LOCUS       BGC0002476             10330 bp    DNA     linear   BCT 17-NOV-2022
DEFINITION  Escherichia coli str. K-12 substr. MG1655, complete genome.
ACCESSION   BGC0002476
VERSION     BGC0002476
KEYWORDS    .
SOURCE      Escherichia coli str. K-12 substr. MG1655
  ORGANISM  Escherichia coli str. K-12 substr. MG1655
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     On Sep 26, 2013 this sequence version replaced U00096.2.
            On September 24, 2018 changes were made to proteins and annotation
            On September 23, 2020 changes were made to proteins and annotation
            On March 7, 2022 changes were made to the proteins and annotation.
            Current U00096 annotation updates are derived from EcoCyc
            https://ecocyc.org/.  Suggestions for updates can be sent to
            biocyc-support@ai.sri.com. These updates are being generated from a
            collaboration  that includes EcoCyc, the University of Wisconsin,
            UniProtKB/Swiss-Prot, and the National Center for Biotechnology
            Information (NCBI).
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:47:56
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..816)
                     /EC_number="7.2.2.17"
                     /codon_start=1
                     /db_xref="UniProtKB/Swiss-Prot:P23878"
                     /db_xref="ASAP:ABE-0002025"
                     /db_xref="ECOCYC:EG10295"
                     /gene="fepC"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 224.3; E-value:
                     2.7e-68)"
                     /gene_kind="transport"
                     /gene_synonym="ECK0581"
                     /locus_tag="b0588"
                     /product="ferric enterobactin ABC transporter ATP binding
                     subunit"
                     /protein_id="AAC73689.1"
                     /transl_table=11
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGCG
                     KSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELVAR
                     GRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQETA
                     IMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALREGKI
                     VAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     subregion       1..10330
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002476"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10330
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..816)
                     /db_xref="ASAP:ABE-0002025"
                     /db_xref="ECOCYC:EG10295"
                     /gene="fepC"
                     /gene_synonym="ECK0581"
                     /locus_tag="b0588"
     gene            complement(813..1805)
                     /db_xref="ASAP:ABE-0002027"
                     /db_xref="ECOCYC:EG10298"
                     /gene="fepG"
                     /gene_synonym="ECK0582"
                     /locus_tag="b0589"
     CDS             complement(813..1805)
                     /EC_number="7.2.2.17"
                     /codon_start=1
                     /db_xref="UniProtKB/Swiss-Prot:P23877"
                     /db_xref="ASAP:ABE-0002027"
                     /db_xref="ECOCYC:EG10298"
                     /gene="fepG"
                     /gene_functions="transport (smcogs) SMCOG1011:transport
                     system permease protein (Score: 351.7; E-value: 5.7e-107)"
                     /gene_kind="transport"
                     /gene_synonym="ECK0582"
                     /locus_tag="b0589"
                     /product="ferric enterobactin ABC transporter membrane
                     subunit FepG"
                     /protein_id="AAC73690.1"
                     /transl_table=11
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALMG
                     DAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWSGV
                     LVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVAFNT
                     WLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLEMGDD
                     TACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARWGLTQA
                     ALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(1802..2806)
                     /db_xref="ASAP:ABE-0002031"
                     /db_xref="ECOCYC:EG10296"
                     /gene="fepD"
                     /gene_synonym="ECK0583"
                     /locus_tag="b0590"
     CDS             complement(1802..2806)
                     /EC_number="7.2.2.17"
                     /codon_start=1
                     /db_xref="UniProtKB/Swiss-Prot:P23876"
                     /db_xref="ASAP:ABE-0002031"
                     /db_xref="ECOCYC:EG10296"
                     /gene="fepD"
                     /gene_functions="transport (smcogs) SMCOG1011:transport
                     system permease protein (Score: 339; E-value: 3.9e-103)"
                     /gene_kind="transport"
                     /gene_synonym="ECK0583"
                     /locus_tag="b0590"
                     /product="ferric enterobactin ABC transporter membrane
                     subunit FebD"
                     /protein_id="AAC73691.1"
                     /transl_table=11
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAFS
                     GTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGASFA
                     IVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAAVLE
                     GLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALNSLSL
                     GSDTATALGSRVARTQLIGLLAITVLCGSATAIVGPIAFIGLMMPHMARWLVGADHRWS
                     LPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA"
     gene            2917..4167
                     /db_xref="ASAP:ABE-0002036"
                     /db_xref="ECOCYC:EG11104"
                     /gene="entS"
                     /gene_synonym="ECK0584"
                     /gene_synonym="ybdA"
                     /locus_tag="b0591"
     CDS             2917..4167
                     /codon_start=1
                     /db_xref="UniProtKB/Swiss-Prot:P24077"
                     /db_xref="ASAP:ABE-0002036"
                     /db_xref="ECOCYC:EG11104"
                     /gene="entS"
                     /gene_functions="transport (smcogs) SMCOG1020:major
                     facilitator transporter (Score: 394.4; E-value: 8.9e-120)"
                     /gene_kind="transport"
                     /gene_synonym="ECK0584"
                     /gene_synonym="ybdA"
                     /locus_tag="b0591"
                     /product="enterobactin exporter EntS"
                     /protein_id="AAC73692.1"
                     /transl_table=11
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQIQ
                     MMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLNAL
                     LPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSVISP
                     MIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFRFLLA
                     SPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKL
                     AHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVLV
                     ELRHFRQTPPQVTASDS"
     gene            complement(4171..5127)
                     /db_xref="ASAP:ABE-0002038"
                     /db_xref="ECOCYC:EG10294"
                     /gene="fepB"
                     /gene_synonym="ECK0585"
                     /locus_tag="b0592"
     CDS             complement(4171..5127)
                     /EC_number="7.2.2.17"
                     /codon_start=1
                     /db_xref="UniProtKB/Swiss-Prot:P0AEL6"
                     /db_xref="ASAP:ABE-0002038"
                     /db_xref="ECOCYC:EG10294"
                     /gene="fepB"
                     /gene_functions="transport (smcogs) SMCOG1033:iron compound
                     ABC transporter, periplasmic (Score: 340; E-value:
                     1.8e-103)"
                     /gene_kind="transport"
                     /gene_synonym="ECK0585"
                     /locus_tag="b0592"
                     /product="ferric enterobactin ABC transporter periplasmic
                     binding protein"
                     /protein_id="AAC73693.1"
                     /transl_table=11
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLESQ
                     PQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLYIG
                     EPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLGEIT
                     GHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQMLEQL
                     GFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIYANPLL
                     AHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF"
     gene            5502..6677
                     /db_xref="ASAP:ABE-0002047"
                     /db_xref="ECOCYC:EG10261"
                     /gene="entC"
                     /gene_synonym="ECK0586"
                     /gene_synonym="fepF"
                     /locus_tag="b0593"
     CDS             5502..6677
                     /EC_number="5.4.4.2"
                     /codon_start=1
                     /db_xref="UniProtKB/Swiss-Prot:P0AEJ2"
                     /db_xref="ASAP:ABE-0002047"
                     /db_xref="ECOCYC:EG10261"
                     /gene="entC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) EntC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018:isochorismate synthase (Score: 412.6; E-value:
                     4.1e-125)"
                     /gene_kind="biosynthetic-additional"
                     /gene_synonym="ECK0586"
                     /gene_synonym="fepF"
                     /locus_tag="b0593"
                     /product="isochorismate synthase EntC"
                     /protein_id="AAC73694.1"
                     /sec_met_domain="EntC (E-value: 7.2e-143, bitscore: 465.9,
                     seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAVN
                     GDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEKQA
                     SARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDAAID
                     SGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARRQPDE
                     VLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWHLATPF
                     EGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWCDSEGNG
                     EWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            6687..8297
                     /db_xref="ASAP:ABE-0002049"
                     /db_xref="ECOCYC:EG10263"
                     /gene="entE"
                     /gene_synonym="ECK0587"
                     /locus_tag="b0594"
     CDS             6687..8297
                     /EC_number="6.3.2.14"
                     /NRPS_PKS="Domain: AMP-binding (34-437). E-value: 1.4e-81.
                     Score: 266.0. Matches aSDomain:
                     nrpspksdomains_b0594_AMP-binding.1"
                     /codon_start=1
                     /db_xref="UniProtKB/Swiss-Prot:P10378"
                     /db_xref="ASAP:ABE-0002049"
                     /db_xref="ECOCYC:EG10263"
                     /gene="entE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     448.1; E-value: 5.4e-136)"
                     /gene_kind="biosynthetic-additional"
                     /gene_synonym="ECK0587"
                     /locus_tag="b0594"
                     /product="2,3-dihydroxybenzoate-AMP ligase"
                     /protein_id="AAC73695.1"
                     /sec_met_domain="AMP-binding (E-value: 2.1e-89, bitscore:
                     289.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDG
                     ERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLAL
                     FSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGEHNL
                     QDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQ
                     QTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPP
                     AVSLWLQALIEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVN
                     YTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQ
                     HNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVI
                     YAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTA
                     VGKVDKKQLRQWLASRASA"
     aSDomain        6789..7997
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_b0594_AMP-binding.1"
                     /evalue="1.40E-81"
                     /label="b0594_AMP-binding.1"
                     /locus_tag="b0594"
                     /protein_end="437"
                     /protein_start="34"
                     /score="266.0"
                     /specificity="substrate consensus: diOH-Bz"
                     /tool="antismash"
                     /translation="AASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQ
                     LGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDF
                     LNTFVTEHSSIRVVQLLNDSGEHNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTP
                     KLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLA
                     ADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALIEGESRAQLASLKLLQVGGARLSAT
                     LAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNP
                     LPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKD
                     QINR"
     CDS_motif       7812..7901
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_b0594_0001"
                     /evalue="5.50E-04"
                     /label="NRPS-A_a6"
                     /locus_tag="b0594"
                     /protein_end="405"
                     /protein_start="375"
                     /score="12.4"
                     /tool="antismash"
                     /translation="PQGEVGRLMTRGPYTFRGYYKSPQHNASAF"
     gene            8311..9168
                     /db_xref="ASAP:ABE-0002052"
                     /db_xref="ECOCYC:EG10260"
                     /gene="entB"
                     /gene_synonym="ECK0588"
                     /gene_synonym="entG"
                     /locus_tag="b0595"
     CDS             8311..9168
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /NRPS_PKS="Domain: PP-binding (216-275). E-value: 3e-10.
                     Score: 32.4. Matches aSDomain:
                     nrpspksdomains_b0595_PP-binding.1"
                     /codon_start=1
                     /db_xref="UniProtKB/Swiss-Prot:P0ADI4"
                     /db_xref="ASAP:ABE-0002052"
                     /db_xref="ECOCYC:EG10260"
                     /gene="entB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:isochorismatase (Score: 415.4; E-value:
                     1.2e-126)"
                     /gene_kind="biosynthetic-additional"
                     /gene_synonym="ECK0588"
                     /gene_synonym="entG"
                     /locus_tag="b0595"
                     /product="enterobactin synthase component B"
                     /protein_id="AAC73696.1"
                     /sec_met_domain="PP-binding (E-value: 5.3e-11, bitscore:
                     32.4, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVSF
                     WGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPE
                     QQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTTATD
                     AFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAALREVI
                     LPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLS
                     REVK"
     aSDomain        8959..9135
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_b0595_PP-binding.1"
                     /evalue="3.00E-10"
                     /label="b0595_PP-binding.1"
                     /locus_tag="b0595"
                     /protein_end="275"
                     /protein_start="216"
                     /score="32.4"
                     /tool="antismash"
                     /translation="ALREVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGD
                     IDFVMLAKNPTIDA"
     gene            9168..9914
                     /db_xref="ASAP:ABE-0002054"
                     /db_xref="ECOCYC:EG10259"
                     /gene="entA"
                     /gene_synonym="ECK0589"
                     /locus_tag="b0596"
     CDS             9168..9914
                     /EC_number="1.3.1.28"
                     /NRPS_PKS="Domain: PKS_KR (8-157). E-value: 8.1e-12. Score:
                     37.2. Matches aSDomain: nrpspksdomains_b0596_PKS_KR.1"
                     /codon_start=1
                     /db_xref="UniProtKB/Swiss-Prot:P15047"
                     /db_xref="ASAP:ABE-0002054"
                     /db_xref="ECOCYC:EG10259"
                     /gene="entA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) EntA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     239.6; E-value: 5.9e-73)"
                     /gene_kind="biosynthetic-additional"
                     /gene_synonym="ECK0589"
                     /locus_tag="b0596"
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="AAC73697.1"
                     /sec_met_domain="EntA (E-value: 5.7e-111, bitscore: 359.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQYP
                     FATEVMDVADAAQVAQVCQRLLAETERLDALVNAAGILRMGATDQLSKEDWQQTFAVNV
                     GGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGLELA
                     GSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEIANTI
                     LFLASDLASHITLQDIVVDGGSTLGA"
     CDS_motif       9192..9260
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_b0596_0001"
                     /evalue="6.60E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="b0596"
                     /protein_end="31"
                     /protein_start="8"
                     /score="12.4"
                     /tool="antismash"
                     /translation="WVTGAGKGIGYATALAFVEAGAK"
     aSDomain        9192..9638
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_b0596_PKS_KR.1"
                     /evalue="8.10E-12"
                     /label="b0596_PKS_KR.1"
                     /locus_tag="b0596"
                     /protein_end="157"
                     /protein_start="8"
                     /score="37.2"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="WVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQYPFATEVMDV
                     ADAAQVAQVCQRLLAETERLDALVNAAGILRMGATDQLSKEDWQQTFAVNVGGAFNLFQ
                     QTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALS"
     gene            9917..10330
                     /db_xref="ASAP:ABE-0002057"
                     /db_xref="ECOCYC:EG11105"
                     /gene="entH"
                     /gene_synonym="ECK0590"
                     /gene_synonym="ybdB"
                     /locus_tag="b0597"
     CDS             9917..10330
                     /codon_start=1
                     /db_xref="UniProtKB/Swiss-Prot:P0A8Y8"
                     /db_xref="ASAP:ABE-0002057"
                     /db_xref="ECOCYC:EG11105"
                     /gene="entH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1144:thioesterase superfamily protein (Score: 208.5;
                     E-value: 4.3e-64)"
                     /gene_kind="biosynthetic-additional"
                     /gene_synonym="ECK0590"
                     /gene_synonym="ybdB"
                     /locus_tag="b0597"
                     /product="proofreading thioesterase in enterobactin
                     biosynthesis"
                     /protein_id="AAC73698.1"
                     /transl_table=11
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDTR
                     THQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVCQP
                     LHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
ORIGIN
        1 ttaactattt gcggttgaag gtgccgttcg tccgagcggc accacaagcg gcgttccggc
       61 aactggatcg tcaatgatca tgcagcgcag accataaatg cgctcaatca gttcagcagt
      121 gacaatctcc ttcggcgctc cctgagcaac aattttccct tcccgcaatg caatcaaatg
      181 gctggcgtaa cgacaggcct gattaagatc gtgcagcacc gccgccaggg tatagccttt
      241 ttcgcggttc agttcgctta acaactccag caaatcaatc tgatgactga tatccagcca
      301 ggtcgtgggt tcgtcgagca gcataattgc cgtttcctgg gccagcacca tcgcgatcca
      361 cgctcgctgg cgttgtccgc cagaaagggt atccacgctt tgatctgcca gatgagttat
      421 tcccgtggcc tgcattgctt tcgttaccgc ttcttcatcc tctttgcgcc agcgggtaaa
      481 cagcggttga tgcggataac gtccacgcgc caccagctcc tgcacggtga tatcgcccgg
      541 cgtggtagca ttttgcgcca acagaccaat ccggcgtgca acctctttac tggcgtaatg
      601 ttgaatgtgc tcgccatcca gccagacatg cccatgagca ggcgtcatca ggcggcttaa
      661 ggtacgcagt aacgtggatt taccgcagcc atttggcccg ataattgccg tgaagtgacc
      721 atcaggtatt tctacagtca gattttccgc aacggtatat ttgccatatc ccagggttaa
      781 ctgttcgccg cgcaaacggg ctactgattc ggtcattttt tgcgagactc ctgaattaac
      841 aagacgataa ggtaaatacc gccgaggctg acggtaacga cgccaaccgg aagttgatac
      901 ggcataaaca gttgttgggc gcagagatcg gccgccagca gtaacagcgc cccgcatagc
      961 gccgcctggg ttagccccca gcgagcggtg ccgctaatgc gtcgggcaat gtgcggtgcg
     1021 actaaagcaa taaaggaaat cggcccggca agcgctgttg ccgcagcggt aagcaccact
     1081 gcaaccagca tcattaacag acgcgaacgt tcgacgctga cgcccagcgc acacgcggta
     1141 tcatcgccca tttccagcaa gcgcatccgg cgtaccagta aggcggcggc aatgagcatc
     1201 aatataatga tgggtgcgga aggcgaggtt tttgcccacg tcaggccgtt gagcgatccg
     1261 gcattccaca aacctgctgt tagcgccgtt tctaaagacg ctttcagcaa cagccaggta
     1321 ttaaaggcca ccagcatggc gcgaacgccg ataccgataa taatcaaccg aaaggtgtcg
     1381 atgccgttgc gccaggcgag cagccagacc agcagcgaag tgacaatgcc gcccaccatt
     1441 gctgacagcg cgatagccgt caggtcctga ccaaacagca ccatcgccac cagcacgccg
     1501 ctccacgccc cggtgttaaa gcccattacg tcagggctgc cgagcgggtt acgcatcagc
     1561 gactgaaaaa tcgcgccact gacgcccagt gctgcgccaa tcaacagcgc catcagcacg
     1621 cgtggtaaac gccattcggt gaccaccatc gtcatactgc gcggcgcatc gcccatcagc
     1681 gcggcgaata cctgcgaggt ttccagcgtg acggcaccgc tgcgtaatcc ccagatacct
     1741 gcaaccacac aggcggaaac cagcagcaaa caggtgatga gtaatcggcg agagacgtaa
     1801 atcatgcacc acctcgcgtt ttacgtcgca cgaggaagat cagcaccggt gcaccaataa
     1861 acgcactgac cacagaaacg cgcagttcgc cgggcacaat cacccgcccg atgatatcgg
     1921 caaacagcag cagggcaggg gtagcaagta gcgtgacggg cagcgaccag cgatgatcgg
     1981 cacccaccag ccaacgcgcc atatgcggca tcatcaggcc aataaaggca atcgggccaa
     2041 ctattgccgt cgcactacca caaagcacgg taatcgccag cagaccaatc aactgtgtgc
     2101 gcgccacgcg actgcccagc gccgtcgcgg tgtcgctgcc gaggctcaaa ctgttcagcg
     2161 cgcgactcag taatagcgca gttgctccgg cgatcagcac cgggatcagc accactttta
     2221 aggtatgtag attgcgaata tccagcgaac cggcttgcca gaaacgcaac tgatcgtaga
     2281 cgtcaggatt aagcagggcg atgccgctgg tcagtccttc cagcaccgcc gccagcgcca
     2341 cgcccgccag ggttaaacgc accggactta actgcccgcc gccctgactg ccggtaaagg
     2401 caacaatcaa tgaggccacc agcgccccgg cgaaggccat cgccagttgt tcctgcgcgg
     2461 aagagtaacc aaacagcgcc gcacccagca caatggcaaa gctggctccg gcgttcacgc
     2521 caagcaagcc ggggtcggca agtgggtttc gggtgagggt ttgcattaac gccccggcaa
     2581 ggccaagcgc gccgcctgcc agtaaaccgg caagggtacg cggcagccgc gcgtcgagca
     2641 cgatggtgca gtcggcgctc tggcaggtgc cggagaaggc ctccagcact acggaagcgg
     2701 ggagtgattt tgccccaatg agcaggctta atgccgtcgc gataatcagt aataacagca
     2761 atccgggcac ggcaatggcg cgtgtcacgg caacagaacc agacatacga acttccatga
     2821 taatgaaatt aattatcgtt atcgatctta tttggatatg ttagcatgtg cagcctaaga
     2881 ataggtattt aaaatatttg atggcaaggc attgtaatga ataaacaatc ctggctgctt
     2941 aacctcagcc tgttgaaaac gcacccggcg tttcgcgcag tattcctcgc tcgtttcatc
     3001 tcaattgtgt ctctgggttt gctcggcgtc gcggtgccgg tgcagatcca gatgatgaca
     3061 cattccacct ggcaggtggg gctttcggtg acgctgaccg gcggcgcgat gtttgttggc
     3121 ctgatggtcg gcggtgtgct ggcggatcgc tatgagcgca aaaaagtgat tttgctggcg
     3181 cgcggcacct gtggcattgg cttcattgga ctgtgcctta atgcactgct gccggagccg
     3241 tcattgctgg caatctattt acttggttta tgggatggtt ttttcgcatc gcttggcgtt
     3301 acggcgctat tggcggcgac accagcactg gtagggcgtg aaaacttaat gcaggccggg
     3361 gcgatcacca tgttgaccgt gcgtctgggg tcggtgattt cgcccatgat tggcggttta
     3421 ttgctggcga ccggtggcgt agcctggaac tacgggctgg cggcggcggg cacgtttatt
     3481 accttgctac cgttgttaag ccttccggcg ttgccaccgc caccgcagcc gcgtgagcat
     3541 ccgttgaaat cattactggc aggatttcgt tttctgcttg ccagcccgct ggtgggcggg
     3601 attgcgctgc tgggtggttt attgacgatg gcgagcgcgg tgcgggtact gtatccggcg
     3661 ctggctgaca actggcagat gtcagcggca cagattggtt ttctctacgc ggcgatcccg
     3721 ctcggcgcgg ctattggtgc gttaaccagc gggaagctgg cacatagtgc gcgaccaggg
     3781 ttattgatgc tgctctccac gctgggatcg ttcctcgcca ttggtctgtt tggcctgatg
     3841 ccgatgtgga ttttaggcgt ggtttgtctg gcgctgttcg gctggttgag tgcggtcagc
     3901 tcgttgctgc aatacacaat gctgcaaacg caaaccccgg aagcgatgtt agggcggatt
     3961 aacggtttgt ggacggcgca gaacgtgacg ggcgatgcca taggcgcggc gctgctgggt
     4021 ggtttgggcg cgatgatgac accggttgct tccgcaagcg cgagcggttt tggtttgttg
     4081 attatcggcg tgttgttatt gctggtgctg gtggagttgc gacattttcg ccagacgccg
     4141 ccgcaggtga cagcgtccga cagttaatgc ttaaaacagc gccttaagcc tatccagcac
     4201 ttgcatggcg ctgtagtaat ccagacggaa cgtctcggtt cccagcgcat aaacctgctt
     4261 gttttgtact gcaggcaggt gcgcgagcag cggattagca taaatagcat cggcatcttt
     4321 ctgatcaccg gcgaacagga atagtgactc gccatttaac cctgcagcca gattttcccc
     4381 accaagctga atgatgtcat ggcgtttacc ctgactttgg ctggcattta accctgcggg
     4441 taacttcgcc agcgtaaagc cgagttgttc cagcatctgc ccttgtgctg attctggcgt
     4501 ccagagattg gcactgtgtg cagcggcagt atagacaatg gcagtgaccg gctgcggcgg
     4561 taatttgatt tgctctttcg ccgccgccag ttgcttatca aactgcgcaa tccgctctgc
     4621 cgcttgtttc tcatgcccgg taatttcgcc aagttgcgtt aacagcgact gccagctttt
     4681 gtcgtcgtaa ttgatgatta atgtcggggc gatggtggaa agctgatcat acagtgccag
     4741 cgccgaatcc ccgccggttg cgctaattaa aatcagatcc ggcatttgcg cggcaacggc
     4801 ttcggcgctc ggttcgccga tatagagccg ttgcagtttg cgttctttcg ccaccttgct
     4861 ccactggcgt aaaaagccct ggtcatccgc gacgcggtta ttcggcgtgg tcgcgccgct
     4921 ggcgatcacc ggagcatcaa tcgccagcag tgagccggtc agggtgacgc tggtggaaac
     4981 aatacgctgc ggctggcttt ccagtgtatg tgtgccacgg ctgtcagtaa tctgacgcgg
     5041 ccagtcagcg gcctgaactg cggctattcc tgaaagcaaa agtcctgtta atagaagggc
     5101 gttgcggtag agcggggcga gtctcacaaa tcagcttcct gttattaata aggttaaggg
     5161 cgtaatgaca aattcgacaa agcgcacaat ccgtcccctc gcccctttgg ggagagggtt
     5221 agggtgaggg gaacagccag cactggtgcg aacattaacc ctcaccccag ccctcaccct
     5281 ggaagggaga gggggcagaa cggcgcagga catcacattg cgcttatgcg aatccatcaa
     5341 taatgcttct cattttcatt gtaaccacaa ccagatgcaa ccccgagttg cagattgcgt
     5401 tacctcaaga gttgacatag tgcgcgtttg cttttaggtt agcgaccgaa aatataaatg
     5461 ataatcatta ttaaagcctt tatcattttg tggaggatga tatggatacg tcactggctg
     5521 aggaagtaca gcagaccatg gcaacacttg cgcccaatcg ctttttcttt atgtcgccgt
     5581 accgcagttt tacgacgtca ggatgtttcg cccgcttcga tgaaccggct gtgaacgggg
     5641 attcgcccga cagtcccttc cagcaaaaac tcgccgcgct gtttgccgat gccaaagcgc
     5701 agggcatcaa aaatccggtg atggtcgggg cgattccctt cgatccacgt cagccttcgt
     5761 cgctgtatat tcctgaatcc tggcagtcgt tctcccgtca ggaaaaacaa gcttccgcac
     5821 gccgtttcac ccgcagccag tcgctgaatg tggtggaacg ccaggcaatt ccggagcaaa
     5881 ccacgtttga acagatggtt gcccgcgccg ccgcacttac cgccacgccg caggtcgaca
     5941 aagtggtgtt gtcacggttg attgatatca ccactgacgc cgccattgat agtggcgtat
     6001 tgctggaacg gttgattgcg caaaacccgg ttagttacaa cttccatgtt ccgctggctg
     6061 atggtggcgt cctgctgggg gccagcccgg aactgctgct acgtaaagac ggcgagcgtt
     6121 ttagctccat tccgttagcc ggttccgcgc gtcgtcagcc ggatgaagtg ctcgatcgcg
     6181 aagcaggtaa tcgtctgctg gcgtcagaaa aagatcgcca tgaacatgaa ctggtgactc
     6241 aggcgatgaa agaggtactg cgcgaacgca gtagtgagtt acacgttcct tcttctccac
     6301 agctgatcac cacgccgacg ctgtggcatc tcgcaactcc ctttgaaggt aaagcgaatt
     6361 cgcaagaaaa cgcactgact ctggcctgtc tgctgcatcc gacccccgcg ctgagcggtt
     6421 tcccgcatca ggccgcgacc caggttattg ctgaactgga accgttcgac cgcgaactgt
     6481 ttggcggcat tgtgggttgg tgtgacagcg aaggtaacgg cgaatgggtg gtgaccatcc
     6541 gctgcgcgaa gctgcgggaa aatcaggtgc gtctgtttgc cggagcgggg attgtgcctg
     6601 cgtcgtcacc gttgggtgag tggcgcgaaa caggcgtcaa actttctacc atgttgaacg
     6661 tttttggatt gcattaagga gcgaggatga gcattccatt cacccgctgg ccggaagagt
     6721 ttgcccgtcg ctatcgggaa aaaggctact ggcaggattt gccgctgacc gacattctga
     6781 cgcgacatgc tgcgagtgac agcatcgcgg ttatcgacgg cgagcgacag ttgagttatc
     6841 gggagctgaa tcaggcggcg gataacctcg cgtgtagttt acgccgtcag ggcattaaac
     6901 ctggtgaaac cgcgctggta caactgggta acgtcgctga attgtatatt acctttttcg
     6961 cgctgctgaa actgggcgtt gcgccggtgc tggcgttgtt cagccatcag cgtagtgaac
     7021 tgaacgccta tgccagccag attgaacccg cattgctgat tgccgatcgc caacatgcgc
     7081 tgtttagcgg ggatgatttc ctcaatactt tcgtcacaga acattcctcc attcgcgtgg
     7141 tgcaactgct caacgacagc ggtgagcata acttgcagga tgcgattaac catccggctg
     7201 aggattttac tgccacgcca tcacctgctg atgaagtggc ctatttccag ctttccggtg
     7261 gcaccaccgg cacaccgaaa ctgatcccgc gcactcataa cgactactac tacagcgtgc
     7321 gtcgtagcgt cgagatttgt cagttcacac aacagacacg ctacctgtgc gcgatcccgg
     7381 cggctcataa ctacgccatg agttcgccag gatcgctggg cgtctttctt gccggaggaa
     7441 cggttgttct ggcggccgat cccagcgcca cgctctgttt cccattgatt gaaaaacatc
     7501 aggttaacgt taccgcgctg gtgccacccg cagtcagcct gtggttgcag gcgctgatcg
     7561 aaggcgaaag ccgggcgcag cttgcctcgc tgaaactgtt acaggtcggc ggcgcacgtc
     7621 tttctgccac ccttgcggcg cgtattcccg ctgagattgg ctgtcagttg cagcaggtgt
     7681 ttggcatggc ggaagggctg gtgaactaca cccgacttga tgatagcgcg gagaaaatta
     7741 tccataccca gggttaccca atgtgtccgg atgacgaagt atgggttgcc gatgccgaag
     7801 gaaatccact gccgcaaggg gaagtcggac gcctgatgac gcgcgggccg tacaccttcc
     7861 gcggctatta caaaagtcca cagcacaatg ccagcgcctt tgatgccaac ggtttttact
     7921 gttccggcga tctgatctct attgatccag agggttacat caccgtgcag gggcgcgaga
     7981 aagatcagat taaccgtggc ggcgagaaga tcgctgccga agagatcgaa aacctgctgc
     8041 tgcgccaccc ggcggtgatc tacgccgcac tggtgagcat ggaagatgag ctgatgggcg
     8101 aaaaaagctg cgcttatctg gtggtaaaag agccgctgcg cgcggtgcag gtgcgtcgtt
     8161 tcctgcgtga acagggtatt gccgaattta aattaccgga tcgcgtggag tgtgtggatt
     8221 cacttccgct gacggcggtc gggaaagtcg ataaaaaaca attacgtcag tggctggcgt
     8281 cacgcgcatc agcctgaagg agagaacacg atggctattc caaaattaca ggcttacgca
     8341 ctgccggagt ctcacgatat tccgcagaat aaagttgact gggcctttga accgcaacgt
     8401 gccgcgttgt taatccatga tatgcaggac tattttgtca gcttctgggg cgagaactgc
     8461 ccgatgatgg agcaggtgat cgcgaatatt gctgcgctgc gcgactactg caaacagcac
     8521 aatatcccgg tttattacac cgcccagccg aaagagcaga gcgatgaaga tcgggcgctg
     8581 ttgaatgata tgtgggggcc gggcctgacc cgctcgccgg aacagcaaaa ggtggtggat
     8641 cgcctgacgc cagatgccga cgacacggtg ctggtgaagt ggcgctacag cgcgtttcat
     8701 cgttctccgc tggagcaaat gctgaaagag agtggacgta accagctgat tattaccggg
     8761 gtatatgccc acattggctg tatgaccacc gcaaccgacg catttatgcg cgatattaaa
     8821 ccgtttatgg tggcggatgc gctggccgat ttcagccgtg acgagcattt gatgtcgctg
     8881 aaatatgtgg ccggacgttc tggccgggtg gtgatgactg aagaattact gccagcacct
     8941 atccccgcca gcaaagcggc gctgcgtgag gtgatcctgc cgttgctgga cgagtccgat
     9001 gaaccgttcg atgacgacaa cctgatcgac tacggtctgg attcggtgcg catgatggcg
     9061 ctggcggcgc gctggcgcaa agtgcatggt gatatcgact ttgtcatgct ggcgaaaaac
     9121 ccgaccatcg acgcctggtg gaagctactc tcccgcgagg tgaaataatg gatttcagcg
     9181 gtaaaaatgt ctgggtaacc ggcgcaggta aaggtatcgg ctacgccacg gcgctggcgt
     9241 ttgttgaggc gggagcgaaa gttacaggtt ttgatcaagc gttcactcag gagcaatatc
     9301 cctttgcgac cgaagtgatg gatgttgccg acgctgcgca ggtcgcgcaa gtgtgtcagc
     9361 gactgttagc tgaaacggag cgactggacg cgctggtcaa tgcggcggga attttacgca
     9421 tgggcgcgac cgatcagctc agtaaagagg actggcagca gacttttgcg gttaacgtcg
     9481 gcggtgcgtt taacctgttc cagcaaacca tgaaccagtt tcgccgtcag cggggcgggg
     9541 cgattgtcac tgtggcgtcc gacgccgcgc acacgccgcg tattggcatg agtgcttatg
     9601 gcgcatcgaa agcggcgctg aaaagcctgg cgttgagcgt cgggctggaa ctggcgggta
     9661 gcggcgtgcg ctgtaatgtg gtttcgcctg gctccaccga caccgatatg caacgcacgc
     9721 tgtgggtgag cgatgacgcc gaagaacagc gtattcgcgg ctttggcgag cagtttaaac
     9781 tcggcattcc gctggggaaa atcgcccgtc cacaagagat cgccaacacg attttgttcc
     9841 tcgcctctga cctcgccagc catattaccc tacaggatat tgtggtcgat ggcggctcaa
     9901 cgctgggggc ataagcatga tctggaaacg ccatttaacg ctcgacgaac tgaacgccac
     9961 cagcgataac acaatggtgg cgcatctggg aattgtgtat acccgtctgg gcgatgatgt
    10021 gctggaagcc gaaatgccgg ttgatacccg tactcatcag ccgttcggtt tactacatgg
    10081 cggcgcgtcg gcggcgctgg cggaaacgct gggatcgatg gccggattta tgatgacccg
    10141 cgacggacag tgtgtggtag gcacagaact taatgcaaca caccatcgcc cggtgtctga
    10201 gggaaaggta cgcggcgtct gccagccgct gcatcttggt cggcaaaatc agagctggga
    10261 aatcgtcgtt ttcgatgaac aggggcggcg ttgctgcact tgtcggctgg gtacggcagt
    10321 tttgggatga
//
