BGC0002526 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002526 (Streptomyces sp)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	115,748	High	K-41A	PKS

BGC0002526 - Region 1 - T1PKS

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Location: 1 - 115,748 nt. (total: 115,748 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 115,749
Label		BGC0002526

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

pak11: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

pak14: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

pak18: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

pak21: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

pak23: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

pak27: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

pak35: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

pak5: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acylase aminotransferase glycosyltransferase methyltransferase transferase

pak10: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

pak13: methyltransferase

smcogs: SMCOG1089: methyltransferase

pak15: methyltransferase

smcogs: SMCOG1089: methyltransferase

pak16: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

pak20: methyltransferase

smcogs: SMCOG1089: methyltransferase

pak33: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

pak37: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

pak38: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: esterase hydrolase

pak12: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

pak2: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

pak32: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

pak36: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

pak28: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pak30: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

pak6: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pak7: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pak8: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pak9: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002526.3	1.00	PKS	K-41A	Streptomyces sp.
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.68	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001244.3	0.67	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.66	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002526.3	1.00	PKS	K-41A	Streptomyces sp.
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.68	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001244.3	0.67	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.66	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 115748:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Me-ccmal - Me-redmal) + (Me-ohmal) + (Me-redmal) + (ccmal - redmal) + (ccmal) + (redmal) + (ohmal - ohmal - Me-ohmal) + (Me-ccmal) + (mal)
Non-colinear ordering:: pak4 + pak9 + pak10 + pak5 + pak6 + pak7 + pak8 + pak30 + pak29 + pak28

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

pak4: mal - mmal

PKS_AT (546..849): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 174.1
inactive: 79.0
Methoxymalonyl-CoA: 56.2
Methylmalonyl-CoA: 46.6
Ethylmalonyl-CoA: 17.5
Isobutyryl-CoA: 16.9
Propionyl-CoA: 14.6
Acetyl-CoA: 14.3
fatty_acid: 11.6
Benzoyl-CoA: 10.6
CHC-CoA: 8.2
2-Methylbutyryl-CoA: 5.8
3-Methylbutyryl-CoA: 4.5
trans-1,2-CPDA: -0.2

PKS_AT (1581..1875): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.3
Ethylmalonyl-CoA: 195.4
Methoxymalonyl-CoA: 162.5
trans-1,2-CPDA: 128.6
2-Methylbutyryl-CoA: 128.6
Propionyl-CoA: 117.5
Malonyl-CoA: 105.8
CHC-CoA: 100.2
Isobutyryl-CoA: 65.8
Acetyl-CoA: 53.3
Benzoyl-CoA: 46.5
fatty_acid: 41.8
3-Methylbutyryl-CoA: 27.8
inactive: 26.1

pak5: mmal

PKS_AT (562..856): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.9
Ethylmalonyl-CoA: 200.1
Methoxymalonyl-CoA: 175.8
2-Methylbutyryl-CoA: 132.3
trans-1,2-CPDA: 131.8
Propionyl-CoA: 112.5
Malonyl-CoA: 93.4
CHC-CoA: 87.3
Isobutyryl-CoA: 60.2
Benzoyl-CoA: 50.5
Acetyl-CoA: 41.2
fatty_acid: 39.9
inactive: 21.2
3-Methylbutyryl-CoA: 14.5

pak6: mal - mal

PKS_AT (569..856): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.3
inactive: 121.9
Methoxymalonyl-CoA: 71.8
Methylmalonyl-CoA: 56.9
Propionyl-CoA: 26.1
Benzoyl-CoA: 22.0
Acetyl-CoA: 21.8
Ethylmalonyl-CoA: 20.7
Isobutyryl-CoA: 18.0
CHC-CoA: 9.2
2-Methylbutyryl-CoA: 6.0
trans-1,2-CPDA: 2.8
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2384..2661): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 182.5
inactive: 68.0
Methoxymalonyl-CoA: 62.5
Methylmalonyl-CoA: 53.9
Isobutyryl-CoA: 40.8
fatty_acid: 35.8
Propionyl-CoA: 30.8
Acetyl-CoA: 22.0
Ethylmalonyl-CoA: 14.2
CHC-CoA: 13.2
2-Methylbutyryl-CoA: 10.0
trans-1,2-CPDA: 7.7
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

pak8: mal - mal

PKS_AT (2..195): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 134.1
Methoxymalonyl-CoA: 51.9
inactive: 50.8
Methylmalonyl-CoA: 42.1
Propionyl-CoA: 28.0
Isobutyryl-CoA: 27.7
Acetyl-CoA: 15.7
Ethylmalonyl-CoA: 8.8
2-Methylbutyryl-CoA: 4.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1683..1961): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 192.1
Methoxymalonyl-CoA: 70.4
inactive: 69.9
Methylmalonyl-CoA: 59.2
Isobutyryl-CoA: 36.1
fatty_acid: 34.2
Propionyl-CoA: 32.8
Acetyl-CoA: 25.0
Ethylmalonyl-CoA: 18.9
CHC-CoA: 14.2
trans-1,2-CPDA: 8.9
2-Methylbutyryl-CoA: 4.4
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

pak9: mmal - mmal

PKS_AT (625..923): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.2
Ethylmalonyl-CoA: 191.9
Methoxymalonyl-CoA: 166.1
trans-1,2-CPDA: 137.2
2-Methylbutyryl-CoA: 135.9
Malonyl-CoA: 110.1
Propionyl-CoA: 107.6
CHC-CoA: 94.7
Isobutyryl-CoA: 80.7
Benzoyl-CoA: 61.9
Acetyl-CoA: 51.0
fatty_acid: 45.8
inactive: 27.6
3-Methylbutyryl-CoA: 26.2

PKS_AT (2465..2759): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.6
Ethylmalonyl-CoA: 195.9
Methoxymalonyl-CoA: 165.3
2-Methylbutyryl-CoA: 132.5
trans-1,2-CPDA: 127.3
Propionyl-CoA: 120.3
Malonyl-CoA: 105.7
CHC-CoA: 102.1
Isobutyryl-CoA: 70.0
Acetyl-CoA: 56.8
Benzoyl-CoA: 50.8
fatty_acid: 41.8
3-Methylbutyryl-CoA: 31.7
inactive: 26.8

pak10: mmal

PKS_AT (582..877): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.9
Ethylmalonyl-CoA: 193.5
Methoxymalonyl-CoA: 164.7
2-Methylbutyryl-CoA: 127.3
trans-1,2-CPDA: 125.0
Propionyl-CoA: 108.1
Malonyl-CoA: 103.0
CHC-CoA: 93.2
Isobutyryl-CoA: 70.6
Acetyl-CoA: 54.4
Benzoyl-CoA: 51.0
3-Methylbutyryl-CoA: 32.5
fatty_acid: 29.2
inactive: 28.4

pak28: mal

PKS_AT (603..898): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 170.9
inactive: 61.7
Methoxymalonyl-CoA: 58.9
Methylmalonyl-CoA: 47.5
Isobutyryl-CoA: 36.1
fatty_acid: 33.2
Propionyl-CoA: 28.5
Ethylmalonyl-CoA: 26.1
Acetyl-CoA: 25.1
trans-1,2-CPDA: 18.8
Benzoyl-CoA: 16.8
CHC-CoA: 16.4
2-Methylbutyryl-CoA: 11.9
3-Methylbutyryl-CoA: 0.0

pak29: mmal

PKS_AT (574..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 199.5
Ethylmalonyl-CoA: 195.0
Methoxymalonyl-CoA: 169.4
2-Methylbutyryl-CoA: 127.7
trans-1,2-CPDA: 123.9
Propionyl-CoA: 105.9
Malonyl-CoA: 101.9
CHC-CoA: 93.7
Isobutyryl-CoA: 63.9
Benzoyl-CoA: 48.4
fatty_acid: 45.6
Acetyl-CoA: 41.0
inactive: 24.0
3-Methylbutyryl-CoA: 22.4

pak30: mal - mal - mmal

PKS_AT (586..884): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.7
inactive: 82.7
Methoxymalonyl-CoA: 68.4
Methylmalonyl-CoA: 60.3
Isobutyryl-CoA: 39.7
Propionyl-CoA: 38.3
fatty_acid: 37.5
Acetyl-CoA: 23.6
Ethylmalonyl-CoA: 19.1
CHC-CoA: 13.6
trans-1,2-CPDA: 10.7
2-Methylbutyryl-CoA: 10.3
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2173..2450): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 194.0
inactive: 67.4
Methoxymalonyl-CoA: 65.1
Methylmalonyl-CoA: 56.2
Isobutyryl-CoA: 36.5
Propionyl-CoA: 36.3
fatty_acid: 34.7
Acetyl-CoA: 22.8
Ethylmalonyl-CoA: 13.8
CHC-CoA: 13.0
trans-1,2-CPDA: 10.0
2-Methylbutyryl-CoA: 9.1
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3718..4018): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 197.0
Ethylmalonyl-CoA: 190.5
Methoxymalonyl-CoA: 166.4
2-Methylbutyryl-CoA: 143.8
trans-1,2-CPDA: 120.2
Propionyl-CoA: 118.0
Malonyl-CoA: 96.2
CHC-CoA: 96.1
Isobutyryl-CoA: 62.6
Acetyl-CoA: 46.9
Benzoyl-CoA: 44.9
fatty_acid: 39.4
3-Methylbutyryl-CoA: 36.5
inactive: 18.7

Link to PARAS substrate predictor