LOCUS       BGC0002474              7390 bp    DNA     linear   BCT 05-OCT-2006
DEFINITION  Agrobacterium tumefaciens ferric iron uptake system gene cluster
            (agbF, agbC, agbE, agbB, agbA, agbD), complete cds.
ACCESSION   BGC0002474
VERSION     BGC0002474
KEYWORDS    .
SOURCE      Agrobacterium tumefaciens (Rhizobium radiobacter)
  ORGANISM  Agrobacterium tumefaciens
            Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
            Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
            Agrobacterium tumefaciens complex.
COMMENT     On Mar 3, 2003 this sequence version replaced AB083344.1.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 09:51:36
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7390
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /chromosome="Linear Chromosome"
                     /clone="Fosmid 1A3"
                     /clone_lib="Genomic Library in Fosmid pBeloBAC11"
                     /country="Japan"
                     /db_xref="taxon:358"
                     /map="SwaI-#4/PmeI-#3 Region"
                     /mol_type="genomic DNA"
                     /organism="Agrobacterium tumefaciens"
                     /strain="MAFF301001"
     subregion       1..7390
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="NRP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7390
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(104..1750)
                     /gene="agbF"
     CDS             complement(104..1750)
                     /NRPS_PKS="Domain: Heterocyclization (0-266). E-value:
                     3.6e-59. Score: 192.4. Matches aSDomain:
                     nrpspksdomains_agbF_Heterocyclization.1"
                     /codon_start=1
                     /function="iron uptake/ Agrobactin biosynthesis"
                     /gene="agbF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     151.8; E-value: 5.6e-46)"
                     /gene_kind="biosynthetic-additional"
                     /product="probable non-ribosomal peptide synthase"
                     /protein_id="BAC57933.1"
                     /sec_met_domain="Condensation (E-value: 2.5e-32, bitscore:
                     100.8, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDAEKLAVAVESLCRRHPMLSLRIDAEGRQSVDPVGRPLRLDVED
                     MTALDSEALARRLETKREEWSHRGADLRAAAPAAISLSLLPEGRCRLHVDTDMLAIDPA
                     SVRIVMEDLARFYETAAGVDETLTFESVPPSFFDWAERLASDPDLRGRREKDRLWWRQR
                     LADVPDAPPLPFLPDDPSRAVHTDRLATMLSAAERGSLEQTARRCSVTPSTLLLGVFAV
                     ALSQAAGAEKFRLSVPGFWRAPLVEGVDEIVGEFSNVLVLGVEIRPEESVEALLGRLFD
                     EMNGLLAHQSYPGVSVMRDLSRLRGGLQTSPVVFTAGVDLPGGELFSERVGRVFGPMVH
                     TVSQGPGVALDAQVAALDGGLLINWDVRLDALPESWLRALFETYVALLRRLAESPDLTS
                     ASVERLVARQPEAAAEAKGKPMERPLSPLQQAYLLGRGTHLPLSGVAMQEFREYRGNID
                     PELLQSRLAAMVGRHESLRTRIDERRLVQIVSDEPKINLDRVDLSALICSGSRGKGRGG
                     ATGFFPCTFRSRSLAMADHDLPYAGYRDGGA"
     CDS_motif       complement(311..391)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_agbF_0007"
                     /evalue="1.00E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="agbF"
                     /protein_end="480"
                     /protein_start="453"
                     /score="21.2"
                     /tool="antismash"
                     /translation="RGNIDPELLQSRLAAMVGRHESLRTRI"
     CDS_motif       complement(653..727)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_agbF_0006"
                     /evalue="2.80E-03"
                     /label="Cy7"
                     /locus_tag="agbF"
                     /protein_end="366"
                     /protein_start="341"
                     /score="10.9"
                     /tool="antismash"
                     /translation="VSQGPGVALDAQVAALDGGLLINWD"
     CDS_motif       complement(848..892)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_agbF_0005"
                     /evalue="4.30E-05"
                     /label="afterCy5"
                     /locus_tag="agbF"
                     /protein_end="301"
                     /protein_start="286"
                     /score="16.2"
                     /tool="antismash"
                     /translation="LAHQSYPGVSVMRDL"
     aSDomain        complement(953..1750)
                     /aSDomain="Heterocyclization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_agbF_Heterocyclization.1"
                     /evalue="3.60E-59"
                     /label="agbF_Heterocyclization.1"
                     /locus_tag="agbF"
                     /protein_end="266"
                     /protein_start="0"
                     /score="192.4"
                     /tool="antismash"
                     /translation="MDAEKLAVAVESLCRRHPMLSLRIDAEGRQSVDPVGRPLRLDVED
                     MTALDSEALARRLETKREEWSHRGADLRAAAPAAISLSLLPEGRCRLHVDTDMLAIDPA
                     SVRIVMEDLARFYETAAGVDETLTFESVPPSFFDWAERLASDPDLRGRREKDRLWWRQR
                     LADVPDAPPLPFLPDDPSRAVHTDRLATMLSAAERGSLEQTARRCSVTPSTLLLGVFAV
                     ALSQAAGAEKFRLSVPGFWRAPLVEGVDEIVGEFSNVLVLGVEI"
     CDS_motif       complement(956..1009)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_agbF_0004"
                     /evalue="1.60E-04"
                     /label="Cy5"
                     /locus_tag="agbF"
                     /protein_end="265"
                     /protein_start="247"
                     /score="14.2"
                     /tool="antismash"
                     /translation="GVDEIVGEFSNVLVLGVE"
     CDS_motif       complement(1076..1123)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_agbF_0003"
                     /evalue="8.40E-04"
                     /label="Cy4"
                     /locus_tag="agbF"
                     /protein_end="225"
                     /protein_start="209"
                     /score="12.4"
                     /tool="antismash"
                     /translation="VTPSTLLLGVFAVALS"
     CDS_motif       complement(1436..1480)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_agbF_0002"
                     /evalue="3.30E-04"
                     /label="Cy3"
                     /locus_tag="agbF"
                     /protein_end="105"
                     /protein_start="90"
                     /score="13.9"
                     /tool="antismash"
                     /translation="RLHVDTDMLAIDPAS"
     CDS_motif       complement(1661..1714)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_agbF_0001"
                     /evalue="4.30E-06"
                     /label="Cy2"
                     /locus_tag="agbF"
                     /protein_end="30"
                     /protein_start="12"
                     /score="19.1"
                     /tool="antismash"
                     /translation="LCRRHPMLSLRIDAEGRQ"
     gene            1949..2977
                     /gene="agbC"
     CDS             1949..2977
                     /codon_start=1
                     /function="iron uptake"
                     /gene="agbC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018:isochorismate synthase (Score: 184.1; E-value:
                     9.8e-56)"
                     /gene_kind="biosynthetic-additional"
                     /product="probable isochorismate synthase"
                     /protein_id="BAC16757.2"
                     /transl_table=11
                     /translation="MKTGALRTNDVESDEITEFNREFLFTSGSRQLRARGIRERIAIPA
                     RGGEDISSPLQQALADAFERARRDGEQNPIAIGAIPFDVSEPSCLYIPYNHEWNSRDAA
                     PIVADSAMPALVGQNSLPDETGFKRAVEHAIVNFRHSEVRKAVLSVMRELTFASEVDVE
                     RLLASLAKQNREGYQFRIPLPDGGDLIGVSPELLIHKQGEKIISNPLAGSAKRMVDPAA
                     DKANADRLSASEKDHYEHSLVIEDIRSLLSPVCAELDVPDKPSLINTAALWHLSTRIEG
                     RLADPETMRCSLLACCTPHRPSAASPPSAPIASSASSSLSSAGSSPAWSAGRILKVMAN
                     GW"
     gene            3142..4767
                     /gene="agbE"
     CDS             3142..4767
                     /NRPS_PKS="Domain: AMP-binding (31-439). E-value: 2.3e-83.
                     Score: 271.9. Matches aSDomain:
                     nrpspksdomains_agbE_AMP-binding.1"
                     /codon_start=1
                     /function="iron uptake/ Agrobactin biosynthesis"
                     /gene="agbE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     444.1; E-value: 8.7e-135)"
                     /gene_kind="biosynthetic-additional"
                     /product="probable 2,3-dihydroxybenzoate-AMP ligase
                     protein"
                     /protein_id="BAC16758.1"
                     /sec_met_domain="AMP-binding (E-value: 3.3e-88, bitscore:
                     284.6, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIEFERWPEDFVRRYRERGYWTDRPLSGILTEQVERRPDATALI
                     CGDRRFSYADLDRQSSNLAGHLSAAGVGKGDTALVQLPNIAEFYLVFFALMKIGAAPVN
                     ALFSHRRLEMTSYAEQIAPKLVIASRSHELFADDAFVETLKAVSPKLAVTLLLGEADPQ
                     RSLERMLANSAENPPAYAPSASDEVALFQLSGGSTGTPKLIPRTHNDYDYSARASAEIC
                     ELSPETRFLCAIPVAHNYPMSSPGALGVFHAGGTVVMAANPEPLACFDLIEKHEIDMVP
                     LVPPAVALWLQAAPAHRERLKSLKLLQVGGASFAEALARQVPEVLGCDLQQVFGMAEGL
                     VNYTRAGDPDHIVFTTQGRPISPDDEIRIVDEDGNDVPEGEAGMLATRGPYTFRGYYRA
                     PEHSARVFDRDGFYYSGDVVQRTPEGYLRVVGRVKDQINRGGEKVASEEVENLILRHPD
                     VTHAALVAMHDELLGEKSCVFVVSRNPALKAPAIRQHLAGLGVADYKLPDRVRFIEAMP
                     LTAVGKIDKKRLRDLLVTPVAISA"
     aSDomain        3235..4458
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_agbE_AMP-binding.1"
                     /evalue="2.30E-83"
                     /label="agbE_AMP-binding.1"
                     /locus_tag="agbE"
                     /protein_end="439"
                     /protein_start="31"
                     /score="271.9"
                     /tool="antismash"
                     /translation="TEQVERRPDATALICGDRRFSYADLDRQSSNLAGHLSAAGVGKGD
                     TALVQLPNIAEFYLVFFALMKIGAAPVNALFSHRRLEMTSYAEQIAPKLVIASRSHELF
                     ADDAFVETLKAVSPKLAVTLLLGEADPQRSLERMLANSAENPPAYAPSASDEVALFQLS
                     GGSTGTPKLIPRTHNDYDYSARASAEICELSPETRFLCAIPVAHNYPMSSPGALGVFHA
                     GGTVVMAANPEPLACFDLIEKHEIDMVPLVPPAVALWLQAAPAHRERLKSLKLLQVGGA
                     SFAEALARQVPEVLGCDLQQVFGMAEGLVNYTRAGDPDHIVFTTQGRPISPDDEIRIVD
                     EDGNDVPEGEAGMLATRGPYTFRGYYRAPEHSARVFDRDGFYYSGDVVQRTPEGYLRVV
                     GRVKDQINR"
     CDS_motif       4273..4362
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_agbE_0001"
                     /evalue="1.20E-03"
                     /label="NRPS-A_a6"
                     /locus_tag="agbE"
                     /protein_end="407"
                     /protein_start="377"
                     /score="11.3"
                     /tool="antismash"
                     /translation="PEGEAGMLATRGPYTFRGYYRAPEHSARVF"
     gene            4860..5729
                     /gene="agbB"
     CDS             4860..5729
                     /NRPS_PKS="Domain: PP-binding (221-278). E-value: 1.2e-12.
                     Score: 40.1. Matches aSDomain:
                     nrpspksdomains_agbB_PP-binding.1"
                     /codon_start=1
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="iron uptake/ Agrobactin biosynthesis"
                     /gene="agbB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:isochorismatase (Score: 358.7; E-value:
                     2.5e-109)"
                     /gene_kind="biosynthetic-additional"
                     /product="probable 2,3-dihydro-2,3-dihydroxybenzoate
                     synthetase, isochorismatase protein"
                     /protein_id="BAC16759.1"
                     /sec_met_domain="PP-binding (E-value: 1.3e-13, bitscore:
                     40.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIPSISDYPMPVAASFPANKTKWQPDAKRAVLLIHDMQRYFLRF
                     YEADGKLMTTLIDNLAKVKAWAVQNSVPVIYTAQPHDQPPGDRALLNDMWGPGLTVVDP
                     ELQKVVDKLAPTPQDVVLTKWRYSAFQRSDLTERMKGWGRDQIIVGGVYAHIGCMMTAV
                     EAFMKDIQPFMIGDGVADFSEAEHRMALQYVATRCGVVIDTASLVGAGEATDISEWLKA
                     RVLQMVEDESDIDPDENLIFYGLDSLQVMKLAGELKERGVTVGFDELARVPTLSAWWAL
                     IEEKRLAA"
     aSDomain        5523..5693
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_agbB_PP-binding.1"
                     /evalue="1.20E-12"
                     /label="agbB_PP-binding.1"
                     /locus_tag="agbB"
                     /protein_end="278"
                     /protein_start="221"
                     /score="40.1"
                     /tool="antismash"
                     /translation="ARVLQMVEDESDIDPDENLIFYGLDSLQVMKLAGELKERGVTVGF
                     DELARVPTLSAW"
     gene            5743..6498
                     /gene="agbA"
     CDS             5743..6498
                     /NRPS_PKS="Domain: PKS_KR (10-160). E-value: 2e-12. Score:
                     39.2. Matches aSDomain: nrpspksdomains_agbA_PKS_KR.1"
                     /codon_start=1
                     /function="iron uptake/ Agrobactin biosynthesis"
                     /gene="agbA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     227.6; E-value: 2.7e-69)"
                     /gene_kind="biosynthetic-additional"
                     /product="probable 2,3-dihydro-2,3-dihydroxybenzoate
                     dehydrogenase protein"
                     /protein_id="BAC16760.1"
                     /sec_met_domain="adh_short (E-value: 2.9e-35, bitscore:
                     110.5, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQQRFTGRRVLVTGTAQGIGRRIAERFIEEGAEVIGLDLLARTT
                     EAPFRQIALDITDATAVEKACKAIESEWGGLDILVNAAGILRLGDSESLTPADWKACMD
                     VNAAGPFYMLRQWSSIFKRQRRGAIVNVGSNAAVVPRIGMMAYCASKAALVSLSHCAAL
                     ELAPYGVRCNVVSPGSTDTPMLAGMLSDPAGKERLVAGLPEQFKLGIPLGKIARPDDIA
                     DTVLFLASDQAGHVTMQQIVVDGGATLAA"
     aSDomain        5773..6222
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_agbA_PKS_KR.1"
                     /evalue="2.00E-12"
                     /label="agbA_PKS_KR.1"
                     /locus_tag="agbA"
                     /protein_end="160"
                     /protein_start="10"
                     /score="39.2"
                     /tool="antismash"
                     /translation="VLVTGTAQGIGRRIAERFIEEGAEVIGLDLLARTTEAPFRQIALD
                     ITDATAVEKACKAIESEWGGLDILVNAAGILRLGDSESLTPADWKACMDVNAAGPFYML
                     RQWSSIFKRQRRGAIVNVGSNAAVVPRIGMMAYCASKAALVSLSHC"
     CDS_motif       5776..5844
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_agbA_0001"
                     /evalue="1.20E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="agbA"
                     /protein_end="34"
                     /protein_start="11"
                     /score="14.7"
                     /tool="antismash"
                     /translation="LVTGTAQGIGRRIAERFIEEGAE"
     gene            6529..7254
                     /gene="agbD"
     CDS             6529..7254
                     /NRPS_PKS="Domain: ACPS (123-182). E-value: 1.1e-09. Score:
                     30.1. Matches aSDomain: nrpspksdomains_agbD_ACPS.1"
                     /codon_start=1
                     /function="iron uptake/ Agrobactin biosynthesis"
                     /gene="agbD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1060:4'-phosphopantetheinyl transferase (Score: 156.5;
                     E-value: 1.2e-47)"
                     /gene_kind="biosynthetic-additional"
                     /product="probable phosphopantetheinyl transferase"
                     /protein_id="BAC57934.1"
                     /transl_table=11
                     /translation="MAVATAFHREAVSGDPPEAIWPWPDDSGDCHCLTLRYSAEEGGGG
                     DVPPLPPQLLNAAQRRKAEFIAGRRCAAEATRRLTGYAVFPGMGEDRAPIWPEGVIGAI
                     SHSHGRAIALAGSSQRFRGIGIDIERLLTEEEARDIAPQALTANERHNLGNDIDPFMTG
                     LIFSAKESLFKALYPTVKRLFFFEAAELSGFDANGSASLRLTADLGEDWRRGNGGSLPL
                     LPLRRPAAHSHPSPALREK"
     aSDomain        6898..7074
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_agbD_ACPS.1"
                     /evalue="1.10E-09"
                     /label="agbD_ACPS.1"
                     /locus_tag="agbD"
                     /protein_end="182"
                     /protein_start="123"
                     /score="30.1"
                     /tool="antismash"
                     /translation="IGIDIERLLTEEEARDIAPQALTANERHNLGNDIDPFMTGLIFSA
                     KESLFKALYPTVKR"
ORIGIN
        1 ggcggtttct ggcggcaaga cgccatcgaa gagttccgct gcaaggctgg caatggcccg
       61 cccatcaagg ataagcgcat cgaaacgcag gaaaacgaca actttatgcc ccgccgtccc
      121 ggtatccggc atatggaaga tcgtgatctg ccatggcgag gctgcgagat cgaaatgtgc
      181 atgggaaaaa tcccgtcgcg ccgcctcgac ccttgcctcg gcttccggaa cagatcaggg
      241 ccgaaagatc gacacgatcg agattgatct tcggctcgtc ggagacgatc tgcaccagac
      301 gccgctcatc gatgcgggta cgcaggcttt catgtctccc gaccattgcg gcaaggcgcg
      361 actgcaacag ttccgggtcg atattgccgc gatattcgcg gaactcctgc atcgccacac
      421 cgctgagcgg cagatgggtg ccgcgcccga gcagataggc ctgttgcagg ggtgagagtg
      481 gacgttccat gggttttcct tttgcctcgg ctgccgcttc cggctgccgt gcgaccaatc
      541 gctccacgct tgcagaagta agatcggggc tttccgcgag ccggcggaga agagcgacgt
      601 aggtttcgaa caaagcgcgc agccagctct ccggcagcgc atcaagccgc acgtcccagt
      661 tgatcagcag gccaccatcc agagcagcca cctgcgcatc cagcgcgacg cccggcccct
      721 gcgagacggt gtggaccatc gggccgaaca cccgcccgac ccgttccgaa aacagctctc
      781 cgcccggaag atcgaccccg gcggtaaaga ccaccggcga ggtctgcaaa ccaccgcgca
      841 ggcgggagag atcgcgcatg acgctgacgc ccggatagga ctggtgcgcg agaaggccgt
      901 tcatttcatc gaacagccgg ccgagcagcg cctcgacgct ttcttcaggc ctgatttcca
      961 cgccgagcac gagcacgttg gaaaattcgc cgacgatctc atcgacccct tccaccaacg
     1021 gcgcgcgcca gaaacccggc acggaaagcc ggaatttctc cgcccccgcc gcctgcgaca
     1081 gagccaccgc gaaaacgccg agcagaaggg tcgatggcgt cacggagcag cgccgcgccg
     1141 tctgctccag actgccgcgc tcagccgctg aaagcattgt ggcgagccgg tccgtgtgaa
     1201 cggcgcgcga ggggtcatcg ggcagaaacg gtagcggtgg agcgtccgga acatcggcaa
     1261 gcctttgccg ccaccataaa cggtcctttt cgcgccggcc cctcaaatca ggatcggacg
     1321 caagacgctc cgcccagtcg aaaaacgacg ggggcactga ttcgaacgtc agggtttcgt
     1381 ctacgcctgc cgccgtctcg taaaatcggg caaggtcttc catcacgatc cgcacgctgg
     1441 ccggatcgat ggccagcata tcggtgtcca catgcagacg gcaacgccct tccggcaaca
     1501 ggctgagcga aatcgccgcc ggtgcggcag cacgaagatc agccccgcga tggctccact
     1561 cctcgcgttt cgtctcaaga cggcgcgcaa gcgcctccga atcaagcgcg gtcatgtctt
     1621 ccacatcgag gcggagcggc cggccgacag gatcgacgga ctgacgccct tccgcgtcga
     1681 tacggaggct cagcatggga tgacgccggc agagactttc gacggcaacg gccagttttt
     1741 ccgcatccac cgcctgcccc tcgaattcga ggtaaagatg gggcgtaact ttcccgagta
     1801 ttccatccgc ctcgcggccg acccaatagg ccacctgcat cggggttaaa tttagcatgt
     1861 gagtttgaac cggttgacaa aacatgatta gcatactcaa gtataagcca tcgtcaacca
     1921 ctcgaaccac tcgcacagga ggcatcccat gaaaacggga gcactcagga cgaatgacgt
     1981 cgaatccgac gaaattactg aatttaatag agaatttctg tttacttccg gcagcaggca
     2041 attacgcgcc aggggcattc gcgaaaggat cgcgattccc gcccgtggcg gcgaagacat
     2101 atcgagcccg ctgcagcagg cactggccga cgccttcgag cgtgcgcgcc gggacggcga
     2161 gcagaacccc atcgccatcg gcgcaatccc attcgatgtc agcgaacctt catgcctgta
     2221 tattccatac aaccatgaat ggaattcaag ggatgccgcg ccgatcgtcg ccgattcggc
     2281 catgccggcg cttgtcggcc agaacagcct gccggacgag accggtttca agcgtgccgt
     2341 cgagcatgcc atcgtcaatt tccgtcattc cgaagtccgc aaggcggtgc tttcggtcat
     2401 gcgcgaactg accttcgcct ccgaggtgga tgtcgaaaga ttgctggcga gcctcgccaa
     2461 gcagaaccgc gaaggttacc agttccgcat tccgcttccc gatggcggcg acctgatcgg
     2521 cgtcagcccg gaactcctga tccacaagca gggcgagaaa atcatctcga acccgctggc
     2581 gggatcggca aaacgcatgg ttgatccggc ggcggacaag gccaatgcgg atcggctctc
     2641 ggcttccgaa aaggaccatt acgagcacag cctggtgatt gaggatatcc gctcgctgct
     2701 ttcaccggtc tgcgccgaac tcgatgttcc cgacaaaccc tccctcatca acaccgcggc
     2761 actctggcat ctttcaacgc gtatcgaagg ccgccttgcc gatcccgaga caatgcgctg
     2821 cagcttgctt gcctgctgca ccccacaccg gccgtctgcg gcttccccac cgagcgcgcc
     2881 catcgcctca tccgcttcgt cgagcctttc gagcgcgggc tcttcaccgg catggtcggc
     2941 tggacggatt ctgaaggtaa tggcgaatgg gtggtgacta tccgttgcgg caccgtgcag
     3001 cgggaaacag tgcggctctt cgccggcgcc ggcatcgtgg aggcctcgga gcccgaatcc
     3061 gaatgggccg aggtgcagac aaaacttaag accatgctga acgcctgcgg tctttctgcc
     3121 taattttccc aggaacgaac catgacaatc gaatttgaac gctggcccga ggatttcgtt
     3181 cgccgatacc gcgaacgcgg ctactggacc gaccggccgc tgagcggcat cctcaccgaa
     3241 caggtggagc gccggccgga tgcgacggcg ctgatctgcg gcgaccgccg cttcagctat
     3301 gccgatctcg accggcaatc ctccaatctt gccggacatc tctccgccgc gggcgttggc
     3361 aaaggtgaca ctgcgctagt gcaattgcct aacatcgccg agttttatct cgtctttttc
     3421 gcgctgatga aaatcggcgc agcccccgtc aacgcacttt tcagccatcg ccggctggaa
     3481 atgacctcct atgcggagca gattgcaccg aagctggtga ttgcctcgcg cagccacgag
     3541 cttttcgccg atgacgcatt tgtggagacg ctgaaagcag tctccccgaa gcttgcggtg
     3601 accctgctgc tcggcgaagc ggatccgcag cgcagccttg agcggatgct tgccaattcc
     3661 gccgaaaacc cgcccgccta cgccccttcg gcgtcagacg aggtggcgtt gttccagctt
     3721 tccggcggca gcaccggaac cccgaaactg atcccgagga cgcacaacga ctatgattac
     3781 agcgcccggg ccagcgcgga gatttgcgaa ctctcgcctg agacccgttt cctttgcgcc
     3841 attccggtgg cgcataatta cccgatgagc tcgcccggcg cgctcggcgt tttccacgcc
     3901 ggcggcaccg tggtcatggc cgccaatccg gagccgcttg cctgcttcga tctcatcgaa
     3961 aaacacgaga tcgacatggt gccgctggtg ccgccggccg tggcgctgtg gctgcaggct
     4021 gcacccgccc atcgtgaacg gctgaaatcc ctcaagctcc tgcaggtcgg cggcgcaagc
     4081 tttgccgaag ccctggcccg ccaggtgcca gaagtgctgg gctgcgacct gcagcaggta
     4141 ttcggcatgg cggaagggct ggtgaactac acccgcgccg gtgatcccga ccatatcgtt
     4201 ttcaccacgc aagggaggcc gatcagcccg gatgacgaga tccgcatcgt cgacgaggac
     4261 ggcaacgatg tgcccgaggg cgaagccggc atgctggcga cccgtggccc ctatacgttc
     4321 cggggctatt accgcgcgcc cgaacatagc gcccgggtct tcgacaggga tgggttctat
     4381 tattccggcg atgtcgtgca gcgcacgccc gaaggttatc tccgggtcgt tggccgggtg
     4441 aaggaccaga tcaatcgcgg cggcgaaaag gtcgcttcgg aggaggtcga aaaccttatc
     4501 ctgcggcatc cggacgtcac ccatgcagca ctcgtggcga tgcacgacga gcttctaggt
     4561 gagaaaagct gcgtcttcgt cgtttcacgc aatcccgcgc tcaaggcgcc ggccatccgc
     4621 cagcatctcg ccggtcttgg cgtcgcggat tacaagctgc cggaccgtgt gcgcttcatc
     4681 gaggccatgc cgctcaccgc cgtcggcaag atcgacaaga aacgtctgcg cgacctgctt
     4741 gtgacacccg tcgccatcag cgcctgacac gcagcacaaa acaaagggga cgctcaaaaa
     4801 agagcgtccc gaaaccagag attaagcgcc ccctggcgca gcaaaccacc gaggtcatca
     4861 tgacgatccc cagcatttcc gactatccga tgccggttgc cgcaagcttt ccggccaaca
     4921 agacaaaatg gcagcctgac gcgaaacgcg ccgtgctcct catccacgac atgcagcgtt
     4981 atttcctgcg gttctacgag gccgacggca agctgatgac gaccctcatc gacaatctcg
     5041 caaaggtgaa ggcttgggcg gttcagaaca gcgtacctgt tatctacacc gcccagccgc
     5101 atgatcagcc gccgggcgac cgtgcgctcc tgaacgacat gtggggaccg ggtctcaccg
     5161 tcgtcgatcc cgaactgcag aaggtggtgg ataagctcgc cccgacacct caggacgtgg
     5221 tgctgaccaa atggcgttac agcgccttcc agcgctccga ccttactgag cgcatgaaag
     5281 gctggggccg tgaccagatc atcgtcggtg gcgtttacgc ccatatcggc tgcatgatga
     5341 cggcggtgga agccttcatg aaggatattc agcccttcat gatcggtgac ggcgttgccg
     5401 atttctcgga agccgaacac cgcatggcgc tgcaatatgt ggcgacacgc tgcggcgtgg
     5461 tgatcgacac cgcaagcctt gtcggcgcgg gagaagccac cgatatcagc gaatggctga
     5521 aagcccgcgt cctgcaaatg gtggaggatg aaagcgatat cgaccccgac gagaacctga
     5581 tcttctacgg gctggattcg ctgcaggtga tgaagcttgc cggcgagctg aaggaacgcg
     5641 gtgtcaccgt cggttttgac gagcttgcgc gtgtacccac actttccgcc tggtgggcgc
     5701 tgatcgaaga aaagcggctc gcagcctgag cggacgaaaa agatgacgca acagagattt
     5761 accgggcggc gtgtgctggt gacgggcacg gcccagggca tcggccgccg catcgccgaa
     5821 cgtttcatcg aggaaggggc cgaggtcatc ggcctcgacc ttctggcaag aacgacggaa
     5881 gcgcccttcc gccagatcgc actcgacatc accgacgcga cggcggtgga gaaggcctgc
     5941 aaggcaatcg aaagcgaatg gggcgggctc gatatcctgg tcaacgccgc cggcatcttg
     6001 cggctcggcg acagcgaaag cctgacgcct gcggactgga aagcctgcat ggacgtcaat
     6061 gcagcgggac cgttttatat gctgcggcaa tggagcagca tcttcaaacg gcagcggcgc
     6121 ggtgcgatcg tcaatgtcgg ctccaatgcc gccgtcgtgc cgcgcatcgg catgatggcc
     6181 tattgcgcct ccaaggcggc gctggtcagc ctcagccatt gcgccgccct tgagcttgca
     6241 ccctatggcg tgcgctgcaa cgtcgtttca ccgggctcca ccgatacgcc gatgcttgcc
     6301 ggcatgttga gcgatcccgc cggcaaggaa cggctggtcg ccggcctgcc cgagcagttc
     6361 aagctcggca ttccgctcgg caagatcgca aggcctgatg atatcgccga tacggtgctg
     6421 tttctcgcct ccgatcaggc cgggcatgtg acgatgcagc agatcgtggt tgatggcggc
     6481 gccacgcttg cggcttaaat tcgcgccgtt ggattgaagg tgaagaatat ggccgtcgca
     6541 acggcgtttc atcgcgaagc cgtctccggc gatccaccgg aggccatctg gccttggccg
     6601 gacgacagcg gcgattgcca ttgcctcacc cttcgttaca gcgccgaaga aggcggcggc
     6661 ggcgatgtcc ctcccttgcc gccgcagctt ttaaacgccg cgcagagacg caaggcggaa
     6721 ttcatcgccg gccgcagatg cgcggcggaa gccacgcgcc gcctcaccgg ctacgccgtc
     6781 tttcccggta tgggtgagga tcgcgccccg atctggcccg agggcgtcat cggtgcgatt
     6841 tcccacagcc atgggcgggc gattgcgctc gccggctctt cccagcgatt tcgcggcatc
     6901 ggcatcgata tcgaaaggct gctgaccgaa gaggaggccc gcgatatcgc cccgcaggcc
     6961 ttgaccgcga atgagcgcca caacctcggc aacgatatcg atcccttcat gaccgggttg
     7021 attttttcgg ccaaggaaag cctgttcaag gcgctttatc cgacagtgaa acggctgttc
     7081 tttttcgagg cggccgaatt atccggcttt gacgcaaacg gctccgcatc attgcgcctg
     7141 acggccgacc tcggcgaaga ctggcgccgc ggaaacggag gttcccttcc gcttctgccg
     7201 cttcgaaggc ctgctgctca ctcgcatcct tctcccgcat tgagggaaaa atagtagatt
     7261 cataactgag aagatcatat tttaaacccg tcttcccgtc atccgagtga aacactcgga
     7321 aggtacgcag cctgttggaa tgattccaaa cattgaacgg tggagacaga ctatgaccga
     7381 catcgatacg
//
