BGC0002542 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002542 (Moorena bouillonii bAprat14)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,PKS-like,NRPS	1	37,851	apratoxin A	PKS+NRPS:Type I	100%

BGC0002542 - Region 1 - NRPS,PKS-like,T1PKS

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Location: 1 - 37,851 nt. (total: 37,851 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 37,852
Label		BGC0002542

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002542	2.64	Polyketide, NRP	apratoxin A	Moorena bouillonii bAprat14
BGC0001164	0.92	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001163	0.83	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001064	0.82	Polyketide	cylindrocyclophane D, cylindrocyclophane E, cylindrocyclophane F	Cylindrospermum licheniforme UTEX B 2014
BGC0001357	0.78	Polyketide	carbamidocyclophane A, carbamidocyclophane B, carbamidocyclophane C, carbamidocyclophane D, carbamidocyclophane E, carbamidocyclophane F, carbamidocyclophane H, carbamidocyclophane M, carbamidocyclophane N, carbamidocyclophane O, carbamidocyclophane P, carbamidocyclophane Q, carbamidocyclophane R, carbamidocyclophane S, carbamidocyclophane T, carbamidocyclophane U	Nostoc sp. CAVN2
BGC0000205	0.77	Polyketide	bryostatin	Candidatus Endobugula sertula
BGC0001566	0.76	Polyketide	cylindrocyclophane	Cylindrospermum licheniforme UTEX B 2014
BGC0001162	0.74	Polyketide	1-heptadecene	Moorea producens JHB
BGC0001187	0.74	NRP, Polyketide	xenolozoyenone	Glarea lozoyensis
BGC0002412	0.74	NRP	holomycin	Photobacterium galatheae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002542	1.00	Polyketide, NRP	apratoxin A	Moorena bouillonii bAprat14
BGC0001164	0.69	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001163	0.66	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001909	0.62	Polyketide	strobilurin A	Strobilurus tenacellus
BGC0001064	0.62	Polyketide	cylindrocyclophane D, cylindrocyclophane E, cylindrocyclophane F	Cylindrospermum licheniforme UTEX B 2014
BGC0001162	0.60	Polyketide	1-heptadecene	Moorea producens JHB
BGC0001357	0.60	Polyketide	carbamidocyclophane A, carbamidocyclophane B, carbamidocyclophane C, carbamidocyclophane D, carbamidocyclophane E, carbamidocyclophane F, carbamidocyclophane H, carbamidocyclophane M, carbamidocyclophane N, carbamidocyclophane O, carbamidocyclophane P, carbamidocyclophane Q, carbamidocyclophane R, carbamidocyclophane S, carbamidocyclophane T, carbamidocyclophane U	Nostoc sp. CAVN2
BGC0001566	0.59	Polyketide	cylindrocyclophane	Cylindrospermum licheniforme UTEX B 2014
BGC0001161	0.59	Polyketide	1-heptadecene	Moorea bouillonii PNG
BGC0000205	0.59	Polyketide	bryostatin	Candidatus Endobugula sertula

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 37851:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal - mal) + (Me-ccmal) + (Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

aprB

PKS_AT (549..854): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 100.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 223.4
inactive: 212.1
Methoxymalonyl-CoA: 71.9
Methylmalonyl-CoA: 67.1
Propionyl-CoA: 42.0
Isobutyryl-CoA: 29.3
Benzoyl-CoA: 28.8
2-Methylbutyryl-CoA: 22.8
Ethylmalonyl-CoA: 18.4
Acetyl-CoA: 17.2
CHC-CoA: 16.2
trans-1,2-CPDA: 12.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2255..2553): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 100.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.4
inactive: 191.5
Methoxymalonyl-CoA: 60.8
Methylmalonyl-CoA: 56.6
Propionyl-CoA: 36.9
Isobutyryl-CoA: 32.4
Benzoyl-CoA: 19.3
Ethylmalonyl-CoA: 18.4
Acetyl-CoA: 16.6
2-Methylbutyryl-CoA: 14.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

aprG

PKS_AT (1271..1569): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 100.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.8
inactive: 204.4
Methoxymalonyl-CoA: 70.5
Methylmalonyl-CoA: 65.7
Propionyl-CoA: 41.6
Isobutyryl-CoA: 39.0
2-Methylbutyryl-CoA: 25.5
Ethylmalonyl-CoA: 21.3
Benzoyl-CoA: 19.1
CHC-CoA: 16.5
trans-1,2-CPDA: 13.6
3-Methylbutyryl-CoA: 12.9
Acetyl-CoA: 12.4
fatty_acid: 0.0

aprH

Search NORINE for peptide: strict or relaxed

AMP-binding (544..943): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSLIWK
Nearest Stachelhaus code(s):: Cys DLYNMSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)