BGC0002456 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002456 (Streptomyces sp. NBRC 109436)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	120,445	High	alchivemycin A/alchivemycin B	PKS+NRPS:Type I

BGC0002456 - Region 1 - NRPS,T1PKS

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Location: 1 - 120,445 nt. (total: 120,445 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 120,446
Label		BGC0002456

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

QSV12647.1: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

QSV12648.1: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

QSV12649.1: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

QSV12651.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

QSV12652.1: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Ligases: ligase synthase

QSV12655.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

QSV12656.1: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

QSV12659.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

QSV12661.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

QSV12662.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

QSV12663.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

QSV12664.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002456.3	1.33	NRPS, PKS	alchivemycin A, alchivemycin B	Streptomyces sp. NBRC 109436
BGC0001273.3	0.93	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.92	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.92	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.80	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.67	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002192.3	0.65	PKS	FR901512	fungal sp. No.14919

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002456.3	1.00	NRPS, PKS	alchivemycin A, alchivemycin B	Streptomyces sp. NBRC 109436
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.71	PKS	oronofacic acid	Pseudomonas syringae
BGC0000018.5	0.70	PKS	angolamycin	Streptomyces eurythermus
BGC0000067.5	0.69	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.67	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 120445:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly) + (mal - Me-ccmal) + (mal - ohmal) + (Me-ohmal - redmal) + (redmal - Me-ccmal - ccmal - ccmal) + (Me-redmal - Me-redmal) + (ccmal - ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 41760:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly) + (mal - Me-ccmal) + (mal - ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 4312 - 120445:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly) + (mal - Me-ccmal) + (mal - ohmal) + (Me-ohmal - redmal) + (redmal - Me-ccmal - ccmal - ccmal) + (Me-redmal - Me-redmal) + (ccmal - ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

QSV12656.1: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (467..935): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code(s):: Gly DILQLGVIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

QSV12655.1: mal - mmal

PKS_AT (623..902): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.7
inactive: 105.7
Methoxymalonyl-CoA: 64.6
Methylmalonyl-CoA: 60.5
Propionyl-CoA: 40.5
Isobutyryl-CoA: 28.2
fatty_acid: 21.9
CHC-CoA: 18.1
Acetyl-CoA: 17.1
Ethylmalonyl-CoA: 14.1
Benzoyl-CoA: 12.7
2-Methylbutyryl-CoA: 10.6
trans-1,2-CPDA: 9.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1661..1955): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.2
Ethylmalonyl-CoA: 200.6
Methoxymalonyl-CoA: 177.8
2-Methylbutyryl-CoA: 133.4
trans-1,2-CPDA: 114.5
Propionyl-CoA: 107.1
Malonyl-CoA: 89.8
CHC-CoA: 88.2
Isobutyryl-CoA: 78.4
Benzoyl-CoA: 75.8
Acetyl-CoA: 66.2
3-Methylbutyryl-CoA: 40.5
inactive: 26.8
fatty_acid: 26.2

QSV12659.1: mal - mal

PKS_AT (612..905): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.6
inactive: 95.6
Methoxymalonyl-CoA: 69.3
Methylmalonyl-CoA: 63.8
Propionyl-CoA: 44.8
Ethylmalonyl-CoA: 35.5
Isobutyryl-CoA: 31.8
2-Methylbutyryl-CoA: 20.3
fatty_acid: 19.2
Acetyl-CoA: 17.6
Benzoyl-CoA: 14.9
trans-1,2-CPDA: 12.0
CHC-CoA: 8.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (1700..1988): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 224.4
inactive: 99.9
Methoxymalonyl-CoA: 66.1
Methylmalonyl-CoA: 61.8
Propionyl-CoA: 45.5
Ethylmalonyl-CoA: 28.2
Acetyl-CoA: 24.0
fatty_acid: 21.0
Isobutyryl-CoA: 20.3
CHC-CoA: 20.1
2-Methylbutyryl-CoA: 17.2
Benzoyl-CoA: 12.8
trans-1,2-CPDA: 11.7
3-Methylbutyryl-CoA: 0.0

QSV12661.1: mmal - mal

PKS_AT (587..881): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.3
Ethylmalonyl-CoA: 203.7
Methoxymalonyl-CoA: 175.9
2-Methylbutyryl-CoA: 135.2
trans-1,2-CPDA: 113.3
Propionyl-CoA: 109.5
Malonyl-CoA: 85.8
CHC-CoA: 84.8
Isobutyryl-CoA: 81.7
Benzoyl-CoA: 77.8
Acetyl-CoA: 61.9
3-Methylbutyryl-CoA: 43.2
fatty_acid: 31.3
inactive: 27.4

PKS_AT (2165..2450): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 225.8
inactive: 106.6
Methoxymalonyl-CoA: 61.4
Methylmalonyl-CoA: 55.6
Propionyl-CoA: 40.4
Isobutyryl-CoA: 20.0
CHC-CoA: 17.7
fatty_acid: 15.7
Ethylmalonyl-CoA: 13.0
Acetyl-CoA: 12.3
Benzoyl-CoA: 12.2
2-Methylbutyryl-CoA: 12.2
trans-1,2-CPDA: 8.0
3-Methylbutyryl-CoA: 0.0

QSV12662.1: mal - mmal - mal - mal

PKS_AT (569..848): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.4
inactive: 101.6
Methoxymalonyl-CoA: 63.0
Methylmalonyl-CoA: 60.2
Propionyl-CoA: 39.4
Isobutyryl-CoA: 27.4
fatty_acid: 18.8
CHC-CoA: 17.0
Acetyl-CoA: 15.5
Ethylmalonyl-CoA: 12.6
Benzoyl-CoA: 12.5
trans-1,2-CPDA: 9.0
2-Methylbutyryl-CoA: 8.9
3-Methylbutyryl-CoA: 0.0

PKS_AT (2707..3001): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.0
Ethylmalonyl-CoA: 202.2
Methoxymalonyl-CoA: 172.6
2-Methylbutyryl-CoA: 128.3
trans-1,2-CPDA: 113.6
Propionyl-CoA: 107.6
CHC-CoA: 92.3
Malonyl-CoA: 83.8
Isobutyryl-CoA: 75.9
Benzoyl-CoA: 74.3
Acetyl-CoA: 56.4
3-Methylbutyryl-CoA: 34.7
fatty_acid: 34.5
inactive: 22.5

PKS_AT (4508..4797): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 223.2
inactive: 106.0
Methoxymalonyl-CoA: 63.6
Methylmalonyl-CoA: 59.6
Propionyl-CoA: 37.8
Isobutyryl-CoA: 27.7
fatty_acid: 18.7
CHC-CoA: 18.1
Acetyl-CoA: 16.0
Benzoyl-CoA: 13.1
Ethylmalonyl-CoA: 12.8
2-Methylbutyryl-CoA: 9.7
trans-1,2-CPDA: 9.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (6309..6583): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 223.5
inactive: 103.4
Methoxymalonyl-CoA: 59.9
Methylmalonyl-CoA: 55.0
Propionyl-CoA: 40.9
Isobutyryl-CoA: 23.1
fatty_acid: 20.1
CHC-CoA: 17.8
Acetyl-CoA: 15.3
Ethylmalonyl-CoA: 10.9
2-Methylbutyryl-CoA: 10.0
Benzoyl-CoA: 9.2
trans-1,2-CPDA: 8.0
3-Methylbutyryl-CoA: 0.0

QSV12663.1: mmal - mmal

PKS_AT (606..900): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.5
Ethylmalonyl-CoA: 199.0
Methoxymalonyl-CoA: 173.2
2-Methylbutyryl-CoA: 134.9
trans-1,2-CPDA: 120.5
Propionyl-CoA: 111.7
CHC-CoA: 88.4
Malonyl-CoA: 86.2
Isobutyryl-CoA: 77.6
Benzoyl-CoA: 77.0
Acetyl-CoA: 62.0
3-Methylbutyryl-CoA: 35.6
fatty_acid: 31.1
inactive: 26.5

PKS_AT (2758..3052): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.5
Ethylmalonyl-CoA: 202.8
Methoxymalonyl-CoA: 177.3
2-Methylbutyryl-CoA: 130.6
trans-1,2-CPDA: 112.3
Propionyl-CoA: 104.9
CHC-CoA: 90.1
Malonyl-CoA: 87.9
Isobutyryl-CoA: 73.9
Benzoyl-CoA: 69.7
Acetyl-CoA: 57.6
3-Methylbutyryl-CoA: 40.0
fatty_acid: 33.2
inactive: 25.6

QSV12664.1: mal - mal

PKS_AT (671..952): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 226.4
inactive: 107.2
Methoxymalonyl-CoA: 71.0
Methylmalonyl-CoA: 62.9
Propionyl-CoA: 42.8
Ethylmalonyl-CoA: 28.4
Isobutyryl-CoA: 23.9
2-Methylbutyryl-CoA: 23.3
CHC-CoA: 21.3
Acetyl-CoA: 21.1
fatty_acid: 20.6
Benzoyl-CoA: 19.2
trans-1,2-CPDA: 11.9
3-Methylbutyryl-CoA: 0.0

PKS_AT (2579..2870): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.3
inactive: 102.2
Methoxymalonyl-CoA: 74.0
Methylmalonyl-CoA: 55.1
Propionyl-CoA: 51.9
2-Methylbutyryl-CoA: 32.2
Isobutyryl-CoA: 23.8
Ethylmalonyl-CoA: 21.0
fatty_acid: 17.5
Acetyl-CoA: 14.6
CHC-CoA: 9.3
trans-1,2-CPDA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor