BGC0002452 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002452 (Streptomyces kitasatoensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	96,795	High	leucomycin	PKS

BGC0002452 - Region 1 - T1PKS

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Location: 1 - 96,795 nt. (total: 96,795 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 96,796
Label		BGC0002452

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

BBM96622.1: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

lcmBII: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

lcmCX: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

lcmD: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

lcmF: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

lcmI: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acylase acyltransferase aminotransferase glycosyltransferase methyltransferase sulfotransferase transferase

BBM96670.1: Sulfotransferase domain

extras: Sulfotransfer_3: Sulfotransferase domain

lcmA4: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

lcmBI: methyltransferase

smcogs: SMCOG1248: methyltransferase

lcmBIII: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

lcmCII: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

lcmCIII: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

lcmCVIII: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

lcmK: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

lcmL: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

Hydrolases: back translocase esterase phosphatase

BBM96666.1: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

BBM96668.1: inositol monophosphatase

smcogs: SMCOG1207: inositol monophosphatase

BBM96669.1: thioesterase

smcogs: SMCOG1004: thioesterase

lcmG: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

Lyases: dehydratase

lcmCIV: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

lcmCXI: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

lcmJ: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

lcmA1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

lcmA2: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

lcmA3: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

lcmA5: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002452.2	0.97	PKS	leucomycin	Streptomyces kitasatoensis
BGC0000113.5	0.78	PKS	niddamycin	Streptomyces caelestis
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002452.2	1.00	PKS	leucomycin	Streptomyces kitasatoensis
BGC0000113.5	0.78	PKS	niddamycin	Streptomyces caelestis
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 78454:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ohmal - ccmal) + (ccmal) + (Me-ohmal - redemal) + (ohmxmal) + (ohmal)
Non-colinear ordering:: lcmA1 + lcmA2 + lcmA3 + lcmA4 + lcmA5

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

lcmA5: mal

PKS_AT (559..859): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 194.8
inactive: 100.8
Methoxymalonyl-CoA: 66.6
Methylmalonyl-CoA: 50.8
Propionyl-CoA: 28.8
Isobutyryl-CoA: 25.8
Acetyl-CoA: 21.0
Ethylmalonyl-CoA: 19.3
Benzoyl-CoA: 18.0
2-Methylbutyryl-CoA: 11.3
3-Methylbutyryl-CoA: 8.2
trans-1,2-CPDA: 6.2
fatty_acid: 5.0
CHC-CoA: 4.8

lcmA4: mxmal

PKS_AT (569..859): mxmal

ATSignature: Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 91.7%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 197.1
Ethylmalonyl-CoA: 195.9
Methylmalonyl-CoA: 190.3
trans-1,2-CPDA: 122.7
Malonyl-CoA: 110.3
2-Methylbutyryl-CoA: 109.1
Propionyl-CoA: 92.9
CHC-CoA: 86.1
Isobutyryl-CoA: 77.0
Benzoyl-CoA: 56.1
3-Methylbutyryl-CoA: 48.6
Acetyl-CoA: 48.0
fatty_acid: 39.0
inactive: 30.4

lcmA3: mmal - emal

PKS_AT (573..884): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 218.9
Ethylmalonyl-CoA: 212.1
Methoxymalonyl-CoA: 179.4
2-Methylbutyryl-CoA: 154.4
trans-1,2-CPDA: 129.9
Propionyl-CoA: 121.7
CHC-CoA: 105.9
Malonyl-CoA: 105.6
Isobutyryl-CoA: 79.5
Benzoyl-CoA: 78.0
Acetyl-CoA: 38.7
fatty_acid: 38.4
3-Methylbutyryl-CoA: 37.8
inactive: 35.4

PKS_AT (2203..2501): emal

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 233.3
Methylmalonyl-CoA: 203.6
Methoxymalonyl-CoA: 185.3
trans-1,2-CPDA: 156.8
2-Methylbutyryl-CoA: 151.0
Propionyl-CoA: 125.8
CHC-CoA: 101.9
Malonyl-CoA: 92.9
Isobutyryl-CoA: 74.7
Benzoyl-CoA: 62.9
Acetyl-CoA: 57.0
fatty_acid: 51.4
3-Methylbutyryl-CoA: 41.2
inactive: 24.7

lcmA2: mal

PKS_AT (568..862): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.5
inactive: 106.4
Methoxymalonyl-CoA: 63.8
Methylmalonyl-CoA: 62.3
Propionyl-CoA: 37.2
Ethylmalonyl-CoA: 23.2
Acetyl-CoA: 22.3
fatty_acid: 21.1
Isobutyryl-CoA: 20.2
Benzoyl-CoA: 18.8
CHC-CoA: 18.5
trans-1,2-CPDA: 13.1
2-Methylbutyryl-CoA: 13.1
3-Methylbutyryl-CoA: 0.0

lcmA1: mal - mal - mal

PKS_AT (541..833): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.7
inactive: 115.7
Methylmalonyl-CoA: 60.4
Methoxymalonyl-CoA: 59.7
Propionyl-CoA: 35.4
Isobutyryl-CoA: 22.3
Acetyl-CoA: 22.2
CHC-CoA: 21.2
fatty_acid: 17.8
Benzoyl-CoA: 17.7
Ethylmalonyl-CoA: 15.0
trans-1,2-CPDA: 12.4
2-Methylbutyryl-CoA: 11.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (1555..1848): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.5
inactive: 117.8
Methylmalonyl-CoA: 61.9
Methoxymalonyl-CoA: 60.2
Propionyl-CoA: 35.5
Isobutyryl-CoA: 26.2
CHC-CoA: 20.3
fatty_acid: 19.1
Ethylmalonyl-CoA: 19.1
Acetyl-CoA: 18.4
Benzoyl-CoA: 14.2
trans-1,2-CPDA: 12.3
2-Methylbutyryl-CoA: 9.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (3044..3338): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.8
inactive: 112.5
Methylmalonyl-CoA: 64.3
Methoxymalonyl-CoA: 58.2
Propionyl-CoA: 38.4
Isobutyryl-CoA: 29.5
Acetyl-CoA: 22.3
CHC-CoA: 20.2
fatty_acid: 15.8
Benzoyl-CoA: 15.8
Ethylmalonyl-CoA: 15.2
trans-1,2-CPDA: 12.9
2-Methylbutyryl-CoA: 10.6
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor