LOCUS       BGC0002494             12200 bp    DNA     linear   BCT 06-JAN-2000
DEFINITION  Vibrio cholerae vibriobactin gene cluster, complete sequence.
ACCESSION   BGC0002494
VERSION     BGC0002494
KEYWORDS    .
SOURCE      Vibrio cholerae
  ORGANISM  Vibrio cholerae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
COMMENT     On Aug 12, 1999 this sequence version replaced U52150.1.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 17:49:36
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12200
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /db_xref="taxon:666"
                     /mol_type="genomic DNA"
                     /organism="Vibrio cholerae"
                     /strain="Lou15 (El Tor)"
     subregion       1..12200
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="NRPS"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12200
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     protein_bind    111..129
                     /bound_moiety="fur"
                     /gene="vibB"
                     /note="putative"
     gene            111..1155
                     /gene="vibB"
     CDS             274..1155
                     /NRPS_PKS="Domain: PP-binding (223-279). E-value: 1.8e-11.
                     Score: 36.3. Matches aSDomain:
                     nrpspksdomains_vibB_PP-binding.1"
                     /codon_start=1
                     /gene="vibB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:isochorismatase (Score: 340.6; E-value: 8e-104)"
                     /gene_kind="biosynthetic-additional"
                     /note="VibB"
                     /product="isochorismatase"
                     /protein_id="AAC45926.1"
                     /sec_met_domain="PP-binding (E-value: 3.5e-12, bitscore:
                     36.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIPKIASYPLPVSLPTNKVDWRIDASRAVLLIHDMQEYFVHYFD
                     SQAEPIPSLIKHIQQLKAHAKQAGIPVVYTAQPANQDPAERALLSDFWGPGLSEETAII
                     APLAPESGDVQLTKWRYSAFKKSPLLDWLRETGRDQLIITGVYAHIGILSTALDAFMFD
                     IQPFVIGDGVADFSLSDHEFSLRYISGRTGAVKSTQQACLEIAAQHSKLTGLSLRTMQH
                     DVAAALNLSVDEVDVQENLLFLGLDSIRAIQLLEKWKAQGADISFAQLMEHVTLQQWWQ
                     TIQANLHQPCSA"
     aSDomain        943..1110
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_vibB_PP-binding.1"
                     /evalue="1.80E-11"
                     /label="vibB_PP-binding.1"
                     /locus_tag="vibB"
                     /protein_end="279"
                     /protein_start="223"
                     /score="36.3"
                     /tool="antismash"
                     /translation="VAAALNLSVDEVDVQENLLFLGLDSIRAIQLLEKWKAQGADISFA
                     QLMEHVTLQQW"
     regulatory      1164..1207
                     /note="putative rho-independent terminator"
                     /regulatory_class="terminator"
     gene            complement(1256..2887)
                     /gene="vibE"
     CDS             complement(1256..2887)
                     /NRPS_PKS="Domain: AMP-binding (31-439). E-value: 7.3e-79.
                     Score: 257.1. Matches aSDomain:
                     nrpspksdomains_vibE_AMP-binding.1"
                     /codon_start=1
                     /gene="vibE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     434.6; E-value: 6.9e-132)"
                     /gene_kind="biosynthetic-additional"
                     /note="VibE"
                     /product="2,3-dihydroxybenzoate-AMP ligase"
                     /protein_id="AAC45927.1"
                     /sec_met_domain="AMP-binding (E-value: 2.2e-89, bitscore:
                     289.4, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALV
                     GDNQQWRYQAMLERIEQLAAGFTELGLGCGDNVVLQLGNVAEFYLCFFALLRQGIRPIL
                     ALPAHRLAEIRYFCQHSQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQTRVT
                     DPKFIELASCYSASSCQANADPNQIAFFQLSGGTTGTPKLIPRTHNDYAYSVTASVEIC
                     RFDQHTRYLCVLPAAHNFPLSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTA
                     LVPPLALLWMDHAEKSTYDLSSLHFVQVGGAKFSEAAARRLPKALGCQLQQVFGMAEGL
                     VNYTRLDDSAELIATTQGRPISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRA
                     DQHNQRAFNAQGFYITGDKVKLSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPA
                     VHDAALIAISDEYLGERSCAVIVLKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQL
                     PKTSVGKIDKNALRRRFDTLGLALMS"
     aSDomain        complement(1571..2794)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_vibE_AMP-binding.1"
                     /evalue="7.30E-79"
                     /label="vibE_AMP-binding.1"
                     /locus_tag="vibE"
                     /protein_end="439"
                     /protein_start="31"
                     /score="257.1"
                     /tool="antismash"
                     /translation="QQSAERTPNALALVGDNQQWRYQAMLERIEQLAAGFTELGLGCGD
                     NVVLQLGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQHSQAKAYLIDGAQRPF
                     DYQALAQELLACCPTLQTVIVRGQTRVTDPKFIELASCYSASSCQANADPNQIAFFQLS
                     GGTTGTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNFPLSSPGALGVFWA
                     GGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYDLSSLHFVQVGGA
                     KFSEAAARRLPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRPISAHDQLLVVD
                     EQGQPVASGEEGYLLTQGPYTIRGYYRADQHNQRAFNAQGFYITGDKVKLSSEGYVIVT
                     GRAKDQINR"
     CDS             complement(2898..4085)
                     /codon_start=1
                     /gene="vibC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRP-metallophore: EntC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018:isochorismate synthase (Score: 358.1; E-value:
                     1.4e-108)"
                     /gene_kind="biosynthetic"
                     /note="VibC"
                     /product="isochorismate synthase"
                     /protein_id="AAC45925.1"
                     /sec_met_domain="EntC (E-value: 3e-152, bitscore: 496.9,
                     seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVMKREVVGYTTMSEHLIDTQLTRSPFFFASANQSMLGCGVAHAF
                     QQAIPFAELANQAKQLLQQAKRDECDNPLLFGIVPFDPKTPTRFMIPRTLYVSSSPRLN
                     RPAHLTRQVAKLISSPSGEQYKQGVSHLLNMFHHSGLSKVVLSRAIEIATEQEIALPTL
                     LRSLLAINHHGYTFAASLDEQRKLIGASPELLVAKRGNYLISNPLAGSRPRSQDAQENA
                     QRRASLLNTAKDLHEHGLVVEEVERIMSRYCRNLYTPMVPSVIETETMLHLSTLLEGQV
                     SDPEVCALQVAADLHPTPAVCGFPRESAYQAIRELEEFDRGYFTGMVGWCDARGNGEWV
                     VTIRCAEVGSHQMKLFAGAGIVDESLPQSELEETGAKMKTILAAAGIELNDVLTA"
     gene            complement(2898..4147)
                     /gene="vibC"
     protein_bind    complement(4129..4146)
                     /bound_moiety="fur"
                     /gene="vibC"
                     /note="putative"
     protein_bind    4177..4195
                     /bound_moiety="fur"
                     /gene="vibA"
                     /note="putative"
     gene            4177..5041
                     /gene="vibA"
     CDS             4253..5041
                     /codon_start=1
                     /function="vibriobactin biosynthesis"
                     /gene="vibA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRP-metallophore: EntA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     197.5; E-value: 4.2e-60)"
                     /gene_kind="biosynthetic"
                     /note="VibA; required for the synthesis of catechols"
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="AAC45924.1"
                     /sec_met_domain="EntA (E-value: 4.1e-112, bitscore: 363.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.6e-35, bitscore:
                     112.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDMSLVNSKVLLVGSARGIGFSVLEHLLQAGAQVMAADCEWQLL
                     LEQSESLLGRYPDQLTLKKLDLAEPEAVREQVNQWAEQVAGFDHLVCCAGILHVAPLHE
                     MPMEQVSSIFTVNAFGVLACMQGVASSMKARQQGSMVIIGSNAANTPRMSIGAYGASKA
                     ALHMLVKCIGMELAPYGIRCNLVSPGSTRTAMQQQLWTEQYGEAQVIAGDAAQFRLGIP
                     LNKIAEPADIAQAVLFLLSDNAGHITLHDLRVDGGATLDH"
     misc_feature    4315..4316
                     /note="site of Tn5 insertion which inactivates vibA"
     CDS             complement(5053..6363)
                     /NRPS_PKS="Domain: Condensation_Starter (3-281). E-value:
                     5.9e-59. Score: 191.7. Matches aSDomain:
                     nrpspksdomains_vibH_Condensation_Starter.1"
                     /codon_start=1
                     /gene="vibH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRP-metallophore: VibH_like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     231.9; E-value: 2.7e-70)"
                     /gene_kind="biosynthetic"
                     /note="similar to amino terminal region of EntF; required
                     for vibriobactin biosynthesis"
                     /product="VibH"
                     /protein_id="AAD48879.1"
                     /sec_met_domain="VibH_like (E-value: 1.4e-158, bitscore:
                     517.3, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.5e-33, bitscore:
                     104.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLAQKPFWQRHLAYPHINLDTVAHSLRLTGPLDTTLLLRALHLT
                     VSEIDLFRARFSAQGELYWHPFSPPIDYQDLSIHLEAEPLAWRQIEQDLQRSSTLIDAP
                     ITSHQVYRLSHSEHLIYTRAHHIVLDGYGMMLFEQRLSQHYQSLLSGQTPTAAFKPYQS
                     YLEEEAAYLTSHRYWQDKQFWQGYLREAPDLTLTSATYDPQLSHAVSLSYTLNSQLNHL
                     LLKLANANQIGWPDALVALCALYLESAEPDAPWLWLPFMNRWGSVAANVPGLMVNSLPL
                     LRLFAQQTSLGNYLKQSGQAIRSLYLHGRYRIEQIEQDQGLNAEQSYFMSPFINILPFE
                     SPHFADCQTELKVLASGSAEGINFTFRGSPQHELCLDITADLASYPQSHWQSHCERFPR
                     FFEQLLARFQQVEQDVARLLAEPAALAATTSTRAIAS"
     gene            complement(5053..6407)
                     /gene="vibH"
     CDS_motif       complement(5395..5466)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_vibH_0005"
                     /evalue="1.00E-05"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="vibH"
                     /protein_end="323"
                     /protein_start="299"
                     /score="18.1"
                     /tool="antismash"
                     /translation="QAIRSLYLHGRYRIEQIEQDQGLN"
     CDS_motif       complement(5521..5592)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_vibH_0004"
                     /evalue="5.90E-07"
                     /label="C5_starter_265-294"
                     /locus_tag="vibH"
                     /protein_end="281"
                     /protein_start="257"
                     /score="22.0"
                     /tool="antismash"
                     /translation="LPFMNRWGSVAANVPGLMVNSLPL"
     aSDomain        complement(5521..6354)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_vibH_Condensation_Starter.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="5.90E-59"
                     /label="vibH_Condensation_Starter.1"
                     /locus_tag="vibH"
                     /protein_end="281"
                     /protein_start="3"
                     /score="191.7"
                     /tool="antismash"
                     /translation="AQKPFWQRHLAYPHINLDTVAHSLRLTGPLDTTLLLRALHLTVSE
                     IDLFRARFSAQGELYWHPFSPPIDYQDLSIHLEAEPLAWRQIEQDLQRSSTLIDAPITS
                     HQVYRLSHSEHLIYTRAHHIVLDGYGMMLFEQRLSQHYQSLLSGQTPTAAFKPYQSYLE
                     EEAAYLTSHRYWQDKQFWQGYLREAPDLTLTSATYDPQLSHAVSLSYTLNSQLNHLLLK
                     LANANQIGWPDALVALCALYLESAEPDAPWLWLPFMNRWGSVAANVPGLMVNSLPL"
     CDS_motif       complement(5929..5994)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_vibH_0003"
                     /evalue="1.30E-09"
                     /label="C3_starter_134-155"
                     /locus_tag="vibH"
                     /protein_end="145"
                     /protein_start="123"
                     /score="30.8"
                     /tool="antismash"
                     /translation="AHHIVLDGYGMMLFEQRLSQHY"
     CDS_motif       complement(6193..6300)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_vibH_0002"
                     /evalue="3.50E-07"
                     /label="C2_starter_024-063"
                     /locus_tag="vibH"
                     /protein_end="57"
                     /protein_start="21"
                     /score="22.9"
                     /tool="antismash"
                     /translation="TVAHSLRLTGPLDTTLLLRALHLTVSEIDLFRARFS"
     CDS_motif       complement(6277..6330)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_vibH_0001"
                     /evalue="3.30E+00"
                     /label="C3_dual_135-156"
                     /locus_tag="vibH"
                     /protein_end="29"
                     /protein_start="11"
                     /score="1.1"
                     /tool="antismash"
                     /translation="HLAYPHINLDTVAHSLRL"
     protein_bind    complement(6389..6407)
                     /bound_moiety="fur"
                     /gene="vibH"
                     /note="putative"
     protein_bind    6514..6532
                     /bound_moiety="fur"
                     /gene="viuP"
                     /note="putative"
     gene            6514..7524
                     /gene="viuP"
     CDS             6547..7524
                     /codon_start=1
                     /gene="viuP"
                     /gene_functions="transport (smcogs) SMCOG1033:iron compound
                     ABC transporter, periplasmic (Score: 210.8; E-value:
                     3.6e-64)"
                     /gene_kind="transport"
                     /note="component of vibriobactin transport system"
                     /product="periplasmic binding protein"
                     /protein_id="AAD48880.1"
                     /transl_table=11
                     /translation="MRINITMKNIFILLSVILGCLFSGFVSAQQNVWPRTFQNADGSIT
                     TIPSQPKRILSTAVTVTGTLLAIDAPVIASAATTQSTFFEQWRKLAELRQVKKLWPAGS
                     VDLESVYVEQPDLIVVSMIGADSARDQIPLLQAIAPTILVDYSDQTWQSLAQQLGLATG
                     LEEQAERTIHNFEQWTKQVRDVLDLPKGRANIVSYHGPGVVNAVAKAQSAHAQLLQSVG
                     VVLEEPDPAWQAGSIVHRDFLRIHYEHLTQLQAETTFLITMTDQQAQAFLHDPILKNLP
                     SIQRKQVYGLGENSFRIDLFSAREIINSLLRRFAGEQAQSLVMP"
     gene            7521..8573
                     /gene="viuD"
     CDS             7521..8573
                     /codon_start=1
                     /gene="viuD"
                     /gene_functions="transport (smcogs) SMCOG1011:transport
                     system permease protein (Score: 302.4; E-value: 5.6e-92)"
                     /gene_kind="transport"
                     /note="component of vibriobactin transport system"
                     /product="cytoplasmic membrane permease"
                     /protein_id="AAD48881.1"
                     /transl_table=11
                     /translation="MNELMGIRVASSKRENPTLAGRDYWLMGLMLVALMGLTISSVFVG
                     SRDIPYAVTFDALFHFDEQNTQHLLVHYLRVPRTGLAILVGAALGAAGVIMQAMTRNPL
                     ADPGILGVNAGAILMVVIGIAGFGLTDMLHYLWFGLFGAAATSVGVYLLAGINQSPNPV
                     RVVLAGAAMTVVLLSMTHLIMLNSAQEVFNQFRHWSIGSLQGRGYEVLLPTSVFVLLGL
                     IAAILLARGLDTVVLGHDAGKALGVNPLWIWIASAAVVTLLAGTATAAAGPISFLGLTA
                     PHLARLWVGAEHRRLLPYSMLLGALLLLGADLAGRLIGSNDEISAGIMVALIGGPVFVL
                     LVRRWKLSHL"
     CDS             8552..9628
                     /codon_start=1
                     /gene="viuG"
                     /gene_functions="transport (smcogs) SMCOG1011:transport
                     system permease protein (Score: 274.8; E-value: 1.3e-83)"
                     /gene_kind="transport"
                     /note="component of vibriobactin transport system"
                     /product="cytoplasmic membrane permease"
                     /protein_id="AAD48882.1"
                     /transl_table=11
                     /translation="METITSVAPEGRSNQTRFWLWRKGAYTLRVERRNIAVALVLSLIV
                     LALMILATCVGKLPISISDVTAILLGEVTSGFKRQVLLDIRLPRIFTALFAGAALGLSG
                     AIFQSISRNPLGSPDIIGFTSGAATGALLQIILFSGSGVAVVFSAIAGGVMTAIAVYLL
                     SVKSGTVGRYRLVLTGIGVGSVLTALNGLLLVKGSLDSAITANLWLSGSLHAREWSHVF
                     PVMCGVLVLLPLIKSLARSLQMLEMGDEIARQLGVVVEPIRLFMILAAVTLAALATGAA
                     GPIAFIALAAPQLAARLRQTANIPLVSAGLMGACLLLGADLLTQWMPLALTLPIGRITG
                     LVGGMYLLILLMRSKNTV"
     gene            8552..9686
                     /gene="viuG"
     regulatory      9668..9686
                     /gene="viuG"
                     /note="putative rho-independent terminator"
                     /regulatory_class="terminator"
     protein_bind    9708..9726
                     /bound_moiety="fur"
                     /gene="viuC"
                     /note="putative"
     gene            9708..10584
                     /gene="viuC"
     CDS             9745..10584
                     /codon_start=1
                     /gene="viuC"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 203.3; E-value:
                     7.2e-62)"
                     /gene_kind="transport"
                     /note="component of vibriobactin transport system"
                     /product="ATPase"
                     /protein_id="AAD48883.1"
                     /transl_table=11
                     /translation="MDTIWDADLQSAALKAEHHRLKTERLSLGYEGKSVCDALDLAIPH
                     GKFSVIVGPNGCGKSTLLKSLCRLLKPLAGQVLLDGQNIHQLPTKVVAKQLGLLPQSAL
                     TPEGIKVVDLVSRGRYPHQGLFRQWSRVDERAVEEAMRATHVHELAQHSVDQLSGGQRQ
                     RVWVAMGLAQQTPYLLLDEPTTYLDIAHQIELLDLFWRLNRQQGHTLVAVLHDLNQACR
                     YADHLIVLQEGKIVAQGDPHSLVTPALVKQVFGIDCVVMPDPISCTPLIIPYSLKRG"
     gene            10587..11300
                     /gene="vibD"
     CDS             10587..11300
                     /NRPS_PKS="Domain: ACPS (112-175). E-value: 1.3e-16. Score:
                     52.5. Matches aSDomain: nrpspksdomains_vibD_ACPS.1"
                     /codon_start=1
                     /gene="vibD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1060:4'-phosphopantetheinyl transferase (Score: 192.3;
                     E-value: 1.3e-58)"
                     /gene_kind="biosynthetic-additional"
                     /product="phosphopantetheinyl transferase"
                     /protein_id="AAD48884.1"
                     /transl_table=11
                     /translation="MAFMISMPAIELAGLTIQVCQFDPQQFDEDQQHYLAPPTYHSLHK
                     AVVKRQAEFVAGRKLAQQALKQIGQGYDRPIAIGTHREPLWPAGITGSIAHCDGWAVCT
                     VLKAEHLSLGIDIEHRLAHQTASEVQAIIGTAQEWALLAQQFDLASAVTLLFSAKESLF
                     KALFPQVHLYLDFCDAQLSSLEPNKMSFTLTRVPNAAQQSCEVYFRWFEQQRVLTVSVL
                     PMLGKIEYHVPTLPT"
     misc_feature    10918..10919
                     /gene="vibD"
                     /note="site of miniTn10kan insertion in vibD"
     aSDomain        10923..11111
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_vibD_ACPS.1"
                     /evalue="1.30E-16"
                     /label="vibD_ACPS.1"
                     /locus_tag="vibD"
                     /protein_end="175"
                     /protein_start="112"
                     /score="52.5"
                     /tool="antismash"
                     /translation="LGIDIEHRLAHQTASEVQAIIGTAQEWALLAQQFDLASAVTLLFS
                     AKESLFKALFPQVHLYLD"
     CDS             11316..12179
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1125:LysR family
                     transcriptional regulator (Score: 232.7; E-value: 1e-70)"
                     /gene_kind="regulatory"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="similar to lysR family of transcription regulators"
                     /product="unknown"
                     /protein_id="AAD48885.1"
                     /transl_table=11
                     /translation="MLRDYDLNLLTVFDSVMKLGSVSKAADKLGMTSAAVSQNLSRLRE
                     QVGDPLFIRQGRGLQPTQYALNMHKHVAEGLSAIRFGLETDAGFDPATSKREFIIGGQG
                     YFDLVVLPPLLQKISDIAPHITVNLKSYEDDHFTPSQVLSEREADLFLAALPISHPSIV
                     TTQVAEEALVVVYAKNHPRLGSTLSFEQFFAEKHTALTSRRFGNYLFSSLVDQVLPARR
                     VHYQSESMLNLMATASVTDLLCFTPKRLAICGRRNWVYRSNLCRLKSARFRLLCAGIKP
                     NSKIKG"
ORIGIN
        1 ctagcccttt tttatgggcg attttcaacg gagtgaggag agggaagctc acaacgtttg
       61 gtgcttatga gcaatgagaa gcgccgccat gaagtgaaat tggattaaat gataattgtt
      121 attatttaca tccatgagtg atgttgttag catcgttgca ttattgatcg gcagaagatt
      181 cggccgaatg gctgcttctg ctgcgtcgtg cgatgacata acaacactaa caacgctcag
      241 ttggctgagt atcaataagg atgatgtact ttcatggcta ttcctaaaat cgcttcttac
      301 cctttacccg tatcattacc gactaataaa gtcgattggc gcattgatgc atcgcgggcg
      361 gtactgctta tccatgatat gcaggagtac tttgtacact attttgactc gcaggcagaa
      421 cccatcccaa gtttaatcaa gcatatacaa cagcttaaag ctcatgccaa gcaagcgggg
      481 atccctgtgg tctatacggc tcaacccgcg aatcaagatc ctgccgagcg agcactattg
      541 agcgattttt gggggcccgg tttgagtgaa gagacggcga tcattgcgcc tcttgctcca
      601 gaatcaggtg atgttcagct caccaaatgg cgttatagcg cgtttaaaaa gtcgccgtta
      661 ttggattggc tgcgtgaaac agggcgggat cagttaatca ttacgggtgt ctatgcgcac
      721 attggcattc tctcgactgc gttggatgcc tttatgttcg atattcagcc ttttgtgatt
      781 ggggatggcg tggcagattt ctcgctcagt gatcacgaat tcagtctgcg ttatatcagt
      841 ggtcgtactg gtgcggtcaa atccactcaa caagcgtgtt tagagattgc cgcccaacac
      901 tcaaagctta ctggcttaag cttgcgaacc atgcaacacg atgtggccgc tgcgctcaat
      961 ctgagtgtgg atgaagtgga tgtgcaggag aacctcctgt ttctcggttt agattcgatt
     1021 cgtgcgattc agttgttgga aaaatggaaa gcccaaggtg ctgatattag ctttgctcaa
     1081 ctgatggagc atgtcacttt gcagcagtgg tggcagacca ttcaagccaa tttacatcaa
     1141 ccttgctctg cgtgattgaa cgagttccct tcctgcttaa cacacggatt ctaagtagga
     1201 agggaaccgc ttattcaagg attccctcta ctcactcatt gtctttacat tgatttcacg
     1261 acattaacgc taagccgagc gtgtcaaatc gacgacgaag cgcgttctta tcgatttttc
     1321 caaccgatgt tttgggcagt tgatcgataa attggatttg atctgggatt ttgtaatcgg
     1381 cgagccccgc ttggtgtaaa aaacgtttga gctggatggt attcaccgac tgctcgggtt
     1441 tgagtacgat caccgcgcaa ctgcgctctc ctaggtattc gtcactgatg gcaattaaag
     1501 cggcatcatg cactgctgga tgatgtaaaa gctgattttc cacctcttcg gcggcaatct
     1561 tttcccctcc acgattgatc tgatctttgg cccttccggt gacaatcacg taaccttcgc
     1621 tactgagttt gactttatcg ccggtgatgt aaaaaccttg tgcattgaac gctcgttgat
     1681 tgtgctgatc agctcgatag taaccgcgaa tcgtataggg accttgggtt aacagatacc
     1741 cttcttctcc gctggcgaca ggttgaccct gctcatcaac caccagtaat tgatcgtgag
     1801 cggagatagg ccgaccttga gttgtagcaa tcagttccgc actgtcatcc aaacgagtgt
     1861 aattgaccaa gccttctgcc attccaaaca cttgttgcaa ttggcagcct agcgctttcg
     1921 gcagacgcct tgcggccgct tcactaaatt ttgccccgcc tacttgcaca aagtgcaatg
     1981 aggagagatc gtaggtgctt ttttctgcgt gatccatcca gagtagtgct aaaggcggca
     2041 ccaacgcggt aacagtaatt ttatgctgtt ctatcaattt aaacgcgtgt tggggtgacg
     2101 catcttgact gaggacaacg cagccacccg cccagaaaac accgagtgcg ccgggggagc
     2161 tgagagggaa gttatgtgcc gcgggcagta cgcacagata gcgagtatgc tgatcaaaac
     2221 ggcaaatttc gacactggcg gttacgctgt aggcgtaatc attgtgggta cgcgggatga
     2281 gcttgggcgt gcccgttgtg ccaccggaaa gttggaaaaa ggcgatttga ttcgggtcgg
     2341 cattcgcttg acaagatgat gccgagtagc aactcgccag ttcaataaat tttggatctg
     2401 tcactcgagt ttggccgcgt acaatcacag tctgcagtgt tgggcagcaa gcaagcagct
     2461 cttgcgccaa agcttgatag tcaaatggtc gctgagcgcc atcaatgagg taagctttgg
     2521 cttggctgtg ctgacaaaaa tagcgaattt ccgctaaacg gtgagcgggg agcgccagaa
     2581 tcggtcgtat gccttggcgc agcaaagcga aaaagcataa atagaattcc gcaacattgc
     2641 ctaattgcag cactacgtta tcgccacagc ctagcccaag ttcggtaaat ccggcagcaa
     2701 gctgctcaat acgttcgagc atggcttggt aacgccactg ctgattatca ccgaccagcg
     2761 caagcgcatt gggggtacgc tcagcacttt gctgtaagta atcgagcaag gttttatctt
     2821 gccagtagcc taactgacgg tattgagcgg caagtgcctc tggccaaggg gtaaaatcgg
     2881 ttgtcattct tatctcctta agcggtgagc acatcgttga gctcaatacc tgccgcagcc
     2941 aagatggttt tcatcttcgc gcccgtctct tccagttcac tttgtggtaa cgactcatcg
     3001 acaatccctg cgccagcaaa aagcttcatt tggtgagatc ccacttcagc acagcgaatg
     3061 gtgactaccc actcaccatt gccacgtgca tcacaccaac cgaccatgcc agtgaagtag
     3121 ccacgatcaa attcttccag ctctcttatt gcttgataag ccgattcgcg cggaaaaccg
     3181 catacggctg gagtggggtg taaatcggcg gcgacttgca aagcacaaac ctcaggatcg
     3241 ctgacttgtc cttcaagtag ggttgagagg tgcagcatgg tttcggtttc aatcacagaa
     3301 ggcaccatgg gggtgtacaa gttacgacag tagcggctca tgatccgctc gacttcttcg
     3361 acgactaaac cgtgctcatg caaatctttg gcggtattga gcagtgaggc acggcgctgc
     3421 gcattttctt gggcatcttg acttctcggg cgcgagccag ccagtgggtt tgagatgaga
     3481 tagtttccgc gtttggcgac caagagttca gggcttgcac caatcagctt tctctgctcg
     3541 tccaagctgg ccgcaaaagt atagccgtga tgattaatgg cgagcagact acgcagcaag
     3601 gtcggtagtg ctatctcttg ctcggtggca atttcaatgg cgcgcgacag caccacttta
     3661 gacagcccag agtgatgaaa catgttgagc agatggctta caccttgctt gtattgctct
     3721 cctgatggcg aggagatgag tttagccact tggcgggtga ggtgagctgg acggtttaag
     3781 cgcgggctac tcgacacgta taaggtgcgc ggaatcataa atcgagtcgg tgttttaggg
     3841 tcaaacggca ctatgccaaa cagtaatgga ttgtcgcact catcacgttt ggcctgctgc
     3901 aaaagctgtt tggcttggtt agccagttca gcaaaaggaa tggcttgctg aaaagcatga
     3961 gcgaccccac aaccgagcat tgactggttt gctgaggcaa agaaaaacgg tgagcgagtg
     4021 agttgggtgt cgatcagatg ttcagacata gtggtgtatc cgaccacttc acgcttcatg
     4081 accatgttgt ttatccatat ttgcgagtga atgggttgcg ttgccaagcg caacagatta
     4141 catttgctta cattcaaatg agaatagttt ttattaataa atgcaagcaa tttccatttg
     4201 gtctgataat caaagtgatg attgtgaagg ttgatctcgt ttgtgatagg ggatgaaaga
     4261 tatgtcgttg gttaacagca aggttcttct agtggggtca gcgcgtggga tagggttctc
     4321 cgtgcttgaa catttattgc aagcgggagc gcaagtgatg gcggcagatt gtgagtggca
     4381 gttgttgcta gagcagagtg agtcgttatt ggggcgttat ccggaccagc tcactttaaa
     4441 aaaactcgac ttagcagagc ctgaagcggt gcgcgagcaa gtcaatcaat gggctgagca
     4501 agtggcggga tttgatcatc tggtctgctg tgcaggaatc ctacatgttg cgccattgca
     4561 tgaaatgcca atggaacaag tgagttctat ttttaccgtg aatgcttttg gtgtgttggc
     4621 ctgtatgcaa ggtgtcgctt caagtatgaa agcgcgtcag cagggaagta tggtgattat
     4681 tgggtctaat gctgccaata cgccgcgcat gtcgattggg gcttatggcg cttcgaaagc
     4741 cgcattacac atgctggtta agtgcattgg tatggagcta gccccttacg gaattcgttg
     4801 taatttggtc agtcccggct ccactcgcac cgctatgcag caacagcttt ggactgagca
     4861 gtatggtgag gcacaagtca ttgccggaga tgctgcgcaa tttcggttgg ggatcccgct
     4921 caataaaatt gccgagccag cggatattgc tcaagcggtc ctgtttttgt tatccgataa
     4981 cgcaggtcac atcacgctgc atgatttacg agtggatggt ggagccacgc tcgatcattg
     5041 atcgttcatc tttcaactgg cgatagcccg tgtggaggtg gtggctgcca atgccgcagg
     5101 ctctgcgagt aggcgtgcaa catcttgctc cacctgttgg aaacgggcga gtaactgctc
     5161 gaaaaaacgt ggaaaccgct cgcaatgact ttgccagtgg gattgcgggt agctggctaa
     5221 atctgcggtg atgtctagac agagttcatg ctggggtgag ccacgaaagg taaaattaat
     5281 cccttctgca gagcctgaag ccagcacctt gagctcagtc tggcaatcag caaagtgtgg
     5341 ggattcaaaa ggcaaaatat tgataaatgg gctcataaaa taactttgct cagcatttag
     5401 cccttgatct tgttcgattt gttcaattcg atagcggcca tgcaaataga gtgagcgtat
     5461 cgcttgccct gactgtttaa ggtagttgcc gagcgaggtt tgctgcgcaa aaagacgcag
     5521 caagggcagc gagttaacca ttaaaccggg cacattagcg gccacgctgc cccatcggtt
     5581 cataaagggc agccacagcc aaggcgcatc aggttcagcc gattccaaat agagcgcgca
     5641 cagcgcgact aaggcatcag gccagccaat ctgattcgcg ttagcgagct tgagtaacaa
     5701 atggttgagt tggctattga gtgtgtaaga gaggctcacg gcatgactaa gttgaggatc
     5761 ataggttgca gaggtgagcg ttaagtcggg agcttcgcgt aaatagcctt gccaaaactg
     5821 cttatcttgc cagtagcgat ggctggtaag ataagccgcc tcttcttcca gataggattg
     5881 ataaggttta aacgcggcag ttggcgtctg cccactgagc aaggattggt aatgttggct
     5941 gaggcgctgc tcaaacagca tcatgccata accgtccagc acaatgtggt gcgcacgggt
     6001 atagataagg tgctcactgt gtgacaaacg atacacttga tggctcgtta ttggcgcgtc
     6061 aatcagtgtg cttgagcgct gtaaatcctg ctctatttgc cgccaagcta aaggttctgc
     6121 ttcaaggtgg atgctgaggt cttgatagtc gataggcgga gaaaatgggt gccaatacag
     6181 ctcaccttgc gcagaaaagc gagcacggaa cagatctatc tcactgacgg ttaaatgcaa
     6241 tgcgcgcagc aaaagtgtgg tatctaaagg ccctgtcagg cgtagcgaat gggcaacggt
     6301 gtcgagatta atgtgaggat aagccagatg gcgctgccaa aaaggctttt gagccaataa
     6361 catagacacg atattaagcc ttatccagtt ggagaatcat aattactctt tcgcgtgagg
     6421 cggtgttcgg tggcttggtt gcgagcctga gggggcatgg ctatactgag ttcacttttt
     6481 actcacccct ctgtctctag agtccaccct aagatgattg atgagcgata ttaaactttt
     6541 aacgaaatga gaatcaatat tacaatgaaa aacattttta ttttattgtc tgtgatattg
     6601 ggttgcctgt tcagtggttt tgtcagcgcg cagcagaatg tttggccaag aacctttcag
     6661 aatgcggacg gttcaatcac gacgattccc tctcagccaa agcgaatttt gtcgacggcc
     6721 gtcacggtaa ccggaacact gctggcgata gatgcgccag ttattgctag tgctgcgacc
     6781 acgcaaagca ccttttttga gcagtggcga aagctggcag agctgcgcca agtgaaaaaa
     6841 ctttggcctg cgggaagtgt cgatctcgaa tcggtgtatg tcgaacaacc tgatttgatc
     6901 gtggtttcca tgataggcgc ggattccgct cgcgatcaaa ttcccctttt acaagcgatt
     6961 gctcccacca tcttggtcga ttacagcgat caaacttggc aatctttggc gcagcagttg
     7021 gggcttgcta ccggacttga agagcaagcg gagcgcacaa tacacaattt tgagcagtgg
     7081 acaaagcaag tgcgagatgt gctggatctg cccaaagggc gcgccaatat tgtgagctac
     7141 cacggccccg gtgtggtcaa tgcggtggcg aaagcgcaaa gtgctcatgc tcaactgttg
     7201 caatcggtcg gggttgtgtt ggaggagcca gatccagcat ggcaagcggg ttccatcgtc
     7261 catcgagatt ttctgcgcat ccattatgag catttaactc agttgcaggc tgaaaccacg
     7321 tttcttatca cgatgaccga tcagcaagct caagcttttc tccacgatcc gattttgaaa
     7381 aacttgcctt cgatccagcg caaacaagtt tatggattgg gtgaaaactc atttcgcatt
     7441 gacctgttca gtgcccgaga aatcattaac tcactgctgc gacgctttgc tggcgagcaa
     7501 gcacaatctt tagtcatgcc atgaatgagc tgatgggaat aagagttgcg tcctcaaagc
     7561 gagaaaatcc gacgttggca gggcgtgatt actggctaat ggggttgatg ctggtggcat
     7621 tgatgggatt gacgataagc agtgtgtttg tcggatctcg cgacattcct tatgccgtga
     7681 ctttcgatgc gctgtttcat tttgatgagc agaatactca gcatcttcta gtgcattacc
     7741 tacgtgtgcc gcgcacgggg ttagcgatat tggtgggcgc agcgctcggg gctgcaggcg
     7801 ttatcatgca agcgatgacg cgtaacccat tggcggatcc gggcattttg ggtgtcaatg
     7861 cgggcgcaat cttgatggtg gtgattggca ttgctgggtt tggtctaacc gatatgctgc
     7921 actatttgtg gtttggctta tttggcgcgg cagcaaccag tgtgggcgtt tatctgctgg
     7981 caggtatcaa tcaatcaccc aatcccgttc gagtggtgct ggctggtgcg gcgatgacgg
     8041 tggtgctctt gtctatgact catttgatta tgctcaatag cgcccaagag gtgtttaacc
     8101 agtttcgaca ttggtcgatt ggctctttgc aaggcagagg atatgaagtt ttgctgccga
     8161 caagtgtatt cgtactactc gggttgatcg cggctatctt actcgcccgc ggattggata
     8221 ctgtggtgct cgggcatgac gcgggcaaag cattaggcgt caatccgtta tggatctgga
     8281 tagcttctgc cgccgtggtc acgctgttgg ctggcaccgc aaccgcagcg gcagggccaa
     8341 tcagttttct tggcttaacc gcgcctcatt tagcgcggct ttgggtgggc gctgaacatc
     8401 gtcgtttact gccttactct atgttgcttg gggcgctgtt attgttaggt gccgatctgg
     8461 ccgggcggct aatcggttca aacgatgaaa tcagcgcagg gatcatggtc gccttaatcg
     8521 gagggccagt ctttgtgcta ttggtgagac gatggaaact atcacatctg tagccccaga
     8581 aggtcggtcg aatcaaactc gcttttggct atggcgaaaa ggggcataca cgctgcgtgt
     8641 tgagcggcgc aacattgcgg ttgcgttggt gctaagcctt attgttttgg ctttgatgat
     8701 acttgcgacc tgtgtcggca aattgccgat ttcaatctct gacgtcactg ccattttatt
     8761 gggggaagtt acctcagggt tcaaacggca agtcttactc gatatccgtt taccacggat
     8821 ttttacggcg ctgtttgctg gtgcggcctt aggcctttcc ggcgcgattt ttcagtcgat
     8881 ctctcgtaat ccgcttggtt ctccggacat cattggtttc acctctggcg cggccaccgg
     8941 ggctttgctg caaattattt tattttccgg cagtggggtt gccgtggttt tttcggcgat
     9001 tgctggcgga gtcatgacgg ccatcgcagt ctatctgttg tcggtaaagt caggcacggt
     9061 cgggcgctat cggctagtgc taaccggaat tggcgtgggt tcagtgttga cggcgctcaa
     9121 tggcttgctg ctagtcaaag gcagtttaga cagcgccatc accgccaatt tatggttatc
     9181 gggctcactg catgcccgag aatggagcca cgtatttcct gtcatgtgtg gcgtgttggt
     9241 gctgctgccg cttatcaaaa gtcttgcccg ctcattacag atgcttgaga tgggggatga
     9301 gattgcccgt caacttgggg tcgtagtcga gccgatacgc ttattcatga ttctcgccgc
     9361 ggtgactttg gcggcattag cgacaggagc cgcaggaccg atcgccttta tcgctttggc
     9421 cgctcctcaa cttgcagctc gcttgcgtca aaccgccaat attccgctgg tgagtgcagg
     9481 tttaatggga gcttgcttac tgttgggggc tgatttgctc actcagtgga tgccgctcgc
     9541 cttaaccctt cccatcgggc gaattacggg attggttggg ggcatgtatt tattaatctt
     9601 attgatgcgt tcaaaaaata cggtttaagc cgcatcacat gccgacaggt tttttaggct
     9661 atttgcgcgc agtcgtcagc ctgtgctttg agccattcaa cgctttgcgg aataaagaga
     9721 attttgattg agtgaaatgc cgttatggac acaatttggg atgctgactt acagagcgct
     9781 gcattaaagg ctgaacatca caggcttaag actgagcgac tgagtttagg ttatgaagga
     9841 aaatcggtgt gtgatgcgct ggatttggcg attccgcacg ggaaattttc ggtgatagtt
     9901 gggcctaatg gctgtggtaa atcgaccttg ctaaaaagct tgtgccgttt actcaagccg
     9961 ctggctgggc aagtattact ggatggtcag aatattcacc aattgccgac caaggtggta
    10021 gccaagcagc taggattact gcctcaaagc gcgttgacac ccgaagggat aaaggtggtc
    10081 gatttggtct ctcgtggccg ttatcctcac caagggctat ttcgccaatg gagcagggtg
    10141 gatgaacgag cggtagaaga ggcgatgcga gccactcacg tccatgagct tgcgcagcat
    10201 agtgtggatc agctttccgg cggtcagcgt cagcgggttt gggtcgccat gggcctggcg
    10261 cagcaaacgc cgtatttact gcttgatgaa ccaaccactt atcttgatat tgctcaccaa
    10321 atcgaactgc tcgatctctt ttggcggctt aatcgtcagc aagggcatac tctagtcgcc
    10381 gtgctgcacg atctgaatca ggcgtgtcgt tacgccgacc atctgattgt gttgcaagaa
    10441 ggcaaaatcg tcgcgcaggg cgatccacac tctttggtga ctccagcatt ggttaagcaa
    10501 gtctttggga ttgattgtgt cgttatgccc gatcctatct cctgcacgcc tttgattata
    10561 ccttattcgt taaagcgcgg ttaaacatgg cttttatgat ttcaatgcca gcgattgagc
    10621 tggcagggct gacaattcag gtttgccaat tcgatcctca gcaatttgat gaggatcagc
    10681 agcactattt agcccctccc acctaccata gcctgcacaa ggccgtggtt aagcgccaag
    10741 cggaatttgt cgcaggacgt aagttagcgc agcaagcgct caaacagatc gggcagggtt
    10801 atgataggcc aattgctatc ggaacccatc gtgagccact atggcctgcg gggatcacgg
    10861 gctcgatcgc ccattgtgac ggctgggcag tatgcactgt gcttaaggca gaacacctct
    10921 cgcttggtat tgatattgaa catcggctcg cacatcaaac cgctagtgag gtgcaagcta
    10981 taatcggcac cgcccaagag tgggcactgt tggcgcaaca gttcgatctc gcttcagcgg
    11041 tgactttgct gttttctgca aaagagagtc tatttaaagc actattccca caagtacacc
    11101 tctaccttga tttctgcgat gcccagctca gttcactgga gccaaacaaa atgagtttca
    11161 cattgacgcg cgtacctaat gccgcacaac agagttgtga ggtttacttt cgttggtttg
    11221 aacaacagcg agtattgacc gtcagcgtgt taccaatgct aggcaaaatc gagtatcatg
    11281 tccccacctt acctacttga gttaacttat tgattatgct gcgtgattat gatctaaacc
    11341 tgctgacggt atttgattct gtcatgaaat taggctcagt cagtaaagca gcggataagc
    11401 taggtatgac ttccgcagcg gtgagtcaaa atttatcacg cttacgagaa caagttggcg
    11461 atccgttgtt tatccgtcag ggacgtgggt tacagccgac gcagtatgcg ctcaatatgc
    11521 acaaacatgt ggcggaaggc ttgagtgcca ttcgttttgg tttagaaacc gatgccggtt
    11581 ttgatcctgc aaccagtaag cgtgagttta ttattggcgg tcagggctat ttcgatttgg
    11641 tggtattacc gccacttttg caaaaaatca gcgacatcgc cccgcatatc acggtgaatt
    11701 taaagtctta tgaggacgat catttcacac caagccaagt attaagtgag cgagaggcgg
    11761 atctgttttt ggctgcatta cccatcagcc atccctctat cgtcaccaca caggtggcgg
    11821 aggaggcctt ggttgtggtg tatgccaaaa atcatccgcg tcttggaagt accctcagtt
    11881 ttgagcagtt ttttgcagaa aaacacactg cattaaccag tcgtcgcttt ggtaattatc
    11941 tcttttctag cctagtcgat caggtgctgc ccgcacgtcg agtgcattat caaagtgagt
    12001 cgatgctcaa tttgatggcg accgcatccg tcaccgatct gctctgtttt actccaaagc
    12061 gtttagcgat atgtgggcgg agaaattggg tttacagatc caacctttgc cgtttgaaat
    12121 ccgctcggtt ccgactttta tgtgctggca taaagccaaa cagcaagata aagggctgat
    12181 ttggctgcgc gagcaaattg
//
