BGC0002540 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002540 (Streptomyces hygrospinosus subsp. beijingensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS-like	1	108,563	High	tetramycin B	PKS

BGC0002540 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 108,563 nt. (total: 108,563 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 108,564
Label		BGC0002540

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

tetrG: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

tetrJ: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

tetrK: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: aminotransferase glycosyltransferase

tetrMII: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

tetrMIII: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

Lyases: dehydratase

tetrMI: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

tetrA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tetrB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tetrC: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tetrD: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

tetrE: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002540.3	1.26	PKS	tetramycin B	Streptomyces hygrospinosus subsp. beijingensis
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001164.3	0.58	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.58	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000138.5	0.56	PKS	rimocidin	Streptomyces diastaticus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002540.3	1.00	PKS	tetramycin B	Streptomyces hygrospinosus subsp. beijingensis
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 108563:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - ohmal - Me-ohmal - ohmal - ohmal - ohmal) + (ccmal) + (redmal) + (ohmal - ccmal - ccmal - ccmal) + (X - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

tetrC: mal - mal - mmal - mal - mal - mal

PKS_AT (558..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.8
inactive: 88.5
Methoxymalonyl-CoA: 52.5
Methylmalonyl-CoA: 44.7
Propionyl-CoA: 28.2
Acetyl-CoA: 25.8
Benzoyl-CoA: 17.6
CHC-CoA: 16.6
Isobutyryl-CoA: 15.4
Ethylmalonyl-CoA: 15.3
trans-1,2-CPDA: 10.5
2-Methylbutyryl-CoA: 10.4
fatty_acid: 6.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (2322..2620): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 196.1
inactive: 83.4
Methoxymalonyl-CoA: 68.9
Methylmalonyl-CoA: 57.5
Isobutyryl-CoA: 31.2
Propionyl-CoA: 29.4
Ethylmalonyl-CoA: 21.2
Acetyl-CoA: 21.1
CHC-CoA: 18.4
Benzoyl-CoA: 17.3
2-Methylbutyryl-CoA: 11.9
trans-1,2-CPDA: 8.8
fatty_acid: 8.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (3863..4160): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 217.9
Ethylmalonyl-CoA: 202.4
Methoxymalonyl-CoA: 178.1
2-Methylbutyryl-CoA: 144.6
trans-1,2-CPDA: 131.8
Propionyl-CoA: 111.2
CHC-CoA: 111.2
Malonyl-CoA: 88.4
Isobutyryl-CoA: 83.6
Benzoyl-CoA: 64.9
Acetyl-CoA: 43.6
fatty_acid: 42.1
3-Methylbutyryl-CoA: 41.9
inactive: 19.0

PKS_AT (5396..5696): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.9
inactive: 108.3
Methoxymalonyl-CoA: 76.7
Methylmalonyl-CoA: 66.8
Isobutyryl-CoA: 49.7
Propionyl-CoA: 29.2
Ethylmalonyl-CoA: 26.6
Acetyl-CoA: 25.9
fatty_acid: 22.5
Benzoyl-CoA: 22.4
2-Methylbutyryl-CoA: 21.8
3-Methylbutyryl-CoA: 17.0
trans-1,2-CPDA: 13.6
CHC-CoA: 11.1

PKS_AT (6916..7201): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 237.7
inactive: 114.8
Methoxymalonyl-CoA: 61.3
Methylmalonyl-CoA: 58.9
Propionyl-CoA: 26.7
Benzoyl-CoA: 20.3
Acetyl-CoA: 16.6
Isobutyryl-CoA: 16.4
2-Methylbutyryl-CoA: 11.5
Ethylmalonyl-CoA: 8.7
CHC-CoA: 8.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (8427..8720): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 235.3
inactive: 113.0
Methoxymalonyl-CoA: 71.9
Methylmalonyl-CoA: 66.2
Isobutyryl-CoA: 44.7
Ethylmalonyl-CoA: 35.0
Propionyl-CoA: 29.8
Benzoyl-CoA: 25.5
2-Methylbutyryl-CoA: 16.1
fatty_acid: 13.4
CHC-CoA: 12.0
trans-1,2-CPDA: 8.1
3-Methylbutyryl-CoA: 0.0
Acetyl-CoA: -0.4

tetrD: mal

PKS_AT (551..838): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 237.3
inactive: 116.1
Methoxymalonyl-CoA: 72.8
Methylmalonyl-CoA: 66.5
Propionyl-CoA: 31.4
Isobutyryl-CoA: 25.1
Acetyl-CoA: 22.2
Ethylmalonyl-CoA: 17.7
Benzoyl-CoA: 17.4
2-Methylbutyryl-CoA: 10.4
CHC-CoA: 10.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

tetrE: mal

PKS_AT (552..837): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.7
inactive: 107.3
Methoxymalonyl-CoA: 70.4
Methylmalonyl-CoA: 57.2
Propionyl-CoA: 25.7
Acetyl-CoA: 24.0
Isobutyryl-CoA: 21.4
Ethylmalonyl-CoA: 19.0
Benzoyl-CoA: 16.1
CHC-CoA: 11.9
2-Methylbutyryl-CoA: 9.0
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

tetrB: mal - mal - mal - mal

PKS_AT (571..869): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Ethylmalonyl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 150.5
Methylmalonyl-CoA: 64.6
Methoxymalonyl-CoA: 64.5
inactive: 55.4
Ethylmalonyl-CoA: 29.2
Isobutyryl-CoA: 27.8
Propionyl-CoA: 26.4
trans-1,2-CPDA: 22.6
Benzoyl-CoA: 13.0
Acetyl-CoA: 12.8
CHC-CoA: 5.4
2-Methylbutyryl-CoA: 4.2
3-Methylbutyryl-CoA: 3.9
fatty_acid: 0.0

PKS_AT (2106..2396): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.5
inactive: 110.4
Methoxymalonyl-CoA: 58.3
Methylmalonyl-CoA: 49.7
Propionyl-CoA: 26.6
Benzoyl-CoA: 17.2
Ethylmalonyl-CoA: 15.9
Acetyl-CoA: 13.4
CHC-CoA: 12.9
2-Methylbutyryl-CoA: 9.8
trans-1,2-CPDA: 5.2
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3831..4120): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 230.0
inactive: 107.7
Methoxymalonyl-CoA: 48.3
Methylmalonyl-CoA: 39.8
Propionyl-CoA: 29.2
Acetyl-CoA: 20.9
Benzoyl-CoA: 13.0
2-Methylbutyryl-CoA: 11.2
Isobutyryl-CoA: 10.4
fatty_acid: 9.2
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -4.5

PKS_AT (5531..5821): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.8
inactive: 111.7
Methoxymalonyl-CoA: 49.4
Methylmalonyl-CoA: 40.7
Acetyl-CoA: 24.5
Propionyl-CoA: 19.3
Benzoyl-CoA: 18.2
Ethylmalonyl-CoA: 14.8
2-Methylbutyryl-CoA: 12.4
Isobutyryl-CoA: 8.6
CHC-CoA: 8.5
fatty_acid: 8.1
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

tetrA: X - mal

AMP-binding (29..437): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LFQFRAVTGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

PKS_AT (1188..1489): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 180.8
inactive: 69.0
Methoxymalonyl-CoA: 67.8
Methylmalonyl-CoA: 59.1
Ethylmalonyl-CoA: 38.0
Propionyl-CoA: 36.5
Isobutyryl-CoA: 35.7
Acetyl-CoA: 35.5
CHC-CoA: 23.0
Benzoyl-CoA: 22.5
fatty_acid: 22.0
2-Methylbutyryl-CoA: 21.5
trans-1,2-CPDA: 8.5
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor