LOCUS       BGC0002537             26816 bp    DNA     linear   PLN 19-MAY-2020
DEFINITION  Aspergillus sp. TJ23 DNA, biosynthetic gene cluster of emeridone F,
            complete sequence.
ACCESSION   BGC0002537
VERSION     BGC0002537
KEYWORDS    .
SOURCE      Aspergillus sp
  ORGANISM  Aspergillus sp.
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus.
REFERENCE   1
  AUTHORS   Bai,T., Matsuda,Y., Tao,H., Mori,T., Zhang,Y. and Abe,I.
  TITLE     Structural Diversification of Andiconin-Derived Natural Products by
            alpha-Ketoglutarate-Dependent Dioxygenases
  JOURNAL   Org. Lett. (2020) In press
   PUBMED   32402203
  REMARK    DOI:10.1021/acs.orglett.0c01358 Publication Status: Available-Online
            prior to print
REFERENCE   2  (bases 1 to 26816)
  AUTHORS   Matsuda,Y. and Abe,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2020) Contact:Yudai Matsuda City University of
            Hong Kong, Department of Chemistry; Tat Chee Avenue, Kowloon, Hong
            Kong
COMMENT     ##antiSMASH-Data-START##
            Version  :: 8.dev-5707d64f
            Run date :: 2025-08-15 22:04:40
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..26816
                     /db_xref="taxon:5065"
                     /mol_type="genomic DNA"
                     /organism="Aspergillus sp."
                     /strain="TJ23"
     region          1..26816
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /contig_edge="True"
                     /product="T1PKS"
                     /product="terpene"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /subregion_numbers="1"
                     /tool="antismash"
     subregion       1..26816
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002537"
                     /subregion_number="1"
                     /tool="antismash"
     cand_cluster    1..26816
                     /SMILES="CC(=O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="neighbouring"
                     /product="T1PKS"
                     /product="terpene"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..26816
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[19217:26716](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      19218..26716
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     cand_cluster    1..12501
                     /candidate_cluster_number="2"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..12501
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[1719:2501](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      1720..2501
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="2"
     gene            complement(101..982)
                     /gene="sptA"
     CDS             complement(101..982)
                     /codon_start=1
                     /gene="sptA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /product="2-oxoglutarate/Fe(II)-dependent dioxygenase SptA"
                     /protein_id="BCD52379.1"
                     /sec_met_domain="PhyH (E-value: 3e-26, bitscore: 82.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIESKNYPPIRRVNASQGSDAAYQILQEDGCVIVEQVICPNIIA
                     KISDDVNRVMDKATIGAKKGEQTHIINMHNRTIHMGDLVLTSKSYRDELLNLPFAHEVL
                     EKVFKKDSGDYWLNMGNILNMLPGAEAQRPHRDDYLYPVSQHMDPATSPDLMINITFPL
                     NEFRHDNGGTLLLPKSHTGPNADFYANAEDLPAAEMQVGDALIFTGKCVHGGGANRSDK
                     PRIGLALAAQPGYLTPRESNVNVPRDIVETMTPLAQRMIGWGTVRTKDTYGLNMLQDKD
                     FHEALGLKSKTA"
     misc_feature    101..26716
                     /note="Biosynthetic gene cluster of emeridone F"
     gene            order(1720..2222,2294..2501)
                     /gene="sptB"
     CDS             join(1720..2222,2294..2501)
                     /codon_start=1
                     /gene="sptB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: TS_Pyr4"
                     /gene_kind="biosynthetic"
                     /product="terpene cyclase SptB"
                     /protein_id="BCD52380.1"
                     /sec_met_domain="TS_Pyr4 (E-value: 2.7e-98, bitscore:
                     317.7, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQPITQIPLNFDTVVNLLGSASGIGWILNYTLMTYYSFRDKTYSM
                     SMLPLCCNIAWEFVYGVLCPSSTFVVRPVILSWLVLNCLVVYAAIKYSPNEWAHAPLVQ
                     RHLPLLFTVGIAACTGFHIALIRKFDPATAFLWSARSCQVLLSIGGLFQLLCRSSTKGG
                     SYVLWLSRFLGSICGVLKMTLMWKYGESRFPWLDDPLTAYCIALWIISDVLYGVVFYSL
                     RSKELAGAGKAKAI"
     gene            order(3013..3033,3093..3531,3587..3673,3732..3952)
                     /gene="sptC"
     CDS             join(3013..3033,3093..3531,3587..3673,3732..3952)
                     /codon_start=1
                     /gene="sptC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /product="short-chain dehydrogenase/reductase SptC"
                     /protein_id="BCD52381.1"
                     /transl_table=11
                     /translation="MGFLQDKVVIITGAAAGIGLATATAALDEGARVFGIDLAPAPTSL
                     QENPNFEFLRADLTDHDCPKRAVQACIAAFGQRIDGLLNVAGIADNYGSADSVTDEVWD
                     RCLAVNLTAPVKLMREVIPIMREAGKGSIVNTSSKAGLSGASSGVAYTASKHGLIGVTK
                     NVAWRFKTEGIRCNAICPGAVATEMGMTGDFSLWDQAAMEAMRPIHAAHMDMGIGATIK
                     AQEVAQTLLFLVSDLSRRVNGAVIPVDDAWSTI"
     gene            complement(4155..5138)
                     /gene="sptD"
     CDS             complement(4155..5138)
                     /codon_start=1
                     /gene="sptD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ubiA"
                     /gene_kind="biosynthetic-additional"
                     /product="prenyltransferase SptD"
                     /protein_id="BCD52382.1"
                     /sec_met_domain="ubiA (E-value: 2.4e-27, bitscore: 85.6,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSATTTEAKQTFLCKERLPRRYHPPSKVLSRLPTSFLPYGELLRL
                     HKPLGYILVCYPFLVAGAFSASIAPEVLEADFWPRITLLCLWSIFLRSGGCIWDDIADQ
                     HVDAKVARTRLRPLPRGAVSNSQATIFAAGTFLCGFAFVAELPGVCMVDALIMLFFAVL
                     YPYGKRHSNYPQLILGTIGWAIPMTMHGLNLRPLDHPIPMAAMFAFIALVTIMNDIIYA
                     RQDIEEDIKAGVGSMAVRFQHCLDALTFALVFASSAALVIAGKLGNMGAPFFTISVGGH
                     FGFFLFLAIANQRDPKSGVEWAAKRCCTSATFLLIVGMVVDLVWRS"
     gene            complement(5337..6900)
                     /gene="sptE"
     CDS             complement(join(5337..5816,5866..6236,6288..6559,
                     6608..6900))
                     /codon_start=1
                     /gene="sptE"
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /product="flavin-dependent monooxygenase SptE"
                     /protein_id="BCD52383.1"
                     /transl_table=11
                     /translation="MDQHPLKVVIVGGSIAGLTLAHCLHRANIDHVVLEKRAEIAPQEG
                     ASIGIWPNGARMLEQLGLYGDLEKLTEPLNLMHIAFPDGFSFEDLLPTTINQRFGYPII
                     FLDRQKFLETLYRKYPDKSKIVTNTRVAEVQSSEKCARVITEDGTMYEGDIVVGADGVH
                     SLVRREMWKLGDSKQPGSVPSREKTGMTVEYSCVYGISSPIVGLRAGESVNSYMDGMTV
                     LTFHGKGGRVYWFLIQKLHQKYTYPNTPRYTVEDAKRLCTDHRDTTIWKDIRIGHLWDN
                     REVVSMTALEENLFAKWHFDRIGLMGDSIHKMTPNIGQGANSAIEDAAVFSSLLVHMMT
                     SEGPGRPSSARIQRLLYDYQACRYERAEMIYHRSRFGARFQSRDDAVKLIVGRYVVPRI
                     RDRLANISSMLIANGEIIQYLPFPKRSGPGWEKFRNKGEWMPYTPFALFSAILLVQHVV
                     PLLGLTSPLLLAS"
     gene            complement(7212..8645)
                     /gene="sptG"
     CDS             complement(7212..8645)
                     /codon_start=1
                     /gene="sptG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Transferase"
                     /gene_kind="biosynthetic-additional"
                     /product="acetyltransferase SptG"
                     /protein_id="BCD52384.1"
                     /sec_met_domain="Transferase (E-value: 9e-17, bitscore:
                     50.4, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALGALPDTHILSPIDHTFPEVNTCYYLYFQRIRHGDVQLTLQRG
                     VERLVRRYPFLAGEIVDSKGSKAARYSGLRHVQESVSAANGCSFIEFRQDFSLSVKAIT
                     EATTRSGPEDLLLARKLSPLPLLPEASQRKCLVRFRATTVSDGLVLAMGFSHSFFDANG
                     AAHLLERLAECCGDDSPEPWNDPDDQSLRRTVWGLGDGIVSSRKHAEHFGPLPALAKPP
                     GGQVSLLEALTVNTMVCRWKVSAAKVEMLKRACIRLLSYQGNDARIPYLSSLDVLTSCL
                     STCLQRSKGIAGPSKAGISINLRGRLNPTWPSGYLGNLATYATVPSGSAPTQQELRAAK
                     QITYGLPRLSSLTPDLAQVYRNACSLRETISSFDDQFIRGLVSWLVSQPDLTKVNIQPY
                     VVNFTSWRHLNLYDLEFGPSLRRPCHVLTHGMPPTLCLVMPEKKVAGDGRNDWDIVSYL
                     DMDDYLRLNEDDLIRFLLE"
     gene            9270..10127
                     /gene="sptF"
     CDS             9270..10127
                     /codon_start=1
                     /gene="sptF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /product="2-oxoglutarate/Fe(II)-dependent dioxygenase SptF"
                     /protein_id="BCD52385.1"
                     /sec_met_domain="PhyH (E-value: 3.3e-35, bitscore: 111.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVPQLHYVPYDTPVEDVMRILKESGTLVIRNFLDQNTVQKVQDE
                     VDDYVRNWNPGPKYNHDIKNVGSKTKQPSNLSLMSKTYRCEVLNHPWMHAICERMFGPT
                     YGDYWFNGGSILHLEPGENTQPIHQDHVFYQISKWRRPTDPDLTINFTMALTEFTVENG
                     GTRVCPGSHLWENGHASPAEEDMVPVLMQPGDALILPGSMWHSAGANRTSEYRRGFATS
                     FHPCHFTPIESHHHLPREMVEEMTPLVQKMLGFRTLNLHNNVKVWKAGEGNLEDATGLK
                     SVAA"
     gene            11220..12092
                     /gene="sptN"
     CDS             11220..12092
                     /codon_start=1
                     /gene="sptN"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /product="2-oxoglutarate/Fe(II)-dependent dioxygenase SptN"
                     /protein_id="BCD52386.1"
                     /sec_met_domain="PhyH (E-value: 6.7e-31, bitscore: 97.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVAADATPPPLRVVDQETPREKITKVLKEDGVVRINGLLTPTVI
                     DSLEAEIDHYVRHWETGLSQDHDSYHQIVGAKTKQPSNLCVVSRTYRQHFLGHPLINAI
                     CEDFLSAEFGDYWLNGGAVLHLEPGEKAQTLHQDQYVHRVAEWRRPSDPQILITFIVAL
                     SEFTEENGATRVIPKSHLWSHSPPPASRTRPALLKPGDALVIFGSTWHGAGANYSSSYR
                     RGLACGFQPCQLTPLETHQHLPKTIVQEMTPLAQKMIGWRTMCNDKQIKMWRAGDAKLE
                     DVMGLKSAV"
     gene            order(12506..12527,12578..13428)
                     /gene="sptI"
     CDS             join(12506..12527,12578..13428)
                     /NRPS_PKS="Domain: PKS_KR (31-186). E-value: 1.3e-10.
                     Score: 33.5. Matches aSDomain:
                     nrpspksdomains_sptI_PKS_KR.1"
                     /codon_start=1
                     /gene="sptI"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /product="short-chain dehydrogenase/reductase SptI"
                     /protein_id="BCD52387.1"
                     /transl_table=11
                     /translation="MTDPASQFRNFITTQHHDTYPAIQKSCHQGHVVAITGASSGIGKA
                     TALSFARAGVSVLALIARRSLDEVESDIAMAAVAAGHQAPKVLKLVADVTDESSINAAA
                     EETRCHHGRLDVLVNNAGRVETMSSLLDGDPQDWWRTFEVNIKGVYLTTRAFLPLLLAG
                     GERTIINIGSMGAVSPIAGLSAYLSSKVALLQFSNVLTLEYGRYGLVSFTVYPGDVQTE
                     VLGSFPDAVREYFVDTPELPADTISWLTQSRLEWLAGRFISSRWDMLELLNCQDDIVQG
                     DKLKLQMTI"
     aSDomain        12649..13113
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_sptI_PKS_KR.1"
                     /evalue="1.30E-10"
                     /label="sptI_PKS_KR.1"
                     /locus_tag="sptI"
                     /protein_end="186"
                     /protein_start="31"
                     /score="33.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VVAITGASSGIGKATALSFARAGVSVLALIARRSLDEVESDIAMA
                     AVAAGHQAPKVLKLVADVTDESSINAAAEETRCHHGRLDVLVNNAGRVETMSSLLDGDP
                     QDWWRTFEVNIKGVYLTTRAFLPLLLAGGERTIINIGSMGAVSPIAGLSAY"
     CDS_motif       12658..12747
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_sptI_0001"
                     /evalue="5.70E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="sptI"
                     /protein_end="64"
                     /protein_start="34"
                     /score="9.4"
                     /tool="antismash"
                     /translation="ITGASSGIGKATALSFARAGVSVLALIARR"
     gene            order(13995..14291,14346..14711,14774..14982,15036..15489,
                     15552..15641,15694..15884,15941..16937)
                     /gene="sptK"
     CDS             join(13995..14291,14346..14711,14774..14982,15036..15489,
                     15552..15641,15694..15884,15941..16937)
                     /codon_start=1
                     /gene="sptK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /product="cytochrome P450 monooxygenase/hydrolase SptK"
                     /protein_id="BCD52388.1"
                     /sec_met_domain="p450 (E-value: 1.3e-56, bitscore: 181.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALILSGTPATLLLTSFVLYVLSKVLWAFLSLPDVPGPLWAKVTN
                     LQRLYWVRTGRAHDIHYMLHEKYGTFVRMGPNMMSISDPAALSTVYPTRMGVPKSDFYK
                     TQRPYVPGTGALPVVFNTQNEELHKELRGPVSSLYAMSNVMKLEPLMDETLQVLFDQID
                     ARFVSETKEFDLSNWLQFFAFEVMGTISFSKKYGFLEAGRDLNGLLSGIWGFMKSAAPM
                     GQMPWLDDVLYKNALAARLRGTTGMPVLSIVNKYITERITGRTKASSDHADMLSQFLDI
                     QASNEKVPTWAPKAWTFSNVIAGSDSSANSMTTVMYNLMTHPETMARLYQELSEAKQQA
                     GNVTAHILPWTSIRDLPYLDACVMEAFRIHPAFCLHLERLVPETGMEICGKQIPPGTIV
                     GMSPWVINRHKPTFGEDVHQWRPERWLGHSDTRLQELKNTILTFGYGRRVCLGKNIAIM
                     EIKKLISSLVLTYEWTVIDPSEYRVENKWFFKQSGFDVTVKHRSSVRHTPRATNMTKVP
                     PTLAIPASSSTVEVRVINTRTTMRTDHSLLWKSPVEGFKGLDLPIYAFLISNGNRHIIF
                     DLGLRQDYENLPPRIAGLLKNAPYIVTEANVSEILDSDDTGLDIKGRDIEAVIWSHHHY
                     DHTGDPSTFPPSTKLVVGPGVLSLTGGGYPKNPNTTVLETDLSGRKIQEISFDAQADSS
                     VKVGPFDGVDYFGDGSFYLLNAPGHSVGHMCGLARVTTAPDTFIFMAADGCHHPGAIRP
                     SEYIALPRDIPKSLVRKLRTAEADSGGKAQDGDTKPLLPFLPALFPDYTQAMETVEKIK
                     QLDACDNVFVILPHDGSLLGAIDFFPRPINDWKKKGLKESTRWKFCQEMEEALSG"
     gene            complement(17016..17702)
                     /gene="sptL"
     CDS             complement(join(17016..17072,17120..17264,17319..17513,
                     17566..17702))
                     /codon_start=1
                     /gene="sptL"
                     /product="hypothetical protein SptL"
                     /protein_id="BCD52389.1"
                     /transl_table=11
                     /translation="MRGLGESTETALRLGSDAKDRVYRAWDGFIDFAARDNVLEVALGL
                     IIAQAFTSVVNSFVSDIVLPLVSLLPFIMRNMDEKFAILSKGPHYQEGYNTIEQARDDG
                     ALVLAYGVFLEKIVNFLVISLTLYTLAQLYMVFSKRKIIKRTVKCKYCRKWISERALRC
                     VNCSSWQDGREDVQ"
     gene            order(19218..19796,19853..20935,20988..21363,21414..25763,
                     25812..26716)
                     /gene="sptM"
     CDS             join(19218..19796,19853..20935,20988..21363,21414..25763,
                     25812..26716)
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (379-793). E-value:
                     2.5e-150. Score: 493.0. Matches aSDomain:
                     nrpspksdomains_sptM_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (897-1181). E-value: 3.4e-60.
                     Score: 195.8. Matches aSDomain:
                     nrpspksdomains_sptM_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (1292-1382). E-value: 4.3e-12.
                     Score: 38.7. Matches aSDomain:
                     nrpspksdomains_sptM_PKS_DH.1"
                     /NRPS_PKS="Domain: PP-binding (1619-1683). E-value:
                     3.9e-12. Score: 38.6. Matches aSDomain:
                     nrpspksdomains_sptM_PP-binding.1"
                     /NRPS_PKS="Domain: cMT (1909-2069). E-value: 3.1e-56.
                     Score: 182.1. Matches aSDomain: nrpspksdomains_sptM_cMT.1"
                     /codon_start=1
                     /gene="sptM"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     itr_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     ene_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     tra_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     hyb_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /product="polyketide synthase SptM"
                     /protein_id="BCD52390.1"
                     /sec_met_domain="mod_KS (E-value: 7.7e-131, bitscore:
                     426.4, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 3.7e-77, bitscore:
                     248.9, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="hyb_KS (E-value: 2.1e-138, bitscore:
                     451.4, seeds: 33, tool: rule-based-clusters)"
                     /sec_met_domain="itr_KS (E-value: 5.5e-154, bitscore:
                     502.6, seeds: 96, tool: rule-based-clusters)"
                     /sec_met_domain="tra_KS (E-value: 2.7e-134, bitscore:
                     437.8, seeds: 44, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_KS (E-value: 4.9e-151, bitscore:
                     493.0, seeds: 2284, tool: rule-based-clusters)"
                     /sec_met_domain="ene_KS (E-value: 1.2e-47, bitscore: 152.1,
                     seeds: 19, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 6.9e-61, bitscore: 195.8,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.7e-13, bitscore:
                     38.6, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEASQGIAVLFGPQSADIEEAVSCIQAFVRENPSATYLIDIIQSL
                     PSIWPAIQDSWVPLAQIDGKRQLETLGSLFGGGAATVPPKSSNILITPLTVLQHIVELC
                     RLRKRGSSLQIKDVQGFCVGFLAATAVASAHDETQFRSIVAKVIRLAVCVGALVDLNEL
                     ERGRAASLAVRWSGEEGYSCMEKVLAAHPEAYISCLTDTNRATITVPQELQRDIIREMA
                     NQGLSVRSIPLCGRFHHPDHAPAVRQLMQLCRSDDRFQLPDSKTLQFPLRSNIDAELVP
                     EGPLHDIALQSILCLQSKWHATVSAALESLTERNGNVPIAAIGPEQCLPRTARSRLRQT
                     WGSTLVPMGTGHTVNGNLTPSRATSPADSSSELIPTVQPIAITGMSCRYPQADSVEALW
                     ELLELGKCAVGPLQNKRFRMDELLREPNGPFWGNYLEEPDVFDHRFFGISAREAATMDP
                     QQRLLLQVAYEAMESAGYCGIRSSQVPEDVGCYIGVGSDDYTDNVGSTHANAYSAPGTL
                     QAFNTGRISHHFGWSGPSVVVDTACSSAAVAIHMACQGLHTGDCSIAVAGGVNVMTSPK
                     VTQNLAAASFLSPTGASKAFDASADGYCRGEGAGLVVLRPLDQALQNGDPVLGIITGTA
                     VNQGSNCSPITVPVSRSQMSLYRKALSASGVSPDDVTYVEAHGTGTQVGDPIEMDSIRN
                     TFGGTHRTEQLYVGSIKDNVGHTETSSGVAGLIKTILMLQKQTIPKQANFSRLNPKIPA
                     LDMDNVVIPTRSTDWKVSTRVAMVTNYGAAGSNASIIVRQPFTYPGGTRTTLSHAPIFI
                     AAKTAESLRTYCDNLLASLRQIPVLPDHLVSDYAYNLATKQNRTMDCYIGFATDSPASL
                     TNQLAAVASGAREVYSRSGKALPVVLCFGGQNGNIVHISQDLYTNTHLLQFHLADCEKA
                     CQSLGLPSLFPTIFRADPIEDLVSLHSALFATQYATAKCWIDSGLEVDRMIGHSFGQLT
                     ALCVAGGLSLVDALRFISERAHLLEKHCSGKRGLMLSVEASEEDVRGLVNGAGQAVDVT
                     CYNGPRSFVIGGEKVAIEAFEKVATGFKTQRLANTHAFHSHLMDPILPALREVAQSMTF
                     QPTTIPVEACSQDMEWTFVHAAQLVTHTRQPVYFESAVRRIANRYPTGAVWLEAGSASP
                     VIPMIRRVVESSPNNHVYQPIDIRPPQAIANLSKATSGLWSNGTHVQFWPFHKSQSNCY
                     NSINLPPYQFTKTRHWIDYDPLAFMPSSAPKDAGPEEAKKLVQLLKQQSGECLFTINTK
                     DLLYQTCTKGHAVVNQNLCPASLYLEMVISAASHVCPVELSSTMPHVQNLCILAPLVLN
                     PQGELQLRLSQQNGEKDQWTFSLFTQQGQTSTVHATGTIWLHAITNSSAIVSRFRSLNR
                     LMIPSRPDSIENSPRSSGLKGAAIYQSFRRVVNYAEYYRGVENVFALTNEATGQVKIDT
                     ALVKHSCCDPILIDNFIQVAGIHVNCLSEMSEDEVYVCSAVGEIFIGEAFMQRDSGAAL
                     SWRVYSNYDRLSRNQVACDVFVMNQESGQLAIAIMGATFTGVSIRALTRTLARLNNQQS
                     SAPEVHDAPIPQDTVRADAMPAATPAPLPSTLQEVDNLPAVQGMLGDLLGVGLEELAPS
                     CSLLEIGVDSLMSTEVLTEIKKRFNVNITSAALSEIPDIGGLVQVIFPGTSVSQTTVSQ
                     TAPNDSNAVVVESTPVGELSMLVQEAYDAFDAIRSMTDYSRETKWTGFYDIVFPKQMAL
                     VTAYVVEAFQALGHPLQSISAGGEVPLIPVLPQHEKVRNQFYAVLEFSRLVSRTTDGRI
                     VRTKEQIPTEAASNLHDEIIRQFPHHASEHMLLRTTGSQLAECLSGDANPLALLFQDAE
                     ARRLMGDVYTNAPMFKSATMHLAGYLQGVLERVGSGRTVKILEIGAGTGGTTRYLVSQL
                     AARGLRFEYTFTDISSSLVMLAKKGFKEYDFMRYMTLNIEQDPPQELLGQYDIIISTNC
                     IHATRNIAYSCSNIRRMLRPDGILCLIELTRNLFWFDLVFGLLEGWWLFDDGRKHALAS
                     EHLWNETLDKAGYSWIDWSRNELKESEFLRLIVASPSKPEMPLVTQETVKFDEKDGVSL
                     LADIYYPSVADDANQRRPIALLLHGGGHIMLSRKDIRADQTMLLLDAGFLPVSIDYRLC
                     PETSLIEGPMRDARDALRWARQTLPTLPLQREDIHPNGDQVVAIGWSTGGHLAMSLAWT
                     AAELAVAAPEAVLSFYCPTDYTDPFWSQPNFPFGKQIAPPGEIDIRAGMSPTPITAYNP
                     PRSKRALGGWMSTTDPRSQIALHMNWKGQTLPVLLNAAQTAPGEKLLDPTKEDIVRVCP
                     TSQASAGNYRSPTFIIHGSLDDLIPMSQVRHTAKVMKANGVDVQLRELDGSIHLFDIAS
                     TYGDKPDEVQAVADGIQFLKDRVQC"
     aSDomain        join(20411..20935,20988..21363,21414..21754)
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_sptM_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="2.50E-150"
                     /label="sptM_PKS_KS.1"
                     /locus_tag="sptM"
                     /protein_end="793"
                     /protein_start="379"
                     /score="493.0"
                     /tool="antismash"
                     /translation="IAITGMSCRYPQADSVEALWELLELGKCAVGPLQNKRFRMDELLR
                     EPNGPFWGNYLEEPDVFDHRFFGISAREAATMDPQQRLLLQVAYEAMESAGYCGIRSSQ
                     VPEDVGCYIGVGSDDYTDNVGSTHANAYSAPGTLQAFNTGRISHHFGWSGPSVVVDTAC
                     SSAAVAIHMACQGLHTGDCSIAVAGGVNVMTSPKVTQNLAAASFLSPTGASKAFDASAD
                     GYCRGEGAGLVVLRPLDQALQNGDPVLGIITGTAVNQGSNCSPITVPVSRSQMSLYRKA
                     LSASGVSPDDVTYVEAHGTGTQVGDPIEMDSIRNTFGGTHRTEQLYVGSIKDNVGHTET
                     SSGVAGLIKTILMLQKQTIPKQANFSRLNPKIPALDMDNVVIPTRSTDWKVSTRVAMVT
                     NYGAAGSNASIIVRQ"
     aSModule        20411..24424
                     /complete
                     /domains="nrpspksdomains_sptM_PKS_KS.1"
                     /domains="nrpspksdomains_sptM_PKS_AT.1"
                     /domains="nrpspksdomains_sptM_PKS_DH.1"
                     /domains="nrpspksdomains_sptM_PP-binding.1"
                     /iterative
                     /locus_tags="sptM"
                     /monomer_pairings="mal -> mal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       20870..20917
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_sptM_0001"
                     /evalue="3.40E-05"
                     /label="PKSI-KS_m3"
                     /locus_tag="sptM"
                     /protein_end="548"
                     /protein_start="532"
                     /score="16.1"
                     /tool="antismash"
                     /translation="GPSVVVDTACSSAAVA"
     CDS_motif       21111..21149
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_sptM_0002"
                     /evalue="4.50E-05"
                     /label="PKSI-KS_m4"
                     /locus_tag="sptM"
                     /protein_end="608"
                     /protein_start="595"
                     /score="16.3"
                     /tool="antismash"
                     /translation="FDASADGYCRGEG"
     CDS_motif       join(21342..21363,21414..21439)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_sptM_0003"
                     /evalue="6.20E-05"
                     /label="PKSI-KS_m5"
                     /locus_tag="sptM"
                     /protein_end="688"
                     /protein_start="672"
                     /score="14.8"
                     /tool="antismash"
                     /translation="YVEAHGTGTQVGDPIE"
     aSDomain        22067..22918
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_sptM_PKS_AT.1"
                     /evalue="3.40E-60"
                     /label="sptM_PKS_AT.1"
                     /locus_tag="sptM"
                     /protein_end="1181"
                     /protein_start="897"
                     /score="195.8"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="CFGGQNGNIVHISQDLYTNTHLLQFHLADCEKACQSLGLPSLFPT
                     IFRADPIEDLVSLHSALFATQYATAKCWIDSGLEVDRMIGHSFGQLTALCVAGGLSLVD
                     ALRFISERAHLLEKHCSGKRGLMLSVEASEEDVRGLVNGAGQAVDVTCYNGPRSFVIGG
                     EKVAIEAFEKVATGFKTQRLANTHAFHSHLMDPILPALREVAQSMTFQPTTIPVEACSQ
                     DMEWTFVHAAQLVTHTRQPVYFESAVRRIANRYPTGAVWLEAGSASPVIPMIRRVVESS
                     PNN"
     CDS_motif       22307..22414
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_sptM_0004"
                     /evalue="8.20E-13"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="sptM"
                     /protein_end="1013"
                     /protein_start="977"
                     /score="40.4"
                     /tool="antismash"
                     /translation="DRMIGHSFGQLTALCVAGGLSLVDALRFISERAHLL"
     aSDomain        23252..23521
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_sptM_PKS_DH.1"
                     /evalue="4.30E-12"
                     /label="sptM_PKS_DH.1"
                     /locus_tag="sptM"
                     /protein_end="1382"
                     /protein_start="1292"
                     /score="38.7"
                     /tool="antismash"
                     /translation="KGHAVVNQNLCPASLYLEMVISAASHVCPVELSSTMPHVQNLCIL
                     APLVLNPQGELQLRLSQQNGEKDQWTFSLFTQQGQTSTVHATGTI"
     aSDomain        24233..24424
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_sptM_PP-binding.1"
                     /evalue="3.90E-12"
                     /label="sptM_PP-binding.1"
                     /locus_tag="sptM"
                     /protein_end="1683"
                     /protein_start="1619"
                     /score="38.6"
                     /tool="antismash"
                     /translation="VQGMLGDLLGVGLEELAPSCSLLEIGVDSLMSTEVLTEIKKRFNV
                     NITSAALSEIPDIGGLVQV"
     aSDomain        25103..25582
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_sptM_cMT.1"
                     /domain_subtypes="cMT"
                     /evalue="3.10E-56"
                     /label="sptM_cMT.1"
                     /locus_tag="sptM"
                     /protein_end="2069"
                     /protein_start="1909"
                     /score="182.1"
                     /tool="antismash"
                     /translation="GRTVKILEIGAGTGGTTRYLVSQLAARGLRFEYTFTDISSSLVML
                     AKKGFKEYDFMRYMTLNIEQDPPQELLGQYDIIISTNCIHATRNIAYSCSNIRRMLRPD
                     GILCLIELTRNLFWFDLVFGLLEGWWLFDDGRKHALASEHLWNETLDKAGYSWIDW"
ORIGIN
        1 gtcagattcc ggctgtataa ctatccgaat gtcatgctat cacgacttgt ccccgcagac
       61 ggtggaataa tgatccccgt cgaaggggga caagcactcc ttaagcagtc ttagacttca
      121 gacccaaagc ttcgtgaaag tccttatcct gcaacatatt cagaccgtag gtgtccttgg
      181 tccggacagt accccaaccg atcatcctct gcgccagggg cgtcattgtc tcaacaatgt
      241 ccctcggaac attaacgttg gactcgcgcg gcgtaaggta accaggctga gcagcaagcg
      301 cgagcccaat acgcggctta tcagagcggt tggcaccacc tccatgaacg cacttgccag
      361 tgaagatcag ggcatcgccg acctgcatct cagcagcggg gagatcctcg gcgttggcgt
      421 agaaatcggc attcggcccc gtgtgactct taggaagcaa gagagtaccg ccattatcgt
      481 gccggaactc gttcagcggg aaggtaatgt tgatcatcag atcggggctc gtcgcagggt
      541 ccatgtgctg agagacaggg tagaggtagt catcacggtg tggtctctgc gcctcagcac
      601 caggcaacat gttcaggatg ttacccatgt tcagccagta gtcgccggaa tccttcttga
      661 acaccttttc gagaacctca tgggcaaagg gcaagttcag cagctcgtcg cggtaagact
      721 tggacgtgag gacgaggtct cccatgtgga tggtgcggtt gtgcatgttg atgatgtgtg
      781 tctgttcgcc cttcttggcg ccgatggtag ccttgtccat gacgcggttg acatcgtcac
      841 tgattttcgc gatgatgttg gggcaaatga cttgctcgac tatgacgcaa ccgtcttctt
      901 gaaggatctg ataggcggcg tcgctgccct gagaggcgtt gacgcggcgg ataggagggt
      961 agttcttaga ttcgatggtc attttagtgg tggctagctg gtaagaacaa tgagttcgtg
     1021 aatgtgtggt atgtcaagga gctttctaga caatttcagg tcttcaatgc ccttttcaaa
     1081 gaatatagct ctaccaggac tgtctgaact tcggtgtgac ggccatcccg gcctccttgc
     1141 atctctacta gcaactagga tgcagttttc cggtgtagct ctctgcataa gtgcagggtg
     1201 tcttatccgg ccacccagat ttgccgcagc ggactgttgt aagtgttcag tatgcatacc
     1261 taccacacct gtggaatgga cataatgaag ctgctctgtc acaccttgac gtcgagttga
     1321 gtgggacaga aggatgccat tcgtgactgg ggcatgcttt tcctgggatc gtcgccggaa
     1381 tgcgaaaatt acctgaagaa taacttcaat gagttattcc agatctctca ccctcacgtc
     1441 tttatcgtag gtggcttgca tgcttacact tcctcgctga tgcaaggagc gtaacgcgag
     1501 ggagaaaggg acagagaaac agtcagggct ctttccgcca aacgaacctg tcgagccact
     1561 tactgccagt atgcataaac ccccaacaag gaattgtgta agtagatatt acttgtgcat
     1621 tgctttatcc caacaaactg cattggaacg acgttattta aggggtgtgg cacggtgtag
     1681 cctcatcctc ccgacacaca ggtctaccac ggactagcca tgcagcccat cactcagatt
     1741 cctctcaatt ttgatacggt cgtgaacctt ctcggttcag ccagcggcat aggatggatc
     1801 ctcaattaca ctctgatgac ctactactcc ttccgcgata agacatacag catgtcgatg
     1861 ctgcctctat gctgtaacat tgcatgggaa ttcgtctacg gcgtcctctg cccctcgtcg
     1921 acgtttgtcg tgcgccccgt catcctctcc tggcttgttc taaactgcct tgtcgtatac
     1981 gcggcaatca agtactcgcc gaacgaatgg gcccacgcac cacttgtcca gcgtcattta
     2041 ccgcttcttt tcaccgtcgg aatagccgcc tgcacgggct ttcacatagc gcttatccgg
     2101 aaattcgacc cagccactgc attcttatgg agcgctaggt catgccaggt gctgttgagc
     2161 atcggcgggt tattccagct tctctgccga agtagcacaa agggagggtc gtatgttcta
     2221 tggtatgcct atgcaatgat tgtcccctcg catcagacgt caaaggcagt cctaataaat
     2281 ggatttctgg caggctatct cgcttcctcg ggagcatttg cggtgtattg aagatgacgc
     2341 tgatgtggaa gtacggagag tctcggttcc cgtggctcga tgatccactg acggcgtact
     2401 gcattgcatt gtggattatc tctgatgtac tttacggtgt ggtgttctat agccttcggt
     2461 ctaaggagct ggcaggcgca ggcaaagcga aggccattta ggcggagagg gtctggactg
     2521 gtttatctgg cacatagctc agtgcatttc agttagccac tacatatcat gacgaataaa
     2581 tagtaacacg gatcaaaaat tgccttagct gcaggagctt cccgcatatc cccctttcgg
     2641 cgagaatggg gcccgcaaag catataccaa ctggaaaaga cttgcaaggg ataatggtac
     2701 taggggcagt cgaccactcg ggctgtaggc ttagaccgct ggttgtatac atgcgtcaat
     2761 ggctgaagat gaggtacaca gctgatggac agtcaaggtc gggcagcggt acggcacgca
     2821 gattcccctt ggcgcattgc agtgcatgca gcagcagaag gaagctgtgg gcctcgagaa
     2881 caagacgtct gggaaggctg aacatacggt gagcggatgg gaggaaccca gtgcccctgc
     2941 tagcaacgat aatgatagtg gttgtttgtc tcactccgta gttggtacag cgtcaattac
     3001 tgactagtca gcatgggatt ccttcaagac aaggtagtca agagactctg cgatgccggt
     3061 aacctatatt ttgctaatca aaggtcatct aggttgtgat aattactggc gcggcggcag
     3121 gaatagggct tgcaaccgca accgcagcac tagatgaagg cgcccgcgta ttcggcatcg
     3181 acctcgcacc agccccaaca agcctgcaag aaaatccgaa cttcgaattc ctacgtgccg
     3241 atctgacaga ccatgactgt ccaaagcgag ccgtgcaagc ctgtatcgcg gccttcggac
     3301 agcgtattga cgggctactg aatgtggctg gtatcgcgga caactacgga agcgctgaca
     3361 gtgtgactga tgaagtctgg gatcgttgtc tggcggttaa tttgactgcc ccggtgaaat
     3421 taatgcggga ggtgatcccg ataatgagag aggcgggaaa aggtagtatt gtgaatacgt
     3481 cgagcaaggc aggactgagt ggagcgtcct caggcgtggc gtacacggcc agtgagtctc
     3541 ttcccagtag aatccatttg caaagcgcaa ggctaaaaag gtaaaggcaa acatggcctg
     3601 atcggagtga ccaagaatgt cgcctggcgg ttcaaaacgg agggcatccg gtgcaacgcg
     3661 atctgtcctg gaggtatgtc acattctagt cggttttttt cctggagaat attcgctgaa
     3721 agctgccata gcggtggcaa cagaaatggg aatgacagga gacttctcgc tctgggacca
     3781 agctgctatg gaagcgatgc gtcctatcca cgcagcacat atggatatgg gaataggagc
     3841 gactatcaaa gcgcaggagg ttgctcaaac gctgttgttc ttggtttctg acttgagcag
     3901 aagagtaaat ggggcagtaa ttccagtgga tgacgcttgg tcaacgatat agatactgca
     3961 atatgtagaa ggccaggtta ctcaagagga attgagacag ctgaatggag attctataga
     4021 ggatgagtta cttcagaata tttttcttga tttccgacaa catagtacgc tattttgctg
     4081 aacagaatga atgatacagt atgatgggag gaacgcaaaa tgcaccccag atattgtaat
     4141 caccagagga tgaatcagga ccgccagacc aaatcgacaa ccataccaac aatcagaaga
     4201 aaagtcgctg aagtgcagca tcgcttagcc gcccactcaa caccagactt ggggtctcgc
     4261 tggttcgcta tggcaaggaa taagaagaac ccaaagtggc caccgacgct gattgtgaag
     4321 aacggagcac ccatattccc cagcttgcct gcaataacga gagcagcaga tgaagcaaac
     4381 acaagagcaa atgtcagagc atctaggcaa tgttgaaagc gcactgccat actcccaacc
     4441 ccggccttga tatcttcctc aatatcctga cgcgcgtaga tgatgtcgtt catgatggtc
     4501 acaagtgcaa taaacgcgaa catcgcggcc ataggtatcg gatggtccag cggacgcaga
     4561 ttaagaccgt gcattgtcat gggtattgcc cagccgatgg tgcctaagat aagctggggg
     4621 tagttgctgt gccttttgcc atagggatat aggacggcaa aaaatagcat gataagagca
     4681 tcgaccatac agaccccagg cagctcagca acaaatgcaa agccgcagag gaatgtccca
     4741 gcagcgaata tcgtggcctg cgagtttgat accgcgccac gaggaagagg cctcaatcta
     4801 gtgcgagcca ccttcgcatc aacatgctgg tctgcgatat catcccatat acatccaccg
     4861 ctccgaagga aaattgacca taaacataga agcgtgatcc gaggccaaaa gtcggcttcg
     4921 aggacttcgg gtgcgatcga ggcgctgaat gcccctgcaa cgagaaacgg gtagcagacg
     4981 aggatgtatc ctaggggctt atgtagacga aggagctccc cgtaaggtag gaacgaggtc
     5041 ggcaaccgag aaagaacctt ggacggagga tggtatcttc ttggaagccg ctctttgcag
     5101 agaaacgttt gtttggcttc ggtagttgtt gcggacatgg ttgctagcgt acaagatgta
     5161 caacggtgta tctgcgaaag tgcaaaaaca catatactaa ggacaatgaa attctcaatg
     5221 actgtttaat agagttaatc tatagctaga caatggaccc gagactgagc tcgccgactt
     5281 ccagcatgga accactctgc acccccttgc agcctggaat gcaactatga ccatatctat
     5341 gaagcaagga gaagtggcga ggtcagaccg agcagtggaa caacatgctg tacaagcaat
     5401 attgcagaaa acagagcgaa aggggtatac ggcatccact cccccttgtt gcggaacttc
     5461 tcccagcctg gtccggatcg cttcggaaag ggaagatact ggataatctc accattggca
     5521 atcagcattg aggaaatgtt agcaaggcga tcacggatgc gggggacaac ataacggccc
     5581 acaatgagct ttacagcgtc atcacgcgac tggaaccgag ctccaaagcg ggacctgtga
     5641 tagatcatct ctgccctttc gtaccggcag gcctggtagt catagaggag acgttggatg
     5701 cgcgctgatg atggtcgtcc ggggccttcg cttgtcatca tatggaccag cagcgaggag
     5761 aataccgcgg cgtcttcgat ggcactgttg gctccctggc caatgttggg tgtcatctgt
     5821 ctcgctttag cctggcccta tagacctatt atagatggta ctgaccttgt gaatactatc
     5881 tcccatcagg ccgatacgat caaagtgcca cttcgcaaac aggttttcct ccagggccgt
     5941 catcgagacc acctcccgat tgtcccacaa gtgtccgatc cgtatgtctt tccagatggt
     6001 cgtgtcgcgg tggtcggtgc agagcctctt ggcgtcttcg actgtatagc gcggcgtatt
     6061 gggataagtg tatttctggt ggagcttctg gatgaggaac cagtagacac gccctccctt
     6121 tccatgaaaa gtaagaacgg tcattccatc catgtaagag ttcacactct ctcccgcgcg
     6181 caggccaaca atgggcgacg atattccgta gacacaggag tattccaccg tcatgcctat
     6241 ggatagtcag atagtgaaat tggacttttg gggcactatg gactaacccg tcttttcacg
     6301 gcttggaact gaaccgggtt gtttagagtc tcccagtttc cacatttccc ttctgaccag
     6361 actgtgtacc ccgtctgctc ctaccactat atcaccctcg tacattgtcc catcctctgt
     6421 gattacccgg gcacacttct cagacgattg gacttctgcg actcgtgtat tggttactat
     6481 cttggactta tccgggtact ttcgatacag ggtctcgagg aacttctgac ggtcaaggaa
     6541 tataatcgga tatccgaaac taagaaaatc agcatcgtcc ataaggtggg atagttagac
     6601 agcccaccgc tgattgatag ttgttgggag cagatcttca aaagagaagc catcagggaa
     6661 agcaatgtgc atcaaattca aaggctcagt caacttctcc aaatccccat agagcccaag
     6721 ctgctccagc atccgggcgc catttggcca gatgccgatg gaagctccct cttgaggtgc
     6781 aatttccgca cgcttctcaa gaacgacgtg atcgatattt gcgcggtgca ggcagtgtgc
     6841 cagggtaagg ccggcaattg atccccctac gatcaccact tttagtgggt gctggtccat
     6901 ggtaagagac ttcggccagg gtagtggtgt aggtagtgtg agtgacaatt cgagtgggag
     6961 acagccgacg cagctcagtt taccatttct taggccaggg cggaatgacc gggggccatg
     7021 cgtcgcacta cgtgtcgcaa ggggtgatca atcggggtct acctgcaaac gagacaaaat
     7081 gaaatgaatg aagcaagaat tctttattaa agggcaagaa aaagagacat tacactaacc
     7141 cagaacaacc ataagctatg acgcgagcat ctacatggac agaataagag atatcatgta
     7201 agcttatagc ctcactctag caagaaccga attagatcat cctcattcaa ccgcaagtaa
     7261 tcgtccatgt ccaagtagga cactatgtcc caatcatttc gtccgtcgcc tgcaaccttt
     7321 ttctcaggca tcacgaggca gagtgtgggt ggcatgccgt gagtcaaaac atgacatggg
     7381 cgacgcaatg agggaccaaa ctcaagatcg tagagattga gatgcctcca gctcgtaaag
     7441 ttgacgacat aaggctggat attgactttg gtaaggtcag gctgagaaac gagccaagag
     7501 acaagacccc ttatgaattg atcatcgaat gacgagattg tctcgcgcaa tgaacaggca
     7561 ttgcggtata cctgtgccaa gtccggagtc agactagaca gacgaggaag accatacgtg
     7621 atttgcttag ccgctctcag ctcctgttgt gttggcgcgc tgccgctggg gactgtggca
     7681 taggttgcga gattccctaa atacccactg ggccacgtag ggtttagacg cccgcgaaga
     7741 ttgatggaga tgccagcttt actaggccct gcgattccct tactgcgctg caaacatgtg
     7801 gacaagcacg aggtcaggac gtcgaggctg gacagatacg gtattctggc gtcgttcccc
     7861 tgataggata acagcctgat gcatgccctt ttcagcattt caaccttggc cgcagagacc
     7921 ttccaccgac aaaccattgt gttgacggtg agagcctcca atagagaaac ctggccaccc
     7981 ggaggtttgg cgagggcagg aaggggtcca aagtgctctg catgtttcct cgagcttacg
     8041 atgccgtccc ccaagcccca gactgtccgt cttaggctct ggtcatctgg atcgttccat
     8101 ggctccggac tgtcatctcc acaacattca gccagacgtt ccaacaggtg tgccgcaccg
     8161 ttggcgtcaa agaaggagtg tgagaacccc atggcgagaa cgagaccatc gctgacagtg
     8221 gtagccctga agcgtactag acattttcgc tgagaagcct cgggcaacag aggtaaagga
     8281 gacagttttc tggcgagcag caaatcttct gggcccgatc gtgtggtggc ttcagtgatc
     8341 gccttgacgg agaggctgaa gtcctgacga aactcgatga atgagcaacc gtttgccgca
     8401 ctgactgact cctgtacatg ccgaagtccg ctgtatcgcg ctgcctttga acccttcgag
     8461 tccacaatct cacctgcaag aaatggataa cgacgcacta ggcgctcaac accccgttgt
     8521 agtgtgagct gcacatcgcc atgacgaata cgttgaaagt aaagatagta gcacgtattg
     8581 acctccggaa aagtgtggtc tatgggggac agaatatggg tatccggtaa cgcgcctaac
     8641 gccattttgg gcaacccaag attgtaggta agaatatggt gacattcaac atggatgtca
     8701 atcagagcgc tattcacggg gcatgctgat tagcccatag gcttctgttg gcattttcgc
     8761 tgcatcaaaa agccacaagg aatgccgcac aaccattata tgtgtatgta aggataggag
     8821 caaggggcag ttcctcgcgt tacagggagc atgcctcacg cagtgagggc tttggtttgc
     8881 tgtattgatc tactaacatt tagaagcagg gtatgcacat caagcgggaa gggagttctt
     8941 gtggaatatt tccaggcaac gcggcacggc cccggggagg tcgacgatag accgcttgtt
     9001 caagacgtta gccagcaaaa actgcatcca ttgcagtgac cgacagggtg attgcaaagc
     9061 gcagccaagt gcccacatcg ccgatcttta gaatgtaggc agtaagttga tcccatggct
     9121 atttaagccg tcttccgttg tctggtgtgg attgcaaaca atggcctaga cataaatatc
     9181 cacccctgta ttgtcaaaat aacagttcat gacctttatc cctggagact accggaacaa
     9241 ccaagagagc cttcttcttc atcggcgaaa tgaccgttcc ccaacttcat tatgtgcctt
     9301 atgacacccc tgtcgaggac gtgatgcgta tcctgaaaga gagtggaact cttgttattc
     9361 ggaacttcct cgatcaaaac acggttcaaa aggttcagga tgaggttgac gactacgttc
     9421 gcaactggaa tcccggccca aaatacaacc acgacatcaa aaatgtgggc tcgaaaacca
     9481 agcagccatc caacctcagc ttaatgtcca agacatatcg atgcgaagtc cttaaccacc
     9541 cctggatgca cgccatctgc gaaaggatgt tcgggccaac atatggtgac tactggttca
     9601 acggtggctc tatcctccat cttgagccag gagagaatac gcagccaatc caccaggacc
     9661 atgtgttcta tcaaatcagc aagtggcggc gcccaactga tcccgacctt accatcaact
     9721 tcacgatggc acttaccgag tttaccgtcg agaatggagg aactcgtgtc tgtcctggaa
     9781 gccatctctg ggagaacggc cacgcctcac cagccgagga ggacatggtt cctgtcctca
     9841 tgcagccggg cgatgctctc attctccctg gaagcatgtg gcactccgct ggcgccaacc
     9901 gcacctctga gtaccggcgt ggatttgcta cctctttcca cccgtgtcac tttaccccaa
     9961 ttgagagtca ccaccactta ccgcgggaaa tggtggaaga aatgaccccc ctggtgcaga
    10021 agatgctggg tttccggaca ctcaacctgc acaataacgt caaggtctgg aaagctggag
    10081 aaggcaacct cgaggatgca accgggctga agtccgttgc tgcgtaaatg aggccacggg
    10141 ttcagggcat tggcgtttct ttcgcagaca taacagtgac cctacgcgag gatacagatt
    10201 gctaacaatg tacctacact tctttgcttg gtaagagaaa ttggggatta tggttattgg
    10261 gatcttgcta gttttactaa gtttagactg ctactcaagt acccaagagc ctgctggtca
    10321 gtgatatctg aataaaaggc acgagaagtc atgctttttc ctataagccc tatcatgctc
    10381 ttaccaaaca catcccttgg ccatgtcatt gtaactctga tagagtagtc aagaataatg
    10441 ataagtgatt caacgacaag cgcatcttat caaaatcgtt cgctgcgaat tccttaggtc
    10501 gataatggcc tggaaactta acattgggtt ttactttggg agtagacaag gaagatacaa
    10561 ccctgggtgg ctagaggcac acacaaagtt atttcccttg tccttgctat ctataaaggc
    10621 tgttgagtct gctgaggtgt aggtgctcgt agcttcaggt aaaatgatac ataattgcat
    10681 caagcccgag cgagcgaccc taggtcagaa aattagaaag aaaatttgcc ttacattcca
    10741 tggtttgcag gtaaggtgca acgccccccg tgagtccagc gcaaatgcaa aagttgatgc
    10801 ctagggattc acttgaccct aggccctcat acccttgaag tatcggtaca ggcaaacatc
    10861 atggcttgta cttggcgtcg aagcaaggga agtgtgccta tcgaagggag gtatgtttct
    10921 atacgcgatg tggtaatgga ccatgcatgc cgtttgttct gacacgtaac ctaccttgca
    10981 ggatccactg gggtctttga gcagtagtcc ccggcattta cgtaaggttt ccaatgccac
    11041 ctacaccgct ggtgctcagc gccatattga gtgtacagga gtgactgacg cactggtagg
    11101 gatatgctgg cgaggttaaa gaataatatg agccctgaaa gttgagagtt agtacatctt
    11161 ctttgactcc taacattgtt atattcctat ttcgatctcc agatacacta ccgttcacca
    11221 tgaccgttgc agcagacgcc acgccaccac cgctgcgtgt agtcgaccaa gaaaccccga
    11281 gagagaagat caccaaagtc ttgaaggaag atggcgttgt tcgtatcaat ggtcttctga
    11341 ctccgacggt cattgacagc cttgaagctg aaattgatca ctacgtccgt cattgggaga
    11401 caggtctaag tcaagaccac gacagctacc accagatagt cggtgcaaaa acaaaacaac
    11461 cttccaacct ctgcgtggta tccagaacat accgccagca ttttcttgga caccctttaa
    11521 tcaatgccat atgcgaggac tttctctcag ccgaatttgg cgactactgg ttaaacggtg
    11581 gcgcggtcct acatcttgaa ccaggggaga aggcccaaac actccatcaa gaccaatatg
    11641 tccatcgagt cgcagaatgg agacgcccaa gtgaccctca aattctgatt accttcatcg
    11701 ttgccttgag cgagttcacg gaagaaaatg gcgctacacg cgtgatcccc aagagtcacc
    11761 tctggtccca ttctccgccg cccgcaagtc gcacacgccc tgcccttctg aagcctggtg
    11821 atgccctggt aatcttcggt agcacatggc atggggcagg tgcgaactac tcctcgtcat
    11881 accgccgggg tcttgcttgt ggctttcagc cctgccagtt gactcctttg gaaacccatc
    11941 agcatttacc gaaaactatt gttcaggaga tgacgccatt ggcgcagaag atgatcggat
    12001 ggcgcacaat gtgtaacgac aagcagatca aaatgtggcg ggctggcgat gcaaagctgg
    12061 aggatgtcat gggtttgaag tctgcagtgt agtaggaata gatactccgt tggcagctgt
    12121 tgcgattcga aattgggcta gaattcacac ataggacccg ggtattgatg agttcgatca
    12181 tggactacta gggatatcgg actaaatgac gtgccgtatg gtataggcaa cgtccttgca
    12241 atgttgctta ggctttcttt atatgctaga ggagatttag actagaatat gacactattt
    12301 gaccacgcat tttgaccgta ccaccaccgt aactgctcac tggttgttca tagaccctgg
    12361 ctctcaccga tggatgcccc ttgcctttat atggacttac ctcgacccac aagaaaggcg
    12421 ctgtatattt aagcacacac aattgttcca gcagacacca acatagagtc caggaaaact
    12481 tcaaagacgc tccccaagct tcgacatgac tgatcctgca tcccaatgtc cgtgaatccc
    12541 tcttgaccaa atatgatacg gttgctaagc tccatagttc gcaattttat cacgacgcag
    12601 caccatgaca catacccagc aatccagaag tcttgtcacc aaggccatgt ggttgccata
    12661 acaggagcat ccagtgggat agggaaggca acggccctgt cgtttgctcg agctggcgtc
    12721 tccgtacttg ccttgatagc tcgccgctcg ctagacgaag tcgaatctga catcgctatg
    12781 gcagcggtcg cagcgggaca tcaagctccc aaagtactaa aattggtggc tgatgttacg
    12841 gacgaaagta gcattaacgc agccgccgag gagacaaggt gtcatcatgg ccggcttgat
    12901 gtcctggtga ataatgctgg tcgtgtcgaa accatgtctt ccctgcttga tggtgatccg
    12961 caggactggt ggcggacatt tgaagtcaac ataaaaggcg tctacctcac tacgagagct
    13021 ttcctacccc tgcttctcgc cggcggcgaa aggactatta tcaatatcgg ttccatgggg
    13081 gcagtttcgc caattgctgg tctctccgct tatctgtcca gcaaggtcgc ccttctccag
    13141 ttcagcaatg ttcttacatt ggaatatggg agatatggac tcgtgtcttt tactgtgtac
    13201 cccggggatg ttcaaacaga ggtccttgga agcttcccgg acgctgtacg cgaatacttt
    13261 gttgataccc ccgagttgcc agcagataca atctcatggt tgacgcaaag ccggcttgag
    13321 tggctcgcag gacgttttat aagttcacga tgggatatgc tagagctgtt gaattgccag
    13381 gatgacattg tccaggggga taagctcaag ctacagatga ctatatagtc tgggagttgt
    13441 tatctcgagc agtgtcctcg ctacaaaacc atgaataaca cgacactgct gtccttggac
    13501 tccatagctt agtatatgtc agcatcgtct cctctaagta cataactgcg gccctgggcc
    13561 caggtaaaca cctttctggt gtatctggtg agcattgcgt tgctcggtca cagttttgtc
    13621 catcttaaat ctggagcccc tactagtatt tgtcggtgta gattcctgac gacccattcg
    13681 aggcccttgc gtcacaacta tccactctca tgcattccgc atagcaatat ccaagacaag
    13741 catagaacta ctcacatgcc caattttcac ggctaggtgc catgagtgag gagcggcatg
    13801 caattgaaac cccctcctcc cgtgcgtcaa tctggcttga ttgtgatcag ggcatcatgc
    13861 aagggttgta gacgtcccat cagggtcacc cgcatgaatg ggaagtcgac gataccttgt
    13921 catctcatag cctaagagca gtattctgag catagattct cctcaacctg tgccattgac
    13981 agccgcaaga caagatggca ctgattctca gcggcacacc cgccaccctg cttcttacct
    14041 catttgtatt gtatgtcctg tccaaggtac tatgggcttt cttaagccta cccgacgtcc
    14101 ccggtccact gtgggcaaag gtgaccaacc ttcagcggtt gtactgggtg aggaccggca
    14161 gagcccatga catccattat atgctgcatg agaagtacgg cacatttgtc cgcatggggc
    14221 ccaatatgat gtcgatttct gaccccgcgg ctttatccac ggtttatcca acgcgcatgg
    14281 gggttccaaa ggtagtaggg ttcttatatg catggagcca atttgaagca tgctaagccc
    14341 attagagtga tttctacaag acacagagac cttacgtccc gggaaccggt gccctgccag
    14401 tcgtcttcaa tactcagaat gaggagttgc acaaggaact gcgagggccc gtctcatcac
    14461 tgtatgcgat gagcaatgtg atgaaattgg agccattgat ggacgagaca ctgcaggtgc
    14521 tttttgatca aatcgatgct cggtttgtga gtgaaacaaa ggaatttgat ctttcaaatt
    14581 ggctgcaatt cttcgccttc gaggttatgg gtacaatctc gttttcgaaa aaatatggct
    14641 ttctggaagc tggtcgagat ttgaacggcc tgctgagtgg gatctggggt tttatgaagt
    14701 cggctgctcc tgtacgtaga ttaccagggc ttcgtactta tatactcgtg ttcctctgat
    14761 tgacgtgcta cagatgggtc agatgccctg gttagacgat gtgctctaca agaacgcttt
    14821 ggcagccagg ctcaggggca ccacgggcat gcctgtcctg agcattgtca acaaatatat
    14881 cacggaacga ataacaggac gcacgaaagc ctcatctgat cacgcagaca tgttgtctca
    14941 gtttctggat attcaggctt ccaacgaaaa ggtcccaacg tggtaagctt tatgtctttg
    15001 tgataagtct tcgcaatctt tctgattctg aatagggcgc ccaaggcatg gacattttcg
    15061 aacgtgatag cgggctcaga ctcgtccgcg aacagcatga ccactgtgat gtacaacctc
    15121 atgacgcatc ctgagacaat ggcacgcctt tatcaggagt tatcggaagc aaagcaacaa
    15181 gccggcaatg tgactgctca tattctccct tggacatcga tccgcgatct tccgtacctt
    15241 gacgcgtgtg taatggaagc gttcagaata catccagcct tctgccttca tttggaacgg
    15301 ctggtcccag agactggaat ggagatatgc ggaaaacaaa ttccccccgg tacgattgtc
    15361 ggcatgagcc cgtgggttat aaatcggcac aaaccgactt ttggcgagga cgtgcatcag
    15421 tggcggcctg aacgatggct tggtcacagc gacacacggc tacaggagct caagaatacc
    15481 attttgacgg ttcgtctgtt ccctgctaca attgcaacaa tgtgcaaagt gcttacaaac
    15541 actggataaa gtttggatat ggacgccgtg tctgcctggg aaagaacatt gcgattatgg
    15601 agatcaaaaa gctcatttca tctctagtgc tgacttatga ggtatgcgta tccatcttca
    15661 acttcgactc gtcgagtact aataacgttt aagtggacag tcatcgaccc aagtgaatat
    15721 cgggtagaga ataagtggtt tttcaagcag agtggcttcg atgttacagt taagcaccgg
    15781 tcatctgtac ggcacacccc ccgggccacc aatatgacga aagttccgcc gaccctggcc
    15841 attccagctt cgtcatccac tgtcgaggtc agagtaataa atacgtacgt atacgaagat
    15901 aatataatgt ccctgctcac taattaatga tcgctaatag acgtaccacc atgcgcaccg
    15961 accactccct gctgtggaag tctccagttg aagggttcaa aggactggat ctgcccatct
    16021 atgcttttct aatatccaac ggtaatcggc acattatttt cgatttgggt ttacgccagg
    16081 attatgaaaa tctccctcct cgcattgctg gtctgctcaa gaacgcacca tacattgtga
    16141 ccgaagccaa cgtatcggag attctggact cagatgacac cggactagac attaaagggc
    16201 gggacatcga ggcagtaatc tggtcacatc accattacga tcacactggc gatcccagta
    16261 cctttcctcc atctaccaag ttagttgtcg ggcccggggt cctcagtctt acgggtgggg
    16321 gataccccaa gaaccctaac acgaccgtcc tagagaccga cctctccggc cgcaagatcc
    16381 aagagatatc ctttgacgca caagccgaca gtagtgtgaa ggtcggtcca ttcgatggcg
    16441 tcgactactt tggcgatgga tctttctacc ttctgaatgc acctggccat tctgtcgggc
    16501 atatgtgcgg ccttgcccgt gtgacaactg caccagatac gttcatcttc atggctgcgg
    16561 atggatgcca ccatcctggg gccattcgac cctcggagta catagctctc ccccgcgaca
    16621 ttcccaagag cctcgtcagg aagttacgta ctgcagaggc agattctgga ggaaaggcgc
    16681 aagatggtga tacgaagcct ctactcccat ttcttcctgc tttatttcca gattatactc
    16741 aggcgatgga gacggtcgag aagattaaac agcttgatgc ttgtgacaat gtgtttgtga
    16801 ttttgcctca tgatgggagc ttacttgggg ccattgactt tttcccgcgc ccgatcaacg
    16861 actggaagaa gaaggggctg aaagaaagta ccaggtggaa attctgtcag gagatggagg
    16921 aggctttgtc tggatagaca gttggtaaac tggacaattc gcaactccaa cttggtttca
    16981 ttggaaatgc ccatgcagcg acactatttg ctacactact gaacatcttc cctcccatcc
    17041 tgccagctcg agcagttcac gcaacgcaaa gccttttcaa ggtcaatatt gagcattgta
    17101 cgaaagaaaa ctgacatact ctctcgctta tccacttccg acagtacttg cacttgacag
    17161 tccgttttat aatcttcctt ttcgagaaca ccatgtagag ctgagccaaa gtatacaaag
    17221 ttaggctgat cacaagaaaa ttcacaattt tctccagaaa gacactatca atgcttagta
    17281 ttgacgattt gatgcacgaa gaaagccaga aaactcaccc ataagccaaa acaagcgcac
    17341 cgtcatctct cgcctgctct attgtgttgt agccctcttg ataatgcggt ccctttgaca
    17401 gaatagcgaa tttctcatcc atattcctca tgataaaggg gagcaacgag acaagaggaa
    17461 ggacaatgtc cgacacgaac gagttgacaa cgcttgtaaa tgcttgtgcg atactgcaat
    17521 atatcatcag cgagaccttc tattgtaggg tatctggttc catacatcaa acccagggcc
    17581 acctccagga cattatcgcg ggcggcaaaa tcgatgaaac catcccaggc gcggtaaaca
    17641 cggtctttgg catcgctgcc taggcgcagg gcagtttctg tgctctcccc aagcccgcgc
    17701 atagctcttt ggcctttgaa gagagagaaa ggagagagaa aacctggtgt ccaggtattg
    17761 aggagatatg acgttggtgg cctcatgacc ggcgtccatt gtgtcgatca gcagcgccaa
    17821 gcgttgtgct tgtttcgggt tggatgggct gaaacttgaa ttgttttagt attcgagctt
    17881 gaagtaaaac aagatctagt gtattcatta taatattgta atcgagtcaa ctgtagttta
    17941 aactactcgg agggtcccgg gagaagtgtt gcctagatat cggagatata tattggtgta
    18001 cttcgtatgt ttctacagat ccgcaggaaa tggactacgc cagaacgaat acagcccggt
    18061 aattccacct atgtcttgcc agagagacac cttccaagaa gaaataagtc caaccgtgtc
    18121 cttgcccaaa atcagctgta caaagtaaat caagacccac gccaccatac tcagtagctg
    18181 cctcgactac tctgtgagtc acgtgctgca aaaggaccat taatcccgat tcggctagcg
    18241 tcgtcggtag cgcgggagct ttggggccag tcccgtgact ccgtcttcgc gttgcttgtt
    18301 ttgtggattt gtggatttgt ggatagcaat cgggatgctt cttctttgct tgtcgaggcg
    18361 gtcatccctc atgccagcgt gtattgcctt ccttcagcca gccacggaaa tttccttcgc
    18421 agcccattac tgccctagct cacgcaacca atgctcaacc aagaccagcc cacattcgag
    18481 cgaaaatgat ccacataccg tgattttgct acatatacct tacgcaaggg acaccattcc
    18541 tcactgtgaa ctgcaagcaa tattcttggt atgcaagggt agcccttgcc gttcggacgc
    18601 gggctcttcc gcacatacta cggctgaatg gcagtcgctc gctgtgaata gttgccagcc
    18661 tctgctttgc ctctgcaaca tagaccgtgg atcgcatcct cgggcttccc aatgtcatgc
    18721 aaaccagccc tcatagagcc ggaaattact gatggggccg agaccttgca tgacgacctt
    18781 acgtctcatt ctgcagcggt ggacccataa gtcgtcaacc gcaaggtaag attaagttgt
    18841 agagatcgtg attacattgc gacagcccag accccaaaaa gggtcctatg gaaatgacaa
    18901 ccggccagcg aggagctaga cgactttcct ttcggcatag ctgccacgta ctcgtgcggc
    18961 atgtcgcacc gccattcttc tctcagcggc aaggagtaag gacttgttaa caaaaggcag
    19021 gatgaatagg cggtctacaa ttggctgttg gatatctatg acccttcttt caccagcatc
    19081 ctacgggcca gcagctcttt ccctgctctt cgtctgtccg agagtgtcgt ttcttcaacc
    19141 gcgtctccaa caattcaaca ttgaacattt gtttgcattt tcaggaacgc gcttttttct
    19201 caactttgcc gattgcaatg gaagccagcc aggggattgc cgttctgttt gggcctcagt
    19261 ctgccgatat cgaggaagcc gtttcctgca tccaggcgtt tgttcgagag aacccatccg
    19321 cgacctacct tattgacata atacaaagtt taccttctat atggccagcc atacaggact
    19381 cttgggtacc attggcccag atcgatggta aacgccaact tgagacgtta ggaagccttt
    19441 ttgggggagg ggctgccacg gttccgccca aatcatcgaa cattctgata acgcctctta
    19501 ccgtgctaca acatatcgtc gagctttgca gattaaggaa acgaggaagc agtctgcaaa
    19561 taaaggatgt ccagggtttc tgtgtcggct ttcttgcggc cacagcggtg gcttcggctc
    19621 atgatgagac gcaatttcgt tcgattgtgg ccaaagttat ccgtcttgcg gtatgcgtcg
    19681 gcgctctcgt cgatttgaat gagctcgagc gtggccgtgc tgcctctctt gctgttaggt
    19741 ggagcggcga ggagggttat tcctgtatgg agaaggtgct tgctgcgcac cccgaggtaa
    19801 gccattccag tctgaccctc tagagacctg gtttgctgac tccacggtct aggcatacat
    19861 ttcatgcctg accgatacca accgtgcaac gataacagtc ccgcaagagc tacaacggga
    19921 catcatccgc gagatggcga accagggatt atcggtccgg tcaattcctc tctgcggacg
    19981 attccaccac ccagaccatg ccccagccgt gcgacagttg atgcaactgt gcaggtccga
    20041 tgaccggttc cagctgccgg actcaaaaac tctgcaattc ccgctgaggt caaacatcga
    20101 cgccgaactg gttccagaag ggccgctcca cgatattgcc ctccagtcaa tcctgtgctt
    20161 gcagtcaaaa tggcatgcta ccgtctctgc ggcgctggag agcctgacag agagaaacgg
    20221 aaacgtgccg atagctgcaa ttgggcctga gcagtgcttg cctcgaacgg cgagaagtcg
    20281 cctccgacag acctggggca gcaccctcgt cccgatgggt actgggcaca ccgtcaatgg
    20341 gaacctgaca ccgagtcggg ccacatcacc ggcggactcc agcagcgagc taatccctac
    20401 tgttcagccg atagctatca caggaatgtc ctgtcgctac ccgcaggcag actcggtaga
    20461 ggctttatgg gagcttttgg aactgggtaa atgtgcagtt ggccctctgc aaaacaagcg
    20521 ctttagaatg gatgaacttt tgcgtgaacc aaatggtcca ttctggggca actatctgga
    20581 agagcccgat gtttttgacc atcgcttctt tggtatctct gctcgggagg ctgccaccat
    20641 ggatccacag caaagactgc tcttgcaggt tgcctacgaa gcgatggagt cagctgggta
    20701 ttgcggtatc cgcagcagcc aggttcctga ggatgtagga tgctatattg gcgttggaag
    20761 cgacgattat accgataatg taggatcaac tcacgcgaat gcctactctg caccaggcac
    20821 tctgcaagcc ttcaatacgg gccgcatcag ccaccatttt ggctggagtg gaccttcggt
    20881 tgtggttgac accgcttgct catcagcggc tgttgcaatt catatggcct gtcaggtaaa
    20941 tacaccccgt ctctctctcg ctaacgcata ctaacccttg accccagggt cttcataccg
    21001 gtgattgctc gattgcggtt gcaggtggag tgaacgtcat gacgagccca aaggtgaccc
    21061 agaacctggc cgcagcttct ttcctctcgc caacgggggc atcaaaggca ttcgatgcga
    21121 gtgccgatgg atactgtcgt ggtgaaggag cagggctagt cgttctgcgt cctttggatc
    21181 aagcactcca gaatggagac cccgtcctgg gcatcattac tggcactgct gtcaaccaag
    21241 gatccaactg ctcacccatc actgtcccgg tatccaggtc ccaaatgtca ctctacagga
    21301 aggcgctttc agcctctgga gtgtcccccg acgacgtgac atatgttgag gcgcacggca
    21361 ctggtaagtc accttgagca ttatttgaca gttgcgtgct aacaacggct caggaacgca
    21421 agttggtgat ccgattgaaa tggatagcat tcgcaacaca tttggaggta cacatcggac
    21481 cgagcaactc tatgtcgggt caatcaagga caatgtcggg cacacagaaa cctcctctgg
    21541 tgttgccggc ctgattaaga caattctcat gctgcagaag caaaccatcc ctaagcaggc
    21601 gaacttctcg cggctcaacc ccaagatccc agcactcgat atggacaatg tagtcattcc
    21661 cacccggtcc accgactgga aagtatcgac gcgtgttgcc atggtcacga attatggtgc
    21721 cgctggctcc aatgcatcca tcatagttcg acaaccattc acgtacccag gcggcacgcg
    21781 aacgacccta tcccatgccc caatcttcat tgcggctaaa acagcagagt ctctgcgcac
    21841 ctactgtgac aatctcctgg cctctctccg ccaaatcccc gtcctgcctg atcacctagt
    21901 aagcgactat gcgtacaacc tagccaccaa acagaatcgc accatggatt gctatatcgg
    21961 ctttgctacc gacagtccag cctccctcac gaaccagctg gctgcggtgg catctggtgc
    22021 ccgggaagtg tactctcgat ctggaaaggc actgccggtt gtcctatgct tcgggggcca
    22081 gaacggtaac attgtccaca tctcccaaga cctttatacg aacacccatc tgttgcagtt
    22141 ccatctcgcg gactgtgaaa aggcctgcca gtccctgggg cttccaagcc tcttcccaac
    22201 cattttcagg gcagacccta ttgaggatct agttagcctt cactccgccc tttttgcaac
    22261 ccagtatgcc acggccaagt gctggattga ctctgggctt gaggtggacc gcatgattgg
    22321 gcatagtttt ggtcagctta ctgcgttatg cgttgctggc ggcttgagtc tggtcgacgc
    22381 gcttcgcttc atttctgaaa gagctcacct tctcgagaaa cactgttctg ggaagcgtgg
    22441 tctaatgttg tctgtggaag ccagcgaaga ggacgttcgc ggcctagtta atggcgctgg
    22501 tcaggctgtg gatgtgactt gttacaatgg accaagaagc ttcgttattg gtggcgaaaa
    22561 ggttgcgatt gaagcttttg agaaggtggc cactggcttc aagacacagc gtctggccaa
    22621 cacgcatgct tttcactctc acctgatgga ccctattctg cctgctctgc gtgaagtagc
    22681 acagtccatg actttccagc ccaccacaat cccagtagag gcatgctctc aggatatgga
    22741 atggaccttc gtccatgcgg cgcagctggt aactcatact cgccagccag tctactttga
    22801 atctgcggtt cgtcgcatcg ccaacagata tccaacaggt gctgtttggc tcgaggcagg
    22861 ttccgcctcg cccgtcattc caatgattcg tcgcgtggta gagtcctcgc caaacaatca
    22921 cgtatatcag cctattgata ttagacctcc ccaagcgatc gccaacctca gcaaagcgac
    22981 aagcgggttg tggtctaatg gtacgcacgt gcagttctgg cccttccaca agtctcagtc
    23041 caactgctat aactcgatca acctcccgcc ctatcaattc accaaaaccc gtcactggat
    23101 tgactatgat ccgttagcct tcatgccttc gtcggccccc aaggacgcgg gccccgagga
    23161 agcaaaaaag ttggtgcaac tgctgaagca acagtcagga gaatgtctgt ttacaatcaa
    23221 cacgaaggat ctcctgtatc aaacctgcac caagggtcat gcagttgtca accagaatct
    23281 ttgccctgcc tctttgtatc ttgagatggt tatcagcgct gccagccatg tctgccccgt
    23341 cgagctttca tcaaccatgc ctcatgttca gaatctctgc atcttggctc cgcttgttct
    23401 caacccccag ggcgaactgc agctacgtct atcccaacag aacggtgaaa aggatcagtg
    23461 gacattctcc ctatttaccc aacagggaca aacatccacc gttcacgcga cgggaaccat
    23521 ctggctccat gcaatcacca attcctctgc cattgtcagc cgattccgat cccttaaccg
    23581 tttgatgatt ccttctcgac cggactcaat tgaaaactct cctcggtcaa gtgggttgaa
    23641 gggagctgca atctaccaat ccttccgcag agtggtgaac tacgccgaat actatcgcgg
    23701 cgtggagaac gtgtttgccc tgaccaacga ggccactgga caggtcaaaa tcgacacagc
    23761 acttgtcaag catagctgct gtgatccgat cctgatcgac aactttatcc aggtcgccgg
    23821 catccatgta aattgcttgt ctgagatgtc cgaggatgaa gtgtacgttt gcagtgccgt
    23881 cggcgaaatc ttcatcggcg aagcctttat gcaacgtgat tctggcgcgg cgctctcctg
    23941 gagggtgtat tccaactatg accggctgtc caggaaccaa gtcgcctgcg acgtgtttgt
    24001 catgaatcag gaatcagggc agcttgctat cgcgatcatg ggggctacct ttactggggt
    24061 ctctatccgc gctctcactc gcaccttagc caggctcaac aatcagcagt ccagtgcacc
    24121 tgaagttcac gatgccccaa tccctcaaga tactgtgaga gccgatgcaa tgcctgctgc
    24181 tacccccgct cccttgccct caactttaca agaggtcgac aatctccccg ccgtacaggg
    24241 aatgttgggt gatctacttg gtgtcgggct agaagagctt gcaccctctt gctctttgct
    24301 ggagatcggc gttgactccc tgatgagcac cgaagtcctg actgaaatca agaagcggtt
    24361 caatgtcaac atcacgagtg ctgccttgag cgagattccc gacatcggcg ggctcgtcca
    24421 ggtcatattc cctggcacat ctgtcagtca gaccacagtc tcgcaaaccg caccaaacga
    24481 cagtaatgcg gttgtggtag agagtacacc tgttggagaa ttatcgatgc ttgtgcaaga
    24541 agcctacgat gccttcgacg caattcggtc aatgacagac tatagtcgcg aaaccaagtg
    24601 gacaggcttc tacgacatcg tattccctaa gcagatggcg ctggtcacgg cctatgtggt
    24661 cgaagccttc caagcattgg gacatccctt gcagtcgatt agcgcgggag gagaagtccc
    24721 tcttattcca gttcttccac agcacgagaa ggtgcgcaac caattctacg cagtactcga
    24781 attctcccgc ctcgtttcac gcacgactga tggtcgcatc gttcgtacta aagaacaaat
    24841 tccgaccgaa gctgcttcaa acctgcacga cgagatcatc cgtcagttcc cccatcacgc
    24901 gtcagagcat atgcttctac gtaccactgg atcccagctg gctgagtgcc tgagtggcga
    24961 tgccaacccg ttggctctgc tcttccaaga cgcagaagct cgtcgactca tgggcgatgt
    25021 gtataccaat gccccgatgt tcaaatccgc aaccatgcac ctggcgggtt atcttcaagg
    25081 tgtactcgag cgcgttgggt ccggaaggac tgtcaagata ctcgagatag gagcgggaac
    25141 aggaggcacg acgagatacc tggtgagcca actggctgcg cggggtctcc ggttcgaata
    25201 cacctttacc gacatctcat cgtctctagt catgctagca aagaagggat tcaaagagta
    25261 cgattttatg cgctacatga ctttgaatat cgagcaggac cctccgcagg agttgctggg
    25321 acagtatgat attatcattt cgaccaattg cattcatgcg actcgcaaca ttgcgtactc
    25381 atgtagcaat atccgaagaa tgctgagacc ggacgggatt ctgtgcctca ttgaactcac
    25441 ccggaatctg ttctggtttg acctcgtgtt tggcctcctg gaaggatggt ggttgttcga
    25501 cgatggacgg aagcatgccc ttgccagcga gcatctttgg aatgagactc tagacaaggc
    25561 cgggtacagc tggattgact ggtcccgcaa cgaattgaag gagtcagaat tcctacgcct
    25621 tatcgtcgcc tccccgagca agcccgagat gcccctggtc acgcaagaga ccgtcaagtt
    25681 tgatgagaag gatggcgtct ctctactggc tgatatatac tatccctccg ttgcagacga
    25741 tgcgaaccaa cgacgaccca tcggtgagtc agcctagact gacttcgaac gttatattga
    25801 tcaattggca gccctactcc tccacggtgg cggccacatc atgctgtctc ggaaggatat
    25861 ccgcgccgac cagaccatgc tactccttga cgcagggttc ctccctgtga gcattgacta
    25921 ccggctctgc cctgaaacat ctctcataga gggacccatg cgcgatgctc gtgacgcgct
    25981 ccgttgggct cgtcaaactc tccccaccct gccattgcag cgtgaagaca tccaccccaa
    26041 cggcgaccaa gtcgttgcaa taggctggtc aacaggcggc catctagcca tgagtcttgc
    26101 ctggaccgct gcagaactcg cagttgcagc cccagaggca gttctctcat tctactgccc
    26161 aacagactac accgatccgt tctggtccca gcccaacttc cccttcggca agcaaatcgc
    26221 cccccctggg gaaattgata tccgagctgg gatgagtccg actcccatca ccgcgtataa
    26281 tccaccgcgc agcaaaaggg cactcggcgg gtggatgtct actaccgacc ctcgcagcca
    26341 gattgcgctt cacatgaact ggaagggcca aacgctccct gttctcctca acgcggcgca
    26401 aacggcaccc ggcgagaagc tcctggatcc gacgaaagag gatattgtgc gcgtttgtcc
    26461 gacatctcaa gcttctgcgg gcaattaccg gtcccctacg tttatcattc acgggtcttt
    26521 ggatgacttg attccgatga gtcaggttag gcatacggcc aaggtaatga aggctaatgg
    26581 cgttgatgtg cagttgaggg agttggatgg ttctattcat ctctttgata ttgcgtctac
    26641 ctatggggat aagccagatg aggttcaggc tgttgctgat ggaattcagt ttttgaaaga
    26701 ccgtgtacaa tgctgatttt ttgctctttg ttctctttcg acaagttgtt gcgagaaacg
    26761 cgtggaccta cctagacaca gggatagtca tcttatatta gatttccctt ttatgc
//
