LOCUS       BGC0002491             17030 bp    DNA     linear   BCT 01-APR-2015
DEFINITION  Pseudoalteromonas piscicida strain S2040 contig0074, whole genome
            shotgun sequence.
ACCESSION   BGC0002491
VERSION     BGC0002491
KEYWORDS    .
SOURCE      Pseudoalteromonas piscicida
  ORGANISM  Pseudoalteromonas piscicida
            Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
            Pseudoalteromonadaceae; Pseudoalteromonas.
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 17:49:19
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 1876
            Orig. end    :: 18906
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1653
                     /EC_number="2.7.7.58"
                     /NRPS_PKS="Domain: AMP-binding (30-442). E-value: 3.5e-73.
                     Score: 238.3. Matches aSDomain:
                     nrpspksdomains_TW75_20430_AMP-binding.1"
                     /codon_start=1
                     /gene="entE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     415.6; E-value: 3.8e-126)"
                     /gene_kind="biosynthetic-additional"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_017219309.1"
                     /locus_tag="TW75_20430"
                     /note="bifunctional 2,3-dihydroxybenzoate-AMP
                     ligase/S-dihydroxybenzoyltransferase; activates the
                     carboxylate group of 2,3-dihydroxy-benzoate forming
                     (2,3-dihydroxybenzoyl)adenylate then catalyzes the
                     acylation of holo-entB with 2,3-dihydroxy-benzoate
                     adenylate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /product="enterobactin synthase subunit E"
                     /protein_id="KJY85277.1"
                     /sec_met_domain="AMP-binding (E-value: 3.1e-84, bitscore:
                     272.4, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKSCVSWPVEDAERYRNLGYWIDEELSQILERQVQENPTQIAVI
                     DNDIEKSYACLEKEATRLASYLAAQGLCAGDTAIVQLPNCYEFYVVFFALQKLAVLPVN
                     ALMNHNQVELTHYAAALQPKLIIADTKHSLFGDSQFFEQLKACTPQLQYVLLRGQSTWA
                     KELGEKLHASACFEHFDSLPFRKHQADNVAFFQLSGGSTGTPKLIPRTHNDYYYSVRQS
                     VEVCQWNKQTRYLCALPAAHNFPLSSPGALGVFYAGGTVICAPEPSPSSCFRLIEAHKP
                     NWAALVPPAVSLWLDSRERQRYDLSSLTVLQVGGAKLSATLAARMEKELVVTLQQVFGM
                     AEGLVNYTRLDDDEWTRHHTQGRPMSAADEIRIVDENNQPVPQGEAGLLTTKGPYTFRG
                     YLNAELHNLAAFTEDGFYCSGDVVKQTASGDIIVEGRDKDQINKGGEKVAAEEVENQIL
                     AHPAVHDCAVVAMPDSLLGERICAFVVPREELDQRLTRATLNRFVRGRGLADYKYPDRF
                     EFIASLPKTKVGKIDKRALREHIANLLTSSQTA"
     subregion       1..17030
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="NRPS"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..17030
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..1653
                     /gene="entE"
                     /locus_tag="TW75_20430"
     aSDomain        91..1326
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TW75_20430_AMP-binding.1"
                     /evalue="3.50E-73"
                     /label="TW75_20430_AMP-binding.1"
                     /locus_tag="TW75_20430"
                     /protein_end="442"
                     /protein_start="30"
                     /score="238.3"
                     /tool="antismash"
                     /translation="LERQVQENPTQIAVIDNDIEKSYACLEKEATRLASYLAAQGLCAG
                     DTAIVQLPNCYEFYVVFFALQKLAVLPVNALMNHNQVELTHYAAALQPKLIIADTKHSL
                     FGDSQFFEQLKACTPQLQYVLLRGQSTWAKELGEKLHASACFEHFDSLPFRKHQADNVA
                     FFQLSGGSTGTPKLIPRTHNDYYYSVRQSVEVCQWNKQTRYLCALPAAHNFPLSSPGAL
                     GVFYAGGTVICAPEPSPSSCFRLIEAHKPNWAALVPPAVSLWLDSRERQRYDLSSLTVL
                     QVGGAKLSATLAARMEKELVVTLQQVFGMAEGLVNYTRLDDDEWTRHHTQGRPMSAADE
                     IRIVDENNQPVPQGEAGLLTTKGPYTFRGYLNAELHNLAAFTEDGFYCSGDVVKQTASG
                     DIIVEGRDKDQIN"
     gene            1673..2557
                     /locus_tag="TW75_20435"
     CDS             1673..2557
                     /NRPS_PKS="Domain: PP-binding (220-281). E-value: 2.5e-13.
                     Score: 42.3. Matches aSDomain:
                     nrpspksdomains_TW75_20435_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:isochorismatase (Score: 343.5; E-value:
                     1.1e-104)"
                     /gene_kind="biosynthetic-additional"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_017219310.1"
                     /locus_tag="TW75_20435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Isochorismatase"
                     /protein_id="KJY85278.1"
                     /sec_met_domain="PP-binding (E-value: 4.9e-14, bitscore:
                     42.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIPRISSYPLPSSRPENRTEWKINPSKAVLLVHDMQSYFVNFYD
                     TTQAPMRSLIQQISNLIVAAKAAGIPVVYTAQPANQDPKDRALLTDFWGTGLTEQDTSI
                     LPALAPTADDKVYTKWRYSAFKRSTLLEDMQAQGRDQLIICGIYAHIGILSTALDAFMY
                     DVKAFVVADAVADFGEAEHLHAMEYVSSRCGQVQLLDEVIAACTPQNTQQKLSLDSMQE
                     AIAALLCIDVEEVDPEENLLYLGLDSIRVMTLIDQWRAQGIDITLAELAESTTLNEWYE
                     ILVGKAPLAMEPA"
     aSDomain        2333..2515
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TW75_20435_PP-binding.1"
                     /evalue="2.50E-13"
                     /label="TW75_20435_PP-binding.1"
                     /locus_tag="TW75_20435"
                     /protein_end="281"
                     /protein_start="220"
                     /score="42.3"
                     /tool="antismash"
                     /translation="QEAIAALLCIDVEEVDPEENLLYLGLDSIRVMTLIDQWRAQGIDI
                     TLAELAESTTLNEWYE"
     gene            2554..6885
                     /locus_tag="TW75_20440"
     CDS             2554..6885
                     /NRPS_PKS="Domain: Condensation_Starter (1-293). E-value:
                     5.1e-72. Score: 234.6. Matches aSDomain:
                     nrpspksdomains_TW75_20440_Condensation_Starter.1"
                     /NRPS_PKS="Domain: AMP-binding (451-853). E-value: 5.5e-79.
                     Score: 257.5. Matches aSDomain:
                     nrpspksdomains_TW75_20440_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (957-1024). E-value: 3.1e-19. Score:
                     60.9. Matches aSDomain: nrpspksdomains_TW75_20440_PCP.1"
                     /NRPS_PKS="Domain: TD (1067-1299). E-value: 5.1e-63. Score:
                     204.4. Matches aSDomain: nrpspksdomains_TW75_20440_TD.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRP-metallophore: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRP-metallophore: Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     234.2; E-value: 5.6e-71)"
                     /gene_kind="biosynthetic"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_017219311.1"
                     /locus_tag="TW75_20440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="KJY85279.1"
                     /sec_met_domain="Condensation (E-value: 2.2e-39, bitscore:
                     124.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.8e-90, bitscore:
                     293.0, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.2e-12, bitscore:
                     35.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 4.8e-52, bitscore:
                     166.1, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNWPLTAAQLGIWLGQAKQPTSTRYNTAEYVELNVPINAEDWLL
                     TASRVLQSTTAINVRFIEHQGVPTTQLLSEAERVAKLGDYIDLSESDDAAFAAQQLLIK
                     WQAQSYDLANGDLYRHVLIRLATNHYYWALGAHHIAMDGFSFALVSNRVIQAYEGAQVT
                     ADETQYQAVITEDSQYRQSKWYQQDRLYWHQQLQGLDNAAALTTQVLNTEADKEKVTTQ
                     LSATLLNQLEARANSVDANWSELLLTVVSAIVYQRTGVAKTIMGMPVANRMASKAANTP
                     CMHMNIVPVPVDFVLVESFEGLIRQVSEQLRQARRHFRYRYEDLKAEAVTLQLPTRLFG
                     AVVNILPFERHETCQGEAVTSHTVSAGPVEDIAFIFIKQMDGRIRFEIEGNQSGYSKAE
                     LLSIQSETLQFLHAVAVDLQMPLRVTNRDFAISETPAPVEQSDVLSCLLELSRKSPKSI
                     AVQTIDGHAISYERLVQQVLWLAKRLRVKASVDDGTVLLALPRSQYTVIAMWAVFAAQH
                     RFVFIDSDAPSARNRSIIADAKPCLALVDDSSEGLAEIRDAGVTQLNIASELANAQLEA
                     FAATPSILPLLEQAYLIYTSGSTGKPKGVQISHDALAGFVSGARLAYQVGAQDRVLQFA
                     PFHFDTCIEEVFVTLTAGATLILRNDAMLESFEDFLAELENQRITILDLPTAYWHELCR
                     HLCATRRMLPASIHTIIIGGEAVNRQRVEAWREQFKQSVRLLNTYGPTEATVVATAIDL
                     SKATNPSLIGLPLPGRGAVVMREKGQIAKFGERGELWLTGVGLSSGYLGLAEQTAAAFV
                     SFWDPQAMRSIAAYRTGDIVRMHKSGQLEYLGRVDAQLKISGYRIEIGEIEAVLLSVPA
                     ILEAAVTVVKNAQGSATALAAHIVSTTALALNDLRTVLSEQLPAPMLPAQLYQYEALPK
                     TPANKIDRKRLSEQQNAACDAEVMSDFETQIAAVWREVLGVSNVRLADNFFSIGGQSLQ
                     CIQVAGRLTQLLNKTVNVAFLFAHPKLVDLCNALQLGDELAHKTNADIATTIKADLAQF
                     RDRLLQVQPHTLTETSEETVLLTGATGFVGAQLLAHLLAKPDLRVICSVRANNIEHGFE
                     RLQQAFDMQNLGEIDSSRITLLLGDIASSQLGLSDADYQQLGMRVTRILHNAAHTSVLR
                     DYSSLKVANTDATAELLMFAKYFSLPFNHVSTIAVAPQSGVPLDEAFIPYHSGLHDGYQ
                     QSKWVAEAMVETAIQQGVAAQVYRLARVTGAKQSGYINHNDLVWRILRSGLKFHRLPEL
                     AVSEPWTPVDRVAKFVSAQTLTPTTAAVFNVTPQTLVSLPELYQWLIDFGFVFKVESLL
                     SWLAVLEQSQDEEDLAIASFFTSQPQQSQKATTLVASNQSFISASLQHDASLQSFVKAD
                     LARYLIYAFTSNLLKIEHHPEAYKRLQTLQAKFSESKEELS"
     aSDomain        2557..3432
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TW75_20440_Condensation_Starter.
                     1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="5.10E-72"
                     /label="TW75_20440_Condensation_Starter.1"
                     /locus_tag="TW75_20440"
                     /protein_end="293"
                     /protein_start="1"
                     /score="234.6"
                     /tool="antismash"
                     /translation="SNWPLTAAQLGIWLGQAKQPTSTRYNTAEYVELNVPINAEDWLLT
                     ASRVLQSTTAINVRFIEHQGVPTTQLLSEAERVAKLGDYIDLSESDDAAFAAQQLLIKW
                     QAQSYDLANGDLYRHVLIRLATNHYYWALGAHHIAMDGFSFALVSNRVIQAYEGAQVTA
                     DETQYQAVITEDSQYRQSKWYQQDRLYWHQQLQGLDNAAALTTQVLNTEADKEKVTTQL
                     SATLLNQLEARANSVDANWSELLLTVVSAIVYQRTGVAKTIMGMPVANRMASKAANTPC
                     MHMNIVPVPVD"
     aSModule        2557..6450
                     /complete
                     /domains="nrpspksdomains_TW75_20440_Condensation_Starter.1"
                     /domains="nrpspksdomains_TW75_20440_AMP-binding.1"
                     /domains="nrpspksdomains_TW75_20440_PCP.1"
                     /domains="nrpspksdomains_TW75_20440_TD.1"
                     /final_module
                     /locus_tags="TW75_20440"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       2566..2604
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TW75_20440_0001"
                     /evalue="2.70E-03"
                     /label="C1_starter_004-017"
                     /locus_tag="TW75_20440"
                     /protein_end="17"
                     /protein_start="4"
                     /score="10.6"
                     /tool="antismash"
                     /translation="PLTAAQLGIWLGQ"
     CDS_motif       2959..3024
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TW75_20440_0002"
                     /evalue="2.30E-10"
                     /label="C3_starter_134-155"
                     /locus_tag="TW75_20440"
                     /protein_end="157"
                     /protein_start="135"
                     /score="33.2"
                     /tool="antismash"
                     /translation="AHHIAMDGFSFALVSNRVIQAY"
     CDS_motif       3343..3423
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TW75_20440_0003"
                     /evalue="4.80E-09"
                     /label="C5_starter_265-294"
                     /locus_tag="TW75_20440"
                     /protein_end="290"
                     /protein_start="263"
                     /score="28.6"
                     /tool="antismash"
                     /translation="IMGMPVANRMASKAANTPCMHMNIVPV"
     CDS_motif       3478..3588
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TW75_20440_0004"
                     /evalue="1.20E-08"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="TW75_20440"
                     /protein_end="345"
                     /protein_start="308"
                     /score="27.5"
                     /tool="antismash"
                     /translation="EQLRQARRHFRYRYEDLKAEAVTLQLPTRLFGAVVNI"
     aSDomain        3907..5112
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TW75_20440_AMP-binding.1"
                     /evalue="5.50E-79"
                     /label="TW75_20440_AMP-binding.1"
                     /locus_tag="TW75_20440"
                     /protein_end="853"
                     /protein_start="451"
                     /score="257.5"
                     /tool="antismash"
                     /translation="RKSPKSIAVQTIDGHAISYERLVQQVLWLAKRLRVKASVDDGTVL
                     LALPRSQYTVIAMWAVFAAQHRFVFIDSDAPSARNRSIIADAKPCLALVDDSSEGLAEI
                     RDAGVTQLNIASELANAQLEAFAATPSILPLLEQAYLIYTSGSTGKPKGVQISHDALAG
                     FVSGARLAYQVGAQDRVLQFAPFHFDTCIEEVFVTLTAGATLILRNDAMLESFEDFLAE
                     LENQRITILDLPTAYWHELCRHLCATRRMLPASIHTIIIGGEAVNRQRVEAWREQFKQS
                     VRLLNTYGPTEATVVATAIDLSKATNPSLIGLPLPGRGAVVMREKGQIAKFGERGELWL
                     TGVGLSSGYLGLAEQTAAAFVSFWDPQAMRSIAAYRTGDIVRMHKSGQLEYLGRVDAQL
                     KIS"
     CDS_motif       3973..3996
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TW75_20440_0005"
                     /evalue="4.00E+01"
                     /label="C1_starter_004-017"
                     /locus_tag="TW75_20440"
                     /protein_end="481"
                     /protein_start="473"
                     /score="-1.5"
                     /tool="antismash"
                     /translation="VQQVLWLA"
     CDS_motif       4321..4380
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TW75_20440_0006"
                     /evalue="2.50E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="TW75_20440"
                     /protein_end="609"
                     /protein_start="589"
                     /score="32.2"
                     /tool="antismash"
                     /translation="AYLIYTSGSTGKPKGVQISH"
     CDS_motif       4903..4986
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TW75_20440_0007"
                     /evalue="4.60E-09"
                     /label="NRPS-A_a6"
                     /locus_tag="TW75_20440"
                     /protein_end="811"
                     /protein_start="783"
                     /score="28.6"
                     /tool="antismash"
                     /translation="GERGELWLTGVGLSSGYLGLAEQTAAAF"
     CDS_motif       5077..5142
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TW75_20440_0008"
                     /evalue="2.90E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="TW75_20440"
                     /protein_end="863"
                     /protein_start="841"
                     /score="29.1"
                     /tool="antismash"
                     /translation="YLGRVDAQLKISGYRIEIGEIE"
     aSDomain        5425..5625
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TW75_20440_PCP.1"
                     /evalue="3.10E-19"
                     /label="TW75_20440_PCP.1"
                     /locus_tag="TW75_20440"
                     /protein_end="1024"
                     /protein_start="957"
                     /score="60.9"
                     /tool="antismash"
                     /translation="ETQIAAVWREVLGVSNVRLADNFFSIGGQSLQCIQVAGRLTQLLN
                     KTVNVAFLFAHPKLVDLCNALQ"
     aSDomain        5755..6450
                     /aSDomain="TD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TW75_20440_TD.1"
                     /evalue="5.10E-63"
                     /label="TW75_20440_TD.1"
                     /locus_tag="TW75_20440"
                     /protein_end="1299"
                     /protein_start="1067"
                     /score="204.4"
                     /tool="antismash"
                     /translation="VLLTGATGFVGAQLLAHLLAKPDLRVICSVRANNIEHGFERLQQA
                     FDMQNLGEIDSSRITLLLGDIASSQLGLSDADYQQLGMRVTRILHNAAHTSVLRDYSSL
                     KVANTDATAELLMFAKYFSLPFNHVSTIAVAPQSGVPLDEAFIPYHSGLHDGYQQSKWV
                     AEAMVETAIQQGVAAQVYRLARVTGAKQSGYINHNDLVWRILRSGLKFHRLPELAVSEP
                     WTPVDRVAKF"
     gene            6882..7703
                     /locus_tag="TW75_20445"
     CDS             6882..7703
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1200:vibriobactin
                     utilization protein ViuB (Score: 256.8; E-value: 3.2e-78)"
                     /gene_kind="other"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_017219312.1"
                     /locus_tag="TW75_20445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Vibriobactin utilization protein ViuB"
                     /protein_id="KJY85280.1"
                     /transl_table=11
                     /translation="MKQRQPVPPSGPRRVFCVAKRYITPHLLRITVSGEALHGFPSGYD
                     GAHIKLFFANRTTGTLSLPTRDDSGKIVWPTDRPVTRAYTVRAYRPDSNELDIDFVVHG
                     SHSPASGWAVNADVGAELGVAGPGGPDPLLADADWHVLAGDLTAVPAISALLEGFEAER
                     RAEVFIEIDSLDDKHTITAPSGVNIHWLLRQSGDCEQLARAIANVAIPSGVTSISAFIA
                     GENGAVLACRKQLIQDFGLSKKQLYAIPYWRRGQDEETYHGRRHEIMDEVY"
     gene            7704..8477
                     /locus_tag="TW75_20450"
     CDS             7704..8477
                     /NRPS_PKS="Domain: PKS_KR (11-165). E-value: 3e-10. Score:
                     32.1. Matches aSDomain: nrpspksdomains_TW75_20450_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRP-metallophore: EntA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     217.1; E-value: 4.4e-66)"
                     /gene_kind="biosynthetic"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_017219313.1"
                     /locus_tag="TW75_20450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2,3-dihydroxybenzoate-2,3-dehydrogenase"
                     /protein_id="KJY85281.1"
                     /sec_met_domain="EntA (E-value: 1.2e-110, bitscore: 358.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNECEFTGRIALVTGAYQGIGKALVEQLLARGAIVVAADIAIAA
                     SQLQEVTANHFQIHLDVTNKNLVQTVVEEIEIQLGALEYVASVAGILHMGTLLEQSDAQ
                     WLDTFAVNCHGPFYLCQAVANKMRIRKRGAIVAVSSNAASTPRVNMGSYCASKAALSAM
                     IKTLALEVAPFNIRCNLVAPGSTDTQMQQQLWRDENGAEQTIQGDLTQFKLGIPLGRIA
                     SATDIANSILFLLSDQARHITMANLLVDGGATLGH"
     aSDomain        7737..8198
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TW75_20450_PKS_KR.1"
                     /evalue="3.00E-10"
                     /label="TW75_20450_PKS_KR.1"
                     /locus_tag="TW75_20450"
                     /protein_end="165"
                     /protein_start="11"
                     /score="32.1"
                     /tool="antismash"
                     /translation="ALVTGAYQGIGKALVEQLLARGAIVVAADIAIAASQLQEVTANHF
                     QIHLDVTNKNLVQTVVEEIEIQLGALEYVASVAGILHMGTLLEQSDAQWLDTFAVNCHG
                     PFYLCQAVANKMRIRKRGAIVAVSSNAASTPRVNMGSYCASKAALSAMIK"
     CDS_motif       7740..7805
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TW75_20450_0001"
                     /evalue="1.20E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="TW75_20450"
                     /protein_end="34"
                     /protein_start="12"
                     /score="11.5"
                     /tool="antismash"
                     /translation="LVTGAYQGIGKALVEQLLARGA"
     gene            8477..9745
                     /locus_tag="TW75_20455"
     CDS             8477..9745
                     /NRPS_PKS="Domain: Aminotran_3 (50-335). E-value: 3.7e-31.
                     Score: 100.2. Matches aSDomain:
                     nrpspksdomains_TW75_20455_Aminotran_3.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 249.8;
                     E-value: 7.4e-76)"
                     /gene_kind="biosynthetic-additional"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_017219314.1"
                     /locus_tag="TW75_20455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glutamate-1-semialdehyde aminotransferase"
                     /protein_id="KJY85282.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.4e-54, bitscore:
                     172.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFEEYMNNQTLSLTQSQQLQTEGEQVIAGFTQSMMKKPEQFAPGA
                     FPVYLSKGEGAIVTDVDGNQYIDFICGLAANTLGHNHPVVVNTITEHLHNGIIHSLPTP
                     IEVQTAKTLIEVIPNAEQVRFFKTGADANSAAVRLARHVTGRDEIMTVGYNGWHDQYMF
                     DTPGVPAVIAGLTHRMPLFTPADEPKIIEKIIARKDELAVVLLSVPYNRVLERPFLEQL
                     RNTCSEHGVILVFDEVVTGFRLAPGGAQEYFGIQADLVTLSKGIAAGMPLSAVAGKREL
                     MSRINELQVSTTFGGEMLSLAVCDAVLKEYQRTEFTQHIARLGAILKAEVNQVSLQLGV
                     PLKVVGYDPIPMFLFAKDPQEHVKFAVPFLAEMAKRGVLMRREVNFICGAHTEAHINET
                     IAAVRASLIAMKEAGLFDSQDAS"
     aSDomain        8627..9481
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TW75_20455_Aminotran_3.1"
                     /evalue="3.70E-31"
                     /label="TW75_20455_Aminotran_3.1"
                     /locus_tag="TW75_20455"
                     /protein_end="335"
                     /protein_start="50"
                     /score="100.2"
                     /tool="antismash"
                     /translation="SKGEGAIVTDVDGNQYIDFICGLAANTLGHNHPVVVNTITEHLHN
                     GIIHSLPTPIEVQTAKTLIEVIPNAEQVRFFKTGADANSAAVRLARHVTGRDEIMTVGY
                     NGWHDQYMFDTPGVPAVIAGLTHRMPLFTPADEPKIIEKIIARKDELAVVLLSVPYNRV
                     LERPFLEQLRNTCSEHGVILVFDEVVTGFRLAPGGAQEYFGIQADLVTLSKGIAAGMPL
                     SAVAGKRELMSRINELQVSTTFGGEMLSLAVCDAVLKEYQRTEFTQHIARLGAILKAEV
                     NQVS"
     gene            9745..11277
                     /locus_tag="TW75_20460"
     CDS             9745..11277
                     /codon_start=1
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_017219315.1"
                     /locus_tag="TW75_20460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytosine deaminase"
                     /protein_id="KJY85283.1"
                     /transl_table=11
                     /translation="MVCSYLSTQRFAALAGASGNVAFGSAQVGELESLKAVCNKPQNNA
                     LRTLIDNVFIYTADTRDTVYPKGWLLVHGKHIEALGEDGEQPSNFDQRIDGQGKLMLPG
                     LINPHWHESFVAPNAESPDDTHLEATAYSNGGNIQALGSMFGFISTVGDKLTLEEGLAI
                     ARWSMWTQLRSGTTALGDVGSANKADAMALAAIDLGMRLRVSRWGSDIMLEAHRSTPTI
                     IADTELQTADWLSLIETWDNHSSGLVGAMPSVMGAFGSSDKQLEALASIAQQYQVPYAT
                     HLAPLKNERAEVERVFGRSPIARFDAMGLLTDKLLAVHTAYASEDEYQRLVETGVNICH
                     SPAHYGMLGEATISETGQIGRFLKDGVWVSSSTDGDISFIGGMCEAMRGAHLGHNEAQN
                     CNTVCPPTLALKTASLYAAKALGWEQQIGSIEVGKQADLVLVNNDDYRYKLSGHPLRTF
                     IVTGSSLDVDSVMVAGDLVVKAGRSTRFDEHELFEDYLKAVASARKRIGGPA"
     gene            11274..12449
                     /locus_tag="TW75_20465"
     CDS             11274..12449
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 33.9; E-value:
                     1.9e-10)"
                     /gene_kind="transport"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_017219316.1"
                     /locus_tag="TW75_20465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diguanylate cyclase"
                     /protein_id="KJY85284.1"
                     /transl_table=11
                     /translation="MNQTRLATWFLLLASTMTVLASATLAPALPAMTVAFSGIEHAAFW
                     TKMTLALPGLVIALCAPIAGSLVDKWNSQKLLVNALILFVLSGGLGYYWQDSLWLILGS
                     RAMMGVAVAFIMVSGTTIAGRYFEGPKFSQFMGLQAAFGGFGGVLFLALAGFLAEQHWT
                     WVFAIYSLALLVLPGVWLWVKVPPVSLPPQTQSLPSSWMNRTFLLCCTLALVEIIVLYG
                     LTIHLPFYLSAQQASASTIGLVIAGFLFAMSCVSMGYGRVRQYLNIKQAHLVGWLIVAT
                     GFSLLGFVNTFSSIVCASFVIGAGLGLIRPNLVVWLFEFVPPPMRGKAMGIMTTCYFTG
                     QFISPVLFEPMIASLGFQLFFIYLAMTIASVAVLVAGIYLLNQKTWLKQLD"
     gene            complement(12455..13429)
                     /locus_tag="TW75_20470"
     CDS             complement(12455..13429)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1260:AraC family
                     transcriptional regulator (Score: 57.9; E-value: 2.1e-17)"
                     /gene_kind="regulatory"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_017219317.1"
                     /locus_tag="TW75_20470"
                     /note="transcriptional regulator, VCA0231-like protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="transcriptional regulator"
                     /protein_id="KJY85285.1"
                     /transl_table=11
                     /translation="MEGNISNAELNNKLLLASGLNCRINGFDIEQLIAIGRLSFLPLSD
                     DLQLHTCQCIEQQQTSSCAELQPSVNITVLLKGVVEFRVANKYYRFSAENAPQAFVNLI
                     TQPEPFTRYLQKHQEVEKVTLSVSPRWLVARLSQQYQSLFTTTKVIELPLQAKAISLSQ
                     QLLQTPKQPSLAERLECEGVVMQWLSCFLPHLIDSSNQSHVMYKQPFHSDIEVKKNKII
                     DLLSEGKSVEQVALELAMSLSSLQRFFKRSFNTTPKQYVKQSKLNKARHALLVEGLSIG
                     EAAYLAHYDHVANFIAAFKKQFGVTPMQFVKAHRSDTLGATSG"
     gene            13563..15707
                     /locus_tag="TW75_20475"
     CDS             13563..15707
                     /codon_start=1
                     /gene_functions="transport (smcogs)
                     SMCOG1082:TonB-dependent siderophore receptor family
                     (Score: 253.4; E-value: 1.2e-76)"
                     /gene_kind="transport"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_017219318.1"
                     /locus_tag="TW75_20475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Fe-regulated protein B"
                     /protein_id="KJY85286.1"
                     /transl_table=11
                     /translation="MLVSRTKQLGFRPSTLALALAGVLSAPALADSGDAGKIERIEVWS
                     TAVKTSALYLKEQEIADKQADHISDLLRSIPGIDVGGAHSLNQRITIRSMDDKDLKISI
                     DGAAQNTYMYHHMGNLQIHADILKSVDIETGTNSVINGGLGGSVRFETKEARELLSGDD
                     RFAVRVSAGAADNAGHNYSVTGFGLLTDDVDFLAYYNHVERDNYEVGGGKILDQNDEVV
                     AGTDGEVKGLEGEVSDALIKFGWNINNNQRLALSFETYKDEGDYSYRPDMGLATDVAIT
                     NSLQIPLLWPTEFTRDTITLSYDLNWGENSSLKASLYSNTSELYRDESGWSMSPSPRFQ
                     AYAGEVTGEAENTGLNLLGETLVTGLFAEEEHTFTYGADYVKHDTQYDLAIASGGSKHS
                     EESAKNLAFFIQDKIDFGNGLAVIPGVRHDSYDIDSGTVNNDYSEFSLSLAAEYQFNEN
                     LMVKLSSTELFKGPEISEVFVGAGLGDNRNQDIEAETGVNTEFSIAYDGEVGTNKTLRL
                     GATIFRTQIDDYIFDNAALPGGAPRETWKDNVGDMTVDGFEAYAGFALDAFSFQLTYSN
                     SESELDAFTQYASLDGARLEREQGDTISADFTYQITAYNLTLNWDFMVVDDVAQGTDLY
                     GADLDNSKDGFTVHNISAHWQPESVPSLHVRLGVDNLFDEFYSSQSSKNGVSFHPVFGE
                     LFLMDYEPGRNIKASVSYQF"
     gene            complement(15810..17030)
                     /locus_tag="TW75_20480"
     CDS             complement(15810..17030)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRP-metallophore: EntC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018:isochorismate synthase (Score: 322.2; E-value:
                     1.2e-97)"
                     /gene_kind="biosynthetic"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_017219319.1"
                     /locus_tag="TW75_20480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isochorismate synthase"
                     /protein_id="KJY85287.1"
                     /sec_met_domain="EntC (E-value: 7.6e-139, bitscore: 452.8,
                     seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSEISALQSTNTTLESIDLSQQAFFVSGTRCLVTQRPHHRFTTQ
                     IKDHDVLVSQLKRELAKCSSNDAIAFGVLPFCKTQQAQFIVTEQAHQYDKETFKQWLNA
                     KGTLKQDTMPSLERISHRQSQHHYESAIREAKKLFAKEQLEKIVLGKQVDLYFDAPLSP
                     ETILTNLLTQSPSGFHFSFPTEQNSTLMGVSPELLLRKHGQAVLSNPLAGSAKRSNDKA
                     TEFKLKQALMASKKDRYEHAVVLVEMSQVLQPWCQKLTIPSSPSLLSTATMWHLSTEVE
                     GQLADANTHVLALANRLHPTPALCGKPTADAYPWIHSLEGESRDFFSGIVGWCDKHGNG
                     EWVVVIRSGEISANHARLFAGAGIVAASDPTAEWLETEAKLSTMLNALESNQTYHQLSQ
                     IKMRATA"
ORIGIN
        1 atgttaaaaa gttgtgttag ttggcctgtt gaggatgcag agcgatatcg caatcttgga
       61 tattggatag atgaagagtt aagtcagatc ttagagcgtc aagtacaaga aaatccgact
      121 caaattgccg ttatcgataa cgatatcgag aagtcctatg catgcttgga aaaagaggcg
      181 acgagactgg cgagttattt ggctgcgcaa ggactttgtg ccggtgacac cgcgattgtg
      241 caattgccta attgctacga attctatgtg gtattttttg ctttacaaaa gctggcagtg
      301 ttacctgtta atgctttgat gaatcataac caagtcgagt taacacatta cgcagctgct
      361 ctgcagccaa agttgatcat tgcggacaca aagcattctc tgtttggtga tagtcaattc
      421 tttgaacagt taaaagcgtg tacaccacag ctacagtatg tgctgctgcg tggtcaaagt
      481 acttgggcga aagagcttgg tgaaaagctg catgcttctg catgctttga gcattttgat
      541 tctctgcctt ttcgtaagca tcaagcggac aatgtggcat tctttcaatt gtcgggggga
      601 agcacaggta cacctaagtt gatccctcgt acccacaatg attattacta tagtgttcgc
      661 caaagtgtcg aagtgtgtca atggaataaa caaacgcgat acctttgcgc tctacctgcc
      721 gcacacaatt ttccgcttag ttcacccggc gcattgggag tgttttacgc tggtggtaca
      781 gtgatctgtg ctccagaacc ctcaccaagc agctgtttta ggctgatcga ggcccataaa
      841 ccaaattggg ccgctttggt tccccctgcg gtttcgttgt ggctcgattc ccgagagcga
      901 caacgctatg acctaagctc gttaactgtg ctccaagtcg gtggagctaa gctcagtgcc
      961 accctcgctg ccagaatgga aaaagagctt gttgtgacgt tgcagcaggt atttggcatg
     1021 gcggaagggc ttgtcaacta tacccgtctc gatgatgacg aatggacgcg acatcataca
     1081 caaggtcgac caatgagtgc agctgacgaa atccgtatcg tcgacgagaa taatcaacca
     1141 gtacctcaag gagaggctgg gttattaacg actaaaggac cttatacatt tcgtggttat
     1201 ttaaacgctg aacttcataa cttagctgca tttactgaag atggttttta ctgtagtggt
     1261 gatgtggtta aacaaactgc atcgggcgac ataattgttg agggccgcga taaggatcaa
     1321 atcaataaag gtggcgaaaa ggtcgcggca gaagaagtcg agaatcaaat attggcacat
     1381 cctgcggttc atgattgtgc ggttgttgca atgccagata gtctgttggg tgagcgtata
     1441 tgcgcatttg tcgtgccgcg agaagagctt gaccaacgac tcacaagggc aacgctcaac
     1501 cgttttgttc gaggtcgtgg gctcgctgat tacaagtatc ctgaccgctt tgaatttatt
     1561 gcgtcactac caaaaacgaa agttggaaag atcgataaac gcgcgttgcg tgagcatatc
     1621 gctaatctac ttacctcatc acaaactgca taatactcaa taggataaac tcatggcaat
     1681 cccacgtatt tcttcatatc cattaccatc aagccgcccg gaaaatcgca ctgagtggaa
     1741 aattaacccg agcaaagcgg tgctattggt tcacgatatg caatcttatt ttgtgaattt
     1801 ctatgataca acgcaagcac cgatgcggtc tttgatccaa caaattagta atttaatcgt
     1861 agcagccaaa gctgctggta ttcctgttgt gtatactgca cagcccgcaa atcaagatcc
     1921 gaaagataga gcgctgctaa ccgatttttg gggaactggt ctgactgagc aagacacgag
     1981 tattttacct gcacttgctc caactgccga tgataaggtt tataccaagt ggcgttacag
     2041 cgcatttaaa cgtagtaccc tacttgagga tatgcaggca caaggtcgag accagcttat
     2101 tatctgtgga atttatgccc atatcggtat cttatctacg gcattagacg cctttatgta
     2161 tgacgtaaaa gcattcgttg ttgcggatgc tgtcgcagac tttggtgaag cagaacatct
     2221 ccatgcgatg gagtatgttt cttctcgttg tgggcaggtg caattgttgg acgaggttat
     2281 cgctgcgtgc acgccacaaa atacacaaca gaagttatct ttggactcga tgcaggaggc
     2341 gattgcagca ttgctttgca ttgatgtgga agaggttgat cctgaagaaa atttactcta
     2401 tctaggatta gattccattc gcgtaatgac attgattgat cagtggcggg cgcaaggcat
     2461 cgatattacg ctagctgaac ttgcagaaag caccacactt aatgagtggt atgaaattct
     2521 agtgggcaaa gcaccgctgg cgatggaacc tgcatgagta attggccttt aaccgctgcg
     2581 caacttggga tttggcttgg gcaggcaaaa cagcctacta gtacgcgcta taacactgct
     2641 gagtatgttg agttaaacgt gccaataaat gctgaagatt ggctgttaac tgcttctaga
     2701 gtgctacaaa gtacgacggc aatcaatgta cgctttattg aacatcaagg cgtgccgaca
     2761 actcaattac tcagcgaagc tgagcgagtg gcaaagttgg gcgactatat cgacttgtca
     2821 gaaagcgatg atgcagcgtt tgcagcacag cagcttttga taaagtggca agctcagtct
     2881 tatgacttag ccaatggcga cttatatcgt catgtgttaa tccgacttgc cactaaccac
     2941 tactattggg cacttggtgc gcatcatatt gcgatggatg gctttagttt tgcgctggtt
     3001 tctaatcgag tgattcaggc ctatgagggg gctcaagtta cggctgatga aactcaatat
     3061 caagctgtca tcactgaaga tagtcagtac cgtcaaagca agtggtatca gcaggatagg
     3121 ctgtattggc accagcagct gcaaggtctg gataatgctg cagccctgac aacacaagtg
     3181 ttaaatacag aggcggacaa ggaaaaggtt acgacgcaat tatcagcaac tttgctcaat
     3241 cagttggagg ctcgagccaa ttcggtcgac gccaactggt ctgaactatt actgactgtg
     3301 gttagcgcta ttgtctatca acgcactggg gtggcgaaaa cgatcatggg catgcctgtt
     3361 gccaatcgta tggcatcaaa agctgcgaat acgccttgca tgcatatgaa tattgtaccg
     3421 gttccagtgg actttgtgct tgttgagagt tttgaaggtt taatccgtca ggtcagtgag
     3481 caattgcgtc aagctagaag gcattttcga tatcgttatg aagatttaaa agctgaagct
     3541 gtgaccttgc aattgccaac aagattgttt ggtgcagttg ttaatatttt accttttgaa
     3601 cgtcatgaaa cctgccaggg tgaagcggtg acaagtcata ctgtgtctgc tgggcctgtt
     3661 gaggacattg catttatttt tatcaagcaa atggacggca ggattcgttt cgagattgaa
     3721 ggtaaccaaa gtggctatag taaagctgag ttgttgagta tacaaagtga aacgttgcag
     3781 tttctccatg cagttgctgt ggacttgcaa atgccattac gcgttaccaa tcgggacttt
     3841 gcgataagcg agactccagc acctgtggag cagagtgacg tacttagttg tttgttggag
     3901 ttatcaagga agtcgcctaa atcaatagcg gttcagacga tagatggaca tgctattagc
     3961 tatgagcggt tagtacagca agtgctatgg ctagcaaaac gccttagagt caaagcttcc
     4021 gtcgatgatg gtaccgtatt actagcactg cctcgtagcc aatatacagt gattgcaatg
     4081 tgggccgtat ttgctgcgca gcaccgcttc gtttttattg atagcgatgc tccttctgcg
     4141 cgtaatcgta gtattatcgc tgatgctaag ccatgcctag cattggtgga tgatagtagt
     4201 gaagggttag ctgagatccg cgacgctggc gttacacagc ttaatattgc aagcgaactt
     4261 gcaaatgctc agcttgaagc gtttgctgcc accccatcaa tacttccttt attagaacaa
     4321 gcctatttaa tctatacctc aggttccacg ggtaagccta aaggggtgca gataagccac
     4381 gacgcattgg caggttttgt aagtggcgca cgtctggcct accaagtagg tgctcaagat
     4441 agagtattac agtttgcgcc ctttcatttt gatacctgta ttgaagaagt ctttgttact
     4501 ttgacggccg gtgcgacgtt gatactgcgt aatgacgcca tgttggagtc ttttgaggat
     4561 tttcttgccg aactagaaaa ccaacgtatt actatactcg atttacctac cgcttactgg
     4621 catgagcttt gtcgacacct gtgtgcgact cgccgtatgc ttcctgcgtc aattcatacc
     4681 atcattatag gcggagaggc ggttaatcgt cagcgtgtag aagcgtggcg tgagcagttt
     4741 aaacagtctg tacgtttact taacacttac ggcccgacgg aagctacagt cgttgctact
     4801 gccattgatc tcagtaaagc aacaaatcca agcttaattg gtttgccctt acccggacgc
     4861 ggtgctgtgg taatgcgtga aaaaggccag atcgcaaaat ttggcgagcg cggagagctc
     4921 tggcttacgg gcgtgggttt atcgagcggt tatttgggtc tggccgagca aactgctgct
     4981 gcgtttgtct cattttggga tccgcaagct atgcggagca tagcggctta ccgtaccgga
     5041 gacattgtgc ggatgcataa aagtgggcaa cttgaatatc ttggccgcgt cgatgcccag
     5101 ttaaaaatca gtggctaccg gattgaaatt ggtgagattg aagctgtgct attgagtgtt
     5161 cctgccattc ttgaagccgc ggtgactgtg gttaagaatg cgcaaggtag tgcaactgct
     5221 ttggcggcgc atatagtgag taccaccgca ttggcgttaa atgatttacg cacagtgctg
     5281 agtgagcagt tacccgctcc aatgttacct gcacagcttt atcaatatga agctttacct
     5341 aaaacaccgg ccaataaaat cgatcgcaag cgattatctg agcagcaaaa tgccgcctgt
     5401 gacgctgagg ttatgagtga ctttgaaaca cagatagctg ccgtttggcg ggaagttctg
     5461 ggcgtgagta atgtccggtt agccgataac tttttcagca ttggggggca gtcactccaa
     5521 tgtattcaag ttgcagggcg gctaacacaa ctacttaata aaacggtgaa tgtcgcattt
     5581 ttatttgcgc atcctaaact cgtcgacttg tgtaatgcac tacagcttgg tgatgaatta
     5641 gcacacaaga cgaacgcgga tatcgcgaca acgataaaag cagaccttgc tcaatttaga
     5701 gataggctgt tgcaggttca accgcataca ctaactgaaa ctagtgagga aacggtgttg
     5761 ctgaccggtg cgacgggatt tgttggtgcg cagttactgg cgcatttact tgcgaagccg
     5821 gatctaagag taatttgcag tgtaagagct aataacatag agcatgggtt cgaacggtta
     5881 cagcaagcct tcgacatgca aaaccttggt gaaatagata gttctaggat aacgctgttg
     5941 cttggagata ttgcctcgtc acaacttggg ttgagtgatg cagactatca acagctgggc
     6001 atgcgcgtga ctcgaatatt acacaatgca gcgcatacct ctgtgttacg tgattacagt
     6061 agcttaaaag tggcaaatac agatgcgaca gcggaattat tgatgttcgc gaaatatttt
     6121 agtttgccat ttaatcacgt ctcgactatt gcggtcgcac cacagtccgg agtgcctctt
     6181 gatgaggcgt ttattcctta tcatagtggc cttcacgatg gttatcagca atcaaaatgg
     6241 gttgcggaag cgatggtaga aaccgctatt caacaggggg tagcagcaca agtatatcgc
     6301 ttagcacgag ttacgggggc caaacaaagt ggctatatta accacaatga cttggtatgg
     6361 cgcatcttgc gctctggact taagtttcat cgtttgcctg agcttgctgt gtctgagccg
     6421 tggacgcctg tagatagagt tgccaaattt gttagcgcac aaactttaac gcctactaca
     6481 gcggctgtct ttaacgtgac accgcaaact ttagtgtcgt taccggaatt gtatcaatgg
     6541 cttatcgatt ttggttttgt tttcaaagtg gaatctttgt tgagctggtt ggctgttctt
     6601 gaacagtcac aagatgaaga agacctagcg attgcaagtt tctttacaag tcagccgcag
     6661 caatcacaaa aagcgacgac attggttgcg agcaatcaga gttttatctc agcaagtctc
     6721 caacacgatg cgagtttgca gtcttttgta aaggccgact tagcgcgcta cctcatttat
     6781 gcctttacca gcaacttgct aaaaattgaa caccatcctg aagcgtataa acgactgcag
     6841 acattacagg ctaaattttc tgaatcgaaa gaggagttgt catgaagcag cgacaaccag
     6901 ttccacctag cggtcctcgc cgtgtatttt gtgtagccaa gcgctatatc acgccacact
     6961 tgttgcggat caccgtttct ggcgaagcgt tacatggctt cccaagcggt tatgatggtg
     7021 cgcatatcaa gctattcttc gcaaatagaa caaccggcac attatcactc ccaacacgtg
     7081 atgatagcgg taaaattgtt tggccgacgg atcgcccggt gacaagggct tacacggtgc
     7141 gagcttatcg accagacagc aatgaattgg atattgattt tgtagtgcac ggctcccaca
     7201 gtccagcatc tggctgggcg gtcaatgccg acgttggcgc tgaacttggg gttgcagggc
     7261 ctggtgggcc agatccattg ctagcggatg cggattggca cgtgcttgca ggcgatctta
     7321 cagctgttcc ggcgattagc gcgctgctcg aggggtttga ggcagagcga cgagctgaag
     7381 tctttattga aatagattca ttagatgata agcatactat tactgcacca tcaggtgtca
     7441 atattcattg gttgctgcgt caaagtggtg actgtgaaca acttgcaaga gcaattgcaa
     7501 atgttgcaat accctcaggt gtgacttcta tcagtgcttt tattgctggc gaaaatggtg
     7561 cagtactggc gtgtcgtaag cagcttattc aagattttgg gttatcaaaa aaacaactgt
     7621 atgccattcc atattggcgt cgcggtcagg atgaagaaac ctatcatgga cgccgccatg
     7681 aaatcatgga tgaggtgtac tagatgtcca atgaatgtga atttacaggg cgtattgcgt
     7741 tggtgacggg agcttatcag ggcataggta aagcactggt cgagcaacta ttagcacgcg
     7801 gagcgattgt agttgcggca gatattgcta ttgccgcgag tcaactccaa gaagtaacgg
     7861 ctaatcactt ccaaatccat cttgatgtta ctaacaaaaa ccttgtgcaa accgtagttg
     7921 aggaaattga aatccagctc ggtgcactag agtatgttgc cagtgtcgct ggtattttgc
     7981 acatgggaac gctgctagaa caaagcgatg cccagtggct tgatacgttt gccgtaaatt
     8041 gccatgggcc attttattta tgtcaggcgg tggcgaataa gatgcggata agaaagcgtg
     8101 gtgccatcgt tgctgtgagc agtaatgcag ctagcacacc tagagtgaat atgggaagtt
     8161 attgcgcttc aaaagcggcc ttgagtgcga tgattaaaac gctagcccta gaagtcgcac
     8221 cgtttaacat tcggtgtaac ctagttgcgc cggggtctac cgacactcaa atgcagcagc
     8281 agctatggcg cgatgaaaat ggtgctgagc aaaccattca aggcgattta acgcaattca
     8341 aattaggtat tccactaggt cgtatcgctt cagcgacgga tatcgcaaac agtattttgt
     8401 ttttactcag tgaccaagct cgacacatca ccatggctaa tctgttggtt gatggtgggg
     8461 cgacactagg acattaattt tcgaggaata tatgaataat caaacacttt ctttgacgca
     8521 atctcagcaa cttcaaaccg aaggtgaaca agtgatcgcg ggttttactc aatcaatgat
     8581 gaaaaagcca gagcaatttg caccaggggc tttcccggtt tatctgagca agggagaggg
     8641 agccattgtc accgatgttg atggtaatca atatatcgac tttatttgtg gtttagcggc
     8701 caatacttta ggtcacaatc accctgttgt tgtcaataca attacagagc acttgcacaa
     8761 cggcattatt cattcgttgc ctacacctat cgaagtgcag acggcgaaaa ccttaattga
     8821 agtgatccca aatgccgaac aagttcgctt ttttaaaacg ggtgcagatg ctaactctgc
     8881 agcggtaagg ctcgcccgtc acgtgacagg tcgggatgaa attatgacgg taggttataa
     8941 cggttggcat gatcagtaca tgtttgacac accaggggta ccagctgtta ttgcagggtt
     9001 aactcatcgc atgccattat ttactcctgc agatgaacca aaaatcattg aaaagatcat
     9061 tgcaagaaaa gatgaacttg cggtggtgtt gctatcggtt ccatataatc gcgtcttaga
     9121 aagaccattc ctagaacagt tacgaaatac ttgcagtgaa cacggtgtga tcttggtgtt
     9181 tgatgaagtg gtaacgggct ttagattagc acccggcggt gcgcaagaat acttcggtat
     9241 tcaggctgat ttagtgacct tgtctaaggg gattgcggcg gggatgcctt tatctgctgt
     9301 ggctggcaag cgtgagttga tgtcgcggat caatgagctg caagtatcga ctacttttgg
     9361 tggcgaaatg ctctcgctag cagtgtgtga tgctgtatta aaggaatatc aacgcactga
     9421 attcactcag catatagcaa gacttggcgc aattttaaaa gcagaggtta atcaggtttc
     9481 actgcagtta ggtgtgccac tcaaagtagt tggttatgac cctattccga tgttcttatt
     9541 tgccaaagat ccgcaagagc acgtaaagtt tgcagtacct tttctagctg aaatggcaaa
     9601 acgtggtgtg cttatgcgcc gtgaagtgaa ttttatttgt ggcgcacata ctgaagcgca
     9661 tatcaacgaa acaatagccg cagttagggc atcactaata gcgatgaaag aagcaggcct
     9721 ttttgattct caggatgcca gctaatggtt tgttcttact tgtcgacaca gcgctttgca
     9781 gcacttgctg gcgcttcagg aaatgttgcg tttggtagtg ctcaggttgg tgaacttgag
     9841 agcttaaaag cggtttgtaa caagccgcag aacaatgcac tgcgcacgct aattgataat
     9901 gtttttatct acacagcaga cacaagggat actgtttacc caaaaggttg gttacttgtt
     9961 cacggcaaac atattgaagc gctgggtgag gatggagaac aacctagtaa cttcgatcaa
    10021 cggattgatg gtcaaggtaa gttaatgctg ccaggactta tcaatcctca ttggcatgaa
    10081 agttttgtcg cgccaaatgc tgagtcacct gatgacaccc acttagaggc cactgcgtat
    10141 tcaaatggtg gcaatattca agcactaggt tccatgtttg gctttatttc caccgtggga
    10201 gataagttga cgctggagga aggtctcgcc atcgcccgtt ggagtatgtg gacgcaattg
    10261 cgttctggta caactgcgct gggagatgtt ggttcggcaa ataaagctga cgcaatggcg
    10321 cttgctgcga ttgatctagg tatgcggtta agggtgagtc gttgggggtc ggatataatg
    10381 ctggaagcgc ataggtcgac acccactatc atcgctgata ccgaactaca aacagcggat
    10441 tggctatcac ttatcgaaac ttgggataat catagctcag ggcttgtagg ggcgatgcct
    10501 tccgtgatgg gagcttttgg tagttctgac aaacagctag aagcactggc tagtatcgcc
    10561 cagcaatacc aagtacccta tgccacacac ctagcaccgc taaaaaatga acgtgctgaa
    10621 gttgaacgcg tatttggtcg ctcgcctatc gcgcgctttg atgccatggg attacttact
    10681 gacaagctat tagctgtgca cacagcttat gcaagcgagg atgagtatca acgccttgtt
    10741 gaaacgggtg ttaatatctg ccattcacca gcgcattacg gcatgcttgg tgaggcaaca
    10801 atcagtgaaa ctgggcagat agggcgtttt ttaaaagatg gtgtatgggt ttctagcagt
    10861 accgatggcg atatctcgtt tatcggtggt atgtgtgaag caatgcgggg agcacacctt
    10921 ggccacaacg aagcacaaaa ttgtaatacc gtctgcccac ctaccttagc cttaaaaaca
    10981 gcgtctttat atgcagcaaa agcgttgggg tgggagcaac aaattggctc aattgaagtg
    11041 ggtaagcagg cagatttagt gctagtgaac aatgatgact atcgctacaa gctttctggt
    11101 catccactga gaacttttat tgttacaggg tcaagtcttg atgtagattc tgtaatggtg
    11161 gctggtgatt tagtggtgaa agcagggcgt agtactcggt ttgatgagca cgagcttttt
    11221 gaggattatc ttaaagcggt ggcgagcgct cgcaaacgta ttggaggtcc agcatgaatc
    11281 aaacgcgatt agcgacttgg tttttgctat tagcaagtac catgacggtg cttgctagtg
    11341 cgactctagc gccagcatta ccagcgatga cagtggcttt ctcaggtatt gaacatgctg
    11401 cattttggac caaaatgacc ttagcattac ctggtctcgt gatagcactt tgtgcgccaa
    11461 tagcaggaag tttagtcgac aaatggaata gtcaaaaatt actagtgaac gcactaatat
    11521 tgttcgtact tagtggtgga ctaggctatt actggcagga ttcgttgtgg ctgatccttg
    11581 gtagtcgagc gatgatggga gtcgctgttg cttttattat ggtgagtggc acaacgatcg
    11641 caggacgtta cttcgaaggg cccaaatttt ctcagtttat gggattgcaa gctgcctttg
    11701 gtggctttgg tggcgtacta ttcttggcgc ttgcaggttt tttagcagag cagcattgga
    11761 cctgggtttt tgctatttac tcactcgcac ttttggtact accaggggta tggctttggg
    11821 taaaggtgcc gccagttagc ctgccaccac aaactcaatc actaccaagt agttggatga
    11881 accgaacttt tttgctgtgc tgtacactag cactggtaga aattatagta ctgtatggat
    11941 taacgattca tttgcctttt tatttgtctg ctcagcaagc atcagccagc actataggct
    12001 tagtaatagc gggcttttta ttcgcgatgt catgcgtctc tatgggttat ggtcgggtta
    12061 gacagtactt gaatattaag caagcacatt tagttggctg gcttattgtt gcaactggat
    12121 tttcgttact tggttttgta aatactttta gtagcatcgt ttgtgctagc tttgtaatcg
    12181 gcgcgggtct tggccttatt cgaccaaacc tagtagtttg gctattcgaa tttgttccgc
    12241 caccgatgcg gggaaaagcg atggggatta tgacaacctg ttactttacc gggcaattta
    12301 ttagccctgt gttgtttgaa ccgatgatag ccagcctagg gtttcagtta ttcttcattt
    12361 atctagcaat gactatcgct tcagttgcag tcttggtggc agggatttat ctattaaatc
    12421 aaaaaacatg gttaaagcaa ctcgactaat tactttaacc tgaagttgct cccagagtgt
    12481 cgctgcgatg cgcttttaca aactgcatcg gagtgacacc gaattgcttt ttgaatgcag
    12541 caataaagtt ggcaacatgg tcataatgtg ccaaatatgc cgcttctcca atcgacaagc
    12601 cttcaactaa caacgcatgt cgtgccttat tgagcttgct ttgtttaacg tactgttttg
    12661 gtgttgtgtt aaagctgcgt ttaaagaaac gctgtaagct gcttaagctc atagcaagct
    12721 ctaatgcgac ctgctcaaca ctttttcctt ccgacaataa atcgatgatc ttattttttt
    12781 tgacctctat atctgagtga aagggctgtt tatacatcac gtggctctgg ttactactgt
    12841 ctatcaagtg gggaagaaag cagctcaacc attgcatcac aacaccttca cactccaaac
    12901 gttcagctaa gcttggttgt tttggggttt gaagcagttg ttgtgacagc gaaattgctt
    12961 ttgcttgtag cggtaactca atcactttcg tggtggtaaa tagagattga tattgctgtg
    13021 acagtctcgc aacgagccag cgaggactga cgctcagtgt gaccttttct acttcttggt
    13081 gtttttgcag atagcgagtg aaaggctcgg gctgagtgat gaggttgaca aatgcctgag
    13141 gtgcgttttc agcgctgaaa cggtaatatt tattcgcaac tctaaattcc acaacgcctt
    13201 taagcaatac agtaatgttc acacttggtt gcaattctgc acagctgcta gtttgctgct
    13261 gctcgataca ttgacaggta tgaagttgaa ggtcatcgct caatggcaaa aaagagagtc
    13321 gaccaatcgc aataagttgc tcaatatcaa agccgttgat acggcagttt aatccgctcg
    13381 ccaataacaa cttgttgttg agctccgcgt tggaaatatt cccttccata gtaagtgcca
    13441 caattttcta aaacgcataa tagattgacg attttgcata actaatattc aaaatgcaac
    13501 aaaagtaaaa gtgtacaata ttgcgagtga ttcgtattaa catcaaaatt taaaggatat
    13561 ttatgttggt ttctcgcact aagcagttag gctttcgccc ttcaaccctc gctttagcac
    13621 tcgcgggggt actatcagca ccagctttgg ctgactcagg tgatgcaggt aaaatagaaa
    13681 gaatagaagt ttggtcaact gcggtaaaaa cttctgcact gtatttaaaa gaacaagaaa
    13741 ttgcggataa acaagcagac catatttctg atttacttcg ctctattcct ggcattgatg
    13801 tcggtggtgc acactcgctg aaccagcgaa tcactatccg tagtatggat gacaaagatc
    13861 ttaaaatttc aatagatggt gcagcacaaa atacttacat gtaccaccac atgggtaacc
    13921 ttcaaattca tgctgatatt ctaaagtcag tagatattga aacgggcact aactcagtaa
    13981 ttaacggcgg tcttggtggc tcagtgcggt tcgaaacaaa agaagcacgt gaattgcttt
    14041 ctggtgacga tcgctttgcc gttcgtgttt cagcaggggc tgcagacaat gccgggcata
    14101 attactcggt aacgggtttt ggtttgttga ccgatgatgt cgactttctt gcatattaca
    14161 accatgttga gcgtgataat tacgaagtgg gtggtggcaa gatcttagac caaaacgatg
    14221 aagtcgttgc tggtactgat ggtgaggtaa aaggtcttga aggtgaagtt tctgatgcgc
    14281 taattaaatt tggttggaat attaataaca accaacgtct tgcactgagt ttcgagactt
    14341 ataaagatga aggtgactac agttatcgcc ctgatatggg cttagcgaca gatgttgcta
    14401 ttaccaatag cttgcagatc cctttgttat ggccgacaga gtttacaaga gacacaatta
    14461 cgctgagcta tgatcttaac tggggcgaaa actcttcatt aaaggcgagc ttatattcca
    14521 ataccagtga actttatcgc gacgaatcag gttggtctat gtctccttct cctaggttcc
    14581 aagcgtacgc tggagaagtg acgggtgaag ctgaaaatac ggggctaaat ttgcttggtg
    14641 aaacactggt aacagggctt tttgccgaag aagagcatac cttcacttat ggtgcagatt
    14701 atgtgaagca cgatactcag tacgatctag ctattgcatc aggtggttca aagcactctg
    14761 aagaaagtgc gaaaaatcta gcctttttca ttcaggacaa aattgatttt ggtaatggtt
    14821 tggcggttat tcctggtgtt aggcacgata gctacgatat agattcaggc acagtgaaca
    14881 atgattattc ggaattttct ttatcattgg ctgctgagta tcaatttaat gaaaatctaa
    14941 tggtgaagtt aagttcgact gagctattta aaggcccaga gataagtgag gtatttgttg
    15001 gtgctggttt aggtgataac cgcaatcaag acattgaagc ggaaacgggc gttaataccg
    15061 agttttcgat tgcctatgat ggtgaagttg ggaccaacaa gacactccgt ttaggcgcaa
    15121 cgatattcag aacgcaaatt gacgactata tttttgataa tgcagcatta cctggcggcg
    15181 caccccgtga aacgtggaaa gacaacgtcg gtgatatgac tgtagatggg tttgaagctt
    15241 atgctggttt tgcgcttgat gcgttttctt tccagttaac ttactctaac tctgagagtg
    15301 aacttgatgc atttacacag tatgcctcgt tagatggtgc gcgtttagag cgagagcagg
    15361 gcgatactat ttcagctgac tttacttacc aaattacggc gtataacttg acgctgaatt
    15421 gggattttat ggttgttgat gatgtagcac aaggtaccga tctctatggc gcagacttag
    15481 acaacagtaa agatggcttc accgtgcata atatcagcgc acactggcag cctgaatcag
    15541 tacctagttt gcatgttcgt ctaggtgtag ataatttgtt tgatgagttt tactcttcac
    15601 aatcttctaa aaacggggtt tcttttcacc cagtatttgg tgaattattc ctaatggact
    15661 atgaaccagg aagaaacatt aaagcaagtg tatcttacca gttttaactg ggttagcacc
    15721 tgctgttata aacgtgtaat accgcaggta agttaattcc gaaaattaat atgcccagta
    15781 aaatgctggg catttttatc gttttgtagt taagcagttg cgcgcatctt gatttggcta
    15841 agttgatggt aagtctgatt ggattcaagc gcatttagca tggttgaaag ttttgcttca
    15901 gtttctagcc attcagcagt cggatcagaa gctgcgacaa taccagctcc cgcaaatagt
    15961 ctggcatgat tcgcacttat ctcaccactg cggataacca ccacccattc accatttcca
    16021 tgtttgtcac accaaccaac gatccctgag aaaaagtcac gactttctcc ttctagtgag
    16081 tggatccaag gataggcatc ggcagtcggt ttaccgcata gtgcaggcgt agggtgtaga
    16141 cggttggcta gtgctaatac atgagtgtta gcgtcggcga gttgaccttc aacctctgtg
    16201 gacaagtgcc acatcgttgc tgtgctcagc aatgagggcg agctgggtat ggtaagcttt
    16261 tgacaccaag gttgtaaaac ctgtgacatt tctaccaata caacagcatg ttcataccga
    16321 tctttctttg atgccattaa tgcttgtttg agtttaaact ccgtagcctt atcatttgaa
    16381 cgtttagctg agcctgcaag gggattactg agaaccgctt gcccatgttt gcgtaagagt
    16441 aattctggac tcacgcccat caaggtggaa ttttgttccg ttggaaaaga gaagtgaaaa
    16501 ccgctagggc tttgggttaa taaatttgtc agaatggttt ccggtgaaag tggtgcatca
    16561 aaatagaggt ctacttgttt gccaagaaca attttttcta attgttcttt ggcaaatagc
    16621 tttttggctt cacgtatcgc actctcatag tgatgctgtg attgtctatg cgagatacgc
    16681 tcaagcgatg gcatagtgtc ctgcttaagc gtgcctttgg cgtttaacca ttgcttgaaa
    16741 gtctctttat catattggtg cgcttgctca gttacaataa attgagcttg ttgagttttg
    16801 caaaatggta gcacaccgaa tgctatcgca tcattgctgc tgcactttgc tagctctcgt
    16861 tttaactggc tcactaatac atcgtgatct tttatctgag tagtgaacct atgatgcggc
    16921 cgttgagtta cgaggcaccg tgtgcccgaa acaaaaaaag cttgttgtga taagtcaatg
    16981 gactcaagcg ttgtattggt ggattgcaaa gcagatattt cactcaacat
//
