LOCUS       BGC0002514             16028 bp    DNA     linear   BCT 05-SEP-2020
DEFINITION  Streptomyces sampsonii strain SCSIO 054 julichrome biosynthetic gene
            cluster, complete sequence.
ACCESSION   BGC0002514
VERSION     BGC0002514
KEYWORDS    .
SOURCE      Streptomyces sampsonii
  ORGANISM  Streptomyces sampsonii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces albidoflavus group.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:53:04
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..846)
                     /gene="juiO"
     CDS             complement(1..846)
                     /codon_start=1
                     /gene="juiO"
                     /gene_functions="other (smcogs) SMCOG1199:NmrA family
                     protein (Score: 274.9; E-value: 1.4e-83)"
                     /gene_kind="other"
                     /product="NmrA family protein"
                     /protein_id="QNL10604.1"
                     /transl_table=11
                     /translation="MTFLVTGATGNVGRNVVELLLAAGVPVRATSRSPETAGLPAGVDV
                     RAGDLDDPGSFGDALSGVERLFLFPHPAGVAGFLDLAKASGVRQVVLLSSAAVRESANP
                     IGLMHLSVEEAVRGSGLGWTMLRPGAFASNTLQWAPAIRAGDEVAIPYAESTVSPVHER
                     EIAAVAVQALLSGGHDGATYDVTGPESMTQRRQAELIAAATGRPVTVTDLVGEAARAQL
                     ERQYGAFGVPGLVDSVLATYTASVGTDAPLTDTVTRVTGRPPTPFAAWAAEHRTAFGAA
                     "
     source          1..16028
                     /db_xref="taxon:42239"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sampsonii"
                     /strain="SCSIO 054"
     misc_feature    1..16028
                     /note="julichrome biosynthetic gene cluster"
     protocluster    1..16028
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[10476:12935]"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_malonyl_elongations="8|9 (Score: 667.0; E-value:
                     1.9e-203)"
                     /t2pks_molecular_weights="acetyl-CoA_8 (Da): 349.356"
                     /t2pks_molecular_weights="acetyl-CoA_9 (Da): 391.392"
                     /t2pks_product_classes="angucycline"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
                     /tool="antismash"
     proto_core      10477..12935
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_malonyl_elongations="8|9 (Score: 667.0; E-value:
                     1.9e-203)"
                     /t2pks_molecular_weights="acetyl-CoA_8 (Da): 349.356"
                     /t2pks_molecular_weights="acetyl-CoA_9 (Da): 391.392"
                     /t2pks_product_classes="angucycline"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
     cand_cluster    1..16028
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2ks and t2clf)"
                     /kind="single"
                     /product="T2PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..16028
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002514"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..16028
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T2PKS"
                     /region_number="1"
                     /rules="(t2ks and t2clf)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(887..2347)
                     /gene="juiN"
     CDS             complement(887..2347)
                     /codon_start=1
                     /gene="juiN"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050:monooxygenase FAD-binding (Score: 433.5; E-value:
                     2e-131)"
                     /gene_functions="biosynthetic-additional (t2pks) OXY
                     (Score: 407.1; E-value: 2.6e-124)"
                     /gene_kind="biosynthetic-additional"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="QNL10605.1"
                     /sec_met_domain="DAO (E-value: 8.4e-10, bitscore: 28.6,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDADVIVVGAGPTGLTLAHELALAGVDVALLDKGPGRTGESRALN
                     LHPRTAEILAMRGMLDGAEEQARARLETTHFAHLPVPLALDGWNSRYPYQVAIPQAEVE
                     QVLEEHLAGLGVRVRWNVEVTDIEQDAERIRVQAGGTWVEARYLAGCDGGRSFVRKQLG
                     IAFPGLEARWFGTVSDVVLGVAGEGIATTWDTKGSPRMRPDGSFANIFPIGNESDRVHR
                     IFYSVADQVIDDQRAKVPDTEVVAAIEEFYGSETEVKEVRWASRFSDASRLAEHYRAGR
                     AFLAGDAAHIHLPLGGQGLNTGIQDAMNLGWKLAADVAGWAPEGLLDTYHGERHPVGAA
                     VLTNTRAQVALGQKGVEHDALREIFTQLLALPEANRLLGGAISGLSIRYDLGGDEPVGS
                     RLADMVLPDGRWAGDLFHQGHGVLLTALPDLVAEAAPWTGRVDAVAVRELPGLSAEAVL
                     VRPDGHICWVPGTPLADALHRWFGAPRP"
     misc_feature    complement(2285..2287)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(2456..3619)
                     /gene="juiM"
     CDS             complement(2456..3619)
                     /codon_start=1
                     /gene="juiM"
                     /product="hypothetical protein"
                     /protein_id="QNL10606.1"
                     /transl_table=11
                     /translation="MRQLRVDPPVQLLWRVPGRPDHEALRTLHTSLAEGPLNRRMRTAR
                     LPLARPVWEGHTGCAPLRVSSRAVAPETVLDWADAACRAELDAHAGRGWELSAAPVSDG
                     TFVVSLVGSHALTDGQGLIGAATLAARRTARQWHSGQPSLVDDLTDVVTDGLWTYSRLA
                     LSRLRVKAASVPATAARVLLGRTEQGGGAAPVRSRLAFTVPAAEADRVARNGGGSVTGL
                     MLAMTANILRAARGTTDAGRPFTVGLPVSFRRPDDLESSNMVGIATLRLDGLRGRYTDL
                     VEIRRLSKAVYAEAAGRHLTGYPQTDAALSNAGEMLPEARDVFGPALGVLFRTVSGAGA
                     PPGRMNCFVLRAGATVSVGFQSAGVAVGPGLVEAELDAWGITPAHAW"
     gene            complement(3661..>4662)
                     /gene="juiL"
     CDS             complement(3661..4662)
                     /codon_start=1
                     /gene="juiL"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083:oxidoreductase (Score: 244.2; E-value: 3.3e-74)"
                     /gene_kind="biosynthetic-additional"
                     /product="F420-dependent oxidoreductase"
                     /protein_id="QNL10607.1"
                     /transl_table=11
                     /translation="MSIRLGYHIPYFHHTRTAELFPTLVAQVRTAEASGFDLVTTMDHF
                     YQPPLIGPPEGPMLEAYTLLGALAAVTDRVRLSTMVTGIGYRNPALLAKMVTTLDVISR
                     GRAVLGVGAGWHEREHHDYGYVFGTVAERFSRFEEALGIAVPMLRGSRPQVEGRWFRAE
                     DAVNEPRLRDDLPVLIGGGGEKKTFAFAAEHADHLNIICTTSELPRKMAAVRTRCAEAG
                     RDAAMLETSFATPLIIHEDGDQARKLLEEVLARSGVDVTGLTNSERAAATDRFFVGTPA
                     EVTGQLQERVLCHGVDGLMANMLANAHDPDAIALAGRTLAPLVGLTGTGAAK"
     gene            complement(4675..5901)
                     /gene="juiI"
     CDS             complement(4675..5901)
                     /codon_start=1
                     /gene="juiI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 402.2; E-value:
                     3.8e-122)"
                     /gene_kind="biosynthetic-additional"
                     /product="Cytochrome P450"
                     /protein_id="QNL10608.1"
                     /sec_met_domain="p450 (E-value: 7.9e-20, bitscore: 60.9,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEITAESVPEFPILRTCPFSVPETYRELEGTGGRIHRVRMSDGR
                     PAWLVTKHDDARAVLSDARFSSEKLRPGFPELSPGGLKALTYFSPFLVNMDGPEHAQAR
                     RAVLGEFSVRRINAMRPRIRRIVDAAIDRMLEQPGRPVDLAAHLSQPVPTLVLSAFLGV
                     PAEDLDAIERNTGKMLHEARTEDEQRAAAEALHAHLDLVIAAKEENPGDDFLSRQIDRS
                     RREHGDAADRFELASLVQLLQIAGHASSAAMISLSVLTLLSEPEQLKQLTADPSRTPAA
                     AEELLRFLSITDTGPLRLALEDVEIGGVRIRAGDGVLIPTLPANRDAGAFPDPDRFDIG
                     RLPGTRHVAFGYGAHQCLGQNLVRAELQVVLDRLFHRIPDLRLATAPEALPYKYFGQFF
                     GPVELPVTW"
     gene            complement(6055..6759)
                     /gene="juiK"
     CDS             complement(6055..6759)
                     /codon_start=1
                     /gene="juiK"
                     /gene_functions="regulatory (smcogs) SMCOG1239:TetR family
                     transcriptional regulator (Score: 160.5; E-value: 9.2e-49)"
                     /gene_kind="regulatory"
                     /product="TetR family transcriptional Regulator"
                     /protein_id="QNL10609.1"
                     /transl_table=11
                     /translation="MRQKSNSADVPVPPWEVRRKSGTRVRAPLTAERIVDAALAVVDTE
                     GTSAITMRRIAGDLGVVASSLYAHVRNREELLLLVLDGVMREVGVPELTGQWDADLKAH
                     YTKMQRVLSAHGDIALYNFAAFPPTAIGVAITERLLSVLLDVGVPPRVAAWALHRLTIY
                     TTADVYEGWHLAHKGLDNWIEPVHEYFASLPADRFPAIAGNVDVMLDSDSDGRFELGLD
                     MLISGLATFVEE"
     gene            6971..8434
                     /gene="juiJ"
     CDS             6971..8434
                     /codon_start=1
                     /gene="juiJ"
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 315.2; E-value:
                     9.3e-96)"
                     /gene_kind="transport"
                     /product="MFS transporter"
                     /protein_id="QNL10610.1"
                     /transl_table=11
                     /translation="MSTTETPRAAALPEGPPVSARRAWAVVGIILVGEIMDLLDALVTT
                     VAGPTIMRDMGGSQEFIQWLAAGYTIAMAVGLLIGGRLGDKYGRKNLFLIGLTGFTLTS
                     LVSALATSPETLITARVLQGLLGALMVPQALGIIRSVFPPDKVGLAFGVTGPVMALSGV
                     AGPILAGWLVDADYFGWGWRMIFAVNIPVGVSALVAGLVLLPRSQRQRDLKIDLVGAVM
                     AAAGMGAVIFSLVRGRELGWPWWVGALLLAGVVALAGFVRYQFHRAGRGLPALVVPTLF
                     RKKAFVSGLFVGMLFFSVMMGGALLFSLFFQLGLGMSPLMAGLATIAQAVGMLFGFAFS
                     QALGMARRTMYIGFAVSAAGLLGALGTIAVQSDPVNAWWLSPALAVIGIGSGLAIAPFF
                     DITLSGVDEAETGSASGTLTSAQQVGNALGASVLGTVFFSVLNAHREDGAPAFTTATST
                     ALLVALALVAGAALCTRGLPERAVRHTEV"
     misc_feature    7004..7006
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            <8910..9563
                     /gene="juiG"
     CDS             8910..9563
                     /codon_start=1
                     /gene="juiG"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 156.8; E-value: 1e-47)"
                     /gene_kind="regulatory"
                     /product="Response regulator"
                     /protein_id="QNL10611.1"
                     /transl_table=11
                     /translation="MESEQHQRGVLSQALNRHVHQVTCVDTGAEALDVYDQADVVLLNP
                     NLPDIDGLEVCRAIQATCGIPIIVITDRSSELDCVLALQAGADDYVVRPYGLRELMARM
                     DAVMRRASPRRQSEHVIEHGPLRIDTHTREVRLCDRTIPMSRKEFDLLHLLAVNRHSVV
                     PRERIMRQVWENSWSRRTVDTHVSSIRHKLGGNSWILTVRGVGFRIGQGAPAVP"
     gene            9809..10480
                     /gene="juiH"
     CDS             9809..10480
                     /codon_start=1
                     /gene="juiH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1225:Polyketide synthesis cyclase (Score: 112.3;
                     E-value: 1.8e-34)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC
                     C4-C17/C2-C19 (Score: 155.6; E-value: 3e-48)"
                     /gene_kind="biosynthetic-additional"
                     /product="Polyketide synthesis cyclase"
                     /protein_id="QNL10612.1"
                     /transl_table=11
                     /translation="MFTAVSVGRLERDVRDEISEIFADFDTATEHGASGLRRRQLYAYY
                     DIFIHLQDFTGENAAAAARDAVEGPGTARVRERLARLVPAYGPAGDEDRPTAFYHWEGE
                     PPVDKETGLHTTVIVNRVADEVIPEVARLFGGLDATGFPHRMGTRRRQLFAYQGVYFHL
                     QDFPGEDGTAVIGQAWREADPRFVQICADLDPLVKKYAPERWRSTADQIATRFYHWEAA
                     E"
     gene            <10477..11748
                     /gene="juiA"
     CDS             10477..11748
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (6-397). E-value:
                     9.6e-51. Score: 164.8. Matches aSDomain:
                     nrpspksdomains_juiA_PKS_KS.1"
                     /codon_start=1
                     /gene="juiA"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2ks"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 488.9; E-value:
                     2.2e-148)"
                     /gene_functions="biosynthetic-additional (t2pks) KS (Score:
                     778.9; E-value: 2.5e-237)"
                     /gene_kind="biosynthetic"
                     /product="Beta-ketoacyl synthase"
                     /protein_id="QNL10613.1"
                     /sec_met_domain="t2ks (E-value: 6.5e-247, bitscore: 809.2,
                     seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTARRVVITGVEVIAPGGVGSGKFWELLTDGRTATRDITFFDPSP
                     FRSRVAAEVDLDPYAHGLTPQEVRRMDRAAQFAVVAARGAVADSGLALDALDPYRTGVT
                     VGTAVGSTMSLDQDYRIVSNDGRLDLVDHTYADPHFYQYFVPSSFAAEVARDVGAQGPS
                     GVVSAGCTSGLDSVGYAAELIREGTVDVVVAGATDAPISPITLACFDAIKATTPRNDDP
                     EHASRPFDGTRNGFVLGEGAAMFVLEERESARRRGARIYAEIAGYATRSNAYHMTGLRA
                     DGAEMAEAITVALDEARMNPTEIDYINAHGSGTRQNDRHETAAFKRSLGDHAYRTPVSS
                     IKSMVGHSLGAIGSIEIAASVLAIRNNVVPPTANLHTPDPECDLDYVPLTARDQLTDAV
                     LTVGSGFGGFQSAMVLAQPERSMA"
     aSDomain        10495..11667
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_juiA_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="9.60E-51"
                     /label="juiA_PKS_KS.1"
                     /locus_tag="juiA"
                     /protein_end="397"
                     /protein_start="6"
                     /score="164.8"
                     /tool="antismash"
                     /translation="VITGVEVIAPGGVGSGKFWELLTDGRTATRDITFFDPSPFRSRVA
                     AEVDLDPYAHGLTPQEVRRMDRAAQFAVVAARGAVADSGLALDALDPYRTGVTVGTAVG
                     STMSLDQDYRIVSNDGRLDLVDHTYADPHFYQYFVPSSFAAEVARDVGAQGPSGVVSAG
                     CTSGLDSVGYAAELIREGTVDVVVAGATDAPISPITLACFDAIKATTPRNDDPEHASRP
                     FDGTRNGFVLGEGAAMFVLEERESARRRGARIYAEIAGYATRSNAYHMTGLRADGAEMA
                     EAITVALDEARMNPTEIDYINAHGSGTRQNDRHETAAFKRSLGDHAYRTPVSSIKSMVG
                     HSLGAIGSIEIAASVLAIRNNVVPPTANLHTPDPECDLDYVPLTARDQLTD"
     gene            <11745..12935
                     /gene="juiB"
     CDS             11745..12935
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (67-393). E-value:
                     4.5e-30. Score: 96.7. Matches aSDomain:
                     nrpspksdomains_juiB_PKS_KS.1"
                     /codon_start=1
                     /gene="juiB"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 635.7; E-value:
                     4.3e-193)"
                     /gene_functions="biosynthetic-additional (t2pks) CLF 8|9
                     (Score: 667.0; E-value: 1.9e-203)"
                     /gene_kind="biosynthetic"
                     /product="Beta-ketoacyl synthase"
                     /protein_id="QNL10614.1"
                     /sec_met_domain="t2clf (E-value: 7.6e-200, bitscore: 653.6,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSASVVVTGLGVAAPNGIGEDFWTATLAGKSGIGRLTRFDASRYP
                     ARLAGEIDDLTAEEHLPSRLIPQTDRVTRLSLVAADRALADARVRPAELPEFSMGVSTA
                     SSAGGFAFSQNELRNLWSKGSQYVSAYQSFAWFYAVNTGQISIRHGMKGPSGVVVTDQA
                     GGLDAVAQARRQIRKGTSLVVAGAVDALLCPWGWVGLHTSGRLSTAEEPARAYLPFDDR
                     AAGHVAGEGGALLIVESGASARARGAEVYGEIAGYAATFDGREPGLRKAVELALADAGA
                     SPSDVDVVFADAAAEPELDRVEAEAITKVFGAYGVPVTAPKTMTGRLYAGGAALDLAAA
                     FLALRESTIPPTLHTELAERYGLDLVVSQPRPARLRTALVLARGHGGFNSAMLVRS"
     aSDomain        11946..12923
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_juiB_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="4.50E-30"
                     /label="juiB_PKS_KS.1"
                     /locus_tag="juiB"
                     /protein_end="393"
                     /protein_start="67"
                     /score="96.7"
                     /tool="antismash"
                     /translation="QTDRVTRLSLVAADRALADARVRPAELPEFSMGVSTASSAGGFAF
                     SQNELRNLWSKGSQYVSAYQSFAWFYAVNTGQISIRHGMKGPSGVVVTDQAGGLDAVAQ
                     ARRQIRKGTSLVVAGAVDALLCPWGWVGLHTSGRLSTAEEPARAYLPFDDRAAGHVAGE
                     GGALLIVESGASARARGAEVYGEIAGYAATFDGREPGLRKAVELALADAGASPSDVDVV
                     FADAAAEPELDRVEAEAITKVFGAYGVPVTAPKTMTGRLYAGGAALDLAAAFLALREST
                     IPPTLHTELAERYGLDLVVSQPRPARLRTALVLARGHGGFNSAML"
     gene            13028..13291
                     /gene="juiC"
     CDS             13028..13291
                     /NRPS_PKS="Domain: ACP (22-81). E-value: 3.6e-16. Score:
                     51.2. Matches aSDomain: nrpspksdomains_juiC_ACP.1"
                     /codon_start=1
                     /gene="juiC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1129:acyl carrier protein (Score: 112.2; E-value:
                     1.6e-34)"
                     /gene_functions="biosynthetic-additional (t2pks) ACP
                     (Score: 123.7; E-value: 5.5e-39)"
                     /gene_kind="biosynthetic-additional"
                     /product="Acyl carrier protein"
                     /protein_id="QNL10615.1"
                     /sec_met_domain="PP-binding (E-value: 1.3e-11, bitscore:
                     35.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRTFTLDDLKRILLEGSGAPDGADLDGDILDTEFTDIGYDSLAL
                     LETTSRVEREYGIELDESLVGDASTPRALLDAVSAHLQAQPA"
     aSDomain        13094..13270
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_juiC_ACP.1"
                     /evalue="3.60E-16"
                     /label="juiC_ACP.1"
                     /locus_tag="juiC"
                     /protein_end="81"
                     /protein_start="22"
                     /score="51.2"
                     /tool="antismash"
                     /translation="GADLDGDILDTEFTDIGYDSLALLETTSRVEREYGIELDESLVGD
                     ASTPRALLDAVSAH"
     gene            13358..14143
                     /gene="juiD"
     CDS             13358..14143
                     /NRPS_PKS="Domain: PKS_KR (7-162). E-value: 2.7e-09. Score:
                     29.0. Matches aSDomain: nrpspksdomains_juiD_PKS_KR.1"
                     /codon_start=1
                     /gene="juiD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     253; E-value: 4.8e-77)"
                     /gene_functions="biosynthetic-additional (t2pks) KR C9
                     (Score: 455.4; E-value: 1.3e-139)"
                     /gene_kind="biosynthetic-additional"
                     /product="Ketoacyl reductase"
                     /protein_id="QNL10616.1"
                     /sec_met_domain="adh_short (E-value: 1.4e-36, bitscore:
                     116.1, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAQQSRVVLVTGATSGIGLSVTRLLAGQGHRVYIGSRAEDNVVR
                     TVKQLQEEGLDVDGTVLDVRSGDSVRGFVQAAVERYGTVDVLVNNAGIPGGGVTADVSD
                     ALWDDIIDTNLNSVFRMTREVLTTGGLRHKEWGRIINIASTAGKQGVVLGAPYSASKHG
                     VVGFTKALGNELAPTGITVNAVCPGYVETPMAEQVRRNYSRISGAPESAILEKFQAKIP
                     LGRYSTPEEVAGLAGYLVTDAAASITAQALNVCGGLGNF"
     CDS_motif       13379..13453
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_juiD_0001"
                     /evalue="3.30E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="juiD"
                     /protein_end="32"
                     /protein_start="7"
                     /score="19.8"
                     /tool="antismash"
                     /translation="VVLVTGATSGIGLSVTRLLAGQGHR"
     aSDomain        13379..13843
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_juiD_PKS_KR.1"
                     /evalue="2.70E-09"
                     /label="juiD_PKS_KR.1"
                     /locus_tag="juiD"
                     /protein_end="162"
                     /protein_start="7"
                     /score="29.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VVLVTGATSGIGLSVTRLLAGQGHRVYIGSRAEDNVVRTVKQLQE
                     EGLDVDGTVLDVRSGDSVRGFVQAAVERYGTVDVLVNNAGIPGGGVTADVSDALWDDII
                     DTNLNSVFRMTREVLTTGGLRHKEWGRIINIASTAGKQGVVLGAPYSASKH"
     gene            14173..15123
                     /gene="juiE"
     CDS             14173..15123
                     /NRPS_PKS="Domain: Polyketide_cyc2 (5-146). E-value:
                     2.5e-14. Score: 45.7. Matches aSDomain:
                     nrpspksdomains_juiE_Polyketide_cyc2.1"
                     /NRPS_PKS="Domain: Polyketide_cyc2 (155-283). E-value:
                     3.5e-10. Score: 32.3. Matches aSDomain:
                     nrpspksdomains_juiE_Polyketide_cyc2.2"
                     /codon_start=1
                     /gene="juiE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1128:cyclase/dehydrase (Score: 414.6; E-value:
                     4.3e-126)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C7-C12
                     (Score: 448.2; E-value: 2.8e-137)"
                     /gene_kind="biosynthetic-additional"
                     /product="Cyclase/dehydrase"
                     /protein_id="QNL10617.1"
                     /transl_table=11
                     /translation="MASKPHTARHEIKISAPAETVYRMLAQAEEWPRLFPPNIYVAHVE
                     RGQSEERIRIWATAGDSVKNWTSHRVLDAENLSIRFRQEVSTPPVAAMGGTWHVEALAA
                     GDTLVRLDHDYQAVDEEGLGWIDEVVDRNSRAELESLKTIAESDGAVAGLSCSFEDTVA
                     IGGSAQDVYDFIDRADLWTDRLPHVRAVRLEEPEPGLQTLEMDTLSKDGTSHTTKSYRV
                     CLPHHRIAYKQVTLPALITLHTGRWTVTPGANGVTASSQHTFTVDPGKVTKILGADATV
                     QDAIDYVRTALSTNSMATLGLAKAFAEGRAGGDRR"
     aSDomain        14188..14610
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_juiE_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="2.50E-14"
                     /label="juiE_Polyketide_cyc2.1"
                     /locus_tag="juiE"
                     /protein_end="146"
                     /protein_start="5"
                     /score="45.7"
                     /tool="antismash"
                     /translation="HTARHEIKISAPAETVYRMLAQAEEWPRLFPPNIYVAHVERGQSE
                     ERIRIWATAGDSVKNWTSHRVLDAENLSIRFRQEVSTPPVAAMGGTWHVEALAAGDTLV
                     RLDHDYQAVDEEGLGWIDEVVDRNSRAELESLKTIAE"
     aSModule        14188..15021
                     /domains="nrpspksdomains_juiE_Polyketide_cyc2.1"
                     /domains="nrpspksdomains_juiE_Polyketide_cyc2.2"
                     /incomplete
                     /locus_tags="juiE"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        14638..15021
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_juiE_Polyketide_cyc2.2"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="3.50E-10"
                     /label="juiE_Polyketide_cyc2.2"
                     /locus_tag="juiE"
                     /protein_end="283"
                     /protein_start="155"
                     /score="32.3"
                     /tool="antismash"
                     /translation="CSFEDTVAIGGSAQDVYDFIDRADLWTDRLPHVRAVRLEEPEPGL
                     QTLEMDTLSKDGTSHTTKSYRVCLPHHRIAYKQVTLPALITLHTGRWTVTPGANGVTAS
                     SQHTFTVDPGKVTKILGADATVQD"
     gene            15120..16028
                     /gene="juiF"
     CDS             15120..16028
                     /codon_start=1
                     /gene="juiF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /product="Thioesterase"
                     /protein_id="QNL10618.1"
                     /sec_met_domain="PF00561 (E-value: 1.8e-08, bitscore: 24.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 3.4e-11, bitscore:
                     34.1, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVAQPAVAGGVRGISLDADGITLSALLALPPQAPCRAVIVALHG
                     AGMSSAYFHGSAHPDTSFLGLATSLGFGVVAVDRPGYGRSARQLPEGQGVADQATTLGA
                     ALRFLAARYDTGSGLFLLAHSFGGKAALRIAADRAVPELLGLDVSGCGAEYAVPLGDRE
                     PHGRQSRRLNWGPLRLYPPGTFRASGGVVAPVPSLELADAARWPGDFAGFAGRVRVPVR
                     FTFAEHEFWWQHDARAVARLRSRLSGAPRVVTDHQPDAGHNISLGWSARAYHLRVLGFV
                     EECLRWRESRQRATPEEQEGT"
ORIGIN
        1 tcaggcggcg ccgaaggccg tacggtgctc cgccgcccag gcggcgaagg gcgtcggcgg
       61 gcggccggtg acccgggtga cggtgtccgt cagcggggcg tcggtaccga ccgaggcggt
      121 gtacgtggcg aggacgctgt cgacgagccc gggcaccccg aacgcgccgt actgccgctc
      181 cagctgggcc cgggccgcct cgccgaccag gtcggtgacg gtcaccggac gcccggtcgc
      241 cgccgcgatc agctcggcct gccggcgttg cgtcatcgac tccgggccgg tgacgtcgta
      301 cgtcgccccg tcgtggccgc cggagagcag ggcctggacg gcgaccgccg cgatctcgcg
      361 ctcgtggacc ggggagaccg tgctctcggc gtaggggatg gcgacctcgt cgccggcccg
      421 gatcgccggg gcccactgga gggtgttgct cgcgaaggca ccgggccgca gcatcgtcca
      481 gcccaggccg ctgccgcgca ccgcttcctc gaccgacagg tgcatgagcc cgatcgggtt
      541 ggcgctctcg cgcaccgcgg cggaggagag cagcaccacc tgtcgcaccc cggatgcctt
      601 ggccaggtcc aggaacccgg cgacgcccgc cggatgcggg aagaggaaga gccgctccac
      661 gcccgagagc gcgtccccga acgagccggg gtcgtccagg tccccggcgc gcacgtcgac
      721 gccggcgggc agcccggcgg tctccgggct ccgggaggtg gcccgcaccg ggacaccggc
      781 cgcgagcagc agctcgacga cgttgcggcc gacgttgccg gtggcaccgg tcaccagaaa
      841 ggtcatgacg tactcctgat cgaggggagg gggacgcgta cgcgcgtcaa gggcggggtg
      901 cgccgaacca gcggtggagc gcgtcggcga gcggggtgcc gggcacccag cagatgtggc
      961 cgtcggggcg gacgaggacg gcctcggcgc tgaggccggg cagttcccgt accgccacgg
     1021 cgtccacccg tcccgtccag ggagccgctt cggccaccag gtccggcagc gcggtcaaca
     1081 ggacgccgtg tccctggtgg aacaggtcgc cggcccagcg gccgtcgggc aggaccatgt
     1141 cggcgagccg ggagccgacg ggctcgtcgc cgccgaggtc gtagcggatg gagagcccgg
     1201 agatcgcgcc gccgagcaga cggttcgcct ccggcagcgc gagcagctgg gtgaagatct
     1261 cccgcagggc gtcgtgttcg acgcccttct ggccgagagc cacctgggcc cgggtgttgg
     1321 tcaggacggc cgcgcccacc gggtgccgtt caccgtggta ggtgtcgagc agtccttcgg
     1381 gggcccagcc ggcgacgtcg gcggccagtt tccatcccag gttcatcgcg tcctggatgc
     1441 cggtgttgag cccctgcccg ccgagcggca ggtggatgtg ggcggcgtcg cccgcgagga
     1501 aggcccgtcc ggcccggtag tgctccgcga gccgtgaggc gtcgctgaac cgagaggccc
     1561 agcgcacctc cttgacctcg gtctccgagc cgtagaactc ctcgatcgcc gccaccactt
     1621 cggtgtcggg gaccttggcg cgctgatcgt cgatcacctg gtcggcgacg ctgtagaaga
     1681 tccggtggac gcggtccgac tcgttcccga tcgggaagat gttcgcgaac gagccgtcgg
     1741 ggcgcatccg gggcgagccc ttggtgtccc aggtcgtcgc gatgccctcg ccggcgacgc
     1801 ccaggacgac gtccgagacg gtgccgaacc accgggcttc caggccgggg aacgcgatcc
     1861 cgagctgctt gcggacgaag ctgcggccac cgtcgcagcc cgccaggtag cgcgcctcga
     1921 cccacgtgcc gccggcctgg acgcggatgc gctccgcgtc ctgctcgatg tccgtgacct
     1981 cgacgttcca ccggacccgg acgcccaggc cggccaggtg ctcctccagc acctgctcca
     2041 cctcggcctg tgggatggcg acctgatagg ggtagcggga gttccagccg tcgagggcca
     2101 gcggcaccgg caggtgcgcg aagtgggtgg tttccaggcg tgctctggcc tgttcctccg
     2161 caccgtcgag catgccgcgc atggccagga tctccgcggt gcgggggtgg aggttcaggg
     2221 cccgactctc gccggtgcgg cccggcccct tgtcgaggag ggccacatcg acgcccgcca
     2281 gggctaattc gtgggccagg gtcagaccgg tgggcccggc gcccaccacg atcacgtcag
     2341 cgtccatcag gattccttcc gccttccgtc ctgaacgatg ttctatcaac gaacgtcgtt
     2401 cttcggcaag tgcaatgttc ggccccatgt ggtgcgaacg cccggagagg ccggctcacc
     2461 aggcgtgggc cggggtgatg ccccacgcgt ccagttccgc ctccaccagc ccggggccga
     2521 ccgcgacgcc ggcagactgg aaaccgacgg agaccgtcgc gccggccctg aggacgaagc
     2581 agttcatccg gccgggcggc gctccggcgc cggacaccgt gcggaagagg acccccagag
     2641 cggggccgaa gacgtcacgg gcctccggca gcatctcccc ggcgttggac agcgccgcgt
     2701 cggtctgcgg atagccggtg aggtgccggc cggccgcctc ggcgtacacc gccttgctca
     2761 ggcggcggat ctccacgagg tcggtgtagc gcccccgcag accgtccagc cgcagcgtgg
     2821 cgatcccgac catgttggag gactccaggt cgtcggggcg ccggaaactc accggcagcc
     2881 cgaccgtgaa cggccggccg gcgtcggtgg tcccccgcgc agcgcgcagg atgttcgcgg
     2941 tcatggcgag catcagcccc gtcacgctgc ccccgccgtt ccgcgccacc cggtcggcct
     3001 cggcggccgg cacggtgaag gcgagccggg agcggaccgg cgccgcgccc ccgccctgct
     3061 cggtacgccc gagcagtacc cgggcggcgg tggccggcac cgaggccgcc ttcacgcgca
     3121 gccgggacag cgccagccgg gagtacgtcc acagaccgtc cgtcaccaca tcggtgaggt
     3181 cgtcgaccag cgagggctgc ccggagtgcc actgccgggc ggtccgccgg gcggcgagcg
     3241 tggccgcgcc gatgagaccc tggccgtcgg tcagggcgtg cgatccgacc agggacacca
     3301 cgaaggtgcc gtcggagacc ggtgccgcgc tcagttccca gccgcggccc gcgtgggcgt
     3361 ccagctcggc tcggcaggcg gcgtcggccc agtcgagcac cgtctccggg gcgactgccc
     3421 gggaggagac acgcagcggc gcgcagccgg tgtgcccctc ccagaccggg cgggccaggg
     3481 ggagccgggc cgtgcgcatc cggcggttca acgggccctc ggccaggctg gtgtgcaagg
     3541 tccgcagcgc ctcgtggtcc ggccggcccg gcaccctcca gagcaactgg accggggggt
     3601 cgacgcgcag ctgccgcatc aggacatcgc ctccggtgaa gcgctgtgtt ccggccgcgg
     3661 tcatttggcc gcccccgtac cggtgagccc gaccagcggg gccagggtgc gcccggccag
     3721 cgcgatggcg tccggatcgt gcgcgttggc caacatgttg gccatgagcc cgtccacgcc
     3781 gtggcacagc acccgctcct ggagttgccc ggtcacctcg gccggggtgc cgacgaagaa
     3841 cctgtcggtc gccgcggccc gctcggaatt ggtcaggccc gtcacgtcca cgcccgatct
     3901 ggccaggacc tcctccagca gcttgcgggc ctggtcgccg tcctcgtgga tgatcagggg
     3961 cgtggcgaag ctggtctcca gcatcgccgc gtcccgcccg gcctcggcgc agcgcgtccg
     4021 taccgcggcc attttgcggg ggagttccga ggtggtgcag atgatgttga ggtggtccgc
     4081 gtgctcggcg gcgaaggcga acgtcttctt ctctccaccg cctccgatca gcaccggcag
     4141 gtcgtcgcgg agccggggtt cgttgacggc gtcctcggcg cggaaccagc gcccctcgac
     4201 ctgcggccgt gacccgcgca gcatcgggac ggcgatgccg agtgcctcct cgaaccggct
     4261 gaaacgctcg gcgaccgtac cgaacacgta gccgtagtca tggtgttcac gctcgtgcca
     4321 gccggccccg acgcccagca cggcgcgtcc gcggctgatc acgtcgagcg tggtgaccat
     4381 cttggccagc agggcggggt tgcggtagcc gatgccggtc accatggtgg acagccgcac
     4441 ccggtcggtg accgccgcca acgccccgag cagggtgtac gcctccagca tcgggccctc
     4501 gggcggcccg atcagcggtg gttggtagaa gtggtccatc gtcgtgacca ggtcgaaccc
     4561 ggaggcttcc gcggtccgca cctgggcgac gagagtgggg aacagttcgg cggtccgcgt
     4621 gtgatggaag tacgggatgt ggtacccgag acgtatgctc acgtgttggt cctcctacca
     4681 ggtgacggga agttccacgg ggccgaagaa ctggccgaag tacttgtagg gcagggcctc
     4741 cggcgccgtg gcgagccgca ggtccgggat gcggtggaag aggcggtcga ggaccacctg
     4801 aagttcggca cgcacgaggt tctggccgag gcactggtgg gccccgtagc cgaaggcgac
     4861 gtgccgggtt cccgggaggc ggccgatgtc gaagcggtcg gggtcgggaa aggcgccggc
     4921 gtcccggttg gccggcaggg tggggatgag gacaccgtcg cccgcccgga tgcggacgcc
     4981 gccgatctcc acgtcctcca gggccagccg gagcggcccg gtgtcggtga tcgacaggaa
     5041 gcgcagcagc tcctcggcgg cggccggggt gcgggacggg tcggcggtca gctgcttcaa
     5101 ctgctcgggt tcggagagca gggtgagcac cgacagcgag atcatcgccg cgctggaggc
     5161 gtgtccggcg atctgtagca gctgtaccag actggccagt tcgaaacggt cggcggcatc
     5221 gccgtgctct cggcgggacc ggtcgatctg ccggctgagg aagtcgtcgc ccggattctc
     5281 ctccttggcc gcgatgacca ggtcgaggtg ggcgtggagg gcctcggcgg cggcccgctg
     5341 ctcgtcctcc gtcctggcct cgtgcagcat cttgccggtg ttgcgctcga tggcgtcgag
     5401 atcctccgcg ggaacaccga ggaacgccga gagcaccagg gtgggcacgg gctgggagag
     5461 gtgcgcggcg aggtcgacgg gcctgcccgg ctgttccagc atccggtcga tcgccgcgtc
     5521 gacgatgcgc cgtattcgcg gcctcatggc attgatcctg cgcacggaga attcaccgag
     5581 gaccgcgcgg cgggcctgcg cgtgctcggg cccgtccatg ttcacgagga acggcgagaa
     5641 ataggtcagc gccttgagtc cgcccggaga cagttccgga aatccggggc ggagtttctc
     5701 ggaactgaac cgggcgtcgg agagcaccgc acgggcgtcg tcgtgcttgg tgacgagcca
     5761 ggccgggcgc ccgtcggaca tgcggacccg gtgtatccgg ccgccggtcc cctccagttc
     5821 ccggtaggtc tccgggacgc tgaacgggca ggtgcgaagt atggggaatt cgggtacgga
     5881 ttcagctgtg atctcgctca tgacagttcc cttgggtaag ccgagggcgg atgtcgtgaa
     5941 cgctagcgcg ggcaatttgc gctgagggtc agaccgaagt cagctctttg tcaattcccg
     6001 gccccgtgca ttgcacgggg cgatgacgga acgaacggcg gccggattgc ggcgtcactc
     6061 ctcgacgaac gtcgcgagcc cggagatcag catgtccagg cccagttcga accgaccgtc
     6121 gctgtcggag tccagcatga cgtccacgtt gccggcgatg gcggggaacc ggtctgcggg
     6181 cagggacgcg aagtactcgt ggacgggctc gatccagttg tccaggccct tgtgcgccag
     6241 gtgccagcct tcgtagacgt cggccgtcgt gtagatggtg agccggtgca gagcccaggc
     6301 cgcgacccgg ggcgggacgc cgacgtccag caggaccgac agcaggcgct cggtgatggc
     6361 cacgccgatg gcggtgggcg gaaaggcggc gaagttgtag agggcgatgt caccgtgggc
     6421 cgacagcacc cgctgcatct tcgtgtagtg cgccttgagg tccgcgtccc actgcccggt
     6481 gagctccggg acacccacct cccgcatcac gccgtcgagc accaggagca gcagttcctc
     6541 gcggttgcgg acatgtgcgt agaggctgga cgcgaccacg ccgaggtctc cggcgatccg
     6601 gcgcatggtg atcgcggagg tgccttccgt gtcgacgacc gcgagcgcgg cgtcgacgat
     6661 ccgctcagcg gtgagcgggg cgcgcacgcg agtcccggac ttccggcgta cctcccacgg
     6721 agggacgggc acgtcggcgg agttcgactt ctggcgcatc gcggagacct tgcgggtgag
     6781 ggcgcccggg ggcgcggcgg agacagattc gggttgacgc cgaacaccat tctgccgtag
     6841 aacggtgttc acttgacaag ttgatgttcg gtggtgggca acattgttca cgcatcgtac
     6901 gacgtccaga gcgtacgcgg caccgggctg tcggcggcac cgtcacaccg ttccgaccca
     6961 ggaggtcacc atgtccacca ccgaaacccc gcgtgcggcg gccttaccgg aaggcccccc
     7021 ggtctccgcc cgccgggcct gggcggtcgt cggcatcatc ctggtgggcg agatcatgga
     7081 cctgctggac gctctggtca cgaccgtcgc cgggccgacg atcatgcggg acatgggcgg
     7141 ctcgcaggag ttcatccagt ggctcgcggc cgggtatacc atcgccatgg ccgtcggact
     7201 gctgatcggc gggcggctcg gtgacaagta cgggcgcaag aacctcttcc tgatcggcct
     7261 gaccggcttc accctgacct cgcttgtctc ggcgctggcg accagcccgg agaccctcat
     7321 cacggcccgg gtcctccagg ggctgctggg ggcgctgatg gtcccccagg cgctcggcat
     7381 catccggagc gtcttcccgc ccgacaaggt cggtctggcc ttcggtgtga ccgggccggt
     7441 gatggcgctg tccggggtgg ccgggccgat cctggcgggc tggctggtcg acgcggacta
     7501 cttcggctgg ggctggcgga tgatcttcgc tgtcaacatt ccggtcggtg tgtccgccct
     7561 ggtggccggc cttgtcctgc tgccccgctc gcagcggcaa cgcgacctca agatcgacct
     7621 ggtgggtgcg gtgatggccg cggcgggcat gggcgccgtg attttctcgc tcgtccgggg
     7681 ccgcgagctg ggctggccgt ggtgggtggg ggccctgctc ctcgcgggcg tcgtcgctct
     7741 cgccggcttc gtccggtacc agttccaccg ggcgggccgg ggccttccgg cgctcgtcgt
     7801 tcccacgctg ttccggaaga aggcgttcgt ctccgggttg ttcgtcggca tgctgttctt
     7861 cagcgtgatg atgggcggcg ccctgctgtt cagcctcttc tttcagctcg ggctcggcat
     7921 gagcccgttg atggcgggtc tcgccacgat cgcgcaggcg gtgggaatgc ttttcgggtt
     7981 cgccttctcc caggcgctcg gaatggcgcg gcgcaccatg tacatcgggt tcgcggtctc
     8041 cgcagccggc ctgctgggtg cgctggggac gatcgccgtg cagagcgatc cggtgaacgc
     8101 ctggtggctc tcgccggcgc tggcggtgat cggcatcggc tccgggctcg ccatcgcccc
     8161 gttcttcgac atcacgctct cgggcgtcga cgaggcggag accggttcgg cctcgggcac
     8221 cctgacctcc gcccagcagg tgggaaacgc gctcggcgcc tcggtactcg gcacggtctt
     8281 cttttccgtg ctgaacgcgc accgcgaaga cggggcgccg gccttcacca cggcgacctc
     8341 gacggctctc ctcgtggccc tggcgctggt cgcgggcgcc gctctgtgca cccgagggct
     8401 gcccgagcgg gcggtgcggc atacggaggt gtgacggccc gtacaaccga gggaaacact
     8461 ccggtaattt ctcgccgccc aggcgtcgga cgcgttggat cacgccagac cgaaaaaatc
     8521 cagcccatcg tttcggggcc cctccgggcc ggaccccgac gtgttagcgt gaccggcgga
     8581 tttttagctc cgacgcctcg aagggctgaa aagagaaaaa gaatcagggc cgatgagggt
     8641 gtgcacctga taagccgcga gcggccgtgt gcgccgtcgc gaacctcgcc cggtcaatac
     8701 cgttctttca cagaagaagg tcaggatgtc tttcgttgca catccgcttt cacgggggaa
     8761 aatgaatgga acagcatgct gagacaccgg agattgacag atgggcggtc gcggccggcg
     8821 ggattgacca acagcggcgc ggaggcagga cggcgacgcg gcctctccct gtctcctcac
     8881 ccgtcttcga cacctggcag gtcctactcg tggagagcga gcagcaccag cggggagtgc
     8941 tcagccaggc actgaaccgg cacgtgcacc aggtgacctg tgtcgacacc ggcgccgagg
     9001 ctctcgacgt gtacgaccag gcggacgtgg tgctgctgaa ccccaacctg cccgacatcg
     9061 acgggctgga ggtgtgccgg gcgatccagg ccacctgcgg aatcccgatc atcgtgatca
     9121 ccgaccgctc gtcggagctg gactgcgtcc tggctctcca ggccggcgcg gacgactacg
     9181 tggtccggcc gtacgggctc cgggaactca tggcccggat ggacgcggtg atgcggcgcg
     9241 catccccgcg ccggcagagc gagcacgtca tcgaacacgg cccgttgcgg atcgacacgc
     9301 acacccgcga agtccggttg tgcgaccgga cgatcccgat gagccgcaag gagttcgacc
     9361 tgctccacct cctggcggtc aaccgccaca gcgtcgtccc acgcgaacgc atcatgcgtc
     9421 aggtgtggga gaactcctgg tcgcgccgga ccgtcgacac gcacgtcagc agcatccgcc
     9481 acaaactcgg cggaaattcc tggattctca ccgtgcgggg cgtgggattc cgcatagggc
     9541 agggcgcacc ggccgtcccg taggaaaagg gcggtctccg ccggccgtcg ccgcagctct
     9601 cccgcgctgt ggcgacggct ttttactgcc ctgagctggc cgtctgacct tcctctgaca
     9661 aagtgctgaa tctgccgtaa gaagacctga ccgtttccgc catcggaaat gtagaagtcg
     9721 tgaactggat cgggttgagg gtgcggcgag cgctggtgaa tagttgttcg tgtggtccgg
     9781 aacagcggaa cggaagcgga ggaagggtat gttcacagcg gtgagcgtgg gcagactgga
     9841 aagggatgtt cgcgatgaga tatccgagat cttcgccgat tttgatacgg ccaccgagca
     9901 cggggcgtcc ggcctgaggc gtcggcagct gtacgcctac tacgacatct tcatccacct
     9961 tcaggacttc accggggaga acgccgccgc ggccgcccgg gatgcggtgg agggccccgg
    10021 gacggcgcgg gtccgggagc ggctggcccg gctggttccg gcctacggcc cggccgggga
    10081 cgaagaccgc cccacggcct tctaccactg ggagggcgaa cctccggtgg acaaggagac
    10141 cgggttgcac accacggtga tcgtgaaccg ggtcgccgac gaggtcattc ccgaggtggc
    10201 ccggcttttc ggtggactgg atgccaccgg cttcccgcac cggatgggta cccggcgccg
    10261 gcagctgttc gcgtaccagg gcgtgtactt ccaccttcag gacttccccg gcgaggacgg
    10321 cacggccgtc atcggacagg catggcggga ggccgatccg cgcttcgtgc agatctgcgc
    10381 ggacctggac ccgctggtga agaagtacgc cccggagcgc tggcggtcca cggccgacca
    10441 gatcgccacc cgcttctacc actgggaggc ggcggagtga ccgcgcgacg tgtcgtcatc
    10501 accggcgtcg aggtgatcgc tcccggcggc gtcggctccg ggaagttctg ggagttgctg
    10561 accgacgggc gtaccgcgac ccgggacatc accttcttcg acccgtcccc gttccggtcc
    10621 cgcgtggcgg ccgaggtcga cctcgacccg tatgcgcacg gcctgacccc gcaggaggtc
    10681 aggcggatgg accgggccgc ccagttcgcc gtggtcgccg cgcgcggagc cgtcgccgac
    10741 agcggcctgg ccctggacgc gctcgacccc taccggaccg gcgtcaccgt cggtaccgct
    10801 gtcggttcca ccatgagcct tgaccaggac taccggatcg tcagcaacga cggccggctc
    10861 gatctggtcg accacaccta tgccgacccg cacttctacc agtacttcgt gccgagttcc
    10921 ttcgcggccg aggtcgcccg ggacgtcggt gcccagggcc cgagcggcgt cgtgtcggcc
    10981 ggctgcacct ccgggctcga ctcggtcggc tacgcggccg agctgatccg cgagggcacg
    11041 gtcgatgtcg tggtcgccgg tgccaccgac gcgccgatct caccgatcac cctcgcctgc
    11101 ttcgacgcca tcaaggccac cacaccgcgc aacgacgatc cggagcatgc ctcccggccc
    11161 ttcgacggca cccgcaacgg gttcgtcctc ggcgagggtg ccgcgatgtt cgtcctggag
    11221 gagcgcgaga gcgcgcgccg gcggggcgcg cggatctacg cggagatcgc gggatacgcg
    11281 acccggagca acgcgtacca catgacgggc ctgcgggccg acggcgcgga gatggccgag
    11341 gcgatcaccg ttgctctcga cgaagcccgg atgaacccca cggagatcga ctacatcaac
    11401 gcgcacggct cggggacccg gcagaacgac cgccacgaga cggcggcgtt caagcggagc
    11461 ctgggcgacc acgcgtaccg cacgccggtc agctccatca agtccatggt gggccattcg
    11521 ctgggggcga tcggctccat cgagatcgcc gcgtcggtcc tggcgatccg gaacaacgtc
    11581 gtcccgccca ccgccaatct gcacactccc gacccggagt gcgacctgga ctacgtgcca
    11641 ctcaccgccc gtgaccaact caccgacgcc gtcctcacgg tcggcagcgg cttcggcggg
    11701 ttccagagcg ccatggtgct ggcccagccc gaaaggagca tggcgtgagt gcctcggtgg
    11761 tggtgaccgg tctgggcgtg gcggcgccca acgggatcgg tgaggacttc tggacggcca
    11821 ccctggccgg caagagcggc atcggccggc tcacccggtt cgacgcctcc cggtacccgg
    11881 cccggctcgc cggggagatc gacgacctca cggcggagga gcacctcccg agccggctga
    11941 tcccgcagac cgaccgggtc acccgactct ccctggtcgc ggcggaccgg gcgctcgccg
    12001 acgcgagggt gcgtcccgca gaactgcccg agttcagcat gggcgtgagc acggcgagtt
    12061 cggccggcgg gttcgcgttc agccagaacg agctgcggaa cctctggagc aagggcagcc
    12121 agtacgtcag cgcctaccag tcgttcgcct ggttctacgc ggtcaacacc ggccagatct
    12181 ccatccggca cggtatgaaa ggccccagcg gggtggtggt cacggaccag gccggcgggc
    12241 tggacgcggt cgcgcaggcc cggcgccaga ttcgcaaggg cacctccctc gtcgtcgccg
    12301 gcgccgtgga cgccttgctc tgcccctggg gatgggtggg actgcacacc agcggccgcc
    12361 tcagcacagc cgaggagccg gcccgcgcct atctgccctt cgacgaccgg gccgccggcc
    12421 atgtcgctgg tgagggcggc gccctgctca tcgtggagtc gggtgcctcc gcacgcgccc
    12481 gcggagccga ggtctacggc gagatcgccg ggtacgccgc gaccttcgac ggacgcgagc
    12541 ccggcctgcg caaggccgtg gaactggccc tcgcggacgc gggggcgagc ccctccgacg
    12601 tggacgtggt cttcgccgac gcggccgccg agccggagct ggaccgggtc gaggccgagg
    12661 cgatcaccaa ggtcttcgga gcgtacgggg tgccggtcac cgcgccgaag acgatgacgg
    12721 gacggctcta cgccggcgga gcggcgcttg acctcgccgc ggcgttcctc gccctgcgcg
    12781 agtcgacgat cccgccgacc ctccacaccg aactcgccga acggtacggc ctcgacctgg
    12841 tcgtctccca gccgcgtccc gcccggctgc gtaccgcgct cgtcctcgcc cggggccacg
    12901 gcggtttcaa ctcggcgatg ctcgtgcgct cctgagcgtc cccgtgctcc cagcccggcc
    12961 ccgggacgac cgcaccaccc accatcacga accgaaccca ccatcaggaa ccgaaggaag
    13021 gcttcccatg tcccgcacct tcacgctcga cgacctcaag cgcatcctcc tcgaaggctc
    13081 cggtgccccc gacggagccg acctcgacgg cgacatcctc gacaccgagt tcaccgacat
    13141 cggctacgac tcgctcgccc tgctggagac caccagccgc gtcgagcgcg agtacggcat
    13201 cgagctggac gagtccctcg tgggtgacgc cagcacgccc cgcgccctcc tggacgccgt
    13261 ctccgcccat ctgcaggccc agcccgcctg agcccgttgc ccgccgggcg aggacccggc
    13321 tccggcccgt cgtgaaacac gttgaggaag agggaacatg acagcacagc agagccgcgt
    13381 cgtcctggtg accggtgcca ccagcggcat cggtctgtcc gtcacccggc tgctggccgg
    13441 acagggccac cgggtgtaca tcgggtcccg tgccgaggac aacgtcgtca gaaccgtcaa
    13501 gcaactccag gaggaggggc tcgacgtgga cggcaccgtc ctggacgtgc ggtccgggga
    13561 ctcggtccgc ggtttcgtcc aggcggccgt cgagcggtac ggcaccgtcg acgtgctcgt
    13621 caacaacgcg gggatacccg gcggcggcgt cacggccgac gtctccgacg cgctgtggga
    13681 cgacatcatc gacaccaact tgaacagcgt tttccgcatg acccgagagg tgctgaccac
    13741 ggggggcctg cgtcacaaag agtggggccg catcatcaac atcgcctcca ccgccggcaa
    13801 gcagggagtc gtcctcggcg ccccctactc cgcctccaag cacggcgtcg tcggcttcac
    13861 caaggccctc ggcaacgagc tggcgcccac cggcatcacc gtcaacgccg tgtgcccggg
    13921 gtacgtggaa accccgatgg ccgagcaggt ccggcggaac tactcccgca tctccggtgc
    13981 cccggagtcg gccatcctgg agaagttcca ggccaagatc ccgctgggcc gctactcgac
    14041 ccccgaggaa gtggccggcc tggccggcta cctggtcacc gacgcggcgg cctccatcac
    14101 cgcacaggcg ctcaacgtct gtggcggcct cggcaacttc tgaccattca ccgagcgcga
    14161 aggagtcacc ccatggcgtc gaagccgcac acggcccgtc acgagatcaa gatctccgcc
    14221 cccgccgaga ccgtctaccg gatgctcgcc caggccgagg aatggccccg gctcttcccg
    14281 cccaacatct acgtcgccca cgtcgagcgc ggccagagcg aggagcgcat ccgcatctgg
    14341 gccacggccg gcgactccgt gaagaactgg acttcgcacc gggtcctcga cgcggagaac
    14401 ctcagtatcc ggttccggca ggaagtcagc accccgccgg tggccgcgat gggcggcacc
    14461 tggcacgtcg aggcgctggc cgccggagac acgctggtcc gcctcgacca cgactaccag
    14521 gcggtcgacg aggaggggct cggctggatc gacgaggtcg tggaccgcaa ctcccgcgcc
    14581 gagctggaat cgctcaagac gatcgccgag tcggacggtg cggtcgccgg actcagctgc
    14641 tccttcgagg acacggtcgc catcggcggc agcgcccagg acgtgtacga cttcatcgac
    14701 cgggcggacc tgtggaccga ccgcctgccg catgtccgcg cggtacgtct ggaggagccg
    14761 gaaccgggcc tccagacgct ggagatggac accctgtcca aggacggcac ctcgcacacc
    14821 acgaagtcgt accgtgtctg cctgccgcac caccggatcg cctacaagca ggtcaccctg
    14881 cccgcgctga tcacgctgca caccggtcgc tggaccgtca cgccgggcgc gaacggagtg
    14941 acggcctcct cgcagcacac gttcacggtc gatccgggga aggtcacgaa gatcctgggc
    15001 gcggacgcga ccgtgcagga cgccatcgac tacgtccgca cggcgctcag caccaacagc
    15061 atggcgaccc tgggcctggc caaggcgttt gccgaggggc gggccggggg cgaccgtcga
    15121 tgacggtggc ccagccggcc gtcgcggggg gagtgcgtgg catctccctc gacgcggacg
    15181 gaatcaccct gtccgcgttg ctcgccctcc cgccgcaggc tccgtgcagg gcggtgatcg
    15241 tcgccctgca cggagccggg atgagctccg cctacttcca cggttcggcg cacccggaca
    15301 cctcctttct cggccttgcc acgagcctgg gattcggcgt ggtggcggtg gaccgtccgg
    15361 gctacggccg gtcggcccgg cagctgcccg aggggcaggg ggtcgcggac caggcgacga
    15421 cgctcggcgc cgccctgcgg tttctggccg cccgctacga cacgggcagc ggcctctttc
    15481 tcctcgccca ctcgttcggc ggcaaggcgg cactgcggat tgccgcggac agagccgtcc
    15541 cggagctgct cgggctcgac gtctcgggct gcggcgccga gtacgcggtc cccctcggcg
    15601 accgcgagcc gcacggccgg cagagcaggc ggctgaactg ggggccgctg cggctgtacc
    15661 caccgggcac cttccgggcc agcggaggcg tcgtcgcccc ggtcccgtcc cttgagctag
    15721 cggacgccgc ccgttggcct ggcgacttcg ccggcttcgc ggggcgggtc cgggtgccgg
    15781 tccggttcac cttcgccgag cacgagttct ggtggcagca cgacgcgcgg gcggtcgccc
    15841 ggctgcggag ccggctctcg ggtgcgccgc gcgtcgtgac ggaccaccag ccggacgcgg
    15901 gtcacaacat cagcctcggc tggtcggccc gcgcctatca cctcagggtc ctcggcttcg
    15961 tggaggagtg cctgcggtgg cgagagtcga gacagcgagc cacgccagaa gagcaggagg
    16021 ggacatga
//
