BGC0002454 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002454 (Streptomyces sp. NRRL S-4)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	97,575	High	venturicidin D/venturicidin E/venturicidin F/venturicidin A	PKS

BGC0002454 - Region 1 - NRPS,T1PKS

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Location: 1 - 97,575 nt. (total: 97,575 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 97,576
Label		BGC0002454

Tailoring functions

Oxidoreductases: oxidoreductase

venM: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: acylase glycosyltransferase transferase

venA: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

venH: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

venJ: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

venO: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

Hydrolases: esterase

venP: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

venB: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

venN: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

QRI43520.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

venF: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

venG: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

venI: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

venK: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002454.2	1.37	PKS	venturicidin D, venturicidin E, venturicidin F, venturicidin A	Streptomyces sp. NRRL S-4
BGC0001273.3	0.92	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.90	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.90	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.78	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002454.2	1.00	PKS	venturicidin D, venturicidin E, venturicidin F, venturicidin A	Streptomyces sp. NRRL S-4
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001268.4	0.66	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 97575:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-mal - Me-ohmal) + (Me-redmal - Me-ohmal) + (Me-redmal - ccmal - ohmal) + (Me-redmal) + (Me-ccmal - Me-ccmal) + (ccmal - Me-mal) + (X - mal - Pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

QRI43520.1: X - mal - Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (37..465): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GALWLGVTAK
Nearest Stachelhaus code(s):: Val DALWLGGTFK (35% 8Å match); bOH-Val DALWLGGTFK (32% 8Å match)
Stachelhaus code match:: 70% (weak)

PKS_AT (1191..1473): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 54.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 107.7
inactive: 49.0
Methoxymalonyl-CoA: 41.9
Methylmalonyl-CoA: 39.0
Propionyl-CoA: 34.9
2-Methylbutyryl-CoA: 16.4
fatty_acid: 16.0
trans-1,2-CPDA: 14.7
Ethylmalonyl-CoA: 14.1
CHC-CoA: 12.7
Benzoyl-CoA: 10.1
Acetyl-CoA: 10.0
Isobutyryl-CoA: 4.0
3-Methylbutyryl-CoA: 0.0

AMP-binding (2596..2987): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFFAQVIK
Nearest Stachelhaus code(s):: Pro DVQFAAQVVK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

venF: mal - mmal

PKS_AT (562..866): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 195.9
inactive: 91.7
Methylmalonyl-CoA: 81.2
Methoxymalonyl-CoA: 73.3
Isobutyryl-CoA: 53.5
Ethylmalonyl-CoA: 44.2
Propionyl-CoA: 41.3
Acetyl-CoA: 24.6
2-Methylbutyryl-CoA: 23.9
Benzoyl-CoA: 17.8
trans-1,2-CPDA: 15.9
fatty_acid: 9.5
3-Methylbutyryl-CoA: 9.0
CHC-CoA: 8.6

PKS_AT (2329..2559): mmal

ATSignature: (unknown)

No matches above 50%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 34.7
Methoxymalonyl-CoA: 32.8
trans-1,2-CPDA: 27.4
Ethylmalonyl-CoA: 21.5
CHC-CoA: 20.0
2-Methylbutyryl-CoA: 14.3
Isobutyryl-CoA: 13.3
Propionyl-CoA: 12.3
Malonyl-CoA: 10.5
3-Methylbutyryl-CoA: 10.5
Benzoyl-CoA: 9.9
inactive: 0.0
fatty_acid: 0.0
Acetyl-CoA: 0.0

venG: mmal - mmal

PKS_AT (566..861): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 190.7
Ethylmalonyl-CoA: 170.6
Methoxymalonyl-CoA: 158.3
2-Methylbutyryl-CoA: 127.9
Propionyl-CoA: 115.9
trans-1,2-CPDA: 111.6
CHC-CoA: 100.8
Malonyl-CoA: 92.9
Isobutyryl-CoA: 67.2
Acetyl-CoA: 66.8
Benzoyl-CoA: 57.9
inactive: 37.0
3-Methylbutyryl-CoA: 35.9
fatty_acid: 21.1

PKS_AT (2316..2621): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.3
Ethylmalonyl-CoA: 187.4
Methoxymalonyl-CoA: 152.6
2-Methylbutyryl-CoA: 115.2
Propionyl-CoA: 114.3
trans-1,2-CPDA: 107.1
Malonyl-CoA: 101.0
CHC-CoA: 92.9
Isobutyryl-CoA: 80.0
Benzoyl-CoA: 59.2
Acetyl-CoA: 46.8
inactive: 40.3
3-Methylbutyryl-CoA: 18.9
fatty_acid: 15.0

venH: mmal

PKS_AT (575..872): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 194.7
Ethylmalonyl-CoA: 178.3
Methoxymalonyl-CoA: 163.5
2-Methylbutyryl-CoA: 113.7
trans-1,2-CPDA: 107.1
Propionyl-CoA: 95.7
Malonyl-CoA: 95.6
CHC-CoA: 91.3
Isobutyryl-CoA: 73.3
Benzoyl-CoA: 54.1
Acetyl-CoA: 50.6
3-Methylbutyryl-CoA: 45.6
inactive: 24.8
fatty_acid: 13.7

venI: mmal - mal - mal

PKS_AT (571..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.6
Ethylmalonyl-CoA: 194.5
Methoxymalonyl-CoA: 169.6
2-Methylbutyryl-CoA: 127.5
trans-1,2-CPDA: 124.9
CHC-CoA: 100.3
Malonyl-CoA: 98.8
Propionyl-CoA: 97.0
Isobutyryl-CoA: 85.9
Benzoyl-CoA: 64.4
3-Methylbutyryl-CoA: 60.0
Acetyl-CoA: 58.0
inactive: 31.2
fatty_acid: 30.9

PKS_AT (2682..2965): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.7
inactive: 98.6
Methylmalonyl-CoA: 40.7
Methoxymalonyl-CoA: 40.1
Propionyl-CoA: 35.6
Benzoyl-CoA: 24.0
Isobutyryl-CoA: 20.9
Acetyl-CoA: 17.2
2-Methylbutyryl-CoA: 16.5
fatty_acid: 16.2
Ethylmalonyl-CoA: 14.5
trans-1,2-CPDA: 9.2
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4460..4743): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.7
inactive: 98.6
Methylmalonyl-CoA: 40.7
Methoxymalonyl-CoA: 40.1
Propionyl-CoA: 35.6
Benzoyl-CoA: 24.0
Isobutyryl-CoA: 20.9
Acetyl-CoA: 17.2
2-Methylbutyryl-CoA: 16.5
fatty_acid: 16.2
Ethylmalonyl-CoA: 14.5
trans-1,2-CPDA: 9.2
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

venJ: mmal - mmal

PKS_AT (569..866): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.6
Ethylmalonyl-CoA: 194.5
Methoxymalonyl-CoA: 169.6
2-Methylbutyryl-CoA: 127.5
trans-1,2-CPDA: 124.9
CHC-CoA: 100.3
Malonyl-CoA: 98.8
Propionyl-CoA: 97.0
Isobutyryl-CoA: 85.9
Benzoyl-CoA: 64.4
3-Methylbutyryl-CoA: 60.0
Acetyl-CoA: 58.0
inactive: 31.2
fatty_acid: 30.9

PKS_AT (2663..2960): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.2
Ethylmalonyl-CoA: 193.5
Methoxymalonyl-CoA: 176.0
2-Methylbutyryl-CoA: 129.6
trans-1,2-CPDA: 121.4
Malonyl-CoA: 107.4
Propionyl-CoA: 90.6
CHC-CoA: 88.9
Isobutyryl-CoA: 88.2
Acetyl-CoA: 63.5
Benzoyl-CoA: 63.0
3-Methylbutyryl-CoA: 59.6
inactive: 36.6
fatty_acid: 26.6

venK: mmal - mmal - mmal

PKS_AT (538..829): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.0
Ethylmalonyl-CoA: 175.2
Methoxymalonyl-CoA: 159.3
2-Methylbutyryl-CoA: 117.2
trans-1,2-CPDA: 109.0
CHC-CoA: 98.1
Propionyl-CoA: 90.7
Malonyl-CoA: 82.1
Isobutyryl-CoA: 62.8
Benzoyl-CoA: 58.7
Acetyl-CoA: 53.5
3-Methylbutyryl-CoA: 45.9
fatty_acid: 35.5
inactive: 26.2

PKS_AT (1555..1852): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.6
Ethylmalonyl-CoA: 194.5
Methoxymalonyl-CoA: 169.6
2-Methylbutyryl-CoA: 127.5
trans-1,2-CPDA: 124.9
CHC-CoA: 100.3
Malonyl-CoA: 98.8
Propionyl-CoA: 97.0
Isobutyryl-CoA: 85.9
Benzoyl-CoA: 64.4
3-Methylbutyryl-CoA: 60.0
Acetyl-CoA: 58.0
inactive: 31.2
fatty_acid: 30.9

PKS_AT (2584..2881): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.6
Ethylmalonyl-CoA: 194.5
Methoxymalonyl-CoA: 169.6
2-Methylbutyryl-CoA: 127.5
trans-1,2-CPDA: 124.9
CHC-CoA: 100.3
Malonyl-CoA: 98.8
Propionyl-CoA: 97.0
Isobutyryl-CoA: 85.9
Benzoyl-CoA: 64.4
3-Methylbutyryl-CoA: 60.0
Acetyl-CoA: 58.0
inactive: 31.2
fatty_acid: 30.9

Link to PARAS substrate predictor