LOCUS       BGC0002551             13360 bp    DNA     linear   BCT 07-NOV-2019
DEFINITION  Nostoc sp. UHCC-0398 muscoride biosynthetic gene cluster, complete
            sequence.
ACCESSION   BGC0002551
VERSION     BGC0002551
KEYWORDS    .
SOURCE      Nostoc sp. UHCC-0398
  ORGANISM  Nostoc sp. UHCC-0398
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae;
            Nostoc.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:58:44
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13360
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..13360
                     /db_xref="taxon:2665154"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Nostoc sp. UHCC-0398"
                     /strain="UHCC-0398"
     protocluster    1..13360
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3018:6780]"
                     /cutoff="20000"
                     /detection_rule="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /neighbourhood="10000"
                     /product="cyanobactin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3019..6780
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /neighbourhood="10000"
                     /product="cyanobactin"
                     /protocluster_number="1"
     cand_cluster    1..13360
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="neighbouring"
                     /product="cyanobactin"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     subregion       1..13360
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002551"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..13360
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /contig_edge="True"
                     /product="cyanobactin"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(211..411)
                     /gene="musC2"
     CDS             complement(211..411)
                     /codon_start=1
                     /gene="musC2"
                     /product="MusC2"
                     /protein_id="QGA72593.1"
                     /transl_table=11
                     /translation="MAKKQQTPENSEPTPKKQPEGIPKQVESHLLATGLEDYGFWWQEM
                     LKAQANSDKERKPFRRGRIWS"
     misc_feature    211..13006
                     /note="muscoride biosynthetic gene cluster"
     gene            complement(482..1384)
                     /gene="musF2"
     CDS             complement(482..1384)
                     /codon_start=1
                     /gene="musF2"
                     /note="muscoride prenyltransferase"
                     /product="MusF2"
                     /protein_id="QGA72594.1"
                     /transl_table=11
                     /translation="MVTSNPIQTNSEQYLEYIGKHKLAFDIYEDLYPLKLFEDFVEVEA
                     KKRGLFYILSNVDKDEIYPARFCLRFPSLEEAQLLYNPQQQLQTALNFFRQVESRPEVK
                     LNYHHIQQFFGTISDFQGIVLMAVAIDARTVITESRLKLYIWLKNAPEKVETAIALCGD
                     SPTLRAFLVNDQLQVGFDLFFNGESEIEVYPIISQDELQQFHIRDRIIPLLPPRALPLL
                     QQCAVFQVGFSEANESNILYFDYVHDPNSFVDNLGNEMTKKIHAYYRHQPIKSLTVGIP
                     EHNFYGRAIEHVKLYYDMN"
     gene            complement(1440..2342)
                     /gene="musF1"
     CDS             complement(1440..2342)
                     /codon_start=1
                     /gene="musF1"
                     /note="muscoride prenyltransferase"
                     /product="MusF1"
                     /protein_id="QGA72595.1"
                     /transl_table=11
                     /translation="MVTYKPIQVDSEPYLEYIGKHKRAFDIYEDLYPVKLFEDFVQVEG
                     RQGGMFRCGCNVTKDELSPARFALFFPTQDDLQLLHNPQQQLEAAFDFFRKVEGRAEVK
                     VNYHLIEKFFGKNPDFRGIYFIAVGVDAQTEISESRLKLFIHLHDAPDKIESAIALCGD
                     SPTLRAFLINNDLLVGFDFYLDGRSEIEVYPTIYQEELQRADIQSRILPLLPPRALPLL
                     KQCEVLQIGFSPANKSNILYFDFVRDSNTFIDNLGNEMAKKVHAYYRHQPVKHVLVGVS
                     EKDLYASSIEKVKLYYFKK"
     gene            complement(2778..2978)
                     /gene="musB"
     CDS             complement(2778..2978)
                     /codon_start=1
                     /gene="musB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) triceptide_precursor"
                     /gene_kind="biosynthetic-additional"
                     /product="MusB"
                     /protein_id="QGA72596.1"
                     /sec_met_domain="triceptide_precursor (E-value: 1.0,
                     bitscore: 10.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLPKLAPPVKRPDIIYPHRSVDVINGTVEDLVHIRMDLLHGANY
                     NDPPAFNYSACSQSGYGCGGW"
     protocluster    2932..13360
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[7931:10277]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      7932..10277
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="2"
     cand_cluster    2932..13360
                     /candidate_cluster_number="2"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="2"
                     /tool="antismash"
     gene            complement(3019..4653)
                     /gene="musH"
     CDS             complement(3019..4653)
                     /NRPS_PKS="Domain: oMT (46-213). E-value: 2e-25. Score:
                     80.6. Matches aSDomain: nrpspksdomains_musH_oMT.1"
                     /codon_start=1
                     /gene="musH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 121; E-value: 9.6e-37)"
                     /gene_kind="biosynthetic"
                     /note="methyltransferase"
                     /product="MusH"
                     /protein_id="QGA72597.1"
                     /sec_met_domain="cyanobactin_synth (E-value: 1.4e-155,
                     bitscore: 507.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQFLTSNQTHAPPDNHWQRQRQLYNLARFNQNKDISDSWVRSHNL
                     NLAKQAGIKPGNYILDAGCGAGIPALQIAQEFPGIRIEGITSSQTQAQDARGRVQASGL
                     GDRIQIRVGDFNYLPFPNGVFDVAFFNESIKYSKNLTQVLAEVYRVLRPGGQLYITDLV
                     SQELPLTEQQQRELAKLNQDLICHIYPISELAKAGQQTGFQNIEFGDFTKQLPTGDMRK
                     LWLLNPAANLPVYYGEIKASKGVASQITPSQKLEVPVQSQTSRLVYALGTVGYDFGSQA
                     RRDSFKQLMPGIAIDGTVIPANPYDARQVVDYLTDNPSEAKSLIWTLNLDLTPIYAIEP
                     IEAFADDVYEILQLLLAGQIEPEDSNNYIERASIPARLTNRTVKLFSGQIVPVIEPENI
                     RGMYGWKINSLVDAALETLATEGETLEIQRARVQRTLTSFLNRIYYDLRNWGQTDRDRA
                     LNFAATNAFQAASTFKEAVVAGLELDSIEVYKSAYCRYDSNCWDVKLKFFDPESSRRAR
                     KIFRFTIDVREIMPVTLGEVRSWAAPK"
     aSDomain        complement(4015..4515)
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_musH_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="2.00E-25"
                     /label="musH_oMT.1"
                     /locus_tag="musH"
                     /protein_end="213"
                     /protein_start="46"
                     /score="80.6"
                     /tool="antismash"
                     /translation="LAKQAGIKPGNYILDAGCGAGIPALQIAQEFPGIRIEGITSSQTQ
                     AQDARGRVQASGLGDRIQIRVGDFNYLPFPNGVFDVAFFNESIKYSKNLTQVLAEVYRV
                     LRPGGQLYITDLVSQELPLTEQQQRELAKLNQDLICHIYPISELAKAGQQTGFQNIEFG
                     DFTK"
     gene            complement(4663..6780)
                     /gene="musA"
     CDS             complement(4663..6780)
                     /codon_start=1
                     /gene="musA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 160.9; E-value: 1e-48)"
                     /gene_kind="biosynthetic"
                     /note="n-terminal protease"
                     /product="MusA"
                     /protein_id="QGA72598.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 9.6e-37, bitscore:
                     116.2, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 1.3e-220,
                     bitscore: 722.2, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKVKIIPGLTHLWEQTLGKPEICVAVLDGLVDRSHICFQGADLR
                     EIPGMVESIPNPEGLMHRHGTHVASVIFGQHGSPVEGIAPRCRGLIIPIFSDKGRNLSQ
                     LDLARAIEQAVNSGAHIINLSGGQLADYGQADGWLEKAVALCRENNVLLVAAAGNNGCE
                     CLHVPAALPAVLAVGAMDDGGQPIDFSNWGDTYQTQGILAPGENILGAKAGGGIKEWTG
                     TSFATPIVSGVAALLLSLQQQRGEKPDPQKVRDAILQSAIPCELKNQSDRPRCLVGKLN
                     ILGAMEKLKGGKMSQGQEALTASGCGCGGINKGDSLYPINYMNHSAADSSISPSEVTSS
                     EFDGVMESASTEASTPALASVSSPTPNAISTEAASTPDANVSLPTSADLAAFQNLLQSY
                     ITSQNQPMSYPVASSVTASQAPSDINPETLVYALGTLGYDFGTEARRDSFKQLMPGISI
                     DNTGVPANPYDARQMVDYLAENPSEGKSLIWTLNLELTPIYAIEPKGAFARDVYGVLQE
                     LLSGQIQPEDDPEYIERVSIPGLLTGRTVKLFSGQYIPVIEPQNTRGLYGWKVNTLIGA
                     ALDTIGTQVGESQEDNVRRTLGGFLNRIYYDFRNLGTTSQDRALNFATTNAFQAATTFA
                     QAVAEGMELDTISVERSPFCRKDSDCWDVKLKFFDPENNRRAKKVFRFTIDVSDLIPVT
                     LGEVRTWSSAY"
     gene            7321..7524
                     /gene="musE"
     CDS             7321..7524
                     /codon_start=1
                     /gene="musE"
                     /note="muscoride precursor peptide"
                     /product="MusE"
                     /protein_id="QGA72599.1"
                     /transl_table=11
                     /translation="MKPENQKQTRLQPKLSEPTVRVMPGIQLDLSEEELTNGGVGVGGV
                     NASQYGGGDGGVSPSIPSTSGV"
     gene            7932..10277
                     /gene="musD"
     CDS             7932..10277
                     /codon_start=1
                     /gene="musD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Cyanobactin_D_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Goadsporin_CD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NHLP_CD_RRE"
                     /gene_kind="biosynthetic"
                     /note="cyclodehydratase"
                     /product="MusD"
                     /protein_id="QGA72600.1"
                     /sec_met_domain="Cyanobactin_D_RRE (E-value: 1.2e-31,
                     bitscore: 98.1, seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="Goadsporin_CD_RRE (E-value: 5.3e-18,
                     bitscore: 54.5, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="NHLP_CD_RRE (E-value: 3.6e-22, bitscore:
                     67.8, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 6.3e-95, bitscore: 308.0,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 9.2e-86, bitscore:
                     277.3, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKILQIKPHFRAEIIEPKHVYLLSESSTHALTGELYCQLIPLLNG
                     NYTVDEIINKLQVDPSHIDYALERLQARGYLTEATPQLTPEAVAFWGLLKVEPQVAYQC
                     LQQTQVYVSSVANLPTQSLITALEEVGIKAINWDGELQEFPPHSLLVVLTDDYLQPELN
                     KINQIALKGNQPWLLMKPVGTILWLGPIFQPQITGCWECLAQRLRINREVEASVLRQKN
                     SSLQLSPSQESDSSVQQRNGNKVKSEVIECLPPPPAVIPSTLQTALQLATTEIAKWIVK
                     QGVEDTTPFPTLEGKVITFDQRNLDIQTHILSLRPQCPSCGNPNLLTERAFQPLVLSSR
                     KKQFTSDGGHRAFSPDQTVNRYQHLISPITGVVTSLVRSSDPDDSLLHSYNAIHSFAVA
                     SNIGKLRRYLKHKSGGKGKTDRQSKASGFCEAIERYSGVYQGNEPRISATLAELGDKAI
                     HPAKCSLFSSEQYEYREEFNRRGGVFDWIPQPFDETKVIEWTPVWSLTEQTHKYIPTAY
                     CYYGYPLPEDHEFCRANSNGDATGNTLEEAILQGFFEIVERDSISIWWYNRLKRPRVDL
                     ASFNEPYLLEVQDLYKSKNRDLWVLDVTADLGIPTFVAVSYLKGKAQQTILLGFGTHFD
                     PKIAILRAVTEVNQVGFTCDDAEVNKEFGEMREWFKKATIENQPYLVPDSTVPAKVYQD
                     YQLRWSDDIYQDVMTCVEIAKNAGLETLVLDKTRPDIGLNVVKVIVPEMPHYWLRMGAK
                     RIYDVPVKMGWLSTPLTEEQMNPISVPI"
     gene            10548..11924
                     /gene="musG"
     CDS             10548..11924
                     /codon_start=1
                     /gene="musG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_kind="biosynthetic-additional"
                     /note="oxazoline oxidase"
                     /product="MusG"
                     /protein_id="QGA72601.1"
                     /sec_met_domain="PF00881 (E-value: 2.4e-16, bitscore: 49.7,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSAFTISLAKKIDLIPENERIILKSNTYKLTLSHPQPGLKSAFN
                     HLKHSPATLSDLRQLVVETDGIEGKDKFDKYLQGLTNLGWICHSVFPLATAIPMTGNYQ
                     INYPQIQNWQQQTFTLSRFAYLHQVNGEMVLESPQSQAKIILLDWRATALITKLSQPQT
                     STTLIAGIPDITEELAQQFLSLLLATQMLSSETENSHLMQWEFHDLLFHSRSRKGRHSN
                     PVGGTYRFLDKIEPLPVVKPPMSNKVIELYKPDLEDLAKTDISFTQVLESRRSIREYGE
                     DPITIEQLGELLYRSARIQEVIDKENMGQLSRRPYPGGGAIHELEIYPLINYCEGLEAA
                     LYHYRPDAHELELVSEWSQEVEALVTEACLSNGSQKMPQVLIIITARFNRMFWKYQSMA
                     YAAILKHVGVLYQTLYLVSTAMNLAPCGIGAGNSDIFAKATGIDYYEESSVGEFILGTK
                     "
     gene            12080..13006
                     /gene="musC1"
     CDS             12080..13006
                     /codon_start=1
                     /gene="musC1"
                     /product="MusC1"
                     /protein_id="QGA72602.1"
                     /transl_table=11
                     /translation="MNKQLESLQSILVFDGQDNSINLGKKPEFKIEKKITLEAWVYCQN
                     QRRRTGIISNIFDTSDKESGYGLLLDGKSGVFFALKTTSQKIHYLSSKADSLKLNQWHH
                     LAGTYDGKQIKVYIDGVEKAAKAIANSAINYEPENDLFIGTYKDNNETYPFQGKIAEVR
                     IWKVARTQQQIQNNLYQRLAGNEPGLIGYWALNEGSENVAHDGTTNANHGNINGANWEQ
                     SQVAFTNSQSQELVVIKTLTTEKETTLEVKPVEQKLTQNSEKAKEIEKHLLATGLEDYG
                     FWWQEMLKAQAQKTELDKPFRRGRIWA"
ORIGIN
        1 accgaacagt attggaaaag gatctgaaag tttgtgaatg gatgttgcaa gtcgtcaaag
       61 taggatggcg atcgcactta taattaattt ttaaaagtag ggtgcgttgt cacgcactgc
      121 aacgcaccag ctaaagactc agagttgtaa agaagggctg acaagatgtc caacccacaa
      181 gtagtaatta atttcttgga agtctcttaa tcaagaccaa atccgacccc ggcggaaagg
      241 tttgcgttct ttgtctgaat tggcttgtgc ttttaacatt tcttgccacc agaaaccgta
      301 atcttctaaa ccagtggcta acaaatgaga ttcaacttgt ttgggaattc cttctggttg
      361 tttttttgga gttggttctg agttttctgg ggtttgctgc ttcttagcca tgataattta
      421 ctctcctgat ttgatttttt agtttatttg gatcgacttg ctcttattta aaatcaaaat
      481 attaattcat atcataatat agtttcacat gctcaattgc ccgtccataa aaattgtgtt
      541 cgggaatgcc tactgtcaaa gatttaatag gctgatggcg ataataagca tgaattttct
      601 tagtcatctc attaccgaga ttatcgacaa agctattggg gtcatggaca taatcaaaat
      661 agagaatatt actttcattc gcttcactaa aacctacttg aaaaactgca cattgctgta
      721 gtagcggtaa agcacgaggt ggtaagagtg ggattattcg atcgcgaata tgaaactgct
      781 gcaactcatc ttgactgatg attggataaa cttctatttc gctttcacca ttgaaaaata
      841 aatcaaaacc tacttgcagt tgatcattaa ctaaaaatgc ccgcaatgtt ggactatcac
      901 cacacaaagc aatggctgtt tctacttttt ccggagcatt tttgagccaa atatacagct
      961 ttagacgcga ctcagtaatt actgtacggg catctatggc aacagccatc aaaacaatac
     1021 cttgaaagtc agaaatagta ccaaaaaatt gctgaatatg gtgataattg agcttaactt
     1081 ctggtcggct ttccacttga cgaaaaaagt taagggcggt ttgtagttgt tgttgtggat
     1141 tataaagcag ttgagcttct tctagtgaag gaaatctcaa acagaaccga gccggatata
     1201 tttcatcttt atctacatta gaaagaatgt aaaataaacc tcttttttta gcctctactt
     1261 ctacaaaatc ttcaaacagc ttaagaggat ataaatcttc gtaaatatca aaagcaagtt
     1321 tatgtttacc aatgtattct aagtactgtt cgctattagt ttgaatcgga ttagatgtca
     1381 ccataacttt gatgtttatt gaagtttttg ttacttaatt ttttattttt tgacatacat
     1441 tacttcttaa agtagtacag cttcactttt tcaatagagc tagcgtacaa gtctttttca
     1501 gaaacaccaa ctagtacatg tttgacaggt tgatggcggt aataggcatg gacttttttt
     1561 gccatttcat taccaagatt atcgataaag gtattggaat cgcgaacaaa atcgaaataa
     1621 agaatattac ttttattggc aggactaaag ccgatttgca agacttcaca ctgctttagt
     1681 agtggtaaag cacggggagg taaaagtggt aatatcctac tttggatatc agctcgttgc
     1741 aactcttctt gataaatagt tggataaacc tcaatttcac tgcgtccatc taaataaaag
     1801 tcaaaaccta ctaataaatc attgttgatt aagaatgctc gtaatgttgg actatcacca
     1861 cacaaagcaa tggcagattc tattttgtca ggggcatcat gcaggtgaat aaacaatttt
     1921 aaacgggatt cactaatttc tgtctgtgca tctacaccca cagcaataaa ataaatccct
     1981 cgaaaatcag gatttttccc aaaaaacttt tcaatcagat gataattaac cttgacttct
     2041 gctcgacctt ctactttgcg aaaaaagtca aaagctgctt caagttgctg ttgggggttg
     2101 tgaagcaatt gcaaatcgtc ttgtgtagga aagaaaagag caaaccgagc cggagacaat
     2161 tcatctttag ttacattaca accacagcga aacatacctc cttgcctacc ctctacttgt
     2221 acaaaatcct caaaaagttt aacgggatat aggtcttcgt aaatgtcaaa ggcacgttta
     2281 tgtttaccaa tgtattctaa ataaggttcg ctatcaactt gaatcggttt ataagttacc
     2341 ataatttatt tttttaccct gccgatatgt ataagcttgg gtaatcatta cccaccctag
     2401 aaaatatttt tttagaatca aatatttagt gaaggactta cagcactttg caattaaaca
     2461 aaccacacat ctcaatatga taaatcttgt ggggtgggca tcctgcccgc ccttatttag
     2521 ggaactcaaa aaaatcaatt atccaatatt gtggggtgaa tatgttgttt ttcctatgga
     2581 ctgggcgggc gagacgccca ccccacaaga gagaattgga tattttttat ttgtcagtgc
     2641 tttatctaat ttagatgaga tgtcctgtaa atccatttat tcatttgcta gttccacaga
     2701 attaaattca gaattctgac tcctgaattc tgactcctga ctcctgactc ctgactcctg
     2761 aattctgact cctgaattca ccaaccacca catccatagc cagattgaga acaagcactg
     2821 taattaaagg ctggaggatc gttatagtta gctccgtgta ataaatccat gcgaatgtga
     2881 accaaatctt ctactgtgcc attaataaca tccacggaac ggtggggata aatgatatcc
     2941 ggtcttttca ctggaggagc aagttttggt aatctcatag ttgtccaaag ttaatatttg
     3001 cttgataaat aatctagctt atttgggtgc agcccaagaa cgcacttcgc ccaaggtgac
     3061 aggcatgatc tcgcgtacat caatagtaaa acggaaaatt tttcttgccc gacggctact
     3121 ttctgggtca aaaaacttca atttcacgtc ccaacagttg ctatcatacc gacagtaagc
     3181 gcttttatat acttcaatgc tgtcgagttc caaacccgcc accactgctt ctttaaaggt
     3241 agaagctgct tgaaaagcgt tagtagctgc aaaattcagc gccctatctc tgtctgtttg
     3301 cccccagttt cgcaagtcgt agtagatgcg gttcaagaaa ctagttaaag tcctttgtac
     3361 tcttgctctt tggatttcta gtgtttcacc ttctgttgct aacgtctcta aagcagcatc
     3421 cactaagcta ttaattttcc aaccatacat tccccggata ttttctggtt caatcactgg
     3481 aacaatttgc ccagaaaata gcttaactgt tctatttgtc aatcttgctg ggatacttgc
     3541 ccgttcgatg taattattgc tgtcttctgg ttctatttgt ccggctaaca gcagttgtaa
     3601 gatttcataa acatcatcag caaaagcttc aatgggttca atggcataaa ttggagttaa
     3661 gtccaaattc aacgtccaaa ttaaagattt tgcttccgaa gggttatctg ttagataatc
     3721 taccacctga cgggcatcgt agggattggc tggaattact gtgccatcaa ttgcaattcc
     3781 aggcattaat tgtttaaagg aatctcgccg tgcttgggag ccaaagtcat agcccacagt
     3841 gcccagtgca taaactaaac gactagtctg gctttgaacg ggaacttcca gcttttgact
     3901 tggggtgatt tgagaggcta cacctttact agccttaatt tctccataat aaactggcaa
     3961 attagcagca ggatttagca gccacagttt tctcatatct cctgtgggca attgtttggt
     4021 gaaatcccca aactcaatat tttgaaaacc tgtttgttga cctgcttttg ctaattcact
     4081 aataggataa atatggcaaa ttaaatcctg atttaacttt gccaattctc gctgctgttg
     4141 ctcagttaaa ggcagttctt gactaaccaa atctgttatg tataactgac ctcccggacg
     4201 caacacccgg tagacctctg ctaaaacctg agtgagattt ttggagtatt taatgctttc
     4261 attaaaaaat gcgacatcaa atactccatt ggggaagggc aggtaattaa agtcacctac
     4321 ccgaatctga atgcgatcgc ctaatcccga cgcttgtacc cgccctctag cgtcttgggc
     4381 ttgggtttgg gatgaagtaa ttccttcgat tctgattcct gggaactcct gagcaatttg
     4441 cagcgccgga attcccgccc cacaaccagc atcgagaatg taattccctg gcttgattcc
     4501 ggcttgtttg gctaaattta gattgtgcga tcgcacccaa ctatcgctaa tatctttatt
     4561 ctggttaaag cgtgccaagt tatacaattg acgctgcctt tgccagtggt tatcaggggg
     4621 tgcatgagtt tgattactgg tcaaaaattg catattaaat aattagtaag cactcgacca
     4681 agtgcggact tcgcccaagg taacaggaat taaatcgcta acatcaatgg taaagcggaa
     4741 gacttttttg gctcgacggt tgttttctgg gtcgaagaac ttcaatttca catcccaaca
     4801 atcactatcc ttccgacaga aggggcttct ctcaacgctg atggtatcca attccattcc
     4861 ttccgccaca gcttgagcaa aggttgtggc tgcttggaaa gcattggtgg tggcaaaatt
     4921 caaggcgcgg tcttgagaag tggttcccaa attgcggaaa tcataataaa tccggttgag
     4981 gaagccaccc aaggttctgc gaacattgtc ttcttgcgat tctcccactt gagtgccgat
     5041 tgtatctaag gcagcaccaa ttaaggtgtt gaccttccaa ccatatagcc cccgcgtatt
     5101 ttgtggttca attaccggaa tatattgccc agaaaacagt ttaacggtgc gtccagttaa
     5161 aagtccgggg atgctaacgc gttctatata ttctgggtcg tcttctggtt gaatttgccc
     5221 agaaagcagt tcttgtaaaa ccccgtagac atctctagca aaagctcctt tgggttcaat
     5281 ggcataaatt ggagtgagtt ctaagttcag cgtccaaatt aaagatttac cttccgaggg
     5341 attttctgcc aaataatcaa ccatttggcg agcgtcgtag ggatttgctg gtactcctgt
     5401 attatcaatg ctaattccag gcatcaattg tttaaaggaa tctcgtctag cttcagtccc
     5461 aaagtcataa cccaatgtac ctaaagcgta aactaaagtt tcaggattaa tatcgctagg
     5521 agcttggcta gcagtgacag aggaagcaac aggataagac ataggttgat tttgtgatgt
     5581 tatgtaggat tgcaaaaggt tttgaaaagc ggcaagatcg gcagaagtgg gtaaagaaac
     5641 atttgcatcg ggtgtagatg cggcttcagt ggaaattgcg tttggggttg gactgctaac
     5701 agaggctaag gctggtgttg atgcttccgt tgacgcagat tccatgacac cgtcaaactc
     5761 agaactcgtt acttctgaag gcgatatgct actgtctgct gctgaatgat tcatataatt
     5821 tatcgggtat aaagaatcgc ccttgttaat acctccgcat ccacaaccgg aggccgttaa
     5881 ggcttcttgt ccttgtgaca tttttcctcc tttgagtttt tccattgcac caagaatatt
     5941 tagtttgccg actaaacacc gggggcgatc gctttggttt tttagttcgc aagggatagc
     6001 actttgtaaa atagcgtctc taactttttg cgggtcgggt ttttctccac gttgctgctg
     6061 caaactcaat aacagcgctg caactcctga gacaatgggg gtagcaaagc ttgtgccagt
     6121 ccactcttta attccgcctc cggctttcgc ccccaaaata ttttcgcctg gtgcaagaat
     6181 gccttgagtt tgatatgtat cgccccagtt actaaaatca atgggctggc cgccatcgtc
     6241 catcgctccc actgccaaca cagccggcaa ggctgcggga acatgcaaac attcacagcc
     6301 gttattgcca gctgcggcca caagtaaaac attattctct ctacacaaag ccacagcttt
     6361 ttctaaccag ccatccgcct gaccatagtc agccagttgc ccaccactta gattaataat
     6421 gtgcgccccg gaattgactg cttgttcaat ggctcgtgcc agatctaatt gagataaatt
     6481 acgacctttg tcggaaaaaa tgggaataat caaacccctg catcgaggtg ctattccttc
     6541 aactggtgag ccgtgctgcc caaaaatcac gctggcaaca tgagttccat gtcgatgcat
     6601 cagcccttct gggttgggga tagattctac catgccggga atttctctca aatcggctcc
     6661 ctgaaagcaa atatgagagc gatcgactaa cccatctagc accgctacac aaatttccgg
     6721 tttgcccagt gtttgctccc acagatgtgt cagtccaggg attatcttga ctttagacat
     6781 gaggtagtcg caaaaaaagt tttagagtct agagaatttc aaacaactta gtttgagcat
     6841 tttcttatta cttaagtaat ttcaatagaa aactttatag tccatcgatt agaaatagcg
     6901 atcgcactca agctattttt tctagattga aaaattatat aggactattg tcaccaaaaa
     6961 tcaatagcca aattgctaaa aaatcaaaaa aaataacttt attaagtatc gttacaacaa
     7021 tcagctattt ctagcggaca gttaagacag ttaaggattt ttttttnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nncggacagt taagacactt aagaaaattc attcaaaata tttcttcaac aaagcttatt
     7201 gctaaccctt gttcatcgtg atataagaaa ttaaaagttt ccgttaaaga agttttgttt
     7261 aaaaaaactt tcttttagag aaacttttta ttcgatttac ttcattagga gaaatctctc
     7321 atgaaaccag aaaatcaaaa gcaaactcgt ttgcaaccca aactttctga acctacagtg
     7381 cgtgttatgc ctggtataca actcgactta tcagaagaag agttgacaaa tggtggcgta
     7441 ggagttggcg gtgtaaacgc ttctcaatac ggtggtggag acggtggtgt atctccctca
     7501 atcccatcaa ccagtggtgt ataactaact caaaagcaga tatatcaatt tgcttaacag
     7561 ttgaaaattt tttcacttca tttaagtact ttattagtac caataaactg atagctcggc
     7621 tacgtcacta actcgtttaa taacacttct aacgagtaag ttgtctgact tttaatatca
     7681 gtttgaagtg aataaaaata ttagagaaac aaactctttg caaaagctta ctatcttagt
     7741 aggattagct tgggtttgtc ttctctaatt aaattgactc tctagtaaat tcccttcaaa
     7801 gtaagtaaat ctctgcgcct ctttgcgttt aactctgcgc tcctctgcgt taaaaaaaca
     7861 ttcctcctac acctgactta attataaatt tctcaatcat aaacaatttc ttgtaacata
     7921 aaaatagcaa tatgaaaatt ctccaaataa aaccccattt tcgcgccgaa attatcgaac
     7981 ccaagcacgt ctatttatta agtgagtctt ctacccatgc ccttaccgga gaactctact
     8041 gccaactgat cccccttctt aacggcaact ataccgttga tgaaattatc aataaactgc
     8101 aagtagaccc cagtcacatt gactatgccc tagaacgctt gcaagccaga ggctacctaa
     8161 ctgaagccac ccctcaactt actccagaag cagttgcttt ttggggtttg ctgaaagtag
     8221 aaccccaagt tgcttaccaa tgtttgcagc aaacccaagt ttatgtttct tcagttgcca
     8281 atcttcccac ccaatcgtta atcactgctt tagaagaagt aggaatcaaa gctatcaact
     8341 gggatggaga attacaagaa tttccgcctc attctctatt agttgtttta accgatgact
     8401 atctccaacc cgaactaaat aaaatcaatc aaatagccct caaaggcaat caaccttggt
     8461 tgctaatgaa acctgttggt acaattcttt ggttagggcc aatcttccaa cctcaaataa
     8521 caggctgttg ggaatgcctc gcccaacgtc tcaggattaa ccgagaagtg gaagcttcgg
     8581 tattaaggca aaaaaatagt tcattgcaac tttctcctag tcaagagtcg gactcttctg
     8641 tgcagcaaag gaatggaaat aaagtcaaat cagaggtaat tgaatgtctt cctcctcctc
     8701 cggcggtgat tccatctact ttacaaactg ccttgcagct agcaactaca gaaattgcta
     8761 aatggattgt taaacaaggt gtagaagaca caacaccctt ccctacctta gaaggaaaag
     8821 ttatcacctt tgaccaaaga aacttagata tccaaaccca cattcttagt ttacgtcccc
     8881 aatgtcccag ttgcggcaac cccaatctat tgaccgaacg ggcttttcaa cctttagttc
     8941 tcagcagccg caaaaagcaa tttaccagcg atggcggaca tcgtgctttc tctcccgacc
     9001 aaactgttaa tcgctatcaa cacctaatta gtcctattac aggagtggtc acttccctag
     9061 ttaggtcttc cgaccctgat gatagcttgc tccatagcta taatgctatc catagctttg
     9121 cagttgcttc caatatagga aaattgcgcc gttatcttaa gcataaaagt gggggcaaag
     9181 gaaaaactga ccgtcaatca aaagctagtg gattttgtga agcgatcgaa cgttattctg
     9241 gtgtttacca aggaaacgaa ccccgcatta gtgccacttt agccgagtta ggagataaag
     9301 ccatccatcc agcaaagtgt tcgcttttta gttccgaaca atatgaatat cgtgaagaat
     9361 tcaaccgcag aggtggagta tttgattgga ttcctcaacc cttcgatgaa actaaagtca
     9421 ttgaatggac tcccgtttgg tctttaaccg agcaaaccca caaatacatt cccacggctt
     9481 attgttacta cggctatcct ctaccggaag accatgaatt ttgtcgtgcc aattctaatg
     9541 gcgatgccac tggtaatact ctcgaagaag caattctgca aggattcttt gaaatagtcg
     9601 aacgggatag tatatcaatt tggtggtata accgccttaa gcgtccccgt gtggatttag
     9661 ctagttttaa cgaaccatat ctgctggaag tgcaagattt atataaaagc aaaaatcgtg
     9721 acttatgggt actggatgtc acagcagatt tagggattcc cacttttgtg gctgtctcct
     9781 atcttaaagg taaggcacag caaacaattc ttctgggttt tggaacgcat tttgacccta
     9841 aaattgccat cttgcgggca gttaccgaag tgaatcaagt cgggttcact tgtgatgatg
     9901 cagaggtgaa taaagagttt ggggaaatgc gagaatggtt taaaaaagca accattgaaa
     9961 atcagcctta tttagttcca gactctacag ttccagcaaa agtatatcaa gattatcaac
    10021 tgcgttggag cgatgatatc tatcaagatg tcatgacttg tgtagaaatt gccaaaaacg
    10081 ctggattaga aactttagta ctagataaaa ctcgcccaga tatcggctta aatgtggtga
    10141 aagttattgt gccagaaatg ccacattact ggctgaggat gggggcaaaa cgcatctatg
    10201 atgttcctgt aaaaatgggc tggttatcta caccgttaac agaagaacaa atgaatccga
    10261 tatcagtgcc aatttaaatc aaggcaagta actttcatgc ccttagtcat acttgtgggg
    10321 tgggtatatt gcttgtgggg tgggcatctt gcccgccata taaactgggc aggcgagacg
    10381 cccagtgtac aaggaataat tgaatattct tattttgaaa tttctaaaca aatgtttgct
    10441 tgtagtgtac ccctacgggg aagcaagcta cgcgtagcgt cttgcacaga ggactaaagt
    10501 cttcactacg aacgatttaa tttttattta ctattttagt taataccatg aactcagctt
    10561 tcaccatttc tttagcaaaa aaaattgact taattccaga gaatgagcgc attattctta
    10621 aatctaatac ttataaatta actttatctc acccacaacc tggtttaaaa tctgcattca
    10681 accatctcaa gcattcccct gctactctct ctgacctcag acaattagtt gtagaaactg
    10741 atggaattga aggaaaagat aaatttgata aatatctgca aggactaact aacttgggct
    10801 ggatttgtca ttcagttttt cctcttgcta ctgctatccc gatgacggga aactatcaaa
    10861 ttaactatcc acaaattcaa aattggcaac aacaaacatt tactctatct cggtttgcct
    10921 atttgcatca agtaaacggg gaaatggttt tagaatctcc ccagtcccaa gccaaaatca
    10981 ttttattaga ttggcgagcc acagccttaa ttactaaatt atctcagccc caaactagca
    11041 caacattaat agcaggaatt ccagacatca cagaagaact agcgcaacaa ttcctcagtc
    11101 tacttttagc tactcaaatg ctatcttcag aaaccgaaaa ttctcatctc atgcaatggg
    11161 aatttcacga cctcctcttc cacagtcgca gtcgtaaagg tagacatagc aatcctgtag
    11221 ggggaacata tcgtttctta gataaaattg agcctctacc tgtggtcaag ccgcccatga
    11281 gtaacaaggt gattgaatta tataaacccg atttggaaga tttagcaaaa acagatattt
    11341 ctttcaccca agtattagaa tcaagacggt caattagaga gtacggcgaa gacccaatca
    11401 ccatagaaca attaggagaa ttattatatc gctctgctag aatccaagag gtaattgata
    11461 aagaaaatat gggacaatta agccgtcgtc cttatcccgg tggcggtgct attcatgagt
    11521 tagagattta tccactaatt aactactgtg aaggactaga agcagcacta tatcattatc
    11581 gtcctgatgc ccatgaatta gaattagttt ctgagtggag tcaagaagta gaagctttag
    11641 tcacagaagc ttgtttatct aatggtagcc agaaaatgcc gcaagtttta attataatta
    11701 ctgcgagatt caaccgcatg ttttggaaat accaatcaat ggcctacgct gcaattttaa
    11761 aacacgttgg agttttatac caaactttgt atttagtatc aactgcaatg aatttagcac
    11821 cctgtggaat aggtgcgggt aattcagata tctttgctaa agcaacggga attgactact
    11881 atgaagaatc ttctgtggga gagtttatac taggcacgaa ataatctttg tgacgtagtt
    11941 tgcgaaatta gaaataaatt attcaatctt gtgaggtggg tatattgcgt agactgggcg
    12001 ggcaagatgt ccaccccaca agagttaatt atattttttt atttacaagt ttcttataca
    12061 aaaaatctcc agttttgata tgaataaaca actagaatca ttacaatcaa ttcttgtatt
    12121 tgatggacaa gacaactcga taaatttagg caaaaaacct gaatttaaaa ttgagaagaa
    12181 aattacttta gaagcttggg tttattgtca aaatcaacga cgacgcactg gcattatttc
    12241 taatattttt gacacaagtg acaaagaaag tggatatggt ttgcttctcg atggcaagag
    12301 tggtgtattt tttgctttaa aaactacatc tcaaaaaatt cattatctta gtagtaaggc
    12361 tgatagttta aagctaaatc aatggcatca tctagctgga acctacgatg gtaaacagat
    12421 aaaggtgtat attgatggtg tagaaaaagc cgctaaagca attgccaatt ctgcaattaa
    12481 ttacgaacca gaaaatgatt tattcattgg gacgtacaaa gataataacg aaacttatcc
    12541 ttttcaaggt aaaattgctg aagttcgcat ttggaaagta gcccgaactc aacaacaaat
    12601 ccaaaataat ctttaccaac gtcttgcagg aaatgaaccg ggattaattg gttattgggc
    12661 tttaaatgaa ggtagtgaaa atgtagctca tgacggtact accaatgcca atcatggtaa
    12721 tattaacggt gctaactggg aacagtcaca agtggcattt actaattctc aatctcaaga
    12781 gttagtagtt atcaaaaccc taactacaga aaaagaaact accttagaag taaaaccagt
    12841 agaacagaaa ttaacccaaa attcagagaa agcaaaagag attgaaaaac atcttttagc
    12901 cactggactt gaagattatg gattttggtg gcaagaaatg ttgaaagcgc aagctcaaaa
    12961 aactgagctg gataaacctt ttcgtcgcgg tcggatttgg gcttaatata acagaattta
    13021 gaggctggaa attttgggag atatggggat tggtgttgat tgttgactgt tgactcttaa
    13081 taagtagata gtcatgatta aatctaaaat gatacaaata caagcgtaag ggcgaacagt
    13141 aagggcgaat agccgttcgc ccgtacaggt tattgtatgt gtatcaatat tttggtgaat
    13201 tagtataaca tgtgatgttt cacaaattat ttgagattgc tatatatcaa tacggttggt
    13261 gttaaaggct aattctacaa aattgtgggt ttaccagacg cgataaatcg ccgtctctat
    13321 aagtgttttg gtcttaagta gatggacata aataaatata
//
