LOCUS       BGC0002450             14982 bp    DNA     linear   ENV 16-MAR-2020
DEFINITION  Uncultured bacterium glycosidation gene cluster, complete sequence.
ACCESSION   BGC0002450
VERSION     BGC0002450
KEYWORDS    .
SOURCE      uncultured bacterium
  ORGANISM  uncultured bacterium
            Bacteria; environmental samples.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:44:08
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..14982
                     /collection_date="29-Jun-2015"
                     /db_xref="taxon:77133"
                     /environmental_sample=""
                     /host="Melophlus sp."
                     /isolation_source="GUM22 sponge holobiont tissue"
                     /lat_lon="13.4513 N 144.656 E"
                     /mol_type="genomic DNA"
                     /organism="uncultured bacterium"
     subregion       1..14982
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002450"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14982
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            365..838
                     /gene="mgb1"
                     /locus_tag="mgb_00001"
     CDS             365..838
                     /codon_start=1
                     /gene="mgb1"
                     /locus_tag="mgb_00001"
                     /product="hypothetical protein"
                     /protein_id="QIJ55914.1"
                     /transl_table=11
                     /translation="MQAVRQQRLQAIPHPLPPAHQAGRVQRQLMLQVLVAAEVLPVRVL
                     QPARHHALVALVERVLQIVQPHQQTRRQRRLPRPRTVLHVDLRFQAQPVHLPGQAHQRV
                     AEVEDLRQLGAEQVRGMIGLFRHHGLQGIGENPLFPCARSYQKSTFKSIKSIC"
     misc_feature    365..14922
                     /note="glycosidation gene cluster mgb"
     gene            complement(1335..1850)
                     /gene="mgb2"
                     /locus_tag="mgb_00002"
     CDS             complement(1335..1850)
                     /codon_start=1
                     /gene="mgb2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) dTDP_sugar_isom"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="mgb_00002"
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="QIJ55915.1"
                     /sec_met_domain="dTDP_sugar_isom (E-value: 1.5e-53,
                     bitscore: 171.0, seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRFLKTDLPGVVRVIPEPHEDLRGSFARVYCSMEFAEAGLGDFTP
                     VQINLSRNPEQHTLRGMHWQDPPYAEAKLIRVTRDAAWDVAVDLRPESPTRLRWVAACL
                     DAESAEALFIPEGFAHGFLTLEPDTDVLYQMGRTHEPGHECGARWEDPAFSIDWPAHPV
                     VISDRDRA"
     gene            1985..2581
                     /gene="mgb3"
                     /locus_tag="mgb_00003"
     CDS             1985..2581
                     /codon_start=1
                     /gene="mgb3"
                     /locus_tag="mgb_00003"
                     /product="hypothetical protein"
                     /protein_id="QIJ55916.1"
                     /transl_table=11
                     /translation="MPWRVDGNMAAGQQHALFLRVSIHSKSDAVGANTCDIEKSITLCR
                     GTVGSDCLPFVYHFIDECPQISADLFYPLTKGGVRVDSIQFHARLIHTNFGQAGLYRMH
                     IRMSHKNTQGSSMSRQHLNVKHLKTYGLHQLFHEQEQKIRIMLMVYRVELAESNEIGQM
                     RKFESSNARRLQQYHKTGNEITEIGHMSQDIVRHH"
     gene            complement(3073..4158)
                     /gene="mgb4"
                     /locus_tag="mgb_00004"
     CDS             complement(3073..4158)
                     /NRPS_PKS="Domain: NAD_binding_4 (85-202). E-value: 2e-07.
                     Score: 22.2. Matches aSDomain:
                     nrpspksdomains_mgb_00004_NAD_binding_4.1"
                     /codon_start=1
                     /gene="mgb4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     167.4; E-value: 8.3e-51)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="mgb_00004"
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="QIJ55917.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.1e-14, bitscore:
                     43.3, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 5.1e-14,
                     bitscore: 42.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEGLVVMPEFWEGKRVLITGHTGFKGVWATRLLVHLGAEVHGLAL
                     PPDSDPSLFAMLGEEGLTSSTLGDMTDHEAVAHTVSEVQPQFVLHMAAQPLVRRSYREP
                     VETFATNVMGTVHLLDSLREQDGVEAVLVVTTDKVYENKQDEGAFTESDRLGGYDPYAA
                     SKVAVEVATAAFDLSYFSANGVPLATARAGNVIGGGDFSEDRLIPDIVRAEMEGVPLKL
                     RNPQATRPWQHVLDCLAGYFFYIQALSEGRDMPHSLNFGPTSPEDNLTVAQMQETFSSI
                     FGADQNWERDVAAAPREMDTLAINADMAQRLLGWNSCYSPLQAIRLTAEWYRAWRDGAD
                     PLSLLDAQIASFVKLVGKFTR"
     aSDomain        complement(3553..3903)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_mgb_00004_NAD_binding_4.1"
                     /evalue="2.00E-07"
                     /label="mgb_00004_NAD_binding_4.1"
                     /locus_tag="mgb_00004"
                     /protein_end="202"
                     /protein_start="85"
                     /score="22.2"
                     /tool="antismash"
                     /translation="QFVLHMAAQPLVRRSYREPVETFATNVMGTVHLLDSLREQDGVEA
                     VLVVTTDKVYENKQDEGAFTESDRLGGYDPYAASKVAVEVATAAFDLSYFSANGVPLAT
                     ARAGNVIGGGDFS"
     gene            complement(4140..4910)
                     /gene="mgb5"
                     /locus_tag="mgb_00005"
     CDS             complement(4140..4910)
                     /codon_start=1
                     /gene="mgb5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 146.3; E-value:
                     1.8e-44)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="mgb_00005"
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="QIJ55918.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 2.1e-10, bitscore:
                     31.2, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQVVILAGGFGTRISEESHLRPKPMIEIGGYPILWHIMKIFSHHG
                     LNEFVICLGYKGYVIKEYFANYVLHSSDVTLDATTGEMIYHQSVAEPWKVTLIDTGRDT
                     MTGGRLKRVAPYLKKQFCMTYGDGVADVDISALIRFHESHGCEATVTSVMPPGRYGTLE
                     VRPDNCVERFVEKPSGDLGLINGGFFVLNRSVIDRIEGDDTPWEADPLTSLAADGQLMS
                     WRHHGFWQAMDTLRDRNHLEEIWTGGNAPWKVWS"
     gene            complement(5226..6095)
                     /gene="mgb6"
                     /locus_tag="mgb_00006"
     CDS             complement(5226..6095)
                     /codon_start=1
                     /gene="mgb6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     133.5; E-value: 1.8e-40)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="mgb_00006"
                     /product="GDP-6-deoxy-D-mannose reductase"
                     /protein_id="QIJ55919.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 6.6e-11, bitscore:
                     31.8, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRVLITGMQGFIGRHVSAGLRSRGADVIDARRSGACELLREDGR
                     TQAMDGIHADTLVHLAWVTAHGENWTSDLNRDWEIATADLIARFFARGGRRVIATGSCA
                     EYDWTQGGVLSENAPLVPHTPYGAAKARTGEALLKAADDHGASAAWARVFFLFGNGEPS
                     ARLVPSMLDACLKRKALACGPHNTVRDFWDVRNLGDALAALALSDLSGPVNVASGRGVS
                     FSELGELIEKITGVDGMIHFDERPLGEGEPPTIVADTARLRSELDFTESIPLETGLLDY
                     RNALAGTI"
     gene            complement(6121..6993)
                     /gene="mgb7"
                     /locus_tag="mgb_00007"
     CDS             complement(6121..6993)
                     /codon_start=1
                     /gene="mgb7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="mgb_00007"
                     /product="glycosyltransferase"
                     /protein_id="QIJ55920.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 7.8e-25,
                     bitscore: 77.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVHNGASLLRESLECLRAQDYEDFEVVISDNASTDETEEICTEF
                     VERDSRFRYLRHSENIGLLANFRGLLDMTEAPLFMWRAYDDLSDSNYISELVSLFDQDS
                     GTRLAVPKIRLLNISGAPDENWECRLHPYQSVSGGRSKVLDIAQEMFHSPPNWSYGLWD
                     RAYLADEQSRVNAAYPYPWASDDLVLFPPILDRAIRGSNSTCFTQRFTSRSHQLPETRV
                     LRDLRVKFVRKCRSDIARRKWSPSEWILLQTLLPRYCDVRTQTHSRASRLKPVRRLLLR
                     RLCNKLSSS"
     gene            7298..8290
                     /gene="mgb8"
                     /locus_tag="mgb_00008"
     CDS             7298..8290
                     /codon_start=1
                     /gene="mgb8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="mgb_00008"
                     /product="glycosyltransferasein"
                     /protein_id="QIJ55921.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.6e-10,
                     bitscore: 30.5, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKIAVGVATRNRPRMFAHMLESFLNLKWPDDSQIEFIFLENNHT
                     LQIQNTVEDFKEKLFKNTGNIPHIHTTAVESRHGIPFVRNRILDIALDKGCDYLAFCDD
                     DQTVNSDYLVELVRAAKVRSLDLVGSIARVETSSSASDLTFFQKLVYNELRSHQEKQES
                     HAIRLHTAHKDQDWKPGSGAMLCRLDFLRQHRIRFNESLRYSHCEDLDFYYQVKTAGGQ
                     TGCAPRAIVYEVMPESRLSPTFQFKRHKNLTLARQNIRYAWGVKEKKVIRLSCTILGKL
                     ILGISRICLAPFNRGRSFTRGVRVLGGALGRLQSLLGHEDKHYVQTDGN"
     gene            8508..9458
                     /gene="mgb9"
                     /locus_tag="mgb_00009"
     CDS             8508..9458
                     /codon_start=1
                     /gene="mgb9"
                     /locus_tag="mgb_00009"
                     /product="glycosyltransferase"
                     /protein_id="QIJ55922.1"
                     /transl_table=11
                     /translation="MDTKRPIIVSAADDAYMEFLDDMLASIQNRLHEYDLGILDLGLSK
                     RSLAMIRARKEDARVVDPGWRYQPADMSKQPPYKKVYYAKPYLPDIFPGHVGYMWIDAD
                     VWFQDSSAIEHYISASEKTPGRGSIAFECHPNYKDINYKPRFRMVRKYRILPVAIRLVG
                     RKTFRLVYDMFGDEAARTHGLAPDNNSGLFFIHADSPAWKAWQHYMRKANHLPFMDRKY
                     FPDQTCLNVALRERNISFHVMQPIYNWIVGLGLPMLDENTHALLDPCSPHKKIYAIHLA
                     GSAGFQPSNLRTTSGNHASVQLKMSKFYSETTANQ"
     gene            9616..10359
                     /gene="mgb10"
                     /locus_tag="mgb_00010"
     CDS             9616..10359
                     /codon_start=1
                     /gene="mgb10"
                     /locus_tag="mgb_00010"
                     /product="ubiquinone biosynthesis O-methyltransferase"
                     /protein_id="QIJ55923.1"
                     /transl_table=11
                     /translation="MLEMVIECLEDGPLHILDIGCGSGALLQRIADYYRAQGWNPDEYL
                     LGTDMDEDFFQAKVPFQVSELTKPLNFQMRSFDLIITMEVLEHVRSPYTLLEEIYATLN
                     PDGKLLFSVPNMMTIVSRFRFLFTGRFRHYDGPSSDPADADSGMGHINPFPIQYWDYGF
                     RYAGFTDIRYRTDRIKRGALYFSAFLSPLMWLGKHWLHRQERNKNLRVYQQNLRPFREI
                     NSLRNLAGHGLVATCTKSGNPVSEK"
     gene            10377..10844
                     /gene="mgb11"
                     /locus_tag="mgb_00011"
     CDS             10377..10844
                     /codon_start=1
                     /gene="mgb11"
                     /locus_tag="mgb_00011"
                     /product="hypothetical protein"
                     /protein_id="QIJ55924.1"
                     /transl_table=11
                     /translation="MVVIRGRVIKGKGGYGKASHSSEVAAYFQNTPVRGTLNIALDQPV
                     RLRHAKALISMIWPSYIGGIPCLITRTSHTPLHIVEVISDEQLPCTRGDRVTLVIPRGC
                     TVDLPPIHHTIWKVFYGYGRTHWYEYKIYRRLLGPFYFVRRLSIQDPKRPD"
     gene            complement(10858..11193)
                     /gene="mgb12"
                     /locus_tag="mgb_00012"
     CDS             complement(10858..11193)
                     /codon_start=1
                     /gene="mgb12"
                     /locus_tag="mgb_00012"
                     /product="methyltransferase domain containing protein"
                     /protein_id="QIJ55925.1"
                     /transl_table=11
                     /translation="MPVAGNLSLIFDMSRLIKDKAARGQHLLEEAVLEVLLEARKNGDG
                     LVRAIDIARRVDIYRKKFTPSMGNAITSGILAKLSEEKRIEQLRRRGPWKLTEEEFSKM
                     SADQNDA"
     gene            complement(11218..12798)
                     /gene="mgb13"
                     /locus_tag="mgb_00013"
     CDS             complement(11218..12798)
                     /codon_start=1
                     /gene="mgb13"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 147.5;
                     E-value: 1e-44)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="mgb_00013"
                     /product="GDP-mannose-dependent
                     alpha-(1-2)-phosphatidylinositol mannosyltransferase"
                     /protein_id="QIJ55926.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 8.4e-33,
                     bitscore: 103.5, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSILVTLRGCVISGRGTASKIRAFGPEVINFFGEPPVRGSLNLAL
                     EKPVRFDPEQVELPGGKRTFFWACEIKGMRCLITRAKGHPLHIIEIVAPCKLRDKFQLR
                     DGDSMELKVSQEIVLEDLSWGTKAAWNFFWRFRECWYVSKAYRMLIGPFWLIRRMATQS
                     PQYHAKLWNRKKLRDDISVCHINLARNPKLRGGERQTEILVEALAAEGVSRQRVVVLRQ
                     GPLAQRFHSRPNLEVCRVRNRLSAIFVCRGASLLHAHEAHAAQVAYAASLFGKQYLITR
                     RLTKPVHSNSYSSAVYRNAQTVVALTEAVENSLRDRFPDASIDRIPDAWNPEYPDPDGV
                     RQIKKKFAGKFVVGHAAAMDGPEKGHSVLLQAARTLQQDAPDFQFVLLGSGRLEEEFRQ
                     QAEGLTNVHFAGWVEDPITWIAAFDLFAFPSLVEPLGSTLLDVMRAGVPVVASRVDGIP
                     EIITEGCGALVPPGDAGALAEQLLRLSRSEELRQRLSEKGLERVKEFSPELIAREHMKI
                     YQDHGLVLD"
     gene            complement(12817..13233)
                     /locus_tag="mgb_00014"
     CDS             complement(12817..13233)
                     /codon_start=1
                     /locus_tag="mgb_00014"
                     /product="hypothetical protein"
                     /protein_id="QIJ55927.1"
                     /transl_table=11
                     /translation="MEIWLEQTIPLLSIAVPLIAGIFGWWYNERKKFQWEKHKRKEDRY
                     RGFIESIQGFYVLSQDKGEKEKFIKELRLAWLYCPDEVIQAGNAFLDTVSTGVESSAEQ
                     KEQALAEFKIALRRDLYGKTRLTVGDHRIWSAQS"
     gene            13358..13777
                     /locus_tag="mgb_00015"
     CDS             13358..13777
                     /codon_start=1
                     /locus_tag="mgb_00015"
                     /product="hypothetical protein"
                     /protein_id="QIJ55928.1"
                     /transl_table=11
                     /translation="MEFLKPRTLKARQKTLALLLPCLTPVQDDLGNVPVSMQQDPHVNG
                     ALIGLTERVCAHFGVTRQALVHRVTAAVFEEIYRREATAVLTRCDEFLEDPQSELSRAR
                     ANIGELPSETPDPNWVSDLNGYIQKNYERPDILVL"
     gene            13789..14922
                     /locus_tag="mgb_00016"
     CDS             13789..14922
                     /codon_start=1
                     /locus_tag="mgb_00016"
                     /product="hypothetical protein"
                     /protein_id="QIJ55929.1"
                     /transl_table=11
                     /translation="MDIALHRSASDIEEDLEYYFSEQRDKHNQIRPSDDIRSFTTDSPN
                     FTEDPGENGIGNVFHPFAPFSLGRIFIGISFPLFSNNWGAAFLRHLLGLIKPKGAVILP
                     VYPEIQARDQGLWCRSSLENIFRSRSRYIGISNIWAENDGVMSMRVGRRWPPIIPSTAR
                     WLFEQTPRRAVALGLEDNTEAIQKFWQSEVQRFWRLGRYHAVIEQIIRNQYGPRRPVRL
                     ATTGEDAGPLALECLYSPYTRITRADVYGPNLSPDLLEALDQACLTQEHGPLECPGTYD
                     TTNSFDVLCVSGPERLSPDSVGRILTTNGILILTPECAALNPELGPGFKEPEYYSDTVA
                     QRLHPSIPIYHYSERIEEEIAQQTESHQSAFMVLSKA"
ORIGIN
        1 ccggcaaccg ccgggccgac tccagaagcg ccggcaacac cggatcgtcc gccgccatct
       61 tgcgttccac gtcccgcacc acccggccca gccaggtctt cagcttgcgc gtctcccgcg
      121 ccgaacgccg catctgccgc gcccgcgcgt agcgcccctg catcaccagc gccaccttgc
      181 cgacccgccg gcagctctgc cgcagcacga tgccggagtc ctgcgccgcc cgcaccaatg
      241 ccacccgcat cttctggtac aggcgcgcat cggtcgggaa cgccaccgcc ttctcctgca
      301 ccgtcgtgtc cacgttcacc cgcgccagct cgcgcaactt cagcaagcct tcccgccgcg
      361 ccacgtgcag gctgtgcgcc agcagcgcct ccaggcgatc ccccatccgc tcccgccagc
      421 gcaccaggcg ggtcgggtgc agcggcagct catgctgcag gtactcgtag ccgcagaagt
      481 actgccagta cgggttctcc agccagcgcg ccaccacgct ctcgtcgctc tcgtcgaacg
      541 cgtacttcag atagtgcagc cccaccagca gacgcgtcgg cagcggcggc tgccccggcc
      601 ccggactgta ctgcacgtcg atctccgctt ccaggcgcag ccagtccatc ttcccggaca
      661 ggcgcaccag cgggtggcgg aggttgagga tctgcgacaa ctgggcgcgg aacaggtccg
      721 gggcatgatc gggctttttc ggcatcatgg tttgcaggga atcggagaaa acccgctgtt
      781 tccttgcgcc cgatcctacc aaaagtccac ctttaagtca ataaaatcaa tctgttagga
      841 atacttcagg ggcgactact tacccgatcc acttcccaga ttcgagacgg aggaaactgg
      901 tggagatgaa tgatttggca gatttccgat tgacattgac cctgtatctt cgaattaatg
      961 ctggaggtga agcgattgga gtcactagat tctccatatc gtgctgtatt gaatctcagt
     1021 gaatacaact caatcagcaa cataacaaaa cccaaaaccc aacatttcaa tcccctatgc
     1081 cttgccgggc cggcattacc ataaaccttg gccgactgca gcgggaatgg aagcgaatgc
     1141 accctaccct gtgccactgg aaagttcttg gttgctacaa cagtaaatct gaagcccaag
     1201 cccaagaaaa cgtgctggca caacgatatg gatgcacggc atctcctagc ggtgatgggg
     1261 aagaagtcac gaagcggtat gctattactt ggagccttga cccttaggcc gccaccctac
     1321 cgcgtaaaat caggtcaggc tctatcacgg tcagaaatca caaccgggtg agcaggccaa
     1381 tcaatcgaaa aagccggatc ttcccaacgt gcgccacatt cgtgaccagg ttcatgcgta
     1441 cggcccatct ggtaaaggac atcagtgtca ggctcaagcg ttagaaatcc gtgggcgaaa
     1501 ccttcgggaa tgaataatgc ttctgcgctt tcggcatcaa gacatgcggc aacccaacgc
     1561 agtctagtag gactttcagg cctcaaatcg accgctacat cccaagccgc atcgcgtgtg
     1621 acacgtatta gtttggcttc cgcataaggt gggtcctgcc aatgcattcc tcgcagggta
     1681 tgttgctcgg gattgcgtga gagattgatt tgtacaggtg tgaaatcacc aagccctgct
     1741 tctgcaaatt ccatggagca ataaacccgg gcaaaggatc cgcgcaaatc ttcatgtggt
     1801 tccgggatga cccgcacgac gccaggcaag tcagttttca ggaaacgcat cagaacacgc
     1861 gaaagtcagg tatgacggtt acgaactttc caccccaacc ccggatggca acctgctggc
     1921 tcgcaatttc atcagcaaga ttccagggca ggattaggag gtagtcgggt ttttccgcag
     1981 gaatatgcct tggcgcgtag acgggaatat ggctgccggg cagcagcatg ccttgtttct
     2041 ccgggtttcg atccacagta aaagcgatgc ggtcggcgcc aatacctgcg acattgagaa
     2101 aagtattacc ctttgccggg gcaccgtagg cagcgattgt cttcccttcg tctatcactt
     2161 tatcgacgaa tgtccgcaaa ttagtgcaga tctgttctac ccgctcacca aaggcggcgt
     2221 aagggttgat agtatccaat tccatgcccg cctcatccat acgaactttg gccaagccgg
     2281 gctttatcgg atgcacatca ggatgtccca caaaaacacg cagggatcct ccatgagtcg
     2341 gcagcatctc aacgtcaaac acctcaagac ctacggcctg catcagttgt tccacgaaca
     2401 agagcagaag ataagaataa tgctcatggt atatcgtgtc gaactggccg aatcgaatga
     2461 gattggccaa atgcggaaat tcgaaagtag caacgcccga aggcttcagc aataccacaa
     2521 aaccggcaac gaaatcacgg agatcgggca catgagccaa gacattgttc gccaccatta
     2581 gatcggccgc agaacccttt tcacgcaact ccctcgctgt ctgctcacca aaaaaagcaa
     2641 cgtgagtcgc tacaccctga ctttctgcaa tctccgccgc atggcctgcc ggttctaccc
     2701 ctagcacggg aatatccgat cgagcaaacc actgcaacag atagccatca ttcgacgcca
     2761 cctccaccac acgactgcca gcatcgagtc caaaacgttc tgtcatcgtc tgagcgtaac
     2821 tttgtgcatg tgccacccat tcgtttgaat aggacgatag gtaggcataa tcatgggtaa
     2881 aaatctcatc tgctggtatt gtttctgtag tctgaaccaa ccagcacgaa tcgcatacca
     2941 tcacacgcaa agggaaagtc cgctcggcct cacaagcctc tggggttggg ggcagatagg
     3001 aattagcaag tggagtctgt ccgaagtcca caagctcgtg tagcaacggc gccgcacaga
     3061 accgacaaac tgtcatcggg taaattttcc gacaagcttg acgaaggacg caatctgggc
     3121 atccagcaaa gacagcgggt cagccccatc acgccatgct cgataccatt cagcagtcag
     3181 acgaattgcc tgaagtggcg aatagcaact gttccagcct agcaaacgct gtgccatatc
     3241 cgcattgatg gcaagcgtat ccatttctcg cggagcagcc gccacatctc gctcccaatt
     3301 ctggtcagct ccaaatatag aagaaaaagt ctcctgcatc tgagccacag tcaagttgtc
     3361 ttcaggactg gtcggaccaa aattgagtga atgtggcata tcacgtcctt cggataacgc
     3421 ttggatatag aaaaaatagc ctgcaagaca atcaagtaca tgttgccaag gccgcgtagc
     3481 ctgtgggtta cgcaacttga gtggtactcc ttccatctcg gcccgaacta tatcgggtat
     3541 taaccggtct tccgaaaagt caccgccgcc aatcacattg cctgcccttg ctgtggctaa
     3601 cggcactccg ttggcagaga aatatgaaag atcgaaagcc gctgtcgcaa cttcaaccgc
     3661 aaccttggat gcggcataag gatcgtaacc tcccagccga tcactctcgg tgaaagcgcc
     3721 ttcatcctgt ttgttttcgt aaaccttatc cgtcgtgaca accagcaccg cctccactcc
     3781 gtcctgttcg cgcaacgaat caagaaggtg gacagttccc attacgtttg ttgcgaaggt
     3841 ttctactggc tcacggtagg accggcggac aagcggctgt gcggccatgt gcaggacgaa
     3901 ctgcggctga acttctgaca cggtatgagc cacggcctcg tggtcagtca tgtcgcccag
     3961 ggtggaggaa gtcaaaccct cttcgcccag catggcgaaa agagacggat cactgtccgg
     4021 cggcaaggca agtccgtgga cttcggcacc aaggtgaacc aataatcgcg tggcccaaac
     4081 tcctttgaat ccggtatgcc cagtaattag tacccgcttg ccttcccaaa attctggcat
     4141 tacgaccaga ccttccatgg tgcatttcca ccagtccaga tctcttcaag atgattacgg
     4201 tcacgcaagg tgtccattgc ctgccaaaat ccgtggtgtc tccaactcat caactgtcca
     4261 tcggcagcaa gcgaagtcag cggatcagct tcccatggag tgtcgtctcc ttcaattcgg
     4321 tcaataaccg aacggttaag tacaaaaaaa cctccgttga tcaaacccaa atcccctgat
     4381 ggtttctcga cgaaccgctc cacacaattg tctggtcgaa cttcgagcgt gccataacgc
     4441 cctggcggca tcacacttgt caccgtcgcc tcacatccat ggctttcgtg gaaccgtatg
     4501 agggccgaaa tatctacatc cgcaacaccg tcgccgtagg tcatacaaaa ttgttttttc
     4561 aggtatgggg ccactcgctt aagtcgtccc ccagtcatgg tgtcgcgccc ggtgtcaatt
     4621 aacgtcactt tccatggttc agcgacggat tgatggtaaa tcatctcacc tgtggtggca
     4681 tccagtgtga catccgagga atgcaggacg taattcgcga aatactcctt gatgacatag
     4741 cccttgtagc ctaagcaaat tacgaattca ttgagtccgt ggtgcgaaaa aatcttcatg
     4801 atgtgccaca ggatcggata tccgcctatc tctatcattg gcttgggacg cagatggctt
     4861 tcttccgata tgcgcgtacc gaatcccccg gcgagtatca caacctgcat gacttccggt
     4921 tcctgaatag acaaatcatg tgctgggaaa actgcaccaa ttcaaacccc cgcgccatta
     4981 gataattcgt aaccggaacc accgtcattt cttcttcacg cccttcacgc gcgtcacccc
     5041 cgccgccgcg tcatggaacc gccgcagcca gtcatgcacc gcattcgccg aaggaaaggt
     5101 gcgcctgcgc ccgccgcgga agcgcgccgc cagtgccgcc ggcggtgcgc ccagaaggtc
     5161 cggctcattt caagattgga atagagcatg tacaggcaac ttccctttgt catgcagcga
     5221 tgccattata tggtgcccgc caaagcgttg cgataatcca agaggcctgt ttcgagagga
     5281 atcgattcgg tgaagtcgag ttcggagcgc aatcgcgcgg tgtcggcaac aattgtcggt
     5341 ggttctccct caccgagcgg acgctcgtcg aaatggatca tgccatcgac gccagtaatt
     5401 ttctctatca gttcgccgag ttctgaaaac gagacccctc ttccgcttgc aacattcaca
     5461 gggccggata gatcggataa tgcgagagca gccagcgcat cgcccagatt tcttacgtcc
     5521 caaaaatcgc gtacggtatt gtgcgggcca caagccagcg cctttctttt gaggcaggca
     5581 tccagcatcg atggcacgag ccgtgcggac ggctcgccat ttccaaacag aaagaatacg
     5641 cgagcccaag cggcactcgc gccgtggtca tcagccgctt tcaatagggc ttcgccggtt
     5701 cttgctttgg ccgcaccgta cggcgtatgc ggaaccaatg gcgcgttctc cgacaataca
     5761 cctccttgag tccagtcata ttcggcacag gaccccgttg cgattacgcg gcggccacca
     5821 cgagcgaaaa aacgggcaat gaggtctgcc gtcgcaatct cccaatctcg gttgaggtcg
     5881 ctggtccagt tctctccatg cgcggtcacc catgcgagat gcacgagcgt atctgcatgg
     5941 attccgtcca ttgcctgggt acggccatct tcccgcaaga gttcgcatgc gcccgagcgc
     6001 ctggcatcga tcacgtcggc acctcgggac cgaagccccg cagatacatg gcggccaata
     6061 aaaccctgca tccccgtaat caagacccgc ctcattgcaa tcctgtaaat gactaagtgt
     6121 tcaacttgaa ctcagcttgt tgcataatct ccggagcagc aagcggcgca ctggcttcag
     6181 tcgggaagcg cgagaatgag tttgagtccg gacatcgcaa taacgcggta acagggtctg
     6241 aagcagaatc cattcacttg gcgaccattt tcgcctagca atatcagaac ggcatttcct
     6301 cacgaatttc acccgcaaat cccgtaagac acgtgtttct ggaagttgat ggctacgact
     6361 agtaaaccgt tgcgtaaaac aggttgaatt gctaccgcga atcgcccggt ccaaaatcgg
     6421 aggaaacagc accaagtcat ccgaagccca tggataggga taggctgcat tgactcggct
     6481 ctgctcgtcg gccaaatacg cccggtccca aagtccgtag ctccaattcg gaggactgtg
     6541 aaacatttcc tgcgcgatat ccagcacctt gcttctaccc cctgacacag actggtacgg
     6601 atgaagacgg cattcccagt tttcatcagg tgccccggaa atattcagta ggcgaatctt
     6661 gggaacagca agacgtgtcc cggagtcctg gtcgaataag gatacgagtt cggagatgta
     6721 gttggaatcg ctcaagtcat cgtatgcccg ccacatgaaa agcggcgctt cggtcatgtc
     6781 gagcaatccc ctgaaattgg cgagcagacc aatattctct gaatggcgca gataacgaaa
     6841 gcgactgtct cgctcaacga attcagtgca aatttcttct gtttcgtcag ttgacgcgtt
     6901 atcgctgatt accacctcaa aatcttcata atcctgagca cgaagacatt cgagcgattc
     6961 gcgcaacagc gaagcgccat tatgtacggg cacccctatt cctacacgtg gcaccttgtc
     7021 cgaatcccca tcttcttctt attaatccag cgtcatcctg acggggtcgc ccccacgcac
     7081 atcgggcgcg ttagtgttcc aaagccatca aaatgaaata cctagcggca gcagtactgc
     7141 ggcctcgtgt tgctggcagc atagaggaaa ttatacaatt ggttcttccg cttcagagtc
     7201 tcaggctcat ttttcattga agactggacg cgacaaaacg gcagcagtct cgtagtagac
     7261 tacgcgccaa ttactttctc agcctgagat atctgttttg gaaaaaattg ccgttggggt
     7321 cgccacgcga aaccgacctc ggatgtttgc tcacatgctg gagtcattcc tcaacctcaa
     7381 atggcccgat gattcacaga ttgaatttat ttttcttgag aataatcaca cgttacagat
     7441 tcagaatact gtcgaagatt tcaaggaaaa attgtttaaa aatacaggca atattcctca
     7501 tattcataca acagctgtcg aatcacggca cggcatccct ttcgtacgta accgtattct
     7561 tgatatagct ctggacaagg gatgtgacta ccttgctttt tgcgacgatg accagaccgt
     7621 gaattccgat taccttgtcg aacttgttag agcggccaag gtccgtagtt tggacctagt
     7681 cggaagcata gctagggtag aaacctcgtc cagcgcatca gacttaacgt ttttccagaa
     7741 gctggtttat aacgaattgc gctctcatca agaaaaacaa gagtcgcacg ccatacgcct
     7801 gcatacggcc cacaaagacc aagattggaa accaggctcc ggggccatgc tatgtcgcct
     7861 tgattttctt cgccaacacc gcatccgatt taacgaatct ttgaggtatt cccattgcga
     7921 agacttggat ttttactatc aagtaaaaac tgctggaggg caaaccgggt gtgcaccccg
     7981 agccattgtt tatgaagtca tgcccgaaag tcgattgtcg ccgaccttcc agttcaaaag
     8041 gcacaaaaat ctaacgcttg cacgtcaaaa cattcgttat gcttggggtg tgaaagaaaa
     8101 aaaagttatc cgtttaagtt gtactattct ggggaaatta atattaggta tatctcgaat
     8161 atgcctagcc cctttcaata gaggcagatc attcacacgc ggggtaagag tgcttggtgg
     8221 agcattggga agactccaaa gtttgctagg gcatgaagat aagcactacg tccagacgga
     8281 tggcaactaa aaagacgaaa atacccgtta ttgatgctag gaatttcgaa aaaatggaaa
     8341 tcttgccctg cgctcagctc cagacacgcc tagacacgga agtgctggag catgtttgct
     8401 cctgtatctg cactagagga gatttgcgcg actttccttg ccaacgcatt ctcataacgg
     8461 tctgtatcag atccagcaat taaactccat cagcaacact gtcatcaatg gacacaaagc
     8521 gacccatcat tgtctcagcg gcagacgacg cgtacatgga atttttggat gacatgctcg
     8581 cctccatcca gaaccgcctg catgaatatg atttgggcat cctggatcta ggtctttcaa
     8641 aaaggtcgtt ggctatgatt cgggcacgga aagaagatgc cagagtggtt gatccggggt
     8701 ggcgttacca gccagcagat atgtccaagc agcccccgta caaaaaagtc tattacgcga
     8761 aaccatatct tcccgacatt ttccccggtc atgtcggcta catgtggata gacgcagatg
     8821 tctggttcca agattcctct gctatcgaac actatatttc agcatccgaa aaaactccag
     8881 gtcgaggctc tatcgccttt gagtgtcacc ccaattacaa ggacattaat tacaaaccac
     8941 ggtttcggat ggtgagaaaa tataggattc ttcccgtggc tatacgttta gtaggcagga
     9001 aaacgttccg tctcgtgtac gacatgtttg gagatgaagc tgctcgcaca cacggccttg
     9061 ccccagacaa caacagcgga ttattcttta ttcacgctga ttctcctgct tggaaagcat
     9121 ggcagcacta catgcgaaag gcaaaccatc tcccgttcat ggaccgcaaa tatttccccg
     9181 accaaacgtg tcttaacgtg gcccttcgtg aacgcaacat ctcattccac gtaatgcaac
     9241 ccatatacaa ctggattgtc ggattgggtc tgccgatgct agatgaaaat acacatgcat
     9301 tgctcgaccc atgtagccca cacaaaaaaa tatacgctat tcatctggcc gggagtgcag
     9361 ggtttcagcc atccaatctc cgaaccacca gtggtaatca cgcaagcgtc cagcttaaaa
     9421 tgtccaagtt ttattcggaa accaccgcaa atcaataacc cgctttccga ttaaaattct
     9481 gagaattaga ccattcaatt cattatgttt ttctggttcc tctcccctga agacaaagcc
     9541 gcttttcgaa ggcctcgcgg ataccggacc cctttctcac tccccacaaa atcgcccgaa
     9601 gcaaccaacc tgacgatgct ggaaatggtc atcgaatgtt tggaagacgg tccgctacac
     9661 attttggata taggctgcgg ctctggagcc ttgctacagc gaattgcgga ttactaccga
     9721 gcacagggat ggaatccaga tgaatatctc ctgggaactg acatggatga agatttcttc
     9781 caagccaaag tcccctttca ggtatcagaa ctgacaaaac ccctcaattt ccagatgcgg
     9841 tctttcgacc ttatcatcac tatggaagtg ctagagcatg ttcgttctcc ttatacattg
     9901 ttggaggaaa tttacgcgac tctgaatccc gatgggaaac ttctgttttc agtccccaac
     9961 atgatgacca tcgtcagtcg gtttcgtttt ttgtttacgg gccgattccg gcactatgac
    10021 ggccccagta gcgatcccgc ggatgcggac tcaggtatgg gacatatcaa tccattcccg
    10081 attcagtatt gggattacgg atttcgttat gcaggattta ctgatattcg ttaccgtact
    10141 gaccgtatca agcgcggcgc cttatacttc tctgcatttt taagtccact tatgtggcta
    10201 ggaaaacact ggctgcatag acaggagcgc aataaaaatt tgcgcgtcta ccaacagaat
    10261 cttcgcccgt ttcgcgaaat caactctctc cgcaatcttg ccggacacgg ccttgttgcc
    10321 acttgcacca agtcaggtaa tcctgtatca gaaaagtaag ggtcccaatt ttttatatgg
    10381 ttgttatacg gggccgcgta ataaaaggta aagggggtta tggcaaagca tcccatagct
    10441 cggaagtggc agcttacttt cagaacactc cagtcagggg taccttaaat attgcacttg
    10501 accagcccgt gcgactacgc catgcgaagg cacttatctc tatgatctgg ccttcataca
    10561 tcggaggaat tccttgtctc attacacgta cctcacacac gccgttgcac atcgttgaag
    10621 tgatatctga tgaacaactg ccttgtacgc gcggtgatcg ggtaacgctt gtaatacccc
    10681 gtggatgtac tgttgatttg ccgcccattc accacacaat atggaaagtc ttttatggct
    10741 atgggcgaac tcactggtac gaatacaaaa tttaccgaag gttgttgggc ccgttttatt
    10801 ttgtcaggcg cctatccata caagatccaa aaaggccaga ttaattatcg gccttcacta
    10861 tgcatcgttc tggtctgccg acatcttgga aaactcttct tctgtcaatt tccatggccc
    10921 tcgcctgcgc agttgctcaa tgcgcttctc ctcggacaat ttcgccaata tgccgctcgt
    10981 gatcgcattg cccattgaag gtgtgaattt ctttctgtat atgtccactc gcctcgcgat
    11041 atctatcgct cgaaccaatc catccccgtt ctttctggct tccagcaaaa cctccagaac
    11101 cgcttcctca agaagatgtt gccccctggc ggctttatcc tttatcaatc ggctcatgtc
    11161 aaatattaga cttaggtttc ccgcgacagg catattgcct acaaaaagtg acgcctttca
    11221 atctaaaacc agcccatggt cctgatagat cttcatatgc tccctcgcta tcaactctgg
    11281 actgaattcc ttcacccttt ccagcccctt ctccgacaaa cgttgcctta attcctctga
    11341 ccgagacaga cgaagtaatt gctccgccag tgcgcctgca tctcccggcg ggaccagagc
    11401 cccgcaccct tcggtgatga tttccgggat tccatccacc cggctcgcca caacaggcac
    11461 acccgcacgc atgacatcca gaagcgtgga ccccagcggc tcgaccagag aaggaaatgc
    11521 gaaaagatca aaagcagcaa tccatgtgat gggatcttca acccaacctg caaagtgcac
    11581 attggtcaaa ccttcggcct gctggcggaa ttcttcctcc aagcgaccac tccccagtaa
    11641 aacaaactgg aaatctggcg catcctgctg cagggttcgt gccgcctgaa gcaatacgga
    11701 atgccctttc tccgggccat ccatcgcagc ggcatgcccg acaacgaact tgcctgcaaa
    11761 cttcttttta atctgccgca ctccgtccgg gtcagggtac tccgggttcc aggcatcagg
    11821 aatccggtca attgaagcat ccgggaaacg gtctcgaagg ctgttttcca ctgcttctgt
    11881 caaggccacg acagtctgtg cattccggta tacggcggaa ctgtaggaat tggagtggac
    11941 gggttttgtc aaccggcgcg tgatcaggta ttgcttgcca aacagacttg cagcataagc
    12001 cacctgcgcc gcatgtgctt catgtgcgtg caacaaagat gcacctcggc aaacaaatat
    12061 tgccgatagc cggttcctca cccggcagac ttccaaattt ggacggctat gaaagcgctg
    12121 cgccaaagga ccttgccgta acacaacgac acgctgccta gacacgcctt cggccgcaag
    12181 tgcctcgacc aagatttcag tctgtcgttc tcccccgcgc aactttgggt tgcgcgccaa
    12241 attgatgtgg cataccgaaa tatcgtcccg gagtttcttt cggttccaca atttggcatg
    12301 gtactgggga gattgcgtcg ccatccggcg aatcaaccag aaaggtccga ttagcatccg
    12361 gtaggctttt gacacatacc aacactcccg gaaacgccag aaaaaattcc aggctgcttt
    12421 ggtgccccat gacaaatctt ccaaaacgat ctcttgcgac accttcaatt ccatcgaatc
    12481 cccgtcgcgc aactggaatt tatctcgcaa cttgcacggg gcaacgattt caatgatatg
    12541 cagtggatgg cccttagctc tcgtaatcaa gcagcgcatc cctttgattt cacaagccca
    12601 gaaaaaagtg cgtttccctc ccggaagctc aacctgctct ggatcaaatc ggactggttt
    12661 ctccagcgcc aaattcagcg atccacgaac tgggggctct ccgaaaaaat ttatgacttc
    12721 ggggccaaag gccctgatct ttgaagcagt gccacgtccg ctgattacgc agcctcgcaa
    12781 agtcactaaa atcgacattt gcttaagctc ctaagattag ctctgcgcac tccaaatgcg
    12841 gtgatctcca actgtcaacc gtgttttccc atagagatct ctccttaggg caattttgaa
    12901 ctcagcaagt gcttgctctt tttgttcggc cgaagattca acccccgtgg aaacagtgtc
    12961 taagaaagca tttcccgcct gaatcacttc atcgggacaa tacaaccaag ccagcctcaa
    13021 ttctttgata aatttctctt tttccccttt gtcttgagac aacacataga atccttgtat
    13081 agattcaata aagcccctgt atctgtcttc cttgcgttta tgtttttccc actgaaattt
    13141 tttgcgctcg ttataccacc aaccaaaaat tccagcaatc agcggaacag cgatgcttaa
    13201 cagcggaatg gtttgttcaa gccatatctc catagttcaa gtccatgcgg tagattaatc
    13261 acaaatttta acttagcgtg actaaataac attcggagtt taattaaatt tagaccccta
    13321 tccttgctaa aatgcgccat ccagccatcg catccccatg gagttcctaa aaccccgcac
    13381 cctcaaggcc cgccagaaaa ccctcgccct gctcctgccc tgcctcacac ccgtccagga
    13441 tgaccttgga aatgtcccgg tttcgatgca gcaggaccct cacgtcaatg gtgccttgat
    13501 cggtctgacc gaacgtgtct gcgcccattt tggagtcacc cggcaggcac tcgtccaccg
    13561 cgtgaccgcc gccgtgttcg aggaaatcta ccgccgcgaa gcgaccgctg ttctgacgcg
    13621 gtgcgatgaa tttctggaag atccccagtc cgagttatcc cgcgcgcgtg ccaacatcgg
    13681 agaattgcca tcggaaactc cagatccaaa ctgggtgagc gacctgaacg gctacatcca
    13741 gaaaaactac gaacgccccg acatcctggt gctctgaatt tctgatccat ggacattgcc
    13801 ctgcatcgtt cagcctccga tatcgaggaa gacctggaat actacttcag cgaacagcgc
    13861 gacaagcaca accagattcg cccatcggac gacatccgaa gtttcaccac cgattctcca
    13921 aatttcacgg aggaccccgg agagaacggg atcgggaacg ttttccatcc tttcgcgcca
    13981 ttctcgctgg gccggatttt catcggtatc agtttcccgc tgttctccaa caattggggt
    14041 gcggcgttcc tgcgccatct actgggattg ataaagccca agggagctgt aattctcccg
    14101 gtctatccgg aaatccaggc acgcgaccaa ggtctgtggt gccgcagttc tcttgaaaat
    14161 atcttccgga gccgttcacg ctacatcggc atcagcaaca tctgggcgga aaacgacggc
    14221 gtcatgtcga tgcgggtcgg ccggcgttgg ccacccatca tcccgagtac ggcccggtgg
    14281 ctgttcgaac agactccacg gcgtgcggta gctttggggt tggaagacaa taccgaagca
    14341 atccagaaat tctggcagtc cgaagtccaa cgcttctggc gcctcggtcg ctatcacgcc
    14401 gtcatcgaac agatcattcg caaccaatat ggcccacgcc gacccgtacg tctggccact
    14461 accggcgaag atgcgggccc actcgcgctt gaatgcctat acagccccta tactcgcatc
    14521 accagagccg atgtctacgg tcccaatcta tcccccgatc tcttggaggc cctggatcaa
    14581 gcctgcttga cccaagaaca cggcccactg gaatgccccg gaacctacga cactacaaat
    14641 tcatttgatg tgctgtgtgt cagcggtcct gaacgcctaa gccctgattc tgttggacgc
    14701 atactcacca ccaatgggat attgatcctg acaccagaat gtgccgcttt gaatccggaa
    14761 ctgggtccag gattcaagga accggaatac tattcggaca cggtcgcaca acgccttcac
    14821 cccagcatcc caatctacca ttattccgaa cgaatcgagg aagaaatcgc ccagcaaacc
    14881 gaatcacatc aaagcgcctt catggttctg agcaaggctt agagccactg gtaaattgct
    14941 cctgcgatga acgccacact gacaatcagg gccacggcat tg
//
