LOCUS       BGC0002485             14448 bp    DNA     linear   BCT 07-NOV-2019
DEFINITION  Desmonostoc sp. PCC 7906 muscoride biosynthetic gene cluster,
            complete sequence.
ACCESSION   BGC0002485
VERSION     BGC0002485
KEYWORDS    .
SOURCE      Desmonostoc sp. PCC 7906 (Nostoc sp. PCC 7906)
  ORGANISM  Desmonostoc sp. PCC 7906
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae;
            Desmonostoc.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:48:47
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14448
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..14448
                     /db_xref="taxon:1181"
                     /isolation_source="freshwater"
                     /mol_type="genomic DNA"
                     /organism="Desmonostoc sp. PCC 7906"
                     /strain="PCC 7906"
     protocluster    1..14448
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[7584:11352]"
                     /cutoff="20000"
                     /detection_rule="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /neighbourhood="10000"
                     /product="cyanobactin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      7585..11352
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /neighbourhood="10000"
                     /product="cyanobactin"
                     /protocluster_number="1"
     cand_cluster    1..14448
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="neighbouring"
                     /product="cyanobactin"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     subregion       1..14448
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002485"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14448
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /contig_edge="True"
                     /product="cyanobactin"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     protocluster    1..11493
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[4147:6493]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      4148..6493
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="2"
     cand_cluster    1..11493
                     /candidate_cluster_number="2"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="2"
                     /tool="antismash"
     CDS             144..827
                     /codon_start=1
                     /product="putative restriction endonuclease"
                     /protein_id="QGA72582.1"
                     /transl_table=11
                     /translation="MISTKTNAQLLMIPPLENGDKLTRAEFERRYQAMPNLKKAELIER
                     VVYVPSPLRIKSHGEPHAYIMAWLGTYKAATPGVGFADNTTVLLDADNEPQPDALLRIE
                     QGGQTRITEDDYLEGAPELIVEIAASSASYDLHEKLKVYRRNGVQEYLVWRVYDRQFDW
                     FRLNEGEYIKLDSNGNGVICSQVFPGLWLDKAALLSGDLAKVLAILQQGLSTPEHQSFV
                     EKLSI"
     misc_feature    144..14387
                     /note="muscoride biosynthetic gene cluster"
     gene            complement(1104..2009)
                     /gene="musC"
     CDS             complement(1104..2009)
                     /codon_start=1
                     /gene="musC"
                     /product="MusC"
                     /protein_id="QGA72583.1"
                     /transl_table=11
                     /translation="MNNPPESLQSVLVFNGQDDSINLGNKLEFKIEKQITLEAWVYCQN
                     QRRRTGIVSNVFDTAAQESGYGLLLDGKSGIFFALKTASQGIHYLSSKVDSLKLNQWHH
                     LAGTYDGKQIKVYIDGVEKATKTASNSPINYEPENDLFIGTYKDNNETYPFQGKIAEVR
                     IWKVARTQQEIQNNLYQRLGGNEPGLIGYWPLNEGSNNIAHDGTTNANHGTINGANWEQ
                     SQVPFTSPQSHELLVAKTITKENETTSELREAEQKTKEIEKHLLATGLEDYGFWWQEML
                     KAQAQKTEPDKPFRRGRIWT"
     gene            complement(2315..3700)
                     /gene="musG"
     CDS             complement(2315..3700)
                     /codon_start=1
                     /gene="musG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_kind="biosynthetic-additional"
                     /note="oxazoline oxidase"
                     /product="MusG"
                     /protein_id="QGA72584.1"
                     /sec_met_domain="PF00881 (E-value: 1.5e-15, bitscore: 47.2,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTFTISLANKIDLIPESERIILKSNNYKLTLSHLQPGLKSAFN
                     HLKHSPATLSDLRQLVVETDGIEGKDKFDKYLQGLTNLGWICHSVYPLATAIPMTGNYQ
                     FNYPKIEDWQQQTFTLSRFGYLHQVNGEMILESPQSQAKIILLDWRATALITKLSQPQT
                     STTLIAGIPDITEELAQQFLSLLLATQMLSSETENSHLIQWEFHDLLFHSRSRKGRHSN
                     PIGGTYRFLDKIEPLPVVKPPMSDKVIELYKPDLEDLLKTDVSFTQVLESRRSIREYGE
                     NPITIEQLGELLYRAARIQEIIDKEKMGQLSRRPYPGGGAIHELEIYPLINYCQGIEPA
                     LYHYRPDAHELELVSEWSQEIEALVTEACLSNGSQKMPQILIIITARFNRMFWKYQSMA
                     YAAILKHVGVLYNTLYLVSTAMNLAPCGIGAGNSDIFAKATGIDYYEESSVGEFILGTK
                     KSS"
     gene            complement(4148..6493)
                     /gene="musD"
     CDS             complement(4148..6493)
                     /codon_start=1
                     /gene="musD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Cyanobactin_D_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Goadsporin_CD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NHLP_CD_RRE"
                     /gene_kind="biosynthetic"
                     /note="cyclodehydratase"
                     /product="MusD"
                     /protein_id="QGA72585.1"
                     /sec_met_domain="Cyanobactin_D_RRE (E-value: 1.9e-31,
                     bitscore: 97.6, seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="Goadsporin_CD_RRE (E-value: 1.3e-17,
                     bitscore: 53.4, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="NHLP_CD_RRE (E-value: 9.6e-22, bitscore:
                     66.5, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 6.5e-94, bitscore: 304.8,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 8.6e-85, bitscore:
                     274.2, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKILQIKPHFRAEIIEPKHVYLLSESSTHALTGELYCQLIPLLNG
                     NYTVDEIINKLQVDPSHIDYALERLQARGYITEAIPQLTPEAVAFWGLLKVEPQVAYQC
                     LQQTQVYVSSVVNLPTQPLITALEEVGIKAINWDGELQEFPPHSLLVVLTDDYLQPQLN
                     KINQIALKANQPWLLIKPVGTILWLGPIFQPQITGCWECLAQRLRVNREVEASVLRQKN
                     SSLQLSPSQELNSSVLQSNGNGVKSEVIECLPPPAAVIPSTLQTALHLATTEIAKWIVK
                     QGVEDTTPFPTLEGKVITFDQRNLDLQTHILSLRPQCPSCGNPNLLTERAFQPLVLSSR
                     KKQFTSDGGHRAFSPDQTVNRYQHLISPITGVVTSLVRASDPNDSLNHTYNAVHSFVIA
                     SNIGRMRRYLKHKSSGKGKTDSQSKASGFCEAIERYSGVYQGDEPRISATLAELGEKAI
                     HPARCSLFSSEQYEYREEFNRRGGVFDWIPQPFDETKVIEWTPVWSLTEQTHKYIPTAY
                     CYYGYPLPEDHEFCRANSNGDATGNTLEEAIIQGFFEIVERDSVAIWWYNRLKRPAVDL
                     ASFNEPYLLEVQDLYRSNNRDLWVIDITADLDIPTFVAVSYLKDNKHQTILLGFGTHFD
                     PKIAILRAVTEVNQIAFTCDGVEVTKEFVEMREWFKKATIENQPYLVPDSTVPAKVYQD
                     YQQRWSDDIYEDVMTCVEISKNAGLETLVLDKTRPDIGLNVAKVIVPEMPHYWLRMGAK
                     RIYDVPVKMGWLSTPLTEEQMNPISVPI"
     gene            complement(6904..7104)
                     /gene="musE"
     CDS             complement(6904..7104)
                     /codon_start=1
                     /gene="musE"
                     /note="muscoride precursor peptide"
                     /product="MusE"
                     /protein_id="QGA72586.1"
                     /transl_table=11
                     /translation="MKPENQKQTRLQPKLSEPTVRVMPGIQLDLSEEELTNGGVGVGGV
                     NASQYGGGDGGVSPSVPTTGV"
     gene            7585..9705
                     /gene="musA"
     CDS             7585..9705
                     /codon_start=1
                     /gene="musA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 163.9; E-value: 1.2e-49)"
                     /gene_kind="biosynthetic"
                     /note="N-terminal protease"
                     /product="MusA"
                     /protein_id="QGA72587.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 2.5e-38, bitscore:
                     121.5, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 2.5e-220,
                     bitscore: 721.4, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKVKIVPGLTHLWEQTLGKPEICVAVLDGLVDRSHVCFQGADLR
                     EIPGMVESIPNPEGLMHRHGTHVASVIFGQHGSPVEGIAPRCKGLSIPIFSDKGRSLSQ
                     LDLARAIEQAVNSGAHIINLSGGQLADYGQADGWLEKAVALCKENNVLLVAAAGNNGCE
                     CLHVPAALPAVLAVGAMDDGGQPIDFSNWGDTYQTQGILAPGENILGARAGGGVKEWTG
                     TSFATPIVSGVAALLLSLQQQRGEKPDPQKVRDAILQSAIPCELKQQGDRPRCLVGKLN
                     ILGAMEKLKGGKMSQQPEALTASGCGCGGVSKGDSLYPINYMNHSAVEDSGTSPSELGS
                     SEFDGVTESAPGEALASVGGATSHPISTEAPSTSNSNVASNVSLPTSADLAAFQNLLQS
                     YITTSNQPMSYPVASSVTASQAPSDINPETLVYALGTLGYDFGTEARRDSFKQLMPGIS
                     IDNTGVPANPYDARQMVDYLADNPSEGKSLIWTLNLELTPIYAIEPKGAFARDVYGVLQ
                     ELLSGQIQPEDDPEYIERVSIPGLLTGRTVKLFSGQYIPVIEPQNTRGLYGWKVNTLIN
                     AALDTINAQVGEPQEENVRRTLGGFLNRIYYDFRNLGTTSQDRALNFATTNAFQAATTF
                     AQAVAEQMELDTITVERSPFCRKDSDCWDVKLKFFDPENNRRAKKIFRFTIDVSDLIPV
                     TLGEVRTWSSAY"
     gene            9718..11352
                     /gene="musH"
     CDS             9718..11352
                     /NRPS_PKS="Domain: oMT (46-212). E-value: 2.4e-25. Score:
                     80.4. Matches aSDomain: nrpspksdomains_musH_oMT.1"
                     /codon_start=1
                     /gene="musH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 127; E-value: 1.4e-38)"
                     /gene_kind="biosynthetic"
                     /note="methyltransferase"
                     /product="MusH"
                     /protein_id="QGA72588.1"
                     /sec_met_domain="cyanobactin_synth (E-value: 1.6e-154,
                     bitscore: 504.5, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQFLTSNQTHAPPDNHWLKQRQLYNLARFNQNKDITDSWVRSHNL
                     NLGKQAGIKPGNYILDAGCGAGIPALQIAQEFPGIRVEGITPSEIQAQDARQRVQASGL
                     GDRIQIRVGDFHYLPFPNGVFDVAFFNESIKYSKNLPQALAEVYRVLRPGGHLYITDLV
                     SQELPLTEQQQRELAKLNQDLICHIYSLSQLAKAGQQAGFQNIEFGDFTKHLPAGDMRK
                     LWLLNPAANMPVYYGEIKASKGVASQITPSQKLEVPVQTRTSRLVYALGTVGYDFGSQA
                     RRDSFKQLMPGIAIDGTVIPANPYDARQVVDYLVDNPPEAKSLIWTLNLDLTPIYAIEP
                     IEAFADDVYETLQLLLAGQIEPQDSDNYIERASIPARLTNRTVQLFSGQIVPVIEPENI
                     RGMYGWKINSLVDAALETLATETETIEAQRARVQRTLTSFLNRIYYDLRNWGQTDRDRA
                     LNFAATNAFQAASTFKEAVAAGLELDSIEIYKSAYCRFDSNCWDVKLKFFDPESNRRAR
                     KIFRFTIDVREIMPVTLGEVRSWAAPK"
     aSDomain        9856..10353
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_musH_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="2.40E-25"
                     /label="musH_oMT.1"
                     /locus_tag="musH"
                     /protein_end="212"
                     /protein_start="46"
                     /score="80.4"
                     /tool="antismash"
                     /translation="LGKQAGIKPGNYILDAGCGAGIPALQIAQEFPGIRVEGITPSEIQ
                     AQDARQRVQASGLGDRIQIRVGDFHYLPFPNGVFDVAFFNESIKYSKNLPQALAEVYRV
                     LRPGGHLYITDLVSQELPLTEQQQRELAKLNQDLICHIYSLSQLAKAGQQAGFQNIEFG
                     DFT"
     gene            11393..11593
                     /gene="musB"
     CDS             11393..11593
                     /codon_start=1
                     /gene="musB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) triceptide_precursor"
                     /gene_kind="biosynthetic-additional"
                     /product="MusB"
                     /protein_id="QGA72589.1"
                     /sec_met_domain="triceptide_precursor (E-value: 1.0,
                     bitscore: 10.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLPKLAPPVKRPDIIYPHRSVDVINGRVEDLVHIRMDLLHGANY
                     NDPPAFNYSACSQSGYGCGGW"
     gene            12233..13135
                     /gene="musF1"
     CDS             12233..13135
                     /codon_start=1
                     /gene="musF1"
                     /note="muscoride prenyltransferase"
                     /product="MusF1"
                     /protein_id="QGA72590.1"
                     /transl_table=11
                     /translation="MVTYKPIQTDSEPYLEYIGKHKRAFDIYEDLYPVKLFEDFVQVEG
                     RQGGMFRCGCNVTKDELSPARFALFFPTQDDLQLLHNPQQQLEAAFDFFRKVEGRAEVK
                     VNYHLIEKFFGKNPDFRGIYFIAVGVDAQTEIAESRLKLFIHLSNAPDKIESAIALCGD
                     SPTLRAFLVNNDLLVGFDFYLDGRSEIEIYPTIYQEELERADIQSRILPLLPPRALPLL
                     KQCEVLQVGFSPANKSNILYFDFVSDPNTFIDNLGNEMAKKVHAYYRHQPVKHVLVGIS
                     EKDLYASSIEKVKLYYFKK"
     gene            13192..14103
                     /gene="musF2"
     CDS             13192..14103
                     /codon_start=1
                     /gene="musF2"
                     /note="muscoride prenyltransferase"
                     /product="MusF2"
                     /protein_id="QGA72591.1"
                     /transl_table=11
                     /translation="MVTYKPIPTDSERYLEYIRKHQLAFDIYEDLYPLKLFEDFVQAEG
                     RKESWFYNCCNIDKDQLYPARFTFGYPPQKEAKLLHNPQRQLQEVFHFFRQVENRPEVK
                     LNYRFIHQFFSIHPDVRGICWVGVGVDTRTVITESRLKLFIGLKDAPDKVETAITLCGD
                     SPTLRAFLLNDYNNYLLIGFDLFFDNHSEIELYPTIFEDKLRLSNVRSQITPLLPPRAL
                     PLLDQCEVFQIGISPANDSNILYFDDVYDPNNFVDNFGNEMVKKVHAYYRHQPIKKLLI
                     GAPEKDFYAQSIEHAKLYYRMN"
     gene            14175..14387
                     /gene="musC2"
     CDS             14175..14387
                     /codon_start=1
                     /gene="musC2"
                     /product="MusC2"
                     /protein_id="QGA72592.1"
                     /transl_table=11
                     /translation="MAKKQQTPENSEPTPITPPIKQPEGIPKQIESHLLATGLEDYGFW
                     WQEMLKAQANSDKERKPFRRGRIWS"
ORIGIN
        1 taagccaatt ttttaatagt aaaattatta atatagcaat tcccagctaa attaggaaat
       61 aaagcagaaa ccttctctgt ctgttgccta ttgcctattc cctgttccct acaatatcag
      121 taattaacca tcatcaaaaa attatgatat ctactaagac aaatgctcaa ctgttaatga
      181 ttccgccttt agaaaatggc gacaaactca cccgtgctga atttgagcgc cgttatcaag
      241 caatgccaaa tctgaaaaaa gcagaattga ttgaaagagt tgtttacgtg ccatccccat
      301 taagaatcaa gagtcatgga gaaccccatg cttacatcat ggcttggctt ggaacttaca
      361 aagcagcgac accaggcgta ggttttgcag ataataccac tgttttatta gatgctgata
      421 acgaaccgca accagatgct ttattaagaa tagaacaagg tggacaaact cgaattactg
      481 aagatgatta tttagaaggt gcgccagaat tgattgtaga aattgctgct tctagtgctt
      541 cctatgattt gcatgaaaag ctcaaagttt atcgccgtaa tggagtacag gaatatttag
      601 tgtggcgagt ttatgaccgt caatttgatt ggtttaggtt aaacgaaggg gaatatatta
      661 aacttgattc caatggtaat ggtgtaatat gctcacaagt atttccagga ttatggttag
      721 ataaagcggc tttattatcg ggagatttag ctaaagtttt agccatttta caacagggat
      781 tatctacacc agaacatcaa agttttgttg aaaaattatc tatttagaca gatatatctc
      841 tgatacacca aaattcagaa ttttgatata cttaggactt acgcaaactc tacgaactct
      901 tggcgttctt ctctaacgag acgctccgcg ttggcgttgg cgttggcgaa gcctctcgta
      961 gagaagcctc tcgtagagaa gcctctcgca gagaaggcgt cttctctacg agacgcgaag
     1021 cgcgaaggcg gtagcctacg gcaagccgca aagcgtctac gataaattag gcgaagttgg
     1081 ctatttttgc gtaagtccta atattaagtc caaattcgac cgcgacgaaa aggtttatct
     1141 ggctcagttt tttgagcctg cgctttcaac atttcttgcc accaaaatcc atagtcttcg
     1201 agtccagtag ctaaaagatg tttttcaatc tctttcgttt tctgttctgc ttcccttaac
     1261 tctgaagtag tttcattttc cttggttatg gttttggcaa ctagtaactc atgagattga
     1321 ggactagtaa agggcacttg tgactgttcc cagttagcac cattaatagt accatgattg
     1381 gcattggtag taccatcatg agctatattg ttactgcctt catttaaagg ccaataacca
     1441 attaatcccg gttcatttcc tccaagacgt tggtagagat tattttgaat ttcttgttgg
     1501 gtacgagcta ctttccaaat gcgaacttca gcaattttac cttgaaacgg ataggtttcg
     1561 ttattatctt tatacgtccc gataaataaa tcattttcgg gttcgtaatt aattggagag
     1621 ttggaagctg ttttagtggc tttttctacg ccgtcaatat aaacttttat ctgtttacca
     1681 tcgtaggttc cagctagatg atgccattga ttgagtttta aactatcaac cttactgcta
     1741 agataatgaa ttccttggga tgcagttttc aaggcaaaaa atatcccact cttaccatcg
     1801 agaagcagac catatccact ttcttgagca gctgtgtcaa aaacattaga aacaatacca
     1861 gttcgtcgtc gttgattttg acaataaacc caagcttcta aagtaatttg cttctcgatt
     1921 ttaaattcta gtttgttgcc taaatttatt gagtcgtctt gtccattaaa tacaaggact
     1981 gattgtaatg attctggtgg gttattcata tcaaaattgg agattttttg tataaggcaa
     2041 tatgcttgat ttaataccaa tttaatttga agttgcacat atcttaatcc ccctaaatcc
     2101 cccttaaaaa gggggacttt gatagatgtt ttccggttcc ccccttatta aggggggtta
     2161 ggggggatcg aattctatgc agctttataa aaaattggta taaggtcaaa actttttata
     2221 aaaaaagatc taattaactg ttgtggactg ggcgggcgag acgcccaccc cacaagattg
     2281 aataatttat tttttggaaa cttcgcaaac tatatcacga agattttttt gtacctagta
     2341 taaactcccc aacagaagat tcttcatagt aatcaattcc tgttgctttg gcaaatatat
     2401 cggaattacc agcacctatt ccacagggag ctaaattcat tgcggttgat actaaataaa
     2461 gagtgttata taaaactcca acgtgtttta aaattgcagc gtaggccatt gattggtatt
     2521 tccaaaacat gcggttgaat ctcgcagtaa ttataattaa aatttgcggc attttctggc
     2581 taccattaga taaacaagct tctgtgacta gagcttctat ttcttgactc cactcagaaa
     2641 ctaactctaa ttcatgggca tcaggacgat aatgatatag tgcaggttct attccttggc
     2701 agtagttaat cagtggataa atctctaact catgaatagc accgcctcct ggataaggac
     2761 gacggcttaa ttgtcccatt ttttctttat caattatttc ttggattcta gcagcgcgat
     2821 atagtaattc tcctaattgt tctatggtga ttggattttc accatactct cgaattgaac
     2881 gtctcgattc taatacttga gtgaaagaaa catctgtttt taataaatct tctaggtcgg
     2941 gtttatataa ttcaattacc ttgtcgctca tgggtggttt taccacaggt agaggttcaa
     3001 ttttatctaa gaaacgatat gttcccccga ttgggttact atgtctacct tttcggctgc
     3061 gactgtggaa gaggaggtca tgaaattccc attggatgag atgagaattt tcggtttctg
     3121 aagatagcat ttgggtggct aaaagtagac tgagaaactg ttgtgctagt tcttctgtga
     3181 tgtctggaat tcctgcgatt aatgttgtgc tggtttgggg ctgagataac ttagtaatta
     3241 gggctgttgc tcgccaatct aataaaatta ttttggcttg agactgggga gattctaaaa
     3301 tcatttcccc gtttacttgg tgcaaatagc caaaccgaga tagagtgaat gtttgttgtt
     3361 gccaatcttc tatttttgga taattaaact gatagtttcc cgtcatggga atagcagtcg
     3421 ccaggggata aactgaatga caaatccagc ctaagttagt tagtccttgc aaatatttat
     3481 caaacttatc ttttccttca attccatcag tttctacgac caattgtctc aggtcagaga
     3541 gagtagcggg ggaatgcttg agatggttga atgcagattt taaccctggt tgtaggtgag
     3601 ataaagttag tttgtaatta ttagatttga gaatgatgcg ctcactctct ggaattaggt
     3661 caattttgtt tgctaaggaa atggtgaaag ttgagttcat gggattaacg aaaatagtaa
     3721 gtaaaaatta aatagtttgt agtaaggact ttagtcctca agccaggact aaagtcctca
     3781 ctacgagcaa acatttgttt tgtttaggga tttcaaaata agaatattca attatttctt
     3841 gtggggtggg catcttgccc gccatataaa ctgggcgggc aagatgccat tggtgtcaat
     3901 ttaacgtgaa accgatgtcc cgcaaggaac tgaagttcct tgctcatagc aaaagtcatc
     3961 tgaagatgac taaatatact taaaaatctt tagtcatctt tagatgactt taactgttag
     4021 ccaagaaatt tatttcttgg cgggcgagga agccaacaga taagccattt ctagcttaag
     4081 ttgacaccaa tggcaagatg cccaccccac aagtatgact cagggcatga aaattactcg
     4141 ccttgattta aattggcact gatatcggat tcatttgttc ttctgttaat ggtgtagaca
     4201 accagcccat ttttacagga acatcatata ttcgttttgc ccccatcctc aaccagtaat
     4261 gtggcatttc tgggacaatg acctttgcca catttaagcc gatatctggg cgagttttat
     4321 ctagtactaa agtttctagt ccagcatttt tggaaatttc tacacaagtc atgacatctt
     4381 cataaatatc atcgctccaa cgctgttgat aatcttgata aacttttgct ggaactgtag
     4441 agtctggaac taaatagggc tgattttcaa tggttgcttt tttaaaccat tctcgcattt
     4501 ccacaaactc tttagttact tctacaccat cacaagtgaa agcaatttga ttaacttcgg
     4561 taactgcccg taagatggca attttagggt caaaatgcgt tccgaaaccc aggagaatcg
     4621 tttggtgttt gttatcttta agataagaga cggcaacaaa agtgggaatg tctaaatctg
     4681 cggtaatatc aattacccac aagtcacgat tgttacttct atataaatct tgtacctcca
     4741 gcagatatgg ttcgttaaaa ctagctaaat cgacagcagg acgctttaag cggttatacc
     4801 accaaatcgc tacactatcc cgttcgacta tttcaaagaa tccttgaata attgcttctt
     4861 cgagagtgtt accagtggca tcaccattag aattggcacg acaaaattca tggtcttcgg
     4921 ggagaggata gccgtagtaa caataagcag tgggaatata tttgtgggtt tgttcggtta
     4981 aagaccaaac gggagtccat tcaataactt tggtttcgtc aaagggttga ggaatccaat
     5041 caaatacccc acctctgcgg ttgaattctt cacgatattc atattgttcg gagctaaaaa
     5101 gcgaacaccg tgctggatgg atggcttttt ctcctaattc ggctaaagtg gcactaattc
     5161 ggggttcgtc accttggtag acaccagaat agcgttcaat ggcttcacaa aatccactgg
     5221 cttttgattg agagtctgtt tttcctttgc cgctactttt gtgcttgaga taacggcgca
     5281 ttcttccgat attggaagca attacaaagc tgtgaacggc attgtaggta tggttcaagc
     5341 tgtcattggg gtcagaagcc cggactaggg aagtgactac tcctgtaatt ggactaatta
     5401 agtgttgata gcgattaaca gtttggtcag gagagaaggc acgatgtccg ccatcgctgg
     5461 taaattgctt tttgcggctg ctgagaacta aaggttgaaa agcccgttcg gtcaatagat
     5521 tggggttgcc gcagctggga cattgggggc gtaaactaag aatgtgggtt tgcagatcta
     5581 agtttctctg gtcaaaggtg ataacttttc cttctaaggt aggaaagggt gttgtgtctt
     5641 ctacaccttg tttaacaatc catttagcaa tttctgtagt tgctaagtgc aaagcagttt
     5701 gtagagtaga tggaattacc gccgccggag gaggaagaca ttcaattacc tctgacttaa
     5761 ctccatttcc attgctttgt agcacagaag agttcaactc ttggctagga gaaagttgca
     5821 atgaactatt tttttgcctt aatactgaag cttccacttc tcggttaacc ctgagacgtt
     5881 gggcgagaca ttcccaacag cctgttattt gaggttggaa gattggccct aaccaaagaa
     5941 ttgtaccgac gggtttaatt agcaaccaag gttgattggc ttttagggct atttgattga
     6001 ttttatttag ttggggttgg agatagtcat cggttaaaac aactaataaa gaatgaggcg
     6061 gaaattcttg taattctcca tcccagttga tagctttgat tcccacttct tccaaagcag
     6121 tgattaacgg ttgggtggga agattgacaa ctgaagaaac ataaacttgg gtttgctgca
     6181 aacattggta agcaacttgg ggttctactt tcagcaaacc ccaaaaggca actgcttctg
     6241 gagtaagttg ggggatggct tcagttatgt agcctctggc ttgcaagcgt tctagggcat
     6301 agtcaatgtg actggggtct acttgcagtt tattgataat ttcatcaacg gtatagttgc
     6361 cgttgaggag gggaattagt tggcagtaga gttctccggt aagggcatgg gtggaagatt
     6421 cacttaataa atagacgtgc ttgggttcga taatttcagc acggaaatgg ggttttattt
     6481 ggagaatttt catattgcta tttttatgtg acaagggctg gtttatgatt tagaaattta
     6541 tagttaagtc aggtgcagaa ggaatgtttt ttttaacgca gaggggcgca aagttaaacg
     6601 cagaggtacg cagagattta cttactttga aaaggatttg ctagagagtg aattttatca
     6661 gagaagacaa acccaagcca atcctactaa gatagtaagt ttttgctaag ggtttgtttc
     6721 tctaactagt tttatgcact tccaactgat attaagacta agaaagctta ctcattcaaa
     6781 gtgttactag acgagttagt gacttaggcg agctatcagt ttattggtac taataaagta
     6841 cttgaatgaa gtgaaaaaac ttttaaactc ttaagcaagt tgatatatct gcttttgagt
     6901 taattataca ccagttgttg ggacggaggg ggatacacca ccgtctccac caccgtattg
     6961 agaagcgttt acaccgccaa ctcctacgcc accatttgtc aactcttctt ctgataagtc
     7021 gagttggata ccgggcataa cacgcactgt aggttcagaa agtttgggtt gtaagcgggt
     7081 ttgcttttga ttttctggtt tcatgagaga tttctcctaa tgaagtaaat cgaataaaaa
     7141 gtttctccaa aagaaagttt atttaacaaa attctttcac agaaactttt aatttcttat
     7201 atcacgatga acaatgctta gcaatagcct ggttttttaa gaaatatttt tgttaattgt
     7261 cttaactgtc ttaactgtct taactgtcca ctagaaattt gcttaactgt cttaactgtc
     7321 ttaactgtcc gcgagaaatt gctaaattct cttaacgatg cttaataaaa ttattttttt
     7381 tattttttag caatttagct attgattttt ggtgacaata gtcatatata atttttcaat
     7441 ctagaaaaaa tagcttgagt gcgatcgcta tttctaatcg atcgactata aagttttcta
     7501 tagaaattgc tgaagtaata agaaaatgct caaactaagt tgtttgaaat tctctagact
     7561 ctaaaacttt tttgcgacta cctcatgtct aaagtcaaga tcgtccctgg actgacacat
     7621 ctgtgggagc aaacactggg caaaccggaa atttgtgtgg cggtgctaga tgggttagtc
     7681 gatcgctctc atgtttgctt ccaaggagcc gatttgagag aaatccccgg catggtagaa
     7741 tctatcccca acccagaagg gctgatgcat cgacatggaa ctcatgttgc cagcgtgatt
     7801 tttggccagc acggctcacc agttgaagga atagcacctc gatgcaaggg tttgagtatt
     7861 cccatctttt ccgacaaagg tcgtagttta tctcaactag atctggcacg agcaattgaa
     7921 caagcagtca attccggagc gcacattatt aatctgagtg gtgggcaact ggctgactat
     7981 ggtcaggcgg atggctggtt ggaaaaagct gtggctttgt gtaaagagaa taatgtttta
     8041 ctcgtggcgg cagctggcaa taacggctgt gaatgtttgc acgttcccgc cgccctgccg
     8101 gctgtgttgg cagtgggagc aatggacgat ggcggccagc ccattgattt tagtaactgg
     8161 ggcgatacat atcaaactca aggcattctt gccccaggtg aaaatatttt gggggcgaga
     8221 gccggaggcg gagttaaaga gtggactgga acaagctttg ctacccccat tgtctcagga
     8281 gtcgcagccc tgttattgag tttgcagcag caacgtggag aaaaacccga tccgcaaaaa
     8341 gttagagacg ctattttaca aagtgccata ccttgcgaac tcaaacaaca aggcgatcgc
     8401 ccccgctgtt tagttggcaa actcaatatt ctgggcgcaa tggaaaaact taaaggagga
     8461 aaaatgtcac aacaaccaga agccttgacc gcatccggtt gtggatgcgg aggcgttagc
     8521 aagggcgatt ctttataccc cataaattat atgaatcatt cagcagtaga agatagtgga
     8581 acatcacctt cagaactagg gagttccgag tttgacggtg tgacggaatc tgcgcctggg
     8641 gaagctttag cttctgttgg tggtgcaacc tcacacccaa tttccactga agcaccatct
     8701 acatcgaatt ccaatgttgc ttctaacgtt tctttgccca cttctgccga tcttgcagct
     8761 tttcaaaacc ttctgcaatc ctacataaca acatcaaatc aacctatgtc ttatcctgtt
     8821 gcttcttctg tcaccgccag ccaagctcct agcgatatta atccggaaac tttagtttat
     8881 gctttaggta cattgggtta tgactttggt actgaagcca gacgagattc ttttaaacaa
     8941 ttgatgcctg ggattagcat tgataataca ggagtgccag caaatcccta cgatgctcgc
     9001 caaatggttg attatttggc agataacccc tcggaaggca agtctttaat ttggacgctg
     9061 aacttagaac taactccaat ttatgccatt gaacccaaag gagcttttgc tagagatgtc
     9121 tacggggttt tacaagaact gctttctggg caaattcaac cagaagatga cccagaatat
     9181 atagaacgtg ttagcatccc cggactttta accggacgca ctgttaaatt attttctggg
     9241 caatatattc cggtaattga accacaaaat acacgggggc tatatggttg gaaggttaac
     9301 accttaatta atgctgcctt agatacaatt aatgcccaag tgggagaacc acaagaagaa
     9361 aatgttcgca gaaccttggg tggcttcctc aaccggattt attatgattt ccgcaattta
     9421 ggaactactt ctcaagaccg tgccttgaat tttgctacaa ctaatgcttt ccaagcggcg
     9481 acaacttttg cccaagcagt ggcagaacaa atggaactcg ataccatcac cgttgagaga
     9541 agccccttct gtcggaagga tagtgattgt tgggatgtga aactgaagtt ctttgaccca
     9601 gaaaataacc gtcgagccaa aaaaatcttc cgctttacta ttgatgttag tgatttaatt
     9661 cctgttactt tgggcgaagt ccgtacttgg tcgagtgctt actaattatt taataatatg
     9721 caatttttga ccagtaacca aacccatgcg cctcctgata atcactggct aaagcaacgt
     9781 cagttgtata acttggcacg ttttaatcag aataaggata ttactgatag ttgggtgcga
     9841 tcgcacaatc taaatttagg caaacaagcg ggaatcaagc cagggaatta cattcttgat
     9901 gctggttgtg gggcaggaat tccggcgctg caaattgctc aggagtttcc cggaatcaga
     9961 gttgaaggca tcaccccatc ggaaatccaa gcccaagatg ctcggcagcg ggtacaggcg
    10021 tcgggattag gcgatcgcat tcagattcgg gtgggtgact ttcattacct gcccttcccc
    10081 aatggagtat ttgatgtcgc gttttttaat gagagcatta aatactccaa aaatctccct
    10141 caggcattag cagaggtcta ccgggtgctg cgtccgggag gtcacttata catcacagat
    10201 ttggtcagcc aagaactacc cttaactgag caacagcagc gagaattggc aaagttaaat
    10261 caggatttaa tttgtcatat ttattctctg agtcaattag caaaggcagg tcaacaagca
    10321 ggttttcaaa atattgagtt tggagatttc accaaacact tgcccgcagg agatatgagg
    10381 aagctgtggc tgctaaatcc tgctgccaat atgccagttt attatggaga aattaaagct
    10441 agtaaaggtg tagcttctca aatcacccca agtcaaaagc tggaagttcc cgttcaaacc
    10501 cgaactagtc gtttagttta tgcactgggt actgtaggct atgactttgg ctcccaagca
    10561 cggcgagatt cttttaaaca actaatgcct gggattgcaa ttgatggcac tgtaattcca
    10621 gccaatcctt acgacgcccg tcaggtggta gattatctag tagataaccc tccagaagca
    10681 aaatctctaa tttggacgtt gaatttagac ttaactccaa tttatgcgat cgaacccatt
    10741 gaagcttttg ctgatgatgt ttatgaaacc ttacaactgc tgttagccgg acaaatagaa
    10801 ccacaagaca gcgataatta catcgaacgg gcaagtattc cagcaaggct gacaaataga
    10861 acagtccagc tattttctgg gcaaattgtc ccggtgattg aaccagaaaa tatccggggg
    10921 atgtatggtt ggaaaattaa tagcttggtg gatgctgctt tagagacgtt agcaacagaa
    10981 actgaaacaa tcgaagccca aagagcaaga gtgcagcgta ccctaactag tttcctgaac
    11041 cgcatctact acgacctgcg aaactgggga caaactgaca gagacagagc gttgaatttt
    11101 gcggctacta atgcttttca agcagcttct acctttaaag aagcagtagc ggcaggtctg
    11161 gaactcgaca gcattgagat atataaaagc gcttactgtc ggtttgatag caactgttgg
    11221 gacgtgaaat tgaagttttt tgacccagaa agtaaccgtc gggcgagaaa aatcttccgt
    11281 ttcaccattg atgtccgcga gatcatgccc gtcaccttgg gcgaagtccg ttcttgggct
    11341 gcaccgaaat aagctagatt atttatcaag caaatattaa ctttggacaa ctatgagatt
    11401 accaaaactt gctcctccag tgaaaagacc ggatatcatt tatccgcatc gctctgtgga
    11461 tgtaattaat ggcagagtag aagatttggt tcacattcgc atggatttat tacacggagc
    11521 caactataac gatcctccag cttttaatta cagtgcttgt tctcaatctg gctatggatg
    11581 tggcggttgg tgaattcaga attcaggagt cagaattcag aattcaggag tcaggagtca
    11641 gaatttaatt ctgtggaact agcaaatgaa taaatggatt tacagcacat ctcatctaaa
    11701 ttagataaag gactgacaaa taaaaaatat ccaattctct cttgtggggt gggcgtctcg
    11761 ccattggtgt caatttaacg taaaaccgat gtccagcaag gaacttcagt tccttgctca
    11821 tagcaaaagt catctgaaga tgactaaata tacttaaaaa tctttagtca tctttagatg
    11881 actttaactg ttagccaaga aatttatttc ttggcgggcg aggaagccaa cagttatctg
    11941 aaaatgacta aatatactta aaaatcttta gtcatcttta gatgacttta actgttagcc
    12001 aagaaattta tttcttggcg ggcgaggaag ccaacagata agccatttct agcttaagtt
    12061 gacaccaatg gcaagatgcc caccccaaaa gatttatcat attagggtgt gtagtttgtt
    12121 agttgcaagg tgctgtaagt ctttcattaa atatttgagt tcaaaaaata ttttgtcggg
    12181 tgggtaatga atacccaatt ctccacacat caccagggta agaaaataaa ttatggtgac
    12241 ttataaaccg attcaaactg atagcgaacc ttacttagaa tacatcggta aacataaacg
    12301 tgcctttgac atttacgaag acctttatcc cgttaaactt tttgaagatt ttgtgcaagt
    12361 agagggtaga caaggaggta tgtttcgctg cggttgtaat gtaactaaag atgaattgtc
    12421 tccggctcgg tttgctcttt tctttcctac acaagacgat ttgcaattgc ttcacaaccc
    12481 ccaacaacag ctcgaagcag cctttgactt ctttcgcaaa gtggaaggtc gagcagaagt
    12541 caaagtcaat tatcatctga ttgaaaagtt ttttggtaag aatcctgatt ttcgagggat
    12601 ttattttatt gctgtgggtg tagatgcaca aacagaaatt gctgagtcac gcttaaagct
    12661 atttattcat ttgagtaatg cccccgacaa gatagaatcc gccattgctt tgtgtggtga
    12721 tagcccaaca ttgcgagcat tcttagtcaa caatgattta ttagtaggtt ttgattttta
    12781 tttagatggt cgcagtgaaa ttgagattta tccaactatc tatcaagaag agttggaacg
    12841 ggctgatatt caaagcagga tactaccact tttacccccg cgtgctttac cactattaaa
    12901 gcagtgtgaa gtcttgcaag tcggctttag tccggcgaat aaaagtaata ttctttattt
    12961 cgattttgtt agcgatccca atacctttat cgataatctt ggtaatgaga tggcaaaaaa
    13021 agttcatgcc tattaccgcc atcaacctgt caaacatgta ctagttggta tttctgaaaa
    13081 agacttgtat gctagctcaa ttgagaaagt gaagttgtat tacttcaaga agtaatgtat
    13141 atcaaaaaaa gaaaaaacta aataacaaaa atttaaatac aaatcaaagc tatggtgaca
    13201 tataaaccga ttccaactga tagcgaacgc tacttagaat acattcgtaa acatcaactt
    13261 gcctttgata tttacgaaga cctctatccc ctgaaacttt ttgaagattt cgtgcaagca
    13321 gagggtagaa aagaaagttg gttttacaat tgctgtaata tagataaaga tcaattatat
    13381 ccagcccgct ttacctttgg ctatcctccc caaaaagaag ctaaattgct gcacaatccc
    13441 caacggcaac tccaggaagt ctttcacttt tttcgccaag tagaaaatcg gccagaagta
    13501 aagctcaatt atcgttttat tcaccagttt tttagcattc atcctgatgt gcgtggcatt
    13561 tgctgggttg gggttggggt agatactcgc acggttatta ctgaatctcg cctcaagcta
    13621 tttattggac tcaaggatgc tcccgacaag gtagaaactg ccattactct gtgtggagat
    13681 agtcccacgt tacgagcatt tttactcaac gattacaata attacttgtt aataggattt
    13741 gatttatttt ttgacaatca cagcgaaatc gagctttatc cgactatttt tgaagataag
    13801 ttacgactat ctaatgtccg cagccaaatt acaccacttt taccccctcg tgctttaccg
    13861 ttactcgacc aatgtgaagt ttttcaaatc ggcattagtc cagccaatga cagtaatatt
    13921 ctttactttg atgatgttta cgatcccaat aattttgttg ataattttgg caatgagatg
    13981 gtcaaaaaag ttcatgccta ttaccgccat caacctatca aaaaattgct aattggcgct
    14041 cctgaaaaag acttttacgc tcaatcaatt gaacatgcga agctgtatta taggatgaat
    14101 tgatgtctat tattctaaat aagaccaagt ttgtgcaaat aatctaaaaa atcaaattag
    14161 gagactttat catcatggct aaaaagcagc aaactccaga aaactcagaa ccaactccaa
    14221 taacacctcc aataaaacaa ccagaaggaa ttcctaaaca aattgaatct catctgttgg
    14281 cgaccggttt agaagattac ggtttttggt ggcaagaaat gttaaaagca caagccaatt
    14341 ccgacaaaga gcgcaaacct ttccgccggg gtcggatttg gtcttgatta cagcagtttt
    14401 catgtatttg atccacatct attgtagggg catagcattg ctcattag
//
