LOCUS       BGC0002493              6076 bp    DNA     linear   BCT 28-JAN-2014
DEFINITION  Paracoccus denitrificans PD1222 chromosome 1, complete sequence.
ACCESSION   BGC0002493
VERSION     BGC0002493
KEYWORDS    .
SOURCE      Paracoccus denitrificans PD1222
  ORGANISM  Paracoccus denitrificans PD1222
            Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
            Paracoccaceae; Paracoccus.
COMMENT     URL -- http://www.jgi.doe.gov
            JGI Project ID: 3634481
            Source DNA and bacteria available from Stephen Spiro
            (stephen.spiro@utdallas.edu)
            Contacts: Stephen Spiro (stephen.spiro@utdallas.edu)
            Paul Richardson (microbes@cuba.jgi-psf.org)
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            There is an unverified repetitive structure in the vicinity of
            complement(2413304-2418104)
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:49:50
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6076
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6076
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1852:6076]"
                     /cutoff="30000"
                     /detection_rule="(VibH_like or Cy_tandem or
                     (cds(Condensation and AMP-binding) and ((IBH_Asp and not
                     SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or
                     CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD
                     and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox
                     and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))"
                     /neighbourhood="20000"
                     /product="NRP-metallophore"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1853..6076
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="30000"
                     /detection_rule="(VibH_like or Cy_tandem or
                     (cds(Condensation and AMP-binding) and ((IBH_Asp and not
                     SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or
                     CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD
                     and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox
                     and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))"
                     /neighbourhood="20000"
                     /product="NRP-metallophore"
                     /protocluster_number="1"
     cand_cluster    1..6076
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(VibH_like or Cy_tandem or
                     (cds(Condensation and AMP-binding) and ((IBH_Asp and not
                     SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or
                     CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD
                     and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox
                     and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))"
                     /kind="single"
                     /product="NRP-metallophore"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..6076
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002493"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6076
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRP-metallophore"
                     /region_number="1"
                     /rules="(VibH_like or Cy_tandem or (cds(Condensation and
                     AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or
                     TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or
                     (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or
                     PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like))
                     or Lys_monoox or VbsL)))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1206..1862
                     /locus_tag="Pden_2383"
     CDS             1206..1862
                     /EC_number="3.3.2.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR000868"
                     /db_xref="InterPro:IPR003881"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:isochorismatase (Score: 272.4; E-value: 5.1e-83)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Pden_2383"
                     /note="PFAM: isochorismatase hydrolase; KEGG: bsu:BG11241
                     isochorismatase (2,3 dihydro-2,3 dihydroxybenzoate
                     synthase)"
                     /product="Isochorismatase"
                     /protein_id="ABL70473.1"
                     /transl_table=11
                     /translation="MALPGIAPYAPPTEAELPKARGPWQPDRDRLALLVHDMQRYFCRV
                     YPEGAPLAPVIGNIARLIHAARAAGVPVFYTAQKGNQFRPDRGLQADLWGPGMSATPEH
                     EEILPELASATGDIVLVKHRYSAFQRSNLEQLMRARGRDQLMICGVYAHIGCLATAVEA
                     FQRDIEAFAVADAQADFSREKQDMAMEWIAATCGVPLSTRQALEALGTHGVKSCA"
     sig_peptide     1853..1933
                     /locus_tag="Pden_2384"
                     /note="Signal predicted by SignalP 2.0 HMM (Signal peptide
                     probabilty 0.884) with cleavage site probability 0.820 at
                     residue 27"
     gene            1853..2623
                     /locus_tag="Pden_2384"
     CDS             1853..2623
                     /NRPS_PKS="Domain: PKS_KR (9-161). E-value: 9.7e-13. Score:
                     40.3. Matches aSDomain: nrpspksdomains_Pden_2384_PKS_KR.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR001509"
                     /db_xref="InterPro:IPR002198"
                     /db_xref="InterPro:IPR002424"
                     /db_xref="InterPro:IPR003560"
                     /db_xref="InterPro:IPR013120"
                     /db_xref="InterPro:IPR013968"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRP-metallophore: EntA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     217; E-value: 4.9e-66)"
                     /gene_kind="biosynthetic"
                     /locus_tag="Pden_2384"
                     /note="PFAM: NAD-dependent epimerase/dehydratase;
                     short-chain dehydrogenase/reductase SDR; Male sterility
                     C-terminal domain; KR; KEGG: hch:HCH_06575 short-chain
                     alcohol dehydrogenase-like protein"
                     /product="short-chain dehydrogenase/reductase SDR"
                     /protein_id="ABL70474.1"
                     /sec_met_domain="EntA (E-value: 2.4e-105, bitscore: 339.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 2e-35, bitscore:
                     110.5, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLTGFQGKTAIVTGAAGGIGRAVVAALLDAGATVVAADTESALA
                     GNPVAGTENRVLDVRDGDAVEALVAEVEAAHGPLALGVHAAGVLAIGPLLELPPEEWER
                     VIGVNARGTFHVTRAFGRAMVRNGGGAIVAVSSNAAGIPRMNMGVYAASKAAATMLVRC
                     LGLELAAQGVRCNIVAPGSTLTPMQTGMWTEDGGAGERAVIEGSLDAYRTGIPLKKLAT
                     PEDIAGAVMFMLSDQAGHVTMADLYVDGGATLRA"
     aSDomain        1880..2335
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Pden_2384_PKS_KR.1"
                     /evalue="9.70E-13"
                     /label="Pden_2384_PKS_KR.1"
                     /locus_tag="Pden_2384"
                     /protein_end="161"
                     /protein_start="9"
                     /score="40.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TAIVTGAAGGIGRAVVAALLDAGATVVAADTESALAGNPVAGTEN
                     RVLDVRDGDAVEALVAEVEAAHGPLALGVHAAGVLAIGPLLELPPEEWERVIGVNARGT
                     FHVTRAFGRAMVRNGGGAIVAVSSNAAGIPRMNMGVYAASKAAATMLV"
     CDS_motif       1886..1954
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Pden_2384_0001"
                     /evalue="2.80E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="Pden_2384"
                     /protein_end="34"
                     /protein_start="11"
                     /score="16.8"
                     /tool="antismash"
                     /translation="IVTGAAGGIGRAVVAALLDAGAT"
     CDS_motif       2204..2260
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Pden_2384_0002"
                     /evalue="1.30E-01"
                     /label="PKSI-KR_m1"
                     /locus_tag="Pden_2384"
                     /protein_end="136"
                     /protein_start="117"
                     /score="5.0"
                     /tool="antismash"
                     /translation="RAFGRAMVRNGGGAIVAVS"
     gene            complement(2663..2905)
                     /locus_tag="Pden_2385"
                     /pseudo=""
     gene            complement(2917..4539)
                     /locus_tag="Pden_2386"
     CDS             complement(2917..4539)
                     /NRPS_PKS="Domain: AMP-binding (30-438). E-value: 2.3e-77.
                     Score: 252.1. Matches aSDomain:
                     nrpspksdomains_Pden_2386_AMP-binding.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR000873"
                     /db_xref="InterPro:IPR011963"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     424.8; E-value: 6.2e-129)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Pden_2386"
                     /note="TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; PFAM:
                     AMP-dependent synthetase and ligase; KEGG: bms:BRA0015
                     2,3-dihydroxybenzoate-AMP ligase"
                     /product="2,3-dihydroxybenzoate-AMP ligase"
                     /protein_id="ABL70475.1"
                     /sec_met_domain="AMP-binding (E-value: 5.6e-86, bitscore:
                     276.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEFSPWPQDLAQRYREAGYWIDQPLTHILDAQLAANPEAEALVC
                     GSRRFSYADLDRLSCNLAARLAQQGLGHGDTALVQLPNVAEFFVAFFALLRIGVAPVNA
                     LFSHRRLELAEYVRQIAPRVVIASRQHELFRDDAFAEELRAGGVAHLLLLGEDDPQRSL
                     DHWLTHGGDLPAGAGPTPGGEVAFFQLSGGSTGTPKLIPRTHNDYDYSIRASVQICGVT
                     SATRFLCALPAGHNFTMSSPGALGVFHAGGTVVMASSPEPLTCFQIVARERITMAPLVP
                     PAVALWLRAVEEEPARRAMLDSLELMLVGGASFAEATARRVPELLGCRLQQVFGMAEGL
                     VNYTRLDDPPEVIFTTQGRPISPDDEVRIVDEDGCDVPDGTPGALATRGPYTFRGYYRS
                     PEHNAKSFDAEGFYYSGDVVVRQGGNLRVVGRIKDQINRGGEKIAAEEIENLLVRHPDI
                     THAALVAAPDAHLGEKSCAFLVLREGSGKLSSPALRRHLLELGVAEYKLPDRFRFVPEL
                     PLTAVGKIDKRRLRDSLVAETAA"
     aSDomain        complement(3226..4449)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Pden_2386_AMP-binding.1"
                     /evalue="2.30E-77"
                     /label="Pden_2386_AMP-binding.1"
                     /locus_tag="Pden_2386"
                     /protein_end="438"
                     /protein_start="30"
                     /score="252.1"
                     /specificity="substrate consensus: diOH-Bz"
                     /tool="antismash"
                     /translation="DAQLAANPEAEALVCGSRRFSYADLDRLSCNLAARLAQQGLGHGD
                     TALVQLPNVAEFFVAFFALLRIGVAPVNALFSHRRLELAEYVRQIAPRVVIASRQHELF
                     RDDAFAEELRAGGVAHLLLLGEDDPQRSLDHWLTHGGDLPAGAGPTPGGEVAFFQLSGG
                     STGTPKLIPRTHNDYDYSIRASVQICGVTSATRFLCALPAGHNFTMSSPGALGVFHAGG
                     TVVMASSPEPLTCFQIVARERITMAPLVPPAVALWLRAVEEEPARRAMLDSLELMLVGG
                     ASFAEATARRVPELLGCRLQQVFGMAEGLVNYTRLDDPPEVIFTTQGRPISPDDEVRIV
                     DEDGCDVPDGTPGALATRGPYTFRGYYRSPEHNAKSFDAEGFYYSGDVVVRQGGNLRVV
                     GRIKDQINR"
     CDS_motif       complement(3319..3408)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Pden_2386_0001"
                     /evalue="4.50E-04"
                     /label="NRPS-A_a6"
                     /locus_tag="Pden_2386"
                     /protein_end="407"
                     /protein_start="377"
                     /score="12.7"
                     /tool="antismash"
                     /translation="PDGTPGALATRGPYTFRGYYRSPEHNAKSF"
     gene            4727..6076
                     /locus_tag="Pden_2387"
     CDS             4727..6076
                     /NRPS_PKS="Domain: Condensation_Starter (10-314). E-value:
                     5e-62. Score: 201.8. Matches aSDomain:
                     nrpspksdomains_Pden_2387_Condensation_Starter.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR001242"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRP-metallophore: VibH_like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     284.5; E-value: 3e-86)"
                     /gene_kind="biosynthetic"
                     /locus_tag="Pden_2387"
                     /note="PFAM: condensation domain protein; KEGG:
                     bmb:BruAb2_0017 hypothetical enterobactin synthetase,
                     component F"
                     /product="condensation domain protein"
                     /protein_id="ABL70476.1"
                     /sec_met_domain="Condensation (E-value: 4.3e-29, bitscore:
                     89.6, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="VibH_like (E-value: 2.7e-177, bitscore:
                     577.6, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHHQAEGLWLPLTLAQMDFWEEFRFHPGEAVSTVAHAIELRGVA
                     DPEALAGAITATIAETDIMALRFGLGADGRPVQRVDPTQRPRLRHLDLSDRADAREHAL
                     ALMQADVDTALDLLDQPIAAQWLIRLAEGHYLWFNRGHHIALDGYAMGLIERRCAQLYA
                     ALAKGADPGQPLRPLADYLNEETDYRASPRHAADGRHWAEVLAARPRPRVLCKGSEDYP
                     AEALCAAHDLSALRPALMRQAQAVGMGWPDLLTVLCSAWAALHLRDGSAERLADDHGRI
                     AMPVWLPYMSRMGSVAVSVPALVVNILPLDVTLHPAEPLGEALARLGGRLRKLRRHGRY
                     RIEQIAEDYGMEPRSRFFFSPLINVLPFDEARFAGCETARHVLSNGPGDGFNITIRGDG
                     EAQGLELLVEADPTLTPPEDFARLSDGLPRFLSAALVPENLPLPLAGLPG"
     aSDomain        4757..5668
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Pden_2387_Condensation_Starter.1
                     "
                     /domain_subtypes="Condensation_Starter"
                     /evalue="5.00E-62"
                     /label="Pden_2387_Condensation_Starter.1"
                     /locus_tag="Pden_2387"
                     /protein_end="314"
                     /protein_start="10"
                     /score="201.8"
                     /tool="antismash"
                     /translation="LPLTLAQMDFWEEFRFHPGEAVSTVAHAIELRGVADPEALAGAIT
                     ATIAETDIMALRFGLGADGRPVQRVDPTQRPRLRHLDLSDRADAREHALALMQADVDTA
                     LDLLDQPIAAQWLIRLAEGHYLWFNRGHHIALDGYAMGLIERRCAQLYAALAKGADPGQ
                     PLRPLADYLNEETDYRASPRHAADGRHWAEVLAARPRPRVLCKGSEDYPAEALCAAHDL
                     SALRPALMRQAQAVGMGWPDLLTVLCSAWAALHLRDGSAERLADDHGRIAMPVWLPYMS
                     RMGSVAVSVPALVVNILPLDVTL"
     CDS_motif       4820..4933
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Pden_2387_0001"
                     /evalue="7.30E-08"
                     /label="C2_starter_024-063"
                     /locus_tag="Pden_2387"
                     /protein_end="69"
                     /protein_start="31"
                     /score="25.1"
                     /tool="antismash"
                     /translation="VSTVAHAIELRGVADPEALAGAITATIAETDIMALRFG"
     CDS_motif       5150..5212
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Pden_2387_0002"
                     /evalue="6.60E-09"
                     /label="C3_starter_134-155"
                     /locus_tag="Pden_2387"
                     /protein_end="162"
                     /protein_start="141"
                     /score="28.6"
                     /tool="antismash"
                     /translation="HHIALDGYAMGLIERRCAQLY"
     CDS_motif       5585..5656
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Pden_2387_0003"
                     /evalue="1.00E-06"
                     /label="C5_starter_265-294"
                     /locus_tag="Pden_2387"
                     /protein_end="310"
                     /protein_start="286"
                     /score="21.3"
                     /tool="antismash"
                     /translation="LPYMSRMGSVAVSVPALVVNILPL"
     CDS_motif       5714..5821
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Pden_2387_0004"
                     /evalue="1.10E-08"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="Pden_2387"
                     /protein_end="365"
                     /protein_start="329"
                     /score="27.7"
                     /tool="antismash"
                     /translation="RLRKLRRHGRYRIEQIAEDYGMEPRSRFFFSPLINV"
ORIGIN
        1 atgaacatcc gcctgtccga ggcgccgaaa aagacggcgt cgcccgtgcc tgatccggtt
       61 ctgttcggtt tcgacggccc ctcgggtcgc atcctgggcc aaggccccgc acgtcgcatc
      121 gcccccggca acgccgcgac gctggagacg cgggtgacgg cggcccttgc cggggccgcg
      181 ccggacgaga tgatcggcgg cgccctgccc ttcgaccgcg aggccgggga ctgcctgtgg
      241 caatgccgcc gcagcgaggc gcccgccccc ggcggcatca cgccgcaggg cctgccgctg
      301 ccgggctggc gcctgactgc cgagcccgcg gcgcgcgact atgccgatgc cgtggcccgc
      361 gcgctcgaca tcatggccga agaggcgggc agcgaggatg cgctggaaaa gatcgtgctg
      421 gcgcgcacgc ttctggtcga atccgccgcg ccgatcccgg tcgaggcgct gatggcgcgg
      481 ctgcatgccg accgcagcgt gaccgccttt cgcgtggcgc tgcccgccgg acccgaccat
      541 ccgcagggtc gcgcgctgat cggcgcgacg cccgaactgc tgatcgagaa gcggggccgg
      601 cgcatcgcat cgcatccgct ggcgggctcg gcgcgccggc tgtccgatcc ggccgcagac
      661 cgcgccgccg ccgaagccct gtccggttcg gccaaggacc accgcgaaca tgcgctggtg
      721 gtcgagtcga tcctggacac gctggcgccc ttctgccgcg aactgggctg ccccgagggc
      781 acctgcatca cctcgacccg cagcatgtgg catctgggca cccggatcga gggccggctg
      841 aaggacgagg gcacgccctc ggtcgtgctg gcctcggcgc tgcatccgac gccggcggtc
      901 tgcggcgtgc cgatggcgcg ggcggcgggc ttcatcaagg cccttgaacc cgtggcgcgg
      961 gatttctatg ccggcgcggt cggctggtgc gacgcaagcg gcgacggcgc ctggcatgtc
     1021 gccatccgct gcgccgagat cagcggccgg cgggcgcggc tttacgcggg cgcggggatc
     1081 gttcccggct cggacccgat ggccgaggcc gccgagaccg gcgccaagtt cggcgcgctt
     1141 ctggccgccc tgggcctgcc gcaggacgcg gcgctgcagg gcgtgccatg caacggagag
     1201 acctgatggc cctgcccggc atcgccccct acgccccccc gaccgaggcc gaactgccca
     1261 aggcgcgcgg tccctggcag cccgaccgcg accgtctggc gctgctggtc cacgacatgc
     1321 agcgctattt ctgccgcgtc tatcccgagg gcgcaccgct ggcgccggtg atcggcaata
     1381 tcgcgcggct gatccatgcg gcgcgggcgg cgggcgtgcc ggtcttttat accgcgcaaa
     1441 agggcaatca gttccgcccg gatcgcgggc tccaggccga cctgtggggg ccgggaatga
     1501 gcgccacccc cgagcatgag gagatcctgc ccgagttggc ctcggcgaca ggcgacatcg
     1561 tgctggtcaa gcatcgctac agcgccttcc agcgctccaa cctggagcag ctgatgcgcg
     1621 cgcgcggccg cgaccagctg atgatctgcg gcgtctatgc ccatatcggc tgcctcgcca
     1681 ccgccgtcga ggcgttccag cgcgacatcg aagcctttgc cgtggccgat gcgcaggcgg
     1741 atttctcgcg cgagaaacag gacatggcca tggaatggat cgccgccacc tgcggcgtgc
     1801 cgctgtccac ccggcaggcg ctggaggcgc ttggcacgca tggggtgaag tcatgcgcct
     1861 gaccggattt cagggcaaga ccgccatcgt caccggcgcg gcaggcggca tcggccgcgc
     1921 ggtggtcgcc gcgctgctgg acgccggggc gacggtcgtc gccgccgata ccgaatccgc
     1981 gctggccggc aatcccgtcg cggggaccga gaaccgggtg ctggacgtgc gcgacggcga
     2041 tgcggtcgag gccctggtgg ccgaggtcga ggccgcgcat ggcccgctgg cgcttggtgt
     2101 ccatgcggcc ggcgtgctgg ccatcgggcc gctgctggag ttgccgcccg aggaatggga
     2161 gcgggtgatc ggcgtgaacg cccggggcac cttccacgtc acccgcgcct ttggccgggc
     2221 catggtgcgg aacggcggcg gcgccatcgt cgcggtcagt tcgaacgcgg cggggattcc
     2281 gcgcatgaac atgggcgtct atgccgcgtc caaggcggcg gcgaccatgc tggtccgctg
     2341 cctggggctg gaactggccg cccagggcgt gcgctgcaac atcgtcgcgc cgggctcgac
     2401 gctgacaccg atgcagaccg gcatgtggac cgaggacggc ggcgcgggcg agcgcgcggt
     2461 gatcgagggc agcctggacg cctatcgcac cggcatcccg ctgaagaagc tggcgacacc
     2521 cgaggacatc gccggcgcgg tgatgttcat gctgtcggac caggcgggcc atgtcaccat
     2581 ggccgacctt tacgtcgatg gcggcgcgac gctgcgcgcc tgaccctttg tcggggccgg
     2641 cctgacacgg ccggcccatc gttcacaacc gcgacgaaat cagcgcccac caattgtcca
     2701 gcgacggctc gcgggccagt tcctcgaagc tggcctcgat gcccatctgc ttgagctccg
     2761 acgaaaaggt catcaccgcg atcgagtcga gcccgtagag gacaaggttc tcacccggct
     2821 cgatctcgca atcgtcctcg atcatcttct gaacacgggc ggccagccat tcggggctga
     2881 gatcggtctt ttcggcaata tccatcattc gtcctttcag gcggcggttt cggccacgag
     2941 gctgtcgcgc aggcggcgct tgtcgatctt gcccaccgcc gtcagcggca gttcgggcac
     3001 gaagcggaaa cggtcgggca gcttgtattc ggccacgccc agttccagca ggtgccggcg
     3061 cagcgccggc gaggaaagct tgcccgaccc ctcgcgcagc accaggaagg cgcagctttt
     3121 ctcgcccaga tgcgcgtcgg gggcggcgac cagcgcggca tgggtgatgt cggggtggcg
     3181 caccagcagg ttctcgattt cctcggcggc gatcttttcg ccgccccggt tgatttggtc
     3241 cttgatgcgg ccgacgacgc gcaggttgcc gccctgccgc accaccacgt cccccgaata
     3301 gtagaacccc tcggcgtcaa aggacttcgc attgtgctcg gggctgcggt agtagccgcg
     3361 gaaggtatag gggccgcggg ttgccagcgc gccgggcgtg ccgtccggca cgtcgcagcc
     3421 gtcctcgtcg acgatgcgca cctcgtcgtc ggggctgatc ggccggccct gggtggtgaa
     3481 aatcacctcg ggcggatcgt ccagccgggt atagttcacc agcccctcgg ccatgccgaa
     3541 gacctgctgc aggcggcagc ccagcagttc gggaacgcgc cgcgccgtcg cctcggcgaa
     3601 actggcgccg ccgaccagca tcagctccag gctgtccagc atggcgcggc gggcgggctc
     3661 ttcctcgacc gcgcgcagcc acagcgcgac ggcgggcggc accagcggcg ccatggtgat
     3721 ccgttcgcgc gccacgatct ggaagcaggt cagcggttcg gggctggagg ccatgaccac
     3781 cgtgccgccg gcatggaaca cccccagcgc gccgggcgag ctcatggtga agttgtgccc
     3841 cgccggcaag gcgcaaagaa agcgggtggc cgaggtcacg ccgcagatct ggacgctggc
     3901 gcggatgctg tagtcgtaat cgttatgggt gcgcgggatc agcttgggcg tcccggtgct
     3961 gccgcccgac aactggaaga aggcgacctc gccgcccggg gtcggccccg cccccgccgg
     4021 caggtcaccg ccatgggtca gccagtgatc caggctgcgc tgggggtcgt cctcgcccag
     4081 cagcagcaga tgcgcgacgc cacccgcccg caattcctcg gcaaaggcgt cgtcgcggaa
     4141 cagctcgtgc tggcgcgagg caatgaccac gcgcggcgcg atctggcgga catattccgc
     4201 aagctccagc cggcgatggc tgaacaacgc gttcaccggc gccacgccga tccgcagaag
     4261 cgcgaagaag gcgacgaaga actcggccac gttcggcaat tgcaccagcg ccgtatcgcc
     4321 atggcccagc ccctgctgcg ccagcctggc ggccaggttg cacgacagcc ggtccagatc
     4381 ggcatagctg aagcggcgcg aaccgcagac cagcgcctcg gcctcggggt tggcggccag
     4441 ctgcgcgtcc aggatatggg tcagcggctg gtcgatccaa taaccggcct cgcgatagcg
     4501 ctgcgcgagg tcttgcggcc aagggctgaa ttcaatcatg cggatacctg ttctgacggc
     4561 aatgacccgc tcagggcacc tgcggcacct tgacgggtgt taatagttga gtatttttat
     4621 cccgctttct ctcttgaagc aagccagccg catccttccc gggacgcaac caagccagcc
     4681 ccctctcgtt cagaatgcca gaccctgcga cagggataag gatttcatga cgcatcatca
     4741 agccgagggg ctttggctgc ccctgacgct ggcccagatg gatttctggg aagagttccg
     4801 gttccacccc ggcgaggcgg tctcgacagt cgcccatgcc atcgagttgc gcggggtcgc
     4861 ggaccccgag gcgctggccg gcgccataac ggcgaccatc gccgagaccg acatcatggc
     4921 gctacgcttc ggccttggtg ccgatggccg gccggtgcag cgggtcgatc ccacgcagcg
     4981 gccccggctg cgccacctgg acctgtcgga ccgcgcggat gcgcgcgagc acgcccttgc
     5041 gctgatgcag gcggatgtcg atacggcgct tgacctgctg gaccagccca tcgcggcgca
     5101 gtggctgatc cggctggccg aggggcatta cctgtggttc aaccgcgggc atcatatcgc
     5161 gctggacggc tatgccatgg gcctgatcga acggcgctgc gcccagcttt acgccgcctt
     5221 ggccaagggc gcggaccccg gccagccgct gcgcccgctg gccgactatc tgaacgagga
     5281 aaccgactat cgcgccagcc cgcgccatgc cgccgacggc cggcactggg ccgaagtgct
     5341 ggcggccagg ccccgcccgc gcgtgctgtg caaggggtcc gaggactatc ccgccgaggc
     5401 gctttgcgcc gcgcatgacc tgtcggcgct gcgccccgcg ctgatgcggc aggcgcaggc
     5461 ggtgggcatg ggctggccgg acctgttgac ggtgctctgc tcggcctggg ccgcactgca
     5521 cctgcgggac ggctcggccg agcggctggc cgacgaccat ggccgcatcg cgatgccggt
     5581 ctggctgccc tatatgagcc ggatgggcag cgtcgccgtc tcggtcccgg cgctggtggt
     5641 gaacatcctg ccgctggacg tgaccctgca cccggccgaa ccgctgggcg aggcgctggc
     5701 ccggctgggc gggcggctgc gcaagctgcg ccgccacggc cgctaccgca tcgagcagat
     5761 cgccgaggat tacggcatgg agccgcgcag ccgcttcttc ttctcgccgc tgatcaacgt
     5821 gctgcccttt gacgaggcgc gctttgccgg ctgcgaaacc gcgcggcatg tgctgtcgaa
     5881 cggcccgggc gacgggttca acatcaccat ccgcggcgat ggcgaggcgc aggggctgga
     5941 actgctggtc gaggccgatc cgaccctgac cccgcccgag gatttcgccc ggctgtccga
     6001 cggcctgccg cgttttcttt cggcggcgct ggtgccggaa aacctgccgc tcccgctggc
     6061 gggcctgccg ggctga
//
