgccgag
     8101 agcggggact ttcgagactt caccaagaag cgcttccaag cgatccgcgc gccagagttt
     8161 ctcgccatgg gactctacga ggtctttgcc gatcatcggg ccgaggtggt ggctctccgg
     8221 catgcaacct accgaggctg gcgggcaagt tctacgatgc gcgaccggac catgcgttta
     8281 ctcgcctgtg aaaacacatc ggtcattcac ctgagttctg catttttgac gacgatgacc
     8341 cgggcggttg ccggagaaat accgcgatct ttcgacaaac ttgcctggcg ccgtgcccga
     8401 gacaccttta attcactctc gggccgtctc aaatggctgc tgcggggtgc ccgaatgttg
     8461 caccgggtga gaagcacggg tgaaggagag gacgaacaaa tccgggacac ctggtcccac
     8521 acgttcttgg tttccatccc gtcgaagacc gacgactctc aacctgcccg tccatggacg
     8581 atcgaatcgc ccgatgccgg cccggcattg aaaagtgcaa gcaagcgcct gctggatctc
     8641 caggatgggg ttggcgcctg ggaaggcgag atggtctggt gccccatgct caccgcgcag
     8701 tacgtacttc tgcactacat catggggcag ccgctcgacc ccgaccggcg ccgacgcgtg
     8761 ctgcggtctt tcgaacagac tcgcctagaa ggaggcgcat ggggcctgca cgagcaccca
     8821 ccccccaact tgtttgttac cacgctggtt tacgtcgcgg caaggctgct tggcgtcgag
     8881 cgggatgact cactgcttaa gccggcccag gaattcctgc agaccgaggg agttctcggc
     8941 attccgagct ggggaaaatt ctggctggcg ctcctcaatc tctacgactg gcgcggaatc
     9001 aacgccatcc tgccggaact atggagcctg ccgcgctgga ttccgctgca cccctccaac
     9061 tggtattgcc atacccggct catctacatg gcgatggcgg ccgtctactc acatcggttt
     9121 caggcacccg tcacaccctt gatcgcgtca ctgcgagaag aactgtatcc cgagggcttt
     9181 gccaacgtcg gcttttccgc gggccgcaat cgcctgcgaa acgccgacct ccacgcacga
     9241 cccagtgtat ggctccggat cgggtacggg ttttcgcagt tgtttgaacg attccatagt
     9301 aaatacctgc gtgctcgatg catggacgca ataaccgaac gcatccaatg ggaactgcga
     9361 acgaccaatc acacgagcat ctccccggtc agcgggcttc tcaatatcct ggccttatgg
     9421 ctgcgcgacc caaatgatcc cgacagtcgc caggcactcg cgaagttgga cgactggatc
     9481 tgggaagacg aggaatacgg ggcacgagtc accggggcga gaagcatctc atgggatacc
     9541 ggcttcgccc tgcaggcgtt ggaagcgatg tccagtctcg acctagctcc tgaagttcga
     9601 aactccctcc aacgcggggc cgacttcctg gaaacgcagc aaatcaaggc cagtttcgaa
     9661 gggttccgtg aggcttttcg aaacgacccc aaagggggct ggtgctttgc cgggcagtgg
     9721 catggctggc cggtcaccga ctgcactgcg gaagcggtac tcgggattat cgccgcccac
     9781 cgcgaggcga cgaacacggc catgctcaag gatgcgatcc agttcatgct gcgaggccag
     9841 aaccgcgatg gcggctttgg cagctatgaa gcgcagcgtt ccgtaatcgg gctcgaatgg
     9901 ctgaatccgg ccgagatgtt cggcgagtcg atgaccgaac attcctatgt cgagtgcacc
     9961 gcttcttgcc tcgcagcact cgctgcctgc aagaagcatt ttccggacac cacggaccaa
    10021 gcagtgacgg atgccatgtc gcgagccagc gcctggcttc gccgaaccca agcaagcgat
    10081 gggtcgtggc gcggcgtgtg gggggttcag ttcatctacg gcacttcctt cgggatcaga
    10141 ggcctgcttg cagctggagc ccgtcccggc gatccggcac tacgcctagc ctgccggtgg
    10201 ctgctggacc gtcagcgcgg agacggagga tggggtgaac actacagcgg ttgcttcacc
    10261 ggccagtaca tcgcgcacga tgaaagccaa gtagtccaga ccgcatgggc attgattgcg
    10321 ctccttgagg cggacgatcc caactgggcc gcgatctcgc ggggtgcccg gttcctgctc
    10381 gacacccaga acgcagatgg cgcatggccc aagcaagaca tggccggggt cttcttccgc
    10441 acggccctgc tggactacgt cctgtatcgg cagtatttcc ctctgcacgc gctcgctctc
    10501 tacgagcagc ggcgtcggac ccggttggaa ttggcagcca tcgattccct taccgccgaa
    10561 acgaccgcaa acagcgaaac acctttggct gcggctacat tagaagaacc acacagcgcg
    10621 cacgtccctt cttgaagaaa agctcggttg gagaaaaatt gaatgaggct tacacctgct
    10681 aaacgatcag attctcgttc cctcgcggac ctaactccgg atgaggtgat acaggtagtc
    10741 aatcactacg ccgggctcca cgatgctgac ttggaggata gaaaagaaga ctaccaatcg
    10801 cttgtcaatc actactacga cctagtgact gacttctacg agttcggctg gggccagtcc
    10861 tttcactttg caccccggca tcggggggag agtttcaaag aatcgttact caggcacgag
    10921 catttcctag ccgaccaact gtccctgaag ccagggatgc aagtacttga tgtgggttgc
    10981 gggatcggcg gccctatgcg cgttctggcg cattattccg gggccagttt tgtcggcatt
    11041 aacaataacg cttaccagat cgaacgagcg aaagtgcaca ccgaagatgt ccggtcattg
    11101 tgccgcttca tccgcggtga ttacatgcag attccggaag atgataattg ttacgatgcg
    11161 gccttcgcca ttgaatccat gccccacgca ccaaacaaga ccgcagcgtt ccgggaaatc
    11221 ctccgcatcc tgcgcccagg atcttgtttt acaggctacg aatggtgtct taccgacgcc
    11281 ttcgatccgg agaataccga gcaccttcga atcaagaaag tgatcgagag agggaatgga
    11341 ctgccggata tcgctgccac atcggaagtt tgcgatgcat tgcgcatggc cggattcgag
    11401 attctggacg cccgcgacct cgcccctgaa tcgcatcccg aaacaccttg gtatcgagct
    11461 ttgcagggtc gcgatctcag cctcgcaagt gttccacgta caccgctcgg gcgtgccttg
    11521 accaatttga tattaagggt cggggagaaa ctgcgggtat ttccggaggg aaccaggatg
    11581 gtcagtacgc tgttgaaccg gggtgcggac gcattggttg aaggaggcga aaccggcatc
    11641 tttactccga tgtttttctt cctagcccgc aaacccaagc attcaggaac tgaataagga
    11701 atatttctga cccgagtccc ctgcaaatca tccaccccgt cgcaggctct tgccggtagt
    11761 cgttccagag gattttagaa atttgacaaa atagccggat ggcactgcaa ccggctccac
    11821 ttttgacttc acccacgccg cagcagcagc acactcccag catgcgccag taccttgagt
    11881 tcaaggcgca atatcctcag cacctgctgc tctacagaat gggagacttc tacgagttgt
    11941 tcttcgagga tgccaaacgc ggctccgaat tgctggatat cgtgctgacc gagcgggggc
    12001 gctccaaggg ccagcctatc ccgatggcgg gcatcccggt ggtttcgctt gattcctatc
    12061 tcgcaaaact tgtcgacctc ggagaatccg cggtggtttg cgaacaggtc ggcgacagcc
    12121 cgtccggccg agggccgatg gaacgccaga tctcgcgcat cgtcacgccc ggaacgctga
    12181 tcgaagacga actgctggac gccggacgcg acacgctgct gcttgcgctg tggccgggag
    12241 atccgatcgg gctggccagc ctgaacctga gttcgggacg cttcaatgta atggaacttc
    12301 catccatcga agacctgtac gcggaactgg aacgtctccg tccggcggaa atcctgtatc
    12361 cggaatcgaa cacccctccg gactgggaag acacgcattg gcagcctctt tcagactggc
    12421 atttcgacgt tgaaaccgcc acgcgactct tgtgccaaca actcggggtc catgacctgt
    12481 ccgcctttga ctgccaagac gcaccctccg ccaccggagc cgccggagcc ctgctctact
    12541 acgcccaaga gacgcaacgg gcagccctgc cgcacattac gagcctgcag agggagaggc
    12601 gcgataccca catctacctg gatgccacca gccgacgtaa tctcgaactg gagcagacca
    12661 tctccggcga ctcccgccac agcttgctag gcgttttgga caaaaccgtc acggccatgg
    12721 gccgccgttg ccttagccga tggatccagt ctccccttcg cgaccgggag acgctggcga
    12781 cacgccacca aatcgtttct cacctgttgg acaacacgca ccgcgccccg attcgcgaac
    12841 agttgaggaa agtctccgat atcgagcgcg tcatcgcccg ccttgcgctg tataccgctt
    12901 ctccgcggga cttatgcggc ttggctcaaa ccctgcagcg cctgccgaac ctgcgccgcc
    12961 tgctggagaa catcgatgca gcggaattcg attcctaccg cacccatctg cagtcggacc
    13021 cggaactggc tacacatctg gcctcagcgc tcgcggaaaa tcctcctccg accattcgcg
    13081 aaggtggcgc cattgcggcg gggttcgatt ccgagttgga tgaactgcgc gaactgtcgc
    13141 gcggagccga cgattttctt ttggagatgg aagcaaccga acgggagagc accggcatcg
    13201 cccagctcaa agtcgcctac aaccgggttc acggctacta catcgaggtc ccgcgcagcc
    13261 aagccgcgcg ggtgcccgat cactaccggc ggcggcagac gctcaagaac tacgaacgct
    13321 acctgacatc ggaactgaag gaattcgagg atcgcgttct gagcgcgcgc agccgggcgc
    13381 tggagcgaga gaaacaggtc taccagaatc tcctggaaag cctgacgcca cacctgaacc
    13441 acttcaagca atgtgcagcc gcactgtccg aactagacgt gctctgcagc ttcgccgagc
    13501 aggccaaggc gcaccactac tgctgccccg aactggtcga cgaacctggc gtcgagatcg
    13561 aagctggtcg ccacccggta atcgaactga cttggaacga accattcgtg ccaaacgaca
    13621 cgctgctttc cgagcaacgc cgcctgctcc tcgtcaccgg cccgaacatg ggcggcaaat
    13681 ccacctatat gaggcagacc gcactgatcg tgctgatggc ctgcatcggc agccatgtgc
    13741 cggccaagca ggcccggatc ggccccatcg accgcatctt cacccgcatc ggggcctccg
    13801 acgacctgac ttctggccgc tccacgttca tggtcgagat gacggaagcc gccaacatcc
    13861 tgcaccacgc ctccgaatac agcctggtgt tgatggacga gatcggtcgc ggcaccagca
    13921 ctttcgacgg gatggcactg gcactgggct gtgccgaata cctggccacc caaaaccggg
    13981 ccttcaccct tttctccacg cactacttcg agctgaccca actggccgag atccaccccg
    14041 ggatcgtcaa ccttcatcta gacgccacga tgcatggtca gcagatcgtc ttcctgcacg
    14101 ccgtcaagga aggacccgcc agtcagagct acggcctgca ggtcgcacgt ctcgcgggca
    14161 ttcctgcgcc ggtgctggaa cgggctcagg catacctgct ggaactcgag gacgacagca
    14221 cccaacgctg ggcacgcagc aatccgcaaa aagacctgtt cgtgccgcct tccgatcctc
    14281 catccgtccc ggccgcctta gaacgcctcc gacaggtgaa gcctgaccaa ctgtcgccga
    14341 aggaggcctt agacctgatt tatgaattgc agaaact
//
