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Overview
1.1

### Identified secondary metabolite regions using strictness 'relaxed'

BGC0002497 (Streptomyces sp)

Region | Type | From | To | Similarity Confidence | Most similar known cluster
--- | --- | --- | --- | --- | ---
Region 1 | T1PKS,hglE-KS | 1 | 131,383 | High | hexacosalactone A | other:other

BGC0002497 - Region 1 - T1PKS,hglE-KS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1 - 131,383 nt. (total: 131,383 nt) Show pHMM detection rules used
Region on contig edge.
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
hglE-KS: (hglE or hglD)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
MIBiG
Tailoring
MIBiG comparison
KnownClusterBlast
NRPS/PKS modules
NRPS/PKS domains
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
External annotation
This annotation was made externally by 'MIBiG' and not by antiSMASH

Name |  | MIBiG
Version |  | 4.0alpha

Type |  | Subregion
Location |  | 1 - 131,384
Label |  | BGC0002497

Tailoring functions
Tailoring enzymes detected in this region
Oxidoreductases: dehydrogenase oxidoreductase oxygenase
hcl15: aldo/keto reductase family oxidoreductase

smcogs
  SMCOG1039: aldo/keto reductase family oxidoreductase

hcl27: short-chain dehydrogenase/reductase SDR

smcogs
  SMCOG1001: short-chain dehydrogenase/reductase SDR

hcl30: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

hcl8: aldehyde dehydrogenase

smcogs
  SMCOG1017: aldehyde dehydrogenase

Transferases: acylase glycosyltransferase methyltransferase
hcl12: malonyl CoA-acyl carrier protein transacylase

smcogs
  SMCOG1021: malonyl CoA-acyl carrier protein transacylase

hcl14: methyltransferase

smcogs
  SMCOG1089: methyltransferase

hcl23: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

Hydrolases: hydrolase
hcl22: alpha/beta hydrolase fold protein

smcogs
  SMCOG1036: alpha/beta hydrolase fold protein

Ligases: ligase synthase
hcl19: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

hcl20: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

hcl3_rename1: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

hclA: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

hclB: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

hclD: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

hclX: AMP-dependent synthetase and ligase

smcogs
  SMCOG1002: AMP-dependent synthetase and ligase

hclY: AMP-dependent synthetase and ligase

smcogs
  SMCOG1002: AMP-dependent synthetase and ligase

hclZ: 8-amino-7-oxononanoate synthase

smcogs
  SMCOG1109: 8-amino-7-oxononanoate synthase

Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | T1PKS | hglE-KS | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | --- | ---
BGC0002497.3 |  |  | 1.29 | other (other) | hexacosalactone A | Streptomyces sp.
BGC0001276.3 |  |  | 0.75 | PKS | 6-methylsalicyclic acid | Aspergillus terreus
BGC0001273.3 |  |  | 0.74 | PKS | asperlactone | Aspergillus ochraceus
BGC0001244.3 |  |  | 0.73 | PKS | (-)-Mellein | Parastagonospora nodorum
BGC0001275.3 |  |  | 0.73 | PKS | 6-methylsalicyclic acid | Glarea lozoyensis
BGC0000041.3 |  |  | 0.66 | PKS | oronofacic acid | Pseudomonas syringae
BGC0001160.5 |  |  | 0.60 | PKS | 1-nonadecene, (14Z)-1,14-nonadecadiene | Synechococcus sp. PCC 7002
BGC0001164.3 |  |  | 0.59 | PKS | 1-heptadecene | Cyanothece sp. PCC 7822
BGC0002192.3 |  |  | 0.58 | PKS | FR901512 | fungal sp. No.14919
BGC0001254.3 |  |  | 0.56 | PKS | ACT-Toxin II | Alternaria alternata

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0002497.3 |  | 0.99 | other (other) | hexacosalactone A | Streptomyces sp.
BGC0001276.3 |  | 0.76 | PKS | 6-methylsalicyclic acid | Aspergillus terreus
BGC0001244.3 |  | 0.75 | PKS | (-)-Mellein | Parastagonospora nodorum
BGC0001273.3 |  | 0.75 | PKS | asperlactone | Aspergillus ochraceus
BGC0001275.3 |  | 0.75 | PKS | 6-methylsalicyclic acid | Glarea lozoyensis
BGC0000041.3 |  | 0.72 | PKS | oronofacic acid | Pseudomonas syringae
BGC0000067.5 |  | 0.66 | PKS | geldanamycin | Streptomyces hygroscopicus subsp. duamyceticus
BGC0001160.5 |  | 0.64 | PKS | 1-nonadecene, (14Z)-1,14-nonadecadiene | Synechococcus sp. PCC 7002
BGC0001164.3 |  | 0.64 | PKS | 1-heptadecene | Cyanothece sp. PCC 7822
BGC0002192.3 |  | 0.64 | PKS | FR901512 | fungal sp. No.14919

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.
Location:
Identifier
Identity
Coverage
Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
NRPS/PKS products
NRPS/PKS substrates
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 1, location 213 - 124554:
Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:
  (pk - ohmal - pk - pk) + (Me-ohmal - ohmal - pk - ohmal) + (pk - ccmal) + (mal) + (Me-mal) + (Me-ccmal - ccmal - pk)
Non-colinear ordering:
  hclA + hclB + hcl3_rename1 + hcl19 + hcl20 + hcl21 + hclD

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).
hclX: X
AMP-binding (9..412): X
nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:
  Predicted physicochemical class:
    hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
  Large clusters prediction:
    N/A
  Small clusters prediction:
    N/A
  Single AA prediction:
    N/A

Stachelhaus prediction details:
  Stachelhaus sequence:
    TFTMFGGPVK
  Nearest Stachelhaus code(s):
    Leu DFLYFGGVVK (29% 8Å match)
  Stachelhaus code match:
    60% (weak)

hclY: X
AMP-binding (15..416): X
nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:
  Predicted physicochemical class:
    hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
  Large clusters prediction:
    Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
  Small clusters prediction:
    N/A
  Single AA prediction:
    N/A

Stachelhaus prediction details:
  Stachelhaus sequence:
    SGATSGFGIK
  Nearest Stachelhaus code(s):
  Stachelhaus code match:
    0% (weak)

hcl12: pk
PKS_AT (12..278): pk
ATSignature: (unknown)
No matches above 50%
minowa: 2-Methylbutyryl-CoA

Prediction, score:
  2-Methylbutyryl-CoA: 7.5
  trans-1,2-CPDA: 0.0
  inactive: 0.0
  fatty_acid: 0.0
  Propionyl-CoA: 0.0
  Methylmalonyl-CoA: 0.0
  Methoxymalonyl-CoA: 0.0
  Malonyl-CoA: 0.0
  Isobutyryl-CoA: 0.0
  Ethylmalonyl-CoA: 0.0
  CHC-CoA: 0.0
  Benzoyl-CoA: 0.0
  Acetyl-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0

hclA: pk - mal - pk - pk
PKS_AT (560..858): pk
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 87.5%
  Malonyl-CoA: 83.3%
  Methoxymalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 210.7
  Methylmalonyl-CoA: 205.8
  2-Methylbutyryl-CoA: 185.4
  Methoxymalonyl-CoA: 168.1
  trans-1,2-CPDA: 134.1
  Propionyl-CoA: 128.3
  CHC-CoA: 114.6
  Malonyl-CoA: 94.9
  Isobutyryl-CoA: 91.4
  Benzoyl-CoA: 67.6
  Acetyl-CoA: 51.7
  3-Methylbutyryl-CoA: 43.7
  fatty_acid: 34.6
  inactive: 24.5

PKS_AT (1585..1870): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 233.1
  inactive: 121.2
  Methoxymalonyl-CoA: 56.4
  Methylmalonyl-CoA: 46.5
  Propionyl-CoA: 34.7
  2-Methylbutyryl-CoA: 16.3
  Isobutyryl-CoA: 15.7
  Acetyl-CoA: 11.1
  Benzoyl-CoA: 9.3
  Ethylmalonyl-CoA: 7.4
  trans-1,2-CPDA: 0.0
  fatty_acid: 0.0
  CHC-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0

PKS_AT (3070..3368): pk
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 231.8
  Methylmalonyl-CoA: 225.7
  Methoxymalonyl-CoA: 182.8
  2-Methylbutyryl-CoA: 167.8
  trans-1,2-CPDA: 134.5
  Propionyl-CoA: 129.3
  CHC-CoA: 108.9
  Malonyl-CoA: 108.5
  Isobutyryl-CoA: 73.9
  Benzoyl-CoA: 62.8
  Acetyl-CoA: 38.0
  fatty_acid: 36.5
  inactive: 24.1
  3-Methylbutyryl-CoA: 21.8

PKS_AT (4404..4698): pk
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 229.1
  Methylmalonyl-CoA: 224.9
  Methoxymalonyl-CoA: 180.2
  2-Methylbutyryl-CoA: 160.8
  trans-1,2-CPDA: 128.6
  Propionyl-CoA: 118.4
  CHC-CoA: 113.9
  Malonyl-CoA: 104.0
  Isobutyryl-CoA: 80.1
  Benzoyl-CoA: 67.0
  Acetyl-CoA: 40.0
  fatty_acid: 38.3
  3-Methylbutyryl-CoA: 32.4
  inactive: 27.4

hclB: mmal - mal - pk - mal
PKS_AT (576..874): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 225.9
  Ethylmalonyl-CoA: 224.0
  Methoxymalonyl-CoA: 181.5
  2-Methylbutyryl-CoA: 181.3
  Propionyl-CoA: 133.7
  trans-1,2-CPDA: 129.8
  CHC-CoA: 115.2
  Malonyl-CoA: 103.6
  Isobutyryl-CoA: 84.3
  Benzoyl-CoA: 65.0
  fatty_acid: 41.2
  Acetyl-CoA: 39.9
  3-Methylbutyryl-CoA: 36.3
  inactive: 27.0

PKS_AT (2150..2441): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 100.0%
  inactive: 79.2%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 236.0
  inactive: 118.6
  Methoxymalonyl-CoA: 56.3
  Methylmalonyl-CoA: 44.6
  Propionyl-CoA: 31.4
  Isobutyryl-CoA: 20.2
  2-Methylbutyryl-CoA: 14.9
  Benzoyl-CoA: 12.7
  Ethylmalonyl-CoA: 11.0
  Acetyl-CoA: 10.1
  fatty_acid: 7.2
  trans-1,2-CPDA: 0.0
  CHC-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0

PKS_AT (3708..4006): pk
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 233.5
  Methylmalonyl-CoA: 232.1
  Methoxymalonyl-CoA: 187.6
  2-Methylbutyryl-CoA: 169.3
  trans-1,2-CPDA: 149.9
  Propionyl-CoA: 130.3
  Malonyl-CoA: 112.5
  CHC-CoA: 112.2
  Isobutyryl-CoA: 78.8
  Benzoyl-CoA: 71.1
  Acetyl-CoA: 45.0
  fatty_acid: 37.4
  3-Methylbutyryl-CoA: 27.5
  inactive: 25.5

PKS_AT (5498..5787): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 229.1
  inactive: 121.6
  Methoxymalonyl-CoA: 59.6
  Methylmalonyl-CoA: 47.9
  Propionyl-CoA: 33.8
  2-Methylbutyryl-CoA: 15.5
  Isobutyryl-CoA: 15.0
  Acetyl-CoA: 12.1
  Benzoyl-CoA: 9.3
  Ethylmalonyl-CoA: 3.9
  trans-1,2-CPDA: 0.0
  fatty_acid: 0.0
  CHC-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0

hcl3_rename1: pk - mal
PKS_AT (583..877): pk
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 230.0
  Methylmalonyl-CoA: 228.0
  Methoxymalonyl-CoA: 181.5
  2-Methylbutyryl-CoA: 159.2
  trans-1,2-CPDA: 134.3
  Propionyl-CoA: 118.4
  CHC-CoA: 117.7
  Malonyl-CoA: 99.5
  Isobutyryl-CoA: 86.4
  Benzoyl-CoA: 71.4
  Acetyl-CoA: 38.1
  3-Methylbutyryl-CoA: 35.6
  fatty_acid: 31.6
  inactive: 24.3

PKS_AT (2346..2634): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 225.7
  inactive: 115.4
  Methoxymalonyl-CoA: 52.1
  Methylmalonyl-CoA: 44.1
  Propionyl-CoA: 32.4
  Isobutyryl-CoA: 21.9
  Acetyl-CoA: 13.1
  Benzoyl-CoA: 12.3
  2-Methylbutyryl-CoA: 7.6
  trans-1,2-CPDA: 0.0
  fatty_acid: 0.0
  Ethylmalonyl-CoA: 0.0
  CHC-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0

hclD: mmal - mal - pk
PKS_AT (570..868): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 219.3
  Ethylmalonyl-CoA: 217.3
  Methoxymalonyl-CoA: 175.2
  2-Methylbutyryl-CoA: 148.7
  trans-1,2-CPDA: 131.6
  CHC-CoA: 105.7
  Propionyl-CoA: 104.0
  Malonyl-CoA: 85.4
  Isobutyryl-CoA: 77.2
  Benzoyl-CoA: 60.9
  Acetyl-CoA: 35.1
  3-Methylbutyryl-CoA: 34.0
  fatty_acid: 28.7
  inactive: 22.3

PKS_AT (2351..2638): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 232.0
  inactive: 114.8
  Methoxymalonyl-CoA: 63.4
  Methylmalonyl-CoA: 54.9
  Propionyl-CoA: 39.0
  2-Methylbutyryl-CoA: 21.0
  Isobutyryl-CoA: 16.6
  Acetyl-CoA: 13.5
  Benzoyl-CoA: 12.5
  Ethylmalonyl-CoA: 11.4
  fatty_acid: 8.4
  3-Methylbutyryl-CoA: 6.9
  trans-1,2-CPDA: 0.0
  CHC-CoA: 0.0

PKS_AT (4205..4503): pk
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 235.0
  Methylmalonyl-CoA: 229.2
  Methoxymalonyl-CoA: 186.5
  2-Methylbutyryl-CoA: 161.6
  trans-1,2-CPDA: 138.5
  Propionyl-CoA: 124.2
  CHC-CoA: 114.3
  Malonyl-CoA: 107.7
  Isobutyryl-CoA: 77.6
  Benzoyl-CoA: 72.0
  Acetyl-CoA: 47.9
  fatty_acid: 33.5
  3-Methylbutyryl-CoA: 31.4
  inactive: 30.7

hcl19: mal
PKS_AT (1274..1566): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 79.2%
  inactive: 58.3%
  Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 73.6
  Methoxymalonyl-CoA: 35.1
  Methylmalonyl-CoA: 32.7
  inactive: 24.4
  Propionyl-CoA: 18.8
  CHC-CoA: 10.2
  Isobutyryl-CoA: 7.6
  trans-1,2-CPDA: 0.0
  fatty_acid: 0.0
  Ethylmalonyl-CoA: 0.0
  Benzoyl-CoA: 0.0
  Acetyl-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0
  2-Methylbutyryl-CoA: 0.0

hcl20: mmal
PKS_AT (631..843): mmal
ATSignature: (unknown)
No matches above 50%
minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 8.5
  Malonyl-CoA: 4.6
  trans-1,2-CPDA: 0.0
  inactive: 0.0
  fatty_acid: 0.0
  Propionyl-CoA: 0.0
  Methoxymalonyl-CoA: 0.0
  Isobutyryl-CoA: 0.0
  Ethylmalonyl-CoA: 0.0
  CHC-CoA: 0.0
  Benzoyl-CoA: 0.0
  Acetyl-CoA: 0.0
  3-Methylbutyryl-CoA: 0.0
  2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor