BGC0002517 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002517 (Streptomyces sp)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	73,855	tripartilactam/niizalactam C	PKS	100%

BGC0002517 - Region 1 - T1PKS

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Location: 1 - 73,855 nt. (total: 73,855 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 73,856
Label		BGC0002517

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002517	1.00	Polyketide	tripartilactam, niizalactam C	Streptomyces sp.
BGC0001770	0.49	Polyketide	sceliphrolactam	Streptomyces sp. SD85
BGC0000016	0.40	Other	amphotericin B	Streptomyces nodosus
BGC0002404	0.35	Other	falcarindiol	Solanum lycopersicum
BGC0001163	0.35	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001164	0.34	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000867	0.33	Other	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0002631	0.33	RiPP	myxarylin	Pyxidicoccus fallax
BGC0000122	0.33	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0002000	0.31	Polyketide	setomimycin	Streptomyces aurantiacus JA 4570

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002517	1.00	Polyketide	tripartilactam, niizalactam C	Streptomyces sp.
BGC0001770	0.78	Polyketide	sceliphrolactam	Streptomyces sp. SD85
BGC0000016	0.72	Other	amphotericin B	Streptomyces nodosus
BGC0002404	0.68	Other	falcarindiol	Solanum lycopersicum
BGC0001163	0.68	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001164	0.67	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000867	0.66	Other	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0002631	0.66	RiPP	myxarylin	Pyxidicoccus fallax
BGC0000122	0.66	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0002000	0.65	Polyketide	setomimycin	Streptomyces aurantiacus JA 4570

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 73855:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccmal - ccmal) + (Me-ccmal - ccmal) + (ccmal) + (ohmal) + (ccmal - Me-ccmal - ccmal) + (mal)
Non-colinear ordering:: triA1 + triA2 + triA3 + triA4 + triA5 + triA6

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

triA2

PKS_AT (562..857): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 222.1
Ethylmalonyl-CoA: 210.9
Methoxymalonyl-CoA: 186.5
2-Methylbutyryl-CoA: 141.8
trans-1,2-CPDA: 136.3
Malonyl-CoA: 105.7
Propionyl-CoA: 103.9
CHC-CoA: 102.8
Isobutyryl-CoA: 85.2
Benzoyl-CoA: 66.6
3-Methylbutyryl-CoA: 53.5
Acetyl-CoA: 50.0
fatty_acid: 35.7
inactive: 29.6

PKS_AT (2257..2552): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.4
inactive: 109.8
Methoxymalonyl-CoA: 56.1
Methylmalonyl-CoA: 41.6
Propionyl-CoA: 33.1
Isobutyryl-CoA: 28.4
Acetyl-CoA: 21.0
Benzoyl-CoA: 18.1
Ethylmalonyl-CoA: 8.3
3-Methylbutyryl-CoA: 6.4
2-Methylbutyryl-CoA: 3.4
trans-1,2-CPDA: 2.7
CHC-CoA: 2.0
fatty_acid: 0.0

triA1

PKS_AT (664..958): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.2
inactive: 101.8
Methoxymalonyl-CoA: 73.9
Methylmalonyl-CoA: 66.4
Propionyl-CoA: 36.2
Isobutyryl-CoA: 35.4
Ethylmalonyl-CoA: 27.2
CHC-CoA: 25.6
Benzoyl-CoA: 16.9
fatty_acid: 16.3
2-Methylbutyryl-CoA: 15.8
Acetyl-CoA: 13.4
trans-1,2-CPDA: 4.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (2346..2633): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 190.0
inactive: 100.1
Methoxymalonyl-CoA: 65.8
Methylmalonyl-CoA: 54.9
Isobutyryl-CoA: 30.8
Propionyl-CoA: 25.8
Ethylmalonyl-CoA: 22.6
Acetyl-CoA: 19.1
Benzoyl-CoA: 15.3
fatty_acid: 12.0
2-Methylbutyryl-CoA: 4.3
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

triF

AMP-binding (20..415): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DYYPTTQRVK
Nearest Stachelhaus code(s):: 3S-Abu DYYPTTQRIK (91% 8Å match); Asp DYYPTTMRVK (68% 8Å match)
Stachelhaus code match:: 90% (moderate)

triG

AMP-binding (12..410): X

nrpys: bMe-Asp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DCFFFALITK
Nearest Stachelhaus code(s):: bMe-Asp DCFFFALITK (94% 8Å match); Ala DCFFFALITK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

triI

PKS_AT (31..287): pk

ATSignature: (unknown)

No matches above 50%

minowa: CHC-CoA

Prediction, score:

CHC-CoA: 12.0
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

triA6

PKS_AT (550..827): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.6
inactive: 100.3
Methoxymalonyl-CoA: 70.3
Methylmalonyl-CoA: 65.9
Isobutyryl-CoA: 35.5
Propionyl-CoA: 28.5
Benzoyl-CoA: 15.2
Acetyl-CoA: 14.9
fatty_acid: 12.9
CHC-CoA: 9.2
2-Methylbutyryl-CoA: 7.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -3.4

triA5

PKS_AT (559..842): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.0
inactive: 111.8
Methoxymalonyl-CoA: 59.6
Methylmalonyl-CoA: 52.0
Propionyl-CoA: 20.7
Acetyl-CoA: 20.3
Isobutyryl-CoA: 16.9
Benzoyl-CoA: 16.4
Ethylmalonyl-CoA: 16.2
CHC-CoA: 9.1
2-Methylbutyryl-CoA: 4.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2227..2516): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.4
Ethylmalonyl-CoA: 208.8
Methoxymalonyl-CoA: 176.1
trans-1,2-CPDA: 137.0
2-Methylbutyryl-CoA: 136.1
Propionyl-CoA: 113.1
Malonyl-CoA: 102.7
CHC-CoA: 93.7
Isobutyryl-CoA: 76.5
Benzoyl-CoA: 71.3
Acetyl-CoA: 44.6
fatty_acid: 33.3
inactive: 28.5
3-Methylbutyryl-CoA: 26.2

PKS_AT (3899..4183): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 194.9
inactive: 89.6
Methoxymalonyl-CoA: 59.7
Methylmalonyl-CoA: 53.0
Propionyl-CoA: 31.9
Acetyl-CoA: 24.5
CHC-CoA: 20.5
Ethylmalonyl-CoA: 18.6
Isobutyryl-CoA: 18.2
Benzoyl-CoA: 14.3
fatty_acid: 13.1
2-Methylbutyryl-CoA: 9.3
trans-1,2-CPDA: 8.9
3-Methylbutyryl-CoA: 0.0

triA4

PKS_AT (549..826): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.7
inactive: 117.4
Methoxymalonyl-CoA: 59.6
Methylmalonyl-CoA: 47.1
Isobutyryl-CoA: 31.6
Propionyl-CoA: 22.3
Ethylmalonyl-CoA: 18.2
Acetyl-CoA: 18.1
Benzoyl-CoA: 17.0
fatty_acid: 10.7
CHC-CoA: 5.8
2-Methylbutyryl-CoA: 2.4
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

triA3

PKS_AT (569..854): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 191.8
inactive: 94.5
Methoxymalonyl-CoA: 65.6
Methylmalonyl-CoA: 59.1
Propionyl-CoA: 31.1
Isobutyryl-CoA: 28.9
Ethylmalonyl-CoA: 25.7
Benzoyl-CoA: 20.0
fatty_acid: 14.6
CHC-CoA: 13.1
Acetyl-CoA: 12.0
trans-1,2-CPDA: 9.3
2-Methylbutyryl-CoA: 7.8
3-Methylbutyryl-CoA: 5.3