LOCUS       BGC0002510             11781 bp    DNA     linear   BCT 05-SEP-2020
DEFINITION  Streptomyces sp. strain OUCT-12 sap gene cluster, complete sequence.
ACCESSION   BGC0002510
VERSION     BGC0002510
KEYWORDS    .
SOURCE      Streptomyces sp
  ORGANISM  Streptomyces sp.
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 10:07:44
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 70
            Orig. end    :: 11851
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..258
                     /gene="sapA"
                     /note="acyl carrier protein"
     CDS             1..258
                     /NRPS_PKS="Domain: PP-binding (9-75). E-value: 1.6e-16.
                     Score: 52.5. Matches aSDomain:
                     nrpspksdomains_sapA_PP-binding.1"
                     /codon_start=1
                     /gene="sapA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /note="acyl carrier protein"
                     /product="SapA"
                     /protein_id="QMX85609.1"
                     /sec_met_domain="PP-binding (E-value: 4.1e-17, bitscore:
                     52.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEAREQHLDELREIVAEVLEVEPEEITETGSFAEEHEADSLRAI
                     EILARIEKKYKIDIPQSELPQMENLKAVYDVVAQQAGWQD"
     misc_feature    1..11781
                     /note="sap gene cluster"
     subregion       1..11781
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="Polyketide"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11781
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     aSDomain        28..225
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_sapA_PP-binding.1"
                     /evalue="1.60E-16"
                     /label="sapA_PP-binding.1"
                     /locus_tag="sapA"
                     /protein_end="75"
                     /protein_start="9"
                     /score="52.5"
                     /tool="antismash"
                     /translation="DELREIVAEVLEVEPEEITETGSFAEEHEADSLRAIEILARIEKK
                     YKIDIPQSELPQMENLKAVYD"
     gene            385..843
                     /gene="sapB"
     CDS             385..843
                     /codon_start=1
                     /gene="sapB"
                     /note="DUF3995"
                     /product="SapB"
                     /protein_id="QMX85611.1"
                     /transl_table=11
                     /translation="MAVVFGLFSFYWAAGGTFGLDTLGGAIQRMAERRDSGFIAVVWIT
                     GALKVAGGLFSLSLVRPWSRVVPRPLMLLAGWGGAALLTVYGALQVGSLSLVAFGVLTP
                     SDPVEWKPLLWRLFVWDMSFLVWGILLFLATRTYVMNHPRRPERRGGN"
     gene            843..1847
                     /gene="sapC"
     CDS             843..1847
                     /codon_start=1
                     /gene="sapC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081:cysteine synthase (Score: 367.3; E-value:
                     8.5e-112)"
                     /gene_kind="biosynthetic-additional"
                     /note="cysteine_synthase"
                     /product="SapC"
                     /protein_id="QMX85610.1"
                     /transl_table=11
                     /translation="MYDSAQDLVFDRVFLRIDGLVPEIRLLLKIEGFNPAGSIKLKTAI
                     GLVEDAERWNLLRPGGHIIESSSGNLGVALASVCAARGYRFTCVTDPNTSPQNAALIRA
                     FGATVVVVDTKDANGGYLQSRIDYIHDRLREDPSLFWPNQYANEANPRIHRDRTAAAIL
                     DEVPEVDHLFIGAGTTGTLMGCAAHFRERSPDTRIVAVDTAGSVTFGFPPGKRHLPGLG
                     TSRKPELCDPRLVDDIVIVPEAEAVRMCRTIARSRGIATGASTGSVLAAVVRRGPELPP
                     GSTVVAIAPDTGDRYLNTVYNDTWVEERFGHQALIPAGADDHPDADGSLLLKG"
     gene            1849..2856
                     /gene="sapD"
     CDS             1849..2856
                     /codon_start=1
                     /gene="sapD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158:ornithine cyclodeaminase (Score: 387; E-value:
                     8.5e-118)"
                     /gene_kind="biosynthetic-additional"
                     /note="ornithine_cyclodeaminase"
                     /product="SapD"
                     /protein_id="QMX85608.1"
                     /transl_table=11
                     /translation="MFDFHVVSGETARRVIGRSRSHIVDQVRDTYLTHDLGDSVNPNSY
                     FLRFPDKPDSRIIALPAYLGGEHSVAGIKWIASFPANIERSVPRASAVLLLNDHETGYP
                     FACLEAAQISAARTAASAVLAAETLHGSRTARRLLVIGAGIIARNILEFFTDRDWQLDE
                     VLVHDRDPRYAESLTEHVSGALGHRARVAPDRDKAIAEADLVVLATTAAEPHITRPGTF
                     APGQIVLNISLRDIDPEIIVGAHNILDDIDHCLTANTSPHLAEQKFGHREFINGTLADV
                     IRGRRKLATDRPVIFSPFGLGVLDLAVGAFVHRAALEENEATAIEGFFGETTRW"
     gene            2949..3689
                     /gene="sapE"
     CDS             2949..3689
                     /NRPS_PKS="Domain: PKS_KR (7-167). E-value: 4e-24. Score:
                     77.3. Matches aSDomain: nrpspksdomains_sapE_PKS_KR.1"
                     /codon_start=1
                     /gene="sapE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     251; E-value: 1.9e-76)"
                     /gene_kind="biosynthetic-additional"
                     /note="short-chain_dehydrogenase/reductase_SDR"
                     /product="SapE"
                     /protein_id="QMX85612.1"
                     /sec_met_domain="adh_short (E-value: 2.1e-45, bitscore:
                     144.7, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 4.9e-38, bitscore:
                     121.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTQRRALVTGGSRGIGASVALRLAQDGMDVAFCYRSEKERAEET
                     AERIRELGVRCFHAPCDVADFEAVQSFIKEAEADLGAIDTLVNSAGIVRDNPLVLMPVE
                     DWDAVIDASLTGVFHFCRSMAFGFMKRKRGVIVNVSSVAGVYGNKAQTNYSAAKAGING
                     MSKALAKELAPYGLRVNVVAPGFIETDMTAGLTDKVRAQALELVPLRRFGTPEDVAELV
                     SFLVSDRAGYITGQILQVDGGIVL"
     CDS_motif       2970..3032
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_sapE_0001"
                     /evalue="2.10E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="sapE"
                     /protein_end="28"
                     /protein_start="7"
                     /score="17.2"
                     /tool="antismash"
                     /translation="LVTGGSRGIGASVALRLAQDG"
     aSDomain        2970..3449
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_sapE_PKS_KR.1"
                     /evalue="4.00E-24"
                     /label="sapE_PKS_KR.1"
                     /locus_tag="sapE"
                     /protein_end="167"
                     /protein_start="7"
                     /score="77.3"
                     /tool="antismash"
                     /translation="LVTGGSRGIGASVALRLAQDGMDVAFCYRSEKERAEETAERIREL
                     GVRCFHAPCDVADFEAVQSFIKEAEADLGAIDTLVNSAGIVRDNPLVLMPVEDWDAVID
                     ASLTGVFHFCRSMAFGFMKRKRGVIVNVSSVAGVYGNKAQTNYSAAKAGINGMSKA"
     gene            3686..4120
                     /gene="sapF"
     CDS             3686..4120
                     /codon_start=1
                     /gene="sapF"
                     /note="dehydratase"
                     /product="SapF"
                     /protein_id="QMX85613.1"
                     /transl_table=11
                     /translation="MSRRFAAPLTAVDTVDIADTPAGTRVEAVKRITGDEPYLVGHYPD
                     FTIYPGVFTLESVHQAIRHWATERHGPGALAELATITSISFAAPLLPGDTLRIGCELTE
                     RDDAPAAVRAKVRCHRGDGALSARMTLELRIDRRDGDDHA"
     gene            4107..4589
                     /gene="sapG"
     CDS             4107..4589
                     /codon_start=1
                     /gene="sapG"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1270:UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine
                     (Score: 66.7; E-value: 3.4e-20)"
                     /gene_kind="biosynthetic-additional"
                     /note="dehydratase"
                     /product="SapG"
                     /protein_id="QMX85614.1"
                     /transl_table=11
                     /translation="MTTLEHADIRRILPHRHPVLLVDRVLDLEPGVRIVATKAVTGGEP
                     CYAGLDEDLPVSAYAYPASLLVESFGQTGAVLWLYSAATSGERREGTLIFGSAREVTIT
                     GSAYPGDVLRHTVEIDQLIGDNAFMRGETWVGDRRIATLGSVLAVVREGLAAGTGS"
     gene            4731..5939
                     /gene="sapH"
     CDS             4731..5939
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (34-399). E-value:
                     1.3e-56. Score: 184.1. Matches aSDomain:
                     nrpspksdomains_sapH_PKS_KS.1"
                     /codon_start=1
                     /gene="sapH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ladderane: ladderane"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 332; E-value:
                     9.2e-101)"
                     /gene_kind="biosynthetic"
                     /note="Beta-ketoacyl_synthase"
                     /product="SapH"
                     /protein_id="QMX85615.1"
                     /sec_met_domain="ladderane (E-value: 4.4e-106, bitscore:
                     345.0, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRRVVITGLGPVSSIGTGVTAFGRALRAGTSGISAISSFDSSGF
                     PHYMAGEVPDFDPKALLRTLSTAEWGRTSLFAAAAARLAVADAGIDEKVLAGAAAGSSI
                     GTTSGESQVIEALTAEWVHTGLAEVTPAYAAQVPASRLATAVNQELGLTGEAVTLSTAC
                     SASNYALGYAYDQIVTGDADYMIAGGADSVCRWAHAGFYRLGALTEKVCSPFDKDRSGI
                     LTGEGGAALFLETLESATGRGAHIYAEVLGYGLNCDANHMVAPDPVSIAECMRRAHHNA
                     GIAPGDVDYICAHGTGTPANDAMEARAVVEVFGTRPPPISSIKSMLGHTMGAASGFGAI
                     AAALAIDTGFLPPTTNYRTPDPALPAIDPVPNESRPARPEVVQNNGFAFGGNNAITILG
                     RVA"
     aSDomain        4833..5927
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_sapH_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="1.30E-56"
                     /label="sapH_PKS_KS.1"
                     /locus_tag="sapH"
                     /protein_end="399"
                     /protein_start="34"
                     /score="184.1"
                     /tool="antismash"
                     /translation="SAISSFDSSGFPHYMAGEVPDFDPKALLRTLSTAEWGRTSLFAAA
                     AARLAVADAGIDEKVLAGAAAGSSIGTTSGESQVIEALTAEWVHTGLAEVTPAYAAQVP
                     ASRLATAVNQELGLTGEAVTLSTACSASNYALGYAYDQIVTGDADYMIAGGADSVCRWA
                     HAGFYRLGALTEKVCSPFDKDRSGILTGEGGAALFLETLESATGRGAHIYAEVLGYGLN
                     CDANHMVAPDPVSIAECMRRAHHNAGIAPGDVDYICAHGTGTPANDAMEARAVVEVFGT
                     RPPPISSIKSMLGHTMGAASGFGAIAAALAIDTGFLPPTTNYRTPDPALPAIDPVPNES
                     RPARPEVVQNNGFAFGGNNAITILG"
     CDS_motif       5190..5237
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_sapH_0001"
                     /evalue="7.50E-03"
                     /label="PKSI-KS_m3"
                     /locus_tag="sapH"
                     /protein_end="169"
                     /protein_start="153"
                     /score="9.2"
                     /tool="antismash"
                     /translation="GEAVTLSTACSASNYA"
     gene            5936..7114
                     /gene="sapI"
     CDS             5936..7114
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (99-352). E-value:
                     1.4e-09. Score: 29.1. Matches aSDomain:
                     nrpspksdomains_sapI_PKS_KS.1"
                     /codon_start=1
                     /gene="sapI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) t2fas"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 111.8; E-value:
                     5.4e-34)"
                     /gene_kind="biosynthetic-additional"
                     /note="Beta-ketoacyl_synthase"
                     /product="SapI"
                     /protein_id="QMX85616.1"
                     /sec_met_domain="t2fas (E-value: 2.6e-37, bitscore: 118.4,
                     seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNATATSPDLTGHGDVRGNTLAITGWGVVTSLAIGAEEFTTAIGE
                     GRSGLRDVTGMFDEELPENQAFAMPDFTVRDHLGRKGTSFFDRSTSLGLLGCTLALDDT
                     DLTVTDDNRDRFGIVLGTTAGSIKATSDYSRETLEGDRPFLVNPVLFPNTVMNCAAGQS
                     AIWLGLKGVNATVAGGRLAGLSALRYARNQIRRGYADALLVGSVEEFSPHTAWFSHRQH
                     SAEEDRVTVGEGSAVFVVENAEAVRAQGRTPDAEVLAVEVGTYGAPGREPDAGAGLADR
                     IGRALRRAGVEPSQVRAVATGESGIARLDDIERRGIESALGAEVRRVRAKEWVGECQSA
                     SGSLQIAALLAGHRGHPELDGAVSVVTSVSPDGGVGAAVLRGWHRARGDHRS"
     aSDomain        6233..6991
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_sapI_PKS_KS.1"
                     /domain_subtype="Modular-KS"
                     /evalue="1.40E-09"
                     /label="sapI_PKS_KS.1"
                     /locus_tag="sapI"
                     /protein_end="352"
                     /protein_start="99"
                     /score="29.1"
                     /tool="antismash"
                     /translation="ALDDTDLTVTDDNRDRFGIVLGTTAGSIKATSDYSRETLEGDRPF
                     LVNPVLFPNTVMNCAAGQSAIWLGLKGVNATVAGGRLAGLSALRYARNQIRRGYADALL
                     VGSVEEFSPHTAWFSHRQHSAEEDRVTVGEGSAVFVVENAEAVRAQGRTPDAEVLAVEV
                     GTYGAPGREPDAGAGLADRIGRALRRAGVEPSQVRAVATGESGIARLDDIERRGIESAL
                     GAEVRRVRAKEWVGECQSASGSLQIAALLAG"
     gene            7089..8225
                     /gene="sapJ"
     CDS             7089..8225
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (131-374). E-value:
                     7.6e-39. Score: 125.6. Matches aSDomain:
                     nrpspksdomains_sapJ_PKS_KS.1"
                     /codon_start=1
                     /gene="sapJ"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     arylpolyene: APE_KS2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 175.6; E-value:
                     2.9e-53)"
                     /gene_kind="biosynthetic"
                     /note="Beta-ketoacyl_synthase"
                     /product="SapJ"
                     /protein_id="QMX85617.1"
                     /sec_met_domain="APE_KS2 (E-value: 4.6e-47, bitscore:
                     150.7, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHVAIIGADLLTCHGGTDSTFAALAKGVDGSQNLRFYDTDRINVT
                     RGYHVEDSTDAESEPPLRAGRWLARCVLGAVRQAGVDPAAERVSVIVGTGLRELRAMER
                     WALDGIPVRRRDLHFTTAVREVLPEVTEVITVSNACSASGHALALAQDLLAAGEADAVV
                     AAGCDAMSESMLTMIGRVADAPTPALRPFQEGRAGVLLGEGAVAVVLRRAADAGPGALA
                     TVLATGLSCDAHHETAPDVDGIVAAMRDAHRRAEVTPHDIGLVLAHGTGTALNDPTESA
                     ALDTVFAGAAPGPLVTGIKGAIGHTSGSAALMSLLVAAWAMRSGSVPATAGLAEPIEAA
                     AGLRLVIGDPAPCDARVAQVNAFGFGGVNAVSVIGVDR"
     aSDomain        7482..8210
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_sapJ_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="7.60E-39"
                     /label="sapJ_PKS_KS.1"
                     /locus_tag="sapJ"
                     /protein_end="374"
                     /protein_start="131"
                     /score="125.6"
                     /tool="antismash"
                     /translation="EVITVSNACSASGHALALAQDLLAAGEADAVVAAGCDAMSESMLT
                     MIGRVADAPTPALRPFQEGRAGVLLGEGAVAVVLRRAADAGPGALATVLATGLSCDAHH
                     ETAPDVDGIVAAMRDAHRRAEVTPHDIGLVLAHGTGTALNDPTESAALDTVFAGAAPGP
                     LVTGIKGAIGHTSGSAALMSLLVAAWAMRSGSVPATAGLAEPIEAAAGLRLVIGDPAPC
                     DARVAQVNAFGFGGVNAVSVI"
     gene            8228..9199
                     /gene="sapK"
     CDS             8228..9199
                     /codon_start=1
                     /gene="sapK"
                     /note="Beta-ketoacyl_synthase"
                     /product="SapK"
                     /protein_id="QMX85618.1"
                     /transl_table=11
                     /translation="MNPDGVTVTAHALHVPDPIGGSVATAAGDLFSSVAPEPACGPDQA
                     HTVLGRKGLLFKEDATRLALCAVHRAFGLPPGKLTEPLPGAEHTAVVVSSNVGNARTVR
                     DIVAGMRAGSAHDVSPLNGPNASSNVIASTLAIRYGFTGPNLMVCSGATSGLDAVRLGA
                     LLLRGERARRVVVVGAEPDDEVARGLAALRPAAPGTTLPVPLRAAAACVVLEHGGAGPR
                     LGNVRRHTRPDDIADDLAPPAALRLTAPGAPGAGGGIDLTAHLGEVYGALGVVQLAVAA
                     AWLGSGQPSGPGGALITCGDGEDGYASVRLHPADVPALAGGG"
     gene            9201..10103
                     /gene="sapL"
     CDS             9201..10103
                     /codon_start=1
                     /gene="sapL"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /note="haloalkane_dehalogenase"
                     /product="SapL"
                     /protein_id="QMX85619.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 8.8e-17, bitscore:
                     52.3, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEPNGPRPGPPPDRWTAVAEARGARERLAVLGTPSPAPHPALPV
                     AALVHGLEGGWAGWQPLAGELAGHCRTYALDLPWRAGNRYRWRHHGTPAHWLREALALV
                     PEPVDILIGHSFGANAVLEHLADRDGTPRPCAAVLLAPFYRPETLRVDWPLYDAALAGF
                     RQIIADGVRVGLGRRAGDLDPEVLAAMADTTVERIGPVGFLSLFLQFTGTTELDLGAVT
                     VPTLVLAGHGDASLSEDRAAALAAAMPAATVRRRPGYTHFCHAEQPGDVGADIARFLDG
                     RTALPATTPLATTLLTTEE"
     gene            10106..11059
                     /gene="sapM"
     CDS             10106..11059
                     /codon_start=1
                     /gene="sapM"
                     /note="thioesterase"
                     /product="SapM"
                     /protein_id="QMX85620.1"
                     /transl_table=11
                     /translation="MTELLDGTLTTPVGRPRYEGANIRTWIGFKHFMYLTEEAVLQYFR
                     ERGVGAETLHHTYGLGLEIVDHSVSLPATLGIDDEVTAVVEPGKPKPGHGAPFKVRLTV
                     ERDGATVTVLTGRVRVALITLKDAPAGIQPVPAHLEPYTVPEVAALADAVDHEPRTVAE
                     SDLPAVLAPADSGAFLWSWRIPYFYCHFSDRLQHSGYVRAMEEVVDRFLADRGISIRTM
                     LVERGWIPVVSRARVHMLADALMEETLHTVFTVREIVKDVMYTATFDTYVHRDGRLVHT
                     ATGTIMHGYAVSRGELAGTLAEFDDITRAALLGETR"
     gene            11056..11781
                     /gene="sapN"
     CDS             11056..11781
                     /codon_start=1
                     /gene="sapN"
                     /note="2-hydroxychromene-2-carboxylate (HCCA) isomerase"
                     /product="SapN"
                     /protein_id="QMX85621.1"
                     /transl_table=11
                     /translation="MKRPPRLYFNLRSPFSWMGLKRLEERFPQAPEVIEYYPYWDPDEK
                     TRAALEERDSGFHYTQMSKAKHLYILQDTKRLSARFGYTMSWPVDIDPWWEVPHLAWLK
                     ARHLGREREFYTAVTAARWERGENICDAAVLHRVAEAAGFDGDAMVAAVHDPALRAEGV
                     DALAAAYDDDVFGIPYFKVGRHRFWGLDRVDGFLDELIPALRKAGVDVADPEFADDEVP
                     AALRERVGAYDSDSAGGCG"
ORIGIN
        1 atgtccgaag cgcgtgagca gcacctggac gagctgcgcg agatcgtcgc cgaggtgctc
       61 gaggtcgaac cggaggagat caccgagacc ggttcgttcg cggaggagca cgaggcggac
      121 tcgctgcggg cgatcgagat cctcgcccgg atcgagaaga agtacaagat cgacatcccg
      181 cagtccgagc tgccccagat ggagaacctc aaggcggtct acgacgtcgt ggcccagcag
      241 gccggctggc aggactgacc ccttcgcaca ccacggcgac gacgacgtca cgagggaaga
      301 aagcggacca ccgacatgaa cgctccaaca gcccgggaca ccgggggacg gcagatggcc
      361 tgggccgggt acacggccgc ggtactggcc gtcgtcttcg gcctgttcag cttctactgg
      421 gcggccgggg gcaccttcgg cctcgacacc ttgggcggcg cgatccagcg gatggccgaa
      481 cgccgcgact ccggcttcat cgcggtcgtc tggatcaccg gcgcgctcaa ggtggcgggc
      541 ggtctgttct cgttgtcgct cgtccggccg tggagccgtg tcgtcccccg cccgctgatg
      601 cttctcgcgg gctggggcgg cgcggcgctg ctgaccgtct acggcgcttt gcaggtcggc
      661 agcctgtcgc tcgtggcgtt cggcgtgctc accccgtccg accccgtcga gtggaagccg
      721 ctgctgtggc gcctgttcgt ctgggacatg tcgttcctgg tgtggggaat cctgctcttc
      781 ctcgccaccc ggacctatgt catgaaccat ccgcggcgcc cggaaaggcg cggaggaaat
      841 tgatgtacga cagcgcgcag gacctggtct tcgaccgtgt cttcctgcga atcgatggac
      901 tcgtccccga gatccggctc ctgctcaaga tcgagggttt caatccggcc ggttcgatca
      961 aactgaagac cgccatcgga ctcgtcgagg acgccgagcg gtggaatctg ctcagacccg
     1021 gcggccacat catcgaatcg tcctcgggca acctgggagt ggcgctcgcc tccgtgtgcg
     1081 ccgccagggg ataccgcttc acctgtgtca ccgacccgaa cacctcgccc cagaacgcgg
     1141 ccctgatccg ggccttcggg gcgacggtgg tggtcgtcga cacgaaggac gccaacggcg
     1201 ggtatctaca gtccaggatc gactacatcc acgaccggct gcgggaggac ccctccctgt
     1261 tctggccgaa ccagtacgcc aatgaggcca acccccgtat ccaccgggac cgcaccgccg
     1321 ccgccatcct cgacgaggtc cccgaggtcg accacctgtt catcggcgcc ggtaccaccg
     1381 gcaccctgat gggctgcgcg gcccacttcc gcgaacgctc cccggacacc cggatcgtcg
     1441 cggtggacac cgccggatcc gtcaccttcg gcttcccgcc cggcaagcgg catctcccgg
     1501 gactcggcac cagccgcaag cccgaactgt gcgaccccag gctcgtcgac gacatcgtca
     1561 tcgtccccga ggccgaggcg gtccgcatgt gccgcaccat cgccagaagc cggggcatcg
     1621 ccaccggcgc ctccaccggc tcggtcctgg cggccgtggt gcgcaggggc ccggagctgc
     1681 cgcccggatc caccgtggtg gccatcgccc ccgacaccgg ggaccgctat ctcaacaccg
     1741 tctacaacga cacctgggtc gaggaacgct tcggacacca ggccctgatc ccggcgggcg
     1801 cggacgacca tccggacgcc gacggctcac tgctcctgaa aggctgacat gttcgacttc
     1861 cacgtcgtca gcggcgaaac cgcccgccgt gtgatcggcc ggtcccggtc gcacatcgtc
     1921 gaccaggtac gggacaccta tctcacccat gacctgggtg actcggtcaa tcccaacagc
     1981 tacttcctgc gcttccccga caagcccgac agccgcatca tcgccctgcc cgcctatctg
     2041 ggcggtgaac actcggtcgc cggcatcaag tggatcgcca gcttccccgc caatatcgag
     2101 cgttcggtgc cgcgcgcctc ggcggtcctg ctgctcaacg accacgagac cggctatccg
     2161 ttcgcctgtc tcgaagccgc ccagatcagc gccgcccgca ccgcggcctc cgccgtgctc
     2221 gccgccgaga cgctgcacgg atcgcggacc gcccggcggc tgctggtgat cggcgccggc
     2281 atcatcgccc gcaacatcct ggagttcttc accgaccgcg actggcagct cgacgaggtg
     2341 ctggtgcacg accgggaccc gcgctacgcc gaatcgctca ccgagcatgt gtcgggcgca
     2401 ctcggacacc gtgcgcgcgt cgcccccgac cgggacaagg cgatcgcgga ggccgatctg
     2461 gtggtgctcg ccaccacggc cgcagagccc cacatcaccc gtccgggcac cttcgccccc
     2521 ggtcagatcg tcctcaacat ctccctgcgg gacatcgacc cggagatcat cgtgggcgcc
     2581 cacaacatcc tggacgacat cgaccactgc ctcaccgcca acacctcgcc gcatctggcc
     2641 gagcagaagt tcggccaccg ggaattcatc aacggcaccc tcgcggatgt gatccgcggc
     2701 cgtcggaagc tggccaccga ccggcccgtc atcttctcgc ccttcggcct cggcgtgctc
     2761 gacctcgccg tcggcgcgtt cgtgcaccgg gccgccctgg aggagaacga ggccaccgcc
     2821 atcgaggggt tcttcggcga gaccacccgc tggtgagcgt cacccgctgg tgagcgcgcc
     2881 cgctcatgac ccgccgtccc gtaccaccca ctgacccgta tccggacccg acccacgagg
     2941 taattcccat gtccacacaa cgccgtgccc ttgtcaccgg gggatcgcgc ggtatcggcg
     3001 cgagcgtcgc cctccgcctc gcccaggacg gaatggacgt cgcgttctgc taccgcagcg
     3061 agaaggagcg cgccgaggag accgccgagc ggatccgcga gctgggcgtg cgctgcttcc
     3121 acgcaccctg tgacgtcgcg gacttcgagg ccgtgcagtc cttcatcaag gaggccgagg
     3181 ccgacctcgg cgccatcgac accctcgtca actcggccgg tatcgtccgc gacaaccctc
     3241 tcgtgctgat gccggtggag gactgggacg ccgtcatcga cgccagcctg accggtgtct
     3301 tccacttctg ccggtcgatg gcgttcggct tcatgaaacg caagcggggc gtcatcgtca
     3361 acgtctcctc ggtggccggc gtctacggca acaaggcgca gacgaactac tccgccgcca
     3421 aggccggtat caacggcatg agcaaggcgc tggccaagga gctcgccccc tacggcctgc
     3481 gggtcaatgt cgtcgcccct ggattcatcg agaccgacat gaccgcgggc ctcaccgaca
     3541 aggtgcgcgc ccaggcgctg gaactggtcc ccctgcgccg cttcggtacc cccgaggacg
     3601 tggccgaact ggtgtcgttc ctggtgtccg accgggcggg ctacatcacc gggcagatcc
     3661 tccaggtcga cgggggcatc gtcctgtgag ccgccggttc gccgccccgc tcaccgccgt
     3721 cgacacggtg gacatcgcgg acacccccgc cggcacacgg gtggaggcgg tcaagcggat
     3781 caccggcgac gaaccgtatc tcgtcggcca ctacccggac ttcaccatct accccggggt
     3841 gttcaccctc gaatccgtcc accaggccat ccgccactgg gccaccgaac ggcacggccc
     3901 cggcgcgctc gccgaacttg ccaccatcac ctcgatcagc ttcgcggccc cgctcctgcc
     3961 cggggacacc ctgcgcatcg gctgcgagct gaccgaacgc gatgacgcac ccgcggcggt
     4021 gcgggccaag gtccgctgcc accgcggcga cggcgccctg tccgcccgga tgaccctcga
     4081 actgcgcatc gaccggaggg acggcgatga ccacgcttga acacgccgac atccggcgca
     4141 tcctgccgca ccgccacccc gttctgctcg tggaccgcgt cctggacctc gaacccgggg
     4201 tgcgcatcgt cgccaccaag gccgtgaccg gcggcgagcc ctgctacgcc ggtctggacg
     4261 aggacctccc cgtctccgcc tacgcctatc cggcctccct gctcgtcgag tcattcggcc
     4321 agaccggcgc cgtgctgtgg ctgtactcgg cagccacctc cggggagcgg cgcgagggca
     4381 ccctcatctt cggctccgcc cgcgaggtga cgatcaccgg cagcgcctac cccggcgatg
     4441 tgctgcgtca cacggtcgag atcgaccagc tcatcggcga caacgcgttc atgcgcggcg
     4501 agacctgggt gggcgaccgc cgtatcgcca ccctcggctc ggtcctcgcc gtcgtacgcg
     4561 aagggctcgc cgccggcacc ggctcatgaa ccctccgcac gctccccagg cccaggtttg
     4621 tgcccgccac ccccgttccc gccagattca ggagaaatcc catgtccgaa gcgcgtcaga
     4681 acggtccacg acgtcgtggc cacccaggtc ggccggcagg accgatcggc atgaaccgac
     4741 gggtcgtcat caccgggctc ggcccggtct ccagcatcgg caccggtgtc acggcgttcg
     4801 gccgggccct gcgcgccggc accagtggca tctccgccat ctccagcttc gactcctccg
     4861 gcttcccgca ctacatggcc ggtgaggtcc ccgacttcga ccccaaggcg ctgctgcgca
     4921 ccctctccac cgccgagtgg ggccgcacca gcctgttcgc cgccgcggcc gcgcggctgg
     4981 ccgtcgcgga cgccggaatc gacgagaagg tgctcgcggg cgccgccgcc ggctccagta
     5041 tcggcaccac cagcggtgag tcccaggtca tcgaggcgct caccgccgaa tgggtgcaca
     5101 ccggactggc cgaggtcacc cccgcgtacg ccgcccaggt cccggcctcc cggctggcca
     5161 ccgccgtcaa ccaggaactc ggcctcaccg gcgaggccgt gaccctgtcc accgcctgct
     5221 cggccagcaa ctacgcactc ggctacgcct acgaccagat cgtcaccggc gacgccgact
     5281 acatgatcgc cggtggcgcg gactcggtgt gccgctgggc gcacgccggt ttctaccggc
     5341 tcggcgcgct caccgagaag gtctgctcac cgttcgacaa ggaccgctcc ggcatcctca
     5401 ccggcgaggg cggggccgcc ctcttcctgg agaccctgga gtccgccacc ggacgcggcg
     5461 cccacatcta cgccgaggtg ctcggctacg gcctcaactg cgacgccaac cacatggtcg
     5521 cccccgaccc ggtgagcatc gccgagtgca tgcgccgggc ccaccacaac gccggtatcg
     5581 cgcccggcga cgtcgactac atctgcgccc acggcaccgg caccccggcc aacgacgcca
     5641 tggaggcccg ggccgtggtc gaggtcttcg gcacccggcc cccgccgatc agctccatca
     5701 aatccatgct cggccacacc atgggcgcgg ccagcggatt cggcgccatc gccgccgcac
     5761 tcgccatcga caccggcttc ctgcccccga ccaccaacta ccgcaccccc gaccccgcac
     5821 tgcccgccat cgaccccgtg ccgaacgaga gccgcccggc acggcccgag gtcgtgcaga
     5881 acaacggctt cgccttcggc ggcaacaacg ccatcaccat cctcggacgg gtcgcatgaa
     5941 cgccaccgcc accagcccgg acctcaccgg ccacggggac gtccgcggaa acaccctggc
     6001 catcaccggc tggggcgtcg tcacctccct ggccatcggc gccgaggagt tcaccaccgc
     6061 catcggcgag ggccgcagcg ggctgcgcga cgtcaccggc atgttcgacg aagagctgcc
     6121 cgagaaccag gcgttcgcca tgcccgactt cacggtgcgc gaccacctcg gccgcaaggg
     6181 caccagcttc ttcgaccgca gcacctccct cggactgctc ggctgcaccc tcgccctgga
     6241 cgacaccgac ctgaccgtca ccgatgacaa ccgcgaccgg ttcggcatcg tcctgggcac
     6301 caccgccggc agcatcaagg ccaccagcga ctacagccgc gaaacactgg agggcgaccg
     6361 gccgttcctg gtcaacccgg tgctgttccc caatacggtg atgaactgcg ccgccggcca
     6421 gtccgcgatc tggctggggc tcaagggcgt caacgccacc gtggcgggcg gtcggctcgc
     6481 cgggctgagc gccctgcgct acgcccgcaa ccagatccgc cgcggctacg cggatgccct
     6541 gctcgtcggc tccgtcgagg agttcagccc gcacaccgcc tggttcagcc accgccagca
     6601 ctccgccgag gaagaccggg tcacggtcgg cgagggctcc gccgtcttcg tcgtggagaa
     6661 cgcggaggcg gtgcgcgccc agggacgcac ccccgacgcc gaggtgctgg ccgtcgaggt
     6721 cggcacctac ggcgcccccg gccgggagcc cgacgccgga gccggactcg ccgaccgcat
     6781 cggccgcgcc ctgcgccgcg ccggagtgga accgtcccag gtacgggcgg tcgccaccgg
     6841 cgagagcgga atcgcccgcc tggacgacat cgagcgccgt ggcatcgaga gcgcgctcgg
     6901 cgccgaggtg cggcgggtgc gggccaagga gtgggtcggc gagtgccaga gcgcctccgg
     6961 ttcgcttcag atcgccgccc tgctggccgg tcaccgcggt cacccggagc tggacggcgc
     7021 ggtgtccgtg gtcacctcgg tctctcccga cggcggtgtc ggcgcggccg tactgagggg
     7081 gtggcaccgt gcacgtggcg atcatcggag ctgatctgct cacctgccac ggcggcaccg
     7141 actccacctt cgccgccctg gcgaagggcg tcgacggcag ccagaacctg aggttctacg
     7201 acaccgaccg catcaacgtc acccgcggct atcacgtcga ggactccacc gacgccgagt
     7261 ccgaaccgcc gctgcgggcc gggcgctggc tcgcccgctg cgtcctcggc gccgtccgcc
     7321 aggccggggt ggacccggcc gccgagcggg tgtccgtcat cgtcggcacc ggactgcgcg
     7381 aactgcgggc catggagcgc tgggccctgg acgggatccc ggtgcgccgc cgggatctgc
     7441 acttcaccac cgcggtgcgc gaggtgctgc ccgaggtcac cgaggtgatc acggtgtcca
     7501 acgcctgctc cgcgtccggc catgccctcg cgctcgccca ggacctgctc gcggcgggcg
     7561 aggccgacgc ggtcgtggcg gcgggctgcg acgcgatgag cgaatccatg ctcaccatga
     7621 tcggccgggt ggccgacgcg cccacgcccg cgctccggcc cttccaggag ggccgcgccg
     7681 gtgtgctgct gggcgagggc gcggtggcgg tggtcctgcg gcgggccgcc gacgccgggc
     7741 ccggcgcgct cgccaccgtc ctcgccaccg ggctctcctg cgacgcccac cacgagaccg
     7801 ccccggacgt ggacgggatc gtcgcggcca tgcgggacgc ccaccgccgc gccgaggtca
     7861 ccccgcacga catcggcctg gtcctcgccc atggcaccgg caccgcgctc aacgacccga
     7921 ccgaatccgc cgccttggac acggtcttcg ccggtgccgc cccgggcccg ctggtgaccg
     7981 gcatcaaggg agccatcggg cacacctccg gcagcgcggc gctgatgagc ctgctcgtcg
     8041 ccgcctgggc gatgcgttcg ggttcggtgc ccgccacggc ggggctcgcc gagccgatcg
     8101 aggcggccgc cggactgcgg ctggtcatcg gcgaccccgc gccctgcgac gcccgggtgg
     8161 cccaggtcaa tgccttcgga ttcggcggag tcaacgccgt cagcgtgatc ggggtggacc
     8221 gatgaccatg aaccccgacg gggtgaccgt caccgcacac gccctccatg tgcccgaccc
     8281 gatcggcggc tccgtggcga ccgcggcggg ggacctcttc tcctccgtgg ccccggaacc
     8341 ggcctgcggc ccggaccagg cgcacaccgt actgggccgc aaggggctgc tgttcaagga
     8401 ggacgcgacc cggctggcgc tgtgcgcggt ccaccgtgcc ttcggactgc ccccgggcaa
     8461 gctcaccgaa ccgctccccg gagcggaaca caccgccgtc gtcgtcagct ccaacgtcgg
     8521 caacgcccgg acagtccgcg acatcgtggc cgggatgcgg gccggctccg cccacgacgt
     8581 ctccccgctg aacggcccca acgcctccag caatgtgatc gccagtaccc tcgccatccg
     8641 ctacggcttc accgggccca atctgatggt gtgctccggc gccacctccg gcctggacgc
     8701 cgtccggctg ggcgccctgc tgctgcgcgg cgagcgcgcc cgccgggtcg tggtggtcgg
     8761 ggccgaaccc gatgacgagg tggcccgcgg gctggccgcc ctccgcccgg ccgcgcccgg
     8821 caccacgctg ccggtcccgc tccgggcggc cgccgcctgc gtcgtactgg agcacggcgg
     8881 ggccgggccg cggctgggga acgtacgccg ccacacccgg ccggacgaca tcgccgacga
     8941 cctcgcgccg cccgccgcgc tccggctcac cgcccccggg gccccgggag ccggcggcgg
     9001 catcgacctc accgcgcatc tgggggaggt ctacggagcg ctcggcgtgg tccaactggc
     9061 cgtggccgcc gcctggttgg gctccgggca gccgtccgga ccgggcgggg cgctgatcac
     9121 ctgcggcgac ggcgaggacg gctacgcctc ggtgcggctc cacccggcgg acgtccccgc
     9181 cctcgcgggg ggcggctgac atggcggagc cgaacgggcc gcgcccggga ccgccgcccg
     9241 accggtggac agcggtggcc gaggcccgcg gggcgcggga gcggctggcc gtgctgggga
     9301 cgccgtcccc ggcaccgcac cccgccctcc ccgtggcggc cctggtgcac ggtctcgaag
     9361 gcgggtgggc cggatggcag ccgctggccg gagagctggc gggccactgc cggacctacg
     9421 cgctcgacct gccgtggcgc gcgggcaacc gctaccgctg gcggcaccac ggcacccccg
     9481 cgcactggct gcgcgaggcg ctcgccctgg tgcccgagcc cgtcgacatc ctgatcggcc
     9541 actccttcgg cgccaacgcg gtcctggagc acctggccga ccgggacggc acgccccggc
     9601 cgtgcgccgc ggtgctgctc gccccgttct accgccccga aaccctccgg gtggactggc
     9661 ccctgtacga cgcggcgctg gcgggcttcc ggcagatcat cgccgacggc gtacgcgtcg
     9721 gactggggag gcgggccggg gacctggacc ccgaggtgct cgccgccatg gccgacacca
     9781 cggtcgagcg gatcggcccg gtcggattcc tcagcctgtt cctccagttc accggcacca
     9841 ccgaactcga cctgggcgcc gtcaccgtgc ccaccctcgt cctggccgga cacggcgacg
     9901 cctcgctgtc cgaggaccgc gcggcggccc tggccgccgc catgcccgcg gcgaccgtcc
     9961 gcagacgccc cggctacacc cacttctgcc atgcggagca gcccggggac gtcggcgcgg
    10021 acatcgcccg cttcctggac ggccgcaccg ccctcccggc gaccaccccc ctcgccacga
    10081 cccttctcac cacagaggaa tgaccatgac ggagctgctg gacggcaccc tcaccacccc
    10141 cgtgggcagg ccccgctacg agggcgccaa catccgcacc tggatcggct tcaagcactt
    10201 catgtatctg acggaggagg cggtcctcca gtacttccgc gagcgcgggg tgggcgcgga
    10261 gacgctccac cacacctacg gcctcggcct ggagatcgtc gaccactcgg tcagcctgcc
    10321 cgccacgctc ggcatcgacg acgaggtgac cgccgtcgtg gagccgggca agcccaagcc
    10381 cggccacggc gccccgttca aggtccggct gaccgtcgag cgggacggcg cgaccgtcac
    10441 cgttctcacc ggcagggtcc gggtcgccct gatcaccctg aaggacgccc ccgccggcat
    10501 ccagcccgtc ccggcccatc tggagcccta caccgtcccc gaggtggccg ccctcgccga
    10561 cgcggtggac cacgagccca ggaccgtcgc cgagagcgac ctgcccgccg tcctcgcccc
    10621 ggccgactca ggcgccttcc tgtggtcctg gcgaatcccg tacttctact gccacttctc
    10681 cgaccggctc cagcactccg gctacgtccg ggccatggag gaggtcgtgg accgcttcct
    10741 ggcggaccgc ggcatctcca tccgcaccat gctcgtcgag cgcggctgga tccccgtggt
    10801 gtcccgggcc cgggtgcaca tgctcgccga cgccctcatg gaagagaccc tgcacaccgt
    10861 gttcaccgtc cgggagatcg tcaaggacgt gatgtacacc gccaccttcg acacctatgt
    10921 ccaccgcgac ggacggctgg tgcacaccgc caccgggacc atcatgcacg gctacgccgt
    10981 cagccgcggt gaactggccg gcaccctcgc cgagttcgac gacatcaccc gcgccgcgct
    11041 gctgggggag acccgatgaa gcgaccgccc cgcctgtact tcaacctgcg ctccccgttc
    11101 agctggatgg ggctcaagcg gctcgaggag cgtttcccgc aggccccgga ggtcatcgag
    11161 tactacccgt actgggatcc ggacgagaag acccgggccg cgctggagga gcgcgactcc
    11221 ggcttccact acacccagat gagcaaggcc aagcacctct acatcctcca ggacaccaag
    11281 cggctctccg cccgcttcgg atacaccatg tcctggccgg tggacatcga cccgtggtgg
    11341 gaggtgccgc atctggcctg gctgaaggcg cgccacctcg gccgtgagcg cgagttctac
    11401 acggcggtga cggcggcccg ctgggaacgt ggcgagaaca tctgcgacgc cgcggtgctg
    11461 caccgggtgg ccgaggcggc cgggttcgac ggcgatgcga tggtggccgc cgtccacgac
    11521 cccgcactgc gcgccgaagg cgtggacgcc ctcgccgccg cctacgacga cgatgtgttc
    11581 ggcatcccgt acttcaaggt cggacgccac cgcttctggg gcctggaccg ggtcgacggc
    11641 ttcctcgacg agctgatccc ggccctgcgg aaggccgggg tggacgtggc cgaccccgag
    11701 ttcgcggacg acgaggtgcc ggccgcgctg cgggagcggg tgggcgccta cgactccgac
    11761 tccgccggcg gctgcggctg a
//
